LOCUS       NM_001256007            4569 bp    mRNA    linear   PRI 19-FEB-2025
DEFINITION  Homo sapiens patatin like domain 8, phospholipase A2 (PNPLA8),
            transcript variant 2, mRNA.
ACCESSION   NM_001256007
VERSION     NM_001256007.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Nakamura,Y., Shimada,I.S., Maroofian,R., Falabella,M., Zaki,M.S.,
            Fujimoto,M., Sato,E., Takase,H., Aoki,S., Miyauchi,A.,
            Koshimizu,E., Miyatake,S., Arioka,Y., Honda,M., Higashi,T.,
            Miya,F., Okubo,Y., Ogawa,I., Scardamaglia,A., Miryounesi,M.,
            Alijanpour,S., Ahmadabadi,F., Herkenrath,P., Dafsari,H.S.,
            Velmans,C., Al Balwi,M., Vitobello,A., Denomme-Pichon,A.S.,
            Jeanne,M., Civit,A., Abdel-Hamid,M.S., Naderi,H., Darvish,H.,
            Bakhtiari,S., Kruer,M.C., Carroll,C.J., Ghayoor Karimiani,E.,
            Khailany,R.A., Abdulqadir,T.A., Ozaslan,M., Bauer,P., Zifarelli,G.,
            Seifi,T., Zamani,M., Al Alam,C., Alvi,J.R., Sultan,T.,
            Efthymiou,S., Pope,S.A.S., Haginoya,K., Matsunaga,T., Osaka,H.,
            Matsumoto,N., Ozaki,N., Ohkawa,Y., Oki,S., Tsunoda,T.,
            Pitceathly,R.D.S., Taketomi,Y., Houlden,H., Murakami,M., Kato,Y.
            and Saitoh,S.
  TITLE     Biallelic null variants in PNPLA8 cause microcephaly by reducing
            the number of basal radial glia
  JOURNAL   Brain 147 (11), 3949-3967 (2024)
   PUBMED   39082157
  REMARK    GeneRIF: Biallelic null variants in PNPLA8 cause microcephaly by
            reducing the number of basal radial glia.
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Tan,Z., Deme,P., Boyapati,K., Claes,B.S.R., Duivenvoorden,A.A.M.,
            Heeren,R.M.A., Tressler,C.M., Haughey,N.J. and Glunde,K.
  TITLE     Key regulator PNPLA8 drives phospholipid reprogramming induced
            proliferation and migration in triple-negative breast cancer
  JOURNAL   Breast Cancer Res 25 (1), 148 (2023)
   PUBMED   38017485
  REMARK    GeneRIF: Key regulator PNPLA8 drives phospholipid reprogramming
            induced proliferation and migration in triple-negative breast
            cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4569)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   4  (bases 1 to 4569)
  AUTHORS   Liu,X., Sims,H.F., Jenkins,C.M., Guan,S., Dilthey,B.G. and
            Gross,R.W.
  TITLE     12-LOX catalyzes the oxidation of 2-arachidonoyl-lysolipids in
            platelets generating eicosanoid-lysolipids that are attenuated by
            iPLA2gamma knockout
  JOURNAL   J Biol Chem 295 (16), 5307-5320 (2020)
   PUBMED   32161117
  REMARK    GeneRIF: 12-LOX catalyzes the oxidation of
            2-arachidonoyl-lysolipids in platelets generating
            eicosanoid-lysolipids that are attenuated by iPLA2gamma knockout.
REFERENCE   5  (bases 1 to 4569)
  AUTHORS   Shukla,A., Saneto,R.P., Hebbar,M., Mirzaa,G. and Girisha,K.M.
  TITLE     A neurodegenerative mitochondrial disease phenotype due to
            biallelic loss-of-function variants in PNPLA8 encoding
            calcium-independent phospholipase A2gamma
  JOURNAL   Am J Med Genet A 176 (5), 1232-1237 (2018)
   PUBMED   29681094
  REMARK    GeneRIF: we report two unrelated individuals with variable but
            similar clinical features of microcephaly, severe global
            developmental delay, spasticity, lactic acidosis, and progressive
            cerebellar atrophy with biallelic loss-of-function variants in
            PNPLA8.
REFERENCE   6  (bases 1 to 4569)
  AUTHORS   Yan,W., Jenkins,C.M., Han,X., Mancuso,D.J., Sims,H.F., Yang,K. and
            Gross,R.W.
  TITLE     The highly selective production of 2-arachidonoyl
            lysophosphatidylcholine catalyzed by purified calcium-independent
            phospholipase A2gamma: identification of a novel enzymatic mediator
            for the generation of a key branch point intermediate in eicosanoid
            signaling
  JOURNAL   J Biol Chem 280 (29), 26669-26679 (2005)
   PUBMED   15908428
REFERENCE   7  (bases 1 to 4569)
  AUTHORS   Murakami,M., Masuda,S., Ueda-Semmyo,K., Yoda,E., Kuwata,H.,
            Takanezawa,Y., Aoki,J., Arai,H., Sumimoto,H., Ishikawa,Y.,
            Ishii,T., Nakatani,Y. and Kudo,I.
  TITLE     Group VIB Ca2+-independent phospholipase A2gamma promotes cellular
            membrane hydrolysis and prostaglandin production in a manner
            distinct from other intracellular phospholipases A2
  JOURNAL   J Biol Chem 280 (14), 14028-14041 (2005)
   PUBMED   15695510
  REMARK    GeneRIF: These results suggest distinct roles for iPLA2beta and
            iPLA2gamma in cellular homeostasis and signaling, a functional link
            between peroxisomal AA release and eicosanoid generation, and a
            potential contribution of iPLA2gamma to tumorigenesis.
REFERENCE   8  (bases 1 to 4569)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
REFERENCE   9  (bases 1 to 4569)
  AUTHORS   Tanaka,H., Takeya,R. and Sumimoto,H.
  TITLE     A novel intracellular membrane-bound calcium-independent
            phospholipase A(2)
  JOURNAL   Biochem Biophys Res Commun 272 (2), 320-326 (2000)
   PUBMED   10833412
REFERENCE   10 (bases 1 to 4569)
  AUTHORS   Mancuso,D.J., Jenkins,C.M. and Gross,R.W.
  TITLE     The genomic organization, complete mRNA sequence, cloning, and
            expression of a novel human intracellular membrane-associated
            calcium-independent phospholipase A(2)
  JOURNAL   J Biol Chem 275 (14), 9937-9945 (2000)
   PUBMED   10744668
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC032999.2, AL834147.1,
            DW410822.1 and AC005058.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001256007.2.
            
            Summary: This gene encodes a member of the patatin-like
            phospholipase domain containing protein family. Members of this
            family are phospholipases which catalyze the cleavage of fatty
            acids from membrane phospholipids. The product of this gene is a
            calcium-independent phospholipase. Mutations in this gene have been
            associated with mitochondrial myopathy with lactic acidosis.
            Multiple transcript variants encoding different isoforms have been
            found for this gene. [provided by RefSeq, May 2015].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1, 2 and 3 encode the same protein (isoform
            1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK291755.1, SRR1803614.32019.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000257694.13/ ENSP00000257694.8
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-153               BC032999.2         60-212
            154-3359            BC032999.2         325-3530
            3360-3772           AL834147.1         3064-3476
            3773-3823           DW410822.1         220-270
            3824-4564           AL834147.1         3529-4269
            4565-4569           AC005058.1         77687-77691         c
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q31.1"
     gene            1..4569
                     /gene="PNPLA8"
                     /gene_synonym="IPLA2-2; IPLA2G; iPLA2gamma; MMLA;
                     PNPLA-gamma"
                     /note="patatin like domain 8, phospholipase A2"
                     /db_xref="GeneID:50640"
                     /db_xref="HGNC:HGNC:28900"
                     /db_xref="MIM:612123"
     exon            1..107
                     /gene="PNPLA8"
                     /gene_synonym="IPLA2-2; IPLA2G; iPLA2gamma; MMLA;
                     PNPLA-gamma"
                     /inference="alignment:Splign:2.1.0"
     exon            108..153
                     /gene="PNPLA8"
                     /gene_synonym="IPLA2-2; IPLA2G; iPLA2gamma; MMLA;
                     PNPLA-gamma"
                     /inference="alignment:Splign:2.1.0"
     exon            154..1292
                     /gene="PNPLA8"
                     /gene_synonym="IPLA2-2; IPLA2G; iPLA2gamma; MMLA;
                     PNPLA-gamma"
                     /inference="alignment:Splign:2.1.0"
     CDS             237..2585
                     /gene="PNPLA8"
                     /gene_synonym="IPLA2-2; IPLA2G; iPLA2gamma; MMLA;
                     PNPLA-gamma"
                     /EC_number="3.1.1.5"
                     /note="isoform 1 is encoded by transcript variant 2;
                     intracellular membrane-associated calcium-independent
                     phospholipase A2 gamma; patatin like phospholipase domain
                     containing 8"
                     /codon_start=1
                     /product="calcium-independent phospholipase A2-gamma
                     isoform 1"
                     /protein_id="NP_001242936.1"
                     /db_xref="CCDS:CCDS34733.1"
                     /db_xref="GeneID:50640"
                     /db_xref="HGNC:HGNC:28900"
                     /db_xref="MIM:612123"
                     /translation="MSINLTVDIYIYLLSNARSVCGKQRSKQLYFLFSPKHYWRISHI
                     SLQRGFHTNIIRCKWTKSEAHSCSKHCYSPSNHGLHIGILKLSTSAPKGLTKVNICMS
                     RIKSTLNSVSKAVFGNQNEMISRLAQFKPSSQILRKVSDSGWLKQKNIKQAIKSLKKY
                     SDKSAEKSPFPEEKSHIIDKEEDIGKRSLFHYTSSITTKFGDSFYFLSNHINSYFKRK
                     EKMSQQKENEHFRDKSELEDKKVEEGKLRSPDPGILAYKPGSESVHTVDKPTSPSAIP
                     DVLQVSTKQSIANFLSRPTEGVQALVGGYIGGLVPKLKYDSKSQSEEQEEPAKTDQAV
                     SKDRNAEEKKRLSLQREKIIARVSIDNRTRALVQALRRTTDPKLCITRVEELTFHLLE
                     FPEGKGVAVKERIIPYLLRLRQIKDETLQAAVREILALIGYVDPVKGRGIRILSIDGG
                     GTRGVVALQTLRKLVELTQKPVHQLFDYICGVSTGAILAFMLGLFHMPLDECEELYRK
                     LGSDVFSQNVIVGTVKMSWSHAFYDSQTWENILKDRMGSALMIETARNPTCPKVAAVS
                     TIVNRGITPKAFVFRNYGHFPGINSHYLGGCQYKMWQAIRASSAAPGYFAEYALGNDL
                     HQDGGLLLNNPSALAMHECKCLWPDVPLECIVSLGTGRYESDVRNTVTYTSLKTKLSN
                     VINSATDTEEVHIMLDGLLPPDTYFRFNPVMCENIPLDESRNEKLDQLQLEGLKYIER
                     NEQKMKKVAKILSQEKTTLQKINDWIKLKTDMYEGLPFFSKL"
     exon            1293..1442
                     /gene="PNPLA8"
                     /gene_synonym="IPLA2-2; IPLA2G; iPLA2gamma; MMLA;
                     PNPLA-gamma"
                     /inference="alignment:Splign:2.1.0"
     exon            1443..1594
                     /gene="PNPLA8"
                     /gene_synonym="IPLA2-2; IPLA2G; iPLA2gamma; MMLA;
                     PNPLA-gamma"
                     /inference="alignment:Splign:2.1.0"
     exon            1595..1689
                     /gene="PNPLA8"
                     /gene_synonym="IPLA2-2; IPLA2G; iPLA2gamma; MMLA;
                     PNPLA-gamma"
                     /inference="alignment:Splign:2.1.0"
     exon            1690..1861
                     /gene="PNPLA8"
                     /gene_synonym="IPLA2-2; IPLA2G; iPLA2gamma; MMLA;
                     PNPLA-gamma"
                     /inference="alignment:Splign:2.1.0"
     exon            1862..1919
                     /gene="PNPLA8"
                     /gene_synonym="IPLA2-2; IPLA2G; iPLA2gamma; MMLA;
                     PNPLA-gamma"
                     /inference="alignment:Splign:2.1.0"
     exon            1920..2114
                     /gene="PNPLA8"
                     /gene_synonym="IPLA2-2; IPLA2G; iPLA2gamma; MMLA;
                     PNPLA-gamma"
                     /inference="alignment:Splign:2.1.0"
     exon            2115..2310
                     /gene="PNPLA8"
                     /gene_synonym="IPLA2-2; IPLA2G; iPLA2gamma; MMLA;
                     PNPLA-gamma"
                     /inference="alignment:Splign:2.1.0"
     exon            2311..4569
                     /gene="PNPLA8"
                     /gene_synonym="IPLA2-2; IPLA2G; iPLA2gamma; MMLA;
                     PNPLA-gamma"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3112..3117
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PNPLA8"
                     /gene_synonym="IPLA2-2; IPLA2G; iPLA2gamma; MMLA;
                     PNPLA-gamma"
                     /note="hexamer: ATTAAA"
     polyA_site      3141
                     /gene="PNPLA8"
                     /gene_synonym="IPLA2-2; IPLA2G; iPLA2gamma; MMLA;
                     PNPLA-gamma"
                     /note="major polyA site"
     regulatory      3342..3347
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PNPLA8"
                     /gene_synonym="IPLA2-2; IPLA2G; iPLA2gamma; MMLA;
                     PNPLA-gamma"
                     /note="hexamer: AATAAA"
     regulatory      3355..3360
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PNPLA8"
                     /gene_synonym="IPLA2-2; IPLA2G; iPLA2gamma; MMLA;
                     PNPLA-gamma"
                     /note="hexamer: AATAAT"
     polyA_site      3359
                     /gene="PNPLA8"
                     /gene_synonym="IPLA2-2; IPLA2G; iPLA2gamma; MMLA;
                     PNPLA-gamma"
     polyA_site      3372
                     /gene="PNPLA8"
                     /gene_synonym="IPLA2-2; IPLA2G; iPLA2gamma; MMLA;
                     PNPLA-gamma"
     regulatory      4384..4389
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PNPLA8"
                     /gene_synonym="IPLA2-2; IPLA2G; iPLA2gamma; MMLA;
                     PNPLA-gamma"
                     /note="hexamer: AATAAA"
     polyA_site      4406
                     /gene="PNPLA8"
                     /gene_synonym="IPLA2-2; IPLA2G; iPLA2gamma; MMLA;
                     PNPLA-gamma"
     regulatory      4546..4551
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PNPLA8"
                     /gene_synonym="IPLA2-2; IPLA2G; iPLA2gamma; MMLA;
                     PNPLA-gamma"
                     /note="hexamer: AATACA"
     regulatory      4551..4556
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PNPLA8"
                     /gene_synonym="IPLA2-2; IPLA2G; iPLA2gamma; MMLA;
                     PNPLA-gamma"
                     /note="hexamer: ATTACA"
     polyA_site      4564
                     /gene="PNPLA8"
                     /gene_synonym="IPLA2-2; IPLA2G; iPLA2gamma; MMLA;
                     PNPLA-gamma"
     polyA_site      4569
                     /gene="PNPLA8"
                     /gene_synonym="IPLA2-2; IPLA2G; iPLA2gamma; MMLA;
                     PNPLA-gamma"
ORIGIN      
        1 agtgtttgtg ttggaagctc agctgatgca ggccggttgg agtggacgtc attgccggga
       61 acgagcgagt cgccgctgca gccctagtga ctgcggcctg catcccgatt gtcttctcct
      121 ccaaggtcta catgattacc tgaagtttaa taagtaagac catgaattat ggcatttctt
      181 aaatgaagcg ttcaagaagt gagagaatgt catagaaaat aaatgatttt taagttatgt
      241 ctattaatct gactgtagat atatatattt acctccttag taatgcaaga agtgtttgtg
      301 ggaagcagag aagcaagcaa ctgtatttct tgttctcacc taagcattac tggaggataa
      361 gccacatcag tctacaaaga ggttttcata caaacataat aagatgtaaa tggaccaaaa
      421 gtgaagcaca ttcttgcagt aagcactgtt actctccaag caaccatggt ttacatattg
      481 ggattttgaa acttagcact tctgctccca agggacttac aaaagtgaac atttgtatgt
      541 cccgtattaa aagtactttg aactctgttt caaaggctgt ttttggcaat caaaatgaaa
      601 tgatttcacg tttagctcaa tttaagccaa gttcccaaat tttaagaaaa gtatcggata
      661 gtggctggtt aaaacagaaa aacatcaaac aagccatcaa atctctgaaa aaatatagtg
      721 acaaatcagc agaaaagagt ccttttccag aagagaaaag tcacattata gacaaagaag
      781 aagatatagg taaacgcagt ctttttcatt acacaagttc tataaccaca aaatttggag
      841 actcattcta ctttttatca aatcatatta attcatattt caaacgtaag gaaaaaatgt
      901 ctcaacaaaa ggaaaatgaa catttccggg acaaatcaga acttgaagat aaaaaggtag
      961 aagaggggaa attaagatct ccagatcctg gcatcctggc ttataagcca ggctcagaat
     1021 ctgtacatac ggtggacaag cctacaagtc cttctgcgat acctgatgtt cttcaagttt
     1081 caactaaaca aagtattgct aactttcttt ctcgtcccac ggaaggtgta caagctttag
     1141 taggtggtta tattggtgga cttgtcccca aattaaagta tgattcaaag agtcagtcag
     1201 aagaacagga agagcctgct aaaactgatc aggctgtcag caaagacaga aatgcagagg
     1261 agaaaaagcg tttatctctt cagcgagaaa agattatcgc aagggtgagt attgataaca
     1321 ggacccgggc attagttcag gcattaagaa gaacaactga cccaaagctc tgcattacta
     1381 gggttgaaga actgactttt catcttctag aatttcctga aggaaaagga gtggctgtca
     1441 aggaaagaat tattccatat ttattacgac tgagacaaat taaggatgaa actcttcagg
     1501 ctgcagttag agaaattttg gccctaattg gctatgtgga tccagtgaaa gggagaggaa
     1561 tccgaattct ctcaattgat ggtggaggaa caaggggcgt ggttgctctc cagaccctac
     1621 gaaaattagt tgaacttact cagaagccag ttcatcagct ctttgattac atttgtggtg
     1681 taagcacagg tgccatatta gctttcatgt tggggttgtt tcatatgccc ttggatgaat
     1741 gtgaggaact ttatcgaaaa ttaggatcag atgtattttc acaaaatgtc attgttggaa
     1801 cagtaaaaat gagttggagc catgcatttt atgacagtca aacatgggaa aacattctta
     1861 aggataggat gggatctgca ctgatgattg aaacagcaag aaaccccaca tgtcctaagg
     1921 tagctgctgt aagtaccata gtaaatagag ggataacacc caaagctttt gtgttcagaa
     1981 actatggtca ttttcctgga atcaactctc attatttggg aggctgtcag tataaaatgt
     2041 ggcaggccat tagagcctca tctgctgctc caggctactt tgcagaatat gcattgggaa
     2101 atgatcttca tcaagatgga ggtttgcttc tgaataaccc ttcggcatta gctatgcatg
     2161 agtgtaaatg tctttggcca gatgtgccgt tagagtgcat agtatccctg ggcactggac
     2221 gttatgagag tgatgtgaga aacacggtaa catacacaag cttgaaaact aaactttcta
     2281 atgttatcaa cagtgctaca gatacagaag aagtccatat aatgcttgat ggcctgttac
     2341 ctcctgacac ctattttaga ttcaatcctg taatgtgtga aaacatacct ctagatgaaa
     2401 gtcgaaatga aaagctggat cagctgcagt tggaagggtt gaaatacata gaaagaaatg
     2461 aacaaaaaat gaaaaaagtt gcaaaaatat taagtcaaga aaaaacaact ctgcagaaaa
     2521 ttaatgattg gataaaatta aaaactgata tgtatgaagg acttccattc ttttcaaaat
     2581 tgtgatgagt atatgcttat gttctcataa atgaaggtct gtttagaaga tcaaccacat
     2641 tcaataagga attgtggggt tcgacatgag ttaactttga aatacgtatg aattctggag
     2701 aatcctgaaa aagacggtgc ttcaaccagc ttgcatagca cagagaatat tcttggttac
     2761 agaattcata tgggaactag gcttttaaga tgttaataat tagctaagct ttagtaaccc
     2821 ttactgtgct agtagatttt agtagatatt ggtgttatat tgtttgatgt ttgaaaatat
     2881 attaatatat gtgccgaaca agaaaccgaa agctatattg tactgtgtat ttttacttta
     2941 gtcctcataa tcatgttgaa tttatgtgat cattgatttt atttcatatg gaaaagctaa
     3001 tttcttctta aatttacatt acctaatatt ctcactagct atgttctcca atccacactg
     3061 ccttttattg taatatcatc taaatagatg cagaaaaatg gaattttctc tattaaagta
     3121 ttttacattt gacataaaaa agaaccagat acagttttct attcagatat gtttatttta
     3181 acattgtttg gttaaaaaag gtgaagttcc agtcaaccac tttttacccc tgaaatttca
     3241 agataatgct atattaactt ttccagatct aacactagct tattcttccc tgttataaaa
     3301 tggtttgaac ttactgagga gatattccta tcattaacaa aaataaacta tttaaataat
     3361 ctgttgttaa aaggctaatg tcattttaaa attaattttt gttcataatg tagctcccct
     3421 ttagcctttg aatacacctg gcagcagtat gcttaaaaat aagaagttag ttgcctggcg
     3481 cagtagctca cacctgtaat cccagcactt tgggaggccg aggcaggcag atcacctgac
     3541 gtcaggagtc caagaccagc ctggccaaca cggtgaaacc tcgtctctaa taaaaataca
     3601 aacattagcc cggcatggtg gtgcacacct atagtcccag ctactcggga ggctaaggca
     3661 ggagaatggc ttgaacctga gagacggagg ttgcagtgag ccaaatagcg ccagtgcact
     3721 ccagcctagg cgacacagcg agactccatc tcaaaaaaaa aaaaaaaaaa aagaagaagt
     3781 taggcttgtt ttaccttatt aactcctttg aactgtctgg atttcatgga agttggtaag
     3841 atgcacttac atggaacatt gttcatgcca aacaaactca tctttattgt atctatttgt
     3901 cacataaatc cttacatgga atgttttgat ttcttaagaa aaatgaaaca aaggttagcg
     3961 ccccttgttt tagacataca aactaaagct agaccctgtt gcccagatag tcatattgct
     4021 tctttgcaat atcaattcac agaggctgtg agatgtggtt caaagagtgg agtcgagagt
     4081 ctggatgcta atcctgcctt tgctgcaaga acccttctgt ttgttacctt gaggaaggca
     4141 aatgtctgta ggctttgcat atctcatttg taaaatgagg aaaggtagct gtaaaatttt
     4201 ctaacatccg aattatataa actttagaca tagtataaaa taggaaacta ccaatgattt
     4261 ctaaagataa tatagttctt tcagaccaca aataatacat aataacatgc aactaagaac
     4321 aacttgtaaa tgcaagaaaa aaacaaataa ttggaaatca gaatatttct gggaattctt
     4381 cgtaataaag caaatttcaa gcacaataaa gtaatattta tgcatattta catatttgca
     4441 cctcatcata aggtcactct agtcctaaac tgatgtagtt catctgtttt acagctgaag
     4501 ttaagtatgt cccttatttg tgcttggctt atttctttct taaataatac attacacaag
     4561 tgaactctg
//
