LOCUS       NM_021173               1694 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens DNA polymerase delta 4, accessory subunit (POLD4),
            transcript variant 1, mRNA.
ACCESSION   NM_021173
VERSION     NM_021173.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Alam,M.T., Ali,M.S., Goel,H., Singh,J., Chatterjee,B., Bose,S.,
            Hadda,V. and Chopra,A.
  TITLE     Expression Profile, Molecular Association, and Clinical
            Significance of POLD4 in Glioblastoma
  JOURNAL   Cell Mol Neurobiol 43 (7), 3753-3765 (2023)
   PUBMED   37543966
  REMARK    GeneRIF: Expression Profile, Molecular Association, and Clinical
            Significance of POLD4 in Glioblastoma.
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Jiang,C., Fan,F., Xu,W. and Jiang,X.
  TITLE     POLD4 Promotes Glioma Cell Proliferation and Suppressive Immune
            Microenvironment: A Pan-Cancer Analysis Integrated with
            Experimental Validation
  JOURNAL   Int J Mol Sci 24 (18), 13919 (2023)
   PUBMED   37762224
  REMARK    GeneRIF: POLD4 Promotes Glioma Cell Proliferation and Suppressive
            Immune Microenvironment: A Pan-Cancer Analysis Integrated with
            Experimental Validation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1694)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 1694)
  AUTHORS   Zhang,Z., Yu,X., Zhou,B., Zhang,J. and Chang,J.
  TITLE     Circular RNA circ_0026359 Enhances Cisplatin Resistance in Gastric
            Cancer via Targeting miR-1200/POLD4 Pathway
  JOURNAL   Biomed Res Int 2020, 5103272 (2020)
   PUBMED   32855967
  REMARK    GeneRIF: Circular RNA circ_0026359 Enhances Cisplatin Resistance in
            Gastric Cancer via Targeting miR-1200/POLD4 Pathway.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1694)
  AUTHORS   Selak,N., Bachrati,C.Z., Shevelev,I., Dietschy,T., van Loon,B.,
            Jacob,A., Hubscher,U., Hoheisel,J.D., Hickson,I.D. and Stagljar,I.
  TITLE     The Bloom's syndrome helicase (BLM) interacts physically and
            functionally with p12, the smallest subunit of human DNA polymerase
            delta
  JOURNAL   Nucleic Acids Res 36 (16), 5166-5179 (2008)
   PUBMED   18682526
  REMARK    GeneRIF: The DNA polymerase delta enzyme, as well as the isolated
            p12 subunit, stimulates the DNA helicase activity of Bloom's
            syndrome helicase.
REFERENCE   6  (bases 1 to 1694)
  AUTHORS   Xie,B., Mazloum,N., Liu,L., Rahmeh,A., Li,H. and Lee,M.Y.
  TITLE     Reconstitution and characterization of the human DNA polymerase
            delta four-subunit holoenzyme
  JOURNAL   Biochemistry 41 (44), 13133-13142 (2002)
   PUBMED   12403614
REFERENCE   7  (bases 1 to 1694)
  AUTHORS   Bae,S.H., Bae,K.H., Kim,J.A. and Seo,Y.S.
  TITLE     RPA governs endonuclease switching during processing of Okazaki
            fragments in eukaryotes
  JOURNAL   Nature 412 (6845), 456-461 (2001)
   PUBMED   11473323
REFERENCE   8  (bases 1 to 1694)
  AUTHORS   Liu,L., Mo,J., Rodriguez-Belmonte,E.M. and Lee,M.Y.
  TITLE     Identification of a fourth subunit of mammalian DNA polymerase
            delta
  JOURNAL   J Biol Chem 275 (25), 18739-18744 (2000)
   PUBMED   10751307
REFERENCE   9  (bases 1 to 1694)
  AUTHORS   Matsumoto,Y., Kim,K., Hurwitz,J., Gary,R., Levin,D.S.,
            Tomkinson,A.E. and Park,M.S.
  TITLE     Reconstitution of proliferating cell nuclear antigen-dependent
            repair of apurinic/apyrimidinic sites with purified human proteins
  JOURNAL   J Biol Chem 274 (47), 33703-33708 (1999)
   PUBMED   10559261
REFERENCE   10 (bases 1 to 1694)
  AUTHORS   Wood,R.D. and Shivji,M.K.
  TITLE     Which DNA polymerases are used for DNA-repair in eukaryotes?
  JOURNAL   Carcinogenesis 18 (4), 605-610 (1997)
   PUBMED   9111189
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC070250.1, AF179890.1 and
            AP003419.3.
            
            On Nov 23, 2018 this sequence version replaced NM_021173.4.
            
            Summary: This gene encodes the smallest subunit of DNA polymerase
            delta. DNA polymerase delta possesses both polymerase and 3' to 5'
            exonuclease activity and plays a critical role in DNA replication
            and repair. The encoded protein enhances the activity of DNA
            polymerase delta and plays a role in fork repair and stabilization
            through interactions with the DNA helicase Bloom syndrome protein.
            Alternatively spliced transcript variants encoding multiple
            isoforms have been observed for this gene. [provided by RefSeq, Mar
            2012].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC070250.1, SRR3476690.800184.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000312419.8/ ENSP00000311368.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-73                BC070250.1         1-73
            74-585              AF179890.1         1-512
            586-1694            AP003419.3         31237-32345         c
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.2"
     gene            1..1694
                     /gene="POLD4"
                     /gene_synonym="p12; POLDS"
                     /note="DNA polymerase delta 4, accessory subunit"
                     /db_xref="GeneID:57804"
                     /db_xref="HGNC:HGNC:14106"
                     /db_xref="MIM:611525"
     exon            1..244
                     /gene="POLD4"
                     /gene_synonym="p12; POLDS"
                     /inference="alignment:Splign:2.1.0"
     CDS             148..471
                     /gene="POLD4"
                     /gene_synonym="p12; POLDS"
                     /note="isoform 1 is encoded by transcript variant 1; DNA
                     polymerase delta smallest subunit p12; DNA polymerase
                     delta subunit 4; polymerase (DNA-directed), delta 4,
                     accessory subunit; polymerase (DNA) delta 4, accessory
                     subunit"
                     /codon_start=1
                     /product="DNA polymerase delta subunit 4 isoform 1"
                     /protein_id="NP_066996.3"
                     /db_xref="CCDS:CCDS8158.1"
                     /db_xref="GeneID:57804"
                     /db_xref="HGNC:HGNC:14106"
                     /db_xref="MIM:611525"
                     /translation="MGRKRLITDSYPVVKRREGPAGHSKGELAPELGEEPQPRDEEEA
                     ELELLRQFDLAWQYGPCTGITRLQRWCRAKQMGLEPPPEVWQVLKTHPGDPRFQCSLW
                     HLYPL"
     exon            245..334
                     /gene="POLD4"
                     /gene_synonym="p12; POLDS"
                     /inference="alignment:Splign:2.1.0"
     exon            335..446
                     /gene="POLD4"
                     /gene_synonym="p12; POLDS"
                     /inference="alignment:Splign:2.1.0"
     exon            447..1694
                     /gene="POLD4"
                     /gene_synonym="p12; POLDS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      896..901
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLD4"
                     /gene_synonym="p12; POLDS"
                     /note="hexamer: AATAAA"
     polyA_site      918
                     /gene="POLD4"
                     /gene_synonym="p12; POLDS"
                     /note="major polyA site"
     regulatory      1674..1679
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLD4"
                     /gene_synonym="p12; POLDS"
                     /note="hexamer: AATAAA"
     polyA_site      1694
                     /gene="POLD4"
                     /gene_synonym="p12; POLDS"
ORIGIN      
        1 ctctctcggt ttgtctgggt catcttgtct gcccgccgct ggcctggccc cgtctgtctc
       61 tctcagcagc tgtctttctc gcgcccactg gccggtctct cctcttcccc gcagttgcct
      121 ccttctctgc ctgcctgggt ggccgccatg ggccggaagc ggctcatcac tgattcctac
      181 ccggttgtga agaggaggga ggggcccgct gggcacagca agggggagct ggcacccgag
      241 ctaggggagg agccccagcc ccgcgacgag gaggaagcgg agctggagct gctgaggcag
      301 tttgacctgg cctggcagta cgggccctgc accgggatca cacggctgca gcgctggtgt
      361 cgggccaagc agatgggctt ggagcctccc ccagaggtgt ggcaggtgct gaagacccac
      421 cccggagacc cccgcttcca gtgcagtctc tggcatctct atcccctatg aggcaccacg
      481 taagacctcc tgcccttagc tctcttgctc accacccaag aacctcagga cagaagcgag
      541 agcccattgc tcctgctcag ctcagcccgg ctgcggagga acccttggca ggcagaacct
      601 ggaggtgtca gaggctcaac tcctccatct aaccagcagg ctcccagagt ccccggaaga
      661 gcctgcgcag ctgaagcaga gtgcttctag atggagagtg gtcactgggg aaaaggacct
      721 ggccatcacc ttccaatacc tgctgcctgt ctccctgacc catgatctgg caagttaggc
      781 acagtcagac atggacagtt gatccatgag gaaaagatgc tctcccacct aaggccagga
      841 atctgagagc aggactggct gagctcccag ggcaaggggt tcactaatgc ttatcaataa
      901 agaatattga gcctggaatc ctgaccttca gtcctttact gtggccctgc tttgactggc
      961 agtccctagc aagttaggag atgagagagg caccactaca gctcttttcc ttcaagaaag
     1021 tcctaggccg aagagggagg agacagtcat cttgagacgg atcaagtcct cggaagagct
     1081 gagcgacctc tggtgccccc tggtggccca cagggaaact actcatgact caccttctgg
     1141 agcagccagg gaaaacctac ctgactagcc agccacctgc cttgctaagc ggtgcccacc
     1201 tcacaaacat gggtgctttg ccagagaagg acccctgggg tccatagctc atgaagtcca
     1261 aacatatcta tacattccca gcaagcacct ctggcttccc acatcggggg ccaccttgat
     1321 ccaaagcagg gctggcgagg gtctcaactt gcaggacctg ctcagacatg accatcatct
     1381 gaggggcttt ggtaagaaac tgatggctgg gcgcggtggc tcacacctgt aatcccagca
     1441 ctttgggagg ccaaggaggg cggatcacga ggtcaagaga tcgagaccat cctggctaac
     1501 atggtgaaac cccatctcta ctaaaaatac aaaaaattag ccaggtgtag tcccagctac
     1561 tcaggaggct gaggcaggag aatggcgtga acccgggagg cagagctggc agtgagctga
     1621 gaccgcatca ctgcactcca gcctgggtga cagagagaga ccccgtctca aaaaataaaa
     1681 aaatacaaaa taaa
//
