LOCUS       NM_002689               3544 bp    mRNA    linear   PRI 12-MAY-2024
DEFINITION  Homo sapiens DNA polymerase alpha 2, accessory subunit (POLA2),
            mRNA.
ACCESSION   NM_002689
VERSION     NM_002689.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Xie,F., Cheng,Y., Gao,X., Song,S., Zhao,X., Meng,X., Shang,Z. and
            Yang,M.
  TITLE     CircRNA circ_POLA2 overexpression suppresses cell apoptosis by
            downregulating PTEN in glioblastoma
  JOURNAL   Anticancer Drugs 34 (5), 652-658 (2023)
   PUBMED   36730613
  REMARK    GeneRIF: CircRNA circ_POLA2 overexpression suppresses cell
            apoptosis by downregulating PTEN in glioblastoma.
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Zhou,Y., Liu,Y., Gupta,S., Paramo,M.I., Hou,Y., Mao,C., Luo,Y.,
            Judd,J., Wierbowski,S., Bertolotti,M., Nerkar,M., Jehi,L.,
            Drayman,N., Nicolaescu,V., Gula,H., Tay,S., Randall,G., Wang,P.,
            Lis,J.T., Feschotte,C., Erzurum,S.C., Cheng,F. and Yu,H.
  TITLE     A comprehensive SARS-CoV-2-human protein-protein interactome
            reveals COVID-19 pathobiology and potential host therapeutic
            targets
  JOURNAL   Nat Biotechnol 41 (1), 128-139 (2023)
   PUBMED   36217030
REFERENCE   3  (bases 1 to 3544)
  AUTHORS   Chen,Z., Wang,C., Feng,X., Nie,L., Tang,M., Zhang,H., Xiong,Y.,
            Swisher,S.K., Srivastava,M. and Chen,J.
  TITLE     Interactomes of SARS-CoV-2 and human coronaviruses reveal host
            factors potentially affecting pathogenesis
  JOURNAL   EMBO J 40 (17), e107776 (2021)
   PUBMED   34232536
REFERENCE   4  (bases 1 to 3544)
  AUTHORS   Gordon,D.E., Hiatt,J., Bouhaddou,M., Rezelj,V.V., Ulferts,S.,
            Braberg,H., Jureka,A.S., Obernier,K., Guo,J.Z., Batra,J.,
            Kaake,R.M., Weckstein,A.R., Owens,T.W., Gupta,M., Pourmal,S.,
            Titus,E.W., Cakir,M., Soucheray,M., McGregor,M., Cakir,Z., Jang,G.,
            O'Meara,M.J., Tummino,T.A., Zhang,Z., Foussard,H., Rojc,A.,
            Zhou,Y., Kuchenov,D., Huttenhain,R., Xu,J., Eckhardt,M.,
            Swaney,D.L., Fabius,J.M., Ummadi,M., Tutuncuoglu,B., Rathore,U.,
            Modak,M., Haas,P., Haas,K.M., Naing,Z.Z.C., Pulido,E.H., Shi,Y.,
            Barrio-Hernandez,I., Memon,D., Petsalaki,E., Dunham,A.,
            Marrero,M.C., Burke,D., Koh,C., Vallet,T., Silvas,J.A.,
            Azumaya,C.M., Billesbolle,C., Brilot,A.F., Campbell,M.G.,
            Diallo,A., Dickinson,M.S., Diwanji,D., Herrera,N., Hoppe,N.,
            Kratochvil,H.T., Liu,Y., Merz,G.E., Moritz,M., Nguyen,H.C.,
            Nowotny,C., Puchades,C., Rizo,A.N., Schulze-Gahmen,U., Smith,A.M.,
            Sun,M., Young,I.D., Zhao,J., Asarnow,D., Biel,J., Bowen,A.,
            Braxton,J.R., Chen,J., Chio,C.M., Chio,U.S., Deshpande,I., Doan,L.,
            Faust,B., Flores,S., Jin,M., Kim,K., Lam,V.L., Li,F., Li,J.,
            Li,Y.L., Li,Y., Liu,X., Lo,M., Lopez,K.E., Melo,A.A., Moss,F.R.
            3rd, Nguyen,P., Paulino,J., Pawar,K.I., Peters,J.K., Pospiech,T.H.
            Jr., Safari,M., Sangwan,S., Schaefer,K., Thomas,P.V., Thwin,A.C.,
            Trenker,R., Tse,E., Tsui,T.K.M., Wang,F., Whitis,N., Yu,Z.,
            Zhang,K., Zhang,Y., Zhou,F., Saltzberg,D., Hodder,A.J.,
            Shun-Shion,A.S., Williams,D.M., White,K.M., Rosales,R., Kehrer,T.,
            Miorin,L., Moreno,E., Patel,A.H., Rihn,S., Khalid,M.M.,
            Vallejo-Gracia,A., Fozouni,P., Simoneau,C.R., Roth,T.L., Wu,D.,
            Karim,M.A., Ghoussaini,M., Dunham,I., Berardi,F., Weigang,S.,
            Chazal,M., Park,J., Logue,J., McGrath,M., Weston,S., Haupt,R.,
            Hastie,C.J., Elliott,M., Brown,F., Burness,K.A., Reid,E.,
            Dorward,M., Johnson,C., Wilkinson,S.G., Geyer,A., Giesel,D.M.,
            Baillie,C., Raggett,S., Leech,H., Toth,R., Goodman,N., Keough,K.C.,
            Lind,A.L., Klesh,R.J., Hemphill,K.R., Carlson-Stevermer,J., Oki,J.,
            Holden,K., Maures,T., Pollard,K.S., Sali,A., Agard,D.A., Cheng,Y.,
            Fraser,J.S., Frost,A., Jura,N., Kortemme,T., Manglik,A.,
            Southworth,D.R., Stroud,R.M., Alessi,D.R., Davies,P., Frieman,M.B.,
            Ideker,T., Abate,C., Jouvenet,N., Kochs,G., Shoichet,B., Ott,M.,
            Palmarini,M., Shokat,K.M., Garcia-Sastre,A., Rassen,J.A.,
            Grosse,R., Rosenberg,O.S., Verba,K.A., Basler,C.F., Vignuzzi,M.,
            Peden,A.A., Beltrao,P. and Krogan,N.J.
  CONSRTM   QCRG Structural Biology Consortium; Zoonomia Consortium
  TITLE     Comparative host-coronavirus protein interaction networks reveal
            pan-viral disease mechanisms
  JOURNAL   Science 370 (6521) (2020)
   PUBMED   33060197
REFERENCE   5  (bases 1 to 3544)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (bases 1 to 3544)
  AUTHORS   Podust,V.N., Tiwari,N., Stephan,S. and Fanning,E.
  TITLE     Replication factor C disengages from proliferating cell nuclear
            antigen (PCNA) upon sliding clamp formation, and PCNA itself
            tethers DNA polymerase delta to DNA
  JOURNAL   J Biol Chem 273 (48), 31992-31999 (1998)
   PUBMED   9822671
REFERENCE   7  (bases 1 to 3544)
  AUTHORS   Stadlbauer,F., Brueckner,A., Rehfuess,C., Eckerskorn,C.,
            Lottspeich,F., Forster,V., Tseng,B.Y. and Nasheuer,H.P.
  TITLE     DNA replication in vitro by recombinant
            DNA-polymerase-alpha-primase
  JOURNAL   Eur J Biochem 222 (3), 781-793 (1994)
   PUBMED   8026492
REFERENCE   8  (bases 1 to 3544)
  AUTHORS   Collins,K.L., Russo,A.A., Tseng,B.Y. and Kelly,T.J.
  TITLE     The role of the 70 kDa subunit of human DNA polymerase alpha in DNA
            replication
  JOURNAL   EMBO J 12 (12), 4555-4566 (1993)
   PUBMED   8223465
REFERENCE   9  (bases 1 to 3544)
  AUTHORS   Nasheuer,H.P., Moore,A., Wahl,A.F. and Wang,T.S.
  TITLE     Cell cycle-dependent phosphorylation of human DNA polymerase alpha
  JOURNAL   J Biol Chem 266 (12), 7893-7903 (1991)
   PUBMED   1902230
REFERENCE   10 (bases 1 to 3544)
  AUTHORS   Wang,T.S., Hu,S.Z. and Korn,D.
  TITLE     DNA primase from KB cells. Characterization of a primase activity
            tightly associated with immunoaffinity-purified DNA
            polymerase-alpha
  JOURNAL   J Biol Chem 259 (3), 1854-1865 (1984)
   PUBMED   6693436
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC001347.2 and AP000944.4.
            
            On Nov 22, 2018 this sequence version replaced NM_002689.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.244848.1,
                                           SRR1803617.249355.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265465.8/ ENSP00000265465.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2476              BC001347.2         12-2487
            2477-3544           AP000944.4         38466-39533
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.1"
     gene            1..3544
                     /gene="POLA2"
                     /note="DNA polymerase alpha 2, accessory subunit"
                     /db_xref="GeneID:23649"
                     /db_xref="HGNC:HGNC:30073"
                     /db_xref="MIM:620063"
     exon            1..410
                     /gene="POLA2"
                     /inference="alignment:Splign:2.1.0"
     CDS             332..2128
                     /gene="POLA2"
                     /EC_number="2.7.7.7"
                     /note="polymerase (DNA-directed), alpha (70kD); DNA
                     polymerase alpha 70 kDa subunit; polymerase (DNA
                     directed), alpha 2 (70kD subunit); polymerase (DNA
                     directed), alpha 2, accessory subunit; polymerase (DNA)
                     alpha 2, accessory subunit"
                     /codon_start=1
                     /product="DNA polymerase alpha subunit B"
                     /protein_id="NP_002680.2"
                     /db_xref="CCDS:CCDS8098.1"
                     /db_xref="GeneID:23649"
                     /db_xref="HGNC:HGNC:30073"
                     /db_xref="MIM:620063"
                     /translation="MSASAQQLAEELQIFGLDCEEALIEKLVELCVQYGQNEEGMVGE
                     LIAFCTSTHKVGLTSEILNSFEHEFLSKRLSKARHSTCKDSGHAGARDIVSIQELIEV
                     EEEEEILLNSYTTPSKGSQKRAISTPETPLTKRSVSTRSPHQLLSPSSFSPSATPSQK
                     YNSRSNRGEVVTSFGLAQGVSWSGRGGAGNISLKVLGCPEALTGSYKSMFQKLPDIRE
                     VLTCKIEELGSELKEHYKIEAFTPLLAPAQEPVTLLGQIGCDSNGKLNNKSVILEGDR
                     EHSSGAQIPVDLSELKEYSLFPGQVVIMEGINTTGRKLVATKLYEGVPLPFYQPTEED
                     ADFEQSMVLVACGPYTTSDSITYDPLLDLIAVINHDRPDVCILFGPFLDAKHEQVENC
                     LLTSPFEDIFKQCLRTIIEGTRSSGSHLVFVPSLRDVHHEPVYPQPPFSYSDLSREDK
                     KQVQFVSEPCSLSINGVIFGLTSTDLLFHLGAEEISSSSGTSDRFSRILKHILTQRSY
                     YPLYPPQEDMAIDYESFYVYAQLPVTPDVLIIPSELRYFVKDVLGCVCVNPGRLTKGQ
                     VGGTFARLYLRRPAADGAERQSPCIAVQVVRI"
     exon            411..535
                     /gene="POLA2"
                     /inference="alignment:Splign:2.1.0"
     exon            536..627
                     /gene="POLA2"
                     /inference="alignment:Splign:2.1.0"
     exon            628..685
                     /gene="POLA2"
                     /inference="alignment:Splign:2.1.0"
     exon            686..792
                     /gene="POLA2"
                     /inference="alignment:Splign:2.1.0"
     exon            793..986
                     /gene="POLA2"
                     /inference="alignment:Splign:2.1.0"
     exon            987..1075
                     /gene="POLA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1076..1231
                     /gene="POLA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1232..1294
                     /gene="POLA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1295..1337
                     /gene="POLA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1338..1462
                     /gene="POLA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1463..1501
                     /gene="POLA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1502..1575
                     /gene="POLA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1576..1684
                     /gene="POLA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1685..1791
                     /gene="POLA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1792..1851
                     /gene="POLA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1852..1978
                     /gene="POLA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1979..3544
                     /gene="POLA2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2459..2464
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLA2"
                     /note="hexamer: ATTAAA"
     polyA_site      2476
                     /gene="POLA2"
                     /note="major polyA site"
     regulatory      3519..3524
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLA2"
                     /note="hexamer: AGTAAA"
     polyA_site      3544
                     /gene="POLA2"
ORIGIN      
        1 agttctgcca ccgtcactga gaagctcagc ggtagctttt gggaagcagg acgttctcac
       61 caggagagcg tcctctcgag atttctgctc cctccattca gggcgtttgg gagccacccc
      121 ttcatttttt aaaaaaagta tttctctgtg accgacggcc ggggccttct gacggtctga
      181 ggtcttgctt gggccagtca cctcctgtca cggtccgcgg aggggggaag gataagaggg
      241 cgaggagctc atcgctcgcc acccccgtgg gcttcttggg cgcaggtcgg agctgggtgg
      301 gccggctccc cggcccctgg cttgggcgac catgtccgca tccgcccagc agctggcgga
      361 ggagctgcag atcttcggcc tagactgcga ggaggctcta attgagaaat tggtagagct
      421 ttgtgttcag tatggacaga atgaggaggg aatggtaggc gagcttatag ccttctgcac
      481 cagcacacat aaagttggcc ttacctcaga gatcctgaac tcttttgagc atgagtttct
      541 gagcaaaaga ttatcgaaag ccaggcatag tacctgcaag gacagtggcc atgcaggagc
      601 tagagacatt gtttccattc aagagctaat tgaagtggaa gaagaagagg aaatcctctt
      661 gaactcttac accacacctt caaagggttc tcagaagcga gctatctcta ccccagaaac
      721 ccccctaaca aaaaggagtg tgtcaactcg tagcccccat cagctactct caccgtcaag
      781 tttctctcca agtgctactc cctcccagaa atacaactca cgaagtaacc gaggagaagt
      841 ggttacctcc ttcggcttag cacagggagt atcttggtct gggagaggag gagctggaaa
      901 catcagcctg aaggtcttgg gatgtccaga ggcactaact gggagctaca aatccatgtt
      961 tcagaagctc ccagacattc gagaagttct gacctgtaag atagaagaac ttggcagcga
     1021 actcaaggaa cattacaaga ttgaagcttt cactcctttg ctagccccag cacaggagcc
     1081 tgtcactctg ctgggccaga ttggctgtga tagcaacggg aagctgaaca acaagtcagt
     1141 gattctcgag ggagaccggg aacattcctc gggtgctcaa attccagtgg atttatctga
     1201 gcttaaggaa tattctctgt ttcctggaca ggttgtaatt atggaaggaa tcaacaccac
     1261 tggtaggaaa cttgttgcca ccaaactcta cgagggtgtg ccacttccat tttatcagcc
     1321 cactgaagag gatgcagact ttgagcaaag catggtcctg gttgcctgtg gaccatacac
     1381 cacatctgac agcatcacgt atgaccccct gcttgacctg attgctgtca tcaaccatga
     1441 ccggccagat gtctgcatcc tgtttggccc tttcctggat gctaagcatg aacaggtgga
     1501 gaattgtcta ctgacaagtc catttgaaga cattttcaag cagtgtctac gaacaattat
     1561 tgaaggcaca agaagctccg gctcccacct tgtctttgtc ccgtcattga gagatgtgca
     1621 ccatgagcct gtgtaccccc agccgccttt cagctactcc gatctgtctc gagaggacaa
     1681 aaagcaagta cagtttgtgt ccgagccctg cagcctctcc ataaacggag tgatcttcgg
     1741 cttgacatcc acagatctgc ttttccacct gggggccgag gagatcagta gttcttccgg
     1801 aacttcagac agattcagcc gaatactcaa gcacatcttg acccagagga gctactaccc
     1861 actctacccg ccccaagaag acatggccat tgactatgag tcgttctatg tttacgcaca
     1921 gctgcctgtc accccagatg tcctcatcat cccgtcagag ctgaggtact tcgtgaagga
     1981 tgtcctcggc tgtgtctgtg tgaaccctgg gcgccttacc aaagggcagg tgggaggcac
     2041 cttcgcccga ctctacctta ggaggccggc agcggacggg gcagagaggc agagcccatg
     2101 cattgctgtg caggtcgtca ggatctgagg cttctgtcct ctgctgttct ctgctgtgtg
     2161 ggcccttaaa gtcttagcca agagccaaga catagccctg tgacaaggtg aacagttggg
     2221 tgggaaagga gagaggagcc agccagggag gggcagctgc agtgaccagg cccagcaggg
     2281 aggacttgtg cagccgggcc tgcctctgag tggtgcctct cctggaagga agctcttgct
     2341 tctcagtcca tgctccgtgt ccagaagtaa gccagctgtg gatcccgccc actcagaaaa
     2401 ggcgagaagg ctttgtgatt ttctacatga atcaaacaca gaaacaactt ttggagaaat
     2461 taaattctga gtgtgacttc tgagtttgac ctttcttcta ttttctttta tttatttatt
     2521 ttgtttttag acggagtctc gatctgtcgc ccaggctgga gtgcagtggt gagatctcgg
     2581 ctcactgcaa gctctgccgc ccgggttcat gccattctcc tgcctcagcc tcctgagtag
     2641 ctgggactac aggcacccac caccacgcct ggctaatttt ttgtattttt agtggagatg
     2701 gggtttcacc atgttagcca ggatggtctc gatctcctga ccttgtgatc tgtccgcctc
     2761 agcctcccaa agtgctggga ttacaggcgt gagccaccgc gcccggcccc tttcttctat
     2821 tttctttatc ctggtagtag acaaagaatg ttgcctattt gacctatgtc cagagaaggc
     2881 agagtgtcag tcacactggg tttctatcca gccccagtcc tgtccagtgg gtaactctca
     2941 gccccactcc tcatccccca aaggccccag tgacctctat gctgcagagg tgcactgggt
     3001 gggccctaga gctgggctgc agagcaagca actggctgca tgcctgggcc tccctgcgca
     3061 gttcctcaca tgcatgctgt ggtcccagcc tgacctctcc atctggaggg ggccgcctcg
     3121 ctgggctgtt ccaaagctgg agtcctggtg gcagctgctt tgccaagtcg cgcagtagcc
     3181 agggcttgcc tgcagcccag aacactcaga cagggtcagg ccttccaggg ttcacagcac
     3241 gtcccatgcc cctatgtcct ggcatctggg gaaaagctgt cactggccag acgtggagtg
     3301 gcttcctgcc ctaagctggc aggctgtcct gcctcacatg ccagagccaa gaccggcaaa
     3361 ccccagctgt cagggcaggg aagtgggcaa gggggcagca agctgctggt gtctatgcct
     3421 tgactccgag ataaatagat gacctaacag tggccaactt ttgcctctca cctttctgac
     3481 ggtgaaatgg aatcgagccc cagacctgac cagggtacag taaaaggtct tcctttaaac
     3541 acaa
//
