LOCUS       NM_001309452            3910 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens pleckstrin homology domain containing B2 (PLEKHB2),
            transcript variant 14, mRNA.
ACCESSION   NM_001309452 NR_049792
VERSION     NM_001309452.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Matsudaira,T., Mukai,K., Noguchi,T., Hasegawa,J., Hatta,T.,
            Iemura,S.I., Natsume,T., Miyamura,N., Nishina,H., Nakayama,J.,
            Semba,K., Tomita,T., Murata,S., Arai,H. and Taguchi,T.
  TITLE     Endosomal phosphatidylserine is critical for the YAP signalling
            pathway in proliferating cells
  JOURNAL   Nat Commun 8 (1), 1246 (2017)
   PUBMED   29093443
  REMARK    GeneRIF: Knockdown of evectin-2 (a recycling endosome-resident
            protein) suppresses nuclear localization of YAP and YAP-dependent
            transcription. Evectin-2 knockdown reduces the ubiquitination and
            increased the level of Lats1.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3910)
  AUTHORS   Okazaki,S., Kato,R., Uchida,Y., Taguchi,T., Arai,H. and
            Wakatsuki,S.
  TITLE     Structural basis of the strict phospholipid binding specificity of
            the pleckstrin homology domain of human evectin-2
  JOURNAL   Acta Crystallogr D Biol Crystallogr 68 (Pt 2), 117-123 (2012)
   PUBMED   22281740
  REMARK    GeneRIF: The crystal structure at 1.75 A resolution of an apo form
            of human evectin-2 pleckstrin homology domain, in which the
            ligand-binding site is free from crystal packing and is thus
            appropriate for comparison with the structure of the complex, is
            reported.
REFERENCE   4  (bases 1 to 3910)
  AUTHORS   Uchida,Y., Hasegawa,J., Chinnapen,D., Inoue,T., Okazaki,S.,
            Kato,R., Wakatsuki,S., Misaki,R., Koike,M., Uchiyama,Y., Iemura,S.,
            Natsume,T., Kuwahara,R., Nakagawa,T., Nishikawa,K., Mukai,K.,
            Miyoshi,E., Taniguchi,N., Sheff,D., Lencer,W.I., Taguchi,T. and
            Arai,H.
  TITLE     Intracellular phosphatidylserine is essential for retrograde
            membrane traffic through endosomes
  JOURNAL   Proc Natl Acad Sci U S A 108 (38), 15846-15851 (2011)
   PUBMED   21911378
  REMARK    GeneRIF: Data show that depletion of evt-2 or masking of
            intracellular PS suppressed membrane traffic from REs to the Golgi.
REFERENCE   5  (bases 1 to 3910)
  AUTHORS   Girard,A., Sachidanandam,R., Hannon,G.J. and Carmell,M.A.
  TITLE     A germline-specific class of small RNAs binds mammalian Piwi
            proteins
  JOURNAL   Nature 442 (7099), 199-202 (2006)
   PUBMED   16751776
REFERENCE   6  (bases 1 to 3910)
  AUTHORS   Colland,F., Jacq,X., Trouplin,V., Mougin,C., Groizeleau,C.,
            Hamburger,A., Meil,A., Wojcik,J., Legrain,P. and Gauthier,J.M.
  TITLE     Functional proteomics mapping of a human signaling pathway
  JOURNAL   Genome Res 14 (7), 1324-1332 (2004)
   PUBMED   15231748
REFERENCE   7  (bases 1 to 3910)
  AUTHORS   Lehner,B. and Sanderson,C.M.
  TITLE     A protein interaction framework for human mRNA degradation
  JOURNAL   Genome Res 14 (7), 1315-1323 (2004)
   PUBMED   15231747
REFERENCE   8  (bases 1 to 3910)
  AUTHORS   Dowler,S., Currie,R.A., Campbell,D.G., Deak,M., Kular,G.,
            Downes,C.P. and Alessi,D.R.
  TITLE     Identification of pleckstrin-homology-domain-containing proteins
            with novel phosphoinositide-binding specificities
  JOURNAL   Biochem J 351 (Pt 1), 19-31 (2000)
   PUBMED   11001876
REFERENCE   9  (bases 1 to 3910)
  AUTHORS   Krappa,R., Nguyen,A., Burrola,P., Deretic,D. and Lemke,G.
  TITLE     Evectins: vesicular proteins that carry a pleckstrin homology
            domain and localize to post-Golgi membranes
  JOURNAL   Proc Natl Acad Sci U S A 96 (8), 4633-4638 (1999)
   PUBMED   10200314
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK126289.1, BU174451.1, BC013991.2, BQ719002.1 and AK054998.1.
            
            On Aug 13, 2020 this sequence version replaced NM_001309452.1.
            
            Transcript Variant: This variant (14) contains an alternate exon in
            the 5' UTR, uses a downstream start codon, and lacks an exon in the
            3' coding region, compared to variant 4. The encoded isoform (11)
            is shorter than isoform 4. Both variants 11 and 14 encode the same
            isoform (11).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038196.3343000.1,
                                           SRR14038191.1650631.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                AK126289.1         490-519
            31-790              BU174451.1         1-760
            791-1878            BC013991.2         675-1762
            1879-1985           BQ719002.1         150-256
            1986-3910           AK054998.1         1-1925
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q21.1"
     gene            1..3910
                     /gene="PLEKHB2"
                     /gene_synonym="EVT2"
                     /note="pleckstrin homology domain containing B2"
                     /db_xref="GeneID:55041"
                     /db_xref="HGNC:HGNC:19236"
                     /db_xref="MIM:618452"
     exon            1..63
                     /gene="PLEKHB2"
                     /gene_synonym="EVT2"
                     /inference="alignment:Splign:2.1.0"
     exon            64..108
                     /gene="PLEKHB2"
                     /gene_synonym="EVT2"
                     /inference="alignment:Splign:2.1.0"
     exon            109..206
                     /gene="PLEKHB2"
                     /gene_synonym="EVT2"
                     /inference="alignment:Splign:2.1.0"
     exon            207..359
                     /gene="PLEKHB2"
                     /gene_synonym="EVT2"
                     /inference="alignment:Splign:2.1.0"
     CDS             314..838
                     /gene="PLEKHB2"
                     /gene_synonym="EVT2"
                     /note="isoform 11 is encoded by transcript variant 14;
                     pleckstrin homology domain-containing family B member 2;
                     evectin-2; PH domain-containing family B member 2;
                     pleckstrin homology domain containing, family B (evectins)
                     member 2"
                     /codon_start=1
                     /product="pleckstrin homology domain-containing family B
                     member 2 isoform 11"
                     /protein_id="NP_001296381.1"
                     /db_xref="GeneID:55041"
                     /db_xref="HGNC:HGNC:19236"
                     /db_xref="MIM:618452"
                     /translation="MPMDCINIRTGQECRDTQPPDGKSKDCMLQIVCRDGKTISLCAE
                     STDDCLAWKFTLQDSRTNTAYVGSAVMTDETSVVSSPPPYTAYAAPAPEQAYGYGPYG
                     GAYPPGTQVVYAANGQAYAVPYQYPYAGLYGQQPANQVIIRERYRDNDSDLALGMLAG
                     AATGMALGSLFWVF"
     exon            360..462
                     /gene="PLEKHB2"
                     /gene_synonym="EVT2"
                     /inference="alignment:Splign:2.1.0"
     exon            463..502
                     /gene="PLEKHB2"
                     /gene_synonym="EVT2"
                     /inference="alignment:Splign:2.1.0"
     exon            503..592
                     /gene="PLEKHB2"
                     /gene_synonym="EVT2"
                     /inference="alignment:Splign:2.1.0"
     exon            593..701
                     /gene="PLEKHB2"
                     /gene_synonym="EVT2"
                     /inference="alignment:Splign:2.1.0"
     exon            702..3910
                     /gene="PLEKHB2"
                     /gene_synonym="EVT2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3888..3893
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLEKHB2"
                     /gene_synonym="EVT2"
                     /note="hexamer: ATTAAA"
     polyA_site      3910
                     /gene="PLEKHB2"
                     /gene_synonym="EVT2"
                     /note="major polyA site"
ORIGIN      
        1 agttgcaggc gagcaggcga ggaatcgccg tggcgtcttg gtgttctcca cgctggttcg
       61 caggtgaaga gatggcgttt gtgaagagtg gctggttgct gcgacagagc tggagtgcaa
      121 tggcatgatc tcaacttact gcaacctctg cctcctggct tcaagcaatt ttcctgcttc
      181 agcctcctga gtagctggga ttacaggtac tattttgaag cgctggaaga agaactggtt
      241 tgatctgtgg tcggatggtc acctgatcta ttatgatgac cagactcggc agaatatcga
      301 ggataaggtc cacatgccaa tggactgcat caacatccgc acggggcagg aatgtcggga
      361 tactcagccc ccggatggaa agtcaaaaga ctgcatgctc cagattgttt gtcgagatgg
      421 gaaaacaatt agtctttgtg cagaaagcac agatgattgc ttggcctgga aatttacact
      481 ccaagattct aggacaaaca cagcgtatgt gggctctgca gtcatgaccg atgagacatc
      541 cgtggtttcc tcacctccac catacacggc ctatgctgca ccggcccctg agcaggctta
      601 tggctatggg ccatacggtg gtgcgtaccc gccaggaact caagttgtct acgctgcgaa
      661 tgggcaggcg tatgccgtgc cctaccagta cccatatgca ggactttatg gacagcagcc
      721 tgctaaccaa gtcatcattc gagagcgcta tcgagacaac gacagcgacc tggcactggg
      781 catgctggca ggagcagcca cgggcatggc cttagggtct ctattttggg tcttctaggg
      841 gcctcaaggt cttgatgtgc atagcttctg ataaccctgt gtgcaataat atgatttgca
      901 gggcatttct gtttgtgaca aaagttttta ataatagttt taatcattcc tttgaaagta
      961 gtgatgtcat aattgtacta atccacataa gtaccacaga gaagggtttg aactgtgcta
     1021 ttttgttcaa atgttgactc tccgggggca ctggctcatt ccaagactgt tcttgtgcaa
     1081 ctctcagaat accttatttg agcatacctg ttttgaaagg cattttcttt ttagagttag
     1141 gtgtagtgct taagggttaa tttattttca tgttatgcca gtaatatagt gttgtatgcc
     1201 tattgagtga ttgtggcaag aaaagctaca gcttctttgc gtttaacttt ttcaaaccac
     1261 agaccagaac tggttgcatg ttactttagg agttgtgggt tggtaagctc ccaggtactt
     1321 cccgaggcta tggtgtgaga gcccccgtcc tgccctctgg ggctccacag gcccctggca
     1381 aggccgatgg ctcaggatga tggggcacag cccgcctttg aacaatcatg cttcagaaat
     1441 ctgcctgacc ctagctgctg ctgctgctca ctttattctt gtatggcttt ggtaggcata
     1501 cttggagaac atatcccaca ttaggaattg atttaagcct gagagtttga gggctttaat
     1561 cctttaaaac ttggagaagc tggctgggcg cggtggctca cgcctgtaat cccagcactt
     1621 tgagagaccg aggcgggcgg atcacgaggt caggagatcg agaccatcct ggctaacacg
     1681 gtgaaacccc atctctacta aaaatacaaa aaattagctg ggcgtggtgg caggcgcctg
     1741 tggtcccagc tactcgggag gctgaggcag gagaatagtg tgaacccagg aggcggagct
     1801 tgcagtgagc caagatagtg ccactgcact tcagcctggg tgacagagtg agactctgtc
     1861 tcaaaaaaaa aaaaaaaatc ctggagaagc cagaacaata tacaaacaag tatgtggagg
     1921 cagatttgct ttattccaag aggctgtttg agtgtgtgtc tgcctaagcc tccttatagc
     1981 ctatttttct acttgctgag agagtaatat taaaggaaca gtgagggagt ggaaggagag
     2041 ccttagttag agcgttccca tttctggcct ttgggggttt gcactgtgcg ggagatgctc
     2101 tctcatcact ctgaaggccc atcctgtgca gaagtgggac tctgtggctg ttcactgagc
     2161 accagggcag actgtagctg agcagacttg ggttatggat gggatatggg gcccagggac
     2221 cctcaggaca cagggaccgg ctcaaggtac cactcagcct ggccaggacc cccagctttg
     2281 cttttgttct tgatctcttt gtgatagaga agcttagaac attgaatttg gaaccccaaa
     2341 atgtgtgagt ctttggaaaa cccattccag cctttcttca aatgctctta ttttcagtat
     2401 atgaagtgtg tatttgaaat attcatattt ctgttatttc ttgttttcaa gctctgaatt
     2461 attgcagttt tctgtcctgc atgctttcaa cagtgtgctt ttacagtggc agtttagcac
     2521 agcgaatgtc cctgccccac actccatatg gtcctgtttt cctgaaaata cactccaggt
     2581 agcacagact tgttattttg cctggctggt tacacacatg ctgtcttgct ttgctgctca
     2641 gccacctccc tctgaggcca acttaggcca acctacatag gactccatct ttagcgagac
     2701 accagagaca cctagtgatg accacgggtc tgcttttctc tctgatccta ggggacttgt
     2761 tgacatggga gtgtctgttt ccacatggaa ggatgtctgt ttatctctca tctctgaata
     2821 gatgtatgac aactctagtg tccatggttt aaaatgttgc tttgaggatt ttccatcctg
     2881 tataagaaga atggctttct tcagattata atcgttatta aagctgtatg tacactttac
     2941 ttaaaaacta ttaacagttt ttcatgttgc actggtggta attttgaact tggaattact
     3001 gggtgggaat tccaggaacc acagagtatt gatttttgct gccaaaatgc tcttgaagca
     3061 gatgtccctg tgctcccctg gctgcttctg gctgaagggg ggaggtgtag actgaagctt
     3121 gggcactcat gtgtgtcccc tccccagtcc ccatcctagt ggggccagtc tcattaggca
     3181 gccatagata agcctggaac ttggctgtca ttagtgactt gatcctggta tgaaatgcat
     3241 actgggtata aggttgctca ggtattttat ttccttggcc acaactccca tagatgccaa
     3301 tgttttgata gcctcagttt ctcaacgatg tcttttgttt acagtgctac acttagtgtg
     3361 agtcaagaag tgcttgagtt acaagaaact tcttattcag gtttgagtaa gcattttact
     3421 tccatgtgta cttttaaata atccatacct tgaagaagtt ggccaatgat attatataca
     3481 tgctgatctg attgttcttt tcattccttg agtcaacttc agggtcttgg atactaaaga
     3541 gaaggagaac tgtggttaat gttttggatc atcatttgtg aacaaggcca gactttgccc
     3601 attttaggct atccaggact taaggtagcc atctggatca taagaaggtc ctgcagttga
     3661 gacaaaatga cccaagtgta ttttccacaa gttttgatcc tgtgtaatat ttctttttcc
     3721 ttccctagtg tatgtagata caaggcatgg aatgcagttc cactttctcc ccagagttga
     3781 tctttttgtg attgctttcc cgtctctagt agtattctgt tgtgtctaga gaactgattt
     3841 tttcctacat acgcaaattg tacatttgta agtgaaaatg tcaatacatt aaagcattaa
     3901 ccttaaagca
//
