LOCUS       NM_002664               2760 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens pleckstrin (PLEK), mRNA.
ACCESSION   NM_002664
VERSION     NM_002664.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Zhao,M., Liu,A., Mo,L., Wan,G., Lu,F., Chen,L., Fu,S., Chen,H.,
            Fu,T. and Deng,H.
  TITLE     Higher expression of PLEK and LY86 as the potential biomarker of
            carotid atherosclerosis
  JOURNAL   Medicine (Baltimore) 102 (42), e34445 (2023)
   PUBMED   37861500
  REMARK    GeneRIF: Higher expression of PLEK and LY86 as the potential
            biomarker of carotid atherosclerosis.
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Alim,M.A., Njenda,D., Lundmark,A., Kaminska,M., Jansson,L.,
            Eriksson,K., Kats,A., Johannsen,G., Arvidsson,C.K., Mydel,P.M. and
            Yucel-Lindberg,T.
  TITLE     Pleckstrin Levels Are Increased in Patients with Chronic
            Periodontitis and Regulated via the MAP Kinase-p38alpha Signaling
            Pathway in Gingival Fibroblasts
  JOURNAL   Front Immunol 12, 801096 (2022)
   PUBMED   35087525
  REMARK    GeneRIF: Pleckstrin Levels Are Increased in Patients with Chronic
            Periodontitis and Regulated via the MAP Kinase-p38alpha Signaling
            Pathway in Gingival Fibroblasts.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2760)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2760)
  AUTHORS   Lezirovitz,K., Vieira-Silva,G.A., Batissoco,A.C., Levy,D.,
            Kitajima,J.P., Trouillet,A., Ouyang,E., Zebarjadi,N.,
            Sampaio-Silva,J., Pedroso-Campos,V., Nascimento,L.R., Sonoda,C.Y.,
            Borges,V.M., Vasconcelos,L.G., Beck,R.M.O., Grasel,S.S.,
            Jagger,D.J., Grillet,N., Bento,R.F., Mingroni-Netto,R.C. and
            Oiticica,J.
  TITLE     A rare genomic duplication in 2p14 underlies autosomal dominant
            hearing loss DFNA58
  JOURNAL   Hum Mol Genet 29 (9), 1520-1536 (2020)
   PUBMED   32337552
  REMARK    GeneRIF: A rare genomic duplication in 2p14 underlies autosomal
            dominant hearing loss DFNA58.
REFERENCE   5  (bases 1 to 2760)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   6  (bases 1 to 2760)
  AUTHORS   Abrams,C.S., Zhao,W., Belmonte,E. and Brass,L.F.
  TITLE     Protein kinase C regulates pleckstrin by phosphorylation of sites
            adjacent to the N-terminal pleckstrin homology domain
  JOURNAL   J Biol Chem 270 (40), 23317-23321 (1995)
   PUBMED   7559487
REFERENCE   7  (bases 1 to 2760)
  AUTHORS   Tyers,M., Haslam,R.J., Rachubinski,R.A. and Harley,C.B.
  TITLE     Molecular analysis of pleckstrin: the major protein kinase C
            substrate of platelets
  JOURNAL   J Cell Biochem 40 (2), 133-145 (1989)
   PUBMED   2768345
REFERENCE   8  (bases 1 to 2760)
  AUTHORS   Tyers,M., Rachubinski,R.A., Stewart,M.I., Varrichio,A.M.,
            Shorr,R.G., Haslam,R.J. and Harley,C.B.
  TITLE     Molecular cloning and expression of the major protein kinase C
            substrate of platelets
  JOURNAL   Nature 333 (6172), 470-473 (1988)
   PUBMED   2897630
REFERENCE   9  (bases 1 to 2760)
  AUTHORS   Imaoka,T., Lynham,J.A. and Haslam,R.J.
  TITLE     Purification and characterization of the 47,000-dalton protein
            phosphorylated during degranulation of human platelets
  JOURNAL   J Biol Chem 258 (18), 11404-11414 (1983)
   PUBMED   6885823
REFERENCE   10 (bases 1 to 2760)
  AUTHORS   Gogstad,G.O., Hagen,I., Korsmo,R. and Solum,N.O.
  TITLE     Characterization of the proteins of isolated human platelet
            alpha-granules. Evidence for a separate alpha-granule-pool of the
            glycoproteins IIb and IIIa
  JOURNAL   Biochim Biophys Acta 670 (2), 150-162 (1981)
   PUBMED   6457647
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BM477542.1, BC018549.1, AK130678.1 and AC015969.9.
            
            On Nov 23, 2018 this sequence version replaced NM_002664.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC018549.1, SRR1660803.67273.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000234313.8/ ENSP00000234313.7
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 BM477542.1         110-111
            3-1088              BC018549.1         1-1086
            1089-1797           AK130678.1         410-1118
            1798-1826           BC018549.1         1794-1822
            1827-2027           AK130678.1         1148-1348
            2028-2753           AC015969.9         119954-120679
            2754-2760           BC018549.1         2749-2755
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p14"
     gene            1..2760
                     /gene="PLEK"
                     /gene_synonym="P47; PLEK1"
                     /note="pleckstrin"
                     /db_xref="GeneID:5341"
                     /db_xref="HGNC:HGNC:9070"
                     /db_xref="MIM:173570"
     exon            1..112
                     /gene="PLEK"
                     /gene_synonym="P47; PLEK1"
                     /inference="alignment:Splign:2.1.0"
     CDS             71..1123
                     /gene="PLEK"
                     /gene_synonym="P47; PLEK1"
                     /note="platelet 47 kDa protein"
                     /codon_start=1
                     /product="pleckstrin"
                     /protein_id="NP_002655.2"
                     /db_xref="CCDS:CCDS1887.1"
                     /db_xref="GeneID:5341"
                     /db_xref="HGNC:HGNC:9070"
                     /db_xref="MIM:173570"
                     /translation="MEPKRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSP
                     KGMIPLKGSTLTSPCQDFGKRMFVFKITTTKQQDHFFQAAFLEERDAWVRDIKKAIKC
                     IEGGQKFARKSTRRSIRLPETIDLGALYLSMKDTEKGIKELNLEKDKKIFNHCFTGNC
                     VIDWLVSNQSVRNRQEGLMIASSLLNEGYLQPAGDMSKSAVDGTAENPFLDNPDAFYY
                     FPDSGFFCEENSSDDDVILKEEFRGVIIKQGCLLKQGHRRKNWKVRKFILREDPAYLH
                     YYDPAGAEDPLGAIHLRGCVVTSVESNSNGRKSEEENLFEIITADEVHYFLQAATPKE
                     RTEWIRAIQMASRTGK"
     exon            113..268
                     /gene="PLEK"
                     /gene_synonym="P47; PLEK1"
                     /inference="alignment:Splign:2.1.0"
     exon            269..450
                     /gene="PLEK"
                     /gene_synonym="P47; PLEK1"
                     /inference="alignment:Splign:2.1.0"
     exon            451..542
                     /gene="PLEK"
                     /gene_synonym="P47; PLEK1"
                     /inference="alignment:Splign:2.1.0"
     exon            543..727
                     /gene="PLEK"
                     /gene_synonym="P47; PLEK1"
                     /inference="alignment:Splign:2.1.0"
     exon            728..832
                     /gene="PLEK"
                     /gene_synonym="P47; PLEK1"
                     /inference="alignment:Splign:2.1.0"
     exon            833..916
                     /gene="PLEK"
                     /gene_synonym="P47; PLEK1"
                     /inference="alignment:Splign:2.1.0"
     exon            917..986
                     /gene="PLEK"
                     /gene_synonym="P47; PLEK1"
                     /inference="alignment:Splign:2.1.0"
     exon            987..2760
                     /gene="PLEK"
                     /gene_synonym="P47; PLEK1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2738..2743
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLEK"
                     /gene_synonym="P47; PLEK1"
                     /note="hexamer: AATATA"
     polyA_site      2760
                     /gene="PLEK"
                     /gene_synonym="P47; PLEK1"
                     /note="major polyA site"
ORIGIN      
        1 atgcagagga ggcccagctg ctgagaggag ttgcctgaga gtgacctttg catctgcctg
       61 tccagccagc atggaaccaa agcggatcag agagggctac cttgtgaaga aggggagcgt
      121 gttcaatacg tggaaaccca tgtgggttgt attgttagaa gatggaattg aattctataa
      181 gaagaaaagt gacaacagcc ccaaaggaat gatcccgctg aaagggagca ctctgactag
      241 cccttgtcaa gactttggca aaaggatgtt tgtgtttaag atcactacga ccaaacagca
      301 ggaccacttc ttccaggcag ccttcctgga ggagagagat gcctgggttc gggatatcaa
      361 gaaggccatt aaatgcattg aaggaggcca gaaatttgcc aggaaatcta ccaggaggtc
      421 cattcgactg ccagaaacca ttgacttagg tgccttatat ttgtccatga aagacactga
      481 aaaaggaata aaagaactga atctagagaa ggacaagaag atttttaatc actgcttcac
      541 aggtaactgc gtcattgatt ggctggtatc caaccagtct gttaggaatc gccaggaagg
      601 cctcatgatt gcttcatcgc tgctcaatga ggggtatctg cagcctgctg gagacatgtc
      661 caagagtgca gtggatggaa ctgctgaaaa ccctttcctg gacaaccctg atgccttcta
      721 ctactttcca gacagtgggt tcttctgtga agagaattcc agtgatgatg atgtgattct
      781 gaaagaagaa ttcagagggg tcattatcaa gcagggatgt ttactgaagc aggggcatag
      841 aaggaaaaac tggaaagtga ggaagttcat cttgagagaa gaccctgcct acctgcacta
      901 ctatgaccct gctggggcag aagatcccct gggagcaatt cacttgagag gctgtgtggt
      961 gacttcagtg gagagcaact caaatggcag gaagagtgag gaagagaacc tttttgagat
     1021 catcacagca gatgaagtgc actatttctt gcaagcagcc acccccaagg agcgcacaga
     1081 gtggatcaga gccatccaga tggcctcccg aactgggaag taaagagact cctgcattcc
     1141 tcctcccctc ctgagggaag cccatggaca agctcagtcc aggacctgtc cacttctgtg
     1201 acaaatcaac gggaaacagc ccaggggtgg gaagttttca tttgcagggg ggtctgaatg
     1261 taactcacca tgtggtgtgc aaggttcccc tgcattgtat tgctcactgc agcccctctg
     1321 cccctatcca tgacccccaa gcagatataa caagctgtgc agcctcagta ggctgcttgc
     1381 cctctccagg cctcagggcc tcttctggaa aatgaagaaa ttcaactagt agattcctga
     1441 ggtcccccta gcttaaaaaa aaaaaaaatc tgccccatga ttctaacact cgcagtagtg
     1501 atagtgtatc tagttgttct gctggtgtcc ttccttggct aagtcttggc cttcagttat
     1561 cttcaaatgt accagaacct gagccaacgc ctccctgtga aactgttgct gatctgtagt
     1621 acagtaccag gaagaaacct cttttgttct ctttagacat cttctacttg ctcttggcct
     1681 tgagatcgtg taacaaaatg aaggagggct ctcttctttc ttcctcatcc tactcaaaaa
     1741 cttcccgaga gcagtggtgg ttttgagggt tttgacttct attacttttg gcagcctgga
     1801 aagttgtgtc ttctgggaaa gagacctggg gaggccagga gtagctgagg gtcctttctg
     1861 tgcccttaaa ccgcccagag gagccctatt ccactctggt tttaggctga tctgagaggg
     1921 tctccctttg ttcctttctg gagcatttct ctaacgttta ttacaattag gagggggacc
     1981 ccacatctgt gagattctgt ttcatttgag gtttacagaa aaaaaaaagt ggccagatgt
     2041 gttcccccca tgggtgagag gcctgggcaa ctgcctggtg aatgtgtctt gcggcagctg
     2101 cagcaagtgg aggggctgaa ctactggcca gctcactgga tgatgggtta atacaacaac
     2161 tgcactgtaa ggactcagag ccacacagaa cttctgagag gggctgttag cattgcgcag
     2221 catcttcagt tctccagtaa atgatattgc gttcgtgcct cagctttaag cacaagtagc
     2281 agcagctcct gcttgagttc tgagggcatc atggccctat gattaaccag agtgatctaa
     2341 cctagactaa aattgggaac ttatttgcaa tttttgaccc tgaccactaa ctagtgattc
     2401 ttctccaaaa ttgagaaaga cagcacccat tgaagcagat atgtgtgtga aagtatattt
     2461 ttcaattcca gatttttaat tttaaggctc caggaaagaa aggagagtag aacatttttc
     2521 ctcattttat caaatcctct cttgccctcc ctcaattccc ctgtaacatt cctgaagctg
     2581 ttcccactcc cagatggttt tatcaatagc ctagaggtaa agaactgtct ttttctctga
     2641 ttctttaata aattatcttt atagaatatg cacaagtttt tctacactca gtgttaaagt
     2701 atttattaat gggaagtcaa cttaatgttt tgaaataaat atatgactct gtttaatgca
//
