LOCUS       NM_006230               1609 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens DNA polymerase delta 2, accessory subunit (POLD2),
            transcript variant 2, mRNA.
ACCESSION   NM_006230
VERSION     NM_006230.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Krishnamoorthy,V., Foglizzo,M., Dilley,R.L., Wu,A., Datta,A.,
            Dutta,P., Campbell,L.J., Degtjarik,O., Musgrove,L.J.,
            Calabrese,A.N., Zeqiraj,E. and Greenberg,R.A.
  TITLE     The SPATA5-SPATA5L1 ATPase complex directs replisome proteostasis
            to ensure genome integrity
  JOURNAL   Cell 187 (9), 2250-2268 (2024)
   PUBMED   38554706
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Salehi,Z., Keramatipour,M., Talebi,S., Arab,S.S., Naser
            Moghadasi,A., Sahraian,M.A. and Izad,M.
  TITLE     Exome sequencing reveals novel rare variants in Iranian familial
            multiple sclerosis: The importance of POLD2 in the disease
            pathogenesis
  JOURNAL   Genomics 113 (4), 2645-2655 (2021)
   PUBMED   34116171
  REMARK    GeneRIF: Exome sequencing reveals novel rare variants in Iranian
            familial multiple sclerosis: The importance of POLD2 in the disease
            pathogenesis.
REFERENCE   3  (bases 1 to 1609)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 1609)
  AUTHORS   Layer,J.V., Debaize,L., Van Scoyk,A., House,N.C., Brown,A.J.,
            Liu,Y., Stevenson,K.E., Hemann,M., Roberts,S.A., Price,B.D.,
            Weinstock,D.M. and Day,T.A.
  TITLE     Polymerase delta promotes chromosomal rearrangements and imprecise
            double-strand break repair
  JOURNAL   Proc Natl Acad Sci U S A 117 (44), 27566-27577 (2020)
   PUBMED   33077594
  REMARK    GeneRIF: Polymerase delta promotes chromosomal rearrangements and
            imprecise double-strand break repair.
REFERENCE   5  (bases 1 to 1609)
  AUTHORS   Elgaaen,B.V., Haug,K.B., Wang,J., Olstad,O.K., Fortunati,D.,
            Onsrud,M., Staff,A.C., Sauer,T. and Gautvik,K.M.
  TITLE     POLD2 and KSP37 (FGFBP2) correlate strongly with histology, stage
            and outcome in ovarian carcinomas
  JOURNAL   PLoS One 5 (11), e13837 (2010)
   PUBMED   21079801
  REMARK    GeneRIF: Results suggest that POLD2 and KSP37 might be potential
            prognostic biomarkers.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1609)
  AUTHORS   Hisama,F.M., Chen,Y.H., Meyn,M.S., Oshima,J. and Weissman,S.M.
  TITLE     WRN or telomerase constructs reverse 4-nitroquinoline 1-oxide
            sensitivity in transformed Werner syndrome fibroblasts
  JOURNAL   Cancer Res 60 (9), 2372-2376 (2000)
   PUBMED   10811112
REFERENCE   7  (bases 1 to 1609)
  AUTHORS   Matsumoto,Y., Kim,K., Hurwitz,J., Gary,R., Levin,D.S.,
            Tomkinson,A.E. and Park,M.S.
  TITLE     Reconstitution of proliferating cell nuclear antigen-dependent
            repair of apurinic/apyrimidinic sites with purified human proteins
  JOURNAL   J Biol Chem 274 (47), 33703-33708 (1999)
   PUBMED   10559261
REFERENCE   8  (bases 1 to 1609)
  AUTHORS   Hindges,R. and Hubscher,U.
  TITLE     Cloning, chromosomal localization, and interspecies interaction of
            mouse DNA polymerase delta small subunit (PolD2)
  JOURNAL   Genomics 44 (1), 45-51 (1997)
   PUBMED   9286699
REFERENCE   9  (bases 1 to 1609)
  AUTHORS   Wood,R.D. and Shivji,M.K.
  TITLE     Which DNA polymerases are used for DNA-repair in eukaryotes?
  JOURNAL   Carcinogenesis 18 (4), 605-610 (1997)
   PUBMED   9111189
  REMARK    Review article
REFERENCE   10 (bases 1 to 1609)
  AUTHORS   Zhang,J., Tan,C.K., McMullen,B., Downey,K.M. and So,A.G.
  TITLE     Cloning of the cDNAs for the small subunits of bovine and human DNA
            polymerase delta and chromosomal location of the human gene (POLD2)
  JOURNAL   Genomics 29 (1), 179-186 (1995)
   PUBMED   8530069
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC006454.4.
            
            On Jul 14, 2020 this sequence version replaced NM_006230.3.
            
            Summary: This gene encodes the 50-kDa catalytic subunit of DNA
            polymerase delta. DNA polymerase delta possesses both polymerase
            and 3' to 5' exonuclease activity and plays a critical role in DNA
            replication and repair. The encoded protein is required for the
            stimulation of DNA polymerase delta activity by the processivity
            cofactor proliferating cell nuclear antigen (PCNA). Expression of
            this gene may be a marker for ovarian carcinomas. Alternatively
            spliced transcript variants encoding multiple isoforms have been
            observed for this gene, and a pseudogene of this gene is located on
            the long arm of chromosome 5. [provided by RefSeq, Mar 2012].
            
            Transcript Variant: This variant (2) uses an alternate splice site
            in the 5' region and initiates translation at an upstream start
            codon, compared to variant 1. The encoded isoform (2) has a longer
            N-terminus, compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC000459.2, U21090.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000610533.6/ ENSP00000480186.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-38                AC006454.4         129052-129089
            39-314              AC006454.4         130491-130766
            315-436             AC006454.4         134536-134657
            437-560             AC006454.4         134858-134981
            561-675             AC006454.4         135353-135467
            676-874             AC006454.4         135585-135783
            875-955             AC006454.4         136090-136170
            956-1113            AC006454.4         136328-136485
            1114-1241           AC006454.4         136707-136834
            1242-1343           AC006454.4         137204-137305
            1344-1609           AC006454.4         137655-137920
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p13"
     gene            1..1609
                     /gene="POLD2"
                     /note="DNA polymerase delta 2, accessory subunit"
                     /db_xref="GeneID:5425"
                     /db_xref="HGNC:HGNC:9176"
                     /db_xref="MIM:600815"
     exon            1..38
                     /gene="POLD2"
                     /inference="alignment:Splign:2.1.0"
     exon            39..314
                     /gene="POLD2"
                     /inference="alignment:Splign:2.1.0"
     CDS             95..1504
                     /gene="POLD2"
                     /EC_number="2.7.7.7"
                     /note="DNA polymerase delta subunit p50; Pol delta B
                     subunit (p50); polymerase (DNA directed), delta 2,
                     regulatory subunit 50kDa; polymerase (DNA directed), delta
                     2, accessory subunit; polymerase (DNA) delta 2, accessory
                     subunit; DAN polymerase delta 2, accessory subunit"
                     /codon_start=1
                     /product="DNA polymerase delta subunit 2"
                     /protein_id="NP_006221.3"
                     /db_xref="CCDS:CCDS5477.1"
                     /db_xref="GeneID:5425"
                     /db_xref="HGNC:HGNC:9176"
                     /db_xref="MIM:600815"
                     /translation="MFSEQAAQRAHTLLSPPSANNATFARVPVATYTNSSQPFRLGER
                     SFSRQYAHIYATRLIQMRPFLENRAQQHWGSGVGVKKLCELQPEEKCCVVGTLFKAMP
                     LQPSILREVSEEHNLLPQPPRSKYIHPDDELVLEDELQRIKLKGTIDVSKLVTGTVLA
                     VFGSVRDDGKFLVEDYCFADLAPQKPAPPLDTDRFVLLVSGLGLGGGGGESLLGTQLL
                     VDVVTGQLGDEGEQCSAAHVSRVILAGNLLSHSTQSRDSINKAKYLTKKTQAASVEAV
                     KMLDEILLQLSASVPVDVMPGEFDPTNYTLPQQPLHPCMFPLATAYSTLQLVTNPYQA
                     TIDGVRFLGTSGQNVSDIFRYSSMEDHLEILEWTLRVRHISPTAPDTLGCYPFYKTDP
                     FIFPECPHVYFCGNTPSFGSKIIRGPEDQTVLLVTVPDFSATQTACLVNLRSLACQPI
                     SFSGFGAEDDDLGGLGLGP"
     exon            315..436
                     /gene="POLD2"
                     /inference="alignment:Splign:2.1.0"
     exon            437..560
                     /gene="POLD2"
                     /inference="alignment:Splign:2.1.0"
     exon            561..675
                     /gene="POLD2"
                     /inference="alignment:Splign:2.1.0"
     exon            676..874
                     /gene="POLD2"
                     /inference="alignment:Splign:2.1.0"
     exon            875..955
                     /gene="POLD2"
                     /inference="alignment:Splign:2.1.0"
     exon            956..1113
                     /gene="POLD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1114..1241
                     /gene="POLD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1242..1343
                     /gene="POLD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1344..1609
                     /gene="POLD2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1575..1580
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLD2"
                     /note="hexamer: AATAAA"
     polyA_site      1609
                     /gene="POLD2"
                     /note="major polyA site"
ORIGIN      
        1 aggcgcgcgg ggattggcgg gatgcggcgc gccgcgcgtg aacctccttg gcctgggcga
       61 agctgtgtgg accaagcaag tcaggagtgt ggccatgttt tctgagcagg ctgcccagag
      121 ggcccacact ctactgtccc caccatcagc caacaatgcc acctttgccc gggtgccagt
      181 ggcaacctac accaactcct cacaaccctt ccggctagga gagcgcagct ttagccggca
      241 gtatgcccac atttatgcca cccgcctcat ccaaatgaga cccttcctgg agaaccgggc
      301 ccagcagcac tggggcagtg gagtgggagt gaagaagctg tgtgaactgc agcctgagga
      361 gaagtgctgt gtggtgggca ctctgttcaa ggccatgccg ctgcagccct ccatcctgcg
      421 ggaggtcagc gaggagcaca acctgctccc ccagcctcct cggagtaaat acatacaccc
      481 agatgacgag ctggtcttgg aagatgaact gcagcgtatc aaactaaaag gcaccattga
      541 cgtgtcaaag ctggttacgg ggactgtcct ggctgtgttt ggctccgtga gagacgacgg
      601 gaagtttctg gtggaggact attgctttgc tgaccttgct ccccagaagc ccgcaccccc
      661 acttgacaca gataggtttg tgctactggt gtccggcctg ggcctgggtg gcggtggagg
      721 cgagagcctg ctgggcaccc agctgctggt ggatgtggtg acggggcagc ttggggacga
      781 aggggagcag tgcagcgccg cccacgtctc ccgggttatc ctcgctggca acctcctcag
      841 ccacagcacc cagagcaggg attctatcaa taaggccaaa tacctcacca agaaaaccca
      901 ggcagccagc gtggaggctg ttaagatgct ggatgagatc ctcctgcagc tgagcgcctc
      961 agtgcccgtg gacgtgatgc caggcgagtt tgatcccacc aattacacgc tcccccagca
     1021 gcccctccac ccctgcatgt tcccgctggc cactgcctac tccacgctcc agctggtcac
     1081 caacccctac caggccacca ttgatggagt cagatttttg gggacatcag gacagaacgt
     1141 gagtgacatt ttccgataca gcagcatgga ggatcacttg gagatcctgg agtggaccct
     1201 gcgggtccgt cacatcagcc ccacagcccc tgacactcta ggttgttacc ccttctacaa
     1261 aactgacccg ttcatcttcc cagagtgccc gcatgtctac ttttgtggca acacccccag
     1321 ctttggctcc aaaatcatcc gaggtcctga ggaccagaca gtgctgttgg tgactgtccc
     1381 tgacttcagt gccacgcaga ccgcctgcct tgtgaacctg cgcagcctgg cctgccagcc
     1441 catcagcttc tcgggcttcg gggcagagga cgatgacctg ggaggcctgg ggctgggccc
     1501 ctgactcaaa aaagtggttt tgaccagaga ggcccagatg gaggctgttc attccctgca
     1561 gtgtcggcat tgtaaataaa gcctgagcac ttgctgatgc gagccttga
//
