LOCUS       NM_052909              12591 bp    mRNA    linear   PRI 27-JUL-2024
DEFINITION  Homo sapiens pleckstrin homology and RhoGEF domain containing G4B
            (PLEKHG4B), mRNA.
ACCESSION   NM_052909 XM_006714446 XM_352571
VERSION     NM_052909.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12591)
  AUTHORS   Ninomiya,K., Ohta,K., Kawasaki,U., Chiba,S., Inoue,T., Kuranaga,E.,
            Ohashi,K. and Mizuno,K.
  TITLE     Calcium influx promotes PLEKHG4B localization to cell-cell
            junctions and regulates the integrity of junctional actin filaments
  JOURNAL   Mol Biol Cell 35 (2), ar24 (2024)
   PUBMED   38088892
REFERENCE   2  (bases 1 to 12591)
  AUTHORS   Benz,C., Ali,M., Krystkowiak,I., Simonetti,L., Sayadi,A.,
            Mihalic,F., Kliche,J., Andersson,E., Jemth,P., Davey,N.E. and
            Ivarsson,Y.
  TITLE     Proteome-scale mapping of binding sites in the unstructured regions
            of the human proteome
  JOURNAL   Mol Syst Biol 18 (1), e10584 (2022)
   PUBMED   35044719
REFERENCE   3  (bases 1 to 12591)
  AUTHORS   Ninomiya,K., Ohta,K., Yamashita,K., Mizuno,K. and Ohashi,K.
  TITLE     PLEKHG4B enables actin cytoskeletal remodeling during epithelial
            cell-cell junction formation
  JOURNAL   J Cell Sci 134 (2), 224080 (2021)
   PUBMED   33310911
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 12591)
  AUTHORS   Muller,P.M., Rademacher,J., Bagshaw,R.D., Wortmann,C., Barth,C.,
            van Unen,J., Alp,K.M., Giudice,G., Eccles,R.L., Heinrich,L.E.,
            Pascual-Vargas,P., Sanchez-Castro,M., Brandenburg,L., Mbamalu,G.,
            Tucholska,M., Spatt,L., Czajkowski,M.T., Welke,R.W., Zhang,S.,
            Nguyen,V., Rrustemi,T., Trnka,P., Freitag,K., Larsen,B., Popp,O.,
            Mertins,P., Gingras,A.C., Roth,F.P., Colwill,K., Bakal,C.,
            Pertz,O., Pawson,T., Petsalaki,E. and Rocks,O.
  TITLE     Systems analysis of RhoGEF and RhoGAP regulatory proteins reveals
            spatially organized RAC1 signalling from integrin adhesions
  JOURNAL   Nat Cell Biol 22 (4), 498-511 (2020)
   PUBMED   32203420
REFERENCE   5  (bases 1 to 12591)
  AUTHORS   Raffield,L.M., Cox,A.J., Hugenschmidt,C.E., Freedman,B.I.,
            Langefeld,C.D., Williamson,J.D., Hsu,F.C., Maldjian,J.A. and
            Bowden,D.W.
  TITLE     Heritability and genetic association analysis of neuroimaging
            measures in the Diabetes Heart Study
  JOURNAL   Neurobiol Aging 36 (3), 1602 (2015)
   PUBMED   25523635
REFERENCE   6  (bases 1 to 12591)
  AUTHORS   Luo,F.W., Luo,C.Q., Xie,J.S., Gen,Q., Liu,H., Li,F., Chen,W.B. and
            Wang,L.
  TITLE     [Analysis of copy number variations in an infant with Cri du Chat
            syndrome by array-based comparative genomic hybridization]
  JOURNAL   Zhonghua Yi Xue Yi Chuan Xue Za Zhi 30 (4), 443-446 (2013)
   PUBMED   23926013
REFERENCE   7  (bases 1 to 12591)
  AUTHORS   Dalla,E., Mignone,F., Verardo,R., Marchionni,L., Marzinotto,S.,
            Lazarevic,D., Reid,J.F., Marzio,R., Klaric,E., Licastro,D.,
            Marcuzzi,G., Gambetta,R., Pierotti,M.A., Pesole,G. and Schneider,C.
  TITLE     Discovery of 342 putative new genes from the analysis of
            5'-end-sequenced full-length-enriched cDNA human transcripts
  JOURNAL   Genomics 85 (6), 739-751 (2005)
   PUBMED   15885500
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DR033854.1, BG325888.1,
            AC010443.8, AB067496.2, AC021087.5 and AK125163.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_052909.4.
            
            Summary: This gene encodes a large protein that contains a
            pleckstrin homology domain and may function as a guanine nucleotide
            exchange factor. [provided by RefSeq, May 2017].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA2144120,
                              SAMEA2145240 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000637938.2/ ENSP00000490806.1
            RefSeq Select criteria    :: based on single protein-coding
                                         transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-220               DR033854.1         29-248
            221-688             BG325888.1         198-665
            689-1082            AC010443.8         11577-11970         c
            1083-3081           AB067496.2         1-1999
            3082-3143           AC021087.5         18680-18741
            3144-4358           AB067496.2         2062-3276
            4359-4466           AK125163.1         1106-1213
            4467-4497           AB067496.2         3385-3415
            4498-5131           AK125163.1         1449-2082
            5132-12591          AC021087.5         38097-45556
FEATURES             Location/Qualifiers
     source          1..12591
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5p15.33"
     gene            1..12591
                     /gene="PLEKHG4B"
                     /gene_synonym="ARHGEF48"
                     /note="pleckstrin homology and RhoGEF domain containing
                     G4B"
                     /db_xref="GeneID:153478"
                     /db_xref="HGNC:HGNC:29399"
                     /db_xref="MIM:620665"
     exon            1..109
                     /gene="PLEKHG4B"
                     /gene_synonym="ARHGEF48"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..4948
                     /gene="PLEKHG4B"
                     /gene_synonym="ARHGEF48"
                     /note="PH domain-containing family G member 4B; pleckstrin
                     homology domain containing, family G (with RhoGef domain)
                     member 4B"
                     /codon_start=1
                     /product="pleckstrin homology domain-containing family G
                     member 4B"
                     /protein_id="NP_443141.4"
                     /db_xref="CCDS:CCDS34124.2"
                     /db_xref="GeneID:153478"
                     /db_xref="HGNC:HGNC:29399"
                     /db_xref="MIM:620665"
                     /translation="MGFSTADGGGGPGARDLESLDACIQRTLSALYPPFEATAATVLW
                     QLFSVAERCHGGDGLHCLTSFLLPAKRALQHLQQEACARYRGLVFLHPGWPLCAHEKV
                     VVQLASLHGVRLQPGDFYLQVTSAGKQSARLVLKCLSRLGRGTEEVTVPEAMYGCVFT
                     GAFLEWVNRERRHVPLQTCLLTSGLAVHRAPWSDVTDPVFVPSPGAILQSYSSCTGPE
                     RLPSSPSEAPVPTQATAGPHFQGSASCPDTLTSPCRRGHTGSDQLRHLPYPERAELGS
                     PRTLSGSSDRDFEKVSPSEQGPRMPPENCGGSGERPDPMDQEDRPKALTFHTDLGIPS
                     SRRRPPGDPTCVQPRRWFRESYMEALRNPMPLGSSEEALGDLACSSLTGASRDLGTGA
                     VASGTQEETSGPRGDPQQTPSLEKERHTPSRTGPGAAGRTLPRRSRSWERAPRSSRGA
                     QAAACHTSHHSAGSRPGGHLGGQAVGTPNCVPVEGPGCTKEEDVLASSACVSTDGGSL
                     HCHNPSGPSDVPARQPHPEQEGWPPGTGDFPSQVPKQVLDVSQELLQSGVVTLPGTRD
                     RHGRAVVQVRTRSLLWTREHSSCAELTRLLLYFHSIPRKEVRDLGLVVLVDARRSPAA
                     PAVSQALSGLQNNTSPIIHSILLLVDKESAFRPDKDAIIQCEVVSSLKAVHKFVDSCQ
                     LTADLDGSFPYSHGDWICFRQRLEHFAANCEEAIIFLQNSFCSLNTHRTPRTAQEVAE
                     LIDQHETMMKLVLEDPLLVSLRLEGGTVLARLRREELGTEDSRDTLEAATSLYDRVDE
                     EVHRLVLTSNNRLQQLEHLRELASLLEGNDQQSCQKGLQLAKENPQRTEEMVQDFRRG
                     LSAVVSQAECREGELARWTRSSELCETVSSWMGPLDPEACPSSPVAECLRSCHQEATS
                     VAAEAFPGAGVAVLKPHALGKPWASQQDLWLQYPQTRLRLEEALSEAAPDPSLPPLAQ
                     SPPKHERAQEAMRRHQKPPSFPSTDSGGGAWEPAQPLSGLPGRALLCGQDGETLRPGL
                     CALWDPLSLLRGLPGAGATTAHLEDSSACSSEPTQTLASRPRKHPQKKMIKKTQSFEI
                     PQPDSGPRDSCQPDHTSVFSKGLEVTSTVATEKKLPLWQHARSPPVTQSRSLSSPSGL
                     HPAEEDGRQQVGSSRLRHIMAEMIATEREYIRCLGYVIDNYFPEMERMDLPQGLRGKH
                     HVIFGNLEKLHDFHQQHFLRELERCQHCPLAVGRSFLRHEEQFGMYVIYSKNKPQSDA
                     LLSSHGNAFFKDKQRELGDKMDLASYLLRPVQRVAKYALLLQDLLKEASCGLAQGQEL
                     GELRAAEVVVCFQLRHGNDLLAMDAIRGCDVNLKEQGQLRCRDEFIVCCGRKKYLRHV
                     FLFEDLILFSKTQKVEGSHDVYLYKQSFKTAEIGMTENVGDSGLRFEIWFRRRRKSQD
                     TYILQASSAEVKSAWTDVIGRILWRQALKSRELRIQEMASMGIGNQPFMDVKPRDRTP
                     DCAVISDRAPKCAVMSDRVPDSIVKGTESQMRGSTAVSSSDHAAPFKRPHSTISDSST
                     SSSSSQSSSILGSLGLLVSSSPAHPGLWSPAHSPWSSDIRACVEEDEPEPELETGTQA
                     AVCEGAPAVLLSRTRQA"
     exon            110..307
                     /gene="PLEKHG4B"
                     /gene_synonym="ARHGEF48"
                     /inference="alignment:Splign:2.1.0"
     exon            308..1541
                     /gene="PLEKHG4B"
                     /gene_synonym="ARHGEF48"
                     /inference="alignment:Splign:2.1.0"
     exon            1542..1751
                     /gene="PLEKHG4B"
                     /gene_synonym="ARHGEF48"
                     /inference="alignment:Splign:2.1.0"
     exon            1752..1875
                     /gene="PLEKHG4B"
                     /gene_synonym="ARHGEF48"
                     /inference="alignment:Splign:2.1.0"
     exon            1876..1969
                     /gene="PLEKHG4B"
                     /gene_synonym="ARHGEF48"
                     /inference="alignment:Splign:2.1.0"
     exon            1970..2056
                     /gene="PLEKHG4B"
                     /gene_synonym="ARHGEF48"
                     /inference="alignment:Splign:2.1.0"
     exon            2057..2173
                     /gene="PLEKHG4B"
                     /gene_synonym="ARHGEF48"
                     /inference="alignment:Splign:2.1.0"
     exon            2174..2272
                     /gene="PLEKHG4B"
                     /gene_synonym="ARHGEF48"
                     /inference="alignment:Splign:2.1.0"
     exon            2273..2412
                     /gene="PLEKHG4B"
                     /gene_synonym="ARHGEF48"
                     /inference="alignment:Splign:2.1.0"
     exon            2413..2551
                     /gene="PLEKHG4B"
                     /gene_synonym="ARHGEF48"
                     /inference="alignment:Splign:2.1.0"
     exon            2552..2713
                     /gene="PLEKHG4B"
                     /gene_synonym="ARHGEF48"
                     /inference="alignment:Splign:2.1.0"
     exon            2714..3540
                     /gene="PLEKHG4B"
                     /gene_synonym="ARHGEF48"
                     /inference="alignment:Splign:2.1.0"
     exon            3541..3793
                     /gene="PLEKHG4B"
                     /gene_synonym="ARHGEF48"
                     /inference="alignment:Splign:2.1.0"
     exon            3794..3883
                     /gene="PLEKHG4B"
                     /gene_synonym="ARHGEF48"
                     /inference="alignment:Splign:2.1.0"
     exon            3884..4114
                     /gene="PLEKHG4B"
                     /gene_synonym="ARHGEF48"
                     /inference="alignment:Splign:2.1.0"
     exon            4115..4285
                     /gene="PLEKHG4B"
                     /gene_synonym="ARHGEF48"
                     /inference="alignment:Splign:2.1.0"
     exon            4286..4466
                     /gene="PLEKHG4B"
                     /gene_synonym="ARHGEF48"
                     /inference="alignment:Splign:2.1.0"
     exon            4467..4628
                     /gene="PLEKHG4B"
                     /gene_synonym="ARHGEF48"
                     /inference="alignment:Splign:2.1.0"
     exon            4629..12591
                     /gene="PLEKHG4B"
                     /gene_synonym="ARHGEF48"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7498..7503
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLEKHG4B"
                     /gene_synonym="ARHGEF48"
                     /note="hexamer: AATAAA"
     polyA_site      7527
                     /gene="PLEKHG4B"
                     /gene_synonym="ARHGEF48"
                     /note="major polyA site"
     regulatory      12571..12576
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLEKHG4B"
                     /gene_synonym="ARHGEF48"
                     /note="hexamer: CATAAA"
     polyA_site      12591
                     /gene="PLEKHG4B"
                     /gene_synonym="ARHGEF48"
ORIGIN      
        1 agtgcacagc gggaccaggc agagttcggg gaaagcgtcg gagttcggga gaccagggtc
       61 cagcatgggt ttcagcacag cagacggcgg gggcggccca ggcgcccggg atctggaatc
      121 tcttgatgcc tgtatccaga ggacgctctc tgccttgtac ccaccgtttg aagccacggc
      181 agccacggtg ctctggcagc tgttcagcgt ggccgagagg tgccacggtg gggacgggct
      241 gcactgcctc accagcttcc tcctcccagc caagagggcc ctgcagcacc tgcagcagga
      301 agcctgtgcc aggtacaggg gtctggtctt cctgcaccca ggctggccgc tgtgcgccca
      361 tgagaaggtg gtggtgcagc tggcgtccct gcacggagtc aggctccagc ccggggactt
      421 ctacctgcag gtcacgtcgg cggggaagca gtcagctaga ctggtcttga aatgcctgtc
      481 ccggctggga agaggcacag aggaagtcac cgtccctgag gccatgtatg gctgtgtctt
      541 cacgggggcg ttcctggagt gggtgaaccg ggagcggcgc catgtccccc tgcaaacctg
      601 cttgctgacc tcaggcttgg ccgtccaccg agccccgtgg agcgacgtca ctgaccctgt
      661 ctttgtcccc agccctggag ccatcctgca gagctactcc agctgcacag gtcctgagcg
      721 gctgcccagc agcccctcag aggccccagt ccccacccaa gccacagcag gcccccattt
      781 ccagggaagc gcctcttgcc ccgacaccct gacctcaccc tgccgccgag ggcatacggg
      841 cagcgaccag ctcaggcacc ttccttatcc agaaagagcc gagctgggaa gccccaggac
      901 cctgtctgga agctcagaca gggacttcga aaaggtcagc ccctcagagc agggcccacg
      961 gatgccccct gagaactgtg gggggtcggg ggagaggccg gaccccatgg accaggagga
     1021 cagacccaag gccctcacct tccacacaga cctgggcatc ccgagcagca ggaggcggcc
     1081 gccgggggac cccacttgtg tgcagcctag acgctggttc agggagtcgt acatggaagc
     1141 cttgcggaac cccatgcccc tgggcagctc tgaggaggcc ctcggggacc tggcctgcag
     1201 ctccctgact ggagccagca gggacctggg gactggggca gtagccagtg ggacccagga
     1261 ggaaacctct ggcccccggg gagaccccca acagacccca agtctagaga aggagaggca
     1321 cacacccagc cggacaggtc caggagctgc agggcggact cttcccagga gatctcggtc
     1381 ctgggaaagg gcacccagaa gctccagagg ggcccaggct gcagcctgcc acacctccca
     1441 ccactcagca ggctccaggc ctgggggcca cctaggagga caagctgtgg ggaccccaaa
     1501 ctgtgtccca gtagagggtc ccggctgcac caaagaggaa gacgttcttg catcctcagc
     1561 ctgtgtcagc acagacggcg gcagcctcca ttgccacaac cccagcgggc cttccgatgt
     1621 gcctgcccgg cagccacacc ccgagcaaga agggtggcca cccggcacag gagacttccc
     1681 cagccaggtg cccaagcagg tgctggacgt cagtcaggag ctgctgcagt ccggggtcgt
     1741 caccctccca gggacccgag accgtcatgg cagagcagtg gtgcaggtcc gcaccaggag
     1801 cctgctctgg accagggaac actcgtcctg tgctgagctg acccgcctgc tgctgtactt
     1861 ccatagcatc cccaggaaag aggtccggga cctggggctg gttgtcctgg tggatgcacg
     1921 caggagtcca gctgcccctg ccgtctccca ggccctctca ggattgcaga acaacacatc
     1981 tcctataatt catagtatct tgctgttggt agataaagaa tctgcattta ggcctgacaa
     2041 ggatgcaata attcagtgtg aggtcgtgag ctccctgaag gccgtgcaca aatttgttga
     2101 cagctgccag ctgaccgcag acctcgacgg ctcctttccc tacagccatg gtgactggat
     2161 ctgcttccgt cagaggctgg aacacttcgc tgcaaactgt gaagaagcca tcattttcct
     2221 acagaattca ttctgctccc tgaacaccca cagaacccca agaacagccc aggaagtcgc
     2281 cgagttaatt gaccagcatg agacgatgat gaagcttgtc ctggaagacc cactgcttgt
     2341 gtctctcagg ctggaggggg gcaccgtcct ggcgcggctg aggagagaag agcttggcac
     2401 agaagacagc cgggacaccc tggaggccgc cacaagcctg tacgaccgag tggatgagga
     2461 ggtgcacagg ctggtcctca cctcgaacaa tcgtctccag cagctggagc acctccggga
     2521 gctggcgtca ctcctggaag ggaatgacca gcaatcctgc cagaaaggac tacagctggc
     2581 gaaggagaac ccgcaacgta cagaggaaat ggtccaggat ttcagaaggg gcctgagcgc
     2641 cgtggtcagc caggctgagt gcagggaggg agagctggcc aggtggaccc gctcgtccga
     2701 gttgtgcgag acggtgagca gctggatggg gcccctggac ccggaggctt gtccctcctc
     2761 acccgtggct gagtgtttga ggagctgtca ccaggaggct acctcggtgg ctgcagaggc
     2821 cttccccggg gcaggtgtgg cagtgctgaa gcctcatgcc ctggggaaac cgtgggcatc
     2881 acagcaagac ctgtggctgc agtaccccca gacccggctc cgtctggaag aggccctttc
     2941 tgaggctgcc ccagacccca gcttaccgcc ccttgcccag agccccccaa agcatgagcg
     3001 tgcccaggag gccatgagga ggcaccagaa gccaccctca ttccccagca cggacagtgg
     3061 gggtggtgcc tgggaacctg cgcaaccact gtccggcctc cctggacgag cgcttctgtg
     3121 tggacaggac ggggagaccc tgcgcccagg gctgtgtgct ctgtgggacc cactgtccct
     3181 cctcaggggc cttccagggg caggggccac cacggcccac ctggaggaca gctctgcctg
     3241 ttcctctgag cccacccaga ccctggccag ccgccccagg aaacatcccc agaagaaaat
     3301 gataaagaaa acgcaaagtt tcgagatacc tcagcccgac agtggcccca gggactcctg
     3361 ccagccagac catactagtg tcttcagcaa gggcctggag gtaaccagca ctgtagccac
     3421 agagaagaag ctcccgctgt ggcagcatgc caggagcccc ccggtcactc agagccggag
     3481 tctgtcctcc ccctcggggc tccaccctgc tgaggaggat gggaggcagc aggtgggcag
     3541 cagccgactg aggcacatca tggccgagat gatcgccaca gagagggagt acattcggtg
     3601 cttaggatac gtcattgaca actattttcc agaaatggaa agaatggact tgccccaggg
     3661 ccttcgaggg aagcaccacg ttattttcgg caacttggag aagctccacg acttccacca
     3721 gcagcacttc ctccgggagc tggagcgctg ccagcactgc cccttggccg tgggccgcag
     3781 tttcctgaga cacgaagagc agtttgggat gtacgtgatc tacagcaaaa acaagccgca
     3841 gtcggatgcc ctgctcagca gccatggcaa cgccttcttc aaggacaagc agcgggagct
     3901 aggtgacaaa atggacctgg cctcctacct gctgcggccc gtgcagcgtg tggccaagta
     3961 cgcgctgcta ctccaggacc tgctcaagga ggccagctgt ggcctggccc aggggcagga
     4021 gctgggcgag ctccgagccg ccgaggtcgt ggtctgcttc cagctgcgtc acggcaatga
     4081 cctgctggcc atggacgcca tccgcggctg tgacgtgaat ttgaaggaac aggggcagct
     4141 gagatgccgg gatgagttta tcgtttgctg cgggaggaag aagtatctga ggcatgtgtt
     4201 cctctttgaa gacctcatcc tgtttagcaa gacccagaag gtggagggca gccacgacgt
     4261 ctacctgtac aagcagtcct tcaagacggc cgagatcggg atgacagaga acgtcgggga
     4321 cagtggcttg aggtttgaga tttggtttcg caggcggcgg aaatctcagg acacctacat
     4381 tctccaagca agctcggcag aggtcaagag tgcatggacc gatgtcatag ggaggatcct
     4441 gtggcggcag gcactaaaga gcagagaact cagaatccaa gaaatggcat ccatgggtat
     4501 aggcaaccag ccattcatgg atgtcaagcc cagagaccgg acccctgact gtgcagtgat
     4561 aagcgaccgg gctcccaaat gtgcagtgat gagcgaccga gtccccgaca gcatcgtcaa
     4621 gggcacagag tcacaaatga gagggtccac agcggtgtcc tcctctgacc acgccgcccc
     4681 cttcaagcga ccacactcca ccatctcaga cagcagcacc tcctcttcta gcagccagtc
     4741 ctcctccatc ctggggtcgc tgggcctgct tgtgtcctcc agcccagccc acccgggcct
     4801 atggagccct gcccacagcc cctggtcatc tgatatcaga gcctgcgtcg aggaagatga
     4861 gccagagcca gaactagaga cgggcaccca ggctgcagtg tgtgaggggg ctcctgctgt
     4921 gctgctgagc cgcacacgcc aggcctgatg actgtcaggg tggcagtgcc catcatgtgg
     4981 ctagaacaat acagagggag cagcacgcca ggcctgatga ctctgggggt ggcggtgccc
     5041 atcgcgtggc tggaacgatc cagagggaat agcacagcag gtgtccaggt atttcccagg
     5101 attttagaca ttccctaaca ttttcaaaca aatttataat tttgtcttat ttaaaaaaca
     5161 aaccttccac ttccacccaa gacaacagca taggaaacag acctaaaaca agacaaaaaa
     5221 agactaaaca tgaaacaaaa gatgtcaaga cacaagacat ttggcaacaa aggaccgcga
     5281 tccctgagag acaggaaaac gggaggtgag cccggtgatg gcctcagacc tccctccgcc
     5341 ttgagagttt gcaggcctca gtgcagggag gggcccagga agagcccagc agcctccgtg
     5401 agttgaagag accaagtcag caggagttgg caggtcagag tgccagagag agatgcctgc
     5461 atggggaaag actcaccaga tcagcccctc cagcacctag tgggttgcta cccattcgca
     5521 cactcaaaca gggaggctac caaggctggg agcaaccttt gaaaagacca aagatcaatt
     5581 cctggtactc atgtgggctg gggacaggac ccgttcccac cagccagact agggaaactc
     5641 tcaatagatc aattcctgat actcatgtgg gctggggaca ggacccactc ccaccagcca
     5701 gactaggaaa actcccaatc tgtgagcatt aggtggagtt ctcagaaaat cttgcctcag
     5761 gaatggggat aattagccct agactgagcc ctgctcctga ctcgcctaca aaatcataaa
     5821 agcaagacct aaaatgatca aaccattccc agataattca ctttatccca gaacaaagct
     5881 ccagatgatc taggggttac caaagtgtcc acaccctgca aggtaaactc acagtgcctg
     5941 acctcggagt gccaggcgtg cagaggagca gccaagggca aacactccaa ggagagtcgc
     6001 ctggcaaagc caaccaccca gagccagcgt ctgtgctaga atcacagaat tcaaactgtg
     6061 gctgaaatcg cagcccaggc gtccagaaag ttaaagttaa gaacctataa tcccagcact
     6121 ttgggaggcc gaggcaggcg gatcacgagg tcaggagatc aagaccatcc tggctaacat
     6181 ggtgaaaccc cgcctactaa aaatacaaaa aattagccgg gcatggtgtt ggccgcctgt
     6241 agtcccagct acttgggagg ctgaggcagg agaatggcat gaacccggga ggcggagttt
     6301 gcagtgagct gagatcacgc cactgcactc cagcctgggc gacagagcaa gactctgtct
     6361 cacaaaaaag gttaagaaat gacttaatgg aagagattca gaagacccag accgacctta
     6421 cagggatgag aaactacaat attgacagtg gaaactatcc tggatggggt aacagattgg
     6481 atttcacaga agagaccatg aggcaccttg aagacacatc agtggaaact atttgcattt
     6541 gttgggcctc cagagagaca gaaccaatag gagatagcta gatatatata cagacagata
     6601 gatagataga tagatcgata gatagataga tagatagata gatagataga tagactgaca
     6661 gacagatgag aggggcctta ggggcattgg ctgacacaat tttggcaggt caagagcccc
     6721 acaataggcc atccacaagc tggagaccca aggaagctgc tagtgtgccc agtccgagtc
     6781 ctaaagcctc agagccaggg aagccagtgg tgtaattctc agaggccaaa ggcctgagaa
     6841 cctgggggcc actggtataa gtctgagtcc atagacccag tgaactccaa acacaagaga
     6901 tacgagtgca catcataatc aaattgcata taaccagtga taaacagaaa atcctaaaac
     6961 cagtcagagg acaagggcat gttctgttgg gaggcacaaa gctgatgagg atggctgact
     7021 cctcaggagc agcatgggtg agaggcagtg gaatgtacta aggagaaaac tgccaaattc
     7081 acattctgta cctgacaaaa tctttccgaa gcgatggtga cataaagaca ttttcataca
     7141 cacagaaact aagagtttca tccccagcag acctgcacta caagaaatgt taaatgatgt
     7201 gcttcaggga aaagaaaaat gaagagcatg tgcaccatcc aggattttcc tcgttattta
     7261 aatcccttta gcccatctcc catttgcccc aaggaatgag tgctggcagt gagctgcact
     7321 ttttttttct ctaaacggga aatgggttaa aagatgagtg gcttggccgg gtgcggtggc
     7381 tcatgtctct aatcccagca ctttgggagg ctgaggccag aggatcactt gacttgagcc
     7441 caggaactgg ggggtcatgc tgggaaacat ggcaagaccg catctctatt aaaaataaat
     7501 aaaagttaac tgactgttta aacaaaagta ttaacgtatc tgcggtttgt actatacgca
     7561 caggtaaact gtgtgaccac agcagcgtga tggctgggag acgctggatg tgatacgtga
     7621 agtgaggggt atcacttgaa ggtagattgt ggtaagctaa agatgtactg ataaaccctt
     7681 gaaacaccca ctaacaaaga attacagcta atagaccaac agaggagata aaatggaatt
     7741 tttaaaaatc cagtccaaaa atacgtagag aaagagggaa agggaagaat ggacttgggg
     7801 gcgacacaga agacaagtgg agagagactg aagcagccac tggccatcac agcaaacaca
     7861 agcagggcgc aggacgccgg caagccacag acaggcctgc tctctgaatt ggtgaccaca
     7921 tgagtaactt cacgggtctg ttctatgtcc agagttgtca aactgcatgc tttaaagatg
     7981 tgcagtggat cgtatgtcgc ttaaatccca acaaagcagt atttttttaa agtacaatca
     8041 tgcacacagc acttcccctg ggaggcaggg gctgtgccca cagaagtcgt tacctctgcg
     8101 tcctgagtat ggacagccac catcacacag ccccagaggc cgcccaggct ctagccacac
     8161 tctggcactg cctgggacag gtgccctcag gaacccaaat gccactgccc ccgtgaaagc
     8221 cacttcaagc gtgttgcttc aaatcagaag gcatctacgc ctgtggaaga ggaggctgct
     8281 gggggcccag gtgtgcaggg ggcagggcca ggtgcagggg cacagcttat gcctgtggcc
     8341 aacccactgg ggagcaaggc tggggatatg cccaggccag cagggccagc aggagtgact
     8401 gcgccatcct gaacacgtcc ttgggcctcc caggaccttg gtgcctccca tctgggctgg
     8461 cacaagcctc cagtcaaaag atgggtctct ctctcctgat agcatggagc ctggatgtgg
     8521 gaatggagcc acaccagccc cacgggagcc ccacgaccca tcgggctgtg ggatgcatcc
     8581 ccacggccgg gacccctcag cctgtgactt ggtctgggca gatgcaaagg ccaccctgca
     8641 gggctgaggt gtctgtgggg aaggaggtgc ccagccctgg gaggccatct tgctcctgct
     8701 ttttatttaa cccatggggc tctttggcct cattttctgt tttttgagtt tctcgtgcac
     8761 tccaaactgt cctggagcca gccctggtgt gaaacagagg cttcctgctg gcagaagagg
     8821 cccaccgagt tacgtccaca ccagctggcc ctgggcacct ctctctctgg ccttctgtcc
     8881 acactgggat ttggctctga tttccagaag gcagggttag gaaacacctg tttccaggca
     8941 gagtaatgct tctgtctgaa tcaggcctca tgggcttcac cagcagacac gaaggtttca
     9001 tttagatggc gaacacgtgt tctcatttct ccagggttct ggacccgtga gctgaagcag
     9061 gtgctgagaa gtgggagagg cggccactgg gtccctcagg gtcactcacc ccgccacgtg
     9121 aggccacttc cgcccagcac tgggggcgct gggagctgag gggcccacag gggacgtggg
     9181 agacagcccc gatgcgtccc catccgcagt gacgctagcg cggagtcgtc gccaagcacg
     9241 tggagggaca gccctggagc cctggcctgg cccagcatct cccagagtca cttcctgatg
     9301 cctccccagc cctgcccagg aagggtttgt ggccacagcc ccacagcggc cccctcgcct
     9361 cgaccaccag tgcctccttc caagctgcct gagcaatcct gggtggtgag ctccaccact
     9421 cccacccacg cctcagcact gggacccccc agggatggtg tggggattcc tgccccccgt
     9481 ccatgcctca gcactgggag ctgggaaggt gtgtgatttc ctcccccacc tcagtactgg
     9541 gacccccagg aagggtgtgt ggccccacca tcctgaatgc tcagggctgg tggtcagcga
     9601 ggtggtttct aaaccaagcc ctgggaggag ccatcctggg actgtgcttg gacaaggttg
     9661 gggctcccgc agtgagctct agccctcccc aggaccctcc cacggcccca gggtgccggc
     9721 cttcagattg accccattcg tggatgagtc tacaaggctc cccagtgccc agcaagagtg
     9781 tttttacatt cagaggtgag gaaggggtgc cggcatctgc cccagacaca accgggcagg
     9841 tacagatgtc atccccttga ggacagcagg gaggcgactc aggagaggct gatcagacgg
     9901 gagccgcatc cccatcacct tccagagaga cctcttcttt tgccaggtgt cgtgagggct
     9961 gtggcttagc tgggcctgcg tccatccccg gttctgggga gatgcccggg cccctcctgg
    10021 atggcagcag cagtggaggt ttgggttttg agggagccct aagaacctga gagacttaga
    10081 tggagcatgg cacgccctgg agagtccacg tccccagacc cctcatgcca acaccattcc
    10141 acccagcagt ttcttccctg tggtcagaac aaaaccccca agcctcaggc ctcaggctgg
    10201 ttttctgaca ggcaaaatgc tccttacata aggaaagacc tttccttggc tgaccctaaa
    10261 gtagccaagg ggagctcaca tcgcctggcc tgccgccacc accaggctgc ctgaccagct
    10321 caccgccttg ttctagatgc ttctgaagca acagctcgcc tggccccacc cccatcaagc
    10381 atctgctggg cagcctccct ccccggcccg cctgtagctc ccaaaggtct caagcctcaa
    10441 catcctggta accaagtgca cccagagggc tggcagggtc acatggaggc aggggaccct
    10501 tggccccagg gtggtcgtct tcccaggagg tccgcctgtc aggtcagagg gcagcaggag
    10561 gccaccccac ctccccgggt tccctcgagc attcctttgt caggagcagc tcccccagga
    10621 tccaggcatc cctggccccc ctttgccccc gccctgctct caggagcccc ctgccaggtg
    10681 ctctgaccac aggaaacagg agccctcagg gccactgcca gaggacggat tctgctgcca
    10741 accagaggtc ctggggctgg cggcgctggt gtgcctgccc tgggccttca gcagccccca
    10801 taaggagaaa gctccagcct cccgttcaga gaaggctcca tcctggggac acgcctctcc
    10861 caaggtcacc cacgtggggg ctttgttact catgcttccc agactgcgtg ctgtgtcaga
    10921 gttttcacac ctgcctgatg tcctgggggg gctcacagag gaccgaccct gcagggagat
    10981 gctgacccac cctgagaccc aggagaagaa aacggccagg gtccaggctg ctcccaccat
    11041 ccctggggga ttagcacctt ggctgagccc gaactgacca cctggggcgt aggtccaaga
    11101 tttaattaca aaacgatagg tgaatgtggg ttctgtgtgc ctgtgacata gagaatgttt
    11161 tccacaccgc gtgtgaaatg acgagagctt ttgtgtcaca catgtagctg ctacgagaca
    11221 gacgctcttg ttccggatca gagcagcaga cagaaccggc acttttaggg tccaaaccct
    11281 gttgcccaca gaacgcccat gacaccagct gggtggagct gccgccgagt ccacgccccg
    11341 tctggctggg acctggagct cccctcaagc tgcagatcca ctctgcacca caaggtggcc
    11401 tcgcagagga gcctcccgag ctttggaacc agaggcaggc tcagcgatgc tctccatccg
    11461 gcctggcccc accgtgtgta tcaggtgtca gaggccttgc tgggtgccag gccacgtctg
    11521 tgctgtcaca gctttgaggg gccagcgggt cctagggaga ggcccgtgcg acgtgagtaa
    11581 cagcagtttt tcttctgaca gctgggatgg aggtgggtcc tccagggagt gccccagtcc
    11641 tcgagagcca gacttgtgca cgggcctgga aatgaaggtc tcaggcaggg gctgcgcaca
    11701 ccgatggtca gcattgccgt tcggggcctg cagtcgccca taatgggccc tgccccctag
    11761 gcctggcctc ttttgctttc ctcctggatg gttcctctac ccctggtcca gcagggactc
    11821 agcagtgcct gccagctgca gacccccatg ctcctgcagc ctggaaccct cctgccctcc
    11881 aaaggtcatg gggtgaccca gggtgggaac accagacccc ctcaccccgt gtccaccgcc
    11941 catggccggg ctcacagtgt ctcagagccg tggtgagcca tcctaggacc agtgtacctc
    12001 acaacctttc agaccagtga aggccagtgg gctgggtggt ccaggcatgt ccccaacgca
    12061 ggtccagcca tctctgcaca gaactggaat ctcggttcat ttcggtttca gcaggtccca
    12121 tgtagttagc aggacagcac ttgagttagg tctgagaaag aacgcctgtt tgtcatggca
    12181 tttcctgtaa ggagcccaga gccgtggccc caggttggga cggcggcgcc agagctgggc
    12241 ctgagtctga cccaacctgt gctgagtggt cctgctggaa tgggaacccc aagaggacca
    12301 cccctctcta aaagaaggga aaacagtaag cgagagggag gctgcagggg cccagacctt
    12361 ggctgacctt cctagccatg gtctccaccc tccgagaccc aggggccccg cagtgacagc
    12421 agggtgttgg gccacacaga gcccgagtag gtaaaggaag gttctggaaa ccacttttca
    12481 gaacccaaaa ggtgtctggg aaatggcgtt acctgccact gttgttaagt gtttactttg
    12541 tatcaatact gaattgttaa aaattttaat cataaaaacc aatggaacat g
//
