LOCUS       NM_020353               3233 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens phospholipid scramblase 4 (PLSCR4), transcript variant
            2, mRNA.
ACCESSION   NM_020353
VERSION     NM_020353.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Barth,L.A.G., Nebe,M., Kalwa,H., Velluva,A., Kehr,S., Kolbig,F.,
            Prabutzki,P., Kiess,W., Le Duc,D., Garten,A. and Kirstein,A.S.
  TITLE     Phospholipid Scramblase 4 (PLSCR4) Regulates Adipocyte
            Differentiation via PIP3-Mediated AKT Activation
  JOURNAL   Int J Mol Sci 23 (17), 9787 (2022)
   PUBMED   36077184
  REMARK    GeneRIF: Phospholipid Scramblase 4 (PLSCR4) Regulates Adipocyte
            Differentiation via PIP3-Mediated AKT Activation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Abdulwahab,H., Aljishi,M., Sultan,A., Al-Kafaji,G., Sridharan,K.,
            Bakhiet,M. and Taha,S.
  TITLE     Whole blood transcriptomic analysis reveals PLSCR4 as a potential
            marker for vaso-occlusive crises in sickle cell disease
  JOURNAL   Sci Rep 11 (1), 22199 (2021)
   PUBMED   34772994
  REMARK    GeneRIF: Whole blood transcriptomic analysis reveals PLSCR4 as a
            potential marker for vaso-occlusive crises in sickle cell disease.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3233)
  AUTHORS   Xue,D., Xue,Y.F., Zhang,L.J., Cui,L.Z., Guo,K.Q. and Lian,J.
  TITLE     LINC00641 induces the malignant progression of colorectal carcinoma
            through the miRNA-424-5p/PLSCR4 feedback loop
  JOURNAL   Eur Rev Med Pharmacol Sci 25 (2), 749-757 (2021)
   PUBMED   33577029
  REMARK    GeneRIF: LINC00641 induces the malignant progression of colorectal
            carcinoma through the miRNA-424-5p/PLSCR4 feedback loop.
REFERENCE   4  (bases 1 to 3233)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 3233)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3233)
  AUTHORS   Lott,K., Bhardwaj,A., Sims,P.J. and Cingolani,G.
  TITLE     A minimal nuclear localization signal (NLS) in human phospholipid
            scramblase 4 that binds only the minor NLS-binding site of importin
            alpha1
  JOURNAL   J Biol Chem 286 (32), 28160-28169 (2011)
   PUBMED   21690087
  REMARK    GeneRIF: Crystallographic analysis of mammalian importin alpha1 in
            complex with the hPLSCR4-NLS reveals this minimal NLS binds
            specifically and exclusively to the minor binding site of importin
            alpha
REFERENCE   7  (bases 1 to 3233)
  AUTHORS   Yu,H., Tardivo,L., Tam,S., Weiner,E., Gebreab,F., Fan,C.,
            Svrzikapa,N., Hirozane-Kishikawa,T., Rietman,E., Yang,X.,
            Sahalie,J., Salehi-Ashtiani,K., Hao,T., Cusick,M.E., Hill,D.E.,
            Roth,F.P., Braun,P. and Vidal,M.
  TITLE     Next-generation sequencing to generate interactome datasets
  JOURNAL   Nat Methods 8 (6), 478-480 (2011)
   PUBMED   21516116
REFERENCE   8  (bases 1 to 3233)
  AUTHORS   Sahu,S.K., Aradhyam,G.K. and Gummadi,S.N.
  TITLE     Calcium binding studies of peptides of human phospholipid
            scramblases 1 to 4 suggest that scramblases are new class of
            calcium binding proteins in the cell
  JOURNAL   Biochim Biophys Acta 1790 (10), 1274-1281 (2009)
   PUBMED   19540310
  REMARK    GeneRIF: Results show that binding affinities of the peptides are
            in the order hPLSCR1>hPLSCR3>hPLSCR2>hPLSCR4 for Ca2+ and in the
            order hPLSCR1>hPLSCR2>hPLSCR3>hPLSCR4 for Mg2+.
REFERENCE   9  (bases 1 to 3233)
  AUTHORS   Py,B., Basmaciogullari,S., Bouchet,J., Zarka,M., Moura,I.C.,
            Benhamou,M., Monteiro,R.C., Hocini,H., Madrid,R. and Benichou,S.
  TITLE     The phospholipid scramblases 1 and 4 are cellular receptors for the
            secretory leukocyte protease inhibitor and interact with CD4 at the
            plasma membrane
  JOURNAL   PLoS One 4 (3), e5006 (2009)
   PUBMED   19333378
  REMARK    GeneRIF: SLPI is a ligand for PLSCR1 and PLSCR4, which also
            interact directly with the CD4 receptor at the cell surface of T
            lymphocytes
            Erratum:[PLoS ONE. 2009;4(4). doi:
            10.1371/annotation/657cd713-aaac-4ebb-80ad-3ec8dfb12b42]
REFERENCE   10 (bases 1 to 3233)
  AUTHORS   Wiedmer,T., Zhou,Q., Kwoh,D.Y. and Sims,P.J.
  TITLE     Identification of three new members of the phospholipid scramblase
            gene family
  JOURNAL   Biochim Biophys Acta 1467 (1), 244-253 (2000)
   PUBMED   10930526
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC028354.2, AF199023.1, AK128442.1, AC092982.3 and AW086426.1.
            
            On Nov 22, 2018 this sequence version replaced NM_020353.2.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1, 2, and 3 all encode isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF199023.1, SRR1660805.258551.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2467146 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000354952.7/ ENSP00000347038.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1621              BC028354.2         75-1695
            1622-2190           AF199023.1         1600-2168
            2191-2515           AK128442.1         1867-2191
            2516-2516           AC092982.3         65082-65082         c
            2517-3163           AK128442.1         2192-2838
            3164-3233           AW086426.1         6-75                c
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q24"
     gene            1..3233
                     /gene="PLSCR4"
                     /gene_synonym="TRA1"
                     /note="phospholipid scramblase 4"
                     /db_xref="GeneID:57088"
                     /db_xref="HGNC:HGNC:16497"
                     /db_xref="MIM:607612"
     exon            1..146
                     /gene="PLSCR4"
                     /gene_synonym="TRA1"
                     /inference="alignment:Splign:2.1.0"
     exon            147..174
                     /gene="PLSCR4"
                     /gene_synonym="TRA1"
                     /inference="alignment:Splign:2.1.0"
     CDS             168..1157
                     /gene="PLSCR4"
                     /gene_synonym="TRA1"
                     /note="isoform a is encoded by transcript variant 2; PL
                     scramblase 4; Ca(2+)-dependent phospholipid scramblase 4;
                     cell growth inhibiting protein 43"
                     /codon_start=1
                     /product="phospholipid scramblase 4 isoform a"
                     /protein_id="NP_065086.2"
                     /db_xref="CCDS:CCDS3133.1"
                     /db_xref="GeneID:57088"
                     /db_xref="HGNC:HGNC:16497"
                     /db_xref="MIM:607612"
                     /translation="MSGVVPTAPEQPAGEMENQTKPPDPRPDAPPEYNSHFLPGPPGT
                     AVPPPTGYPGGLPMGYYSPQQPSTFPLYQPVGGIHPVRYQPGKYPMPNQSVPITWMPG
                     PTPMANCPPGLEYLVQLDNIHVLQHFEPLEMMTCFETNNRYDIKNNSDQMVYIVTEDT
                     DDFTRNAYRTLRPFVLRVTDCMGREIMTMQRPFRCTCCCFCCPSARQELEVQCPPGVT
                     IGFVAEHWNLCRAVYSIQNEKKENVMRVRGPCSTYGCGSDSVFEVKSLDGISNIGSII
                     RKWNGLLSAMADADHFDIHFPLDLDVKMKAMIFGACFLIDFMYFERSPPQRSR"
     exon            175..285
                     /gene="PLSCR4"
                     /gene_synonym="TRA1"
                     /inference="alignment:Splign:2.1.0"
     exon            286..521
                     /gene="PLSCR4"
                     /gene_synonym="TRA1"
                     /inference="alignment:Splign:2.1.0"
     exon            522..564
                     /gene="PLSCR4"
                     /gene_synonym="TRA1"
                     /inference="alignment:Splign:2.1.0"
     exon            565..791
                     /gene="PLSCR4"
                     /gene_synonym="TRA1"
                     /inference="alignment:Splign:2.1.0"
     exon            792..953
                     /gene="PLSCR4"
                     /gene_synonym="TRA1"
                     /inference="alignment:Splign:2.1.0"
     exon            954..1112
                     /gene="PLSCR4"
                     /gene_synonym="TRA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1113..3233
                     /gene="PLSCR4"
                     /gene_synonym="TRA1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3143..3148
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLSCR4"
                     /gene_synonym="TRA1"
                     /note="hexamer: ATTAAA"
     polyA_site      3165
                     /gene="PLSCR4"
                     /gene_synonym="TRA1"
                     /note="major polyA site"
     regulatory      3210..3215
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLSCR4"
                     /gene_synonym="TRA1"
                     /note="hexamer: AATATA"
     polyA_site      3233
                     /gene="PLSCR4"
                     /gene_synonym="TRA1"
                     /note="major polyA site"
ORIGIN      
        1 ctcccttccg cgcgcttact ttgtttataa cttgaaaaat cctctccgtc tcccttccct
       61 gcctcctttc ctttcccttt cctctgccag tacaactaga cccggcgtct ggcgtccccg
      121 gtgcccagca ttctgcgggg caggcggatt aattggaatt cttcaaaatg tcaggtgtgg
      181 tacccacagc ccctgaacag cctgcaggtg aaatggaaaa tcaaacaaaa ccaccagatc
      241 caaggcctga tgctcctcct gaatacaatt ctcatttttt accaggaccc cctggaacag
      301 ctgtccctcc acctactggc tacccaggag gcttgcctat gggatactac agtccacagc
      361 aacccagtac cttccctttg taccagccag ttggtggtat ccatcctgtc cggtatcagc
      421 ctggcaaata tcctatgcca aatcagtctg ttccaataac atggatgcca gggccaactc
      481 ctatggcaaa ctgccctcct ggtctggaat acttagttca gttggacaac atacatgttc
      541 ttcagcattt tgagcctctg gaaatgatga catgttttga aactaataat agatatgata
      601 ttaaaaacaa ctcagaccag atggtttaca ttgtaaccga agacacagat gactttacca
      661 ggaatgccta tcggacacta aggcccttcg tcctccgggt cactgattgt atgggccgag
      721 aaatcatgac aatgcagaga cccttcagat gcacctgctg ttgcttctgt tgcccctctg
      781 ccagacaaga gctggaggtg cagtgtcctc ctggtgtcac cattggcttt gttgcggaac
      841 attggaacct gtgcagggcg gtgtacagca tccaaaatga gaagaaagaa aatgtgatga
      901 gagttcgtgg gccatgctca acctatggct gtggttcaga ttctgttttt gaggtcaaat
      961 cccttgatgg catatccaac atcggcagta ttatccggaa gtggaatggt ttgttatcag
     1021 caatggcaga tgctgaccat tttgacattc acttcccact agacctggat gtgaagatga
     1081 aagccatgat ttttggagct tgcttcctca ttgacttcat gtattttgaa agatctccac
     1141 cacaacgttc aagatagaga gacacagcaa gccatcaact atggttaatt ttgaaaaatg
     1201 gaaaagttgg attgggctta cagtcagcac tcagttattt gcaagtgtat ttctttgctt
     1261 tgtagagtat ttttattggg tgttaacttt gacagctgag agtgggcttg caagaacaca
     1321 atctaaaagt gtgtttcaat tgagtatctc tctagtagaa taggagttca tcctgaaaag
     1381 ctgtgactca ttaacccagt aaacatatac aaagtaagct taaaacacta taaacatgag
     1441 ataagggaaa atgaatccag agttctcata ttaataggta gtgaaacaat aaggcttttt
     1501 agagcagact ttgttggcat aaaataacct ggcttctatc cctaaccctt tcctaccttt
     1561 cctctccgtc aacatgtcct catactgaag acaaacttgt ttcaatgata gtcttcattt
     1621 ttaaaaacaa aaaggcaggc agacagaaat aatgatgttt tcttgcacta agaaggtact
     1681 acttgtacac atatatcaaa acctcattct gcaaagtttt tgaaggtttc aatgggaaat
     1741 ttgattttat tacaaaataa aacatttttt aatgttaaag tttatatatt ccatgcttgt
     1801 tttctcattc actggcatgg atgatcagga gctgcctata tatgaaggca gaatcagact
     1861 atcaggaaag gagctggcca gggccacagc cagtcaagat ctctgagcaa cttagagaca
     1921 ttggtgtcat tatatgaagc ttgcatttaa tacatttata cataatacat ttgtacattt
     1981 aattcataac gtctcttggt cacagatgcc ttatatataa aataagttgc cagatctcta
     2041 agattgccta gtacaccttt gtatctcatt tgatgtgata cccagaagag atcattgttt
     2101 tttgtttttg tttttgtttt tttcaagaag atccttcgtg atcaccatgc tgttctcatg
     2161 gtaagaactg gagttatgtt tttaaatttg gaaatatgac attttatgta gcactttata
     2221 aaaagtgaaa gcgacaaatt ccaccgctgc ttaatactgc tttgcttctt tttattgaca
     2281 tgatagataa atatgtatct acacagagta ataataataa aacacagtaa acattctatt
     2341 tctctatggt ctacagcatg ccagtaaata atatgtagga ccaataataa attatcaatt
     2401 acacattttt gtgttaacta attaaaagca tagtgtataa gtgagtacac tctaattaac
     2461 ttgcttctgt tgcactttag ttttctacct gcatatggac tgcatttttt ttttttaaca
     2521 cagtcagtat gtagaatggg atgtattctt ctgctgctgc ttattaaata aagaaagcct
     2581 gagtgttctt agatggggtt attctgagat gagggtctta gcctacagtt ctttttgaaa
     2641 tgaaaggtgc tttgtttttt aattatattc atcttttcag ggtaaatttg tttttctgag
     2701 tttctcgtaa tgctcatttt tacatgctgc tactagcttt tttttttaaa aaaagtaaaa
     2761 gttgctgctt tctaaaatat taattgcctt atatttgaaa gtgccattgc aatcgtaagt
     2821 agactatgta tttcctataa tgatgtctga tatttaaata ggaaatcaga caaacaatat
     2881 tcagaaagtt taagcatata aactttttat ttttaacttg cctaaatccc tgtattccaa
     2941 aacctgctgc atcataataa atatatctat atatatttag cataagacgt gatattttta
     3001 atttcttttt taaaaaatta tatttgtctc ttagagttaa aattttcttt atataatatt
     3061 gtcatatgtc atagttttaa tacaattcac atgatttcta tgtttcttaa tgatattttg
     3121 ttgtgtaaaa ttgatcggat tgattaaaaa acaaattctc tggaatttgt gcgttcatgc
     3181 tttttcgtat tctttatggc ttttaaataa atatacaatg gttaatagta aaa
//
