LOCUS       NM_015352               5226 bp    mRNA    linear   PRI 23-JUL-2024
DEFINITION  Homo sapiens protein O-fucosyltransferase 1 (POFUT1), transcript
            variant 1, mRNA.
ACCESSION   NM_015352
VERSION     NM_015352.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Matsumoto,K., Luther,K.B. and Haltiwanger,R.S.
  TITLE     Analysis of endogenous NOTCH1 from POFUT1 S162L patient fibroblasts
            reveals the importance of the O-fucose modification on EGF12 in
            human development
  JOURNAL   Glycobiology 34 (8) (2024)
   PUBMED   38976017
  REMARK    GeneRIF: Analysis of endogenous NOTCH1 from POFUT1 S162L patient
            fibroblasts reveals the importance of the O-fucose modification on
            EGF12 in human development.
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Monno,M., Ogiri,M., Seishima,R., Suzuki,Y., Hattori,K., Matsui,S.,
            Shigeta,K., Okabayashi,K. and Kitagawa,Y.
  TITLE     POFUT1 and PLAGL2 are characteristic markers of mucinous colorectal
            cancer associated with MUC2 expression
  JOURNAL   Cell Biochem Funct 42 (2), e3989 (2024)
   PUBMED   38500386
  REMARK    GeneRIF: POFUT1 and PLAGL2 are characteristic markers of mucinous
            colorectal cancer associated with MUC2 expression.
REFERENCE   3  (bases 1 to 5226)
  AUTHORS   Barlak,N., Kusdemir,G., Gumus,R., Gundogdu,B., Sahin,M.H.,
            Tatar,A., Ittmann,M. and Karatas,O.F.
  TITLE     Overexpression of POFUT1 promotes malignant phenotype and mediates
            perineural invasion in head and neck squamous cell carcinoma
  JOURNAL   Cell Biol Int 47 (12), 1950-1963 (2023)
   PUBMED   37641160
  REMARK    GeneRIF: Overexpression of POFUT1 promotes malignant phenotype and
            mediates perineural invasion in head and neck squamous cell
            carcinoma.
REFERENCE   4  (bases 1 to 5226)
  AUTHORS   Zhang,N., Long,L., Li,G., Wu,X., Peng,S., Jiang,Y., Xiang,A.,
            Mao,X., Huang,H. and Yang,Z.
  TITLE     Preliminary study on the mechanism of POFUT1 in colorectal cancer
  JOURNAL   Med Oncol 40 (8), 235 (2023)
   PUBMED   37432515
  REMARK    GeneRIF: Preliminary study on the mechanism of POFUT1 in colorectal
            cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5226)
  AUTHORS   Luo,M., Deng,X., Chen,Z. and Hu,Y.
  TITLE     Circular RNA circPOFUT1 enhances malignant phenotypes and
            autophagy-associated chemoresistance via sequestrating miR-488-3p
            to activate the PLAG1-ATG12 axis in gastric cancer
  JOURNAL   Cell Death Dis 14 (1), 10 (2023)
   PUBMED   36624091
  REMARK    GeneRIF: Circular RNA circPOFUT1 enhances malignant phenotypes and
            autophagy-associated chemoresistance via sequestrating miR-488-3p
            to activate the PLAG1-ATG12 axis in gastric cancer.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5226)
  AUTHORS   Roos,C., Kolmer,M., Mattila,P. and Renkonen,R.
  TITLE     Composition of Drosophila melanogaster proteome involved in
            fucosylated glycan metabolism
  JOURNAL   J Biol Chem 277 (5), 3168-3175 (2002)
   PUBMED   11698403
REFERENCE   7  (bases 1 to 5226)
  AUTHORS   Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
            Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
            Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
            Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
            Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
            Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
            Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
            Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
            Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
            Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
            Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
            Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
            Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
            Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
            Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
            McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
            Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
            Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
            Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
            Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
            Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
            Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
            Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
            Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
            Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
            Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
REFERENCE   8  (bases 1 to 5226)
  AUTHORS   Wang,Y., Shao,L., Shi,S., Harris,R.J., Spellman,M.W., Stanley,P.
            and Haltiwanger,R.S.
  TITLE     Modification of epidermal growth factor-like repeats with O-fucose.
            Molecular cloning and expression of a novel GDP-fucose protein
            O-fucosyltransferase
  JOURNAL   J Biol Chem 276 (43), 40338-40345 (2001)
   PUBMED   11524432
REFERENCE   9  (bases 1 to 5226)
  AUTHORS   Wang,Y. and Spellman,M.W.
  TITLE     Purification and characterization of a GDP-fucose:polypeptide
            fucosyltransferase from Chinese hamster ovary cells
  JOURNAL   J Biol Chem 273 (14), 8112-8118 (1998)
   PUBMED   9525914
REFERENCE   10 (bases 1 to 5226)
  AUTHORS   Wang,Y., Lee,G.F., Kelley,R.F. and Spellman,M.W.
  TITLE     Identification of a GDP-L-fucose:polypeptide fucosyltransferase and
            enzymatic addition of O-linked fucose to EGF domains
  JOURNAL   Glycobiology 6 (8), 837-842 (1996)
   PUBMED   9023546
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL121897.32.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 25, 2018 this sequence version replaced NM_015352.1.
            
            Summary: This gene encodes a member of the glycosyltransferase
            O-Fuc family. This enzyme adds O-fucose through an O-glycosidic
            linkage to conserved serine or threonine residues in the epidermal
            growth factor-like repeats of a number of cell surface and secreted
            proteins. O-fucose glycans are involved in ligand-induced receptor
            signaling. Alternative splicing of this gene results in two
            transcript variants encoding different isoforms. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes the longer isoform (1)
            of this protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF375884.1, D80002.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000375749.8/ ENSP00000364902.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-186               AL121897.32        20846-21031
            187-308             AL121897.32        23037-23158
            309-491             AL121897.32        28235-28417
            492-604             AL121897.32        29575-29687
            605-797             AL121897.32        41229-41421
            798-1040            AL121897.32        43785-44027
            1041-5226           AL121897.32        47439-51624
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q11.21"
     gene            1..5226
                     /gene="POFUT1"
                     /gene_synonym="DDD2; FUT12; O-Fuc-T; O-FucT-1; O-FUT;
                     OFUCT1"
                     /note="protein O-fucosyltransferase 1"
                     /db_xref="GeneID:23509"
                     /db_xref="HGNC:HGNC:14988"
                     /db_xref="MIM:607491"
     exon            1..186
                     /gene="POFUT1"
                     /gene_synonym="DDD2; FUT12; O-Fuc-T; O-FucT-1; O-FUT;
                     OFUCT1"
                     /inference="alignment:Splign:2.1.0"
     CDS             63..1229
                     /gene="POFUT1"
                     /gene_synonym="DDD2; FUT12; O-Fuc-T; O-FucT-1; O-FUT;
                     OFUCT1"
                     /EC_number="2.4.1.221"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; o-fucosyltransferase protein; GDP-fucose
                     protein O-fucosyltransferase 1;
                     peptide-O-fucosyltransferase 1"
                     /codon_start=1
                     /product="GDP-fucose protein O-fucosyltransferase 1
                     isoform 1 precursor"
                     /protein_id="NP_056167.1"
                     /db_xref="CCDS:CCDS13198.1"
                     /db_xref="GeneID:23509"
                     /db_xref="HGNC:HGNC:14988"
                     /db_xref="MIM:607491"
                     /translation="MGAAAWARPLSVSFLLLLLPLPGMPAGSWDPAGYLLYCPCMGRF
                     GNQADHFLGSLAFAKLLNRTLAVPPWIEYQHHKPPFTNLHVSYQKYFKLEPLQAYHRV
                     ISLEDFMEKLAPTHWPPEKRVAYCFEVAAQRSPDKKTCPMKEGNPFGPFWDQFHVSFN
                     KSELFTGISFSASYREQWSQRFSPKEHPVLALPGAPAQFPVLEEHRPLQKYMVWSDEM
                     VKTGEAQIHAHLVRPYVGIHLRIGSDWKNACAMLKDGTAGSHFMASPQCVGYSRSTAA
                     PLTMTMCLPDLKEIQRAVKLWVRSLDAQSVYVATDSESYVPELQQLFKGKVKVVSLKP
                     EVAQVDLYILGQADHFIGNCVSSFTAFVKRERDLQGRPSSFFGMDRPPKLRDEF"
     exon            187..308
                     /gene="POFUT1"
                     /gene_synonym="DDD2; FUT12; O-Fuc-T; O-FucT-1; O-FUT;
                     OFUCT1"
                     /inference="alignment:Splign:2.1.0"
     exon            309..491
                     /gene="POFUT1"
                     /gene_synonym="DDD2; FUT12; O-Fuc-T; O-FucT-1; O-FUT;
                     OFUCT1"
                     /inference="alignment:Splign:2.1.0"
     exon            492..604
                     /gene="POFUT1"
                     /gene_synonym="DDD2; FUT12; O-Fuc-T; O-FucT-1; O-FUT;
                     OFUCT1"
                     /inference="alignment:Splign:2.1.0"
     exon            605..797
                     /gene="POFUT1"
                     /gene_synonym="DDD2; FUT12; O-Fuc-T; O-FucT-1; O-FUT;
                     OFUCT1"
                     /inference="alignment:Splign:2.1.0"
     exon            798..1040
                     /gene="POFUT1"
                     /gene_synonym="DDD2; FUT12; O-Fuc-T; O-FucT-1; O-FUT;
                     OFUCT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..5226
                     /gene="POFUT1"
                     /gene_synonym="DDD2; FUT12; O-Fuc-T; O-FucT-1; O-FUT;
                     OFUCT1"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1861
                     /gene="POFUT1"
                     /gene_synonym="DDD2; FUT12; O-Fuc-T; O-FucT-1; O-FUT;
                     OFUCT1"
                     /note="major polyA site"
     regulatory      4619..4624
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POFUT1"
                     /gene_synonym="DDD2; FUT12; O-Fuc-T; O-FucT-1; O-FUT;
                     OFUCT1"
                     /note="hexamer: TATAAA"
     polyA_site      4639
                     /gene="POFUT1"
                     /gene_synonym="DDD2; FUT12; O-Fuc-T; O-FucT-1; O-FUT;
                     OFUCT1"
                     /note="major polyA site"
     regulatory      5202..5207
                     /regulatory_class="polyA_signal_sequence"
                     /gene="POFUT1"
                     /gene_synonym="DDD2; FUT12; O-Fuc-T; O-FucT-1; O-FUT;
                     OFUCT1"
                     /note="hexamer: AATGAA"
     polyA_site      5226
                     /gene="POFUT1"
                     /gene_synonym="DDD2; FUT12; O-Fuc-T; O-FucT-1; O-FUT;
                     OFUCT1"
                     /note="major polyA site"
ORIGIN      
        1 gctcgcgtcc ctccttccct ccccgactgt gcgccgcggc tggctcgggt tcccgggccg
       61 acatgggcgc cgccgcgtgg gcacggccgc tgagcgtgtc tttcctgctg ctgcttctgc
      121 cgctcccggg gatgcctgcg ggctcctggg acccggccgg ttacctgctc tactgcccct
      181 gcatggggcg ctttgggaac caggccgatc acttcttggg ctctctggca tttgcaaagc
      241 tgctaaaccg taccttggct gtccctcctt ggattgagta ccagcatcac aagcctcctt
      301 tcaccaacct ccatgtgtcc taccagaagt acttcaagct ggagcccctc caggcttacc
      361 atcgggtcat cagcttggag gatttcatgg agaagctggc acccacccac tggccccctg
      421 agaagcgggt ggcatactgc tttgaggtgg cagcccagcg aagcccagat aagaagacgt
      481 gccccatgaa ggaaggaaac ccctttggcc cattctggga tcagtttcat gtgagtttca
      541 acaagtcgga gctttttaca ggcatttcct tcagtgcttc ctacagagaa caatggagcc
      601 agagattttc tccaaaggaa catccggtgc ttgccctgcc aggagcccca gcccagttcc
      661 ccgtcctaga ggaacacagg ccactacaga agtacatggt atggtcagac gaaatggtga
      721 agacgggaga ggcccagatt catgcccacc ttgtccggcc ctatgtgggc attcatctgc
      781 gcattggctc tgactggaag aacgcctgtg ccatgctgaa ggacgggact gcaggctcgc
      841 acttcatggc ctctccgcag tgtgtgggct acagccgcag cacagcggcc cccctcacga
      901 tgactatgtg cctgcctgac ctgaaggaga tccagagggc tgtgaagctc tgggtgaggt
      961 cgctggatgc ccagtcggtc tacgttgcta ctgattccga gagttatgtg cctgagctcc
     1021 aacagctctt caaagggaag gtgaaggtgg tgagcctgaa gcctgaggtg gcccaggtcg
     1081 acctgtacat cctcggccaa gccgaccact ttattggcaa ctgtgtctcc tccttcactg
     1141 cctttgtgaa gcgggagcgg gacctccagg ggaggccgtc ttctttcttc ggcatggaca
     1201 ggccccctaa gctgcgggac gagttctgat tctggccgga gcaccagacc ctctgatcct
     1261 ggagggacca gagtctgagc tggtccttcc agccaggcct ggcagccaga ggtgctccgg
     1321 gattgcaaac tcctcttctc acctgccaaa gatggagaag agtgccaggg acccctcaag
     1381 gagggagacg ctccatatcc cagggcatag gacttgcagg ttcctaggag caggagcatc
     1441 tcccatcgca cgtgctttct gctcttctgg gaatttctca cactggcaaa gcagtccagc
     1501 ctccgtcttc tggtccactc tgctctgagc agcctgggat gctgaactct tcagagagat
     1561 ttttttatag agagatttct ataattttga tacaaggtca tgactatcct agaactctct
     1621 gtggtttttg aaaatcattg aattctatta atgtaggtac ctaaagtgac cttaactgaa
     1681 tgtggatgag gctggggctg gtgtgggtct tttggctgct tttcaaggtg tcccccaatg
     1741 tggccctcaa gagccatccc cactgcctgg ccagagccat tgttgtcccc tacttcctag
     1801 gccatttctg gggcttgggg gatgaatgct gtcctgtgct gtaaacacta tgcaaatgga
     1861 agttatcggt tgtggtgctg tgcagcgctc tgtgggcgac taagtgccac tcacgcagca
     1921 tgttcctggc aaggagcaca taccatcaag ccacactatc atggtattgt tctcacagtc
     1981 ttttggtggt tgatggccac tgcaaacctg gcaccatcag atctcttctg atctcttgcc
     2041 ccagtggggc ctggttggta gaatgttggc attcggttga tatccaaagc ctgttctccc
     2101 agccgtcctc ctgcagctgg agccttcagg ccgtattctc acgagggaac gtttgccaag
     2161 gctctgacct cacagaagat gcccagggcc cagaagccat cagaattatc agtggagaag
     2221 caccttttga ctcttccctt ccaatgtaat ctctgccaac accatgaggc ttaaggtgct
     2281 ctaagtcatg agtgttttgg tctcaaatgc tgcagtttta ataatctgtg actcctgaga
     2341 gcccatggtt ttttgacctt gtggttctaa aattccttgt ctgacccctg tagatctttt
     2401 ccttgccatg tcacctccct tggcctttga tcctggaaag gtggcagagc ctccactgag
     2461 ccaggcccag agctccttgc agtgccttct tccttgttta cctgtgggag gaaacacttt
     2521 ttttgtcagg ggcagcctgg ttcagagctc agaggtcaca ctgtatcaaa gatctcaaac
     2581 agcaaagtca gcatttgctg tatagagctg ccacccaact ctaagcagga gaaactgtac
     2641 agaaagggct ttgctatttt tcccttttgg gaaaacaatg aagtgtttta agtcctgggt
     2701 ggactgagag atggtttgcc tgtccagact tgctctcaag cctcatccag agaaggagct
     2761 gcagatgagg gagcccgtac actccctgcc accactaggt tgtaagcctg tagctggctg
     2821 gctgatttca ttttggaatt catttgccat ccacagcctt acactaggca cacactttag
     2881 agtctggggc tccagtgggg cccgcctaat tttttttccc cccaagacag ggccttgctc
     2941 tgtctcccag gctggagtgc agtggcatga tcatggctta ctgcagcctt gatctcccag
     3001 gctcaagcga tccttctgcc tcagcctctc tggtagctga gactgcatgc ccagctccaa
     3061 atcaccttga ttcatatcag cagtaataat cacttgtgtt ctgaaagaaa gggcaccaga
     3121 agttctagca aaattcagtt gtgttctgtg agctagcact ttttcctctg acccaatttt
     3181 cttacctata aaatggtgat aaaaaccgac aggttgttca aaggcccaga tcagctaaag
     3241 catgtatata agagcacgtt gtaaacttga aagagacaaa ggcacaaatg tggctgttga
     3301 ttaatttgac tgcttctcgt tgctcgtcac ctccatgcca ggcactgtgc ttgctaattg
     3361 ctttatgggg gcattctctt atttattccc cagccctggg aaataggagc tgtcattatc
     3421 cttctctttc tgcacaagga aaaattaatg ccctgagaat tgtcataatt ttcccaaggc
     3481 tgcccagctg gtggtgttaa gccagaattt gacctcccag agccagtttc cattagctgc
     3541 catgctctgc tgcctctaat tcacagaatg cactttctac cctgtgtgcc atggagacct
     3601 cctatggaaa aatgatcagc caccttacct tctactgggt acctgctgtg agtctgccta
     3661 tgccagaagg attaaggagg ggaggttacc caagaaacaa agcctacatg ccgcttacag
     3721 cccccgttgg atggttgctc agtacaacag tcttgcattc agcaggtgtt tgttcatcac
     3781 ctactatgtg tcaggctcta tgctaggtac tggggataca ggagagaatc aagcgtaaag
     3841 tctttgttct caaggaattt gcattctaga aagtagaaga tgtaataaat gtactgtggg
     3901 acatgttaat aagtgctata aagaaatata aagggtttgg gagcaaaaag agggagtgga
     3961 tctattttag atgagcccag gtaagacctc tctgaagagc tgtcatgaag gagggaggga
     4021 gcacattcct ggcagagaaa acagcacgtg caaaggcccc gagactggag tgtgttcctg
     4081 aagagcagcc aggaggccag catggctgga gaggcaggca taggcaggga accgagcagc
     4141 aggtcagagc aggcgagctg acattctgca gcctggacgg ccatggcagg aagcttttag
     4201 ttggagagat acaggaagcc tcctagggtt ctgagcagaa gaggggcatg agctgattca
     4261 cattctgaag gacctctcta gctggccagt gctgaggagg ttggagagag aaagggtgaa
     4321 agcagagaga ccagtgcagg gctgttaaca gggttgcagg cgagagactg gggtgctggg
     4381 ctcccctaga ctaggactcc agtgccctcc tctcccaaga gacaaaggcc attgcattga
     4441 aggaggtggg aaatgattag attctgaaca tatgtaatta tttttcagtc tttttcaaag
     4501 atacaaatat ttacatagtt ttaatcatgt aatatataca atttaatgtc ctagtgtttt
     4561 acttaatagt gtatcatgtt ttccctgttg gtatgtagcc tggataaatg ctcttaatta
     4621 taaaaaattc tgtcgaggag tgttccatag tttattgttt tcctattatg agaatttagg
     4681 ccaagtgtgg tggctcatgc ctgtaatccc agcactttgc gaggccgagg tgggcagatc
     4741 acttgaggtg aggagttcaa gaccagcctg gccaacatgg tgaattatct ctactaaaaa
     4801 tacaaaaaaa taataataat agccaggcgt ggtggcacat gcctgtattc ccagctgctt
     4861 gggaggctga ggcaggagaa tggcttgaac ctgggaggtg gaggttgcag tgagccgaga
     4921 tggtgccact gcattccagc ctgggcaaca gagcgagact ccatctcaaa aaaaaggaga
     4981 cttcatgtgc ccccaatttt tcactattgt tatttgaaaa aatattttta tttgtaagag
     5041 tttttcttta tttaaaatgt tcattaataa agttgttgga cgggaagcaa aaaaaaaaag
     5101 ttgtttaaga taaattccca gaagtgaatt tgttagatca aacacttaaa actttttgtt
     5161 atggaagaat tcaaatataa ataaaaaatt gtgagtaata aaatgaactc acagtttcaa
     5221 caatga
//
