LOCUS       NM_018049               1218 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens pleckstrin homology domain containing J1 (PLEKHJ1),
            transcript variant 2, mRNA.
ACCESSION   NM_018049
VERSION     NM_018049.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1218)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   4  (bases 1 to 1218)
  AUTHORS   Vinayagam,A., Stelzl,U., Foulle,R., Plassmann,S., Zenkner,M.,
            Timm,J., Assmus,H.E., Andrade-Navarro,M.A. and Wanker,E.E.
  TITLE     A directed protein interaction network for investigating
            intracellular signal transduction
  JOURNAL   Sci Signal 4 (189), rs8 (2011)
   PUBMED   21900206
REFERENCE   5  (bases 1 to 1218)
  AUTHORS   Barrios-Rodiles,M., Brown,K.R., Ozdamar,B., Bose,R., Liu,Z.,
            Donovan,R.S., Shinjo,F., Liu,Y., Dembowy,J., Taylor,I.W., Luga,V.,
            Przulj,N., Robinson,M., Suzuki,H., Hayashizaki,Y., Jurisica,I. and
            Wrana,J.L.
  TITLE     High-throughput mapping of a dynamic signaling network in mammalian
            cells
  JOURNAL   Science 307 (5715), 1621-1625 (2005)
   PUBMED   15761153
REFERENCE   6  (bases 1 to 1218)
  AUTHORS   Dresser,D.W., Jamin,S.P., Atkins,C.J. and Guerrier,D.
  TITLE     An expressed GNRP-like gene shares a bi-directional promoter with
            SF3A2 (SAP62) immediately upstream of AMH
  JOURNAL   Gene 277 (1-2), 163-173 (2001)
   PUBMED   11602354
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK001159.1, AK097723.1 and BQ013786.1.
            
            On Jun 28, 2019 this sequence version replaced NM_018049.2.
            
            Transcript Variant: This variant (2) contains an alternate 3'
            terminal exon, resulting in a novel 3' coding region and 3' UTR,
            compared to variant 1. It encodes isoform 2, which has a shorter
            and distinct C-terminus, compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC008966.2, AK001159.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2153932 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000326631.7/ ENSP00000318075.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-644               AK001159.1         1-644
            645-645             AK097723.1         1676-1676
            646-1215            AK001159.1         646-1215
            1216-1218           BQ013786.1         21-23               c
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.3"
     gene            1..1218
                     /gene="PLEKHJ1"
                     /gene_synonym="GNRPX"
                     /note="pleckstrin homology domain containing J1"
                     /db_xref="GeneID:55111"
                     /db_xref="HGNC:HGNC:18211"
                     /db_xref="MIM:617834"
     exon            1..175
                     /gene="PLEKHJ1"
                     /gene_synonym="GNRPX"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..531
                     /gene="PLEKHJ1"
                     /gene_synonym="GNRPX"
                     /note="isoform 2 is encoded by transcript variant 2;
                     guanine nucleotide releasing protein x; pleckstrin
                     homology domain-containing family J member 1; PH
                     domain-containing family J member 1"
                     /codon_start=1
                     /product="pleckstrin homology domain-containing family J
                     member 1 isoform 2"
                     /protein_id="NP_060519.1"
                     /db_xref="CCDS:CCDS12083.1"
                     /db_xref="GeneID:55111"
                     /db_xref="HGNC:HGNC:18211"
                     /db_xref="MIM:617834"
                     /translation="MRYNEKELQALSRQPAEMAAELGMRGPKKGSVLKRRLVKLVVNF
                     LFYFRTDEAEPVGALLLERCRVVREEPGTFSISFIEDPERKYHFECSSEEQCQEWMEA
                     LRRASYEFMRRSLIFYRNEIRKVTGKDPLEQFGISEEARFQLSGLQA"
     exon            176..243
                     /gene="PLEKHJ1"
                     /gene_synonym="GNRPX"
                     /inference="alignment:Splign:2.1.0"
     exon            244..310
                     /gene="PLEKHJ1"
                     /gene_synonym="GNRPX"
                     /inference="alignment:Splign:2.1.0"
     exon            311..401
                     /gene="PLEKHJ1"
                     /gene_synonym="GNRPX"
                     /inference="alignment:Splign:2.1.0"
     exon            402..465
                     /gene="PLEKHJ1"
                     /gene_synonym="GNRPX"
                     /inference="alignment:Splign:2.1.0"
     exon            466..1218
                     /gene="PLEKHJ1"
                     /gene_synonym="GNRPX"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1196..1201
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLEKHJ1"
                     /gene_synonym="GNRPX"
                     /note="hexamer: AATAAA"
     polyA_site      1218
                     /gene="PLEKHJ1"
                     /gene_synonym="GNRPX"
                     /note="major polyA site"
ORIGIN      
        1 gacatttttg gcgccggccc cagcctgagc ggggacggcg gccgggaggg cgcggcccgg
       61 gttcccgttc cccgcggagc catgcggtac aacgagaagg agctgcaggc tctgtcccgg
      121 cagccggccg agatggcggc cgagctgggc atgaggggcc ccaagaaggg cagcgtgctg
      181 aagcggcggc tggtgaagct ggtggtgaat ttcctcttct actttcggac agacgaggcc
      241 gagcccgtcg gagccctgct gctggagcgc tgcagagtcg tccgggaaga gcccggcacc
      301 ttctccatca gcttcattga ggaccctgag aggaagtatc actttgagtg cagcagcgag
      361 gagcagtgtc aggagtggat ggaggctctg cgtcgggcca gctacgagtt catgcggaga
      421 agcctcatct tctacaggaa cgaaatccgg aaggtgacgg gcaaggaccc cctggaacag
      481 ttcggcatat ccgaggaggc caggttccag ctgagtggct tgcaggcgtg agcgcagggc
      541 acggtggtca gcgtgcagcg ggacgggact ggccctgccc agccatgaat cgcttggcca
      601 tgcctggatc tgttttgttt tggtttttgg tttttgggtc agggtttcac tgtgttgccc
      661 aggctagagt gcagtggtgc cacagctcac tgtgaccttg accttctgga ctcaagtgat
      721 cctcctgcct cagcttccca agtagcgggg atcacaggca tgagccgcca cacccggcca
      781 tcacacctgg attttcagtg ggaggttttt ggtttggaga catccaaagc ctgaagccag
      841 gtgggtgtgg gcaggggctg cattttatga aactgcccag caagctgcgc tccctggggc
      901 cccaggatcc acctaactgg cctggcacct ggtgccacgt gctgctgccg ccaggatatg
      961 cgccttccca caggtgccct gcctgagttg tgtgcatcca ggggcctggt gagcccccag
     1021 gctggtggca tggcccccct gccccgtgct gaatgaatgt acagagccag acaaagctgt
     1081 gaatggccta ggggctgagt cccacaccag ctgtgaattc tcctgcagac aggagggccc
     1141 tggctgtgca cctggggaag tggttgccct ggggccaggg tgcttgttct gttcaaataa
     1201 aggtacctct tttccaca
//
