LOCUS       NM_006228               1060 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens prepronociceptin (PNOC), transcript variant 1, mRNA.
ACCESSION   NM_006228
VERSION     NM_006228.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Chen,Z., Yu,M., Yan,J., Guo,L., Zhang,B., Liu,S., Lei,J., Zhang,W.,
            Zhou,B., Gao,J., Yang,Z., Li,X., Zhou,J., Fan,J., Ye,Q., Li,H.,
            Xu,Y. and Xiao,Y.
  TITLE     PNOC Expressed by B Cells in Cholangiocarcinoma Was Survival
            Related and LAIR2 Could Be a T Cell Exhaustion Biomarker in Tumor
            Microenvironment: Characterization of Immune Microenvironment
            Combining Single-Cell and Bulk Sequencing Technology
  JOURNAL   Front Immunol 12, 647209 (2021)
   PUBMED   33841428
  REMARK    GeneRIF: PNOC Expressed by B Cells in Cholangiocarcinoma Was
            Survival Related and LAIR2 Could Be a T Cell Exhaustion Biomarker
            in Tumor Microenvironment: Characterization of Immune
            Microenvironment Combining Single-Cell and Bulk Sequencing
            Technology.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Wachman,E.M., Hayes,M.J., Sherva,R., Brown,M.S., Shrestha,H.,
            Logan,B.A., Heller,N.A., Nielsen,D.A. and Farrer,L.A.
  TITLE     Association of maternal and infant variants in PNOC and COMT genes
            with neonatal abstinence syndrome severity
  JOURNAL   Am J Addict 26 (1), 42-49 (2017)
   PUBMED   27983768
  REMARK    GeneRIF: found differences in Neonatal Abstinence Syndrome outcomes
            depending on PNOC and COMT SNP genotype.
REFERENCE   3  (bases 1 to 1060)
  AUTHORS   Kraft,W.K., Stover,M.W. and Davis,J.M.
  TITLE     Neonatal abstinence syndrome: Pharmacologic strategies for the
            mother and infant
  JOURNAL   Semin Perinatol 40 (3), 203-212 (2016)
   PUBMED   26791055
  REMARK    GeneRIF: The presence of the PNOC rs732636 A allele was associated
            with the worse Neonatal abstinence syndrome outcomes in infants.
            Review article
REFERENCE   4  (bases 1 to 1060)
  AUTHORS   Lutz,P.E., Zhou,Y., Labbe,A., Mechawar,N. and Turecki,G.
  TITLE     Decreased expression of nociceptin/orphanin FQ in the dorsal
            anterior cingulate cortex of suicides
  JOURNAL   Eur Neuropsychopharmacol 25 (11), 2008-2014 (2015)
   PUBMED   26349406
  REMARK    GeneRIF: Study presents evidence suggesting that the PNOC and OPRL1
            are dysregulated in suicide completers, thereby potentially
            contributing to impaired emotional and behavioral control and,
            ultimately, to the suicidal crisis
REFERENCE   5  (bases 1 to 1060)
  AUTHORS   Wachman,E.M., Hayes,M.J., Sherva,R., Brown,M.S., Davis,J.M.,
            Farrer,L.A. and Nielsen,D.A.
  TITLE     Variations in opioid receptor genes in neonatal abstinence syndrome
  JOURNAL   Drug Alcohol Depend 155, 253-259 (2015)
   PUBMED   26233486
  REMARK    GeneRIF: findings suggest that SNPs in opioid receptors and the
            PNOC genes are associated with NAS severity.
REFERENCE   6  (bases 1 to 1060)
  AUTHORS   Witkin,J.M., Statnick,M.A., Rorick-Kehn,L.M., Pintar,J.E.,
            Ansonoff,M., Chen,Y., Tucker,R.C. and Ciccocioppo,R.
  TITLE     The biology of Nociceptin/Orphanin FQ (N/OFQ) related to obesity,
            stress, anxiety, mood, and drug dependence
  JOURNAL   Pharmacol Ther 141 (3), 283-299 (2014)
   PUBMED   24189487
  REMARK    Review article
            Erratum:[Pharmacol Ther. 2014 Sep;143(3):351]
REFERENCE   7  (bases 1 to 1060)
  AUTHORS   Nothacker,H.P., Reinscheid,R.K., Mansour,A., Henningsen,R.A.,
            Ardati,A., Monsma,F.J. Jr., Watson,S.J. and Civelli,O.
  TITLE     Primary structure and tissue distribution of the orphanin FQ
            precursor
  JOURNAL   Proc Natl Acad Sci U S A 93 (16), 8677-8682 (1996)
   PUBMED   8710930
REFERENCE   8  (bases 1 to 1060)
  AUTHORS   Mollereau,C., Simons,M.J., Soularue,P., Liners,F., Vassart,G.,
            Meunier,J.C. and Parmentier,M.
  TITLE     Structure, tissue distribution, and chromosomal localization of the
            prepronociceptin gene
  JOURNAL   Proc Natl Acad Sci U S A 93 (16), 8666-8670 (1996)
   PUBMED   8710928
REFERENCE   9  (bases 1 to 1060)
  AUTHORS   Reinscheid,R.K., Ardati,A., Monsma,F.J. Jr. and Civelli,O.
  TITLE     Structure-activity relationship studies on the novel neuropeptide
            orphanin FQ
  JOURNAL   J Biol Chem 271 (24), 14163-14168 (1996)
   PUBMED   8662940
REFERENCE   10 (bases 1 to 1060)
  AUTHORS   Connor,M., Yeo,A. and Henderson,G.
  TITLE     The effect of nociceptin on Ca2+ channel current and intracellular
            Ca2+ in the SH-SY5Y human neuroblastoma cell line
  JOURNAL   Br J Pharmacol 118 (2), 205-207 (1996)
   PUBMED   8735615
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC021678.13 and BC034758.1.
            
            On Nov 22, 2018 this sequence version replaced NM_006228.4.
            
            Summary: This gene encodes a preproprotein that is proteolytically
            processed to generate multiple protein products. These products
            include nociceptin, nocistatin, and orphanin FQ2 (OFQ2).
            Nociceptin, also known as orphanin FQ, is a 17-amino acid
            neuropeptide that binds to the nociceptin receptor to induce
            increased pain sensitivity, and may additionally regulate body
            temperature, learning and memory, and hunger. Another product of
            the encoded preproprotein, nocistatin, may inhibit the effects of
            nociceptin. [provided by RefSeq, Jul 2015].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC034758.1, U48263.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000301908.8/ ENSP00000301908.3
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                AC021678.13        152064-152082
            20-1060             BC034758.1         1-1041
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p21.1"
     gene            1..1060
                     /gene="PNOC"
                     /gene_synonym="N/OFQ; NOP; OFQ; ppN/OFQ; PPNOC"
                     /note="prepronociceptin"
                     /db_xref="GeneID:5368"
                     /db_xref="HGNC:HGNC:9163"
                     /db_xref="MIM:601459"
     exon            1..49
                     /gene="PNOC"
                     /gene_synonym="N/OFQ; NOP; OFQ; ppN/OFQ; PPNOC"
                     /inference="alignment:Splign:2.1.0"
     exon            50..198
                     /gene="PNOC"
                     /gene_synonym="N/OFQ; NOP; OFQ; ppN/OFQ; PPNOC"
                     /inference="alignment:Splign:2.1.0"
     CDS             73..603
                     /gene="PNOC"
                     /gene_synonym="N/OFQ; NOP; OFQ; ppN/OFQ; PPNOC"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; nocistatin; nociceptin; pronociceptin; orphanin
                     FQ; pre-pro-N/OFQ"
                     /codon_start=1
                     /product="prepronociceptin isoform 1 preproprotein"
                     /protein_id="NP_006219.1"
                     /db_xref="CCDS:CCDS6066.1"
                     /db_xref="GeneID:5368"
                     /db_xref="HGNC:HGNC:9163"
                     /db_xref="MIM:601459"
                     /translation="MKVLLCDLLLLSLFSSVFSSCQRDCLTCQEKLHPALDSFDLEVC
                     ILECEEKVFPSPLWTPCTKVMARSSWQLSPAAPEHVAAALYQPRASEMQHLRRMPRVR
                     SLFQEQEEPEPGMEEAGEMEQKQLQKRFGGFTGARKSARKLANQKRFSEFMRQYLVLS
                     MQSSQRRRTLHQNGNV"
     exon            199..650
                     /gene="PNOC"
                     /gene_synonym="N/OFQ; NOP; OFQ; ppN/OFQ; PPNOC"
                     /inference="alignment:Splign:2.1.0"
     exon            651..1060
                     /gene="PNOC"
                     /gene_synonym="N/OFQ; NOP; OFQ; ppN/OFQ; PPNOC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1033..1038
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PNOC"
                     /gene_synonym="N/OFQ; NOP; OFQ; ppN/OFQ; PPNOC"
                     /note="hexamer: ATTAAA"
     polyA_site      1060
                     /gene="PNOC"
                     /gene_synonym="N/OFQ; NOP; OFQ; ppN/OFQ; PPNOC"
                     /note="major polyA site"
ORIGIN      
        1 aaccgagcct cgcatccatc ggagggagcc ggggactgac agctctcagc acctgcttcc
       61 tgctcctgca ccatgaaagt cctgctttgt gacctgctgc tgctcagtct cttctccagt
      121 gtgttcagca gttgtcagag ggactgtctc acatgccagg agaagctcca cccagccctg
      181 gacagcttcg acctggaggt gtgcatcctc gagtgtgaag agaaggtctt ccccagcccc
      241 ctctggactc catgcaccaa ggtcatggcc aggagctctt ggcagctcag ccctgccgcc
      301 ccagagcatg tggcggctgc tctctaccag ccgagagctt cggagatgca gcatctgcgg
      361 cgaatgcccc gagtccggag cttgttccag gagcaggaag agcccgagcc tggcatggag
      421 gaggctggtg agatggagca gaagcagctg cagaagagat ttgggggctt caccggggcc
      481 cggaagtcgg ccaggaagtt ggccaatcag aagcggttca gtgagtttat gaggcaatac
      541 ttggtcctga gcatgcagtc cagccagcgc cggcgcaccc tgcaccagaa tggtaatgtg
      601 tagccggaag gggcgctcct cccagctgta ccggccactg caacccatga gcgtccaggt
      661 gatcccccaa acagcatgtg ctcagcccca gacctgccgc ctgggaatca ggattccttc
      721 ttccccaagg cactgagcgc ctgcagatcc cgcaggcttc gtttgcctcc agaaccttcc
      781 cgtctgattg ttcctcccca gccccctggc atgtttcacc acaaccctgt tgctacatca
      841 gagtgtattt ttgtaattcc tctagctacc atttcaatag ccccatctct cctgctcacc
      901 cgcctcttgc cccttctagg ggcaggtgaa aggaatagga aattgaacct ggggttttga
      961 cttgccactg ccataacttg tttgtaaaag agctgttctt tttgactgat tgttttaaac
     1021 aacgatttct ccattaaact tctactgagc aaatggttaa
//
