LOCUS       NM_201384              14804 bp    mRNA    linear   PRI 11-OCT-2024
DEFINITION  Homo sapiens plectin (PLEC), transcript variant 11, mRNA.
ACCESSION   NM_201384
VERSION     NM_201384.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 14804)
  AUTHORS   Rezniczek,G.A., Walko,G. and Wiche,G.
  TITLE     Plectin gene defects lead to various forms of epidermolysis bullosa
            simplex
  JOURNAL   Dermatol Clin 28 (1), 33-41 (2010)
   PUBMED   19945614
  REMARK    Review article
            Erratum:[Dermatol Clin. 2010 Apr;28(2):439-41]
REFERENCE   2  (bases 1 to 14804)
  AUTHORS   Winter,L., Abrahamsberg,C. and Wiche,G.
  TITLE     Plectin isoform 1b mediates mitochondrion-intermediate filament
            network linkage and controls organelle shape
  JOURNAL   J Cell Biol 181 (6), 903-911 (2008)
   PUBMED   18541706
REFERENCE   3  (bases 1 to 14804)
  AUTHORS   Sonnenberg,A. and Liem,R.K.
  TITLE     Plakins in development and disease
  JOURNAL   Exp Cell Res 313 (10), 2189-2203 (2007)
   PUBMED   17499243
  REMARK    Review article
REFERENCE   4  (bases 1 to 14804)
  AUTHORS   Rezniczek,G.A., Konieczny,P., Nikolic,B., Reipert,S., Schneller,D.,
            Abrahamsberg,C., Davies,K.E., Winder,S.J. and Wiche,G.
  TITLE     Plectin 1f scaffolding at the sarcolemma of dystrophic (mdx) muscle
            fibers through multiple interactions with beta-dystroglycan
  JOURNAL   J Cell Biol 176 (7), 965-977 (2007)
   PUBMED   17389230
REFERENCE   5  (bases 1 to 14804)
  AUTHORS   Pfendner,E., Rouan,F. and Uitto,J.
  TITLE     Progress in epidermolysis bullosa: the phenotypic spectrum of
            plectin mutations
  JOURNAL   Exp Dermatol 14 (4), 241-249 (2005)
   PUBMED   15810881
  REMARK    GeneRIF: discussion of phenotypic spectrum of plectin mutations
            [review]
            Review article
REFERENCE   6  (bases 1 to 14804)
  AUTHORS   Zhang,T., Haws,P. and Wu,Q.
  TITLE     Multiple variable first exons: a mechanism for cell- and
            tissue-specific gene regulation
  JOURNAL   Genome Res 14 (1), 79-89 (2004)
   PUBMED   14672974
REFERENCE   7  (bases 1 to 14804)
  AUTHORS   Rezniczek,G.A., Abrahamsberg,C., Fuchs,P., Spazierer,D. and
            Wiche,G.
  TITLE     Plectin 5'-transcript diversity: short alternative sequences
            determine stability of gene products, initiation of translation and
            subcellular localization of isoforms
  JOURNAL   Hum Mol Genet 12 (23), 3181-3194 (2003)
   PUBMED   14559777
REFERENCE   8  (bases 1 to 14804)
  AUTHORS   Andra,K., Kornacker,I., Jorgl,A., Zorer,M., Spazierer,D., Fuchs,P.,
            Fischer,I. and Wiche,G.
  TITLE     Plectin-isoform-specific rescue of hemidesmosomal defects in
            plectin (-/-) keratinocytes
  JOURNAL   J Invest Dermatol 120 (2), 189-197 (2003)
   PUBMED   12542521
REFERENCE   9  (bases 1 to 14804)
  AUTHORS   Leung,C.L., Green,K.J. and Liem,R.K.
  TITLE     Plakins: a family of versatile cytolinker proteins
  JOURNAL   Trends Cell Biol 12 (1), 37-45 (2002)
   PUBMED   11854008
  REMARK    Review article
REFERENCE   10 (bases 1 to 14804)
  AUTHORS   Brown,M.J., Hallam,J.A., Liu,Y., Yamada,K.M. and Shaw,S.
  TITLE     Cutting edge: integration of human T lymphocyte cytoskeleton by the
            cytolinker plectin
  JOURNAL   J Immunol 167 (2), 641-645 (2001)
   PUBMED   11441066
  REMARK    GeneRIF: During polarization of peripheral blood T lymphocytes,
            plectin redistributes to the uropod associated with vimentin and
            fodrin. This vimentin-plectin-fodrin complex provides a continuous
            linkage from the nucleus (lamin B) to the cortical cytoskeleton.
REFERENCE   11 (bases 1 to 14804)
  AUTHORS   Schroder,R., Furst,D.O., Klasen,C., Reimann,J., Herrmann,H. and van
            der Ven,P.F.
  TITLE     Association of plectin with Z-discs is a prerequisite for the
            formation of the intermyofibrillar desmin cytoskeleton
  JOURNAL   Lab Invest 80 (4), 455-464 (2000)
   PUBMED   10780662
REFERENCE   12 (bases 1 to 14804)
  AUTHORS   Fuchs,P., Zorer,M., Rezniczek,G.A., Spazierer,D., Oehler,S.,
            Castanon,M.J., Hauptmann,R. and Wiche,G.
  TITLE     Unusual 5' transcript complexity of plectin isoforms: novel
            tissue-specific exons modulate actin binding activity
  JOURNAL   Hum Mol Genet 8 (13), 2461-2472 (1999)
   PUBMED   10556294
REFERENCE   13 (bases 1 to 14804)
  AUTHORS   Wiche,G.
  TITLE     Role of plectin in cytoskeleton organization and dynamics
  JOURNAL   J Cell Sci 111 (Pt 17), 2477-2486 (1998)
   PUBMED   9701547
  REMARK    Review article
REFERENCE   14 (bases 1 to 14804)
  AUTHORS   Elliott,C.E., Becker,B., Oehler,S., Castanon,M.J., Hauptmann,R. and
            Wiche,G.
  TITLE     Plectin transcript diversity: identification and tissue
            distribution of variants with distinct first coding exons and
            rodless isoforms
  JOURNAL   Genomics 42 (1), 115-125 (1997)
   PUBMED   9177781
REFERENCE   15 (bases 1 to 14804)
  AUTHORS   Chavanas,S., Pulkkinen,L., Gache,Y., Smith,F.J., McLean,W.H.,
            Uitto,J., Ortonne,J.P. and Meneguzzi,G.
  TITLE     A homozygous nonsense mutation in the PLEC1 gene in patients with
            epidermolysis bullosa simplex with muscular dystrophy
  JOURNAL   J Clin Invest 98 (10), 2196-2200 (1996)
   PUBMED   8941634
REFERENCE   16 (bases 1 to 14804)
  AUTHORS   Pulkkinen,L., Smith,F.J., Shimizu,H., Murata,S., Yaoita,H.,
            Hachisuka,H., Nishikawa,T., McLean,W.H. and Uitto,J.
  TITLE     Homozygous deletion mutations in the plectin gene (PLEC1) in
            patients with epidermolysis bullosa simplex associated with
            late-onset muscular dystrophy
  JOURNAL   Hum Mol Genet 5 (10), 1539-1546 (1996)
   PUBMED   8894687
REFERENCE   17 (bases 1 to 14804)
  AUTHORS   Smith,F.J., Eady,R.A., Leigh,I.M., McMillan,J.R., Rugg,E.L.,
            Kelsell,D.P., Bryant,S.P., Spurr,N.K., Geddes,J.F., Kirtschig,G.,
            Milana,G., de Bono,A.G., Owaribe,K., Wiche,G., Pulkkinen,L.,
            Uitto,J., McLean,W.H. and Lane,E.B.
  TITLE     Plectin deficiency results in muscular dystrophy with epidermolysis
            bullosa
  JOURNAL   Nat Genet 13 (4), 450-457 (1996)
   PUBMED   8696340
REFERENCE   18 (bases 1 to 14804)
  AUTHORS   McLean,W.H., Pulkkinen,L., Smith,F.J., Rugg,E.L., Lane,E.B.,
            Bullrich,F., Burgeson,R.E., Amano,S., Hudson,D.L., Owaribe,K.,
            McGrath,J.A., McMillan,J.R., Eady,R.A., Leigh,I.M., Christiano,A.M.
            and Uitto,J.
  TITLE     Loss of plectin causes epidermolysis bullosa with muscular
            dystrophy: cDNA cloning and genomic organization
  JOURNAL   Genes Dev 10 (14), 1724-1735 (1996)
   PUBMED   8698233
REFERENCE   19 (bases 1 to 14804)
  AUTHORS   Gache,Y., Chavanas,S., Lacour,J.P., Wiche,G., Owaribe,K.,
            Meneguzzi,G. and Ortonne,J.P.
  TITLE     Defective expression of plectin/HD1 in epidermolysis bullosa
            simplex with muscular dystrophy
  JOURNAL   J Clin Invest 97 (10), 2289-2298 (1996)
   PUBMED   8636409
REFERENCE   20 (bases 1 to 14804)
  AUTHORS   Liu,C.G., Maercker,C., Castanon,M.J., Hauptmann,R. and Wiche,G.
  TITLE     Human plectin: organization of the gene, sequence analysis, and
            chromosome localization (8q24)
  JOURNAL   Proc Natl Acad Sci U S A 93 (9), 4278-4283 (1996)
   PUBMED   8633055
REFERENCE   21 (bases 1 to 14804)
  AUTHORS   Pegoraro,E. and Hoffman,E.P.
  TITLE     Limb-Girdle Muscular Dystrophy Overview - RETIRED CHAPTER, FOR
            HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301582
REFERENCE   22 (bases 1 to 14804)
  AUTHORS   So,J.Y. and Teng,J.
  TITLE     Epidermolysis Bullosa Simplex
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301543
REFERENCE   23 (bases 1 to 14804)
  AUTHORS   Abicht,A., Muller,J.S. and Lochmuller,H.
  TITLE     Congenital Myasthenic Syndromes Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301347
REFERENCE   24 (bases 1 to 14804)
  AUTHORS   Lucky,A.W. and Gorell,E.
  TITLE     Epidermolysis Bullosa with Pyloric Atresia
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301336
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff in
            collaboration with Gunther Rezniczek, Gerhard Wiche. The reference
            sequence was derived from AC109322.16 and AY480051.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_201384.2.
            
            Summary: Plectin is a prominent member of an important family of
            structurally and in part functionally related proteins, termed
            plakins or cytolinkers, that are capable of interlinking different
            elements of the cytoskeleton. Plakins, with their multi-domain
            structure and enormous size, not only play crucial roles in
            maintaining cell and tissue integrity and orchestrating dynamic
            changes in cytoarchitecture and cell shape, but also serve as
            scaffolding platforms for the assembly, positioning, and regulation
            of signaling complexes (reviewed in PMID: 9701547, 11854008, and
            17499243). Plectin is expressed as several protein isoforms in a
            wide range of cell types and tissues from a single gene located on
            chromosome 8 in humans (PMID: 8633055, 8698233). Until 2010, this
            locus was named plectin 1 (symbol PLEC1 in human; Plec1 in mouse
            and rat) and the gene product had been referred to as
            'hemidesmosomal protein 1' or 'plectin 1, intermediate filament
            binding 500kDa'. These names were superseded by plectin. The
            plectin gene locus in mouse on chromosome 15 has been analyzed in
            detail (PMID: 10556294, 14559777), revealing a genomic exon-intron
            organization with well over 40 exons spanning over 62 kb and an
            unusual 5' transcript complexity of plectin isoforms. Eleven exons
            (1-1j) have been identified that alternatively splice directly into
            a common exon 2 which is the first exon to encode plectin's highly
            conserved actin binding domain (ABD). Three additional exons (-1,
            0a, and 0) splice into an alternative first coding exon (1c), and
            two additional exons (2alpha and 3alpha) are optionally spliced
            within the exons encoding the acting binding domain (exons 2-8).
            Analysis of the human locus has identified eight of the eleven
            alternative 5' exons found in mouse and rat (PMID: 14672974); exons
            1i, 1j and 1h have not been confirmed in human. Furthermore,
            isoforms lacking the central rod domain encoded by exon 31 have
            been detected in mouse (PMID:10556294), rat (PMID: 9177781), and
            human (PMID: 11441066, 10780662, 20052759). The short alternative
            amino-terminal sequences encoded by the different first exons
            direct the targeting of the various isoforms to distinct
            subcellular locations (PMID: 14559777). As the expression of
            specific plectin isoforms was found to be dependent on cell type
            (tissue) and stage of development (PMID: 10556294, 12542521,
            17389230) it appears that each cell type (tissue) contains a unique
            set (proportion and composition) of plectin isoforms, as if
            custom-made for specific requirements of the particular cells.
            Concordantly, individual isoforms were found to carry out distinct
            and specific functions (PMID: 14559777, 12542521, 18541706). In
            1996, a number of groups reported that patients suffering from
            epidermolysis bullosa simplex with muscular dystrophy (EBS-MD)
            lacked plectin expression in skin and muscle tissues due to defects
            in the plectin gene (PMID: 8698233, 8941634, 8636409, 8894687,
            8696340). Two other subtypes of plectin-related EBS have been
            described: EBS-pyloric atresia (PA) and EBS-Ogna. For reviews of
            plectin-related diseases see PMID: 15810881, 19945614. Mutations in
            the plectin gene related to human diseases should be named based on
            the position in NM_000445 (variant 1, isoform 1c), unless the
            mutation is located within one of the other alternative first
            exons, in which case the position in the respective Reference
            Sequence should be used. [provided by RefSeq, Aug 2011].
            
            Transcript Variant: This variant (11) differs in the 5' UTR and the
            5' coding region compared to variant 1. The resulting protein
            (isoform 1a) contains a distinct N-terminus compared to isoform 1c.
            Plectin isoform 1a has been found to associate specifically with
            hemidesmosome-like structures in keratinocytes (PMID: 12542521).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY480051.1, SRR14038191.3606295.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: PMID: 18541706, 17389230
            MANE Ensembl match                 :: ENST00000345136.8/
                                                  ENSP00000344848.3
            RefSeq Select criteria             :: based on manual assertion,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7                 AC109322.16        132298-132304
            8-14804             AY480051.1         1-14797
FEATURES             Location/Qualifiers
     source          1..14804
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.3"
     gene            1..14804
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /note="plectin"
                     /db_xref="GeneID:5339"
                     /db_xref="HGNC:HGNC:9069"
                     /db_xref="MIM:601282"
     exon            1..248
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     CDS             137..13780
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /note="isoform 1a is encoded by transcript variant 11;
                     plectin 1, intermediate filament binding protein 500kDa;
                     hemidesmosomal protein 1"
                     /codon_start=1
                     /product="plectin isoform 1a"
                     /protein_id="NP_958786.1"
                     /db_xref="CCDS:CCDS43775.1"
                     /db_xref="GeneID:5339"
                     /db_xref="HGNC:HGNC:9069"
                     /db_xref="MIM:601282"
                     /translation="MSQHQLRVPQPEGLGRKRTSSEDNLYLAVLRASEGKKDERDRVQ
                     KKTFTKWVNKHLIKAQRHISDLYEDLRDGHNLISLLEVLSGDSLPREKGRMRFHKLQN
                     VQIALDYLRHRQVKLVNIRNDDIADGNPKLTLGLIWTIILHFQISDIQVSGQSEDMTA
                     KEKLLLWSQRMVEGYQGLRCDNFTSSWRDGRLFNAIIHRHKPLLIDMNKVYRQTNLEN
                     LDQAFSVAERDLGVTRLLDPEDVDVPQPDEKSIITYVSSLYDAMPRVPDVQDGVRANE
                     LQLRWQEYRELVLLLLQWMRHHTAAFEERRFPSSFEEIEILWSQFLKFKEMELPAKEA
                     DKNRSKGIYQSLEGAVQAGQLKVPPGYHPLDVEKEWGKLHVAILEREKQLRSEFERLE
                     CLQRIVTKLQMEAGLCEEQLNQADALLQSDVRLLAAGKVPQRAGEVERDLDKADSMIR
                     LLFNDVQTLKDGRHPQGEQMYRRVYRLHERLVAIRTEYNLRLKAGVAAPATQVAQVTL
                     QSVQRRPELEDSTLRYLQDLLAWVEENQHRVDGAEWGVDLPSVEAQLGSHRGLHQSIE
                     EFRAKIERARSDEGQLSPATRGAYRDCLGRLDLQYAKLLNSSKARLRSLESLHSFVAA
                     ATKELMWLNEKEEEEVGFDWSDRNTNMTAKKESYSALMRELELKEKKIKELQNAGDRL
                     LREDHPARPTVESFQAALQTQWSWMLQLCCCIEAHLKENAAYFQFFSDVREAEGQLQK
                     LQEALRRKYSCDRSATVTRLEDLLQDAQDEKEQLNEYKGHLSGLAKRAKAVVQLKPRH
                     PAHPMRGRLPLLAVCDYKQVEVTVHKGDECQLVGPAQPSHWKVLSSSGSEAAVPSVCF
                     LVPPPNQEAQEAVTRLEAQHQALVTLWHQLHVDMKSLLAWQSLRRDVQLIRSWSLATF
                     RTLKPEEQRQALHSLELHYQAFLRDSQDAGGFGPEDRLMAEREYGSCSHHYQQLLQSL
                     EQGAQEESRCQRCISELKDIRLQLEACETRTVHRLRLPLDKEPARECAQRIAEQQKAQ
                     AEVEGLGKGVARLSAEAEKVLALPEPSPAAPTLRSELELTLGKLEQVRSLSAIYLEKL
                     KTISLVIRGTQGAEEVLRAHEEQLKEAQAVPATLPELEATKASLKKLRAQAEAQQPTF
                     DALRDELRGAQEVGERLQQRHGERDVEVERWRERVAQLLERWQAVLAQTDVRQRELEQ
                     LGRQLRYYRESADPLGAWLQDARRRQEQIQAMPLADSQAVREQLRQEQALLEEIERHG
                     EKVEECQRFAKQYINAIKDYELQLVTYKAQLEPVASPAKKPKVQSGSESVIQEYVDLR
                     THYSELTTLTSQYIKFISETLRRMEEEERLAEQQRAEERERLAEVEAALEKQRQLAEA
                     HAQAKAQAEREAKELQQRMQEEVVRREEAAVDAQQQKRSIQEELQQLRQSSEAEIQAK
                     ARQAEAAERSRLRIEEEIRVVRLQLEATERQRGGAEGELQALRARAEEAEAQKRQAQE
                     EAERLRRQVQDESQRKRQAEVELASRVKAEAEAAREKQRALQALEELRLQAEEAERRL
                     RQAEVERARQVQVALETAQRSAEAELQSKRASFAEKTAQLERSLQEEHVAVAQLREEA
                     ERRAQQQAEAERAREEAERELERWQLKANEALRLRLQAEEVAQQKSLAQAEAEKQKEE
                     AEREARRRGKAEEQAVRQRELAEQELEKQRQLAEGTAQQRLAAEQELIRLRAETEQGE
                     QQRQLLEEELARLQREAAAATQKRQELEAELAKVRAEMEVLLASKARAEEESRSTSEK
                     SKQRLEAEAGRFRELAEEAARLRALAEEAKRQRQLAEEDAARQRAEAERVLAEKLAAI
                     GEATRLKTEAEIALKEKEAENERLRRLAEDEAFQRRRLEEQAAQHKADIEERLAQLRK
                     ASDSELERQKGLVEDTLRQRRQVEEEILALKASFEKAAAGKAELELELGRIRSNAEDT
                     LRSKEQAELEAARQRQLAAEEERRRREAEERVQKSLAAEEEAARQRKAALEEVERLKA
                     KVEEARRLRERAEQESARQLQLAQEAAQKRLQAEEKAHAFAVQQKEQELQQTLQQEQS
                     VLDQLRGEAEAARRAAEEAEEARVQAEREAAQSRRQVEEAERLKQSAEEQAQARAQAQ
                     AAAEKLRKEAEQEAARRAQAEQAALRQKQAADAEMEKHKKFAEQTLRQKAQVEQELTT
                     LRLQLEETDHQKNLLDEELQRLKAEATEAARQRSQVEEELFSVRVQMEELSKLKARIE
                     AENRALILRDKDNTQRFLQEEAEKMKQVAEEAARLSVAAQEAARLRQLAEEDLAQQRA
                     LAEKMLKEKMQAVQEATRLKAEAELLQQQKELAQEQARRLQEDKEQMAQQLAEETQGF
                     QRTLEAERQRQLEMSAEAERLKLRVAEMSRAQARAEEDAQRFRKQAEEIGEKLHRTEL
                     ATQEKVTLVQTLEIQRQQSDHDAERLREAIAELEREKEKLQQEAKLLQLKSEEMQTVQ
                     QEQLLQETQALQQSFLSEKDSLLQRERFIEQEKAKLEQLFQDEVAKAQQLREEQQRQQ
                     QQMEQERQRLVASMEEARRRQHEAEEGVRRKQEELQQLEQQRRQQEELLAEENQRLRE
                     QLQLLEEQHRAALAHSEEVTASQVAATKTLPNGRDALDGPAAEAEPEHSFDGLRRKVS
                     AQRLQEAGILSAEELQRLAQGHTTVDELARREDVRHYLQGRSSIAGLLLKATNEKLSV
                     YAALQRQLLSPGTALILLEAQAASGFLLDPVRNRRLTVNEAVKEGVVGPELHHKLLSA
                     ERAVTGYKDPYTGQQISLFQAMQKGLIVREHGIRLLEAQIATGGVIDPVHSHRVPVDV
                     AYRRGYFDEEMNRVLADPSDDTKGFFDPNTHENLTYLQLLERCVEDPETGLCLLPLTD
                     KAAKGGELVYTDSEARDVFEKATVSAPFGKFQGKTVTIWEIINSEYFTAEQRRDLLRQ
                     FRTGRITVEKIIKIIITVVEEQEQKGRLCFEGLRSLVPAAELLESRVIDRELYQQLQR
                     GERSVRDVAEVDTVRRALRGANVIAGVWLEEAGQKLSIYNALKKDLLPSDMAVALLEA
                     QAGTGHIIDPATSARLTVDEAVRAGLVGPEFHEKLLSAEKAVTGYRDPYTGQSVSLFQ
                     ALKKGLIPREQGLRLLDAQLSTGGIVDPSKSHRVPLDVACARGCLDEETSRALSAPRA
                     DAKAYSDPSTGEPATYGELQQRCRPDQLTGLSLLPLSEKAARARQEELYSELQARETF
                     EKTPVEVPVGGFKGRTVTVWELISSEYFTAEQRQELLRQFRTGKVTVEKVIKILITIV
                     EEVETLRQERLSFSGLRAPVPASELLASGVLSRAQFEQLKDGKTTVKDLSELGSVRTL
                     LQGSGCLAGIYLEDTKEKVSIYEAMRRGLLRATTAALLLEAQAATGFLVDPVRNQRLY
                     VHEAVKAGVVGPELHEQLLSAEKAVTGYRDPYSGSTISLFQAMQKGLVLRQHGIRLLE
                     AQIATGGIIDPVHSHRVPVDVAYQRGYFSEEMNRVLADPSDDTKGFFDPNTHENLTYR
                     QLLERCVEDPETGLRLLPLKGAEKAEVVETTQVYTEEETRRAFEETQIDIPGGGSHGG
                     STMSLWEVMQSDLIPEEQRAQLMADFQAGRVTKERMIIIIIEIIEKTEIIRQQGLASY
                     DYVRRRLTAEDLFEARIISLETYNLLREGTRSLREALEAESAWCYLYGTGSVAGVYLP
                     GSRQTLSIYQALKKGLLSAEVARLLLEAQAATGFLLDPVKGERLTVDEAVRKGLVGPE
                     LHDRLLSAERAVTGYRDPYTEQTISLFQAMKKELIPTEEALRLLDAQLATGGIVDPRL
                     GFHLPLEVAYQRGYLNKDTHDQLSEPSEVRSYVDPSTDERLSYTQLLRRCRRDDGTGQ
                     LLLPLSDARKLTFRGLRKQITMEELVRSQVMDEATALQLREGLTSIEEVTKNLQKFLE
                     GTSCIAGVFVDATKERLSVYQAMKKGIIRPGTAFELLEAQAATGYVIDPIKGLKLTVE
                     EAVRMGIVGPEFKDKLLSAERAVTGYKDPYSGKLISLFQAMKKGLILKDHGIRLLEAQ
                     IATGGIIDPEESHRLPVEVAYKRGLFDEEMNEILTDPSDDTKGFFDPNTEENLTYLQL
                     MERCITDPQTGLCLLPLKEKKRERKTSSKSSVRKRRVVIVDPETGKEMSVYEAYRKGL
                     IDHQTYLELSEQECEWEEITISSSDGVVKSMIIDRRSGRQYDIDDAIAKNLIDRSALD
                     QYRAGTLSITEFADMLSGNAGGFRSRSSSVGSSSSYPISPAVSRTQLASWSDPTEETG
                     PVAGILDTETLEKVSITEAMHRNLVDNITGQRLLEAQACTGGIIDPSTGERFPVTDAV
                     NKGLVDKIMVDRINLAQKAFCGFEDPRTKTKMSAAQALKKGWLYYEAGQRFLEVQYLT
                     GGLIEPDTPGRVPLDEALQRGTVDARTAQKLRDVGAYSKYLTCPKTKLKISYKDALDR
                     SMVEEGTGLRLLEAAAQSTKGYYSPYSVSGSGSTAGSRTGSRTGSRAGSRRGSFDATG
                     SGFSMTFSSSSYSSSGYGRRYASGSSASLGGPESAVA"
     exon            249..310
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            311..400
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            401..478
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            479..571
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            572..738
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            739..854
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            855..961
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1081
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1082..1177
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..1305
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1306..1399
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1400..1554
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1555..1873
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1874..1951
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1952..2113
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2114..2218
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2219..2314
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2315..2440
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2441..2593
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2594..2748
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2749..2875
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2876..3059
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3060..3217
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3218..3396
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3397..3535
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3536..3892
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3893..3976
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3977..4081
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            4082..4180
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            4181..7561
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            7562..14804
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      14784..14789
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /note="hexamer: AATAAA"
     polyA_site      14804
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /note="major polyA site"
ORIGIN      
        1 ggggctctga gctgagcctg tgccgagtga gcgtctcgtg gggccgccag ccttcagctg
       61 cctgtgtgcc acacggggct gcccagagca ccgtgcctga ggccgagggt ccgggcgacg
      121 aaggtgtggg ggcagcatgt ctcagcacca gctccgcgtg ccgcagcccg agggcctggg
      181 ccgaaagaga accagctcgg aggacaacct gtacctggct gtgctcaggg cctctgaggg
      241 caagaaagat gagcgggatc gtgtgcagaa gaaaaccttc accaagtggg tcaacaagca
      301 cctcatcaag gcccagaggc acatcagtga cctgtatgaa gacctccgcg atggccacaa
      361 cctcatctcc ctgctggagg tcctctcggg ggacagcctg ccccgggaga aggggaggat
      421 gcgtttccac aagctgcaga atgtccagat tgccctggac tacctccggc accgccaggt
      481 gaagctggtg aacatcagga atgatgacat cgctgacggc aaccccaagc tgacccttgg
      541 cctcatctgg acaatcattc tgcacttcca gatctcagat atccaggtga gtgggcagtc
      601 ggaggacatg acggccaagg agaagctgct gctgtggtcg cagcgaatgg tggaggggta
      661 ccagggcctg cgatgcgaca acttcacctc cagctggaga gacggccgcc tcttcaatgc
      721 catcatccac cggcacaagc ccctgctcat cgacatgaac aaggtgtacc ggcagaccaa
      781 cctggagaac ctggaccagg ccttctctgt ggcggagcgg gacctgggag tgacgcggct
      841 cctggaccct gaggacgtgg atgtccctca gcccgacgag aagtccatca tcacctacgt
      901 ctcgtcgctg tatgacgcca tgccccgcgt gccggacgtg caggatgggg tgagggccaa
      961 cgagctgcag ctgcgctggc aggagtaccg ggagctggtg ctgctgctgc ttcagtggat
     1021 gcgacaccac acggccgcct ttgaggaacg caggttcccc tccagcttcg aggagattga
     1081 gatcctgtgg tctcagttcc tgaagtttaa ggagatggag ctaccagcca aggaggccga
     1141 caagaacagg tccaagggca tctaccaatc cctggaggga gcggtgcaag caggccagct
     1201 caaggtgccc cctggctacc acccgctgga tgtggagaag gagtggggca agctgcacgt
     1261 ggccatcctg gagcgggaga agcagctccg cagcgagttt gagaggctgg agtgtcttca
     1321 gcgcatcgtg accaagctgc agatggaggc ggggctgtgt gaggagcagc tgaaccaggc
     1381 cgacgccctg ctgcagtcgg atgtccggct gctggctgca ggcaaagtgc cacagcgggc
     1441 gggggaggtg gaacgggact tggacaaggc ggatagcatg atccggctgc tcttcaacga
     1501 cgtgcagacc ctcaaggatg gacggcaccc gcagggcgag cagatgtacc gcagggtgta
     1561 ccgtctgcac gagcgcctgg tagccatccg caccgagtac aacctacggc tgaaggcagg
     1621 cgtggcggcc cctgcaaccc aggtggccca ggtgactctg cagagtgtgc agaggcgccc
     1681 cgagctggag gactccactc tgcgctacct gcaggacctg ctggcctggg tggaggagaa
     1741 ccagcaccgt gtggatggcg ctgagtgggg tgtggacctg cccagcgtgg aggcgcagct
     1801 gggcagccac cgaggcctgc accagtccat cgaagaattc cgggccaaga tcgagcgggc
     1861 acggagtgac gagggccagc tctcccccgc cacccggggt gcctaccgtg actgcctggg
     1921 tcggctggac ctgcagtacg ccaagctgct gaactcctcc aaggcccgcc tcaggtccct
     1981 ggagagcttg cacagctttg tggcagccgc cactaaggag ctaatgtggc tgaatgagaa
     2041 ggaggaggag gaggtgggct tcgactggag cgaccgcaac accaacatga ccgccaagaa
     2101 ggagagctac tcggcgctga tgcgggagct ggagctgaag gagaagaaga tcaaggagct
     2161 ccaaaatgct ggggaccggc tgctgcggga ggaccacccg gcccggccca cggtggagtc
     2221 cttccaggcg gccctgcaga cgcagtggag ctggatgcta cagctgtgct gctgtatcga
     2281 ggcacacctg aaggagaacg ctgcctactt tcagttcttc tcagatgtgc gggaggccga
     2341 ggggcagttg cagaagctgc aggaggcact gcgtaggaaa tacagttgtg atcgctccgc
     2401 caccgtcacc cggctggagg acctgctgca ggatgcccag gacgagaagg aacagctgaa
     2461 cgagtacaag ggccacctct caggcctggc caagcgggcc aaggccgtcg tgcagctgaa
     2521 gccccgccac ccagcccacc ccatgcgggg ccgcctgccc ctgctggccg tgtgcgacta
     2581 taagcaggtg gaggtgactg tgcacaaggg tgacgagtgc cagctggtgg gccctgcaca
     2641 gccgtcccac tggaaggtgc tcagcagctc cggcagcgag gccgccgtgc cctccgtgtg
     2701 cttcctggtg cccccgccca accaggaggc ccaggaggcc gtcaccaggc tggaggccca
     2761 gcaccaggcc ctggtcacgc tgtggcacca gttgcacgtg gacatgaaga gccttctggc
     2821 ctggcagagc cttcgccgcg acgtgcagct catccgctcc tggtccctgg ccacgttccg
     2881 caccctgaag ccagaggagc agcgccaagc cctgcacagc ctggagctgc actaccaggc
     2941 cttcctgcgg gacagccagg acgcgggcgg cttcggaccc gaggaccggc tgatggctga
     3001 gcgcgagtac ggctcctgca gccaccacta ccagcagctg ctgcagagcc tggaacaggg
     3061 tgcacaggaa gagtctcgct gccagcgctg catctccgag ctcaaagaca tccggctgca
     3121 gctggaggcc tgtgagacgc gcaccgtgca ccgcctgcgg ctgccgctgg acaaagagcc
     3181 ggcacgggag tgtgcccagc gcatcgccga gcagcagaag gcacaggcag aggtggaggg
     3241 gctgggcaag ggggtcgccc ggctctctgc cgaggccgag aaggtcttgg ccctaccaga
     3301 gccatcgcct gcggccccca cgctgcgctc ggagctggag ctgacgctgg gcaagctgga
     3361 gcaggtccgc agcctgtctg ccatctacct ggagaagctc aagaccatca gcctggtgat
     3421 ccgcggcacg cagggggccg aggaggtgct cagggcccac gaggagcagc tcaaggaggc
     3481 ccaggccgtg ccggccaccc tcccggagct cgaggccacc aaggcctctc tgaagaagct
     3541 gcgggcccag gccgaggcac agcagcccac gttcgacgcc ctgcgggatg agctgcgggg
     3601 ggcacaggag gtgggggagc gactgcagca gcggcacggg gagcgggacg tggaggtgga
     3661 gcgctggcgg gagcgggtcg cccagttgct tgagcgctgg caggctgtgc tggcccagac
     3721 cgacgtgcgg cagcgcgagc tcgagcaact gggccgccag ctgcgttact accgcgagag
     3781 tgcagacccc ttgggcgcct ggctgcagga cgccaggcgg cggcaggagc agatccaggc
     3841 catgccgctg gccgacagcc aggctgtgcg ggagcagctg cggcaggagc aggccctgct
     3901 ggaggagatc gagcgccacg gcgagaaggt cgaggagtgc cagaggtttg cgaaacagta
     3961 catcaacgcc atcaaggact atgaactcca gctggtgacg tacaaggcgc agcttgagcc
     4021 ggtggcctcc ccggccaaga agcccaaggt ccagtcggga tcagagagtg tcatccagga
     4081 gtacgtggac ctgcgtacgc actacagcga gctgaccaca ctgacgagcc agtacatcaa
     4141 gttcatcagc gagactctgc ggcgcatgga ggaggaggag aggctggctg agcagcagcg
     4201 ggcagaggag cgcgagcggc tggccgaggt ggaggccgcg ctggagaagc agcggcagct
     4261 ggccgaggcg cacgcccagg caaaggcaca ggcggagcgg gaggcgaagg agctgcagca
     4321 gcgcatgcag gaggaggtgg tgcggcggga ggaggcggcg gtggacgcgc agcagcagaa
     4381 gcgcagcatt caggaggagc tgcagcagct gcggcagagc tcggaggcgg agatccaggc
     4441 caaggcccgg caggcagagg cggctgagcg cagccggctg cgcatcgagg aggagatccg
     4501 cgtggtgcgc ctgcagttgg aggccaccga gcgccagcgt ggcggggctg agggggagct
     4561 gcaggcactg cgtgcacggg cggaggaggc tgaggcacaa aagcgacagg cgcaggagga
     4621 ggccgagcgc ttgcggaggc aggtgcagga cgagagccag cgtaagcggc aggcggaggt
     4681 ggagctggcc tcgcgcgtga aggccgaggc cgaggcggcg cgcgagaagc agcgggccct
     4741 gcaggccctg gaggagctgc ggctgcaggc ggaggaggcg gagcggcgcc tgcggcaggc
     4801 cgaggtggag cgagcgcggc aggtacaggt ggccctggag acggcgcagc gcagtgcaga
     4861 ggcggagctg cagagcaaac gcgcctcctt cgccgagaag acggcacagc tggagcgctc
     4921 cctgcaggag gaacacgtgg ctgtggcaca gctgcgggag gaggctgagc ggcgggcaca
     4981 gcagcaggcc gaggccgagc gggcgcgcga ggaggcagag cgggagctgg agcgctggca
     5041 gctcaaggcc aacgaggcgc tacggctgcg gctgcaggcg gaggaggtgg cgcagcagaa
     5101 gagcctggcg caggccgagg ctgagaagca gaaggaggag gcggagcgcg aggcgcggcg
     5161 gcgcggcaag gcggaggagc aggccgtccg gcagcgggag ctggctgaac aagagctgga
     5221 gaagcagcgg cagctggcgg aaggcaccgc gcagcagcgc ctggccgcgg agcaggagtt
     5281 gatccggctg cgggccgaga cggagcaggg ggagcagcag cggcagctgc tggaggagga
     5341 gctggcccgg ctgcagcgtg aggcggctgc agccacgcag aaacggcagg agctggaagc
     5401 cgagctggcc aaggtgcggg ccgagatgga ggtgctgctg gccagcaagg cgagggctga
     5461 ggaggagtcg cgctccacca gcgagaagtc caagcagagg ctggaggccg aggccggccg
     5521 gttccgcgag ctggccgagg aggccgcccg cctgcgtgcc ctggcggaag aggccaagcg
     5581 gcagcggcag ctggccgagg aagacgcggc gcggcagcgg gccgaggcgg agcgggtgct
     5641 tgcggagaag ctggccgcca tcggcgaggc cacgcggctc aagacggagg cggagatcgc
     5701 gctcaaggag aaggaggcgg agaacgagcg cctgcggcgg ctggcggagg acgaggcctt
     5761 ccagcggcgg cggctggagg agcaggccgc gcaacacaag gctgacatcg aggagcgcct
     5821 ggcccagctg cgcaaggcat cggacagcga gctggagcgg cagaaggggc tggtggagga
     5881 cacgctgagg cagcggcggc aggtggagga ggagatcctg gcgctgaagg cgagcttcga
     5941 gaaggcggcc gctggcaagg cggagctgga gctggagctg ggacgcatcc gcagcaacgc
     6001 ggaggacacg ctgcgcagca aggagcaggc cgagctggag gctgcgaggc agcggcagct
     6061 ggcggcggag gaggagcggc ggcgccgtga ggctgaggag cgcgtgcaga agagcctggc
     6121 ggccgaggag gaggccgcac ggcagcggaa ggcggcgctg gaggaagtcg agcggctgaa
     6181 agccaaggtg gaggaggcgc ggcgcctgcg ggagcgagcg gagcaggagt cggcgcggca
     6241 gctgcagctg gcccaggagg ccgcccagaa gcggctgcag gcggaagaga aggcacacgc
     6301 cttcgcggtg cagcagaagg agcaggagct acagcagacg ctgcagcagg agcagagcgt
     6361 gctggaccag ctgcgcggcg aggcggaggc ggcccggcgg gcggctgagg aggcggagga
     6421 ggcccgggtg caggcggagc gtgaggcggc gcagtcccgg cggcaggtgg aagaggccga
     6481 gcggctgaag cagtcggcag aggagcaggc acaggcccgg gctcaggcac aggcggctgc
     6541 agagaagctg cgcaaggagg ccgagcaaga ggcggcgcgg cgggcacagg cggagcaggc
     6601 ggccctgcgg cagaagcagg cagctgacgc ggagatggag aagcataaga aattcgccga
     6661 gcagacgctg cggcagaagg cgcaggtgga gcaggagctg acaacactgc ggctgcagct
     6721 ggaggagacc gaccaccaga agaacctgct ggacgaggag ctgcagcggc tgaaggcgga
     6781 ggccacggag gccgcacgcc agcgcagcca ggtggaggag gagctcttct cggtgcgcgt
     6841 gcagatggag gagctgagca agctcaaggc acgcatcgag gctgagaacc gcgcactcat
     6901 cttgcgtgac aaggacaata cgcagcgctt cctgcaggag gaggctgaga agatgaagca
     6961 ggtggcggag gaggccgcgc ggctgagtgt ggcggcccaa gaggctgcgc gactgcggca
     7021 gctggcagag gaggacctgg cacagcagcg ggccttggca gagaagatgc tcaaggagaa
     7081 gatgcaggcg gtgcaggagg ccacgcgact caaggctgag gcggaactgc tgcagcagca
     7141 gaaggagctt gcgcaggagc aggcgcggcg gctgcaggag gacaaggagc agatggcgca
     7201 gcagctggcg gaggagacgc agggcttcca gcggacgctg gaggccgagc ggcagcggca
     7261 gctggagatg agcgctgagg ctgagcgcct caagctgcgt gtggccgaga tgagccgagc
     7321 ccaggcccgc gctgaggagg acgcccagcg cttccggaag caggcggagg agatcggtga
     7381 gaagctgcac cgcacggagc tcgccaccca ggagaaggtg accctggtgc agacactgga
     7441 gatccagcga cagcagagtg accatgatgc cgagcgcctg cgggaggcca tcgctgagct
     7501 ggagcgtgag aaggagaagc tccaacagga ggccaaactg ctgcagctca agtctgagga
     7561 gatgcagacg gtgcagcagg agcagctgct gcaggagacg caggccctgc agcaaagctt
     7621 cctctctgaa aaggacagcc tgctacagcg ggagcgcttc atcgagcagg agaaggccaa
     7681 gctggagcag ctcttccagg acgaggtggc caaggcacag cagctgcgtg aggagcagca
     7741 gcggcagcag cagcagatgg agcaggaacg gcagcggctg gtggccagca tggaggaggc
     7801 gcggcggcgg cagcatgagg ccgaggaggg cgtgcggcgc aagcaggagg agctgcagca
     7861 gctggagcag cagcggcggc agcaggagga gctgctggct gaggagaacc agaggctgcg
     7921 tgagcagctg cagctcctgg aggagcagca ccgggccgcg ctggcgcact cagaggaggt
     7981 cactgcctcg caggtggctg ccacaaagac cctgcccaat ggccgggatg cacttgatgg
     8041 ccccgcggca gaggcagagc cggagcacag cttcgatggc ctgcggcgga aggtgtcagc
     8101 tcagaggctg caggaggccg gcatcctgag tgcggaggag ctgcagcggt tggcgcaggg
     8161 ccacaccacg gtggacgagc tcgcacggcg ggaagacgtg cgccactacc tgcagggccg
     8221 cagcagtatc gcagggctgt tgctgaaggc caccaatgag aagctgagtg tttacgccgc
     8281 cctgcagagg cagctgctga gtcccggcac ggccctcatc ctgctggagg cgcaggcggc
     8341 ctcaggcttc ctgctggacc ctgtgcggaa ccggcggctg accgtcaacg aggctgtgaa
     8401 ggagggtgtg gtgggccccg agctgcacca caagctgctg tcggccgagc gcgccgtcac
     8461 tggctacaag gacccctaca ctggccagca gatctctctc ttccaagcca tgcagaaggg
     8521 cctcatcgtc cgggagcacg gcatccgcct gctggaggcc cagatcgcca cgggcggcgt
     8581 tatcgacccc gtgcacagcc accgcgtgcc cgtggacgtg gcctaccggc gcggctactt
     8641 cgacgaggag atgaaccgcg tcctggcgga ccccagcgac gacaccaagg gcttctttga
     8701 ccccaacacg cacgagaacc tcacgtacct gcagctactg gagcgctgcg tggaggaccc
     8761 cgagacgggc ctgtgccttc tgccactcac ggataaggct gccaagggcg gggagctggt
     8821 ctacactgac tccgaggccc gggacgtctt tgagaaggcc accgtgtctg cgccgttcgg
     8881 caagttccag ggcaagacgg tgaccatttg ggagatcatc aactcggaat acttcacggc
     8941 agagcagcgg cgggacctgc tgcggcagtt ccgcacgggc cggatcacag tggagaagat
     9001 catcaagatc atcatcacgg tggtggagga gcaggagcag aagggccggc tttgctttga
     9061 gggcctgcgc agcctggtgc cagccgccga gctgctggag agcagggtca tcgaccgcga
     9121 gctctaccag cagctgcagc gaggtgagcg ctctgtgcga gacgtagccg aggtggacac
     9181 tgtgcggcgg gctctccggg gtgccaacgt catcgcgggt gtatggctgg aggaggcggg
     9241 gcagaagctg agtatctaca atgccctgaa gaaagacctg ctgccatccg acatggccgt
     9301 ggccctgttg gaagcccagg ccggcaccgg gcacatcatc gaccccgcca ccagcgcccg
     9361 gctgaccgtg gacgaggcag tgcgtgctgg cctggtgggc cccgagtttc atgagaagct
     9421 gctatcagcc gagaaggctg tgacagggta cagggacccc tacacagggc agagcgtctc
     9481 cctgttccag gccctgaaga agggcctcat tccccgggag cagggcctgc gcctgttgga
     9541 cgcccagctg tccacgggcg gcatcgtgga ccccagcaag agccaccgcg tgcccctgga
     9601 tgtcgcctgc gcccgaggct gcctggatga ggagaccagc agggccctgt cggcaccaag
     9661 ggccgacgcc aaggcctaca gtgaccccag cacaggggag ccggccacct acggcgagct
     9721 ccagcagcgg tgccggcccg accagctgac cgggctgagc ctgctgccgc tctcagaaaa
     9781 ggctgctcgg gcccggcagg aggagctcta ctcagagctg caggcccgtg agacctttga
     9841 aaagaccccg gttgaggtcc ccgtgggtgg cttcaagggc aggacggtga cggtgtggga
     9901 gctcatcagc tctgagtact tcactgcgga gcagcggcag gagctgttgc gtcagttccg
     9961 cacgggcaag gtcaccgtgg agaaggtcat caagattctc attaccatcg tggaggaggt
    10021 ggagaccctg cggcaggaga ggctgtcctt cagcggcctc cgtgcccctg tgccagccag
    10081 cgagctcctg gcttccgggg tcctcagcag agcccagttt gagcagctca aggacggcaa
    10141 gacgacggtc aaggaccttt cggagctggg ctccgtgcgg acgctgctgc agggcagtgg
    10201 ctgcctcgcc ggcatctacc tggaggacac caaggagaag gtgtccatct acgaggccat
    10261 gcgccggggc ctgctgagag ccacaacggc tgcgctcctg ctggaggcgc aggcggccac
    10321 tggcttcctg gtggaccccg tgcggaacca gcgcctgtat gtccacgagg ccgtgaaggc
    10381 gggcgtggtg ggccccgagc ttcacgagca gctgctgtct gccgagaagg ccgtcaccgg
    10441 ctacagagac ccctactcgg gcagcaccat ctccctcttc caggccatgc agaagggcct
    10501 ggttctccgg cagcacggca tccgcctgct ggaggcccag atcgccacgg gcggcatcat
    10561 cgaccccgtg cacagccacc gcgtgcctgt ggacgtggcc taccagcgcg gctacttcag
    10621 tgaggagatg aaccgcgtcc tggcggaccc cagcgacgac accaagggct tctttgaccc
    10681 caacacgcat gagaacctca cgtacaggca gctgctggag cggtgcgtgg aggaccccga
    10741 gacgggcttg cgccttctgc cactgaaagg ggcggagaag gctgaggtgg tggagaccac
    10801 gcaggtgtac actgaggagg agacaagaag ggcatttgaa gagacacaga tcgacattcc
    10861 cggcggcggc agccacggcg gctccaccat gtccctgtgg gaggtgatgc agtcggacct
    10921 gatccccgag gagcagcggg cccagctgat ggctgacttc caggccggcc gggtgaccaa
    10981 ggaacgcatg atcatcatca tcatcgagat cattgagaag acagagatca tccgccagca
    11041 gggtctggcc tcctacgact acgtgcgccg ccgcctcacg gctgaggacc tgttcgaggc
    11101 tcggatcatc tctctcgaga cctacaacct gctccgggag ggcaccagga gcctccgtga
    11161 ggctctcgag gcggagtccg cctggtgcta cctctatggc acgggctccg tggctggtgt
    11221 ctacctgccc ggttccaggc agacactgag catctaccag gctctcaaga aagggctgct
    11281 gagtgccgag gtggcccgcc tgctgctgga ggcacaggca gccacaggct tcctgctgga
    11341 cccggtgaag ggggagcggc tgactgtgga tgaggctgtg cggaagggcc tcgtggggcc
    11401 cgagctgcac gaccgcctgc tctcggctga gcgggcggtc accggctacc gtgaccccta
    11461 caccgagcag accatctcgc tcttccaggc catgaagaag gagctgatcc ctactgagga
    11521 ggccctgcgg ctgctggatg cccagctggc caccggcggc atcgtggacc cccgcctggg
    11581 cttccacctt cccctggagg tggcttacca gcgtggctac ctcaacaagg acacgcacga
    11641 ccagctgtca gagcccagcg aggtgcgcag ctacgtggac ccgtccaccg acgagcgcct
    11701 cagctacacg cagctgctca ggcggtgccg tcgtgacgac ggcaccggcc agctgctcct
    11761 gccactgtcg gacgcccgca agctgacctt ccgtggcctg cggaagcaga tcaccatgga
    11821 ggagctggtg cgctcgcagg tcatggacga ggccacggcg ctgcagctgc gggagggcct
    11881 gacctccatc gaggaggtca ccaagaactt gcagaagttc ctggaaggca ccagctgcat
    11941 cgctggtgtc ttcgtggacg ccaccaagga acggctctcg gtgtaccagg ccatgaagaa
    12001 gggcatcatc cgccccggca cagcctttga gctcctggag gcgcaggcgg ccaccggtta
    12061 cgtcatcgac cccatcaagg gactgaagct gacggtggag gaggctgtgc gtatgggcat
    12121 tgtgggcccc gagttcaagg acaagctgct gtcggccgag cgcgccgtca ctgggtacaa
    12181 ggacccctac tctgggaagc tcatctccct cttccaggcc atgaagaagg gcctgatcct
    12241 gaaggaccat ggcatccgcc tgctggaggc ccagatcgcc acgggcggca tcatcgaccc
    12301 tgaggagagc caccggctgc ccgtggaggt ggcctacaag cgcggcctct tcgatgagga
    12361 gatgaacgag atcctgaccg acccctcgga cgacaccaag ggcttctttg accctaacac
    12421 ggaggagaac ctcacctacc tgcagctgat ggagcgttgt atcactgacc cccagacggg
    12481 cctgtgtctc ttgccgctga aggagaagaa gcgggagcgg aagacgtcct ccaagtcctc
    12541 cgtgcgcaag cgccgagtgg tcatcgtgga ccccgagacg ggcaaggaga tgtcagtgta
    12601 cgaggcctac cgcaagggcc tgattgacca ccagacgtac ctggagctgt ccgagcagga
    12661 gtgcgagtgg gaggagatca ccatctcctc ctcggacggc gtggtcaagt ccatgatcat
    12721 cgaccgccgc tccgggcgcc agtacgacat cgatgatgcc atcgccaaga acctcatcga
    12781 ccgctcggca ctggaccagt accgcgccgg cacgctctcc atcaccgagt tcgccgacat
    12841 gctctcgggc aacgccggtg gtttccgctc ccgttcctcc tcggtgggat cctcctcctc
    12901 ctaccccatc agccccgccg tctccaggac ccagctggcc tcctggtcag accccactga
    12961 ggagacgggc cccgtggctg gcatcctgga cacggagacg ctggagaagg tgtccatcac
    13021 cgaggccatg caccggaacc tggtggataa catcacgggg cagcggctgc tggaggcgca
    13081 ggcctgcacc gggggcatca tcgaccccag caccggtgag cgcttccctg tcaccgacgc
    13141 cgtcaacaag ggcctggtgg acaagatcat ggtggaccgc atcaacctgg cccagaaggc
    13201 cttctgcggc ttcgaggacc cacgcaccaa gaccaagatg tcggccgccc aggccctgaa
    13261 gaagggctgg ctctactacg aggccggcca gcgcttcctg gaggtgcagt acctgaccgg
    13321 cggcttgatc gagcccgaca cgccgggccg cgtgcccctg gacgaggccc tgcagcgcgg
    13381 cacggtggac gcccgcaccg cacagaagct gcgtgacgtg ggcgcctact ccaagtacct
    13441 cacctgccct aagaccaagc tcaagatctc ctataaggac gcgctggacc gcagcatggt
    13501 ggaggagggc acggggctgc ggctgctgga ggctgccgcg cagtccacca agggctacta
    13561 cagcccctac agcgtcagcg gctccggctc taccgctggc tcccgcaccg gctcgcgcac
    13621 cggctcccgg gccggctccc gccgcggcag ctttgacgcc accggctccg gcttctccat
    13681 gaccttctct tcatcctcct actcctcctc gggctacggc cgccgctacg cctcggggtc
    13741 ctcggcctcc ctggggggcc ctgagtctgc cgtggcctga ggctgcctgc gcccaccccg
    13801 ctctgcatgc ggcccagccc ggctcccacc gaggcgcggg ggccgttttc aacgcttaaa
    13861 ggtgtcttcc tcccaagtgg tgcctaaagt ttaaccaaaa agaccagact aatatattaa
    13921 tatatatctg ctgtccagac agcctgtatc ttgggggaca gggctggccc agccctgctg
    13981 gccgcctcac cccctcgggt ctcctcactc ccttctacct gccactcaca cagccaggtg
    14041 ccttggaggg tcccaagctg ggccccagcc caccctcctg tcttcccagg gtagcccgcc
    14101 tgccagtcct agctgcacag ggcagctggg cccaaccctg tctgtagagg gccctggtgt
    14161 ttctagcact ggcctgcacg gtgggccttg ctggggacgg ggggccccag tcagcctctc
    14221 tcccagtcta cccagagaag ccccttcccc atgggaagac gaggccctcg ggcccagccc
    14281 ccacagtgct gtctgatctg tgctttccag ctcacccccc acactcactc ctgagacccc
    14341 tggcctccgg cgtcagcctc cagcctctgt tcccctagta agtgccttcc atgtcggcct
    14401 ctaaccccag gccccgagga cccagaccca gtggggaggc ggacgttcca gccggcatgg
    14461 ctgggaactg cagacctgtc ctcctggtgg gtccaggggc ccctccagct tgtggagccc
    14521 cacactgggg tgccgcctgc ccgtctctct cccatggagc cccagccccc tttgggccca
    14581 gggacaccag ccaggctctg tgctgaccct cctgttgcac ccagccctgg tctcagcagc
    14641 gaccacccct gcctccaccc tctgagcttt gcatgttcca ctaaccccgg gcgggtggca
    14701 ggtggaggtg tcaggctgct ggcgcctctg caagggcaga acactaacct gaccgtgggc
    14761 ggggccttgc ggtatccgcc cccaataaaa gcaattccaa cctt
//
