LOCUS       NM_022835               8048 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens pleckstrin homology and RhoGEF domain containing G2
            (PLEKHG2), transcript variant 1, mRNA.
ACCESSION   NM_022835 XM_290828
VERSION     NM_022835.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 8048)
  AUTHORS   Nishikawa,M., Sato,K., Nakano,S., Yamakawa,H., Nagase,T. and
            Ueda,H.
  TITLE     Specific activation of PLEKHG2-induced serum response
            element-dependent gene transcription by four-and-a-half LIM domains
            (FHL) 1, but not FHL2 or FHL3
  JOURNAL   Small GTPases 10 (5), 361-366 (2019)
   PUBMED   28489964
  REMARK    GeneRIF: results suggest that FHL1A specifically interacts with
            PLEKHG2 to regulate a function of PLEKHG2 that is modified by the
            interaction of Gbetagamma and Galphas.
REFERENCE   3  (bases 1 to 8048)
  AUTHORS   Sugiyama,K., Tago,K., Matsushita,S., Nishikawa,M., Sato,K.,
            Muto,Y., Nagase,T. and Ueda,H.
  TITLE     Heterotrimeric G protein Galphas subunit attenuates PLEKHG2, a Rho
            family-specific guanine nucleotide exchange factor, by direct
            interaction
  JOURNAL   Cell Signal 32, 115-123 (2017)
   PUBMED   28108261
  REMARK    GeneRIF: This is the first report to show that PLEKHG2 is a novel
            effector of Galphas, and is negatively regulated by the Galphas
            subunit through direct interaction.
REFERENCE   4  (bases 1 to 8048)
  AUTHORS   Sato,K., Kimura,M., Sugiyama,K., Nishikawa,M., Okano,Y.,
            Nagaoka,H., Nagase,T., Kitade,Y. and Ueda,H.
  TITLE     Four-and-a-half LIM Domains 1 (FHL1) Protein Interacts with the Rho
            Guanine Nucleotide Exchange Factor PLEKHG2/FLJ00018 and Regulates
            Cell Morphogenesis
  JOURNAL   J Biol Chem 291 (48), 25227-25238 (2016)
   PUBMED   27765816
  REMARK    GeneRIF: results provide evidence that FHL1A interacts with PLEKHG2
            and regulates cell morphological change through the activity of
            PLEKHG2.
REFERENCE   5  (bases 1 to 8048)
  AUTHORS   Edvardson,S., Wang,H., Dor,T., Atawneh,O., Yaacov,B., Gartner,J.,
            Cinnamon,Y., Chen,S. and Elpeleg,O.
  TITLE     Microcephaly-dystonia due to mutated PLEKHG2 with impaired actin
            polymerization
  JOURNAL   Neurogenetics 17 (1), 25-30 (2016)
   PUBMED   26573021
  REMARK    GeneRIF: Role of PLEKHG2 in actin polymerization and clinical and
            radiological findings in PLEKHG2 deficiency.
REFERENCE   6  (bases 1 to 8048)
  AUTHORS   Ueda,H., Nagae,R., Kozawa,M., Morishita,R., Kimura,S., Nagase,T.,
            Ohara,O., Yoshida,S. and Asano,T.
  TITLE     Heterotrimeric G protein betagamma subunits stimulate FLJ00018, a
            guanine nucleotide exchange factor for Rac1 and Cdc42
  JOURNAL   J Biol Chem 283 (4), 1946-1953 (2008)
   PUBMED   18045877
  REMARK    GeneRIF: signaling pathway by which G(i)-coupled receptor
            specifically induces Rac and Cdc42 activation through direct
            interaction of Gbetagamma with FLJ00018.
REFERENCE   7  (bases 1 to 8048)
  AUTHORS   Rabizadeh,S. and Bredesen,D.E.
  TITLE     Ten years on: mediation of cell death by the common neurotrophin
            receptor p75(NTR)
  JOURNAL   Cytokine Growth Factor Rev 14 (3-4), 225-239 (2003)
   PUBMED   12787561
  REMARK    Review article
REFERENCE   8  (bases 1 to 8048)
  AUTHORS   Salehi,A.H., Xanthoudakis,S. and Barker,P.A.
  TITLE     NRAGE, a p75 neurotrophin receptor-interacting protein, induces
            caspase activation and cell death through a JNK-dependent
            mitochondrial pathway
  JOURNAL   J Biol Chem 277 (50), 48043-48050 (2002)
   PUBMED   12376548
REFERENCE   9  (bases 1 to 8048)
  AUTHORS   Himmel,K.L., Bi,F., Shen,H., Jenkins,N.A., Copeland,N.G., Zheng,Y.
            and Largaespada,D.A.
  TITLE     Activation of clg, a novel dbl family guanine nucleotide exchange
            factor gene, by proviral insertion at evi24, a common integration
            site in B cell and myeloid leukemias
  JOURNAL   J Biol Chem 277 (16), 13463-13472 (2002)
   PUBMED   11839748
  REMARK    GeneRIF: Activation of clg, a novel dbl family guanine nucleotide
            exchange factor gene, by proviral insertion at evi24, a common
            integration site in B cell and myeloid leukemias
REFERENCE   10 (bases 1 to 8048)
  AUTHORS   Harrington,A.W., Kim,J.Y. and Yoon,S.O.
  TITLE     Activation of Rac GTPase by p75 is necessary for c-jun N-terminal
            kinase-mediated apoptosis
  JOURNAL   J Neurosci 22 (1), 156-166 (2002)
   PUBMED   11756498
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC011500.7, AK024429.1 and
            AK095957.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_022835.2.
            
            Summary: The protein encoded by this gene is a RhoGTPase that can
            activate CDC42 by promoting exchange of GDP for GTP on CDC42. The
            encoded protein is activated by binding to the beta and gamma
            subunits of heterotrimeric guanine nucleotide-binding protein.
            Defects in this gene have been associated with leukodystrophy and
            acquired microcephaly with or without dystonia. [provided by
            RefSeq, May 2017].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK024429.1, SRR7346977.2690117.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000425673.6/ ENSP00000392906.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-441               AC011500.7         17493-17933
            442-3740            AK024429.1         1-3299
            3741-7845           AC011500.7         28932-33036
            7846-8048           AK095957.1         2280-2482
FEATURES             Location/Qualifiers
     source          1..8048
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.2"
     gene            1..8048
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
                     /note="pleckstrin homology and RhoGEF domain containing
                     G2"
                     /db_xref="GeneID:64857"
                     /db_xref="HGNC:HGNC:29515"
                     /db_xref="MIM:611893"
     exon            1..744
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
                     /inference="alignment:Splign:2.1.0"
     exon            745..875
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
                     /inference="alignment:Splign:2.1.0"
     CDS             767..4927
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
                     /note="isoform 1 is encoded by transcript variant 1;
                     common-site lymphoma/leukemia guanine nucleotide exchange
                     factor; pleckstrin homology domain-containing family G
                     member 2; PH domain-containing family G member 2;
                     pleckstrin homology domain containing, family G (with
                     RhoGef domain) member 2"
                     /codon_start=1
                     /product="pleckstrin homology domain-containing family G
                     member 2 isoform 1"
                     /protein_id="NP_073746.2"
                     /db_xref="CCDS:CCDS33022.2"
                     /db_xref="GeneID:64857"
                     /db_xref="HGNC:HGNC:29515"
                     /db_xref="MIM:611893"
                     /translation="MPEGAQGLSLSKPSPSLGCGRRGEVCDCGTVCETRTAPAAPTMA
                     SPRGSGSSTSLSTVGSEGDPAPGPTPACSASRPEPLPGPPIRLHLSPVGIPGSARPSR
                     LERVAREIVETERAYVRDLRSIVEDYLGPLLDGGVLGLSVEQVGTLFANIEDIYEFSS
                     ELLEDLENSSSAGGIAECFVQRSEDFDIYTLYCMNYPSSLALLRELSLSPPAALWLQE
                     RQAQLRHSLPLQSFLLKPVQRILKYHLLLQELGKHWAEGPGTGGREMVEEAIVSMTAV
                     AWYINDMKRKQEHAARLQEVQRRLGGWTGPELSAFGELVLEGAFRGGGGGGPRLRGGE
                     RLLFLFSRMLLVAKRRGLEYTYKGHIFCCNLSVSESPRDPLGFKVSDLTIPKHRHLLQ
                     AKNQEEKRLWIHCLQRLFFENHPASIPAKAKQVLLENSLHCAPKSKPVLEPLTPPLGS
                     PRPRDARSFTPGRRNTAPSPGPSVIRRGRRQSEPVKDPYVMFPQNAKPGFKHAGSEGE
                     LYPPESQPPVSGSAPPEDLEDAGPPTLDPSGTSITEEILELLNQRGLRDPGPSTHDIP
                     KFPGDSQVPGDSETLTFQALPSRDSSEEEEEEEEGLEMDERGPSPLHVLEGLESSIAA
                     EMPSIPCLTKIPDVPNLPEIPSRCEIPEGSRLPSLSDISDVFEMPCLPAIPSVPNTPS
                     LSSTPTLSCDSWLQGPLQEPAEAPATRRELFSGSNPGKLGEPPSGGKAGPEEDEEGVS
                     FTDFQPQDVTQHQGFPDELAFRSCSEIRSAWQALEQGQLARPGFPEPLLILEDSDLGG
                     DSGSGKAGAPSSERTASRVRELARLYSERIQQMQRAETRASANAPRRRPRVLAQPQPS
                     PCLPQEQAEPGLLPAFGHVLVCELAFPLTCAQESVPLGPAVWVQAAIPLSKQGGSPDG
                     QGLHVSNLPKQDLPGIHVSAATLLPEQGGSRHVQAPAATPLPKQEGPLHLQVPALTTF
                     SDQGHPEIQVPATTPLPEHRSHMVIPAPSTAFCPEQGHCADIHVPTTPALPKEICSDF
                     TVSVTTPVPKQEGHLDSESPTNIPLTKQGGSRDVQGPDPVCSQPIQPLSWHGSSLDPQ
                     GPGDTLPPLPCHLPDLQIPGTSPLPAHGSHLDHRIPANAPLSLSQELPDTQVPATTPL
                     PLPQVLTDIWVQALPTSPKQGSLPDIQGPAAAPPLPEPSLTDTQVQKLTPSLEQKSLI
                     DAHVPAATPLPERGGSLDIQGLSPTPVQTTMVLSKPGGSLASHVARLESSDLTPPHSP
                     PPSSRQLLGPNAAALSRYLAASYISQSLARRQGPGGGAPAASRGSWSSAPTSRASSPP
                     PQPQPPPPPARRLSYATTVNIHVGGGGRLRPAKAQVRLNHPALLASTQESMGLHRAQG
                     APDAPFHM"
     exon            876..1144
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
                     /inference="alignment:Splign:2.1.0"
     exon            1145..1245
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
                     /inference="alignment:Splign:2.1.0"
     exon            1246..1312
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
                     /inference="alignment:Splign:2.1.0"
     exon            1313..1359
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
                     /inference="alignment:Splign:2.1.0"
     exon            1360..1510
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
                     /inference="alignment:Splign:2.1.0"
     exon            1511..1648
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
                     /inference="alignment:Splign:2.1.0"
     exon            1649..1849
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
                     /inference="alignment:Splign:2.1.0"
     exon            1850..1942
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
                     /inference="alignment:Splign:2.1.0"
     exon            1943..2029
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
                     /inference="alignment:Splign:2.1.0"
     exon            2030..2063
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
                     /inference="alignment:Splign:2.1.0"
     exon            2064..2165
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
                     /inference="alignment:Splign:2.1.0"
     exon            2166..2213
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
                     /inference="alignment:Splign:2.1.0"
     exon            2214..2252
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
                     /inference="alignment:Splign:2.1.0"
     exon            2253..2269
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
                     /inference="alignment:Splign:2.1.0"
     exon            2270..2443
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
                     /inference="alignment:Splign:2.1.0"
     exon            2444..3365
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
                     /inference="alignment:Splign:2.1.0"
     exon            3366..8048
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      5149
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
                     /note="major polyA site"
     polyA_site      5293
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
                     /note="major polyA site"
     regulatory      7849..7854
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
                     /note="hexamer: AATGAA"
     polyA_site      7867
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
     regulatory      8020..8025
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
                     /note="hexamer: AATAAA"
     polyA_site      8048
                     /gene="PLEKHG2"
                     /gene_synonym="ARHGEF42; CLG; CTB-60E11.4; LDAMD"
ORIGIN      
        1 agatgtggcg ccgccgccgt cacacgagcc ccgtgacacc cagccgggag cccgaggggt
       61 ccagaggctc ggacccgggc gtccggaatt tctgcaccgc atcctgagag accccgaggt
      121 ccagaccccg gcgcccaggc tggaggactt gagccatcca ggtgcgcagc gccccctcct
      181 ggagcagccg ggagcccgga cgtcaccccg cgagaccctg gaggccgagc gccccgtatc
      241 cccgtttcaa gaactcagga tccaggaccg cagactccct ccagggctcc gatcccaaga
      301 ccccgcgttg gaggaacttg agatccggac ttctaagcgc cccaaccagt ttcgggctgc
      361 agcggccatc gcttcaaggg gacccaggac tttgggaccc ccctgtgctc ccacctggag
      421 ggagtttcat tcctgcccct agaattaggg gaactcagaa atccatcccc caccccacgg
      481 ccctacctag aagacttagg aatgtgggtc cccagcgccg ccatctgaga ctccagagtc
      541 gaggctccta gcatccccta cgtagaacac tgagaaattc cgactgcggg accccagtct
      601 ccgagagacc ccagattcta ttcctggagc ctgagagccc gaagttcacg cccctgagtc
      661 tgggctccgc acctccctgg ggaccgcgtc ggggtcgcgc aggagccggg ctgcctcgag
      721 ccggggctgg aggctccccg ggaggactct gctgggccgc tcagccatgc ctgagggagc
      781 ccaaggactg agcctctcca aacctagccc aagcctcggg tgtggccgaa gaggtgaagt
      841 gtgtgactgt ggcaccgtgt gtgagactcg gacagctcct gcagccccca ccatggcctc
      901 cccccgaggt tctgggagct ccacatccct gagcacagtg ggctctgagg gggatccagc
      961 ccctgggccc actccagcct gctcagcctc caggccagag ccccttccag ggcctcccat
     1021 ccgcctacat ctctctccgg tggggatccc aggttcagcc agaccctcaa ggctggagcg
     1081 tgtggcccgg gagatcgtgg agacagaacg ggcctatgtc agggacctcc gcagcatcgt
     1141 ggaggactac ctgggccctc tgctggacgg cggggtcctg gggctgagcg tggagcaggt
     1201 gggcacgctg tttgccaaca ttgaggacat ctacgagttc agcagcgagc tcctggagga
     1261 cttggagaac agcagcagcg ccgggggtat tgccgagtgc ttcgtgcaga ggagcgagga
     1321 ttttgacatc tacacattgt actgcatgaa ctacccgagc tccctggccc tgctccggga
     1381 gctgtcgttg tctccgccag cagccctgtg gctgcaggag cgccaggccc agcttcgcca
     1441 ctcgctgccc ctgcagagct tcctgctgaa acctgtccag cgcattctca agtaccatct
     1501 gctgctgcag gaactaggga agcactgggc ggagggccca ggcactgggg gtcgcgagat
     1561 ggtggaggaa gctattgtgt ccatgacagc ggttgcctgg tacatcaacg acatgaagcg
     1621 caagcaggag catgcagcgc gcctccagga agtgcagcgg cggctgggtg gctggaccgg
     1681 accagagctc agtgcttttg gggaactggt gttggagggc gcgttccgag gaggcggagg
     1741 gggtggcccc cggctacgag ggggtgagcg gctgctcttc ctgttctctc ggatgctgct
     1801 ggtggccaag cgcagggggc tggagtacac ctacaaaggc cacatcttct gctgcaacct
     1861 gagcgtgagc gagagtcccc gagaccctct agggttcaag gtgtctgatc tgaccattcc
     1921 caagcacaga cacctgctcc aggccaagaa ccaagaagag aagaggctgt ggattcactg
     1981 tctccagcgc ctcttctttg agaaccaccc tgcctccatc cctgccaagg caaagcaagt
     2041 tctccttgaa aacagcctgc attgtgcccc caaaagtaag cctgtcctag agcccctgac
     2101 acccccactt gggtctcctc gacctcgaga tgctagaagt tttacccctg ggcgaaggaa
     2161 cacagctcca tctcctgggc cctctgtgat tcgccgaggc cgcaggcagt ctgagccggt
     2221 gaaggaccct tatgtcatgt tcccacagaa cgctaagcct ggattcaagc acgctggcag
     2281 cgaaggggaa ctctaccctc cagaatctca gccaccagtt tcaggctctg caccccctga
     2341 ggacctggag gatgctggac ccccaacact ggacccctct gggacctcaa tcactgaaga
     2401 aatcctggaa ctgctgaatc agcgaggcct tcgagatcca gggccgtcca cccatgacat
     2461 tcccaagttc cccggagact cccaggtgcc tggcgacagc gaaaccctca cattccaagc
     2521 cctgcccagc cgggactctt cagaagagga ggaggaggaa gaggaagggc tggagatgga
     2581 tgaacggggg ccttccccac tccacgtcct ggaagggctc gaaagttcca ttgcagctga
     2641 aatgcccagc attccctgcc ttaccaaaat tcctgacgtg cccaaccttc ctgaaattcc
     2701 cagccgctgt gaaattcccg aaggttctcg ccttcctagt ctctctgaca tttccgatgt
     2761 ttttgagatg ccctgccttc cagccatacc tagtgtcccc aacaccccca gtctgtctag
     2821 cactcccacc ctctcctgtg actcctggct ccaagggcct ctgcaggaac cagctgaggc
     2881 tccagccacc aggagagaac tgttttctgg gagcaatcct gggaaactgg gagagccgcc
     2941 ttcaggaggc aaggcagggc cagaggagga tgaagaaggg gtatcattca cagacttcca
     3001 gccccaggat gtcacccaac atcagggatt cccagatgag ctggcattcc gctcttgctc
     3061 agaaatccgg agcgcctggc aggcattgga acagggacag ctggcccggc caggcttccc
     3121 agagccactg ctgatcctgg aggattcgga tctgggtgga gacagcggga gcgggaaggc
     3181 aggagccccg agttcagaaa ggacggcgtc ccgagtgcga gagctggccc ggctttacag
     3241 cgagcggatc cagcagatgc agcgggcgga gactcgggca tcagccaatg ccccgcgccg
     3301 ccggcctcgg gttctggccc aaccccagcc atccccctgt ctgccccagg agcaggcaga
     3361 gccagggctc ctgcctgcct ttggacacgt gctggtatgt gagctggcct tcccactgac
     3421 atgtgcccag gagtctgtcc ccctgggtcc tgctgtctgg gttcaagctg ccataccttt
     3481 gtcaaagcag ggaggcagcc cggatggcca gggtctacat gtttccaatt tgcctaagca
     3541 agaccttccg ggcatccacg tttcagctgc tacccttttg cctgagcaag gaggttcccg
     3601 gcatgtccag gctccagccg ccacaccttt gcccaagcaa gaaggccccc tgcacctcca
     3661 ggtgccggct cttacaactt tctctgatca aggccaccca gaaatccaag ttccagccac
     3721 cactcctttg cctgagcata gaagtcacat ggttatacca gctccatcca ccgccttttg
     3781 tcctgagcag ggacactgtg cggacatcca cgttcccacc actccagctt tgcccaagga
     3841 gatttgttct gatttcacag tttcagtcac cacccctgtg cccaagcaag aaggtcacct
     3901 agacagcgag agcccaacca atatcccact gacaaagcaa ggaggttcca gggatgttca
     3961 gggcccagac cctgtctgca gtcaacccat ccagcctttg tcttggcatg gaagcagcct
     4021 ggatccccag ggcccaggcg acaccctacc acccttgcca tgtcacctcc cagaccttca
     4081 gattccaggt acctcacctt tgcctgcaca tggaagccac ctggaccatc ggatcccagc
     4141 caacgcccca ctgtctttgt cccaggagct cccagacact caggttccag ctaccacacc
     4201 tttgcccctg ccacaagtcc tcacagacat ctgggtccaa gccctcccaa cttcacccaa
     4261 gcagggaagc ctcccagaca tccagggtcc agcggctgca cctccacttc cggagccaag
     4321 ccttacagat acacaggtcc aaaaactcac accttcgttg gagcagaaga gcctcataga
     4381 tgcccatgtt ccagctgcca cacctttacc tgagagagga ggctctctag acattcaggg
     4441 cctctcaccc accccagttc agaccaccat ggttttgtcc aaaccaggag gctccttagc
     4501 ctctcacgtt gccaggttgg agtcttcaga cttgacgcca cctcatagtc ccccaccttc
     4561 cagccgtcag ctcctgggcc ccaatgcagc tgccctctcc agatacctgg cagcctcata
     4621 tatcagccag agcctggctc ggcggcaggg gcctggggga ggggcccccg cagcctcccg
     4681 gggctcctgg tcctctgctc ccacgtcacg ggcatcttcg ccgccccccc agccccagcc
     4741 accacctccc ccagccaggc ggctcagcta tgccacgacg gttaacatcc acgtgggcgg
     4801 gggtgggcgg ctgcggccag ccaaggccca ggtccggttg aaccaccctg ctctcttggc
     4861 ctccacacag gaatctatgg gccttcacag ggcccagggg gctcctgatg cccccttcca
     4921 catgtgagcc aggacatgag gcttccctga agcaaggatt tcagccagat gccatagacc
     4981 ctcagaactt gacctggaag tccagacact gaacgcaggc ctcaaaactg ctgcggcctt
     5041 ccaactcctg gtatctgcat cggcgaatgg cccttcttgc cttgatccac agggatgggg
     5101 aagggaggaa tgtcattaat gttttgttaa tactgattct ttcatgcaat gatgtgtatt
     5161 ttcccattct ggaggctgtg ggagatgaca agacaatgaa tgggaaggtc tgacacagaa
     5221 caaatcagcg gttctgaaag cttggggaat ctcagactcc tttgagaatt attggaaaat
     5281 ggacccacta taacttggcg tgtgtgtgaa ctgcttgatg cccatccagg aaagccaagt
     5341 taagaagctt tgcttcaagt agacactaga aatccattcc cttggcaatt tatacagttc
     5401 acgtctccca ccatccgttc atctcaccca ccctgccatc tctccaccta tccatctggc
     5461 tattgctcca tctagctttc ccgctccatc tacccatctt ccaatccatc atctcacgta
     5521 tctgccttgc ttatccaact gtctgcctta ttcacccacc catcccttta tcattctaac
     5581 cccatctggc attccatccc tccatttatc catcttccca cttacccatc ttctccatcc
     5641 agcactccgt tcatcagtcc gtctctccct ccatatttcc atctgtccat tcatccactc
     5701 attttgccat ccatacatat tcacccagcc atctcccaat ccatcgacat accccatccc
     5761 tccctcactg gactctccgg ggtcttagta ctatgatact tcagagacaa ttccatgctg
     5821 atacggggga gatcaatttt ggggctctgg gataagtctg tcccacatct gtctgatttc
     5881 ccttggcatc cttcccgtca gttcctcttg aatatcccag ttcttgtacc cactcacata
     5941 cctcagatcc cttcactcct ccccgcagtt tgcagcctct acaattcatc cacatgctcc
     6001 ctcccccagc aaaataattc aggtcgtgga gacaaaaggc ttttatgtta ggttctgtct
     6061 gcccccttgt tccagcagtg gtatgcccag tgagaggcac aatttaaacg gtttctttgg
     6121 tgatgatatt agctactttc atcaaatact tagcctcttg tgctttagca cattatttat
     6181 gtaagctccc ctaggattct ctgaagcacc ctggtgtcct ggctgtgaat tcaagtccca
     6241 tctttactta tttggtgtgg gtcctccctc tgagccggtt ttctcatcta caaaatgaag
     6301 acaacagtac ttccctcctg ggatcattga aagatttagg gagcatatcc ttccacttcc
     6361 caaacatatt tgaacctctg acttgcttag gagcatcatt caaatcacag atcccccagt
     6421 atctagtccc aacctattga atcaggatcc ccagggaagg aggagcttgg gaatctgatt
     6481 tttttttttt tttttagata aagtctcact ctgtcaccca ggcttgagtg cagtggcaaa
     6541 atcttggctc attgcaacct ccacctcctg ggttcaagca attctcatgc ctcagcttcc
     6601 cgacatagct gggattatag gcgtgcacca ccatgcccag ctaatttttc gttgttgttg
     6661 ttgttgtttt gtttttgaga cagagtctcg ctctgttgcc caggctggag tacagtggca
     6721 cgatcttggc tcactgcaag ctccgcttcc cgggttcacg ccattctcct gcctcagcct
     6781 cccgagtagc tgggaccaca ggtgcccgcc accacgcccg gctaattttt ttggtatttt
     6841 tagtagagac ggggttttac catgttagcc aggatggtct cgatctcctg acctcgtgat
     6901 cctcccgcct cggcctccca aagtgctggc attacaggcg tgagccatca ttcctggctg
     6961 ggaatccgaa tttttttttt tttttagacg gagtttcgct cttgttgccc aggctggagt
     7021 tcaatagcat gatctcggct cactgcaacc tctgcctccc aggttcaagt gattctcctg
     7081 cctctagctg ggattgcagg cgcccacgac cacgcccagc taatttttgt attttgtagt
     7141 agagacggga tttcgccata ttggccaggc catagtggtc ttggactcct gacctcaggt
     7201 gatccgccca cctcggcctc ccaaagtgtt gggtttacag gcgtgagcta ccataccctg
     7261 cccaggaatc tgaattttta gcaatcagtt agcagcaaat aagctgagat ttcctgggta
     7321 ctaagcacat actatgagca ttacagccgc tttatataag ctcgttcact gtcctcatta
     7381 cattcctaat atgcaagtga gcatgttttt taatcctcag tctagaggtg aggaaactga
     7441 ggcaggcaca gagaggctaa gggatttgcc tagtcgcaca gctaggaagg tgcagcactg
     7501 agactcgaat ccacacagct tggcttaagt ctaccattaa atttgcctgt cctggccagg
     7561 tgcggtggct cacgcctgta atcccaacac ttcgtgaggc caaggcaggt ggatcacttg
     7621 aagtgaggag ttcgagacca caccagcctg gccaacatgg tgaaatcccg tctttactaa
     7681 aaatacaaaa aattagctgg gcgtggtggt gggtgccggt aatcctagct actcaggagg
     7741 ctgaggcagg agaatcactt gaacctggga ggtggaggtt gcagtgaacc gagattgcgc
     7801 cactagactc cagcctggga gacgagcaaa actgtctcaa aaaaataaaa tgaaatacat
     7861 aaataaatgt gcctatcctg tgccgggtgc tgttcttgcc agtggggata catgagagaa
     7921 cactgccttg cccgcatgag gcttgtggcc tggtactgtg cacacctcag gggattctga
     7981 taattagctg agcacagttg ccagcacagg gcctggctca ataaatatta gctgctggct
     8041 tctgctca
//
