LOCUS       NM_001330360            5487 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens DNA polymerase alpha 1, catalytic subunit (POLA1),
            transcript variant 1, mRNA.
ACCESSION   NM_001330360 XM_005274552
VERSION     NM_001330360.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Schimmel,J., Munoz-Subirana,N., Kool,H., van Schendel,R. and
            Tijsterman,M.
  TITLE     Small tandem DNA duplications result from CST-guided Pol
            alpha-primase action at DNA break termini
  JOURNAL   Nat Commun 12 (1), 4843 (2021)
   PUBMED   34376693
  REMARK    GeneRIF: Small tandem DNA duplications result from CST-guided Pol
            alpha-primase action at DNA break termini.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   Kelich,J.M., Papaioannou,H. and Skordalakes,E.
  TITLE     Pol alpha-primase dependent nuclear localization of the mammalian
            CST complex
  JOURNAL   Commun Biol 4 (1), 349 (2021)
   PUBMED   33731801
  REMARK    GeneRIF: Pol alpha-primase dependent nuclear localization of the
            mammalian CST complex.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5487)
  AUTHORS   Van Esch,H., Colnaghi,R., Freson,K., Starokadomskyy,P., Zankl,A.,
            Backx,L., Abramowicz,I., Outwin,E., Rohena,L., Faulkner,C.,
            Leong,G.M., Newbury-Ecob,R.A., Challis,R.C., Ounap,K., Jaeken,J.,
            Seuntjens,E., Devriendt,K., Burstein,E., Low,K.J. and O'Driscoll,M.
  TITLE     Defective DNA Polymerase alpha-Primase Leads to X-Linked
            Intellectual Disability Associated with Severe Growth Retardation,
            Microcephaly, and Hypogonadism
  JOURNAL   Am J Hum Genet 104 (5), 957-967 (2019)
   PUBMED   31006512
  REMARK    GeneRIF: Defective DNA Polymerase alpha-Primase Leads to X-Linked
            Intellectual Disability Associated with Severe Growth Retardation,
            Microcephaly, and Hypogonadism.
REFERENCE   4  (bases 1 to 5487)
  AUTHORS   Baranovskiy,A.G., Duong,V.N., Babayeva,N.D., Zhang,Y., Pavlov,Y.I.,
            Anderson,K.S. and Tahirov,T.H.
  TITLE     Activity and fidelity of human DNA polymerase alpha depend on
            primer structure
  JOURNAL   J Biol Chem 293 (18), 6824-6843 (2018)
   PUBMED   29555682
  REMARK    GeneRIF: detailed structural comparison of Polalpha with other
            replicative DNA polymerases disclosed common features and some
            differences, which may reflect the specialization of each
            polymerase in genome replication
REFERENCE   5  (bases 1 to 5487)
  AUTHORS   Podolska,A., Kobelt,A., Fuchs,S., Hackmann,K., Rump,A., Schrock,E.,
            Kutsche,K. and Di Donato,N.
  TITLE     Functional monosomy of 6q27-qter and functional disomy of
            Xpter-p22.11 due to X;6 translocation with an atypical
            X-inactivation pattern
  JOURNAL   Am J Med Genet A 173 (5), 1334-1341 (2017)
   PUBMED   28371302
  REMARK    GeneRIF: We propose that completely skewed XCI favoring the normal
            X chromosome resulted from death of cells with an active derivative
            X that was caused by a non-functional POLA1 gene. In summary, we
            conclude that functional monosomy of 6q27-qter and functional
            disomy of Xpter-p22.11 are responsible for the clinical phenotype
            of the patient
REFERENCE   6  (bases 1 to 5487)
  AUTHORS   Coverley,D., Kenny,M.K., Lane,D.P. and Wood,R.D.
  TITLE     A role for the human single-stranded DNA binding protein HSSB/RPA
            in an early stage of nucleotide excision repair
  JOURNAL   Nucleic Acids Res 20 (15), 3873-3880 (1992)
   PUBMED   1508673
REFERENCE   7  (bases 1 to 5487)
  AUTHORS   Dornreiter,I., Erdile,L.F., Gilbert,I.U., von Winkler,D.,
            Kelly,T.J. and Fanning,E.
  TITLE     Interaction of DNA polymerase alpha-primase with cellular
            replication protein A and SV40 T antigen
  JOURNAL   EMBO J 11 (2), 769-776 (1992)
   PUBMED   1311258
REFERENCE   8  (bases 1 to 5487)
  AUTHORS   Popanda,O. and Thielmann,H.W.
  TITLE     The function of DNA polymerases in DNA repair synthesis of
            ultraviolet-irradiated human fibroblasts
  JOURNAL   Biochim Biophys Acta 1129 (2), 155-160 (1992)
   PUBMED   1730053
REFERENCE   9  (bases 1 to 5487)
  AUTHORS   Floy,K.M., Hess,R.O. and Meisner,L.F.
  TITLE     DNA polymerase alpha defect in the N syndrome
  JOURNAL   Am J Med Genet 35 (3), 301-305 (1990)
   PUBMED   1689958
REFERENCE   10 (bases 1 to 5487)
  AUTHORS   Fisher,P.A. and Korn,D.
  TITLE     DNA polymerase-alpha. Purification and structural characterization
            of the near homogeneous enzyme from human KB cells
  JOURNAL   J Biol Chem 252 (18), 6528-6535 (1977)
   PUBMED   893425
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC004655.1, AC079375.24,
            KF459037.1, AC135280.2 and AC002504.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001330360.1.
            
            Summary: This gene encodes the catalytic subunit of DNA polymerase,
            which together with a regulatory and two primase subunits, forms
            the DNA polymerase alpha complex. The catalytic subunit plays an
            essential role in the initiation of DNA replication. [provided by
            RefSeq, Mar 2010].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.189376.1,
                                           SRR1803613.231149.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000379068.8/ ENSP00000368358.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-87                AC004655.1         97677-97763
            88-212              AC004655.1         103184-103308
            213-309             AC004655.1         107010-107106
            310-390             AC004655.1         108148-108228
            391-506             AC004655.1         118313-118428
            507-569             AC004655.1         118900-118962
            570-662             AC004655.1         120121-120213
            663-750             AC004655.1         120643-120730
            751-952             AC004655.1         121049-121250
            953-1131            AC004655.1         121339-121517
            1132-1244           AC004655.1         126914-127026
            1245-1361           AC004655.1         128094-128210
            1362-1436           AC004655.1         129740-129814
            1437-1575           AC004655.1         130692-130830
            1576-1730           AC004655.1         131541-131695
            1731-1815           AC079375.24        169365-169449       c
            1816-1877           KF459037.1         112-173
            1878-1967           AC079375.24        166331-166420       c
            1968-2084           AC079375.24        164078-164194       c
            2085-2260           AC079375.24        162269-162444       c
            2261-2390           AC079375.24        160315-160444       c
            2391-2510           AC079375.24        159698-159817       c
            2511-2610           AC079375.24        158490-158589       c
            2611-2735           AC079375.24        156277-156401       c
            2736-2885           AC079375.24        153359-153508       c
            2886-3008           AC079375.24        152827-152949       c
            3009-3041           AC079375.24        91889-91921         c
            3042-3134           AC079375.24        91019-91111         c
            3135-3340           AC079375.24        88956-89161         c
            3341-3473           AC079375.24        86708-86840         c
            3474-3605           AC079375.24        80236-80367         c
            3606-3780           AC079375.24        75218-75392         c
            3781-3959           AC079375.24        59989-60167         c
            3960-4091           AC079375.24        58142-58273         c
            4092-4208           AC079375.24        13697-13813         c
            4209-4305           AC135280.2         7373-7469           c
            4306-5487           AC002504.1         69025-70206         c
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xp22.11-p21.3"
     gene            1..5487
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /note="DNA polymerase alpha 1, catalytic subunit"
                     /db_xref="GeneID:5422"
                     /db_xref="HGNC:HGNC:9173"
                     /db_xref="MIM:312040"
     exon            1..87
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     CDS             45..4451
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /EC_number="2.7.7.7"
                     /note="isoform 1 is encoded by transcript variant 1;
                     polymerase (DNA-directed), alpha (70kD); polymerase (DNA)
                     alpha 1, catalytic subunit; DNA polymerase alpha catalytic
                     subunit p180; polymerase (DNA directed), alpha 1,
                     catalytic subunit"
                     /codon_start=1
                     /product="DNA polymerase alpha catalytic subunit isoform
                     1"
                     /protein_id="NP_001317289.1"
                     /db_xref="CCDS:CCDS83462.1"
                     /db_xref="GeneID:5422"
                     /db_xref="HGNC:HGNC:9173"
                     /db_xref="MIM:312040"
                     /translation="MAPVHGDDCEIGASALSDSGSFVSSRARREKKSKKGRQEALERL
                     KKAKAGEKYKYEVEDFTGVYEEVDEEQYSKLVQARQDDDWIVDDDGIGYVEDGREIFD
                     DDLEDDALDADEKGKDGKARNKDKRNVKKLAVTKPNNIKSMFIACAGKKTADKAVDLS
                     KDGLLGDILQDLNTETPQITPPPVMILKKKRSIGASPNPFSVHTATAVPSGKIASPVS
                     RKEPPLTPVPLKRAEFAGDDVQVESTEEEQESGAMEFEDGDFDEPMEVEEVDLEPMAA
                     KAWDKESEPAEEVKQEADSGKGTVSYLGSFLPDVSCWDIDQEGDSSFSVQEVQVDSSH
                     LPLVKGADEEQVFHFYWLDAYEDQYNQPGVVFLFGKVWIESAETHVSCCVMVKNIERT
                     LYFLPREMKIDLNTGKETGTPISMKDVYEEFDEKIATKYKIMKFKSKPVEKNYAFEIP
                     DVPEKSEYLEVKYSAEMPQLPQDLKGETFSHVFGTNTSSLELFLMNRKIKGPCWLEVK
                     SPQLLNQPVSWCKVEAMALKPDLVNVIKDVSPPPLVVMAFSMKTMQNAKNHQNEIIAM
                     AALVHHSFALDKAAPKPPFQSHFCVVSKPKDCIFPYAFKEVIEKKNVKVEVAATERTL
                     LGFFLAKVHKIDPDIIVGHNIYGFELEVLLQRINVCKAPHWSKIGRLKRSNMPKLGGR
                     SGFGERNATCGRMICDVEISAKELIRCKSYHLSELVQQILKTERVVIPMENIQNMYSE
                     SSQLLYLLEHTWKDAKFILQIMCELNVLPLALQITNIAGNIMSRTLMGGRSERNEFLL
                     LHAFYENNYIVPDKQIFRKPQQKLGDEDEEIDGDTNKYKKGRKKAAYAGGLVLDPKVG
                     FYDKFILLLDFNSLYPSIIQEFNICFTTVQRVASEAQKVTEDGEQEQIPELPDPSLEM
                     GILPREIRKLVERRKQVKQLMKQQDLNPDLILQYDIRQKALKLTANSMYGCLGFSYSR
                     FYAKPLAALVTYKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNK
                     VKSEVNKLYKLLEIDIDGVFKSLLLLKKKKYAALVVEPTSDGNYVTKQELKGLDIVRR
                     DWCDLAKDTGNFVIGQILSDQSRDTIVENIQKRLIEIGENVLNGSVPVSQFEINKALT
                     KDPQDYPDKKSLPHVHVALWINSQGGRKVKAGDTVSYVICQDGSNLTASQRAYAPEQL
                     QKQDNLTIDTQYYLAQQIHPVVARICEPIDGIDAVLIATWLGLDPTQFRVHHYHKDEE
                     NDALLGGPAQLTDEEKYRDCERFKCPCPTCGTENIYDNVFDGSGTDMEPSLYRCSNID
                     CKASPLTFTVQLSNKLIMDIRRFIKKYYDGWLICEEPTCRNRTRHLPLQFSRTGPLCP
                     ACMKATLQPEYSDKSLYTQLCFYRYIFDAECALEKLTTDHEKDKLKKQFFTPKVLQDY
                     RKLKNTAEQFLSRSGYSEVNLSKLFAGCAVKS"
     exon            88..212
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            213..309
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            310..390
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            391..506
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            507..569
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            570..662
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            663..750
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            751..952
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            953..1131
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            1132..1244
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1361
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            1362..1436
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            1437..1575
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            1576..1730
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            1731..1815
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            1816..1877
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            1878..1967
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            1968..2084
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            2085..2260
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            2261..2390
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            2391..2510
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            2511..2610
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            2611..2735
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            2736..2885
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            2886..3008
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            3009..3041
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            3042..3134
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            3135..3340
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            3341..3473
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            3474..3605
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            3606..3780
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            3781..3959
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            3960..4091
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            4092..4208
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            4209..4305
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     exon            4306..5487
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5466..5471
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /note="hexamer: AATAAA"
     polyA_site      5487
                     /gene="POLA1"
                     /gene_synonym="NSX; p180; PDR; POLA; VEODS"
                     /note="major polyA site"
ORIGIN      
        1 gccagttttg ggctggttgg cgcggaatcg ggagattcgg gaccatggca cctgtgcacg
       61 gcgacgactg tgagataggg gcgagtgctc tgtcagattc agggagtttt gtatcttctc
      121 gagcccggcg agaaaaaaaa tcaaagaagg ggcgccaaga agccctagaa agactgaaaa
      181 aggctaaagc tggtgagaag tataaatatg aagtcgagga cttcacaggt gtttatgaag
      241 aagttgatga agaacagtat tcgaagctgg ttcaggcacg ccaggatgat gactggattg
      301 tggatgatga tggtattggc tatgtggaag atggccgaga gatttttgat gatgaccttg
      361 aagatgatgc ccttgatgct gatgagaaag gaaaagatgg taaagcacgc aataaagaca
      421 agaggaatgt aaagaagctc gcagtgacaa aaccgaacaa cattaagtca atgttcattg
      481 cttgtgctgg aaagaaaact gcagataaag ctgtagactt gtccaaggat ggtctgctag
      541 gtgacattct acaggatctt aacactgaga cacctcaaat aactccacca cctgtaatga
      601 tactgaagaa gaaaagatcc attggagctt caccgaatcc tttctctgtg cacaccgcca
      661 cggcagttcc ttcaggaaaa attgcttccc ctgtctccag aaaggagcct ccattaactc
      721 ctgttcctct taaacgtgct gaatttgctg gcgatgatgt acaggtcgag agtacagaag
      781 aagagcagga gtcaggggca atggagtttg aagatggtga ctttgatgag cccatggaag
      841 ttgaagaggt ggacctggag cctatggctg ccaaggcttg ggacaaagag agtgagccag
      901 cagaggaagt gaaacaagag gcggattctg ggaaagggac cgtgtcctac ttaggaagtt
      961 ttctcccgga tgtctcttgt tgggacattg atcaagaagg tgatagcagt ttctcagtgc
     1021 aagaagttca agtggattcc agtcacctcc cattggtaaa aggggcagat gaggaacaag
     1081 tattccactt ttattggttg gatgcttatg aggatcagta caaccaacca ggtgtggtat
     1141 ttctgtttgg gaaagtttgg attgaatcag ccgagaccca tgtgagctgt tgtgtcatgg
     1201 tgaaaaatat cgagcgaacg ctttacttcc ttccccgtga aatgaaaatt gatctaaata
     1261 cggggaaaga aacaggaact ccaatttcaa tgaaggatgt ttatgaggaa tttgatgaga
     1321 aaatagcaac aaaatataaa attatgaagt tcaagtctaa gccagtggaa aagaactatg
     1381 cttttgagat acctgatgtt ccagaaaaat ctgagtactt ggaagttaaa tactcggctg
     1441 aaatgccaca gcttcctcaa gatttgaaag gagaaacttt ttctcatgta tttgggacca
     1501 acacatctag cctggaactg ttcttgatga acagaaagat caaaggacct tgttggcttg
     1561 aagtaaaaag tccacagctc ttgaatcagc cagtcagttg gtgtaaagtt gaggcaatgg
     1621 ctttgaaacc agacctggtg aatgtaatta aggatgtcag tccaccaccg cttgtcgtga
     1681 tggctttcag catgaagaca atgcagaatg caaagaacca tcaaaatgag attattgcta
     1741 tggcagcttt ggtccatcac agttttgcat tggataaagc agccccaaag cctccctttc
     1801 agtcacactt ctgtgttgtg tctaaaccaa aggactgtat ttttccatat gctttcaaag
     1861 aagtcattga gaaaaagaat gtgaaggttg aggttgctgc aacagaaaga acactgctag
     1921 gttttttcct tgcaaaagtt cacaaaattg atcctgatat cattgtgggt cataatattt
     1981 atgggtttga actggaagta ctactgcaga gaattaatgt gtgcaaagct cctcactggt
     2041 ccaagatagg tcgactgaag cgatccaaca tgccaaagct tgggggccgg agtggatttg
     2101 gtgaaagaaa tgctacctgt ggtcgaatga tctgtgatgt ggaaatttca gcaaaggaat
     2161 tgattcgttg taaaagctac catctgtctg aacttgttca gcagattcta aaaactgaaa
     2221 gggttgtaat cccaatggaa aatatacaaa atatgtacag tgaatcttct caactgttat
     2281 acctgttgga acacacctgg aaagatgcca agttcatttt gcagatcatg tgtgagctaa
     2341 atgttcttcc attagcattg cagatcacta acatcgctgg gaacattatg tccaggacgc
     2401 tgatgggtgg acgatccgag cgtaacgagt tcttgttgct tcatgcattt tacgaaaaca
     2461 actatattgt gcctgacaag cagattttca gaaagcctca gcaaaaactg ggagatgaag
     2521 atgaagaaat tgatggagat accaataaat acaagaaagg acgtaagaaa gcagcttatg
     2581 ctggaggctt ggttttggac cccaaagttg gtttttatga taagttcatt ttgcttctgg
     2641 acttcaacag tctatatcct tccatcattc aggaatttaa catttgtttt acaacagtac
     2701 aaagagttgc ttcagaggca cagaaagtta cagaggatgg agaacaagaa cagatccctg
     2761 agttgccaga tccaagctta gaaatgggca ttttgcccag agagatccgg aaactggtag
     2821 aacggagaaa acaagtcaaa cagctaatga aacagcaaga cttaaatcca gaccttattc
     2881 ttcagtatga cattcgacag aaggctttga agctcacagc gaacagtatg tatggttgcc
     2941 tgggattttc ctatagcaga ttttacgcca aaccactggc tgccttggtg acatacaaag
     3001 gaagggagat tttgatgcat acgaaagaga tggtacaaaa gatgaatctt gaagttattt
     3061 atggagatac agattcaatt atgataaaca ccaatagcac caatctggaa gaagtattta
     3121 agttgggaaa caaggtaaaa agtgaagtga ataagttgta caaactgctt gaaatagaca
     3181 ttgatggggt tttcaagtct ctgctactgc tgaaaaaaaa gaagtacgct gctctggttg
     3241 ttgagccaac gtcggatggg aattatgtca ccaaacagga gctcaaagga ttagatatag
     3301 ttagaagaga ttggtgtgat cttgctaaag acactggaaa ctttgtgatt ggccagattc
     3361 tttctgatca aagccgggac actatagtgg aaaacattca gaagaggctg atagaaattg
     3421 gagaaaatgt gctaaatggc agtgtcccag tgagccagtt tgaaattaac aaggcattga
     3481 caaaggatcc ccaggattac cctgataaaa aaagcctacc tcatgtacat gttgccctct
     3541 ggataaattc tcaaggaggc agaaaggtga aagctggaga tactgtgtca tatgtcatct
     3601 gtcaggatgg atcaaacctc actgcaagtc agagggccta tgcgcctgag cagctgcaga
     3661 aacaggataa tctaaccatt gacacccagt actacctggc ccagcagatc cacccagtcg
     3721 tggctcggat ctgtgaacca atagacggaa ttgatgctgt cctcattgca acgtggttgg
     3781 gacttgaccc cacccaattt agagttcatc attatcataa agatgaagag aatgatgctc
     3841 tacttggtgg cccagcacag ctcactgatg aagagaaata cagggactgt gaaagattca
     3901 aatgtccatg ccctacatgt ggaactgaga atatttatga taatgtcttt gatggttcgg
     3961 gaacagatat ggagcccagc ttgtatcgtt gcagtaacat cgattgtaag gcttcacctc
     4021 tgacctttac agtacaactg agcaacaaat tgatcatgga cattagacgt ttcattaaaa
     4081 agtactatga tggctggttg atatgtgaag agccaacctg tcgcaatcga actcgtcacc
     4141 ttccccttca attctcccga actgggcctc tttgcccagc ctgcatgaaa gctacacttc
     4201 aaccagagta ttctgacaag tccctgtaca cccagctgtg cttttaccgg tacatttttg
     4261 atgcggagtg tgcactggag aaacttacta ccgatcatga gaaagataaa ttgaagaagc
     4321 aattttttac ccccaaagtt ctgcaggact acagaaaact caagaacaca gcagagcaat
     4381 tcttgtcccg aagtggctac tccgaagtga atctgagcaa actcttcgct ggttgtgccg
     4441 tgaaatccta agggaatccc aggagtaacc aaggaggggg tagttgaaaa atcccagctt
     4501 cctctgtgcc tccactctgg ccctaaatgc tcctccagca tctgtttctc ccttgggact
     4561 gtgtctcatg tttgtgtgaa tgtagaccag gaaagggggc tgcaaaaatg ttgagtctaa
     4621 tgttcgtaag catcatagaa attcctgtct tcatattaag atgtactgct ttaaaacaca
     4681 actccagagc ccctccccaa gctcccctcc ccaagctcct gaagacccgg tttctgaggg
     4741 agggaaattg ctacttggat tgagagtagc tggaatgtaa gtgaccccag gctttgcctc
     4801 agggccttta gcctatgtcc cccccacata aagagagctt ctcagagcct gactgaagag
     4861 ctgacgtttt gctttttcat atgccaatta aacccggtct aaatccaaat gcttctccag
     4921 ccatccagga gtggctgtcc ttttcagtct tgtcttttat ataggtagct gagggggaag
     4981 atttagaagc cttgcactca ctaaatagat taaacagagc aggcttgttt gttgaattgc
     5041 tccaaagtcc aacagacaca cactgagcag gtgttttaca ctcacattcc ctttttgccc
     5101 cttaaataga aagtgcaggt aaaggtttat acaacaagaa agcacattga aaataatttg
     5161 atactctaac aatccattaa catgtgtagg ggttacggtg aggatcactg tgttgtattc
     5221 agaaaaacgg ggagagggat gcttaattgg ccctggcgct tgctattttt ttctcatttc
     5281 ttcacaatag gaccgtcttt ggcagcagca aaatgtattt cagtatggca gtctttcctc
     5341 tcttacatta ttggtaagat tatactaaca aaatgtttcc ccttgtacaa ttatgctgtg
     5401 tttttaaaaa acattgacct gtgtgttttt ataaaagaaa aagtatgttg tgccttcttc
     5461 ttaagaataa agttttctaa agggaaa
//
