LOCUS       NM_025201               3689 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens pleckstrin homology domain containing O2 (PLEKHO2),
            transcript variant 1, mRNA.
ACCESSION   NM_025201
VERSION     NM_025201.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Liu,S., An,G., Cao,Q., Li,T., Jia,X. and Lei,L.
  TITLE     The miR-106b/NR2F2-AS1/PLEKHO2 Axis Regulates Migration and
            Invasion of Colorectal Cancer through the MAPK Pathway
  JOURNAL   Int J Mol Sci 22 (11), 5877 (2021)
   PUBMED   34070923
  REMARK    GeneRIF: The miR-106b/NR2F2-AS1/PLEKHO2 Axis Regulates Migration
            and Invasion of Colorectal Cancer through the MAPK Pathway.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 3689)
  AUTHORS   Zhang,P., Zhou,C., Lu,C., Li,W., Li,W., Jing,B., Chen,W., Zha,Y.,
            Zhang,P., Bai,C., Liu,H. and Zhang,L.
  TITLE     PLEKHO2 is essential for M-CSF-dependent macrophage survival
  JOURNAL   Cell Signal 37, 115-122 (2017)
   PUBMED   28627369
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK311036.1, BC008744.2 and AC069368.14.
            
            On Nov 23, 2018 this sequence version replaced NM_025201.4.
            
            Transcript Variant: This variant (1) is the longer transcript and
            encodes the longer protein isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.203100.1,
                                           SRR1660807.69532.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000323544.5/ ENSP00000326706.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-585               AK311036.1         33-617
            586-2945            BC008744.2         429-2788
            2946-3664           AC069368.14        102550-103268       c
            3665-3689           BC008744.2         3508-3532
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q22.31"
     gene            1..3689
                     /gene="PLEKHO2"
                     /gene_synonym="PLEKHQ1; PP1628; pp9099"
                     /note="pleckstrin homology domain containing O2"
                     /db_xref="GeneID:80301"
                     /db_xref="HGNC:HGNC:30026"
     exon            1..114
                     /gene="PLEKHO2"
                     /gene_synonym="PLEKHQ1; PP1628; pp9099"
                     /inference="alignment:Splign:2.1.0"
     CDS             103..1575
                     /gene="PLEKHO2"
                     /gene_synonym="PLEKHQ1; PP1628; pp9099"
                     /note="isoform 1 is encoded by transcript variant 1;
                     pleckstrin homology domain-containing family O member 2;
                     PH domain-containing protein; PH domain-containing family
                     O member 2; PH domain-containing family Q member 1;
                     pleckstrin homology domain-containing family Q member 1"
                     /codon_start=1
                     /product="pleckstrin homology domain-containing family O
                     member 2 isoform 1"
                     /protein_id="NP_079477.2"
                     /db_xref="CCDS:CCDS10196.1"
                     /db_xref="GeneID:80301"
                     /db_xref="HGNC:HGNC:30026"
                     /translation="MEEEGVKEAGEKPRGAQMVDKAGWIKKSSGGLLGFWKDRYLLLC
                     QAQLLVYENEDDQKCVETVELGSYEKCQDLRALLKRKHRFILLRSPGNKVSDIKFQAP
                     TGEEKESWIKALNEGINRGKNKAFDEVKVDKSCALEHVTRDRVRGGQRRRPPTRVHLK
                     EVASAASDGLLRLDLDVPDSGPPVFAPSNHVSEAQPRETPRPLMPPTKPFLAPETTSP
                     GDRVETPVGERAPTPVSASSEVSPESQEDSETPAEEDSGSEQPPNSVLPDKLKVSWEN
                     PSPQEAPAAESAEPSQAPCSETSEAAPREGGKPPTPPPKILSEKLKASMGEMQASGPP
                     APGTVQVSVNGMDDSPEPAKPSQAEGTPGTPPKDATTSTALPPWDLPPQFHPRCSSLG
                     DLLGEGPRHPLQPRERLYRAQLEVKVASEQTEKLLNKVLGSEPAPVSAETLLSQAVEQ
                     LRQATQVLQEMRDLGELSQEAPGLREKRKELVTLYRRSAP"
     exon            115..264
                     /gene="PLEKHO2"
                     /gene_synonym="PLEKHQ1; PP1628; pp9099"
                     /inference="alignment:Splign:2.1.0"
     exon            265..381
                     /gene="PLEKHO2"
                     /gene_synonym="PLEKHQ1; PP1628; pp9099"
                     /inference="alignment:Splign:2.1.0"
     exon            382..486
                     /gene="PLEKHO2"
                     /gene_synonym="PLEKHQ1; PP1628; pp9099"
                     /inference="alignment:Splign:2.1.0"
     exon            487..585
                     /gene="PLEKHO2"
                     /gene_synonym="PLEKHQ1; PP1628; pp9099"
                     /inference="alignment:Splign:2.1.0"
     exon            586..3689
                     /gene="PLEKHO2"
                     /gene_synonym="PLEKHQ1; PP1628; pp9099"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3669..3674
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLEKHO2"
                     /gene_synonym="PLEKHQ1; PP1628; pp9099"
                     /note="hexamer: AATAAA"
     polyA_site      3689
                     /gene="PLEKHO2"
                     /gene_synonym="PLEKHQ1; PP1628; pp9099"
                     /note="major polyA site"
ORIGIN      
        1 agaacgcgga gagtcgccgc ctggccgggc gtagacgcgg tggcagagcc cgcgcggcgc
       61 tggaagcgag tggcggagcg gcgggacctc ggcggactcg ccatggagga ggagggtgtg
      121 aaggaagccg gtgagaagcc tcggggagca cagatggtgg acaaggctgg ctggatcaag
      181 aagagcagtg ggggcctcct gggtttctgg aaagaccgat atctgctcct ctgccaggcc
      241 cagctgctgg tctatgagaa tgaggatgat cagaagtgtg tggagactgt ggagctgggc
      301 agctatgaga agtgccagga ccttcgtgcc ctcctcaagc gaaaacaccg ctttatcctg
      361 ctgcgatccc cagggaacaa ggtcagcgac atcaaattcc aggcacccac cggggaggag
      421 aaggaatcct ggatcaaagc cctcaatgaa gggattaacc gaggcaaaaa caaggctttc
      481 gatgaggtaa aggtggacaa gagctgcgcc ctggagcatg tgacacggga ccgggtgcga
      541 gggggccagc gacgccggcc accaacgaga gtccacctga aggaggtggc cagtgcagct
      601 tctgacggtc ttctgcgcct ggatcttgat gttccggaca gtgggccacc agtgtttgcc
      661 cccagcaatc atgtcagtga agcccaacct cgggagacac cccggcccct catgcctcct
      721 accaagcctt tcctagcacc tgagaccacc agccctggtg acagggtgga gacccctgtg
      781 ggggagagag ccccaacccc tgtctcagca agctctgagg tctcccctga gagccaagag
      841 gactcagaga ccccagcaga ggaggacagt ggctctgagc agcctcccaa cagcgtcctg
      901 cctgacaaac tgaaggtgag ctgggagaac cccagccccc aggaggcccc tgctgcagag
      961 agtgcagaac cgtcccaggc accctgttct gagacttctg aggctgcccc cagggagggt
     1021 gggaagcccc ctacaccccc acccaagatc ttatcagaga aactgaaagc ctccatgggt
     1081 gagatgcagg cttctgggcc acctgctcca ggcacagtgc aggtctcagt gaatggcatg
     1141 gatgacagtc ctgagcctgc caagccctct caggctgagg gcaccccagg aactcctcca
     1201 aaggatgcaa caacatccac agcactgccc ccctgggacc tgccacctca gttccatccc
     1261 cgctgctcct cccttgggga cttgcttggg gaaggcccgc ggcatccctt gcagcccagg
     1321 gaacggctat atcgggccca gctggaggtg aaggtggcct cggaacagac ggagaaactg
     1381 ttgaacaagg tgctgggcag tgagccggcc cctgttagtg ccgaaacatt gctcagccag
     1441 gctgtggagc agctgaggca ggccacccag gtcctgcagg aaatgagaga tttgggagag
     1501 ctgagccagg aagcacctgg gctaagggag aagcggaagg agctggtgac cctctacagg
     1561 agaagtgcac cctagggcct tctgggccag aggcaccatc ccttctggcc atccatcaag
     1621 tccatcaagg cccagccctg ctgagaaatg tgcttctgct tctacagcaa tggctgcagg
     1681 agggccattg ggcatgtcag ggtttggcca tgacccgaag agactcctgg cgtccttcct
     1741 actctgctct ggccagtggt gccaggtgcc acccagggct actgcctggc tatctggcct
     1801 ggcctctggg ctggggctgg ggctgggagc acacacgctg ggacctatgt gtttgtgtgg
     1861 tcgttccaaa ctgccccagg gctttggggg cggcacttgg ggtttctggg aatgacatca
     1921 tctctgttcc ccatccccag tagtttacat tcctgacttc tgaatacagc acagctgagc
     1981 cccctgcagc tcccatctcc agctattcct aggcaaagag cctcatggct aaggcagcct
     2041 caaagccagc ccctcctccc acctattctg agtagctgca gaggccttgg gtccaggctc
     2101 taggttcatc cctcagttgg ggggaacgta ggacccagct ggagcctctt gagggagatg
     2161 agaggcctct ttgtgaggag gacattagct gtgtggcctc tctctctttg gccctgtttc
     2221 cttttttgca aaacaaggac attttctgca gccccttcct ctcagtgagc tatgattgga
     2281 gggcttaggt ctggaggatt caagagtgga agaggaattt aaggggtccc ctagtctagt
     2341 ctctgcccct ggatagtgtc cagccttgta tatttctgaa gaggtggatc ccagagtggc
     2401 tctgatgtcc acattagaaa aacttacttg taatgatcat gtcagccttc agaagagaat
     2461 ccccaccaac ttctgtgcct cctcagatgg ggatttatct ggatctctgt ggttccttct
     2521 cagccgaaac aggtccagta tcccagtcat ttcttcaaat gctgataggg gtatgttgga
     2581 atccgaagcc acttccccgc cttcaagccc cagatgggct gctctcctgt aactttctag
     2641 gagaagagac attttcttct ttccctttcc tggtccatcc ctgcaccctg gtcctctccc
     2701 agcctctccc ccacattgtc cctgactcta ggggcacatc cagtctccat cgtgctgcag
     2761 cagctggact gagggcagag cctgtaggtg cagaggccct ggctcccgag gtccagccac
     2821 tctccctggg gcctctgggg tgagagcagc ttccgatagg acctgcccag atttctgcat
     2881 gtgcactttt gtttactgaa agagagaaag gggggggtca cagcaacatg ccctggcctt
     2941 tctgctctgt tccccaaccc cactgaggcc tgctgcacag gtcaatgcct tcgttatcgt
     3001 tattgtactg tcactttgtt cttgaggtag tagtcaagga tcaggagggg cagatgtctt
     3061 ctctgggctg cgtggggccg gagcagaggt gagcagcaat gcactggttc gggagccccc
     3121 atcagcctcc ttgtgcaaac tgggccccca tgccacagtc tggctttccc tccatctgcc
     3181 ccaggacaag agcaagaagg acatcagttg cccagtcatg tgatcccctg ccatcttgcc
     3241 ttaggaacag ccttccccca ccagcagcca tggctggctg gggctttagc caagccacct
     3301 actgccagga attggagcct cagttccctc ctgtgtcaag tagctaactg cagcagctgg
     3361 actgagggca gagtctgtgg gtgcagagac cctgcatgta ggtcacaggt tgaggcccag
     3421 ccactctccc tggggcctgg tgggtaggca agtagctctg gggccacctc aagtgaccaa
     3481 atgctattaa tttccatcct ttagcaggct gggccctagg caggaagctg gcttctggga
     3541 gaggagtgag aacgtgcagg gcctgcctag cttgcgtgct tgaggaaggt ggcattccgt
     3601 gcttgcctcc ttgaggaggg tggcattctg tgtcttctgc ttatgaagcg cctttcttaa
     3661 agtttggcaa taaatccatt tttatggaa
//
