LOCUS       NM_001129729            4512 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens pleckstrin homology and RhoGEF domain containing G4
            (PLEKHG4), transcript variant 4, mRNA.
ACCESSION   NM_001129729
VERSION     NM_001129729.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Yuan,Q., Fan,Y., Liu,Z., Wang,X., Jia,M., Dong,Y., Geng,Z.,
            Zheng,J. and Lu,X.
  TITLE     Pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4) leads
            to the activation of RhoGTPases promoting the malignant phenotypes
            of thyroid cancer
  JOURNAL   Apoptosis 28 (9-10), 1315-1331 (2023)
   PUBMED   37336836
  REMARK    GeneRIF: Pleckstrin homology and RhoGEF domain containing G4
            (PLEKHG4) leads to the activation of RhoGTPases promoting the
            malignant phenotypes of thyroid cancer.
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 4512)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 4512)
  AUTHORS   Takenaka,N., Yasuda,N., Nihata,Y., Hosooka,T., Noguchi,T., Aiba,A.
            and Satoh,T.
  TITLE     Role of the guanine nucleotide exchange factor in Akt2-mediated
            plasma membrane translocation of GLUT4 in insulin-stimulated
            skeletal muscle
  JOURNAL   Cell Signal 26 (11), 2460-2469 (2014)
   PUBMED   25025572
  REMARK    GeneRIF: Role of the guanine nucleotide exchange factor in
            Akt2-mediated plasma membrane translocation of GLUT4 in
            insulin-stimulated skeletal muscle.
REFERENCE   5  (bases 1 to 4512)
  AUTHORS   Gupta,M., Kamynina,E., Morley,S., Chung,S., Muakkassa,N., Wang,H.,
            Brathwaite,S., Sharma,G. and Manor,D.
  TITLE     Plekhg4 is a novel Dbl family guanine nucleotide exchange factor
            protein for rho family GTPases
  JOURNAL   J Biol Chem 288 (20), 14522-14530 (2013)
   PUBMED   23572525
REFERENCE   6  (bases 1 to 4512)
  AUTHORS   Ishikawa,K., Toru,S., Tsunemi,T., Li,M., Kobayashi,K., Yokota,T.,
            Amino,T., Owada,K., Fujigasaki,H., Sakamoto,M., Tomimitsu,H.,
            Takashima,M., Kumagai,J., Noguchi,Y., Kawashima,Y., Ohkoshi,N.,
            Ishida,G., Gomyoda,M., Yoshida,M., Hashizume,Y., Saito,Y.,
            Murayama,S., Yamanouchi,H., Mizutani,T., Kondo,I., Toda,T. and
            Mizusawa,H.
  TITLE     An autosomal dominant cerebellar ataxia linked to chromosome
            16q22.1 is associated with a single-nucleotide substitution in the
            5' untranslated region of the gene encoding a protein with spectrin
            repeat and Rho guanine-nucleotide exchange-factor domains
  JOURNAL   Am J Hum Genet 77 (2), 280-296 (2005)
   PUBMED   16001362
  REMARK    GeneRIF: puratrophin-1 has a role in intracellular signaling and
            actin dynamics at the Golgi apparatus
REFERENCE   7  (bases 1 to 4512)
  AUTHORS   Hirano,R., Takashima,H., Okubo,R., Tajima,K., Okamoto,Y.,
            Ishida,S., Tsuruta,K., Arisato,T., Arata,H., Nakagawa,M., Osame,M.
            and Arimura,K.
  TITLE     Fine mapping of 16q-linked autosomal dominant cerebellar ataxia
            type III in Japanese families
  JOURNAL   Neurogenetics 5 (4), 215-221 (2004)
   PUBMED   15455264
  REMARK    GeneRIF: the autosomal dominant cerebellar ataxia that we have
            characterized is allelic with SCA4 and Japanese 16q-linked ADCA
            type III.
REFERENCE   8  (bases 1 to 4512)
  AUTHORS   Brusco,A., Gellera,C., Cagnoli,C., Saluto,A., Castucci,A.,
            Michielotto,C., Fetoni,V., Mariotti,C., Migone,N., Di Donato,S. and
            Taroni,F.
  TITLE     Molecular genetics of hereditary spinocerebellar ataxia: mutation
            analysis of spinocerebellar ataxia genes and CAG/CTG repeat
            expansion detection in 225 Italian families
  JOURNAL   Arch Neurol 61 (5), 727-733 (2004)
   PUBMED   15148151
  REMARK    GeneRIF: Observational study of genotype prevalence. (HuGE
            Navigator)
REFERENCE   9  (bases 1 to 4512)
  AUTHORS   Hellenbroich,Y., Bubel,S., Pawlack,H., Opitz,S., Vieregge,P.,
            Schwinger,E. and Zuhlke,C.
  TITLE     Refinement of the spinocerebellar ataxia type 4 locus in a large
            German family and exclusion of CAG repeat expansions in this region
  JOURNAL   J Neurol 250 (6), 668-671 (2003)
   PUBMED   12796826
  REMARK    GeneRIF: Spinocerebellar ataxia type 4 (SCA4) is mapped to
            chromosome 16q22.1 in northern germany.Haplotype analyses refined
            the gene locus to a 3.69 cM interval between D16S3019 and D16S512.
REFERENCE   10 (bases 1 to 4512)
  AUTHORS   Flanigan,K., Gardner,K., Alderson,K., Galster,B., Otterud,B.,
            Leppert,M.F., Kaplan,C. and Ptacek,L.J.
  TITLE     Autosomal dominant spinocerebellar ataxia with sensory axonal
            neuropathy (SCA4): clinical description and genetic localization to
            chromosome 16q22.1
  JOURNAL   Am J Hum Genet 59 (2), 392-399 (1996)
   PUBMED   8755926
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB461411.1, BC063501.1 and
            BC054486.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_001129729.2.
            
            Summary: The protein encoded by this gene can function as a guanine
            nucleotide exchange factor (GEF) and may play a role in
            intracellular signaling and cytoskeleton dynamics at the Golgi
            apparatus. Polymorphisms in the region of this gene have been found
            to be associated with spinocerebellar ataxia in some study
            populations. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Jan 2015].
            
            Transcript Variant: This variant (4) differs in the 5' UTR compared
            to variant 2. Variants 2, 3, and 4 encode isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC063501.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968832, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000379344.8/ ENSP00000368649.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-54                DB461411.1         9-62
            55-4370             BC063501.1         1-4316
            4371-4512           BC054486.1         4320-4461
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q22.1"
     gene            1..4512
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /note="pleckstrin homology and RhoGEF domain containing
                     G4"
                     /db_xref="GeneID:25894"
                     /db_xref="HGNC:HGNC:24501"
                     /db_xref="MIM:609526"
     exon            1..95
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /inference="alignment:Splign:2.1.0"
     exon            96..763
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /inference="alignment:Splign:2.1.0"
     CDS             265..3840
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /note="isoform 1 is encoded by transcript variant 4;
                     Purkinje cell atrophy associated protein 1; puratrophin-1;
                     PH domain-containing family G member 4; pleckstrin
                     homology domain containing, family G (with RhoGef domain)
                     member 4"
                     /codon_start=1
                     /product="puratrophin-1 isoform 1"
                     /protein_id="NP_001123201.1"
                     /db_xref="CCDS:CCDS32466.1"
                     /db_xref="GeneID:25894"
                     /db_xref="HGNC:HGNC:24501"
                     /db_xref="MIM:609526"
                     /translation="MERPLENGDESPDSQGHATDWRFAVCSFRDAWEEEEPASQMHVK
                     DPGPPRPPAGATQDEELQGSPLSRKFQLPPAADESGDAQRGTVESSSVLSEGPGPSGV
                     ESLLCPMSSHLSLAQGESDTPGVGLVGDPGPSRAMPSGLSPGALDSDPVGLGDPLSEI
                     SKLLEAAPSGSGLPKPADCLLAQDLCWELLASGMATLPGTRDVQGRAVLLLCAHSPAW
                     LQSECSSQELIRLLLYLRSIPRPEVQALGLTVLVDARICAPSSSLFSGLSQLQEAAPG
                     AVYQVLLVGSTLLKEVPSGLQLEQLPSQSLLTHIPTAGLPTSLGGGLPYCHQAWLDFR
                     RRLEALLQNCQAACALLQGAIESVKAVPQPMEPGEVGQLLQQTEVLMQQVLDSPWLAW
                     LQCQGGRELTWLKQEVPEVTLSPDYRTAMDKADELYDRVDGLLHQLTLQSNQRIQALE
                     LVQTLEARESGLHQIEVWLQQVGWPALEEAGEPSLDMLLQAQGSFQELYQVAQEQVRQ
                     GEKFLQPLTGWEAAELDPPGARFLALRAQLTEFSRALAQRCQRLADAERLFQLFREAL
                     TWAEEGQRVLAELEQERPGVVLQQLQLHWTRHPDLPPAHFRKMWALATGLGSEAIRQE
                     CRWAWARCQDTWLALDQKLEASLKLPPVGSTASLCVSQVPAAPAHPPLRKAYSFDRNL
                     GQSLSEPACHCHHAATIAACRRPEAGGGALPQASPTVPPPGSSDPRSLNRLQLVLAEM
                     VATEREYVRALEYTMENYFPELDRPDVPQGLRGQRAHLFGNLEKLRDFHCHFFLRELE
                     ACTRHPPRVAYAFLRHRVQFGMYALYSKNKPRSDALMSSYGHTFFKDKQQALGDHLDL
                     ASYLLKPIQRMGKYALLLQELARACGGPTQELSALREAQSLVHFQLRHGNDLLAMDAI
                     QGCDVNLKEQGQLVRQDEFVVRTGRHKSVRRIFLFEELLLFSKPRHGPTGVDTFAYKR
                     SFKMADLGLTECCGNSNLRFEIWFRRRKARDTFVLQASSLAIKQAWTADISHLLWRQA
                     VHNKEVRMAEMVSMGVGNKAFRDIAPSEEAINDRTVNYVLKCREVRSRASIAVAPFDH
                     DSLYLGASNSLPGDPASCSVLGSLNLHLYRDPALLGLRCPLYPSFPEEAALEAEAELG
                     GQPSLTAEDSEISSQCPSASGSSGSDSSCVSGQALGRGLEDLPCV"
     exon            764..859
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /inference="alignment:Splign:2.1.0"
     exon            860..983
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /inference="alignment:Splign:2.1.0"
     exon            984..1077
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /inference="alignment:Splign:2.1.0"
     exon            1078..1155
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..1269
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /inference="alignment:Splign:2.1.0"
     exon            1270..1368
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /inference="alignment:Splign:2.1.0"
     exon            1369..1517
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /inference="alignment:Splign:2.1.0"
     exon            1518..1656
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /inference="alignment:Splign:2.1.0"
     exon            1657..1773
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /inference="alignment:Splign:2.1.0"
     exon            1774..1956
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /inference="alignment:Splign:2.1.0"
     exon            1957..2459
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /inference="alignment:Splign:2.1.0"
     exon            2460..2706
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /inference="alignment:Splign:2.1.0"
     exon            2707..2796
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /inference="alignment:Splign:2.1.0"
     exon            2797..3018
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /inference="alignment:Splign:2.1.0"
     exon            3019..3189
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /inference="alignment:Splign:2.1.0"
     exon            3190..3367
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /inference="alignment:Splign:2.1.0"
     exon            3368..3484
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /inference="alignment:Splign:2.1.0"
     exon            3485..3718
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /inference="alignment:Splign:2.1.0"
     exon            3719..3834
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /inference="alignment:Splign:2.1.0"
     exon            3835..4512
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4389..4394
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /note="hexamer: AATAGA"
     polyA_site      4408
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
     regulatory      4490..4495
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /note="hexamer: AATAAA"
     polyA_site      4512
                     /gene="PLEKHG4"
                     /gene_synonym="ARHGEF44; PRTPHN1; SCA4"
                     /note="major polyA site"
ORIGIN      
        1 gtgacgccgc gcggccgcgc tgtagtggcc gtcgaggccg ccgtctgttc gaaggctgtc
       61 cgacttcacg gttccccgcg gctcggcccg ggggcgcctg aattgcagtt cctgtgccct
      121 ggcactaaga ctggcacctc ctgcggccca tgcccttcgc ctgcattgcc cactgagtcc
      181 cactcgactg tcttcagcgc ggttcacact gagacccagc cctctcccgc tggccccgag
      241 caggcccacc tttaggaggg cgtgatggaa aggcccctgg agaatgggga tgagtcccca
      301 gactctcagg gccatgccac cgactggaga tttgctgtgt gcagtttcag ggatgcctgg
      361 gaagaggagg aacctgcttc ccagatgcac gttaaggacc caggtcctcc aagaccacca
      421 gccggggcca cccaggatga ggagctacag ggcagcccct tgtccaggaa attccagtta
      481 cccccagctg cagatgagtc gggggatgcc cagaggggca cagtagaaag ctcctcagtc
      541 ctgtcagaag ggccaggccc ctctggagtg gagagtctcc tatgccccat gtcctcccac
      601 ctcagcttgg cacagggtga gagtgacacc ccaggggtag ggttggtagg ggacccaggt
      661 ccaagcaggg cgatgccatc tggcttgagc cctggggcat tggacagcga ccctgtgggc
      721 cttggagacc ctttatcaga aatatcaaag ctgctggagg cagcccccag tggatccggc
      781 ctccctaagc ctgctgactg cctcctggcc caagacctct gttgggagct gctggccagt
      841 ggtatggcca ccttgccagg gactcgggat gtccaaggcc gggcagtgct gcttctgtgt
      901 gcccacagcc cagcctggct tcagtctgag tgcagcagcc aggaactcat ccgcctcctg
      961 ctgtacctgc gaagcatccc caggcccgaa gtacaggcac tgggactgac agtgctagtt
     1021 gatgcccgaa tttgtgctcc aagttcttcc ctcttctctg ggctcagcca actacaagaa
     1081 gcagccccag gggccgtgta ccaggtgctg ctagtgggaa gcacgctgct gaaggaagtg
     1141 ccttccgggc tgcagctgga gcagttgcct tctcagagcc tgctgaccca catcccaacg
     1201 gcggggctgc ccacttcgct aggaggaggc ctgccttact gccaccaggc ctggctggat
     1261 ttccgaaggc ggctggaagc tctactacag aactgccagg cagcttgtgc cctgctccag
     1321 ggggccatcg aaagtgtgaa ggctgtgccc cagcccatgg agcctgggga ggtcggtcag
     1381 ctgctacagc agacagaggt cctgatgcag caggtgctag actcgccatg gctggcatgg
     1441 ctacaatgcc aggggggccg ggagctgaca tggctgaagc aagaggtccc agaggtgacc
     1501 ctgagcccag actacaggac ggcaatggac aaggctgacg agctatatga ccgggtggat
     1561 ggattgctgc accaactgac cctgcagagc aaccagcgaa tacaggccct agagttggtc
     1621 caaacactgg aggcccggga aagcggactg caccagattg aagtgtggct gcagcaggtg
     1681 ggctggccag cactggagga ggctggggag ccctcgctgg acatgctgct ccaggcccaa
     1741 ggctcttttc aggagctgta ccaggttgcc caggagcagg tcaggcaagg ggagaagttt
     1801 ctgcagccgc tgactggctg ggaggcggct gaactggacc cccctggggc acgctttctg
     1861 gccctgcgag cccagctgac tgaattctct agggctttgg cccagcggtg ccagcggctg
     1921 gcggatgctg agaggctgtt tcagctcttc agggaggcct tgacgtgggc tgaggagggg
     1981 cagcgagtgt tggcagagct ggagcaggaa cgcccggggg ttgtgttgca gcagctgcag
     2041 ctgcactgga ccaggcaccc tgacttgcct cctgcccact tccgaaagat gtgggctctg
     2101 gccacggggc tgggctcaga ggccatccgc caggagtgcc gctgggcctg ggcgcggtgc
     2161 caggacacct ggctggccct ggaccaaaag cttgaggctt cactgaagct accaccggtg
     2221 ggcagcacag ctagcctgtg tgtcagccag gtccccgctg cacctgccca ccctcccctg
     2281 aggaaggcct acagcttcga tcggaatctg gggcagagtc tcagtgaacc tgcctgccac
     2341 tgccaccatg cggccactat tgctgcctgc cgcagaccag aggctggagg aggtgccctg
     2401 ccccaggcat cccctactgt gcctccacca ggcagctctg accccaggag cctcaacagg
     2461 ctacagctgg tgctggcaga gatggtggcc acggagcggg agtatgtccg ggctctagag
     2521 tacactatgg agaactattt ccccgagctg gatcgccccg atgtgcccca gggcctccgc
     2581 ggtcagcgtg cccacctctt tggcaacctg gagaagctgc gggacttcca ctgccacttc
     2641 ttcctgcgtg agctggaggc ttgcacccgg cacccaccac gagtggccta tgccttcctg
     2701 cgccataggg tgcagtttgg gatgtacgcg ctctacagca agaataagcc tcgctccgat
     2761 gccctgatgt caagctatgg gcacaccttc ttcaaggaca agcagcaagc actgggggac
     2821 cacctggacc tggcctccta cctgctaaag cccatccagc gcatgggcaa gtacgcactg
     2881 ctgctgcagg agctggcacg ggcctgcggg ggccccacgc aggagctcag tgcgctgcgg
     2941 gaggcccaga gccttgtgca cttccagctg cggcacggaa acgacctgct ggccatggac
     3001 gccatccagg gctgtgatgt taacctcaag gaacaggggc agctggtgcg acaggatgag
     3061 tttgtggtgc gcactgggcg ccacaagtcc gtgcgccgca tcttcctttt tgaggagctg
     3121 ctgctcttca gcaagcctcg ccatgggccc acaggggttg acacatttgc ctacaagcgc
     3181 tccttcaaga tggcagacct tggtctcact gagtgctgtg ggaacagcaa cctgcgcttc
     3241 gagatctggt tccgccgccg caaggccagg gacacctttg tgctgcaggc ctccagcctg
     3301 gctatcaagc aggcctggac agctgacatc tcccacctgc tttggaggca ggccgtccac
     3361 aacaaggagg tgcgcatggc tgagatggtg tccatgggtg tggggaacaa ggccttccga
     3421 gacattgctc ccagcgagga agccatcaac gaccgcaccg tcaactatgt cctgaagtgc
     3481 cgagaagttc gctctcgggc gtccattgcc gtagccccgt ttgaccatga cagcctctac
     3541 ctgggggcct cgaactccct tcctggagac cctgcctctt gctctgttct ggggtccctc
     3601 aacctgcacc tgtacagaga cccagctctt ctgggtctcc gctgtcccct gtatcccagc
     3661 ttcccagagg aagcagcact ggaggctgag gcagagctgg gcggccagcc ctctttgact
     3721 gctgaggact cagagatctc gtcccaatgc ccatcagcca gtggctccag tggctctgac
     3781 agcagctgtg tgtcagggca ggccctgggt aggggcctgg aggacttacc ctgtgtctga
     3841 gcccgggact ggacgagcag tagatccagc agcctgcagc tccaaggaac attgcctctc
     3901 tggatctgct gtgaccaggg tgtggctgac acctgggcta cctccaacct acatgtgcaa
     3961 cgctgttgac taccctttct gatgtgtgtg gccattggac taactggcac ggggcctctc
     4021 tagggaagtc tggttgtaga gcctgaatag gctcctggcc ccatgacccc ttctcctgtc
     4081 cccagctccc atcccagttg tgggttaaga ataggctaga gcagacattg ggtgtttcca
     4141 tgctgtaggc tggtggggga ccatgtgcct ctaggcagtg actagggtgc ccccacccct
     4201 caggaagaac acaggtgggc tcctagcagc tgatccccaa tgcctggcct taaagccgag
     4261 ctcagttacc atagggacag gtccacctct actgggccct catgcttgcc tttcctggcc
     4321 cccaggccca gccccttttt actggggcag tttcgttatt ttgacttgat gccttttgaa
     4381 taactttcaa tagaattgtc taaaattatc ttactggttg ttaggccttt ggtgtctcag
     4441 agaaggagtc taggtctttg atgtgtgatt taatctttta tttgtttata ataaaaaata
     4501 gactgatatg ta
//
