LOCUS       NM_020360               1752 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens phospholipid scramblase 3 (PLSCR3), transcript variant
            1, mRNA.
ACCESSION   NM_020360
VERSION     NM_020360.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1752)
  AUTHORS   Palanirajan,S.K. and Gummadi,S.N.
  TITLE     Heavy-Metals-Mediated Phospholipids Scrambling by Human
            Phospholipid Scramblase 3: A Probable Role in Mitochondrial
            Apoptosis
  JOURNAL   Chem Res Toxicol 33 (2), 553-564 (2020)
   PUBMED   31769662
REFERENCE   4  (bases 1 to 1752)
  AUTHORS   Palanirajan,S.K., Sivagnanam,U., Murugan,S. and Gummadi,S.N.
  TITLE     In vitro reconstitution and biochemical characterization of human
            phospholipid scramblase 3: phospholipid specificity and metal ion
            binding studies
  JOURNAL   Biol Chem 399 (4), 361-374 (2018)
   PUBMED   29337693
  REMARK    GeneRIF: The results revealed that Ca2+ and Mg2+ bind to Human
            phospholipid scramblase 3 (hPLSCR3) and trigger conformational
            changes mediated by aggregation.
REFERENCE   5  (bases 1 to 1752)
  AUTHORS   Zhong,Q., Pevzner,S.J., Hao,T., Wang,Y., Mosca,R., Menche,J.,
            Taipale,M., Tasan,M., Fan,C., Yang,X., Haley,P., Murray,R.R.,
            Mer,F., Gebreab,F., Tam,S., MacWilliams,A., Dricot,A., Reichert,P.,
            Santhanam,B., Ghamsari,L., Calderwood,M.A., Rolland,T.,
            Charloteaux,B., Lindquist,S., Barabasi,A.L., Hill,D.E., Aloy,P.,
            Cusick,M.E., Xia,Y., Roth,F.P. and Vidal,M.
  TITLE     An inter-species protein-protein interaction network across vast
            evolutionary distance
  JOURNAL   Mol Syst Biol 12 (4), 865 (2016)
   PUBMED   27107014
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1752)
  AUTHORS   He,Y., Liu,J., Grossman,D., Durrant,D., Sweatman,T., Lothstein,L.,
            Epand,R.F., Epand,R.M. and Lee,R.M.
  TITLE     Phosphorylation of mitochondrial phospholipid scramblase 3 by
            protein kinase C-delta induces its activation and facilitates
            mitochondrial targeting of tBid
  JOURNAL   J Cell Biochem 101 (5), 1210-1221 (2007)
   PUBMED   17226776
  REMARK    GeneRIF: These findings indicate that phosphorylation of PLS3 by
            PKC-delta induces PLS3 activation to facilitate mitochondrial
            targeting of tBid and apoptosis.
REFERENCE   7  (bases 1 to 1752)
  AUTHORS   Van,Q., Liu,J., Lu,B., Feingold,K.R., Shi,Y., Lee,R.M. and
            Hatch,G.M.
  TITLE     Phospholipid scramblase-3 regulates cardiolipin de novo
            biosynthesis and its resynthesis in growing HeLa cells
  JOURNAL   Biochem J 401 (1), 103-109 (2007)
   PUBMED   16939411
  REMARK    GeneRIF: This enzyme is a novel regulator of cardiolipin de novo
            biosynthesis and its resynthesis.
REFERENCE   8  (bases 1 to 1752)
  AUTHORS   Liu,J., Dai,Q., Chen,J., Durrant,D., Freeman,A., Liu,T.,
            Grossman,D. and Lee,R.M.
  TITLE     Phospholipid scramblase 3 controls mitochondrial structure,
            function, and apoptotic response
  JOURNAL   Mol Cancer Res 1 (12), 892-902 (2003)
   PUBMED   14573790
  REMARK    GeneRIF: PLS3 as a critical regulator of mitochondrial structure
            and respiration, and CL transport in apoptosis.
REFERENCE   9  (bases 1 to 1752)
  AUTHORS   Liu,J., Chen,J., Dai,Q. and Lee,R.M.
  TITLE     Phospholipid scramblase 3 is the mitochondrial target of protein
            kinase C delta-induced apoptosis
  JOURNAL   Cancer Res 63 (6), 1153-1156 (2003)
   PUBMED   12649167
  REMARK    GeneRIF: PLS3 is a downstream effector of PKC-delta in the
            mitochondria.
REFERENCE   10 (bases 1 to 1752)
  AUTHORS   Wiedmer,T., Zhou,Q., Kwoh,D.Y. and Sims,P.J.
  TITLE     Identification of three new members of the phospholipid scramblase
            gene family
  JOURNAL   Biochim Biophys Acta 1467 (1), 244-253 (2000)
   PUBMED   10930526
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC093026.1 and BC011735.2.
            
            On Nov 22, 2018 this sequence version replaced NM_020360.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Both variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.703258.1, BC093026.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000619711.5/ ENSP00000483743.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1746              BC093026.1         8-1753
            1747-1752           BC011735.2         1591-1596
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p13.1"
     gene            1..1752
                     /gene="PLSCR3"
                     /note="phospholipid scramblase 3"
                     /db_xref="GeneID:57048"
                     /db_xref="HGNC:HGNC:16495"
                     /db_xref="MIM:607611"
     exon            1..49
                     /gene="PLSCR3"
                     /inference="alignment:Splign:2.1.0"
     exon            50..213
                     /gene="PLSCR3"
                     /inference="alignment:Splign:2.1.0"
     CDS             207..1094
                     /gene="PLSCR3"
                     /note="isoform 1 is encoded by transcript variant 1; PL
                     scramblase 3; ca(2+)-dependent phospholipid scramblase 3"
                     /codon_start=1
                     /product="phospholipid scramblase 3 isoform 1"
                     /protein_id="NP_065093.2"
                     /db_xref="CCDS:CCDS42253.1"
                     /db_xref="GeneID:57048"
                     /db_xref="HGNC:HGNC:16495"
                     /db_xref="MIM:607611"
                     /translation="MAGYLPPKGYAPSPPPPYPVTPGYPEPALHPGPGQAPVPAQVPA
                     PAPGFALFPSPGPVALGSAAPFLPLPGVPSGLEFLVQIDQILIHQKAERVETFLGWET
                     CNRYELRSGAGQPLGQAAEESNCCARLCCGARRPLRVRLADPGDREVLRLLRPLHCGC
                     SCCPCGLQEMEVQAPPGTTIGHVLQTWHPFLPKFSIQDADRQTVLRVVGPCWTCGCGT
                     DTNFEVKTRDESRSVGRISKQWGGLVREALTDADDFGLQFPLDLDVRVKAVLLGATFL
                     IDYMFFEKRGGAGPSAVTS"
     exon            214..449
                     /gene="PLSCR3"
                     /inference="alignment:Splign:2.1.0"
     exon            450..492
                     /gene="PLSCR3"
                     /inference="alignment:Splign:2.1.0"
     exon            493..713
                     /gene="PLSCR3"
                     /inference="alignment:Splign:2.1.0"
     exon            714..875
                     /gene="PLSCR3"
                     /inference="alignment:Splign:2.1.0"
     exon            876..1037
                     /gene="PLSCR3"
                     /inference="alignment:Splign:2.1.0"
     exon            1038..1752
                     /gene="PLSCR3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1722..1727
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLSCR3"
                     /note="hexamer: TATAAA"
     polyA_site      1752
                     /gene="PLSCR3"
                     /note="major polyA site"
ORIGIN      
        1 cctgtgagct gccgagtgct aggcacccgg gctcttctgg gggctccaga ggcgccgccc
       61 aagagaccct gggccggcgc cgggcgcagc tgcctctccg tctttgtgtc tgtctctgtg
      121 tctgtctggc tatctccgag tttgcctccg cttccagaac taagccaccc agacaccatc
      181 atctcgaaaa ccccagccct tctcccatgg caggctactt gccccccaaa ggctacgccc
      241 cttcgccccc acctccctac cctgtcaccc ctgggtaccc ggagccggcg ctacatcctg
      301 ggcccgggca ggcgccagtg cccgcccagg tacctgcccc agctcccggc ttcgccctct
      361 tcccctcgcc tggccccgtg gccttggggt ctgctgcccc cttcttgcca ctgccagggg
      421 tgccttctgg cctcgaattc ctggtgcaga ttgatcagat tttgattcac cagaaggctg
      481 agcgagtgga aacgttccta ggctgggaga cctgtaatcg gtatgaactg cgctctgggg
      541 ccgggcagcc cctgggtcag gcggccgagg agagcaactg ctgcgcccgt ctgtgctgtg
      601 gcgcccgccg gccgctgcgt gtccgcctgg ccgaccccgg ggaccgtgag gtgctgcgtt
      661 tgctccgccc gctgcactgt ggctgcagct gctgcccctg tggcctccag gagatggaag
      721 tacaggctcc accaggcacc accattggcc acgtgctaca gacctggcat cccttcctcc
      781 ccaagttctc catccaggat gccgatcgcc agacagtctt gcgagtggtg gggccctgct
      841 ggacctgtgg ctgtggcaca gacaccaact ttgaggtgaa gactcgggat gaatcccgca
      901 gtgtgggccg catcagcaag cagtgggggg gcctggtccg agaagccctc acagatgcag
      961 atgactttgg cctacagttc ccgctggacc tggatgtgag ggtgaaggct gtgctgctgg
     1021 gagccacatt cctcattgac tacatgttct ttgagaagcg aggaggcgct gggccctctg
     1081 ccgtcaccag ttagaggcca ccatggtgtg aggagaccat cacctcgacc agaactccag
     1141 atggtcacct gccctggccc ctcctctggg cagccccttt cctccatgta cactgcaggg
     1201 gacagaaggg gggccccatc cctaccctac tccctggccg cctgcccctg tggttcccaa
     1261 ggaggggtat gtatgagagc cgctctcctg ctacctccca ccactgtccc agcagtccct
     1321 cggcacacag gcatatcagc tttcacactt tccccatgca ctctctccca cccccttcca
     1381 gggcctctgc tccaaaggag gcctctggaa cccaggactc tggggtttta caagagggct
     1441 ggggtgtgga agggcaagct gcaccaaaga cggtggatat agccaccgcc cccccgccgc
     1501 tgcctagcat ctgcttggcc aattagttca gcctcagacc atggcacttt gagggggtct
     1561 ctacctcccc atcaacagct gcagggggac cccagtgcca acttcctctc ccactagggc
     1621 cctgccttca gctggtgctt gctgcgattc ctgtgcctta tgtaactgcc cttccttccc
     1681 ttgccctagg aaaaaggctg catctttata tgttacattc atataaactt tgtaactttt
     1741 tggacattga aa
//
