LOCUS       NM_000535               5093 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens PMS1 homolog 2, mismatch repair system component
            (PMS2), transcript variant 1, mRNA.
ACCESSION   NM_000535 XM_208368
VERSION     NM_000535.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Matoy,E.J., Plowman,J.N., Watson,C.J., Belshan,M.A., Blue,E.E.,
            Huff,C.D. and Stessman,H.A.F.
  TITLE     In vitro data suggest a role for PMS2 Kozak sequence mutations in
            Lynch syndrome risk
  JOURNAL   HGG Adv 5 (3), 100298 (2024)
   PUBMED   38654521
  REMARK    GeneRIF: In vitro data suggest a role for PMS2 Kozak sequence
            mutations in Lynch syndrome risk.
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Marin,F., Canet-Hermida,J., Bianchi,V., Chung,J., Wimmer,K.,
            Foulkes,W., Perez-Alonso,V., Dominguez-Pinilla,N., Sabado,C.,
            Vazquez-Gomez,F., Molines,A., Fioravantti,V., Carrasco,E.,
            Stengs,L., Edwards,M., Negm,L., Das,A., Aronson,M., Pastor,A.,
            Rueda,D., Gonzalez-Granado,L.I., Tabori,U., Capella,G. and
            Pineda,M.
  TITLE     A Validated Highly Sensitive Microsatellite Instability Assay
            Accurately Identifies Individuals Harboring Biallelic Germline PMS2
            Pathogenic Variants in Constitutional Mismatch Repair Deficiency
  JOURNAL   Clin Chem 70 (5), 737-746 (2024)
   PUBMED   38531023
  REMARK    GeneRIF: A Validated Highly Sensitive Microsatellite Instability
            Assay Accurately Identifies Individuals Harboring Biallelic
            Germline PMS2 Pathogenic Variants in Constitutional Mismatch Repair
            Deficiency.
REFERENCE   3  (bases 1 to 5093)
  AUTHORS   Sacho,E.J., Kadyrov,F.A., Modrich,P., Kunkel,T.A. and Erie,D.A.
  TITLE     Direct visualization of asymmetric adenine-nucleotide-induced
            conformational changes in MutL alpha
  JOURNAL   Mol Cell 29 (1), 112-121 (2008)
   PUBMED   18206974
  REMARK    GeneRIF: Adenine nucleotides induce large asymmetric conformational
            changes in full-length human MutL alpha. These changes are
            associated with significant increases in secondary structure.
REFERENCE   4  (bases 1 to 5093)
  AUTHORS   Kadyrov,F.A., Dzantiev,L., Constantin,N. and Modrich,P.
  TITLE     Endonucleolytic function of MutLalpha in human mismatch repair
  JOURNAL   Cell 126 (2), 297-308 (2006)
   PUBMED   16873062
REFERENCE   5  (bases 1 to 5093)
  AUTHORS   Constantin,N., Dzantiev,L., Kadyrov,F.A. and Modrich,P.
  TITLE     Human mismatch repair: reconstitution of a nick-directed
            bidirectional reaction
  JOURNAL   J Biol Chem 280 (48), 39752-39761 (2005)
   PUBMED   16188885
REFERENCE   6  (bases 1 to 5093)
  AUTHORS   Nicolaides,N.C., Carter,K.C., Shell,B.K., Papadopoulos,N.,
            Vogelstein,B. and Kinzler,K.W.
  TITLE     Genomic organization of the human PMS2 gene family
  JOURNAL   Genomics 30 (2), 195-206 (1995)
   PUBMED   8586419
REFERENCE   7  (bases 1 to 5093)
  AUTHORS   Nicolaides,N.C., Kinzler,K.W. and Vogelstein,B.
  TITLE     Analysis of the 5' region of PMS2 reveals heterogeneous transcripts
            and a novel overlapping gene
  JOURNAL   Genomics 29 (2), 329-334 (1995)
   PUBMED   8666379
REFERENCE   8  (bases 1 to 5093)
  AUTHORS   Hamilton,S.R., Liu,B., Parsons,R.E., Papadopoulos,N., Jen,J.,
            Powell,S.M., Krush,A.J., Berk,T., Cohen,Z., Tetu,B. et al.
  TITLE     The molecular basis of Turcot's syndrome
  JOURNAL   N Engl J Med 332 (13), 839-847 (1995)
   PUBMED   7661930
REFERENCE   9  (bases 1 to 5093)
  AUTHORS   Nicolaides,N.C., Papadopoulos,N., Liu,B., Wei,Y.F., Carter,K.C.,
            Ruben,S.M., Rosen,C.A., Haseltine,W.A., Fleischmann,R.D.,
            Fraser,C.M. et al.
  TITLE     Mutations of two PMS homologues in hereditary nonpolyposis colon
            cancer
  JOURNAL   Nature 371 (6492), 75-80 (1994)
   PUBMED   8072530
REFERENCE   10 (bases 1 to 5093)
  AUTHORS   Idos,G. and Valle,L.
  TITLE     Lynch Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301390
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA774159.1, BC093921.1,
            BP251389.1, AK294661.1, AK312390.1, AI433648.1, DB548617.1,
            AC005995.3 and BX537558.1.
            
            On Nov 23, 2018 this sequence version replaced NM_000535.6.
            
            Summary: The protein encoded by this gene is a key component of the
            mismatch repair system that functions to correct DNA mismatches and
            small insertions and deletions that can occur during DNA
            replication and homologous recombination. This protein forms
            heterodimers with the gene product of the mutL homolog 1 (MLH1)
            gene to form the MutL-alpha heterodimer. The MutL-alpha heterodimer
            possesses an endonucleolytic activity that is activated following
            recognition of mismatches and insertion/deletion loops by the
            MutS-alpha and MutS-beta heterodimers, and is necessary for removal
            of the mismatched DNA. There is a DQHA(X)2E(X)4E motif found at the
            C-terminus of the protein encoded by this gene that forms part of
            the active site of the nuclease. Mutations in this gene have been
            associated with hereditary nonpolyposis colorectal cancer (HNPCC;
            also known as Lynch syndrome) and Turcot syndrome. [provided by
            RefSeq, Apr 2016].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.156178.1,
                                           SRR1803612.49903.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265849.12/ ENSP00000265849.7
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-181               DA774159.1         58-238
            182-809             BC093921.1         174-801
            810-1066            BP251389.1         337-593
            1067-1650           BC093921.1         1059-1642
            1651-1651           AK294661.1         1558-1558
            1652-1895           BC093921.1         1644-1887
            1896-2495           AK312390.1         1902-2501
            2496-2776           AI433648.1         1-281               c
            2777-3175           DB548617.1         58-456
            3176-4382           AC005995.3         70711-71917
            4383-5093           BX537558.1         1344-2054
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p22.1"
     gene            1..5093
                     /gene="PMS2"
                     /gene_synonym="HNPCC4; LYNCH4; MLH4; MMRCS4; PMS-2;
                     PMS2CL; PMSL2"
                     /note="PMS1 homolog 2, mismatch repair system component"
                     /db_xref="GeneID:5395"
                     /db_xref="HGNC:HGNC:9122"
                     /db_xref="MIM:600259"
     exon            1..53
                     /gene="PMS2"
                     /gene_synonym="HNPCC4; LYNCH4; MLH4; MMRCS4; PMS-2;
                     PMS2CL; PMSL2"
                     /inference="alignment:Splign:2.1.0"
     CDS             31..2619
                     /gene="PMS2"
                     /gene_synonym="HNPCC4; LYNCH4; MLH4; MMRCS4; PMS-2;
                     PMS2CL; PMSL2"
                     /note="isoform a is encoded by transcript variant 1;
                     mismatch repair endonuclease PMS2; PMS1 protein homolog 2;
                     DNA mismatch repair protein PMS2; PMS2 postmeiotic
                     segregation increased 2; PMS1 homolog 2, mismatch repair
                     protein"
                     /codon_start=1
                     /product="mismatch repair endonuclease PMS2 isoform a"
                     /protein_id="NP_000526.2"
                     /db_xref="CCDS:CCDS5343.1"
                     /db_xref="GeneID:5395"
                     /db_xref="HGNC:HGNC:9122"
                     /db_xref="MIM:600259"
                     /translation="MERAESSSTEPAKAIKPIDRKSVHQICSGQVVLSLSTAVKELVE
                     NSLDAGATNIDLKLKDYGVDLIEVSDNGCGVEEENFEGLTLKHHTSKIQEFADLTQVE
                     TFGFRGEALSSLCALSDVTISTCHASAKVGTRLMFDHNGKIIQKTPYPRPRGTTVSVQ
                     QLFSTLPVRHKEFQRNIKKEYAKMVQVLHAYCIISAGIRVSCTNQLGQGKRQPVVCTG
                     GSPSIKENIGSVFGQKQLQSLIPFVQLPPSDSVCEEYGLSCSDALHNLFYISGFISQC
                     THGVGRSSTDRQFFFINRRPCDPAKVCRLVNEVYHMYNRHQYPFVVLNISVDSECVDI
                     NVTPDKRQILLQEEKLLLAVLKTSLIGMFDSDVNKLNVSQQPLLDVEGNLIKMHAADL
                     EKPMVEKQDQSPSLRTGEEKKDVSISRLREAFSLRHTTENKPHSPKTPEPRRSPLGQK
                     RGMLSSSTSGAISDKGVLRPQKEAVSSSHGPSDPTDRAEVEKDSGHGSTSVDSEGFSI
                     PDTGSHCSSEYAASSPGDRGSQEHVDSQEKAPKTDDSFSDVDCHSNQEDTGCKFRVLP
                     QPTNLATPNTKRFKKEEILSSSDICQKLVNTQDMSASQVDVAVKINKKVVPLDFSMSS
                     LAKRIKQLHHEAQQSEGEQNYRKFRAKICPGENQAAEDELRKEISKTMFAEMEIIGQF
                     NLGFIITKLNEDIFIVDQHATDEKYNFEMLQQHTVLQGQRLIAPQTLNLTAVNEAVLI
                     ENLEIFRKNGFDFVIDENAPVTERAKLISLPTSKNWTFGPQDVDELIFMLSDSPGVMC
                     RPSRVKQMFASRACRKSVMIGTALNTSEMKKLITHMGEMDHPWNCPHGRPTMRHIANL
                     GVISQN"
     exon            54..193
                     /gene="PMS2"
                     /gene_synonym="HNPCC4; LYNCH4; MLH4; MMRCS4; PMS-2;
                     PMS2CL; PMSL2"
                     /inference="alignment:Splign:2.1.0"
     exon            194..280
                     /gene="PMS2"
                     /gene_synonym="HNPCC4; LYNCH4; MLH4; MMRCS4; PMS-2;
                     PMS2CL; PMSL2"
                     /inference="alignment:Splign:2.1.0"
     exon            281..383
                     /gene="PMS2"
                     /gene_synonym="HNPCC4; LYNCH4; MLH4; MMRCS4; PMS-2;
                     PMS2CL; PMSL2"
                     /inference="alignment:Splign:2.1.0"
     exon            384..567
                     /gene="PMS2"
                     /gene_synonym="HNPCC4; LYNCH4; MLH4; MMRCS4; PMS-2;
                     PMS2CL; PMSL2"
                     /inference="alignment:Splign:2.1.0"
     exon            568..735
                     /gene="PMS2"
                     /gene_synonym="HNPCC4; LYNCH4; MLH4; MMRCS4; PMS-2;
                     PMS2CL; PMSL2"
                     /inference="alignment:Splign:2.1.0"
     exon            736..833
                     /gene="PMS2"
                     /gene_synonym="HNPCC4; LYNCH4; MLH4; MMRCS4; PMS-2;
                     PMS2CL; PMSL2"
                     /inference="alignment:Splign:2.1.0"
     exon            834..933
                     /gene="PMS2"
                     /gene_synonym="HNPCC4; LYNCH4; MLH4; MMRCS4; PMS-2;
                     PMS2CL; PMSL2"
                     /inference="alignment:Splign:2.1.0"
     exon            934..1018
                     /gene="PMS2"
                     /gene_synonym="HNPCC4; LYNCH4; MLH4; MMRCS4; PMS-2;
                     PMS2CL; PMSL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1019..1174
                     /gene="PMS2"
                     /gene_synonym="HNPCC4; LYNCH4; MLH4; MMRCS4; PMS-2;
                     PMS2CL; PMSL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1175..2036
                     /gene="PMS2"
                     /gene_synonym="HNPCC4; LYNCH4; MLH4; MMRCS4; PMS-2;
                     PMS2CL; PMSL2"
                     /inference="alignment:Splign:2.1.0"
     exon            2037..2204
                     /gene="PMS2"
                     /gene_synonym="HNPCC4; LYNCH4; MLH4; MMRCS4; PMS-2;
                     PMS2CL; PMSL2"
                     /inference="alignment:Splign:2.1.0"
     exon            2205..2305
                     /gene="PMS2"
                     /gene_synonym="HNPCC4; LYNCH4; MLH4; MMRCS4; PMS-2;
                     PMS2CL; PMSL2"
                     /inference="alignment:Splign:2.1.0"
     exon            2306..2475
                     /gene="PMS2"
                     /gene_synonym="HNPCC4; LYNCH4; MLH4; MMRCS4; PMS-2;
                     PMS2CL; PMSL2"
                     /inference="alignment:Splign:2.1.0"
     exon            2476..5093
                     /gene="PMS2"
                     /gene_synonym="HNPCC4; LYNCH4; MLH4; MMRCS4; PMS-2;
                     PMS2CL; PMSL2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2728..2733
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PMS2"
                     /gene_synonym="HNPCC4; LYNCH4; MLH4; MMRCS4; PMS-2;
                     PMS2CL; PMSL2"
                     /note="hexamer: AATACA"
     polyA_site      2751
                     /gene="PMS2"
                     /gene_synonym="HNPCC4; LYNCH4; MLH4; MMRCS4; PMS-2;
                     PMS2CL; PMSL2"
                     /note="major polyA site"
     polyA_site      2777
                     /gene="PMS2"
                     /gene_synonym="HNPCC4; LYNCH4; MLH4; MMRCS4; PMS-2;
                     PMS2CL; PMSL2"
                     /note="major polyA site"
     regulatory      4846..4851
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PMS2"
                     /gene_synonym="HNPCC4; LYNCH4; MLH4; MMRCS4; PMS-2;
                     PMS2CL; PMSL2"
                     /note="hexamer: AATAAA"
     polyA_site      4868
                     /gene="PMS2"
                     /gene_synonym="HNPCC4; LYNCH4; MLH4; MMRCS4; PMS-2;
                     PMS2CL; PMSL2"
     regulatory      5064..5069
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PMS2"
                     /gene_synonym="HNPCC4; LYNCH4; MLH4; MMRCS4; PMS-2;
                     PMS2CL; PMSL2"
                     /note="hexamer: AATAAA"
     polyA_site      5093
                     /gene="PMS2"
                     /gene_synonym="HNPCC4; LYNCH4; MLH4; MMRCS4; PMS-2;
                     PMS2CL; PMSL2"
                     /note="major polyA site"
ORIGIN      
        1 agtccccgag gcggatcggg tgttgcatcc atggagcgag ctgagagctc gagtacagaa
       61 cctgctaagg ccatcaaacc tattgatcgg aagtcagtcc atcagatttg ctctgggcag
      121 gtggtactga gtctaagcac tgcggtaaag gagttagtag aaaacagtct ggatgctggt
      181 gccactaata ttgatctaaa gcttaaggac tatggagtgg atcttattga agtttcagac
      241 aatggatgtg gggtagaaga agaaaacttc gaaggcttaa ctctgaaaca tcacacatct
      301 aagattcaag agtttgccga cctaactcag gttgaaactt ttggctttcg gggggaagct
      361 ctgagctcac tttgtgcact gagcgatgtc accatttcta cctgccacgc atcggcgaag
      421 gttggaactc gactgatgtt tgatcacaat gggaaaatta tccagaaaac cccctacccc
      481 cgccccagag ggaccacagt cagcgtgcag cagttatttt ccacactacc tgtgcgccat
      541 aaggaatttc aaaggaatat taagaaggag tatgccaaaa tggtccaggt cttacatgca
      601 tactgtatca tttcagcagg catccgtgta agttgcacca atcagcttgg acaaggaaaa
      661 cgacagcctg tggtatgcac aggtggaagc cccagcataa aggaaaatat cggctctgtg
      721 tttgggcaga agcagttgca aagcctcatt ccttttgttc agctgccccc tagtgactcc
      781 gtgtgtgaag agtacggttt gagctgttcc gatgctctgc ataatctttt ttacatctca
      841 ggtttcattt cacaatgcac gcatggagtt ggaaggagtt caacagacag acagtttttc
      901 tttatcaacc ggcggccttg tgacccagca aaggtctgca gactcgtgaa tgaggtctac
      961 cacatgtata atcgacacca gtatccattt gttgttctta acatttctgt tgattcagaa
     1021 tgcgttgata tcaatgttac tccagataaa aggcaaattt tgctacaaga ggaaaagctt
     1081 ttgttggcag ttttaaagac ctctttgata ggaatgtttg atagtgatgt caacaagcta
     1141 aatgtcagtc agcagccact gctggatgtt gaaggtaact taataaaaat gcatgcagcg
     1201 gatttggaaa agcccatggt agaaaagcag gatcaatccc cttcattaag gactggagaa
     1261 gaaaaaaaag acgtgtccat ttccagactg cgagaggcct tttctcttcg tcacacaaca
     1321 gagaacaagc ctcacagccc aaagactcca gaaccaagaa ggagccctct aggacagaaa
     1381 aggggtatgc tgtcttctag cacttcaggt gccatctctg acaaaggcgt cctgagacct
     1441 cagaaagagg cagtgagttc cagtcacgga cccagtgacc ctacggacag agcggaggtg
     1501 gagaaggact cggggcacgg cagcacttcc gtggattctg aggggttcag catcccagac
     1561 acgggcagtc actgcagcag cgagtatgcg gccagctccc caggggacag gggctcgcag
     1621 gaacatgtgg actctcagga gaaagcgcct aaaactgacg actctttttc agatgtggac
     1681 tgccattcaa accaggaaga taccggatgt aaatttcgag ttttgcctca gccaactaat
     1741 ctcgcaaccc caaacacaaa gcgttttaaa aaagaagaaa ttctttccag ttctgacatt
     1801 tgtcaaaagt tagtaaatac tcaggacatg tcagcctctc aggttgatgt agctgtgaaa
     1861 attaataaga aagttgtgcc cctggacttt tctatgagtt ctttagctaa acgaataaag
     1921 cagttacatc atgaagcaca gcaaagtgaa ggggaacaga attacaggaa gtttagggca
     1981 aagatttgtc ctggagaaaa tcaagcagcc gaagatgaac taagaaaaga gataagtaaa
     2041 acgatgtttg cagaaatgga aatcattggt cagtttaacc tgggatttat aataaccaaa
     2101 ctgaatgagg atatcttcat agtggaccag catgccacgg acgagaagta taacttcgag
     2161 atgctgcagc agcacaccgt gctccagggg cagaggctca tagcacctca gactctcaac
     2221 ttaactgctg ttaatgaagc tgttctgata gaaaatctgg aaatatttag aaagaatggc
     2281 tttgattttg ttatcgatga aaatgctcca gtcactgaaa gggctaaact gatttccttg
     2341 ccaactagta aaaactggac cttcggaccc caggacgtcg atgaactgat cttcatgctg
     2401 agcgacagcc ctggggtcat gtgccggcct tcccgagtca agcagatgtt tgcctccaga
     2461 gcctgccgga agtcggtgat gattgggact gctcttaaca caagcgagat gaagaaactg
     2521 atcacccaca tgggggagat ggaccacccc tggaactgtc cccatggaag gccaaccatg
     2581 agacacatcg ccaacctggg tgtcatttct cagaactgac cgtagtcact gtatggaata
     2641 attggtttta tcgcagattt ttatgttttg aaagacagag tcttcactaa ccttttttgt
     2701 tttaaaatga acctgctact taaaaaaaat acacatcaca cccatttaaa agtgatcttg
     2761 agaacctttt caaaccagat ggagcattgc ttgcaaattt tttttctcta tgtttgcatg
     2821 cgctcgtgtg tgtgtgtcca ggcaagaaca cattttataa aaataagaac acttgggctg
     2881 ggcatggtgg ctcatgcctg tgatcgcagc actttgggag gccgaggccg gcggatcacc
     2941 tgagatcaga agttcgagac cagcctgacc aacatggaga aaccctgcct ctactaaaaa
     3001 tacaaaatta gccaggtgtg ctggcgcatg cctgtaatcc ccgctaccca ggaggctgag
     3061 gcaggagaat cgcttgaacc cgggagacgg aggttgcagt gaaccgagat tgcgccactg
     3121 cgctccagcc tgggtgagat agaacaagac tgtgtctcaa aaaacaaaac aaaacaaaac
     3181 aaaaaaaaaa aaaccaaacc actttggaag ttactcaggc ctctgctctg gctggacata
     3241 gtttagtcta taactttcaa cccttaatga taattaaatt catctttgtt taatttcata
     3301 aatttaaaag tagggtcctt ttcagttagt gattctcagc cctgattcac attaaatttt
     3361 taaacacggg ggattctctg cccggctgga agaaaatgac tggatgggac aggggtcact
     3421 atttgaaaca ttcctctgtg cggccaaggt cgcaaaatgc tgtcctcgca ggggaacaaa
     3481 aagagtttga tttcccataa tttgatgctg tgatttggtt tcctcaggat gtgaactgta
     3541 gaacattcca gttactggcc ttgaatggtt ctgggaatat aagaatccct gtctgtcttt
     3601 tcaaatagtt ttcatggaac cttgtcctgt ttgaacttgg ctgaaaatgg aagtaaagat
     3661 gccctcttgg gggcccagag atgacagatg tggctccccc tgctgccccc accccttctc
     3721 cagactgtgg gcggctcccc ttcctgcttt agaatccctc agatggagga ggcagtacag
     3781 tagtcactgt gccatcgtgt ctggcactgt gctggcgtgg tctgcaggat cccacttatg
     3841 aactctccag attgggagct gtggcaggat aacagccccc aagacagctg tgtcctaatc
     3901 cccagaacct gtgaccacgc tgcctcacgt ggcagaaggg actcggcagg tgtgattgag
     3961 tgaaggatct tttttttttt tttctttgag atgaagtttc gctcttgttg cccaggctgg
     4021 agttcaatag catgatctca gctcactgca gcctctgcct cccaggttca agtgattctc
     4081 ccacctcagc ctcccgagta gctgggatta caggtgtcca gaaccatact ggctaatttt
     4141 tgtattttta gtagagacag ggtttcacca tgttgaccag gctggtctcg aactcctgac
     4201 ctcaggtgat ccgaccgcct cggcctccca aagtgctggg attacaggtg tgagccatca
     4261 tgcctggctg agttaaggat cttgcaacag agagattatc ctggattgtc tgggtgggcc
     4321 cagtccattg ggtgagtcct tcaaaggtgg agacctttcc ctgctggcca gagagaggct
     4381 gtcttgctgg ttttggagat ggaaggaggt accactagtc aaggattgca agcagtctct
     4441 agaacaggga ttccaacact ccggacacag accagtagtg gtccatggcc tattaggaag
     4501 tggggtgcac agcaggttag gggccggcaa gccagcgaag cttcatctgt atttatagcc
     4561 actccccgtc gctggcgtta ccacccgagc tccgcctcct gtcacatcag cggtgggcat
     4621 tagattctca tagcagcacg agccctattg tgaactgcac acacgaggga tgtaggttgc
     4681 acgctcctta tgagaatctg atgcctgatg atctgtcact gtctcccgtc acccccagat
     4741 ggggctgtct agttgcagga aaacaagctc agggctccca ctgagtctct gtgatggtga
     4801 gttgtagaat tatttaatta tatgttacaa tgtaataata gtagaaataa agtgcacaat
     4861 aaatgcaatg cacttgaatc gtcctgaaac catccctccc cgaccccaat ccatggaaaa
     4921 attgtgttcc gcgaaaccgg tctctggtgc caaaaaggtt ggggaccgct tctggaaaag
     4981 ctggaaaagg caagaaaacg cattctctcc ctcagcctct ggaaggaacc agcactgtgg
     5041 gactaattta catactgtag ggtaataaat ttgtgttgct tcgaaccact aaa
//
