LOCUS       NM_002669               3317 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens pleiotropic regulator 1 (PLRG1), transcript variant 1,
            mRNA.
ACCESSION   NM_002669
VERSION     NM_002669.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Kwon,J.H. and Kim,S.H.
  TITLE     YBX1 promotes epithelial-mesenchymal transition in hepatocellular
            carcinoma via transcriptional regulation of PLRG1
  JOURNAL   Med Oncol 41 (11), 280 (2024)
   PUBMED   39400789
  REMARK    GeneRIF: YBX1 promotes epithelial-mesenchymal transition in
            hepatocellular carcinoma via transcriptional regulation of PLRG1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Liu,Z., Ye,Y., Liu,Y., Liu,Y., Chen,H., Shen,M., Wang,Z., Huang,S.,
            Han,L., Chen,Z. and He,X.
  TITLE     RNA Helicase DHX37 Facilitates Liver Cancer Progression by
            Cooperating with PLRG1 to Drive Superenhancer-Mediated
            Transcription of Cyclin D1
  JOURNAL   Cancer Res 82 (10), 1937-1952 (2022)
   PUBMED   35290436
  REMARK    GeneRIF: RNA Helicase DHX37 Facilitates Liver Cancer Progression by
            Cooperating with PLRG1 to Drive Superenhancer-Mediated
            Transcription of Cyclin D1.
REFERENCE   3  (bases 1 to 3317)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 3317)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 3317)
  AUTHORS   Ajuh,P. and Lamond,A.I.
  TITLE     Identification of peptide inhibitors of pre-mRNA splicing derived
            from the essential interaction domains of CDC5L and PLRG1
  JOURNAL   Nucleic Acids Res 31 (21), 6104-6116 (2003)
   PUBMED   14576297
  REMARK    GeneRIF: the interaction between CDC5L and PLRG1 is essential for
            pre-mRNA splicing
            Erratum:[Nucleic Acids Res. 2022 Aug 12;50(14):8400. doi:
            10.1093/nar/gkac623.. PMID: 35848918]
REFERENCE   6  (bases 1 to 3317)
  AUTHORS   Jurica,M.S., Licklider,L.J., Gygi,S.R., Grigorieff,N. and
            Moore,M.J.
  TITLE     Purification and characterization of native spliceosomes suitable
            for three-dimensional structural analysis
  JOURNAL   RNA 8 (4), 426-439 (2002)
   PUBMED   11991638
REFERENCE   7  (bases 1 to 3317)
  AUTHORS   Ajuh,P., Sleeman,J., Chusainow,J. and Lamond,A.I.
  TITLE     A direct interaction between the carboxyl-terminal region of CDC5L
            and the WD40 domain of PLRG1 is essential for pre-mRNA splicing
  JOURNAL   J Biol Chem 276 (45), 42370-42381 (2001)
   PUBMED   11544257
REFERENCE   8  (bases 1 to 3317)
  AUTHORS   Ajuh,P., Kuster,B., Panov,K., Zomerdijk,J.C., Mann,M. and
            Lamond,A.I.
  TITLE     Functional analysis of the human CDC5L complex and identification
            of its components by mass spectrometry
  JOURNAL   EMBO J 19 (23), 6569-6581 (2000)
   PUBMED   11101529
REFERENCE   9  (bases 1 to 3317)
  AUTHORS   Nemeth,K., Salchert,K., Putnoky,P., Bhalerao,R., Koncz-Kalman,Z.,
            Stankovic-Stangeland,B., Bako,L., Mathur,J., Okresz,L., Stabel,S.,
            Geigenberger,P., Stitt,M., Redei,G.P., Schell,J. and Koncz,C.
  TITLE     Pleiotropic control of glucose and hormone responses by PRL1, a
            nuclear WD protein, in Arabidopsis
  JOURNAL   Genes Dev 12 (19), 3059-3073 (1998)
   PUBMED   9765207
  REMARK    GeneRIF: Study includes the isolation of a cDNA encoding the human
            PRL1 homolog.
REFERENCE   10 (bases 1 to 3317)
  AUTHORS   Neubauer,G., King,A., Rappsilber,J., Calvio,C., Watson,M., Ajuh,P.,
            Sleeman,J., Lamond,A. and Mann,M.
  TITLE     Mass spectrometry and EST-database searching allows
            characterization of the multi-protein spliceosome complex
  JOURNAL   Nat Genet 20 (1), 46-50 (1998)
   PUBMED   9731529
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP314297.1, BC106004.1,
            AC107385.4 and AW291931.1.
            
            On Nov 22, 2018 this sequence version replaced NM_002669.3.
            
            Summary: This gene encodes a core component of the cell division
            cycle 5-like (CDC5L) complex. The CDC5L complex is part of the
            spliceosome and is required for pre-mRNA splicing. The encoded
            protein plays a critical role in alternative splice site selection.
            Alternatively spliced transcript variants encoding multiple
            isoforms have been observed for this gene. [provided by RefSeq, Jan
            2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC036046.1, SRR11853561.22706.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000499023.7/ ENSP00000424417.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-29                BP314297.1         34-62
            30-1812             BC106004.1         1-1783
            1813-2981           AC107385.4         11079-12247         c
            2982-3317           AW291931.1         25-360              c
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q31.3"
     gene            1..3317
                     /gene="PLRG1"
                     /gene_synonym="Cwc1; PRL1; PRP46; PRPF46; TANGO4"
                     /note="pleiotropic regulator 1"
                     /db_xref="GeneID:5356"
                     /db_xref="HGNC:HGNC:9089"
                     /db_xref="MIM:605961"
     exon            1..101
                     /gene="PLRG1"
                     /gene_synonym="Cwc1; PRL1; PRP46; PRPF46; TANGO4"
                     /inference="alignment:Splign:2.1.0"
     CDS             93..1637
                     /gene="PLRG1"
                     /gene_synonym="Cwc1; PRL1; PRP46; PRPF46; TANGO4"
                     /note="isoform 1 is encoded by transcript variant 1;
                     pleiotropic regulator 1 (PRL1 homolog, Arabidopsis);
                     transport and golgi organization 4 homolog"
                     /codon_start=1
                     /product="pleiotropic regulator 1 isoform 1"
                     /protein_id="NP_002660.1"
                     /db_xref="CCDS:CCDS34083.1"
                     /db_xref="GeneID:5356"
                     /db_xref="HGNC:HGNC:9089"
                     /db_xref="MIM:605961"
                     /translation="MVEEVQKHSVHTLVFRSLKRTHDMFVADNGKPVPLDEESHKRKM
                     AIKLRNEYGPVLHMPTSKENLKEKGPQNATDSYVHKQYPANQGQEVEYFVAGTHPYPP
                     GPGVALTADTKIQRMPSESAAQSLAVALPLQTKADANRTAPSGSEYRHPGASDRPQPT
                     AMNSIVMETGNTKNSALMAKKAPTMPKPQWHPPWKLYRVISGHLGWVRCIAVEPGNQW
                     FVTGSADRTIKIWDLASGKLKLSLTGHISTVRGVIVSTRSPYLFSCGEDKQVKCWDLE
                     YNKVIRHYHGHLSAVYGLDLHPTIDVLVTCSRDSTARIWDVRTKASVHTLSGHTNAVA
                     TVRCQAAEPQIITGSHDTTIRLWDLVAGKTRVTLTNHKKSVRAVVLHPRHYTFASGSP
                     DNIKQWKFPDGSFIQNLSGHNAIINTLTVNSDGVLVSGADNGTMHLWDWRTGYNFQRV
                     HAAVQPGSLDSESGIFACAFDQSESRLLTAEADKTIKVYREDDTATEETHPVSWKPEI
                     IKRKRF"
     exon            102..208
                     /gene="PLRG1"
                     /gene_synonym="Cwc1; PRL1; PRP46; PRPF46; TANGO4"
                     /inference="alignment:Splign:2.1.0"
     exon            209..351
                     /gene="PLRG1"
                     /gene_synonym="Cwc1; PRL1; PRP46; PRPF46; TANGO4"
                     /inference="alignment:Splign:2.1.0"
     exon            352..405
                     /gene="PLRG1"
                     /gene_synonym="Cwc1; PRL1; PRP46; PRPF46; TANGO4"
                     /inference="alignment:Splign:2.1.0"
     exon            406..496
                     /gene="PLRG1"
                     /gene_synonym="Cwc1; PRL1; PRP46; PRPF46; TANGO4"
                     /inference="alignment:Splign:2.1.0"
     exon            497..584
                     /gene="PLRG1"
                     /gene_synonym="Cwc1; PRL1; PRP46; PRPF46; TANGO4"
                     /inference="alignment:Splign:2.1.0"
     exon            585..686
                     /gene="PLRG1"
                     /gene_synonym="Cwc1; PRL1; PRP46; PRPF46; TANGO4"
                     /inference="alignment:Splign:2.1.0"
     exon            687..779
                     /gene="PLRG1"
                     /gene_synonym="Cwc1; PRL1; PRP46; PRPF46; TANGO4"
                     /inference="alignment:Splign:2.1.0"
     exon            780..929
                     /gene="PLRG1"
                     /gene_synonym="Cwc1; PRL1; PRP46; PRPF46; TANGO4"
                     /inference="alignment:Splign:2.1.0"
     exon            930..1031
                     /gene="PLRG1"
                     /gene_synonym="Cwc1; PRL1; PRP46; PRPF46; TANGO4"
                     /inference="alignment:Splign:2.1.0"
     exon            1032..1134
                     /gene="PLRG1"
                     /gene_synonym="Cwc1; PRL1; PRP46; PRPF46; TANGO4"
                     /inference="alignment:Splign:2.1.0"
     exon            1135..1243
                     /gene="PLRG1"
                     /gene_synonym="Cwc1; PRL1; PRP46; PRPF46; TANGO4"
                     /inference="alignment:Splign:2.1.0"
     exon            1244..1383
                     /gene="PLRG1"
                     /gene_synonym="Cwc1; PRL1; PRP46; PRPF46; TANGO4"
                     /inference="alignment:Splign:2.1.0"
     exon            1384..1577
                     /gene="PLRG1"
                     /gene_synonym="Cwc1; PRL1; PRP46; PRPF46; TANGO4"
                     /inference="alignment:Splign:2.1.0"
     exon            1578..3317
                     /gene="PLRG1"
                     /gene_synonym="Cwc1; PRL1; PRP46; PRPF46; TANGO4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1790..1795
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLRG1"
                     /gene_synonym="Cwc1; PRL1; PRP46; PRPF46; TANGO4"
                     /note="hexamer: AATAAA"
     polyA_site      1812
                     /gene="PLRG1"
                     /gene_synonym="Cwc1; PRL1; PRP46; PRPF46; TANGO4"
                     /note="major polyA site"
     regulatory      2040..2045
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLRG1"
                     /gene_synonym="Cwc1; PRL1; PRP46; PRPF46; TANGO4"
                     /note="hexamer: ATTAAA"
     polyA_site      2061
                     /gene="PLRG1"
                     /gene_synonym="Cwc1; PRL1; PRP46; PRPF46; TANGO4"
     regulatory      3297..3302
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLRG1"
                     /gene_synonym="Cwc1; PRL1; PRP46; PRPF46; TANGO4"
                     /note="hexamer: AATAAA"
     polyA_site      3317
                     /gene="PLRG1"
                     /gene_synonym="Cwc1; PRL1; PRP46; PRPF46; TANGO4"
ORIGIN      
        1 aattccggaa ggtgctgcac agctgtggcg gcgggtactg cgttagtgat tagagtttct
       61 tccctgccgg aggtgggata cacggtagca tcatggtcga ggaggtacag aaacattctg
      121 tacacaccct tgtgttcagg tcgttgaaga ggacccatga catgtttgta gctgataatg
      181 gaaaacctgt gcctttagat gaagagagtc acaaacgaaa aatggcaatc aagcttcgta
      241 atgagtatgg tcctgtgttg catatgccta cttcaaaaga aaatcttaaa gagaagggtc
      301 ctcagaatgc aacggattca tatgttcata aacagtaccc tgccaatcaa ggacaagaag
      361 ttgaatactt tgtggcaggt acacatccat acccaccagg acctggggtt gctttgacag
      421 cagatactaa gatccagaga atgccaagtg aatcagctgc acagtcctta gcggtggcat
      481 tacctttgca gaccaaggct gatgcaaatc gtactgcccc tagtggaagt gaataccgac
      541 atcctggggc ttctgaccgt ccacagccta cagcgatgaa ttcaattgtc atggagactg
      601 gcaataccaa gaactctgca ctgatggcta aaaaagcccc tacaatgcca aaaccccagt
      661 ggcacccacc gtggaaactc tacagggtta tcagtgggca tcttggctgg gttcgatgta
      721 ttgctgtgga acctggaaat cagtggtttg ttactggatc tgctgacaga actataaaga
      781 tctgggactt ggctagtggc aaattaaaac tgtcattgac tgggcatatt agtactgtgc
      841 ggggcgtgat agtaagcaca aggagcccat atctgttctc ttgtggagaa gacaaacaag
      901 tgaaatgctg ggatctcgaa tacaataagg ttatacggca ttatcatgga catttaagtg
      961 cagtgtatgg tttggatttg cacccgacaa tcgatgtgtt ggtaacctgt agtcgagatt
     1021 caactgcacg gatttgggat gtgagaacta aagccagtgt acacacatta tctggacata
     1081 caaatgcagt tgctacagtg agatgtcagg ctgcagaacc acaaattatt acaggaagcc
     1141 atgatactac aattcgatta tgggatctgg tggctggaaa aacaagagtg acattaacaa
     1201 atcacaaaaa atcagttagg gctgtggttt tacatccaag acattacaca tttgcatctg
     1261 gttctccaga taacataaag cagtggaaat tccctgatgg aagtttcatt caaaatcttt
     1321 ccggtcataa tgctattatt aacacattga cggtaaattc tgatggagtg cttgtatctg
     1381 gagctgacaa tggcaccatg catctttggg actggagaac tggctacaat tttcagagag
     1441 ttcacgcagc tgtgcaacct gggtctttgg acagtgaatc aggaatattt gcttgtgctt
     1501 ttgatcagtc tgaaagtcga ttactaacag ctgaagctga taaaaccatt aaagtataca
     1561 gagaggatga cacagccaca gaagaaactc atccagtcag ctggaaacca gaaattatca
     1621 agagaaagag attttaatga atgtggaatt ttttctctct cttttttttt ctttttaatt
     1681 aaaaaaaaaa aagcttggcg ttcatgagga tatccagtca ttttgtgctc tggctgggaa
     1741 tataaaggag aaattcactt gcttcaatca ttgctgcttc atattttaca ataaactgtc
     1801 ccctgtcccc tacccctgtg tttttatttc taaaacaatt tgtgatacta ggaagatgca
     1861 gatatcaagt aaatgcaggt ttattgaaca taactattct agatgtaata ttttgacagt
     1921 cttattagaa aacccccttt tttaaaaaaa atgtatgaaa ccgataatta ggacatttga
     1981 aggagcagta gttttttttt agtcctttaa aaaataacat ctgtgactaa taatttgaga
     2041 ttaaaagatt tgaagtctta atttatgtat gtttattcca aaaacagttt ttgagtgtat
     2101 gttctacaaa gttctgcact agatactgtt caggaaatag acaagtaaga tgtgtctatc
     2161 ctctaagact tccagtttgg tagcttagta tagaaattct gtaacagagg tgcaggtgaa
     2221 atgctggggg aacagaaggg ataagaccat ttatttttct caggaaggat tgtggaagac
     2281 ttcatagagt aagtggcatt ggagattacc ttgaagtgaa ggaagacaaa gaaggaaaag
     2341 agatgaggca taaagtgatg aaaccaggca caagaaagtg tgcggtgtgt tctgggacaa
     2401 agaatgttct gctactgtcg aataatggga ctaaagctgg cttgttctgg ggaaagctca
     2461 tataagtatg gattttattc ctcaactagt aggataccaa tactggtatt gaaacttggg
     2521 gaaaataact ggagatacca gtgcagctat ttaaagctgt agcaagggct gcaatcttgc
     2581 ggagatttta aagagaagtt ttaaagtttc taatactgat gcctcttttt ggtaaataca
     2641 agttttataa atcctgccct gggatcctga ttccccatta atcaagattt gtcagacttc
     2701 accttctata attagaaaac acagttataa gaacagtcaa ttttttaaat tttccaaatt
     2761 aaaaaattgc accatgattt tgaacaagca cttccaatta cattacccat cttgtatgcc
     2821 ataggtggga gtataattgt cacagccttt aggaatgtag ttttctggga tttattgaaa
     2881 ctttgaacct tttggcctac taagttcatt cctaggaaac tgcctaatgg gaatgatctg
     2941 acaagtgtac acaagcaaag tcattgcacc tttgttcttt aatacttaaa actagcccaa
     3001 atgccctgtc agtaagggac tggtttaata gatggtacct ttatgcaatt tgtttctaag
     3061 tattcgttaa gagagtgagg aatgtctgga ttattagggc aagatttcta aacttgggta
     3121 aaaaaaaaag ttgtgcaagc atttttgtgg acaacttgat gctctaaaat ataagtaaca
     3181 ccacattact aacagtggct ttgttaggaa gcagaattat ggaagaactt tcatgggtgg
     3241 ctttacatat ttctataatg ttttaatttt ctataagcat gtatttcgta aacttaaata
     3301 aagaaaaaag aacacta
//
