LOCUS       NM_033109               4549 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens polyribonucleotide nucleotidyltransferase 1 (PNPT1),
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_033109
VERSION     NM_033109.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Guan,C., Zou,X., Yang,C., Shi,W., Gao,J., Ge,Y., Xu,Z., Bi,S. and
            Zhong,X.
  TITLE     Polyribonucleotide nucleotidyltransferase 1 participates in
            metabolic-associated fatty liver disease pathogenesis by affecting
            lipid metabolism and mitochondrial homeostasis
  JOURNAL   Mol Metab 89, 102022 (2024)
   PUBMED   39218215
  REMARK    GeneRIF: Polyribonucleotide nucleotidyltransferase 1 participates
            in metabolic-associated fatty liver disease pathogenesis by
            affecting lipid metabolism and mitochondrial homeostasis.
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Khoroshkin,M., Buyan,A., Dodel,M., Navickas,A., Yu,J., Trejo,F.,
            Doty,A., Baratam,R., Zhou,S., Lee,S.B., Joshi,T., Garcia,K.,
            Choi,B., Miglani,S., Subramanyam,V., Modi,H., Carpenter,C.,
            Markett,D., Corces,M.R., Mardakheh,F.K., Kulakovskiy,I.V. and
            Goodarzi,H.
  TITLE     Systematic identification of post-transcriptional regulatory
            modules
  JOURNAL   Nat Commun 15 (1), 7872 (2024)
   PUBMED   39251607
  REMARK    Erratum:[Nat Commun. 2024 Oct 28;15(1):9277. doi:
            10.1038/s41467-024-52903-4.. PMID: 39468017]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4549)
  AUTHORS   Kim,S., Tan,S., Ku,J., Widowati,T.A., Ku,D., Lee,K., You,K. and
            Kim,Y.
  TITLE     RNA 5-methylcytosine marks mitochondrial double-stranded RNAs for
            degradation and cytosolic release
  JOURNAL   Mol Cell 84 (15), 2935-2948 (2024)
   PUBMED   39019044
  REMARK    Erratum:[Mol Cell. 2025 Feb 4:S1097-2765(25)00079-6. doi:
            10.1016/j.molcel.2025.01.029.. PMID: 39909040]
REFERENCE   4  (bases 1 to 4549)
  AUTHORS   Vedrenne,V., Gowher,A., De Lonlay,P., Nitschke,P., Serre,V.,
            Boddaert,N., Altuzarra,C., Mager-Heckel,A.M., Chretien,F.,
            Entelis,N., Munnich,A., Tarassov,I. and Rotig,A.
  TITLE     Mutation in PNPT1, which encodes a polyribonucleotide
            nucleotidyltransferase, impairs RNA import into mitochondria and
            causes respiratory-chain deficiency
  JOURNAL   Am J Hum Genet 91 (5), 912-918 (2012)
   PUBMED   23084291
  REMARK    GeneRIF: Mutation in PNPT1, which encodes a polyribonucleotide
            nucleotidyltransferase, impairs RNA import into mitochondria and
            causes respiratory-chain deficiency.
REFERENCE   5  (bases 1 to 4549)
  AUTHORS   von Ameln,S., Wang,G., Boulouiz,R., Rutherford,M.A., Smith,G.M.,
            Li,Y., Pogoda,H.M., Nurnberg,G., Stiller,B., Volk,A.E., Borck,G.,
            Hong,J.S., Goodyear,R.J., Abidi,O., Nurnberg,P., Hofmann,K.,
            Richardson,G.P., Hammerschmidt,M., Moser,T., Wollnik,B.,
            Koehler,C.M., Teitell,M.A., Barakat,A. and Kubisch,C.
  TITLE     A mutation in PNPT1, encoding mitochondrial-RNA-import protein
            PNPase, causes hereditary hearing loss
  JOURNAL   Am J Hum Genet 91 (5), 919-927 (2012)
   PUBMED   23084290
  REMARK    GeneRIF: A mutation in PNPT1, encoding mitochondrial-RNA-import
            protein PNPase, causes hereditary hearing loss.
REFERENCE   6  (bases 1 to 4549)
  AUTHORS   Leszczyniecka,M., Su,Z.Z., Kang,D.C., Sarkar,D. and Fisher,P.B.
  TITLE     Expression regulation and genomic organization of human
            polynucleotide phosphorylase, hPNPase(old-35), a Type I interferon
            inducible early response gene
  JOURNAL   Gene 316, 143-156 (2003)
   PUBMED   14563561
REFERENCE   7  (bases 1 to 4549)
  AUTHORS   Sarkar,D., Leszczyniecka,M., Kang,D.C., Lebedeva,I.V., Valerie,K.,
            Dhar,S., Pandita,T.K. and Fisher,P.B.
  TITLE     Down-regulation of Myc as a potential target for growth arrest
            induced by human polynucleotide phosphorylase (hPNPaseold-35) in
            human melanoma cells
  JOURNAL   J Biol Chem 278 (27), 24542-24551 (2003)
   PUBMED   12721301
  REMARK    GeneRIF: the molecular mechanism of the growth-arresting property
            of hPNPaseold-35
REFERENCE   8  (bases 1 to 4549)
  AUTHORS   Piwowarski,J., Grzechnik,P., Dziembowski,A., Dmochowska,A.,
            Minczuk,M. and Stepien,P.P.
  TITLE     Human polynucleotide phosphorylase, hPNPase, is localized in
            mitochondria
  JOURNAL   J Mol Biol 329 (5), 853-857 (2003)
   PUBMED   12798676
  REMARK    GeneRIF: demonstrate that the hPNPase is localized in mitochondria;
            finding suggests the involvement of mitochondrial RNA metabolism in
            cellular senescence
REFERENCE   9  (bases 1 to 4549)
  AUTHORS   Leszczyniecka,M., Kang,D.C., Sarkar,D., Su,Z.Z., Holmes,M.,
            Valerie,K. and Fisher,P.B.
  TITLE     Identification and cloning of human polynucleotide phosphorylase,
            hPNPase old-35, in the context of terminal differentiation and
            cellular senescence
  JOURNAL   Proc Natl Acad Sci U S A 99 (26), 16636-16641 (2002)
   PUBMED   12473748
  REMARK    GeneRIF: an important role for hPNPase(old-35)in growth control
            associated with terminal differentiation and cellular senescence.
REFERENCE   10 (bases 1 to 4549)
  AUTHORS   Raijmakers,R., Egberts,W.V., van Venrooij,W.J. and Pruijn,G.J.
  TITLE     Protein-protein interactions between human exosome components
            support the assembly of RNase PH-type subunits into a six-membered
            PNPase-like ring
  JOURNAL   J Mol Biol 323 (4), 653-663 (2002)
   PUBMED   12419256
  REMARK    GeneRIF: Protein-protein interactions between human exosome
            components support the assembly of RNase PH-type subunits into a
            six-membered PNPase-like ring
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB461021.1, AJ458465.1,
            BC009057.1, CR749867.1, BU943827.1, AW731791.1 and AC015982.9.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_033109.4.
            
            Summary: The protein encoded by this gene belongs to the
            evolutionary conserved polynucleotide phosphorylase family
            comprised of phosphate dependent 3'-to-5' exoribonucleases
            implicated in RNA processing and degradation. This enzyme is
            predominantly localized in the mitochondrial intermembrane space
            and is involved in import of RNA to mitochondria. Mutations in this
            gene have been associated with combined oxidative phosphorylation
            deficiency-13 and autosomal recessive nonsyndromic deafness-70.
            Related pseudogenes are found on chromosomes 3 and 7. [provided by
            RefSeq, Dec 2012].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853563.18175.1,
                                           SRR11853564.15405.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: PMID: 12798676; reported by
                                                  MitoCarta
            MANE Ensembl match                 :: ENST00000447944.7/
                                                  ENSP00000400646.2
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 DB461021.1         67-69
            4-381               AJ458465.1         1-378
            382-382             BC009057.1         364-364
            383-2619            AJ458465.1         380-2616
            2620-4048           CR749867.1         988-2416
            4049-4452           BU943827.1         127-530
            4453-4547           AW731791.1         10-104              c
            4548-4549           AC015982.9         113958-113959       c
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p16.1"
     gene            1..4549
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /note="polyribonucleotide nucleotidyltransferase 1"
                     /db_xref="GeneID:87178"
                     /db_xref="HGNC:HGNC:23166"
                     /db_xref="MIM:610316"
     exon            1..182
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     CDS             22..2373
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /EC_number="2.7.7.8"
                     /note="polyribonucleotide nucleotidyltransferase 1,
                     mitochondrial; PNPase 1; PNPase old-35; 3'-5' RNA
                     exonuclease OLD35; polynucleotide phosphorylase 1;
                     polynucleotide phosphorylase-like protein"
                     /codon_start=1
                     /product="polyribonucleotide nucleotidyltransferase 1,
                     mitochondrial precursor"
                     /protein_id="NP_149100.2"
                     /db_xref="CCDS:CCDS1856.1"
                     /db_xref="GeneID:87178"
                     /db_xref="HGNC:HGNC:23166"
                     /db_xref="MIM:610316"
                     /translation="MAACRYCCSCLRLRPLSDGPFLLPRRDRALTQLQVRALWSSAGS
                     RAVAVDLGNRKLEISSGKLARFADGSAVVQSGDTAVMVTAVSKTKPSPSQFMPLVVDY
                     RQKAAAAGRIPTNYLRREIGTSDKEILTSRIIDRSIRPLFPAGYFYDTQVLCNLLAVD
                     GVNEPDVLAINGASVALSLSDIPWNGPVGAVRIGIIDGEYVVNPTRKEMSSSTLNLVV
                     AGAPKSQIVMLEASAENILQQDFCHAIKVGVKYTQQIIQGIQQLVKETGVTKRTPQKL
                     FTPSPEIVKYTHKLAMERLYAVFTDYEHDKVSRDEAVNKIRLDTEEQLKEKFPEADPY
                     EIIESFNVVAKEVFRSIVLNEYKRCDGRDLTSLRNVSCEVDMFKTLHGSALFQRGQTQ
                     VLCTVTFDSLESGIKSDQVITAINGIKDKNFMLHYEFPPYATNEIGKVTGLNRRELGH
                     GALAEKALYPVIPRDFPFTIRVTSEVLESNGSSSMASACGGSLALMDSGVPISSAVAG
                     VAIGLVTKTDPEKGEIEDYRLLTDILGIEDYNGDMDFKIAGTNKGITALQADIKLPGI
                     PIKIVMEAIQQASVAKKEILQIMNKTISKPRASRKENGPVVETVQVPLSKRAKFVGPG
                     GYNLKKLQAETGVTISQVDEETFSVFAPTPSAMHEARDFITEICKDDQEQQLEFGAVY
                     TATITEIRDTGVMVKLYPNMTAVLLHNTQLDQRKIKHPTALGLEVGQEIQVKYFGRDP
                     ADGRMRLSRKVLQSPATTVVRTLNDRSSIVMGEPISQSSSNSQ"
     exon            183..243
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     exon            244..318
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     exon            319..424
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     exon            425..474
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     exon            475..538
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     exon            539..586
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     exon            587..700
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     exon            701..887
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     exon            888..939
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     exon            940..997
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     exon            998..1094
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1197
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     exon            1198..1268
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     exon            1269..1305
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     exon            1306..1372
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     exon            1373..1462
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     exon            1463..1516
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     exon            1517..1623
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     exon            1624..1695
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     exon            1696..1759
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     exon            1760..1843
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     exon            1844..1927
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     exon            1928..2034
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     exon            2035..2090
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     exon            2091..2169
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     exon            2170..2217
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     exon            2218..4549
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2480..2485
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /note="hexamer: AATACA"
     polyA_site      2506
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /note="major polyA site"
     regulatory      2593..2598
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /note="hexamer: ATTAAA"
     polyA_site      2627
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /note="major polyA site"
     regulatory      3492..3497
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /note="hexamer: AGTAAA"
     polyA_site      3513
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
     regulatory      4497..4502
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /note="hexamer: ATTAAA"
     polyA_site      4520
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
     regulatory      4522..4527
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
                     /note="hexamer: ATTAAA"
     polyA_site      4549
                     /gene="PNPT1"
                     /gene_synonym="COXPD13; DFNB70; old-35; OLD35; PNPASE;
                     SCA25"
ORIGIN      
        1 gttgaccgcg tgccgggtgt catggcggcc tgcaggtact gctgctcgtg cctccggctc
       61 cggcccctga gcgatggtcc tttccttctg ccacggcggg atcgggcact cacccagttg
      121 caagtgcgag cactatggag tagcgcaggg tctcgagctg tggccgtgga cttaggcaac
      181 aggaaattag aaatatcttc tggaaagctg gccagatttg cagatggctc tgctgtagta
      241 cagtcaggtg acactgcagt aatggtcaca gcggtcagta aaacaaaacc ttccccttcc
      301 cagtttatgc ctttggtggt tgactacaga caaaaagctg ctgcagcagg tagaattccc
      361 acaaactatc tgagaagaga gattggtact tctgataaag aaattctaac aagtcgaata
      421 atagatcgtt caattagacc gctctttcca gctggctact tctatgatac acaggttctg
      481 tgtaatctgt tagcagtaga tggtgtaaat gagcctgatg tcctagcaat taatggcgct
      541 tccgtagccc tctcattatc agatattcct tggaatggac ctgttggggc agtacgaata
      601 ggaataattg atggagaata tgttgttaac ccaacaagaa aagaaatgtc ttctagtact
      661 ttaaatttag tggttgctgg agcacctaaa agtcagattg tcatgttgga agcctctgca
      721 gagaacattt tacagcagga cttttgccat gctatcaaag tgggagtgaa atatacccaa
      781 caaataattc agggcattca gcagttggta aaagaaactg gtgttaccaa gaggacacct
      841 cagaagttat ttaccccttc gccagagatt gtgaaatata ctcataaact tgctatggag
      901 agactctatg cagtttttac agattacgag catgacaaag tttccagaga tgaagctgtt
      961 aacaaaataa gattagatac ggaggaacaa ctaaaagaaa aatttccaga agccgatcca
     1021 tatgaaataa tagaatcctt caatgttgtt gcaaaggaag tttttagaag tattgttttg
     1081 aatgaataca aaaggtgcga tggtcgggat ttgacttcac ttaggaatgt aagttgtgag
     1141 gtagatatgt ttaaaaccct tcatggatca gcattatttc aaagaggaca aacacaggtg
     1201 ctttgtaccg ttacatttga ttcattagaa tctggtatta agtcagatca agttataaca
     1261 gctataaatg ggataaaaga taaaaatttc atgctgcact acgagtttcc tccttatgca
     1321 actaatgaaa ttggcaaagt cactggttta aatagaagag aacttgggca tggtgctctt
     1381 gctgagaaag ctttgtatcc tgttattccc cgagattttc ctttcaccat aagagttaca
     1441 tctgaagtcc tagagtcaaa tgggtcatct tctatggcat ctgcatgtgg cggaagttta
     1501 gcattaatgg attcaggggt tccaatttca tctgctgttg caggcgtagc aataggattg
     1561 gtcaccaaaa ccgatcctga gaagggtgaa atagaagatt atcgtttgct gacagatatt
     1621 ttgggaattg aagattacaa tggtgacatg gacttcaaaa tagctggcac taataaagga
     1681 ataactgcat tacaggctga tattaaatta cctggaatac caataaaaat tgtgatggag
     1741 gctattcaac aagcttcagt ggcaaaaaag gagatattac agatcatgaa caaaactatt
     1801 tcaaaacctc gagcatctag aaaagaaaat ggacctgttg tagaaactgt tcaggttcca
     1861 ttatcaaaac gagcaaaatt tgttggacct ggtggctata acttaaaaaa acttcaggct
     1921 gaaacaggtg taactattag tcaggtggat gaagaaacgt tttctgtatt tgcaccaaca
     1981 cccagtgcta tgcatgaggc aagagacttc attactgaaa tctgcaagga tgatcaggag
     2041 cagcaattag aatttggagc agtatatacc gccacaataa ctgaaatcag agatactggt
     2101 gtaatggtaa aattatatcc aaatatgact gcggtactgc ttcataacac acaacttgat
     2161 caacgaaaga ttaaacatcc tactgcccta ggattagaag ttggccaaga aattcaggtg
     2221 aaatactttg gacgtgaccc agccgatgga agaatgaggc tttctcgaaa agtgcttcag
     2281 tcgccagcta caaccgtggt cagaactttg aatgacagaa gtagtattgt aatgggagaa
     2341 cctatttcac agtcatcatc taattctcag tgattttttt ttttaaagag aattctagaa
     2401 ttctattttg tctagggtga tgtgctgtag agcaacattt tagtagtatc ttccattgtg
     2461 tagatttcta tataatataa atacatttta attatttgta ctaaaatgct catttacatg
     2521 tgccattttt ttaattcgag taacccatat ttgtttaatt gtatttacat tataaatcaa
     2581 gaaatattta ttattaaaag taagtcattt atacatctta gaaaaaatta catagttttg
     2641 tttttacaat tctgaaatat atgaaaaact tagatagaat atgtcatatg ttattataac
     2701 agatctcttc ctacatctta tttttcttct ttgtatacag tagtgacagt ttaccttcag
     2761 tcatcctaca ggtcacctct gtgcccaaca actttacttg cctacaacgg ttcacaaact
     2821 ggaacaggcc atattcaaag ccagtgccta tttcttcaga actgttaaca gatatagtga
     2881 gttgagggag ctaatctgat acacttttga taatataatg cctttcaaat tagttaccaa
     2941 atcataaaca gagtggaata aatataaatg agattctaac taggatgaat gtggtagtaa
     3001 tgatgtatat ttttcaaatt tacctaaaca gaaagattat tggctaaggc aggcggatca
     3061 tgagatcagg agatcgagac catcctggct aacacggtga aaccccgtct ctactaaaag
     3121 tgcaaaaatt agccgggcgt ggtggcgcat gcctgtaatc ctagctactc aggaggctga
     3181 ggcagaagaa tcacttcaac ccaggaggtg gaggttgtag tgagccgaga ttgcgccatt
     3241 gcactccagc tcaggcaaca agagcaaaac tccgtctcaa aaaaaaaaaa aaaattgttt
     3301 acttatcaaa ttgaactagg tcaatttgaa tatgtgtatt gctctaatgt atggaagttc
     3361 ttagtataag agctgaagga tcatgacttt cggttaatag atgtttgaaa tagatgttta
     3421 gaggttactg atacacatgt caagttgttc attcccatca tttaaaatat caccttataa
     3481 ttccacagaa aagtaaatgg tatgtggacg tcataattta gaatgtgtaa tcttttttaa
     3541 aatcttataa aaatacactg gactcagtgg tacgtgcctg tagtcccagc tacttggaag
     3601 gctgaagtgg gaaggtcact agagcccaga agttcgacac tgtagtgtgc aacaatcata
     3661 cctgtgaata gctactgtac tccagcctgg gcaacatagc aggactctta tctctaaaaa
     3721 aaattttaaa tcttataaaa atgtaggtat atctggccag gcatggtggc tcacacctgt
     3781 aatcccaaca ctttgggagg tcaaggcggg tagatcacct gaggtcagga gttcgagacc
     3841 agcctggcca acatggtgaa accccatctc tactaaaaat acaaaaatta gctgggtgtg
     3901 gtggcgggcg cctgtaatcc cagctactca ggaggctgag gcaggagaat ctcttgaacc
     3961 tgggaggcag aggtggcagt gagctgagat cgcaccactg cactccagcc tgggcaaaaa
     4021 gagtaaaact ccatctcaaa aaaaaaaatg gtgggcacag aagctcatgc ctgtaattcc
     4081 agcactttgg gaggccgagg cgggtggatt acatgaggtc aggaattcaa gaccagcctg
     4141 gccaacacgg taaaacccca tctctactaa aaatacaaaa attagctggg catggtggca
     4201 ggcacctgta atcctagcta ctcgggaggc tgaggcagga gaatcacttg agcctgggaa
     4261 atggaggttg cagtgagccg agattgcgcc attgcactcc agcctgggcg acagagcaag
     4321 actccgtctc cagaaaaaaa aaaaaaaaag gtaaattcat gactcagggt taactagttc
     4381 ataaaatatt acaaatttat ctggctaaac caaagatttt taagtcaata aacattgatt
     4441 atatatctat tgtgtgtaaa atagcatgtt aggcaatgtt gatatacttc tgaaatatta
     4501 aagattaatt gaaaatgaaa gattaaattt atttgctttc tacacttga
//
