LOCUS       NM_001166114            4372 bp    mRNA    linear   PRI 19-FEB-2025
DEFINITION  Homo sapiens patatin like domain 6, lysophospholipase (PNPLA6),
            transcript variant 5, mRNA.
ACCESSION   NM_001166114
VERSION     NM_001166114.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Liu,J., He,Y., Lwin,C., Han,M., Guan,B., Naik,A., Bender,C.,
            Moore,N., Huryn,L.A., Sergeev,Y.V., Qian,H., Zeng,Y., Dong,L.,
            Liu,P., Lei,J., Haugen,C.J., Prasov,L., Shi,R., Dollfus,H.,
            Aristodemou,P., Laich,Y., Nemeth,A.H., Taylor,J., Downes,S.,
            Krawczynski,M.R., Meunier,I., Strassberg,M., Tenney,J., Gao,J.,
            Shear,M.A., Moore,A.T., Duncan,J.L., Menendez,B., Hull,S.,
            Vincent,A.L., Siskind,C.E., Traboulsi,E.I., Blackstone,C.,
            Sisk,R.A., Miraldi Utz,V., Webster,A.R., Michaelides,M., Arno,G.,
            Synofzik,M. and Hufnagel,R.B.
  TITLE     Neuropathy target esterase activity defines phenotypes among PNPLA6
            disorders
  JOURNAL   Brain 147 (6), 2085-2097 (2024)
   PUBMED   38735647
  REMARK    GeneRIF: Neuropathy target esterase activity defines phenotypes
            among PNPLA6 disorders.
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Liampas,A., Nicolaou,P., Votsi,C., Georghiou,A., Christodoulou,K.,
            Tanteles,G.A. and Pantzaris,M.
  TITLE     Two case reports of a novel missense mutation in the PNPLA6 gene in
            two siblings with chorioretinal dystrophy, hypogonadotropic
            hypogonadism, and cerebellar ataxia
  JOURNAL   Mol Biol Rep 51 (1), 590 (2024)
   PUBMED   38683245
  REMARK    GeneRIF: Two case reports of a novel missense mutation in the
            PNPLA6 gene in two siblings with chorioretinal dystrophy,
            hypogonadotropic hypogonadism, and cerebellar ataxia.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4372)
  AUTHORS   Hou,W.Y., Song,X., Wang,Y., Chang,P., Chen,R. and Wu,Y.J.
  TITLE     The alteration of the expression level of neuropathy target
            esterase in human neuroblastoma SK-N-SH cells disrupts cellular
            phospholipids homeostasis
  JOURNAL   Toxicol In Vitro 86, 105509 (2023)
   PUBMED   36336212
  REMARK    GeneRIF: The alteration of the expression level of neuropathy
            target esterase in human neuroblastoma SK-N-SH cells disrupts
            cellular phospholipids homeostasis.
REFERENCE   4  (bases 1 to 4372)
  AUTHORS   Lush,M.J., Li,Y., Read,D.J., Willis,A.C. and Glynn,P.
  TITLE     Neuropathy target esterase and a homologous Drosophila
            neurodegeneration-associated mutant protein contain a novel domain
            conserved from bacteria to man
  JOURNAL   Biochem J 332 (Pt 1) (Pt 1), 1-4 (1998)
   PUBMED   9576844
REFERENCE   5  (bases 1 to 4372)
  AUTHORS   Synofzik,M., Hufnagel,R.B. and Zuchner,S.
  TITLE     PNPLA6 Disorders
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   25299038
REFERENCE   6  (bases 1 to 4372)
  AUTHORS   Hedera,P.
  TITLE     Hereditary Spastic Paraplegia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301682
REFERENCE   7  (bases 1 to 4372)
  AUTHORS   Perlman,S.
  TITLE     Hereditary Ataxia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301317
REFERENCE   8  (bases 1 to 4372)
  AUTHORS   Maroni,M. and Bleecker,M.L.
  TITLE     Neuropathy target esterase in human lymphocytes and platelets
  JOURNAL   J Appl Toxicol 6 (1), 1-7 (1986)
   PUBMED   3958422
REFERENCE   9  (bases 1 to 4372)
  AUTHORS   Bertoncin,D., Russolo,A., Caroldi,S. and Lotti,M.
  TITLE     Neuropathy target esterase in human lymphocytes
  JOURNAL   Arch Environ Health 40 (3), 139-144 (1985)
   PUBMED   4026382
REFERENCE   10 (bases 1 to 4372)
  AUTHORS   Gurba,P.E. and Richardson,R.J.
  TITLE     Partial characterization of neurotoxic esterase of human placenta
  JOURNAL   Toxicol Lett 15 (1), 13-17 (1983)
   PUBMED   6836585
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA096881.1, BC038229.1 and
            BC051768.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001166114.1.
            
            Summary: This gene encodes a phospholipase that deacetylates
            intracellular phosphatidylcholine to produce glycerophosphocholine.
            It is thought to function in neurite outgrowth and process
            elongation during neuronal differentiation. The protein is anchored
            to the cytoplasmic face of the endoplasmic reticulum in both
            neurons and non-neuronal cells. Mutations in this gene result in
            autosomal recessive spastic paraplegia, and the protein is the
            target for neurodegeneration induced by organophosphorus compounds
            and chemical warfare agents. Multiple transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Oct 2009].
            
            Transcript Variant: This variant (5) represents use of an alternate
            promoter and 5' UTR, uses a downstream start codon, and uses an
            alternate in-frame splice site in the central coding region,
            compared to variant 1. The resulting isoform (d) has a shorter
            N-terminus and lacks one internal residue, compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC038229.1, SRR7346977.1960364.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000600737.6/ ENSP00000473211.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-125               DA096881.1         15-139
            126-181             DA096881.1         141-196
            182-920             BC038229.1         13-751
            921-2185            BC051768.1         921-2185
            2186-4372           BC038229.1         2017-4203
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.2"
     gene            1..4372
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /note="patatin like domain 6, lysophospholipase"
                     /db_xref="GeneID:10908"
                     /db_xref="HGNC:HGNC:16268"
                     /db_xref="MIM:603197"
     exon            1..304
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     CDS             73..4170
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /EC_number="3.1.1.5"
                     /note="isoform d is encoded by transcript variant 5;
                     neuropathy target esterase; patatin-like phospholipase
                     domain-containing protein 6; patatin like phospholipase
                     domain containing 6"
                     /codon_start=1
                     /product="patatin-like phospholipase domain-containing
                     protein 6 isoform d"
                     /protein_id="NP_001159586.1"
                     /db_xref="CCDS:CCDS59343.1"
                     /db_xref="GeneID:10908"
                     /db_xref="HGNC:HGNC:16268"
                     /db_xref="MIM:603197"
                     /translation="MGTSSHGLATNSSGAKVAERDGFQDVLAPGEGSAGRICGAQPVP
                     FVPQVLGVMIGAGVAVVVTAVLILLVVRRLRVPKTPAPDGPRYRFRKRDKVLFYGRKI
                     MRKVSQSTSSLVDTSVSATSRPRMRKKLKMLNIAKKILRIQKETPTLQRKEPPPAVLE
                     ADLTEGDLANSHLPSEVLYMLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQ
                     PDASIYVVQDGLLELCLPGPDGKECVVKEVVPGDSVNSLLSILDVITGHQHPQRTVSA
                     RAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTFLALHNYLGLTNELFS
                     HEIQPLRLFPSPGLPTRTSPVRGSKRMVSTSATDEPRETPGRPPDPTGAPLPGPTGDP
                     VKPTSLETPSAPLLSRCVSMPGDISGLQGGPRSDFDMAYERGRISVSLQEEASGGSLA
                     APARTPTQEPREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKQELAKLMR
                     IEDPSLLNSRVLLHHAKAGTIIARQGDQDVSLHFVLWGCLHVYQRMIDKAEDVCLFVA
                     QPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSDFYEIMRAQPSVVLSAAHTVAAR
                     MSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRGSGKKELVGEY
                     GRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQK
                     ILGNLQQLQGPFPGSGLGVPPHSELTNPASNLATVAILPVCAEVPMVAFTLELQHALQ
                     AIGPTLLLNSDIIRARLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDASLTPWTVR
                     CLRQADCILIVGLGDQEPTLGQLEQMLENTAVRALKQLVLLHREEGAGPTRTVEWLNM
                     RSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRADRHSDFSRLARVLTGNTIALV
                     LGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRTKQRAREWA
                     KSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAM
                     RVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIA
                     IDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEV
                     VKSSSYCEYLRPPIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWSRGNVIEKMLTDRRS
                     TDLNESRRADVLAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGP
                     DCSRDEGGSPEGASPSTASEMEEEKSILRQRRCLPQEPPGSATDA"
     exon            305..387
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            388..485
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            486..626
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            627..786
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            787..867
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            868..996
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            997..1077
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            1078..1240
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            1241..1324
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            1325..1434
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            1435..1602
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            1603..1680
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            1681..1886
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            1887..2018
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            2019..2142
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            2143..2256
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            2257..2332
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            2333..2473
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            2474..2537
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            2538..2706
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            2707..2889
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            2890..3008
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            3009..3165
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            3166..3282
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            3283..3352
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            3353..3469
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            3470..3771
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            3772..3888
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            3889..3985
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            3986..4095
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     exon            4096..4372
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4352..4357
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /note="hexamer: AATAAA"
     polyA_site      4372
                     /gene="PNPLA6"
                     /gene_synonym="BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS;
                     SPG39; sws"
                     /note="major polyA site"
ORIGIN      
        1 gcactgcggg ccgccgggcc tcagggaaga gtcgcgcccc cggggaggga gcagcactgg
       61 cccattctgc agatggggac atcgagtcac gggctggcta cgaactcctc gggggcgaag
      121 gtggcggaga gggatgggtt ccaggacgtc ctggcgcccg gggaaggctc ggcgggacgg
      181 atttgcggtg cgcagccagt gccgttcgtc cctcaggtgc ttggcgtgat gatcggggcc
      241 ggagtggcgg tggtggtcac ggccgtgctc atcctcctgg tggtgcggag gctgcgagtg
      301 ccaaaaaccc cagccccgga tggcccccgg tatcggttcc ggaagaggga caaagtgctc
      361 ttctatggcc ggaagattat gcggaaggtg tcacaatcca cctcctccct cgtggatacc
      421 tctgtctccg ccacctcccg gccacgcatg aggaagaaac tgaagatgct caacattgcc
      481 aagaagatcc tgcgcatcca gaaagagacg cccacgctgc agcggaagga gcccccgccc
      541 gcagtgctag aagctgacct gaccgagggc gacctggcta actcccatct gccctctgaa
      601 gtgctttata tgctcaagaa cgtccgggtg ctgggccact tcgagaagcc actcttcctg
      661 gagctctgcc gccacatggt cttccagcgg ctgggccagg gtgactacgt cttccggccg
      721 ggccagccag atgccagcat ctacgtggtg caggacgggc tgctggagct ctgtctgcca
      781 gggcctgacg ggaaggagtg tgtggtgaag gaagtggttc ctggggacag cgtcaacagc
      841 cttctcagca tcctggatgt catcaccggt caccagcatc cccagcggac cgtgtctgcc
      901 cgggcggccc gggactccac ggtgctgcgc ctgccggtgg aagcattctc cgcggtcttc
      961 accaagtacc cggagagctt ggtgcgggtc gtgcagatca tcatggtgcg gctgcagcga
     1021 gtcaccttcc tggcactgca caactacctg ggtctgacca atgagctctt cagccacgag
     1081 atccagcccc tgcgtctgtt ccccagcccc ggcctcccaa ctcgcaccag ccctgtgcgg
     1141 ggctccaaga gaatggtcag cacctcagct acagacgagc ccagggagac cccagggcgg
     1201 ccacccgatc ccaccggggc cccgctgcct ggacctacag gggaccctgt gaagcccaca
     1261 tccctggaaa ccccctcggc ccctctgctg agccgctgcg tctccatgcc aggggacatc
     1321 tcaggcttgc agggtggccc ccgctccgac ttcgacatgg cctatgagcg tggccggatc
     1381 tccgtgtccc tgcaggaaga ggcctccggg gggtccctgg cagcccccgc tcggaccccc
     1441 actcaggagc ctcgtgagca gccggcaggc gcctgtgaat acagctactg tgaggatgag
     1501 tcggccactg gtggctgccc tttcgggccc taccagggcc gccagaccag cagcatcttc
     1561 gaggcagcaa agcaggagct ggccaagctg atgcggattg aggacccctc cctcctgaac
     1621 agcagagtct tgctgcacca cgccaaagct ggcaccatca ttgcccgcca gggagaccag
     1681 gacgtgagcc tgcacttcgt gctctggggc tgcctgcacg tgtaccagcg catgatcgac
     1741 aaggcggagg acgtgtgcct gttcgtagcg cagcccgggg aactggtggg gcagctggcg
     1801 gtgctcactg gcgaacctct catcttcaca ctgcgagccc aacgcgactg caccttcctg
     1861 cggatctcca agtccgactt ctatgagatc atgcgcgcac agcccagtgt ggtgctgagt
     1921 gcggcgcaca cggtggcagc caggatgtcg cccttcgtgc gccagatgga cttcgccatc
     1981 gactggactg cagtggaggc gggacgcgcg ctgtacaggc agggcgaccg ctccgactgc
     2041 acttacatcg tgctcaatgg gcggctgcgt agcgtgatcc agcgaggcag tggcaagaag
     2101 gagctggtgg gcgagtacgg ccgcggcgac ctcatcggcg tggtggaggc actgacccgg
     2161 cagccgcgag ccacgacggt gcacgcggtg cgcgacacgg agctggccaa gcttcccgag
     2221 ggcaccttgg gtcacatcaa acgccggtac ccgcaggtcg tgacccgcct tatccaccta
     2281 ctgagccaga aaattctagg gaatttgcag cagctgcaag gacccttccc aggctctggg
     2341 ttgggtgtgc ccccacactc ggaactcacc aacccagcca gcaacctggc aactgtggca
     2401 atcctgcctg tgtgtgctga ggtccccatg gtggccttca cgctggagct gcagcacgcc
     2461 ctgcaggcca tcggtccgac gctactcctt aacagtgaca tcatccgggc acgcctgggg
     2521 gcctccgcac tggatagcat ccaagagttc cggctgtcag ggtggctggc ccagcaggag
     2581 gatgcacacc gtatcgtact ctaccagacg gacgcctcgc tgacgccctg gaccgtgcgc
     2641 tgcctgcgac aggccgactg catcctcatt gtgggcctgg gggaccagga gcctaccctc
     2701 ggccagctgg agcagatgct ggagaacacg gctgtgcgcg cccttaagca gctagtcctg
     2761 ctccaccgag aggagggcgc gggccccacg cgcaccgtgg agtggctaaa tatgcgcagc
     2821 tggtgctcgg ggcacctgca cctgcgctgt ccgcgccgcc tcttttcgcg ccgcagccct
     2881 gccaagctgc atgagctcta cgagaaggtt ttctccaggc gcgcggaccg gcacagcgac
     2941 ttctcccgct tggcgagggt gctcacgggg aacaccattg cccttgtgct aggcgggggc
     3001 ggggccaggg gctgctcgca catcggagta ctaaaggcat tagaggaggc gggggtcccc
     3061 gtggacctgg tgggcggcac gtccattggc tctttcatcg gagcgttgta cgcggaggag
     3121 cgcagcgcca gccgcacgaa gcagcgggcc cgggagtggg ccaagagcat gacttcggtg
     3181 ctggaacctg tgttggacct cacgtaccca gtcacctcca tgttcactgg gtctgccttt
     3241 aaccgcagca tccatcgggt cttccaggat aagcagattg aggacctgtg gctgccttac
     3301 ttcaacgtga ccacagatat caccgcctca gccatgcgag tccacaaaga tggctccctg
     3361 tggcggtacg tgcgcgccag catgacgctg tcgggctacc tgcccccgct gtgcgacccc
     3421 aaggacgggc acctactcat ggatggcggc tacatcaaca atctgccagc ggacatcgcc
     3481 cgcagcatgg gtgccaaaac ggtcatcgcc attgacgtgg ggagccagga tgagacggac
     3541 ctcagcacct acggggacag cctgtccggc tggtggctgc tgtggaagcg gctgaatccc
     3601 tgggctgaca aggtaaaggt tccagacatg gctgaaatcc agtcccgcct ggcctacgtg
     3661 tcctgtgtgc ggcagctaga ggttgtcaag tccagctcct actgcgagta cctgcgcccg
     3721 cccatcgact gcttcaagac catggacttt gggaagttcg accagatcta tgatgtgggc
     3781 taccagtacg ggaaggcggt gtttggaggc tggagccgtg gcaacgtcat tgagaaaatg
     3841 ctcacagacc ggcggtctac agaccttaat gagagccgcc gtgcagacgt gcttgccttc
     3901 ccaagctctg gcttcactga cttggcagag attgtgtccc ggattgagcc ccccacgagc
     3961 tatgtctctg atggctgtgc tgacggagag gagtcagatt gtctgacaga gtatgaggag
     4021 gacgccggac ccgactgctc gagggatgaa ggggggtccc ccgagggcgc aagccccagc
     4081 actgcctccg agatggagga ggagaagtcg attctccggc aacgacgctg tctgccccag
     4141 gagccgcccg gctcagccac agatgcctga ggacctcgac aggggtcacc ccctccctcc
     4201 cacccctgga ctgggctggg ggtggccccg tgggggtagc tcactccccc tcctgctgct
     4261 atgcctgtga cccccgcggc ccacacactg gactgacctg ccctgagcgg ggatgcagtg
     4321 ttgcactgat gacttgacca gcccctcccc caataaactc gcctcttgga aa
//
