LOCUS       NM_001367868            6502 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens perilipin 4 (PLIN4), transcript variant 4, mRNA.
ACCESSION   NM_001367868 XM_006722868
VERSION     NM_001367868.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   Maggi,L., Gibertini,S., Iannibelli,E., Gallone,A., Bonanno,S.,
            Cazzato,D., Gerevini,S., Moscatelli,M., Blasevich,F., Riolo,G.,
            Mantegazza,R. and Ruggieri,A.
  TITLE     PLIN4-related myopathy: clinical, histological and imaging data in
            a large cohort of patients
  JOURNAL   J Neurol 270 (9), 4538-4543 (2023)
   PUBMED   37145156
  REMARK    GeneRIF: PLIN4-related myopathy: clinical, histological and imaging
            data in a large cohort of patients.
REFERENCE   2  (bases 1 to 6502)
  AUTHORS   Gimenez-Andres,M., Emersic,T., Antoine-Bally,S., D'Ambrosio,J.M.,
            Antonny,B., Derganc,J. and Copic,A.
  TITLE     Exceptional stability of a perilipin on lipid droplets depends on
            its polar residues, suggesting multimeric assembly
  JOURNAL   Elife 10, e61401 (2021)
   PUBMED   33856341
  REMARK    GeneRIF: Exceptional stability of a perilipin on lipid droplets
            depends on its polar residues, suggesting multimeric assembly.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6502)
  AUTHORS   Ruggieri,A., Naumenko,S., Smith,M.A., Iannibelli,E., Blasevich,F.,
            Bragato,C., Gibertini,S., Barton,K., Vorgerd,M., Marcus,K.,
            Wang,P., Maggi,L., Mantegazza,R., Dowling,J.J., Kley,R.A., Mora,M.
            and Minassian,B.A.
  TITLE     Multiomic elucidation of a coding 99-mer repeat-expansion skeletal
            muscle disease
  JOURNAL   Acta Neuropathol 140 (2), 231-235 (2020)
   PUBMED   32451610
  REMARK    GeneRIF: Multiomic elucidation of a coding 99-mer repeat-expansion
            skeletal muscle disease.
REFERENCE   4  (bases 1 to 6502)
  AUTHORS   Sirois,I., Aguilar-Mahecha,A., Lafleur,J., Fowler,E., Vu,V.,
            Scriver,M., Buchanan,M., Chabot,C., Ramanathan,A., Balachandran,B.,
            Legare,S., Przybytkowski,E., Lan,C., Krzemien,U., Cavallone,L.,
            Aleynikova,O., Ferrario,C., Guilbert,M.C., Benlimame,N., Saad,A.,
            Alaoui-Jamali,M., Saragovi,H.U., Josephy,S., O'Flanagan,C.,
            Hursting,S.D., Richard,V.R., Zahedi,R.P., Borchers,C.H., Bareke,E.,
            Nabavi,S., Tonellato,P., Roy,J.A., Robidoux,A., Marcus,E.A.,
            Mihalcioiu,C., Majewski,J. and Basik,M.
  TITLE     A Unique Morphological Phenotype in Chemoresistant Triple-Negative
            Breast Cancer Reveals Metabolic Reprogramming and PLIN4 Expression
            as a Molecular Vulnerability
  JOURNAL   Mol Cancer Res 17 (12), 2492-2507 (2019)
   PUBMED   31537618
  REMARK    GeneRIF: These findings underlie the importance of a novel
            morphologic-metabolic phenotype associated with chemotherapy
            resistance in triple-negative breast cancer , and bring to light
            novel therapeutic targets resulting from vulnerabilities in this
            phenotype, including the expression of PLIN4 essential for
            stabilizing lipid droplets in resistant cells.
REFERENCE   5  (bases 1 to 6502)
  AUTHORS   Rodrigues,D.M., Manfro,G.G., Levitan,R.D., Steiner,M., Meaney,M.J.
            and Silveira,P.P.
  TITLE     Moderating effect of PLIN4 genetic variant on impulsivity traits in
            5-year-old-children born small for gestational age
  JOURNAL   Prostaglandins Leukot Essent Fatty Acids 137, 19-25 (2018)
   PUBMED   30293593
  REMARK    GeneRIF: We investigated whether the presence of the A allele of
            rs8887 SNP (PLIN4 gene), known to be associated with increased
            sensitivity to the consumption of n-3 PUFAs, interacts with fetal
            growth influencing inhibitory control.  There was a significant
            interaction between birth weight and the presence of the A allele
            on SSRT performance,in which lower birth weight associated with
            poorer inhibitory control only in non-carrier
REFERENCE   6  (bases 1 to 6502)
  AUTHORS   Moessinger,C., Kuerschner,L., Spandl,J., Shevchenko,A. and
            Thiele,C.
  TITLE     Human lysophosphatidylcholine acyltransferases 1 and 2 are located
            in lipid droplets where they catalyze the formation of
            phosphatidylcholine
  JOURNAL   J Biol Chem 286 (24), 21330-21339 (2011)
   PUBMED   21498505
REFERENCE   7  (bases 1 to 6502)
  AUTHORS   Kimmel,A.R., Brasaemle,D.L., McAndrews-Hill,M., Sztalryd,C. and
            Londos,C.
  TITLE     Adoption of PERILIPIN as a unifying nomenclature for the mammalian
            PAT-family of intracellular lipid storage droplet proteins
  JOURNAL   J Lipid Res 51 (3), 468-471 (2010)
   PUBMED   19638644
  REMARK    Review article
REFERENCE   8  (bases 1 to 6502)
  AUTHORS   Sone,Y., Yamaguchi,K., Fujiwara,A., Kido,T., Kawahara,K.,
            Ishiwaki,A., Kondo,K., Morita,Y., Tominaga,N. and Otsuka,Y.
  TITLE     Association of lifestyle factors, polymorphisms in adiponectin,
            perilipin and hormone sensitive lipase, and clinical markers in
            Japanese males
  JOURNAL   J Nutr Sci Vitaminol (Tokyo) 56 (2), 123-131 (2010)
   PUBMED   20495294
  REMARK    GeneRIF: Those findings indicate improvement and conservation of
            lifestyle depending on genetic predisposition in ADIPOQ, PLIN and
            LIPE should be encouraged.
            GeneRIF: Observational study of gene-disease association and
            gene-environment interaction. (HuGE Navigator)
REFERENCE   9  (bases 1 to 6502)
  AUTHORS   Wolins,N.E., Skinner,J.R., Schoenfish,M.J., Tzekov,A., Bensch,K.G.
            and Bickel,P.E.
  TITLE     Adipocyte protein S3-12 coats nascent lipid droplets
  JOURNAL   J Biol Chem 278 (39), 37713-37721 (2003)
   PUBMED   12840023
  REMARK    GeneRIF: Characterization of homologous mouse gene product, plasma
            membrane associated protein, S3-12.
REFERENCE   10 (bases 1 to 6502)
  AUTHORS   Sangiuolo,F., Bruscia,E., Capon,F., Servidei,S., Dallapiccola,B.
            and Novelli,G.
  TITLE     Fine mapping of a distinctive autosomal dominant vacuolar
            neuromyopathy using 11 novel microsatellite markers from chromosome
            band 19p13.3
  JOURNAL   Eur J Hum Genet 8 (10), 809-812 (2000)
   PUBMED   11039585
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC011498.7.
            
            On Jun 2, 2019 this sequence version replaced NM_001367868.1.
            
            Summary: Members of the perilipin family, such as PLIN4, coat
            intracellular lipid storage droplets (Wolins et al., 2003 [PubMed
            12840023]).[supplied by OMIM, Feb 2010].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA2142680,
                              SAMEA2153980 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000301286.5/ ENSP00000301286.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-102               AC011498.7         150038-150139       c
            103-170             AC011498.7         149875-149942       c
            171-315             AC011498.7         149207-149351       c
            316-377             AC011498.7         148270-148331       c
            378-3633            AC011498.7         142099-145354       c
            3634-3821           AC011498.7         140421-140608       c
            3822-3908           AC011498.7         136514-136600       c
            3909-6502           AC011498.7         133845-136438       c
FEATURES             Location/Qualifiers
     source          1..6502
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.3"
     gene            1..6502
                     /gene="PLIN4"
                     /gene_synonym="KIAA1881; MDRV; MRUPAV; NIPA2; S3-12"
                     /note="perilipin 4"
                     /db_xref="GeneID:729359"
                     /db_xref="HGNC:HGNC:29393"
                     /db_xref="MIM:613247"
     exon            1..102
                     /gene="PLIN4"
                     /gene_synonym="KIAA1881; MDRV; MRUPAV; NIPA2; S3-12"
                     /inference="alignment:Splign:2.1.0"
     exon            103..170
                     /gene="PLIN4"
                     /gene_synonym="KIAA1881; MDRV; MRUPAV; NIPA2; S3-12"
                     /inference="alignment:Splign:2.1.0"
     CDS             120..4235
                     /gene="PLIN4"
                     /gene_synonym="KIAA1881; MDRV; MRUPAV; NIPA2; S3-12"
                     /note="isoform 2 is encoded by transcript variant 4;
                     adipocyte protein S3-12; muscular dystrophy, with rimmed
                     vacuoles"
                     /codon_start=1
                     /product="perilipin-4 isoform 2"
                     /protein_id="NP_001354797.1"
                     /db_xref="CCDS:CCDS92489.1"
                     /db_xref="GeneID:729359"
                     /db_xref="HGNC:HGNC:29393"
                     /db_xref="MIM:613247"
                     /translation="MSAPDEGRRDPPKPKGKTLGSFFGSLPGFSSARNLVANAHSSAR
                     ARPAADPTGAPAAEAAQPQAQVAAHPEQTAPWTEKELQPSEKMVSGAKDLVCSKMSRA
                     KDAVSSGVASVVDVAKGVVQGGLDTTRSALTGTKEVVSSGVTGAMDMAKGAVQGGLDT
                     SKAVLTGTKDTVSTGLTGAVNVAKGTVQAGVDTTKTVLTGTKDTVTTGVMGAVNLAKG
                     TVQTGVETSKAVLTGTKDAVSTGLTGAVNVARGSIQTGVDTSKTVLTGTKDTVCSGVT
                     GAMNVAKGTIQTGVDTSKTVLTGTKDTVCSGVTGAMNVAKGTIQTGVDTSKTVLTGTK
                     DTVCSGVTGAMNVAKGTIQTGVDTTKTVLTGTKNTVCSGVTGAVNLAKEAIQGGLDTT
                     KSMVMGTKDTMSTGLTGAANVAKGAMQTGLNTTQNIATGTKDTVCSGVTGAMNLARGT
                     IQTGVDTTKIVLTGTKDTVCSGVTGAANVAKGAVQGGLDTTKSVLTGTKDAVSTGLTG
                     AVNVAKGTVQTGVDTTKTVLTGTKDTVCSGVTSAVNVAKGAVQGGLDTTKSVVIGTKD
                     TMSTGLTGAANVAKGAVQTGVDTAKTVLTGTKDTVTTGLVGAVNVAKGTVQTGMDTTK
                     TVLTGTKDTIYSGVTSAVNVAKGAVQTGLKTTQNIATGTKNTFGSGVTSAVNVAKGAA
                     QTGVDTAKTVLTGTKDTVTTGLMGAVNVAKGTVQTSVDTTKTVLTGTKDTVCSGVTGA
                     ANVAKGAIQGGLDTTKSVLTGTKDAVSTGLTGAVKLAKGTVQTGMDTTKTVLTGTKDA
                     VCSGVTGAANVAKGAVQMGVDTAKTVLTGTKDTVCSGVTGAANVAKGAVQTGLKTTQN
                     IATGTKNTLGSGVTGAAKVAKGAVQGGLDTTKSVLTGTKDAVSTGLTGAVNLAKGTVQ
                     TGVDTSKTVLTGTKDTVCSGVTGAVNVAKGTVQTGVDTAKTVLSGAKDAVTTGVTGAV
                     NVAKGTVQTGVDASKAVLMGTKDTVFSGVTGAMSMAKGAVQGGLDTTKTVLTGTKDAV
                     SAGLMGSGNVATGATHTGLSTFQNWLPSTPATSWGGLTSSRTTDNGGEQTALSPQEAP
                     FSGISTPPDVLSVGPEPAWEAAATTKGLATDVATFTQGAAPGREDTGLLATTHGPEEA
                     PRLAMLQNELEGLGDIFHPMNAEEQAQLAASQPGPKVLSAEQGSYFVRLGDLGPSFRQ
                     RAFEHAVSHLQHGQFQARDTLAQLQDCFRLIEKAQQAPEGQPRLDQGSGASAEDAAVQ
                     EERDAGVLSRVCGLLRQLHTAYSGLVSSLQGLPAELQQPVGRARHSLCELYGIVASAG
                     SVEELPAERLVQSREGVHQAWQGLEQLLEGLQHNPPLSWLVGPFALPAGGQ"
     exon            171..315
                     /gene="PLIN4"
                     /gene_synonym="KIAA1881; MDRV; MRUPAV; NIPA2; S3-12"
                     /inference="alignment:Splign:2.1.0"
     exon            316..377
                     /gene="PLIN4"
                     /gene_synonym="KIAA1881; MDRV; MRUPAV; NIPA2; S3-12"
                     /inference="alignment:Splign:2.1.0"
     exon            378..3633
                     /gene="PLIN4"
                     /gene_synonym="KIAA1881; MDRV; MRUPAV; NIPA2; S3-12"
                     /inference="alignment:Splign:2.1.0"
     exon            3634..3821
                     /gene="PLIN4"
                     /gene_synonym="KIAA1881; MDRV; MRUPAV; NIPA2; S3-12"
                     /inference="alignment:Splign:2.1.0"
     exon            3822..3908
                     /gene="PLIN4"
                     /gene_synonym="KIAA1881; MDRV; MRUPAV; NIPA2; S3-12"
                     /inference="alignment:Splign:2.1.0"
     exon            3909..6502
                     /gene="PLIN4"
                     /gene_synonym="KIAA1881; MDRV; MRUPAV; NIPA2; S3-12"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6472..6477
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLIN4"
                     /gene_synonym="KIAA1881; MDRV; MRUPAV; NIPA2; S3-12"
                     /note="hexamer: AATAAA"
     polyA_site      6502
                     /gene="PLIN4"
                     /gene_synonym="KIAA1881; MDRV; MRUPAV; NIPA2; S3-12"
                     /note="major polyA site"
ORIGIN      
        1 agctgccagc cgggccggtc cgtcctccag gggaccccgc gcagtccagg tgagcggggc
       61 cgcgtctgct tcggtgacac cagggtgagg ccaggcctgc agcttctcac gttctcacta
      121 tgtctgctcc agacgaaggg agacgggatc cccccaaacc gaagggcaag accctgggca
      181 gcttctttgg gtccctgcct ggcttcagct ctgcccggaa cctggtggcc aacgcacata
      241 gctcggcgag agcccggccg gccgctgacc ccacaggagc gcctgctgcc gaggctgccc
      301 aaccacaggc tcaggtggct gcccacccag agcagacggc cccatggacg gagaaggagc
      361 tgcaaccttc ggaaaagatg gtgtccgggg ccaaagacct ggtgtgttcc aagatgtcca
      421 gggccaagga tgccgtgtcc tccggggtgg ccagcgtggt ggacgtggct aagggagtgg
      481 tccagggagg cctggacacc actcggtctg cacttacggg caccaaggag gtggtgtcca
      541 gcggggtcac aggggccatg gacatggcta agggggccgt ccaagggggt ctggacacct
      601 cgaaggctgt cctcaccggc accaaggaca cggtgtccac tgggctcacg ggggcagtga
      661 atgtggccaa agggaccgta caggccggtg tggacaccac caagactgtg ctgaccggca
      721 ccaaagacac agtgactact ggggtcatgg gggcagtgaa cttggccaaa gggactgtcc
      781 agactggcgt ggaaacctcc aaggctgtgc tgaccggcac caaagatgct gtgtccactg
      841 ggctcacagg ggcagtgaat gtggccagag gaagcattca gaccggtgtg gacaccagta
      901 agactgtcct aacaggtacc aaggacaccg tctgtagtgg ggtgactggt gccatgaatg
      961 tggccaaagg aaccatccag accggcgtgg acaccagtaa gactgtccta acaggtacca
     1021 aggacaccgt ctgtagtggg gtgactggtg ccatgaatgt ggccaaagga accatccaga
     1081 ccggcgtgga caccagtaag actgtcctaa caggtaccaa ggacaccgtc tgtagtgggg
     1141 tgactggtgc catgaatgtg gccaaaggaa ccatccagac cggcgtggac accaccaaga
     1201 ctgtcctaac tggcaccaag aacactgtct gcagtggggt gaccggtgcc gtgaacttgg
     1261 ccaaagaggc catccagggg ggcctggata ccaccaagtc tatggtcatg ggtacgaaag
     1321 acacgatgtc cactgggctc acaggggcag cgaatgtggc caagggggcc atgcaaactg
     1381 ggctgaacac aacccaaaat atcgcaacag gtacaaagga caccgtctgc agtggggtga
     1441 ctggtgccat gaatttggcc agaggaacca tccagacagg cgtggacacc accaagatcg
     1501 ttctaactgg taccaaggac actgtctgca gtggggtcac cggtgctgcg aatgtggcca
     1561 aaggggccgt ccagggcggc ctggacacta caaagtctgt cctgactggc actaaagatg
     1621 ctgtgtccac tgggctcaca ggggctgtga acgtggccaa agggaccgtc cagaccggcg
     1681 tagacaccac caagactgtc ctaaccggca ccaaggacac cgtctgcagt ggggtgacca
     1741 gtgctgtgaa cgtggccaaa ggggccgtcc aggggggcct ggacaccacc aagtctgtgg
     1801 tcataggtac aaaagacacg atgtccactg ggctcacggg ggcagcgaat gtggccaagg
     1861 gggctgtcca gacaggtgta gacacagcca agaccgtgct gaccggcacc aaggacacag
     1921 tgactactgg gctcgtgggg gcagtgaatg tcgccaaagg gaccgtccag acaggcatgg
     1981 acaccaccaa aactgtccta accggtacca aggacaccat ctacagtggg gtcaccagtg
     2041 ccgtgaacgt ggccaagggg gctgtgcaaa ctgggctgaa aacgacccaa aatatcgcga
     2101 caggtacaaa gaacaccttt ggcagtgggg tgaccagtgc tgtgaatgtg gccaaagggg
     2161 ctgcccagac aggtgtagac acggccaaga ccgtgctgac cggcaccaag gacacagtca
     2221 ctactgggct catgggggca gtgaatgtcg ccaaagggac tgtccagacc agtgtggaca
     2281 ccaccaagac tgtcctaact ggtaccaagg acaccgtctg cagtggggtg accggtgctg
     2341 cgaatgtggc caaaggggcc atccaagggg gcctggacac tacaaagtct gtcctgactg
     2401 gcactaaaga tgctgtgtcc actgggctca caggggctgt gaagttggcc aaagggactg
     2461 tccagaccgg catggacacc accaagactg tgttaactgg taccaaggat gctgtgtgca
     2521 gtggggtgac cggtgctgcg aatgtggcca agggggccgt ccagatgggt gtagacacgg
     2581 ccaagaccgt gctgaccggt accaaggaca ctgtctgcag tggggtcacc ggtgctgcga
     2641 acgtggccaa gggtgctgtg caaactgggc tgaaaacgac ccaaaatatc gcaacaggta
     2701 caaagaacac ccttggcagt ggggtgaccg gtgctgcgaa agtggccaaa ggggccgtcc
     2761 aggggggcct ggacactaca aagtctgtcc tgactggcac taaagatgcc gtgtccactg
     2821 ggctcacagg ggctgtgaac ttggccaaag ggactgtcca gaccggcgtg gacaccagca
     2881 agactgtcct gaccggtacc aaggacaccg tctgcagtgg agtcactggt gccgtaaatg
     2941 tggccaaagg gaccgtccag acaggtgtgg acacagccaa gacggtgctg agtggcgcta
     3001 aggatgcagt gactactgga gtcacggggg cagtgaatgt ggccaaagga accgtgcaga
     3061 ccggcgtgga cgcctccaag gctgtgctta tgggtaccaa ggacactgtc ttcagtgggg
     3121 ttaccggtgc catgagcatg gccaaagggg ccgtccaggg gggcctggac accaccaaga
     3181 cagtgctgac cggaaccaaa gacgcagtgt ccgctgggct catggggtca gggaacgtgg
     3241 cgacaggggc cacccacact ggcctcagca ccttccagaa ctggttacct agtacccccg
     3301 ccacctcctg gggtggactc accagttcca ggaccacaga caatggtggg gagcagactg
     3361 ccctgagccc ccaagaggcc ccgttctctg gcatctccac gcccccggat gtgctcagtg
     3421 taggcccgga gcctgcctgg gaagccgcag ccactaccaa gggccttgcg actgacgtgg
     3481 cgacgttcac ccaaggggcc gccccaggca gggaggacac ggggcttttg gccaccacac
     3541 acggccccga agaagcccca cgcttggcaa tgctgcagaa tgagttggag gggctggggg
     3601 acatcttcca ccccatgaat gcggaggagc aagctcagct ggctgcctcc cagcctgggc
     3661 caaaggtgct gtcggcggaa caggggagct acttcgttcg tttaggtgac ctgggtccca
     3721 gcttccgcca gcgggcattt gaacacgcgg tgagccacct gcagcacggc cagttccaag
     3781 ccagggacac tctggcccag ctccaggact gcttcaggct gattgaaaag gcccagcagg
     3841 ctccagaagg gcagccacgt ctggaccagg gctcaggtgc cagtgcggag gacgctgctg
     3901 tccaggagga gcgggatgcc ggggttctgt ccagggtctg cggccttctc cggcagctgc
     3961 acacggccta cagtggcctg gtctccagcc tccagggcct gcccgccgag ctccagcagc
     4021 cagtggggcg ggcgcggcac agcctctgtg agctctatgg catcgtggcc tcagctggct
     4081 ctgtagagga gctgcccgca gagcggctgg tgcagagccg cgagggtgtg caccaggctt
     4141 ggcaggggtt agagcagctg ctggagggcc tacagcacaa tcccccgctc agctggctgg
     4201 tagggccctt cgccttgccc gctggcgggc agtagctgta ggagcctgca ggcccggcgc
     4261 ggggtcgccc tgctctgtcc agggaggagc tgcctcagaa ctttctcccc gcccccaaac
     4321 ctggatcggt tccctaaagc cctagacctt tggggctgca gctggctgag cgccgagggg
     4381 ctgcggaggc agtgaccttc ttaactgagc caccccacgc cctgctccgg gcctgcctgc
     4441 atctcccacc tcctccccag cgctgcctgc ccctctcgga gcctggggtc actcagacca
     4501 ccagccaaga gccttccctt gaagtcccca agcaagcact gcaattagga aagagaaaaa
     4561 gcagcgtgcc cagcctggaa gggcatctgt ttgccccgct agcaaccctt ttatatctag
     4621 cagggctctt ccagtcctgc agcacgggcc cccagctatc agcggtgcag gcagtgctgt
     4681 ggcatcccag gctccgggca gctccgttct catgctgaaa gtgggtctcc ggccttagca
     4741 cacacacctt gagggtctta agaaccacat tccctcatag tagaaagtac tagaaaaagc
     4801 gacactgcca tcatcatccc aaggcaggct gctactgcct ttgctgaccc ccggggtggc
     4861 ctcacggtgg ggacaaagct gccaggagcc acagcagcca cagctggggc tttgcaccag
     4921 cctggcttga gactgagcag tttgcagggg gtggggggtg caaaaaacaa gcaaacaggc
     4981 tgctgctgcc tccagctgcc caccacaggc ctgccccagg cacctggggc tctgaggccc
     5041 ctggggaggc tgggcccagc agctgcccct ggagaacaca gacaaaggac ttccccgcag
     5101 ggaactgtgc cctatggagg gatcagacag ggctgggaac agccacagag gctgcgtgcc
     5161 tatggcacag cccttcctcc gccgcacact ccccctgggt cctcaggccc acccaagcgc
     5221 cgggctgcag aggaagcggg gctggggagg ctgcaggcat cagagacact ggtggtggcg
     5281 gacccggccg ccgggccccg tgctctcagg ctagcccagg tcgtggaggc tggcaggctc
     5341 aggtcgggtg tgagacgtgc cgtggctgcg ctcagtccag cggggaggag ccgttcagcc
     5401 cggcctcccc aggaagccat atccccactc acccggtaag agaaccttgt cgtccccttt
     5461 ccatgctctc ctaggacacg agcccaggaa ccccagaccc agggggagga agggtggagg
     5521 ggccccaggg gtcaccgtgt gcaccagggg ccgtgagggg ccggggcatt cagctcagct
     5581 ctgaaccggg gaagctggca cggcaaggac tgcctcaggt gacgggccgt gagaggggac
     5641 gggtcaggag ccttcccaag ccttctcctc agcccgacac ccatggccat cggaggctag
     5701 gatgccagac acagccattt gcagaaatca ggcacagtga ctgcagctca cgtccagcca
     5761 accaagcatg gggccgcagc tcaggaagtc ccttcccgcc acaccacagc ctaattctta
     5821 ctgggacgga ggcaactcgg ctacgctggg caggacgaca aacacgagac gccactgtgg
     5881 aatgagcaac ttcggagcac ggggtgactt gcttgggacc gtgcccacgt gacagcccct
     5941 tatgcagagg aggaaagaga agccccgagt gggaggggaa cctgtccaaa gtcacacggt
     6001 gtgtgggtga cacagctggg gtgagtcgag gctggcccct gaggcccatg ctccctgaac
     6061 gctggagacc actgtcggct agcagcggct ctcagggaag gcctggtctc caccctccca
     6121 gcctagcctc gcggaccctc gtcctcccca catcggacct gctcacctgc ctggaccctg
     6181 ggctgccaga tgcaggaagc atcaaacccc ccagcctcgt gggtgcgggg cagggcgcag
     6241 gcagcacagc ttagatgccc tggtttgtcc ctcttgtctc ctgggaagag cttgctcccg
     6301 cccagctctc ctgccactgg cctttcaggg ttgggctggg cccagagtgc cttttagtcg
     6361 cttctcacgg tggcctgatg gctcaaccca gtcccaaacg ggcccagtga cactgccgcc
     6421 tgcgccccag ctcaggcccc cactgcacca gcaatgctag aaaaccaagc caataaaagt
     6481 gatttctttt ttcattaaaa aa
//
