LOCUS       NM_014798               5240 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens pleckstrin homology and RUN domain containing M1
            (PLEKHM1), transcript variant 1, mRNA.
ACCESSION   NM_014798 XM_038655
VERSION     NM_014798.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Ding,S.A., Liu,H., Zheng,R., Ge,Y., Fu,Z., Mei,J. and Tang,M.
  TITLE     Downregulation of MYBL1 in endothelial cells contributes to
            atherosclerosis by repressing PLEKHM1-inducing autophagy
  JOURNAL   Cell Biol Toxicol 40 (1), 40 (2024)
   PUBMED   38797732
  REMARK    GeneRIF: Downregulation of MYBL1 in endothelial cells contributes
            to atherosclerosis by repressing PLEKHM1-inducing autophagy.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Huybrechts,Y. and Van Hul,W.
  TITLE     Osteopetrosis associated with PLEKHM1 and SNX10 genes, both
            involved in osteoclast vesicular trafficking
  JOURNAL   Bone 164, 116520 (2022)
   PUBMED   35981699
  REMARK    GeneRIF: Osteopetrosis associated with PLEKHM1 and SNX10 genes,
            both involved in osteoclast vesicular trafficking.
REFERENCE   3  (bases 1 to 5240)
  AUTHORS   Gubas,A., Karantanou,C., Popovic,D., Tascher,G., Hoffmann,M.E.,
            Platzek,A., Dawe,N., Dikic,I., Krause,D.S. and McEwan,D.G.
  TITLE     The endolysosomal adaptor PLEKHM1 is a direct target for both mTOR
            and MAPK pathways
  JOURNAL   FEBS Lett 595 (7), 864-880 (2021)
   PUBMED   33452816
  REMARK    GeneRIF: The endolysosomal adaptor PLEKHM1 is a direct target for
            both mTOR and MAPK pathways.
REFERENCE   4  (bases 1 to 5240)
  AUTHORS   Marwaha,R., Arya,S.B., Jagga,D., Kaur,H., Tuli,A. and Sharma,M.
  TITLE     The Rab7 effector PLEKHM1 binds Arl8b to promote cargo traffic to
            lysosomes
  JOURNAL   J Cell Biol 216 (4), 1051-1070 (2017)
   PUBMED   28325809
REFERENCE   5  (bases 1 to 5240)
  AUTHORS   Fujiwara,T., Ye,S., Castro-Gomes,T., Winchell,C.G., Andrews,N.W.,
            Voth,D.E., Varughese,K.I., Mackintosh,S.G., Feng,Y., Pavlos,N.,
            Nakamura,T., Manolagas,S.C. and Zhao,H.
  TITLE     PLEKHM1/DEF8/RAB7 complex regulates lysosome positioning and bone
            homeostasis
  JOURNAL   JCI Insight 1 (17), e86330 (2016)
   PUBMED   27777970
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5240)
  AUTHORS   Tabata,K., Matsunaga,K., Sakane,A., Sasaki,T., Noda,T. and
            Yoshimori,T.
  TITLE     Rubicon and PLEKHM1 negatively regulate the endocytic/autophagic
            pathway via a novel Rab7-binding domain
  JOURNAL   Mol Biol Cell 21 (23), 4162-4172 (2010)
   PUBMED   20943950
  REMARK    GeneRIF: Rubicon and PLEKHM1 specifically and directly interact
            with Rab7 via their RH domain; this interaction is critical for
            their function; show Rubicon but not PLEKHM1 uniquely regulates
            membrane trafficking via simultaneously binding both Rab7 and
            PI3-kinase
REFERENCE   7  (bases 1 to 5240)
  AUTHORS   Edwards,T.L., Scott,W.K., Almonte,C., Burt,A., Powell,E.H.,
            Beecham,G.W., Wang,L., Zuchner,S., Konidari,I., Wang,G., Singer,C.,
            Nahab,F., Scott,B., Stajich,J.M., Pericak-Vance,M., Haines,J.,
            Vance,J.M. and Martin,E.R.
  TITLE     Genome-wide association study confirms SNPs in SNCA and the MAPT
            region as common risk factors for Parkinson disease
  JOURNAL   Ann Hum Genet 74 (2), 97-109 (2010)
   PUBMED   20070850
REFERENCE   8  (bases 1 to 5240)
  AUTHORS   Del Fattore,A., Fornari,R., Van Wesenbeeck,L., de Freitas,F.,
            Timmermans,J.P., Peruzzi,B., Cappariello,A., Rucci,N., Spera,G.,
            Helfrich,M.H., Van Hul,W., Migliaccio,S. and Teti,A.
  TITLE     A new heterozygous mutation (R714C) of the osteopetrosis gene,
            pleckstrin homolog domain containing family M (with run domain)
            member 1 (PLEKHM1), impairs vesicular acidification and increases
            TRACP secretion in osteoclasts
  JOURNAL   J Bone Miner Res 23 (3), 380-391 (2008)
   PUBMED   17997709
  REMARK    GeneRIF: heterozygous mutation (R714C) of the osteopetrosis gene,
            pleckstrin homolog domain containing family M (with run domain)
            member 1 (PLEKHM1), impairs vesicular acidification and increases
            TRACP secretion in osteoclasts
REFERENCE   9  (bases 1 to 5240)
  AUTHORS   Van Wesenbeeck,L., Odgren,P.R., Coxon,F.P., Frattini,A., Moens,P.,
            Perdu,B., MacKay,C.A., Van Hul,E., Timmermans,J.P.,
            Vanhoenacker,F., Jacobs,R., Peruzzi,B., Teti,A., Helfrich,M.H.,
            Rogers,M.J., Villa,A. and Van Hul,W.
  TITLE     Involvement of PLEKHM1 in osteoclastic vesicular transport and
            osteopetrosis in incisors absent rats and humans
  JOURNAL   J Clin Invest 117 (4), 919-930 (2007)
   PUBMED   17404618
  REMARK    GeneRIF: PLEKHM1 is a novel gene implicated in the development of
            osteopetrosis, with a putative critical function in vesicular
            transport in the osteoclast.
REFERENCE   10 (bases 1 to 5240)
  AUTHORS   Hartel-Schenk,S., Gratchev,A., Hanski,M.L., Ogorek,D.,
            Trendelenburg,G., Hummel,M., Hopfner,M., Scherubl,H., Zeitz,M. and
            Hanski,C.
  TITLE     Novel adapter protein AP162 connects a sialyl-Le(x)-positive mucin
            with an apoptotic signal transduction pathway
  JOURNAL   Glycoconj J 18 (11-12), 915-923 (2001)
   PUBMED   12820725
  REMARK    GeneRIF: These data indicate that B2 is a novel, ubiquitously
            expressed protein with a putative adapter function. The protein has
            been named AP162.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB064851.1 and BC064361.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_014798.2.
            
            Summary: The protein encoded by this gene is essential for bone
            resorption, and may play a critical role in vesicular transport in
            the osteoclast. Mutations in this gene are associated with
            autosomal recessive osteopetrosis type 6 (OPTB6). Alternatively
            spliced transcript variants have been found for this gene.
            [provided by RefSeq, Sep 2009].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC064361.1, AB002354.2 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000430334.8/ ENSP00000389913.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-442               DB064851.1         94-535
            443-5240            BC064361.1         466-5263
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q21.31"
     gene            1..5240
                     /gene="PLEKHM1"
                     /gene_synonym="AP162; B2; OPTA3; OPTB6"
                     /note="pleckstrin homology and RUN domain containing M1"
                     /db_xref="GeneID:9842"
                     /db_xref="HGNC:HGNC:29017"
                     /db_xref="MIM:611466"
     exon            1..70
                     /gene="PLEKHM1"
                     /gene_synonym="AP162; B2; OPTA3; OPTB6"
                     /inference="alignment:Splign:2.1.0"
     exon            71..159
                     /gene="PLEKHM1"
                     /gene_synonym="AP162; B2; OPTA3; OPTB6"
                     /inference="alignment:Splign:2.1.0"
     CDS             112..3282
                     /gene="PLEKHM1"
                     /gene_synonym="AP162; B2; OPTA3; OPTB6"
                     /note="isoform 1 is encoded by transcript variant 1;
                     pleckstrin homology domain-containing family M member 1;
                     162 kDa adapter protein; pleckstrin homology domain
                     containing, family M (with RUN domain) member 1; PH
                     domain-containing family M member 1"
                     /codon_start=1
                     /product="pleckstrin homology domain-containing family M
                     member 1 isoform 1"
                     /protein_id="NP_055613.1"
                     /db_xref="CCDS:CCDS32671.1"
                     /db_xref="GeneID:9842"
                     /db_xref="HGNC:HGNC:29017"
                     /db_xref="MIM:611466"
                     /translation="MLSVVENGLDPQAAIPVIKKKLVGSVKALQKQYVSLDTVVTSED
                     GDANTMCSALEAVFIHGLHAKHIRAEAGGKRKKSAHQKPLPQPVFWPLLKAVTHKHII
                     SELEHLTFVNTDVGRCRAWLRLALNDGLMECYLKLLLQEQARLHEYYQPTALLRDAEE
                     GEFLLSFLQGLTSLSFELSYKSAILNEWTLTPLALSGLCPLSELDPLSTSGAELQRKE
                     SLDSISHSSGSEDIEVHHSGHKIRRNQKLTASSLSLDTASSSQLSCSLNSDSCLLQEN
                     GSKSPDHCEEPMSCDSDLGTANAEDSDRSLQEVLLEFSKAQVNSVPTNGLSQETEIPT
                     PQASLSLHGLNTSTYLHCEAPAEPLPAQAASGTQDGVHVQEPRPQAPSPLDLQQPVES
                     TSGQQPSSTVSETAREVGQGNGLQKAQAHDGAGLKLVVSSPTSPKNKSWISEDDFYRP
                     SREQPLESASDHPIASYRGTPGSRPGLHRHFSQEPRKNCSLGALDQACVPSPGRRQAQ
                     AAPSQGHKSFRVVHRRQMGLSNPFRGLMKLGTVERRGAMGIWKELFCELSPLEFRLYL
                     SNEEHTCVENCSLLRCESVGPAHSDGRFELVFSGKKLALRASSQDEAEDWLDRVREAL
                     QKVRPQQEDEWVNVQYPDQPEEPPEAPQGCLSPSDLLSEPAALQGTQFDWSSAQVPEP
                     DAIKESLLYLYMDRTWMPYIFSLSLEALKCFRIRNNEKMLSDSHGVETIRDILPDTSL
                     GGPSFFKIITAKAVLKLQAGNAEEAALWRDLVRKVLASYLETAEEAVTLGGSLDENCQ
                     EVLKFATRENGFLLQYLVAIPMEKGLDSQGCFCAGCSRQIGFSFVRPKLCAFSGLYYC
                     DICHQDDASVIPARIIHNWDLTKRPICRQALKFLTQIRAQPLINLQMVNASLYEHVER
                     MHLIGRRREQLKLLGDYLGLCRSGALKELSKRLNHRNYLLESPHRFSVADLQQIADGV
                     YEGFLKALIEFASQHVYHCDLCTQRGFICQICQHHDIIFPFEFDTTVRCAECKTVFHQ
                     SCQAVVKKGCPRCARRRKYQEQNIFA"
     exon            160..407
                     /gene="PLEKHM1"
                     /gene_synonym="AP162; B2; OPTA3; OPTB6"
                     /inference="alignment:Splign:2.1.0"
     exon            408..1034
                     /gene="PLEKHM1"
                     /gene_synonym="AP162; B2; OPTA3; OPTB6"
                     /inference="alignment:Splign:2.1.0"
     exon            1035..1419
                     /gene="PLEKHM1"
                     /gene_synonym="AP162; B2; OPTA3; OPTB6"
                     /inference="alignment:Splign:2.1.0"
     exon            1420..1690
                     /gene="PLEKHM1"
                     /gene_synonym="AP162; B2; OPTA3; OPTB6"
                     /inference="alignment:Splign:2.1.0"
     exon            1691..2608
                     /gene="PLEKHM1"
                     /gene_synonym="AP162; B2; OPTA3; OPTB6"
                     /inference="alignment:Splign:2.1.0"
     exon            2609..2754
                     /gene="PLEKHM1"
                     /gene_synonym="AP162; B2; OPTA3; OPTB6"
                     /inference="alignment:Splign:2.1.0"
     exon            2755..2948
                     /gene="PLEKHM1"
                     /gene_synonym="AP162; B2; OPTA3; OPTB6"
                     /inference="alignment:Splign:2.1.0"
     exon            2949..3012
                     /gene="PLEKHM1"
                     /gene_synonym="AP162; B2; OPTA3; OPTB6"
                     /inference="alignment:Splign:2.1.0"
     exon            3013..3170
                     /gene="PLEKHM1"
                     /gene_synonym="AP162; B2; OPTA3; OPTB6"
                     /inference="alignment:Splign:2.1.0"
     exon            3171..5240
                     /gene="PLEKHM1"
                     /gene_synonym="AP162; B2; OPTA3; OPTB6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5216..5221
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLEKHM1"
                     /gene_synonym="AP162; B2; OPTA3; OPTB6"
                     /note="hexamer: AGTAAA"
     polyA_site      5240
                     /gene="PLEKHM1"
                     /gene_synonym="AP162; B2; OPTA3; OPTB6"
                     /note="major polyA site"
ORIGIN      
        1 atcgtcggct cggaggctga ggggctgccg cggccgggag cgcccctcgc ctcgctcctc
       61 gctccgcttg gtgtcatgtg attctctgag ggagcagctg cgtgagtgga gatgctttca
      121 gtggtggaga atggactgga cccccaggct gccatcccgg tcatcaagaa gaagctggtg
      181 ggatccgtga aggccttgca gaagcagtac gtgtccctgg acacggtggt cactagtgaa
      241 gacggagatg ccaacaccat gtgcagcgcc ctggaggccg tatttatcca tggcctgcac
      301 gccaagcaca tccgagctga ggccggagga aaaaggaaga aaagtgccca ccagaagcct
      361 ctgccccagc ctgtcttctg gcccctcctg aaagctgtca cccacaaaca catcatctca
      421 gagttggagc acctgacgtt tgtcaacacg gatgtgggcc gctgccgggc atggctgcgg
      481 ctggccctga acgatggcct gatggagtgc tacctgaagc tgctgctgca ggagcaggcc
      541 cgcttgcatg agtactacca gcccaccgcc ctgctccggg atgctgagga gggcgagttc
      601 ctccttagct tcctgcaggg cctcacgtcc ttgtccttcg aactctccta caagtctgcc
      661 atcttaaatg agtggacgct caccccattg gccctgtctg ggctttgccc gctttctgag
      721 ctggaccctc tctctacctc tggtgcagaa ctacagcgga aggaatctct ggattccatt
      781 tcccattctt caggctctga agacatcgaa gtccatcact cgggccataa gatacggagg
      841 aaccagaagc tgactgcctc ctccctcagc ctggacacgg ccagttcatc ccagctgtcc
      901 tgcagcctaa actctgatag ctgcttactc caagagaatg gctccaagag tccagaccat
      961 tgcgaggagc ccatgtcctg tgactcagac ctgggcacag caaatgctga ggactcagac
     1021 cggtctctgc aagaggtatt gttggaattc agcaaagccc aggtaaactc tgtgccaacc
     1081 aacggactga gccaagaaac agagatcccc acaccacagg cctcgctctc cctccatggc
     1141 ctcaacacca gcacatacct gcactgtgag gcacctgcag agccccttcc tgcccaggca
     1201 gcctctggaa ctcaagatgg tgtccacgtg caggagccgc gtccccaggc gcccagcccc
     1261 ctggacttac agcagcctgt agagagcacc tcaggccagc agccttctag tactgtcagc
     1321 gagacagcca gagaagtggg ccaagggaat ggcctgcaga aggcccaggc tcatgacgga
     1381 gctggtctga agctggtagt ttcctcaccc accagtccga aaaacaagag ctggatctca
     1441 gaggatgact tctaccggcc ttcccgggag caacccctgg agagtgcttc agaccaccca
     1501 atagcttctt acagggggac tccagggtca aggcctggtc tccacaggca tttttctcaa
     1561 gaaccaagaa aaaactgctc cctgggggcg ttagaccaag cgtgtgtacc ttccccagga
     1621 agaaggcaag cccaggcagc cccatcccag gggcataaga gcttccgggt ggtacaccgg
     1681 agacagatgg gactgtccaa cccattccgg ggtctcatga agctgggcac cgtggagcgg
     1741 cggggggcaa tgggcatctg gaaggagctc ttctgcgagc tctccccgct ggagttccgc
     1801 ctctacctga gcaacgagga gcacacctgt gtggagaact gctcgctgct tcgctgtgag
     1861 tctgtggggc cagcccatag tgatgggcgc tttgagctgg tcttctctgg caagaagctg
     1921 gccctgcgcg cctcctccca ggacgaagct gaggactggc tggaccgggt gcgggaggcc
     1981 ctgcagaagg tccggcctca gcaggaggat gagtgggtga acgtgcagta cccagaccag
     2041 cctgaggaac cccccgaggc gccccagggc tgcctctctc cctcagacct gctctcggag
     2101 cccgcggccc tccagggcac acagtttgac tggtcgtccg cccaggttcc agagccagat
     2161 gccatcaagg agtccctgct gtacttgtac atggacagga cctggatgcc ctatatattt
     2221 tctctgtcct tggaggctct gaaatgtttc cgcatcagga acaatgagaa gatgctgagt
     2281 gacagccacg gcgtggagac catccgggac atcctgccag acaccagcct tgggggccca
     2341 tccttcttca aaatcatcac ggccaaggct gtcctgaagc tgcaggccgg aaacgccgag
     2401 gaagccgccc tgtggaggga tctggtccgc aaagtcctgg catcctactt ggagacagcc
     2461 gaggaggcgg tgaccctggg cgggagcctg gatgaaaact gtcaggaggt gctgaaattt
     2521 gccacccggg agaatggctt cctgctgcag tacctggtgg ctatccccat ggagaaaggc
     2581 cttgactccc aaggctgctt ctgcgcaggc tgctcccggc agatcggctt ctcctttgta
     2641 cgacccaagc tctgtgcctt ctctggcctc tattactgtg acatctgcca ccaagacgat
     2701 gcctcagtga ttccggccag gatcatccac aactgggacc tcaccaagcg cccgatctgc
     2761 aggcaggccc tgaagtttct gacacagatc cgggcccagc ccctcatcaa cctgcagatg
     2821 gtgaacgcgt ctctgtacga gcatgtggag cggatgcacc tcattgggag gagacgggag
     2881 cagctgaagc tcctggggga ttacctgggc ctgtgccgga gtggcgccct gaaggagctc
     2941 agcaagaggc tcaaccacag gaattatctc ttggaatctc cgcataggtt cagtgttgct
     3001 gacctccaac agatcgcaga cggggtgtat gaaggattcc tcaaggccct gattgaattt
     3061 gcctcccagc atgtctacca ctgcgacctg tgcacccagc gcggcttcat ctgccagatc
     3121 tgccagcacc acgacatcat cttccccttt gagtttgaca ccacagtcag gtgtgccgag
     3181 tgcaagaccg tcttccacca gagctgccag gctgtggtga agaagggctg cccccgctgt
     3241 gcccgccggc gcaagtacca ggaacagaac attttcgcct gatgcccatc tgctgacccc
     3301 gctctgaaag ccgggggtga gtgtggctca gccatcccgg ctgggtttgc catcagccca
     3361 ggatactcac cgtgtcacag ctgtgtcccc ttgtcaggaa gaccctcaga tgtggccaga
     3421 gcaccggcct cccagagaaa gcccaggaag tccccgtggt gtcccctggc cctcccccca
     3481 cccctctgct gcaggaggcg tggccaccac cagatgctcc ctgtctgggg caggcagggc
     3541 cgtttactca ccaggccctg gctcaccagc gtcccggcgc ctccgttagg gctgcccaaa
     3601 cactgtggaa aggggacttg gggagcattt tcaattccac attcctgatt aaaaggtcta
     3661 gttttttaag gtgggttttt cccattcata tttcaacaga aagtattttt ttattcttga
     3721 ccctacgtga gtcacccatg aaatccagac attctcaccc ccagcggatg cacaggagcc
     3781 actcaggcct ggcttagagg tagcgggtgg ctggtgggca ggccactgct gggacagggt
     3841 ctatgggccc acccagtgcc tttccttggc cctcactgcc agtgccaaac cgcccctcag
     3901 cagggcaggg ccagtcctgg cgaccccggt tcctctgtgt cctctttact ccccacccca
     3961 gtgcgtctcg cctgctgagc ccaccgcttt ctcagcgtca gagcccctag ctcttctcta
     4021 gcctggggcc ttggccccat taggagaagg agaaatgact ggaaaccagc actgtcagca
     4081 ctttgagccc tcctcggttt atggagggtc gccgtcttgc ccacccccga tccccgtgcg
     4141 ctcctcaccc ctctacagac aggggcgctg ctgcagatgc tccaggccca ccttggtggg
     4201 tgcccggggt cacactctct gcctgactga cccctgccag ggcacagata ggcaggaccc
     4261 cagagagggc aggcagccac gccctgcact gtggagaagt gacctggtcc taagctgtgc
     4321 agggcgtgcg ggggaactgc cctgtggatg cagccaccac ctcacaaagc catgaattcc
     4381 caaagcatca ctgaacttgc ccagccggga cactggagac tgaagaagtt ccccggagca
     4441 ggactgcctt tgaaggggcc ttggggctgg ggtgggagtc tggaattgtt ttcaaagtga
     4501 tggtcagacc ctccaggctt cggggaaatg tgggtttgct ggtctctctg tcgccttccc
     4561 ccacctctcc tggacctagc aggccctcag tcctagctgt cttacctgcg ctcagcttgg
     4621 agtcaggcct caggtggaga gccgcatgtc actgtcagag atgagcccac ccccaccctt
     4681 ccctgggggc gattagctgc tgagtcaccc acccagcctt ggagctggat gcctgtgcag
     4741 cagaacagag gcaacggtgg caggcaggtg gaggctgcgc acagcttctc ccggtcatgg
     4801 cacacacctg tgcccagacg ccaatgcctc ctaagccagg agctgggtta tgcaccgcca
     4861 tggcccacat taacgctcca ccacaagccc agcctagtca gagccctcat tgccccatcc
     4921 ctgagtgggc aggagcctgg cctggggagg gcttgggcct gtgaggattc tccccgaagg
     4981 tgccctcgga ggcagcaggt ttgaggccct ggggggcccg ttccccagaa gctgccagtg
     5041 ctttcagatg cattgactct tcccgcctcc cctccctcag aaaggtagtg cccacactat
     5101 ttttaaaatg ttattttatg caatacactg ttcctagtga gcagggctct ggccctgagg
     5161 agtctttgca atgtattgaa ggaattgctg ccgtgtgagt tttgaatgat tttgcagtaa
     5221 agacctgatc tttctcatca
//
