LOCUS       NM_001100623            3812 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens pleckstrin homology domain containing B2 (PLEKHB2),
            transcript variant 3, mRNA.
ACCESSION   NM_001100623
VERSION     NM_001100623.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Matsudaira,T., Mukai,K., Noguchi,T., Hasegawa,J., Hatta,T.,
            Iemura,S.I., Natsume,T., Miyamura,N., Nishina,H., Nakayama,J.,
            Semba,K., Tomita,T., Murata,S., Arai,H. and Taguchi,T.
  TITLE     Endosomal phosphatidylserine is critical for the YAP signalling
            pathway in proliferating cells
  JOURNAL   Nat Commun 8 (1), 1246 (2017)
   PUBMED   29093443
  REMARK    GeneRIF: Knockdown of evectin-2 (a recycling endosome-resident
            protein) suppresses nuclear localization of YAP and YAP-dependent
            transcription. Evectin-2 knockdown reduces the ubiquitination and
            increased the level of Lats1.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3812)
  AUTHORS   Okazaki,S., Kato,R., Uchida,Y., Taguchi,T., Arai,H. and
            Wakatsuki,S.
  TITLE     Structural basis of the strict phospholipid binding specificity of
            the pleckstrin homology domain of human evectin-2
  JOURNAL   Acta Crystallogr D Biol Crystallogr 68 (Pt 2), 117-123 (2012)
   PUBMED   22281740
  REMARK    GeneRIF: The crystal structure at 1.75 A resolution of an apo form
            of human evectin-2 pleckstrin homology domain, in which the
            ligand-binding site is free from crystal packing and is thus
            appropriate for comparison with the structure of the complex, is
            reported.
REFERENCE   4  (bases 1 to 3812)
  AUTHORS   Uchida,Y., Hasegawa,J., Chinnapen,D., Inoue,T., Okazaki,S.,
            Kato,R., Wakatsuki,S., Misaki,R., Koike,M., Uchiyama,Y., Iemura,S.,
            Natsume,T., Kuwahara,R., Nakagawa,T., Nishikawa,K., Mukai,K.,
            Miyoshi,E., Taniguchi,N., Sheff,D., Lencer,W.I., Taguchi,T. and
            Arai,H.
  TITLE     Intracellular phosphatidylserine is essential for retrograde
            membrane traffic through endosomes
  JOURNAL   Proc Natl Acad Sci U S A 108 (38), 15846-15851 (2011)
   PUBMED   21911378
  REMARK    GeneRIF: Data show that depletion of evt-2 or masking of
            intracellular PS suppressed membrane traffic from REs to the Golgi.
REFERENCE   5  (bases 1 to 3812)
  AUTHORS   Girard,A., Sachidanandam,R., Hannon,G.J. and Carmell,M.A.
  TITLE     A germline-specific class of small RNAs binds mammalian Piwi
            proteins
  JOURNAL   Nature 442 (7099), 199-202 (2006)
   PUBMED   16751776
REFERENCE   6  (bases 1 to 3812)
  AUTHORS   Colland,F., Jacq,X., Trouplin,V., Mougin,C., Groizeleau,C.,
            Hamburger,A., Meil,A., Wojcik,J., Legrain,P. and Gauthier,J.M.
  TITLE     Functional proteomics mapping of a human signaling pathway
  JOURNAL   Genome Res 14 (7), 1324-1332 (2004)
   PUBMED   15231748
REFERENCE   7  (bases 1 to 3812)
  AUTHORS   Lehner,B. and Sanderson,C.M.
  TITLE     A protein interaction framework for human mRNA degradation
  JOURNAL   Genome Res 14 (7), 1315-1323 (2004)
   PUBMED   15231747
REFERENCE   8  (bases 1 to 3812)
  AUTHORS   Dowler,S., Currie,R.A., Campbell,D.G., Deak,M., Kular,G.,
            Downes,C.P. and Alessi,D.R.
  TITLE     Identification of pleckstrin-homology-domain-containing proteins
            with novel phosphoinositide-binding specificities
  JOURNAL   Biochem J 351 (Pt 1), 19-31 (2000)
   PUBMED   11001876
REFERENCE   9  (bases 1 to 3812)
  AUTHORS   Krappa,R., Nguyen,A., Burrola,P., Deretic,D. and Lemke,G.
  TITLE     Evectins: vesicular proteins that carry a pleckstrin homology
            domain and localize to post-Golgi membranes
  JOURNAL   Proc Natl Acad Sci U S A 96 (8), 4633-4638 (1999)
   PUBMED   10200314
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK126289.1, BC050695.1, DR761393.1, AK054998.1 and AI458777.1.
            
            On Aug 13, 2020 this sequence version replaced NM_001100623.1.
            
            Transcript Variant: This variant (3) lacks an in-frame exon in the
            3' coding region, compared to variant 4, which results in a protein
            (isoform 3) with a shorter C-terminus, compared to isoform 4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853561.16768.1,
                                           SRR11853558.14653.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000693505.1/ ENSP00000510611.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                AK126289.1         490-505
            17-1606             BC050695.1         1-1590
            1607-1887           DR761393.1         219-499
            1888-3379           AK054998.1         1-1492
            3380-3812           AI458777.1         26-458              c
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q21.1"
     gene            1..3812
                     /gene="PLEKHB2"
                     /gene_synonym="EVT2"
                     /note="pleckstrin homology domain containing B2"
                     /db_xref="GeneID:55041"
                     /db_xref="HGNC:HGNC:19236"
                     /db_xref="MIM:618452"
     exon            1..63
                     /gene="PLEKHB2"
                     /gene_synonym="EVT2"
                     /inference="alignment:Splign:2.1.0"
     exon            64..108
                     /gene="PLEKHB2"
                     /gene_synonym="EVT2"
                     /inference="alignment:Splign:2.1.0"
     CDS             72..740
                     /gene="PLEKHB2"
                     /gene_synonym="EVT2"
                     /note="isoform 3 is encoded by transcript variant 3;
                     pleckstrin homology domain-containing family B member 2;
                     evectin-2; PH domain-containing family B member 2;
                     pleckstrin homology domain containing, family B (evectins)
                     member 2"
                     /codon_start=1
                     /product="pleckstrin homology domain-containing family B
                     member 2 isoform 3"
                     /protein_id="NP_001094093.1"
                     /db_xref="CCDS:CCDS46413.1"
                     /db_xref="GeneID:55041"
                     /db_xref="HGNC:HGNC:19236"
                     /db_xref="MIM:618452"
                     /translation="MAFVKSGWLLRQSTILKRWKKNWFDLWSDGHLIYYDDQTRQNIE
                     DKVHMPMDCINIRTGQECRDTQPPDGKSKDCMLQIVCRDGKTISLCAESTDDCLAWKF
                     TLQDSRTNTAYVGSAVMTDETSVVSSPPPYTAYAAPAPEQAYGYGPYGGAYPPGTQVV
                     YAANGQAYAVPYQYPYAGLYGQQPANQVIIRERYRDNDSDLALGMLAGAATGMALGSL
                     FWVF"
     exon            109..261
                     /gene="PLEKHB2"
                     /gene_synonym="EVT2"
                     /inference="alignment:Splign:2.1.0"
     exon            262..364
                     /gene="PLEKHB2"
                     /gene_synonym="EVT2"
                     /inference="alignment:Splign:2.1.0"
     exon            365..404
                     /gene="PLEKHB2"
                     /gene_synonym="EVT2"
                     /inference="alignment:Splign:2.1.0"
     exon            405..494
                     /gene="PLEKHB2"
                     /gene_synonym="EVT2"
                     /inference="alignment:Splign:2.1.0"
     exon            495..603
                     /gene="PLEKHB2"
                     /gene_synonym="EVT2"
                     /inference="alignment:Splign:2.1.0"
     exon            604..3812
                     /gene="PLEKHB2"
                     /gene_synonym="EVT2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3790..3795
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLEKHB2"
                     /gene_synonym="EVT2"
                     /note="hexamer: ATTAAA"
     polyA_site      3812
                     /gene="PLEKHB2"
                     /gene_synonym="EVT2"
                     /note="major polyA site"
ORIGIN      
        1 agttgcaggc gagcaggcga ggaatcgccg tggcgtcttg gtgttctcca cgctggttcg
       61 caggtgaaga gatggcgttt gtgaagagtg gctggttgct gcgacagagt actattttga
      121 agcgctggaa gaagaactgg tttgatctgt ggtcggatgg tcacctgatc tattatgatg
      181 accagactcg gcagaatatc gaggataagg tccacatgcc aatggactgc atcaacatcc
      241 gcacggggca ggaatgtcgg gatactcagc ccccggatgg aaagtcaaaa gactgcatgc
      301 tccagattgt ttgtcgagat gggaaaacaa ttagtctttg tgcagaaagc acagatgatt
      361 gcttggcctg gaaatttaca ctccaagatt ctaggacaaa cacagcgtat gtgggctctg
      421 cagtcatgac cgatgagaca tccgtggttt cctcacctcc accatacacg gcctatgctg
      481 caccggcccc tgagcaggct tatggctatg ggccatacgg tggtgcgtac ccgccaggaa
      541 ctcaagttgt ctacgctgcg aatgggcagg cgtatgccgt gccctaccag tacccatatg
      601 caggacttta tggacagcag cctgctaacc aagtcatcat tcgagagcgc tatcgagaca
      661 acgacagcga cctggcactg ggcatgctgg caggagcagc cacgggcatg gccttagggt
      721 ctctattttg ggtcttctag gggcctcaag gtcttgatgt gcatagcttc tgataaccct
      781 gtgtgcaata atatgatttg cagggcattt ctgtttgtga caaaagtttt taataatagt
      841 tttaatcatt cctttgaaag tagtgatgtc ataattgtac taatccacat aagtaccaca
      901 gagaagggtt tgaactgtgc tattttgttc aaatgttgac tctccggggg cactggctca
      961 ttccaagact gttcttgtgc aactctcaga ataccttatt tgagcatacc tgttttgaaa
     1021 ggcattttct ttttagagtt aggtgtagtg cttaagggtt aatttatttt catgttatgc
     1081 cagtaatata gtgttgtatg cctattgagt gattgtggca agaaaagcta cagcttcttt
     1141 gcgtttaact ttttcaaacc acagaccaga actggttgca tgttacttta ggagttgtgg
     1201 gttggtaagc tcccaggtac ttcccgaggc tatggtgtga gagcccccgt cctgccctct
     1261 ggggctccac aggcccctgg caaggccgat ggctcaggat gatggggcac agcccgcctt
     1321 tgaacaatca tgcttcagaa atctgcctga ccctagctgc tgctgctgct cactttattc
     1381 ttgtatggct ttggtaggca tacttggaga acatatccca cattaggaat tgatttaagc
     1441 ctgagagttt gagggcttta atcctttaaa acttggagaa gctggctggg cgcggtggct
     1501 cacgcctgta atcccagcac tttgagagac cgaggcgggc ggatcacgag gtcaggagat
     1561 cgagaccatc ctggctaaca cggtgaaacc ccatctctac taaaaataca aaaaattagc
     1621 tgggcgtggt ggcaggcgcc tgtggtccca gctactcggg aggctgaggc aggagaatag
     1681 tgtgaaccca ggaggcggag cttgcagtga gccaagatag tgccactgca cttcagcctg
     1741 ggtgacagag tgagactctg tctcaaaaaa aaaaaaaaaa tcctggagaa gccagaacaa
     1801 tatacaaaca agtatgtgga ggcagatttg ctttattcca agaggctgtt tgagtgtgtg
     1861 tctgcctaag cctccttata gcctattttt ctacttgctg agagagtaat attaaaggaa
     1921 cagtgaggga gtggaaggag agccttagtt agagcgttcc catttctggc ctttgggggt
     1981 ttgcactgtg cgggagatgc tctctcatca ctctgaaggc ccatcctgtg cagaagtggg
     2041 actctgtggc tgttcactga gcaccagggc agactgtagc tgagcagact tgggttatgg
     2101 atgggatatg gggcccaggg accctcagga cacagggacc ggctcaaggt accactcagc
     2161 ctggccagga cccccagctt tgcttttgtt cttgatctct ttgtgataga gaagcttaga
     2221 acattgaatt tggaacccca aaatgtgtga gtctttggaa aacccattcc agcctttctt
     2281 caaatgctct tattttcagt atatgaagtg tgtatttgaa atattcatat ttctgttatt
     2341 tcttgttttc aagctctgaa ttattgcagt tttctgtcct gcatgctttc aacagtgtgc
     2401 ttttacagtg gcagtttagc acagcgaatg tccctgcccc acactccata tggtcctgtt
     2461 ttcctgaaaa tacactccag gtagcacaga cttgttattt tgcctggctg gttacacaca
     2521 tgctgtcttg ctttgctgct cagccacctc cctctgaggc caacttaggc caacctacat
     2581 aggactccat ctttagcgag acaccagaga cacctagtga tgaccacggg tctgcttttc
     2641 tctctgatcc taggggactt gttgacatgg gagtgtctgt ttccacatgg aaggatgtct
     2701 gtttatctct catctctgaa tagatgtatg acaactctag tgtccatggt ttaaaatgtt
     2761 gctttgagga ttttccatcc tgtataagaa gaatggcttt cttcagatta taatcgttat
     2821 taaagctgta tgtacacttt acttaaaaac tattaacagt ttttcatgtt gcactggtgg
     2881 taattttgaa cttggaatta ctgggtggga attccaggaa ccacagagta ttgatttttg
     2941 ctgccaaaat gctcttgaag cagatgtccc tgtgctcccc tggctgcttc tggctgaagg
     3001 ggggaggtgt agactgaagc ttgggcactc atgtgtgtcc cctccccagt ccccatccta
     3061 gtggggccag tctcattagg cagccataga taagcctgga acttggctgt cattagtgac
     3121 ttgatcctgg tatgaaatgc atactgggta taaggttgct caggtatttt atttccttgg
     3181 ccacaactcc catagatgcc aatgttttga tagcctcagt ttctcaacga tgtcttttgt
     3241 ttacagtgct acacttagtg tgagtcaaga agtgcttgag ttacaagaaa cttcttattc
     3301 aggtttgagt aagcatttta cttccatgtg tacttttaaa taatccatac cttgaagaag
     3361 ttggccaatg atattatata catgctgatc tgattgttct tttcattcct tgagtcaact
     3421 tcagggtctt ggatactaaa gagaaggaga actgtggtta atgttttgga tcatcatttg
     3481 tgaacaaggc cagactttgc ccattttagg ctatccagga cttaaggtag ccatctggat
     3541 cataagaagg tcctgcagtt gagacaaaat gacccaagtg tattttccac aagttttgat
     3601 cctgtgtaat atttcttttt ccttccctag tgtatgtaga tacaaggcat ggaatgcagt
     3661 tccactttct ccccagagtt gatctttttg tgattgcttt cccgtctcta gtagtattct
     3721 gttgtgtcta gagaactgat tttttcctac atacgcaaat tgtacatttg taagtgaaaa
     3781 tgtcaataca ttaaagcatt aaccttaaag ca
//
