LOCUS       NM_016445               1513 bp    mRNA    linear   PRI 09-OCT-2024
DEFINITION  Homo sapiens pleckstrin 2 (PLEK2), mRNA.
ACCESSION   NM_016445
VERSION     NM_016445.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Han,X., Zhang,A., Wang,P., Bi,H., Ren,K., Li,E., Yang,X.,
            Aydemir,I., Tao,K., Lin,J., Abdulkadir,S.A., Yang,J. and Ji,P.
  TITLE     Pleckstrin-2 Mediates the Activation of AKT in Prostate Cancer and
            Is Repressed by Androgen Receptor
  JOURNAL   Am J Pathol 194 (10), 1986-1996 (2024)
   PUBMED   39069167
  REMARK    GeneRIF: Pleckstrin-2 Mediates the Activation of AKT in Prostate
            Cancer and Is Repressed by Androgen Receptor.
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Zhang,W., Yu,L., Xu,C., Tang,T., Cao,J., Chen,L., Pang,X. and
            Ren,W.
  TITLE     PLEK2 activates the PI3K/AKT signaling pathway to drive lung
            adenocarcinoma progression by upregulating SPC25
  JOURNAL   Cell Biol Int 48 (9), 1285-1300 (2024)
   PUBMED   38894536
  REMARK    GeneRIF: PLEK2 activates the PI3K/AKT signaling pathway to drive
            lung adenocarcinoma progression by upregulating SPC25.
REFERENCE   3  (bases 1 to 1513)
  AUTHORS   Li,Q., Li,J., Wang,J., Wang,J., Lu,T., Jia,Y., Sun,H. and Ma,X.
  TITLE     PLEK2 mediates metastasis and invasion via alpha5-nAChR activation
            in nicotine-induced lung adenocarcinoma
  JOURNAL   Mol Carcinog 63 (2), 253-265 (2024)
   PUBMED   37921560
  REMARK    GeneRIF: PLEK2 mediates metastasis and invasion via alpha5-nAChR
            activation in nicotine-induced lung adenocarcinoma.
REFERENCE   4  (bases 1 to 1513)
  AUTHORS   Mao,D., Zhou,Z., Chen,H., Liu,X., Li,D., Chen,X., He,Y., Liu,M. and
            Zhang,C.
  TITLE     Pleckstrin-2 promotes tumour immune escape from NK cells by
            activating the MT1-MMP-MICA signalling axis in gastric cancer
  JOURNAL   Cancer Lett 572, 216351 (2023)
   PUBMED   37591356
  REMARK    GeneRIF: Pleckstrin-2 promotes tumour immune escape from NK cells
            by activating the MT1-MMP-MICA signalling axis in gastric cancer.
REFERENCE   5  (bases 1 to 1513)
  AUTHORS   Du,L., Li,J., Tian,Y. and Feng,R.
  TITLE     Pleckstrin-2-promoted PPM1B degradation plays an important role in
            transforming growth factor-beta-induced breast cancer cell invasion
            and metastasis
  JOURNAL   Cancer Sci 114 (6), 2429-2444 (2023)
   PUBMED   36928924
  REMARK    GeneRIF: Pleckstrin-2-promoted PPM1B degradation plays an important
            role in transforming growth factor-beta-induced breast cancer cell
            invasion and metastasis.
REFERENCE   6  (bases 1 to 1513)
  AUTHORS   Bach,T.L., Kerr,W.T., Wang,Y., Bauman,E.M., Kine,P., Whiteman,E.L.,
            Morgan,R.S., Williamson,E.K., Ostap,E.M., Burkhardt,J.K.,
            Koretzky,G.A., Birnbaum,M.J. and Abrams,C.S.
  TITLE     PI3K regulates pleckstrin-2 in T-cell cytoskeletal reorganization
  JOURNAL   Blood 109 (3), 1147-1155 (2007)
   PUBMED   17008542
  REMARK    GeneRIF: pleckstrin-2 uses its modular domains to bind to
            membrane-associated phosphatidylinositols generated by PI3K,
            whereby it coordinates with the actin cytoskeleton in lymphocyte
            spreading and immune synapse formation.
REFERENCE   7  (bases 1 to 1513)
  AUTHORS   Inazu,T., Kuroiwa,A., Matsuda,Y. and Miyamoto,K.
  TITLE     Cloning, expression and chromosomal assignment of human pleckstrin
            2
  JOURNAL   Mol Biol Rep 32 (1), 35-40 (2005)
   PUBMED   15865208
  REMARK    GeneRIF: Pleckstrin 2 was cloned; its mRNA is widely expressed in a
            variety of cell lines.
REFERENCE   8  (bases 1 to 1513)
  AUTHORS   Lemmon,M.A., Ferguson,K.M. and Abrams,C.S.
  TITLE     Pleckstrin homology domains and the cytoskeleton
  JOURNAL   FEBS Lett 513 (1), 71-76 (2002)
   PUBMED   11911883
  REMARK    Review article
REFERENCE   9  (bases 1 to 1513)
  AUTHORS   Inazu,T., Yamada,K. and Miyamoto,K.
  TITLE     Cloning and expression of pleckstrin 2, a novel member of the
            pleckstrin family
  JOURNAL   Biochem Biophys Res Commun 265 (1), 87-93 (1999)
   PUBMED   10548495
REFERENCE   10 (bases 1 to 1513)
  AUTHORS   Hu,M.H., Bauman,E.M., Roll,R.L., Yeilding,N. and Abrams,C.S.
  TITLE     Pleckstrin 2, a widely expressed paralog of pleckstrin involved in
            actin rearrangement
  JOURNAL   J Biol Chem 274 (31), 21515-21518 (1999)
   PUBMED   10419454
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CR976852.1 and AF228603.1.
            
            On Nov 22, 2018 this sequence version replaced NM_016445.2.
            
            Summary: The protein encoded by this gene associates with
            membrane-bound phosphatidylinositols generated by
            phosphatidylinositol 3-kinase. The encoded protein then interacts
            with the actin cytoskeleton to induce cell spreading. In
            conjunction with complement component 1, q subcomponent, B chain
            (C1QB), this gene shows an increase in expression in melanoma cells
            and may serve as an accurate biomarker for the disease. [provided
            by RefSeq, Dec 2015].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189658.122977.1, BC001226.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145893, SAMEA2146411
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000216446.9/ ENSP00000216446.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-54                CR976852.1         40-93
            55-1513             AF228603.1         1-1459
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q23.3-q24.1"
     gene            1..1513
                     /gene="PLEK2"
                     /note="pleckstrin 2"
                     /db_xref="GeneID:26499"
                     /db_xref="HGNC:HGNC:19238"
                     /db_xref="MIM:608007"
     exon            1..148
                     /gene="PLEK2"
                     /inference="alignment:Splign:2.1.0"
     CDS             107..1168
                     /gene="PLEK2"
                     /note="pleckstrin 2 homolog"
                     /codon_start=1
                     /product="pleckstrin-2"
                     /protein_id="NP_057529.1"
                     /db_xref="CCDS:CCDS9782.1"
                     /db_xref="GeneID:26499"
                     /db_xref="HGNC:HGNC:19238"
                     /db_xref="MIM:608007"
                     /translation="MEDGVLKEGFLVKRGHIVHNWKARWFILRQNTLVYYKLEGGRRV
                     TPPKGRILLDGCTITCPCLEYENRPLLIKLKTQTSTEYFLEACSREERDAWAFEITGA
                     IHAGQPGKVQQLHSLRNSFKLPPHISLHRIVDKMHDSNTGIRSSPNMEQGSTYKKTFL
                     GSSLVDWLISNSFTASRLEAVTLASMLMEENFLRPVGVRSMGAIRSGDLAEQFLDDST
                     ALYTFAESYKKKISPKEEISLSTVELSGTVVKQGYLAKQGHKRKNWKVRRFVLRKDPA
                     FLHYYDPSKEENRPVGGFSLRGSLVSALEDNGVPTGVKGNVQGNLFKVITKDDTHYYI
                     QASSKAERAEWIEAIKKLT"
     exon            149..313
                     /gene="PLEK2"
                     /inference="alignment:Splign:2.1.0"
     exon            314..495
                     /gene="PLEK2"
                     /inference="alignment:Splign:2.1.0"
     exon            496..587
                     /gene="PLEK2"
                     /inference="alignment:Splign:2.1.0"
     exon            588..775
                     /gene="PLEK2"
                     /inference="alignment:Splign:2.1.0"
     exon            776..877
                     /gene="PLEK2"
                     /inference="alignment:Splign:2.1.0"
     exon            878..961
                     /gene="PLEK2"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1040
                     /gene="PLEK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1513
                     /gene="PLEK2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1493..1498
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLEK2"
                     /note="hexamer: AATAAA"
     polyA_site      1513
                     /gene="PLEK2"
                     /note="major polyA site"
ORIGIN      
        1 gcttcctggg cgccgtgggc gcggactgcg cgggctgcgc gggtgccgag gagcgcgagg
       61 cgcggggaag gcgcacctgg ggtggccctg gcgtgcgggc ggcgacatgg aggacggcgt
      121 gctcaaggag ggcttcctgg tcaagagggg ccacattgtc cacaactgga aggcgcgatg
      181 gttcatcctt cggcagaaca cgctggtgta ctacaagctt gaggggggtc ggagagtgac
      241 ccctcccaag ggccggatcc tcctggatgg ctgcaccatc acctgcccct gcctggagta
      301 tgaaaaccga ccgctcctca ttaagctgaa gactcaaaca tccacggagt acttcctgga
      361 ggcctgttct cgagaggagc gggatgcctg ggcctttgag atcaccgggg ctattcatgc
      421 agggcagccg gggaaggtcc agcagctgca cagcctgaga aactccttca agctgccccc
      481 gcacatcagc ctgcatcgca ttgtggacaa gatgcacgat agcaacaccg gaatccgttc
      541 aagccccaac atggagcagg gaagcaccta taaaaagacc ttcctcggct cctccctggt
      601 ggactggctc atctccaaca gcttcacggc cagccgtctg gaggcggtga ccctggcctc
      661 catgctcatg gaggagaact tcctcaggcc tgtgggtgtc cgaagcatgg gagccattcg
      721 ctctggggat ctggccgagc agttcctgga tgactccaca gccctgtaca cttttgctga
      781 gagctacaaa aagaagataa gccccaagga agaaattagc ctgagcactg tggagttaag
      841 tggcacggtg gtgaaacaag gctacctggc caagcaggga cacaagagga aaaactggaa
      901 ggtgcgtcgc tttgttctaa ggaaggatcc agctttcctg cattactatg acccttccaa
      961 agaagagaac aggccagtgg gtgggttttc tcttcgtggt tcactcgtgt ctgctctgga
     1021 agataatggc gttcccactg gggttaaagg gaatgtccag ggaaacctct tcaaagtgat
     1081 tactaaggat gacacacact attacattca ggccagcagc aaggctgagc gagccgagtg
     1141 gattgaagct atcaaaaagc taacatgaca aggacctgag ggaaccagga ttcctccctc
     1201 ctaccagatg acacagacaa gagttcctgg agaatgggag tgttaagact tttgacttct
     1261 ttgtaagttt tgtactgctt tggagagtga atgctgccaa gagttcctca gattacaaac
     1321 agcagtggtg ccatttcctt ccccatcttc atgttacaaa cctggaaagg ctagaacagc
     1381 cattaggcgt cagcatcttg acttttcccc agcatcacaa acagccattt cctcgggcac
     1441 caaagtaggt tccctttgtt ggaacaatta cactggccat gccataatgt tgaataaaac
     1501 tctcttctta tga
//
