LOCUS       NM_201382              14763 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens plectin (PLEC), transcript variant 8, mRNA.
ACCESSION   NM_201382
VERSION     NM_201382.4
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 14763)
  AUTHORS   Rezniczek,G.A., Walko,G. and Wiche,G.
  TITLE     Plectin gene defects lead to various forms of epidermolysis bullosa
            simplex
  JOURNAL   Dermatol Clin 28 (1), 33-41 (2010)
   PUBMED   19945614
  REMARK    Review article
            Erratum:[Dermatol Clin. 2010 Apr;28(2):439-41]
REFERENCE   2  (bases 1 to 14763)
  AUTHORS   Winter,L., Abrahamsberg,C. and Wiche,G.
  TITLE     Plectin isoform 1b mediates mitochondrion-intermediate filament
            network linkage and controls organelle shape
  JOURNAL   J Cell Biol 181 (6), 903-911 (2008)
   PUBMED   18541706
REFERENCE   3  (bases 1 to 14763)
  AUTHORS   Sonnenberg,A. and Liem,R.K.
  TITLE     Plakins in development and disease
  JOURNAL   Exp Cell Res 313 (10), 2189-2203 (2007)
   PUBMED   17499243
  REMARK    Review article
REFERENCE   4  (bases 1 to 14763)
  AUTHORS   Rezniczek,G.A., Konieczny,P., Nikolic,B., Reipert,S., Schneller,D.,
            Abrahamsberg,C., Davies,K.E., Winder,S.J. and Wiche,G.
  TITLE     Plectin 1f scaffolding at the sarcolemma of dystrophic (mdx) muscle
            fibers through multiple interactions with beta-dystroglycan
  JOURNAL   J Cell Biol 176 (7), 965-977 (2007)
   PUBMED   17389230
REFERENCE   5  (bases 1 to 14763)
  AUTHORS   Pfendner,E., Rouan,F. and Uitto,J.
  TITLE     Progress in epidermolysis bullosa: the phenotypic spectrum of
            plectin mutations
  JOURNAL   Exp Dermatol 14 (4), 241-249 (2005)
   PUBMED   15810881
  REMARK    GeneRIF: discussion of phenotypic spectrum of plectin mutations
            [review]
            Review article
REFERENCE   6  (bases 1 to 14763)
  AUTHORS   Zhang,T., Haws,P. and Wu,Q.
  TITLE     Multiple variable first exons: a mechanism for cell- and
            tissue-specific gene regulation
  JOURNAL   Genome Res 14 (1), 79-89 (2004)
   PUBMED   14672974
REFERENCE   7  (bases 1 to 14763)
  AUTHORS   Rezniczek,G.A., Abrahamsberg,C., Fuchs,P., Spazierer,D. and
            Wiche,G.
  TITLE     Plectin 5'-transcript diversity: short alternative sequences
            determine stability of gene products, initiation of translation and
            subcellular localization of isoforms
  JOURNAL   Hum Mol Genet 12 (23), 3181-3194 (2003)
   PUBMED   14559777
REFERENCE   8  (bases 1 to 14763)
  AUTHORS   Andra,K., Kornacker,I., Jorgl,A., Zorer,M., Spazierer,D., Fuchs,P.,
            Fischer,I. and Wiche,G.
  TITLE     Plectin-isoform-specific rescue of hemidesmosomal defects in
            plectin (-/-) keratinocytes
  JOURNAL   J Invest Dermatol 120 (2), 189-197 (2003)
   PUBMED   12542521
REFERENCE   9  (bases 1 to 14763)
  AUTHORS   Leung,C.L., Green,K.J. and Liem,R.K.
  TITLE     Plakins: a family of versatile cytolinker proteins
  JOURNAL   Trends Cell Biol 12 (1), 37-45 (2002)
   PUBMED   11854008
  REMARK    Review article
REFERENCE   10 (bases 1 to 14763)
  AUTHORS   Brown,M.J., Hallam,J.A., Liu,Y., Yamada,K.M. and Shaw,S.
  TITLE     Cutting edge: integration of human T lymphocyte cytoskeleton by the
            cytolinker plectin
  JOURNAL   J Immunol 167 (2), 641-645 (2001)
   PUBMED   11441066
  REMARK    GeneRIF: During polarization of peripheral blood T lymphocytes,
            plectin redistributes to the uropod associated with vimentin and
            fodrin. This vimentin-plectin-fodrin complex provides a continuous
            linkage from the nucleus (lamin B) to the cortical cytoskeleton.
REFERENCE   11 (bases 1 to 14763)
  AUTHORS   Schroder,R., Furst,D.O., Klasen,C., Reimann,J., Herrmann,H. and van
            der Ven,P.F.
  TITLE     Association of plectin with Z-discs is a prerequisite for the
            formation of the intermyofibrillar desmin cytoskeleton
  JOURNAL   Lab Invest 80 (4), 455-464 (2000)
   PUBMED   10780662
REFERENCE   12 (bases 1 to 14763)
  AUTHORS   Fuchs,P., Zorer,M., Rezniczek,G.A., Spazierer,D., Oehler,S.,
            Castanon,M.J., Hauptmann,R. and Wiche,G.
  TITLE     Unusual 5' transcript complexity of plectin isoforms: novel
            tissue-specific exons modulate actin binding activity
  JOURNAL   Hum Mol Genet 8 (13), 2461-2472 (1999)
   PUBMED   10556294
REFERENCE   13 (bases 1 to 14763)
  AUTHORS   Wiche,G.
  TITLE     Role of plectin in cytoskeleton organization and dynamics
  JOURNAL   J Cell Sci 111 (Pt 17), 2477-2486 (1998)
   PUBMED   9701547
  REMARK    Review article
REFERENCE   14 (bases 1 to 14763)
  AUTHORS   Elliott,C.E., Becker,B., Oehler,S., Castanon,M.J., Hauptmann,R. and
            Wiche,G.
  TITLE     Plectin transcript diversity: identification and tissue
            distribution of variants with distinct first coding exons and
            rodless isoforms
  JOURNAL   Genomics 42 (1), 115-125 (1997)
   PUBMED   9177781
REFERENCE   15 (bases 1 to 14763)
  AUTHORS   Chavanas,S., Pulkkinen,L., Gache,Y., Smith,F.J., McLean,W.H.,
            Uitto,J., Ortonne,J.P. and Meneguzzi,G.
  TITLE     A homozygous nonsense mutation in the PLEC1 gene in patients with
            epidermolysis bullosa simplex with muscular dystrophy
  JOURNAL   J Clin Invest 98 (10), 2196-2200 (1996)
   PUBMED   8941634
REFERENCE   16 (bases 1 to 14763)
  AUTHORS   Pulkkinen,L., Smith,F.J., Shimizu,H., Murata,S., Yaoita,H.,
            Hachisuka,H., Nishikawa,T., McLean,W.H. and Uitto,J.
  TITLE     Homozygous deletion mutations in the plectin gene (PLEC1) in
            patients with epidermolysis bullosa simplex associated with
            late-onset muscular dystrophy
  JOURNAL   Hum Mol Genet 5 (10), 1539-1546 (1996)
   PUBMED   8894687
REFERENCE   17 (bases 1 to 14763)
  AUTHORS   Smith,F.J., Eady,R.A., Leigh,I.M., McMillan,J.R., Rugg,E.L.,
            Kelsell,D.P., Bryant,S.P., Spurr,N.K., Geddes,J.F., Kirtschig,G.,
            Milana,G., de Bono,A.G., Owaribe,K., Wiche,G., Pulkkinen,L.,
            Uitto,J., McLean,W.H. and Lane,E.B.
  TITLE     Plectin deficiency results in muscular dystrophy with epidermolysis
            bullosa
  JOURNAL   Nat Genet 13 (4), 450-457 (1996)
   PUBMED   8696340
REFERENCE   18 (bases 1 to 14763)
  AUTHORS   McLean,W.H., Pulkkinen,L., Smith,F.J., Rugg,E.L., Lane,E.B.,
            Bullrich,F., Burgeson,R.E., Amano,S., Hudson,D.L., Owaribe,K.,
            McGrath,J.A., McMillan,J.R., Eady,R.A., Leigh,I.M., Christiano,A.M.
            and Uitto,J.
  TITLE     Loss of plectin causes epidermolysis bullosa with muscular
            dystrophy: cDNA cloning and genomic organization
  JOURNAL   Genes Dev 10 (14), 1724-1735 (1996)
   PUBMED   8698233
REFERENCE   19 (bases 1 to 14763)
  AUTHORS   Gache,Y., Chavanas,S., Lacour,J.P., Wiche,G., Owaribe,K.,
            Meneguzzi,G. and Ortonne,J.P.
  TITLE     Defective expression of plectin/HD1 in epidermolysis bullosa
            simplex with muscular dystrophy
  JOURNAL   J Clin Invest 97 (10), 2289-2298 (1996)
   PUBMED   8636409
REFERENCE   20 (bases 1 to 14763)
  AUTHORS   Liu,C.G., Maercker,C., Castanon,M.J., Hauptmann,R. and Wiche,G.
  TITLE     Human plectin: organization of the gene, sequence analysis, and
            chromosome localization (8q24)
  JOURNAL   Proc Natl Acad Sci U S A 93 (9), 4278-4283 (1996)
   PUBMED   8633055
REFERENCE   21 (bases 1 to 14763)
  AUTHORS   Pegoraro,E. and Hoffman,E.P.
  TITLE     Limb-Girdle Muscular Dystrophy Overview - RETIRED CHAPTER, FOR
            HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301582
REFERENCE   22 (bases 1 to 14763)
  AUTHORS   So,J.Y. and Teng,J.
  TITLE     Epidermolysis Bullosa Simplex
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301543
REFERENCE   23 (bases 1 to 14763)
  AUTHORS   Abicht,A., Muller,J.S. and Lochmuller,H.
  TITLE     Congenital Myasthenic Syndromes Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301347
REFERENCE   24 (bases 1 to 14763)
  AUTHORS   Lucky,A.W. and Gorell,E.
  TITLE     Epidermolysis Bullosa with Pyloric Atresia
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301336
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff in
            collaboration with Gunther Rezniczek, Gerhard Wiche. The reference
            sequence was derived from AC109322.16, BP231761.1 and AY480049.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_201382.3.
            
            Summary: Plectin is a prominent member of an important family of
            structurally and in part functionally related proteins, termed
            plakins or cytolinkers, that are capable of interlinking different
            elements of the cytoskeleton. Plakins, with their multi-domain
            structure and enormous size, not only play crucial roles in
            maintaining cell and tissue integrity and orchestrating dynamic
            changes in cytoarchitecture and cell shape, but also serve as
            scaffolding platforms for the assembly, positioning, and regulation
            of signaling complexes (reviewed in PMID: 9701547, 11854008, and
            17499243). Plectin is expressed as several protein isoforms in a
            wide range of cell types and tissues from a single gene located on
            chromosome 8 in humans (PMID: 8633055, 8698233). Until 2010, this
            locus was named plectin 1 (symbol PLEC1 in human; Plec1 in mouse
            and rat) and the gene product had been referred to as
            'hemidesmosomal protein 1' or 'plectin 1, intermediate filament
            binding 500kDa'. These names were superseded by plectin. The
            plectin gene locus in mouse on chromosome 15 has been analyzed in
            detail (PMID: 10556294, 14559777), revealing a genomic exon-intron
            organization with well over 40 exons spanning over 62 kb and an
            unusual 5' transcript complexity of plectin isoforms. Eleven exons
            (1-1j) have been identified that alternatively splice directly into
            a common exon 2 which is the first exon to encode plectin's highly
            conserved actin binding domain (ABD). Three additional exons (-1,
            0a, and 0) splice into an alternative first coding exon (1c), and
            two additional exons (2alpha and 3alpha) are optionally spliced
            within the exons encoding the acting binding domain (exons 2-8).
            Analysis of the human locus has identified eight of the eleven
            alternative 5' exons found in mouse and rat (PMID: 14672974); exons
            1i, 1j and 1h have not been confirmed in human. Furthermore,
            isoforms lacking the central rod domain encoded by exon 31 have
            been detected in mouse (PMID:10556294), rat (PMID: 9177781), and
            human (PMID: 11441066, 10780662, 20052759). The short alternative
            amino-terminal sequences encoded by the different first exons
            direct the targeting of the various isoforms to distinct
            subcellular locations (PMID: 14559777). As the expression of
            specific plectin isoforms was found to be dependent on cell type
            (tissue) and stage of development (PMID: 10556294, 12542521,
            17389230) it appears that each cell type (tissue) contains a unique
            set (proportion and composition) of plectin isoforms, as if
            custom-made for specific requirements of the particular cells.
            Concordantly, individual isoforms were found to carry out distinct
            and specific functions (PMID: 14559777, 12542521, 18541706). In
            1996, a number of groups reported that patients suffering from
            epidermolysis bullosa simplex with muscular dystrophy (EBS-MD)
            lacked plectin expression in skin and muscle tissues due to defects
            in the plectin gene (PMID: 8698233, 8941634, 8636409, 8894687,
            8696340). Two other subtypes of plectin-related EBS have been
            described: EBS-pyloric atresia (PA) and EBS-Ogna. For reviews of
            plectin-related diseases see PMID: 15810881, 19945614. Mutations in
            the plectin gene related to human diseases should be named based on
            the position in NM_000445 (variant 1, isoform 1c), unless the
            mutation is located within one of the other alternative first
            exons, in which case the position in the respective Reference
            Sequence should be used. [provided by RefSeq, Aug 2011].
            
            Transcript Variant: This variant (8) differs in the 5' UTR and the
            5' coding region compared to variant 1. The resulting protein
            (isoform 1b) contains a distinct N-terminus compared to isoform 1c.
            Plectin isoform 1b has been found to associate exclusively with
            mitochondria (PMID: 18541706, 14559777).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY480049.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: PMID: 18541706, 17389230
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-43                AC109322.16        127910-127952
            44-88               BP231761.1         17-61
            89-14763            AY480049.1         1-14675
FEATURES             Location/Qualifiers
     source          1..14763
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.3"
     gene            1..14763
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /note="plectin"
                     /db_xref="GeneID:5339"
                     /db_xref="HGNC:HGNC:9069"
                     /db_xref="MIM:601282"
     exon            1..207
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     CDS             96..13739
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /note="isoform 1b is encoded by transcript variant 8;
                     plectin 1, intermediate filament binding protein 500kDa;
                     hemidesmosomal protein 1"
                     /codon_start=1
                     /product="plectin isoform 1b"
                     /protein_id="NP_958784.1"
                     /db_xref="CCDS:CCDS43773.1"
                     /db_xref="GeneID:5339"
                     /db_xref="HGNC:HGNC:9069"
                     /db_xref="MIM:601282"
                     /translation="MEPSGSLFPSLVVVGHVVTLAAVWHWRRGRRWAQDEQDERDRVQ
                     KKTFTKWVNKHLIKAQRHISDLYEDLRDGHNLISLLEVLSGDSLPREKGRMRFHKLQN
                     VQIALDYLRHRQVKLVNIRNDDIADGNPKLTLGLIWTIILHFQISDIQVSGQSEDMTA
                     KEKLLLWSQRMVEGYQGLRCDNFTSSWRDGRLFNAIIHRHKPLLIDMNKVYRQTNLEN
                     LDQAFSVAERDLGVTRLLDPEDVDVPQPDEKSIITYVSSLYDAMPRVPDVQDGVRANE
                     LQLRWQEYRELVLLLLQWMRHHTAAFEERRFPSSFEEIEILWSQFLKFKEMELPAKEA
                     DKNRSKGIYQSLEGAVQAGQLKVPPGYHPLDVEKEWGKLHVAILEREKQLRSEFERLE
                     CLQRIVTKLQMEAGLCEEQLNQADALLQSDVRLLAAGKVPQRAGEVERDLDKADSMIR
                     LLFNDVQTLKDGRHPQGEQMYRRVYRLHERLVAIRTEYNLRLKAGVAAPATQVAQVTL
                     QSVQRRPELEDSTLRYLQDLLAWVEENQHRVDGAEWGVDLPSVEAQLGSHRGLHQSIE
                     EFRAKIERARSDEGQLSPATRGAYRDCLGRLDLQYAKLLNSSKARLRSLESLHSFVAA
                     ATKELMWLNEKEEEEVGFDWSDRNTNMTAKKESYSALMRELELKEKKIKELQNAGDRL
                     LREDHPARPTVESFQAALQTQWSWMLQLCCCIEAHLKENAAYFQFFSDVREAEGQLQK
                     LQEALRRKYSCDRSATVTRLEDLLQDAQDEKEQLNEYKGHLSGLAKRAKAVVQLKPRH
                     PAHPMRGRLPLLAVCDYKQVEVTVHKGDECQLVGPAQPSHWKVLSSSGSEAAVPSVCF
                     LVPPPNQEAQEAVTRLEAQHQALVTLWHQLHVDMKSLLAWQSLRRDVQLIRSWSLATF
                     RTLKPEEQRQALHSLELHYQAFLRDSQDAGGFGPEDRLMAEREYGSCSHHYQQLLQSL
                     EQGAQEESRCQRCISELKDIRLQLEACETRTVHRLRLPLDKEPARECAQRIAEQQKAQ
                     AEVEGLGKGVARLSAEAEKVLALPEPSPAAPTLRSELELTLGKLEQVRSLSAIYLEKL
                     KTISLVIRGTQGAEEVLRAHEEQLKEAQAVPATLPELEATKASLKKLRAQAEAQQPTF
                     DALRDELRGAQEVGERLQQRHGERDVEVERWRERVAQLLERWQAVLAQTDVRQRELEQ
                     LGRQLRYYRESADPLGAWLQDARRRQEQIQAMPLADSQAVREQLRQEQALLEEIERHG
                     EKVEECQRFAKQYINAIKDYELQLVTYKAQLEPVASPAKKPKVQSGSESVIQEYVDLR
                     THYSELTTLTSQYIKFISETLRRMEEEERLAEQQRAEERERLAEVEAALEKQRQLAEA
                     HAQAKAQAEREAKELQQRMQEEVVRREEAAVDAQQQKRSIQEELQQLRQSSEAEIQAK
                     ARQAEAAERSRLRIEEEIRVVRLQLEATERQRGGAEGELQALRARAEEAEAQKRQAQE
                     EAERLRRQVQDESQRKRQAEVELASRVKAEAEAAREKQRALQALEELRLQAEEAERRL
                     RQAEVERARQVQVALETAQRSAEAELQSKRASFAEKTAQLERSLQEEHVAVAQLREEA
                     ERRAQQQAEAERAREEAERELERWQLKANEALRLRLQAEEVAQQKSLAQAEAEKQKEE
                     AEREARRRGKAEEQAVRQRELAEQELEKQRQLAEGTAQQRLAAEQELIRLRAETEQGE
                     QQRQLLEEELARLQREAAAATQKRQELEAELAKVRAEMEVLLASKARAEEESRSTSEK
                     SKQRLEAEAGRFRELAEEAARLRALAEEAKRQRQLAEEDAARQRAEAERVLAEKLAAI
                     GEATRLKTEAEIALKEKEAENERLRRLAEDEAFQRRRLEEQAAQHKADIEERLAQLRK
                     ASDSELERQKGLVEDTLRQRRQVEEEILALKASFEKAAAGKAELELELGRIRSNAEDT
                     LRSKEQAELEAARQRQLAAEEERRRREAEERVQKSLAAEEEAARQRKAALEEVERLKA
                     KVEEARRLRERAEQESARQLQLAQEAAQKRLQAEEKAHAFAVQQKEQELQQTLQQEQS
                     VLDQLRGEAEAARRAAEEAEEARVQAEREAAQSRRQVEEAERLKQSAEEQAQARAQAQ
                     AAAEKLRKEAEQEAARRAQAEQAALRQKQAADAEMEKHKKFAEQTLRQKAQVEQELTT
                     LRLQLEETDHQKNLLDEELQRLKAEATEAARQRSQVEEELFSVRVQMEELSKLKARIE
                     AENRALILRDKDNTQRFLQEEAEKMKQVAEEAARLSVAAQEAARLRQLAEEDLAQQRA
                     LAEKMLKEKMQAVQEATRLKAEAELLQQQKELAQEQARRLQEDKEQMAQQLAEETQGF
                     QRTLEAERQRQLEMSAEAERLKLRVAEMSRAQARAEEDAQRFRKQAEEIGEKLHRTEL
                     ATQEKVTLVQTLEIQRQQSDHDAERLREAIAELEREKEKLQQEAKLLQLKSEEMQTVQ
                     QEQLLQETQALQQSFLSEKDSLLQRERFIEQEKAKLEQLFQDEVAKAQQLREEQQRQQ
                     QQMEQERQRLVASMEEARRRQHEAEEGVRRKQEELQQLEQQRRQQEELLAEENQRLRE
                     QLQLLEEQHRAALAHSEEVTASQVAATKTLPNGRDALDGPAAEAEPEHSFDGLRRKVS
                     AQRLQEAGILSAEELQRLAQGHTTVDELARREDVRHYLQGRSSIAGLLLKATNEKLSV
                     YAALQRQLLSPGTALILLEAQAASGFLLDPVRNRRLTVNEAVKEGVVGPELHHKLLSA
                     ERAVTGYKDPYTGQQISLFQAMQKGLIVREHGIRLLEAQIATGGVIDPVHSHRVPVDV
                     AYRRGYFDEEMNRVLADPSDDTKGFFDPNTHENLTYLQLLERCVEDPETGLCLLPLTD
                     KAAKGGELVYTDSEARDVFEKATVSAPFGKFQGKTVTIWEIINSEYFTAEQRRDLLRQ
                     FRTGRITVEKIIKIIITVVEEQEQKGRLCFEGLRSLVPAAELLESRVIDRELYQQLQR
                     GERSVRDVAEVDTVRRALRGANVIAGVWLEEAGQKLSIYNALKKDLLPSDMAVALLEA
                     QAGTGHIIDPATSARLTVDEAVRAGLVGPEFHEKLLSAEKAVTGYRDPYTGQSVSLFQ
                     ALKKGLIPREQGLRLLDAQLSTGGIVDPSKSHRVPLDVACARGCLDEETSRALSAPRA
                     DAKAYSDPSTGEPATYGELQQRCRPDQLTGLSLLPLSEKAARARQEELYSELQARETF
                     EKTPVEVPVGGFKGRTVTVWELISSEYFTAEQRQELLRQFRTGKVTVEKVIKILITIV
                     EEVETLRQERLSFSGLRAPVPASELLASGVLSRAQFEQLKDGKTTVKDLSELGSVRTL
                     LQGSGCLAGIYLEDTKEKVSIYEAMRRGLLRATTAALLLEAQAATGFLVDPVRNQRLY
                     VHEAVKAGVVGPELHEQLLSAEKAVTGYRDPYSGSTISLFQAMQKGLVLRQHGIRLLE
                     AQIATGGIIDPVHSHRVPVDVAYQRGYFSEEMNRVLADPSDDTKGFFDPNTHENLTYR
                     QLLERCVEDPETGLRLLPLKGAEKAEVVETTQVYTEEETRRAFEETQIDIPGGGSHGG
                     STMSLWEVMQSDLIPEEQRAQLMADFQAGRVTKERMIIIIIEIIEKTEIIRQQGLASY
                     DYVRRRLTAEDLFEARIISLETYNLLREGTRSLREALEAESAWCYLYGTGSVAGVYLP
                     GSRQTLSIYQALKKGLLSAEVARLLLEAQAATGFLLDPVKGERLTVDEAVRKGLVGPE
                     LHDRLLSAERAVTGYRDPYTEQTISLFQAMKKELIPTEEALRLLDAQLATGGIVDPRL
                     GFHLPLEVAYQRGYLNKDTHDQLSEPSEVRSYVDPSTDERLSYTQLLRRCRRDDGTGQ
                     LLLPLSDARKLTFRGLRKQITMEELVRSQVMDEATALQLREGLTSIEEVTKNLQKFLE
                     GTSCIAGVFVDATKERLSVYQAMKKGIIRPGTAFELLEAQAATGYVIDPIKGLKLTVE
                     EAVRMGIVGPEFKDKLLSAERAVTGYKDPYSGKLISLFQAMKKGLILKDHGIRLLEAQ
                     IATGGIIDPEESHRLPVEVAYKRGLFDEEMNEILTDPSDDTKGFFDPNTEENLTYLQL
                     MERCITDPQTGLCLLPLKEKKRERKTSSKSSVRKRRVVIVDPETGKEMSVYEAYRKGL
                     IDHQTYLELSEQECEWEEITISSSDGVVKSMIIDRRSGRQYDIDDAIAKNLIDRSALD
                     QYRAGTLSITEFADMLSGNAGGFRSRSSSVGSSSSYPISPAVSRTQLASWSDPTEETG
                     PVAGILDTETLEKVSITEAMHRNLVDNITGQRLLEAQACTGGIIDPSTGERFPVTDAV
                     NKGLVDKIMVDRINLAQKAFCGFEDPRTKTKMSAAQALKKGWLYYEAGQRFLEVQYLT
                     GGLIEPDTPGRVPLDEALQRGTVDARTAQKLRDVGAYSKYLTCPKTKLKISYKDALDR
                     SMVEEGTGLRLLEAAAQSTKGYYSPYSVSGSGSTAGSRTGSRTGSRAGSRRGSFDATG
                     SGFSMTFSSSSYSSSGYGRRYASGSSASLGGPESAVA"
     exon            208..269
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            270..359
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            360..437
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            438..530
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            531..697
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            698..813
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            814..920
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1040
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1136
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1137..1264
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1358
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1359..1513
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1514..1832
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1833..1910
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1911..2072
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2073..2177
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2178..2273
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2274..2399
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2400..2552
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2553..2707
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2708..2834
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2835..3018
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3019..3176
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3177..3355
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3356..3494
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3495..3851
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3852..3935
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3936..4040
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            4041..4139
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            4140..7520
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            7521..14763
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      14743..14748
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /note="hexamer: AATAAA"
     polyA_site      14763
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /note="major polyA site"
ORIGIN      
        1 ctcgctcgcc ctgcacgcag ggagggggct gtctagcctg gagcagccgg ttcccagcgc
       61 aggaagggca gccctggcgg ctccgcgtgg cggctatgga gccctcgggc agcctgtttc
      121 cctccctggt ggttgtgggt cacgttgtca ccctggccgc tgtgtggcac tggcgcaggg
      181 gacgtcggtg ggcgcaggac gagcaagatg agcgggatcg tgtgcagaag aaaaccttca
      241 ccaagtgggt caacaagcac ctcatcaagg cccagaggca catcagtgac ctgtatgaag
      301 acctccgcga tggccacaac ctcatctccc tgctggaggt cctctcgggg gacagcctgc
      361 cccgggagaa ggggaggatg cgtttccaca agctgcagaa tgtccagatt gccctggact
      421 acctccggca ccgccaggtg aagctggtga acatcaggaa tgatgacatc gctgacggca
      481 accccaagct gacccttggc ctcatctgga caatcattct gcacttccag atctcagata
      541 tccaggtgag tgggcagtcg gaggacatga cggccaagga gaagctgctg ctgtggtcgc
      601 agcgaatggt ggaggggtac cagggcctgc gatgcgacaa cttcacctcc agctggagag
      661 acggccgcct cttcaatgcc atcatccacc ggcacaagcc cctgctcatc gacatgaaca
      721 aggtgtaccg gcagaccaac ctggagaacc tggaccaggc cttctctgtg gcggagcggg
      781 acctgggagt gacgcggctc ctggaccctg aggacgtgga tgtccctcag cccgacgaga
      841 agtccatcat cacctacgtc tcgtcgctgt atgacgccat gccccgcgtg ccggacgtgc
      901 aggatggggt gagggccaac gagctgcagc tgcgctggca ggagtaccgg gagctggtgc
      961 tgctgctgct tcagtggatg cgacaccaca cggccgcctt tgaggaacgc aggttcccct
     1021 ccagcttcga ggagattgag atcctgtggt ctcagttcct gaagtttaag gagatggagc
     1081 taccagccaa ggaggccgac aagaacaggt ccaagggcat ctaccaatcc ctggagggag
     1141 cggtgcaagc aggccagctc aaggtgcccc ctggctacca cccgctggat gtggagaagg
     1201 agtggggcaa gctgcacgtg gccatcctgg agcgggagaa gcagctccgc agcgagtttg
     1261 agaggctgga gtgtcttcag cgcatcgtga ccaagctgca gatggaggcg gggctgtgtg
     1321 aggagcagct gaaccaggcc gacgccctgc tgcagtcgga tgtccggctg ctggctgcag
     1381 gcaaagtgcc acagcgggcg ggggaggtgg aacgggactt ggacaaggcg gatagcatga
     1441 tccggctgct cttcaacgac gtgcagaccc tcaaggatgg acggcacccg cagggcgagc
     1501 agatgtaccg cagggtgtac cgtctgcacg agcgcctggt agccatccgc accgagtaca
     1561 acctacggct gaaggcaggc gtggcggccc ctgcaaccca ggtggcccag gtgactctgc
     1621 agagtgtgca gaggcgcccc gagctggagg actccactct gcgctacctg caggacctgc
     1681 tggcctgggt ggaggagaac cagcaccgtg tggatggcgc tgagtggggt gtggacctgc
     1741 ccagcgtgga ggcgcagctg ggcagccacc gaggcctgca ccagtccatc gaagaattcc
     1801 gggccaagat cgagcgggca cggagtgacg agggccagct ctcccccgcc acccggggtg
     1861 cctaccgtga ctgcctgggt cggctggacc tgcagtacgc caagctgctg aactcctcca
     1921 aggcccgcct caggtccctg gagagcttgc acagctttgt ggcagccgcc actaaggagc
     1981 taatgtggct gaatgagaag gaggaggagg aggtgggctt cgactggagc gaccgcaaca
     2041 ccaacatgac cgccaagaag gagagctact cggcgctgat gcgggagctg gagctgaagg
     2101 agaagaagat caaggagctc caaaatgctg gggaccggct gctgcgggag gaccacccgg
     2161 cccggcccac ggtggagtcc ttccaggcgg ccctgcagac gcagtggagc tggatgctac
     2221 agctgtgctg ctgtatcgag gcacacctga aggagaacgc tgcctacttt cagttcttct
     2281 cagatgtgcg ggaggccgag gggcagttgc agaagctgca ggaggcactg cgtaggaaat
     2341 acagttgtga tcgctccgcc accgtcaccc ggctggagga cctgctgcag gatgcccagg
     2401 acgagaagga acagctgaac gagtacaagg gccacctctc aggcctggcc aagcgggcca
     2461 aggccgtcgt gcagctgaag ccccgccacc cagcccaccc catgcggggc cgcctgcccc
     2521 tgctggccgt gtgcgactat aagcaggtgg aggtgactgt gcacaagggt gacgagtgcc
     2581 agctggtggg ccctgcacag ccgtcccact ggaaggtgct cagcagctcc ggcagcgagg
     2641 ccgccgtgcc ctccgtgtgc ttcctggtgc ccccgcccaa ccaggaggcc caggaggccg
     2701 tcaccaggct ggaggcccag caccaggccc tggtcacgct gtggcaccag ttgcacgtgg
     2761 acatgaagag ccttctggcc tggcagagcc ttcgccgcga cgtgcagctc atccgctcct
     2821 ggtccctggc cacgttccgc accctgaagc cagaggagca gcgccaagcc ctgcacagcc
     2881 tggagctgca ctaccaggcc ttcctgcggg acagccagga cgcgggcggc ttcggacccg
     2941 aggaccggct gatggctgag cgcgagtacg gctcctgcag ccaccactac cagcagctgc
     3001 tgcagagcct ggaacagggt gcacaggaag agtctcgctg ccagcgctgc atctccgagc
     3061 tcaaagacat ccggctgcag ctggaggcct gtgagacgcg caccgtgcac cgcctgcggc
     3121 tgccgctgga caaagagccg gcacgggagt gtgcccagcg catcgccgag cagcagaagg
     3181 cacaggcaga ggtggagggg ctgggcaagg gggtcgcccg gctctctgcc gaggccgaga
     3241 aggtcttggc cctaccagag ccatcgcctg cggcccccac gctgcgctcg gagctggagc
     3301 tgacgctggg caagctggag caggtccgca gcctgtctgc catctacctg gagaagctca
     3361 agaccatcag cctggtgatc cgcggcacgc agggggccga ggaggtgctc agggcccacg
     3421 aggagcagct caaggaggcc caggccgtgc cggccaccct cccggagctc gaggccacca
     3481 aggcctctct gaagaagctg cgggcccagg ccgaggcaca gcagcccacg ttcgacgccc
     3541 tgcgggatga gctgcggggg gcacaggagg tgggggagcg actgcagcag cggcacgggg
     3601 agcgggacgt ggaggtggag cgctggcggg agcgggtcgc ccagttgctt gagcgctggc
     3661 aggctgtgct ggcccagacc gacgtgcggc agcgcgagct cgagcaactg ggccgccagc
     3721 tgcgttacta ccgcgagagt gcagacccct tgggcgcctg gctgcaggac gccaggcggc
     3781 ggcaggagca gatccaggcc atgccgctgg ccgacagcca ggctgtgcgg gagcagctgc
     3841 ggcaggagca ggccctgctg gaggagatcg agcgccacgg cgagaaggtc gaggagtgcc
     3901 agaggtttgc gaaacagtac atcaacgcca tcaaggacta tgaactccag ctggtgacgt
     3961 acaaggcgca gcttgagccg gtggcctccc cggccaagaa gcccaaggtc cagtcgggat
     4021 cagagagtgt catccaggag tacgtggacc tgcgtacgca ctacagcgag ctgaccacac
     4081 tgacgagcca gtacatcaag ttcatcagcg agactctgcg gcgcatggag gaggaggaga
     4141 ggctggctga gcagcagcgg gcagaggagc gcgagcggct ggccgaggtg gaggccgcgc
     4201 tggagaagca gcggcagctg gccgaggcgc acgcccaggc aaaggcacag gcggagcggg
     4261 aggcgaagga gctgcagcag cgcatgcagg aggaggtggt gcggcgggag gaggcggcgg
     4321 tggacgcgca gcagcagaag cgcagcattc aggaggagct gcagcagctg cggcagagct
     4381 cggaggcgga gatccaggcc aaggcccggc aggcagaggc ggctgagcgc agccggctgc
     4441 gcatcgagga ggagatccgc gtggtgcgcc tgcagttgga ggccaccgag cgccagcgtg
     4501 gcggggctga gggggagctg caggcactgc gtgcacgggc ggaggaggct gaggcacaaa
     4561 agcgacaggc gcaggaggag gccgagcgct tgcggaggca ggtgcaggac gagagccagc
     4621 gtaagcggca ggcggaggtg gagctggcct cgcgcgtgaa ggccgaggcc gaggcggcgc
     4681 gcgagaagca gcgggccctg caggccctgg aggagctgcg gctgcaggcg gaggaggcgg
     4741 agcggcgcct gcggcaggcc gaggtggagc gagcgcggca ggtacaggtg gccctggaga
     4801 cggcgcagcg cagtgcagag gcggagctgc agagcaaacg cgcctccttc gccgagaaga
     4861 cggcacagct ggagcgctcc ctgcaggagg aacacgtggc tgtggcacag ctgcgggagg
     4921 aggctgagcg gcgggcacag cagcaggccg aggccgagcg ggcgcgcgag gaggcagagc
     4981 gggagctgga gcgctggcag ctcaaggcca acgaggcgct acggctgcgg ctgcaggcgg
     5041 aggaggtggc gcagcagaag agcctggcgc aggccgaggc tgagaagcag aaggaggagg
     5101 cggagcgcga ggcgcggcgg cgcggcaagg cggaggagca ggccgtccgg cagcgggagc
     5161 tggctgaaca agagctggag aagcagcggc agctggcgga aggcaccgcg cagcagcgcc
     5221 tggccgcgga gcaggagttg atccggctgc gggccgagac ggagcagggg gagcagcagc
     5281 ggcagctgct ggaggaggag ctggcccggc tgcagcgtga ggcggctgca gccacgcaga
     5341 aacggcagga gctggaagcc gagctggcca aggtgcgggc cgagatggag gtgctgctgg
     5401 ccagcaaggc gagggctgag gaggagtcgc gctccaccag cgagaagtcc aagcagaggc
     5461 tggaggccga ggccggccgg ttccgcgagc tggccgagga ggccgcccgc ctgcgtgccc
     5521 tggcggaaga ggccaagcgg cagcggcagc tggccgagga agacgcggcg cggcagcggg
     5581 ccgaggcgga gcgggtgctt gcggagaagc tggccgccat cggcgaggcc acgcggctca
     5641 agacggaggc ggagatcgcg ctcaaggaga aggaggcgga gaacgagcgc ctgcggcggc
     5701 tggcggagga cgaggccttc cagcggcggc ggctggagga gcaggccgcg caacacaagg
     5761 ctgacatcga ggagcgcctg gcccagctgc gcaaggcatc ggacagcgag ctggagcggc
     5821 agaaggggct ggtggaggac acgctgaggc agcggcggca ggtggaggag gagatcctgg
     5881 cgctgaaggc gagcttcgag aaggcggccg ctggcaaggc ggagctggag ctggagctgg
     5941 gacgcatccg cagcaacgcg gaggacacgc tgcgcagcaa ggagcaggcc gagctggagg
     6001 ctgcgaggca gcggcagctg gcggcggagg aggagcggcg gcgccgtgag gctgaggagc
     6061 gcgtgcagaa gagcctggcg gccgaggagg aggccgcacg gcagcggaag gcggcgctgg
     6121 aggaagtcga gcggctgaaa gccaaggtgg aggaggcgcg gcgcctgcgg gagcgagcgg
     6181 agcaggagtc ggcgcggcag ctgcagctgg cccaggaggc cgcccagaag cggctgcagg
     6241 cggaagagaa ggcacacgcc ttcgcggtgc agcagaagga gcaggagcta cagcagacgc
     6301 tgcagcagga gcagagcgtg ctggaccagc tgcgcggcga ggcggaggcg gcccggcggg
     6361 cggctgagga ggcggaggag gcccgggtgc aggcggagcg tgaggcggcg cagtcccggc
     6421 ggcaggtgga agaggccgag cggctgaagc agtcggcaga ggagcaggca caggcccggg
     6481 ctcaggcaca ggcggctgca gagaagctgc gcaaggaggc cgagcaagag gcggcgcggc
     6541 gggcacaggc ggagcaggcg gccctgcggc agaagcaggc agctgacgcg gagatggaga
     6601 agcataagaa attcgccgag cagacgctgc ggcagaaggc gcaggtggag caggagctga
     6661 caacactgcg gctgcagctg gaggagaccg accaccagaa gaacctgctg gacgaggagc
     6721 tgcagcggct gaaggcggag gccacggagg ccgcacgcca gcgcagccag gtggaggagg
     6781 agctcttctc ggtgcgcgtg cagatggagg agctgagcaa gctcaaggca cgcatcgagg
     6841 ctgagaaccg cgcactcatc ttgcgtgaca aggacaatac gcagcgcttc ctgcaggagg
     6901 aggctgagaa gatgaagcag gtggcggagg aggccgcgcg gctgagtgtg gcggcccaag
     6961 aggctgcgcg actgcggcag ctggcagagg aggacctggc acagcagcgg gccttggcag
     7021 agaagatgct caaggagaag atgcaggcgg tgcaggaggc cacgcgactc aaggctgagg
     7081 cggaactgct gcagcagcag aaggagcttg cgcaggagca ggcgcggcgg ctgcaggagg
     7141 acaaggagca gatggcgcag cagctggcgg aggagacgca gggcttccag cggacgctgg
     7201 aggccgagcg gcagcggcag ctggagatga gcgctgaggc tgagcgcctc aagctgcgtg
     7261 tggccgagat gagccgagcc caggcccgcg ctgaggagga cgcccagcgc ttccggaagc
     7321 aggcggagga gatcggtgag aagctgcacc gcacggagct cgccacccag gagaaggtga
     7381 ccctggtgca gacactggag atccagcgac agcagagtga ccatgatgcc gagcgcctgc
     7441 gggaggccat cgctgagctg gagcgtgaga aggagaagct ccaacaggag gccaaactgc
     7501 tgcagctcaa gtctgaggag atgcagacgg tgcagcagga gcagctgctg caggagacgc
     7561 aggccctgca gcaaagcttc ctctctgaaa aggacagcct gctacagcgg gagcgcttca
     7621 tcgagcagga gaaggccaag ctggagcagc tcttccagga cgaggtggcc aaggcacagc
     7681 agctgcgtga ggagcagcag cggcagcagc agcagatgga gcaggaacgg cagcggctgg
     7741 tggccagcat ggaggaggcg cggcggcggc agcatgaggc cgaggagggc gtgcggcgca
     7801 agcaggagga gctgcagcag ctggagcagc agcggcggca gcaggaggag ctgctggctg
     7861 aggagaacca gaggctgcgt gagcagctgc agctcctgga ggagcagcac cgggccgcgc
     7921 tggcgcactc agaggaggtc actgcctcgc aggtggctgc cacaaagacc ctgcccaatg
     7981 gccgggatgc acttgatggc cccgcggcag aggcagagcc ggagcacagc ttcgatggcc
     8041 tgcggcggaa ggtgtcagct cagaggctgc aggaggccgg catcctgagt gcggaggagc
     8101 tgcagcggtt ggcgcagggc cacaccacgg tggacgagct cgcacggcgg gaagacgtgc
     8161 gccactacct gcagggccgc agcagtatcg cagggctgtt gctgaaggcc accaatgaga
     8221 agctgagtgt ttacgccgcc ctgcagaggc agctgctgag tcccggcacg gccctcatcc
     8281 tgctggaggc gcaggcggcc tcaggcttcc tgctggaccc tgtgcggaac cggcggctga
     8341 ccgtcaacga ggctgtgaag gagggtgtgg tgggccccga gctgcaccac aagctgctgt
     8401 cggccgagcg cgccgtcact ggctacaagg acccctacac tggccagcag atctctctct
     8461 tccaagccat gcagaagggc ctcatcgtcc gggagcacgg catccgcctg ctggaggccc
     8521 agatcgccac gggcggcgtt atcgaccccg tgcacagcca ccgcgtgccc gtggacgtgg
     8581 cctaccggcg cggctacttc gacgaggaga tgaaccgcgt cctggcggac cccagcgacg
     8641 acaccaaggg cttctttgac cccaacacgc acgagaacct cacgtacctg cagctactgg
     8701 agcgctgcgt ggaggacccc gagacgggcc tgtgccttct gccactcacg gataaggctg
     8761 ccaagggcgg ggagctggtc tacactgact ccgaggcccg ggacgtcttt gagaaggcca
     8821 ccgtgtctgc gccgttcggc aagttccagg gcaagacggt gaccatttgg gagatcatca
     8881 actcggaata cttcacggca gagcagcggc gggacctgct gcggcagttc cgcacgggcc
     8941 ggatcacagt ggagaagatc atcaagatca tcatcacggt ggtggaggag caggagcaga
     9001 agggccggct ttgctttgag ggcctgcgca gcctggtgcc agccgccgag ctgctggaga
     9061 gcagggtcat cgaccgcgag ctctaccagc agctgcagcg aggtgagcgc tctgtgcgag
     9121 acgtagccga ggtggacact gtgcggcggg ctctccgggg tgccaacgtc atcgcgggtg
     9181 tatggctgga ggaggcgggg cagaagctga gtatctacaa tgccctgaag aaagacctgc
     9241 tgccatccga catggccgtg gccctgttgg aagcccaggc cggcaccggg cacatcatcg
     9301 accccgccac cagcgcccgg ctgaccgtgg acgaggcagt gcgtgctggc ctggtgggcc
     9361 ccgagtttca tgagaagctg ctatcagccg agaaggctgt gacagggtac agggacccct
     9421 acacagggca gagcgtctcc ctgttccagg ccctgaagaa gggcctcatt ccccgggagc
     9481 agggcctgcg cctgttggac gcccagctgt ccacgggcgg catcgtggac cccagcaaga
     9541 gccaccgcgt gcccctggat gtcgcctgcg cccgaggctg cctggatgag gagaccagca
     9601 gggccctgtc ggcaccaagg gccgacgcca aggcctacag tgaccccagc acaggggagc
     9661 cggccaccta cggcgagctc cagcagcggt gccggcccga ccagctgacc gggctgagcc
     9721 tgctgccgct ctcagaaaag gctgctcggg cccggcagga ggagctctac tcagagctgc
     9781 aggcccgtga gacctttgaa aagaccccgg ttgaggtccc cgtgggtggc ttcaagggca
     9841 ggacggtgac ggtgtgggag ctcatcagct ctgagtactt cactgcggag cagcggcagg
     9901 agctgttgcg tcagttccgc acgggcaagg tcaccgtgga gaaggtcatc aagattctca
     9961 ttaccatcgt ggaggaggtg gagaccctgc ggcaggagag gctgtccttc agcggcctcc
    10021 gtgcccctgt gccagccagc gagctcctgg cttccggggt cctcagcaga gcccagtttg
    10081 agcagctcaa ggacggcaag acgacggtca aggacctttc ggagctgggc tccgtgcgga
    10141 cgctgctgca gggcagtggc tgcctcgccg gcatctacct ggaggacacc aaggagaagg
    10201 tgtccatcta cgaggccatg cgccggggcc tgctgagagc cacaacggct gcgctcctgc
    10261 tggaggcgca ggcggccact ggcttcctgg tggaccccgt gcggaaccag cgcctgtatg
    10321 tccacgaggc cgtgaaggcg ggcgtggtgg gccccgagct tcacgagcag ctgctgtctg
    10381 ccgagaaggc cgtcaccggc tacagagacc cctactcggg cagcaccatc tccctcttcc
    10441 aggccatgca gaagggcctg gttctccggc agcacggcat ccgcctgctg gaggcccaga
    10501 tcgccacggg cggcatcatc gaccccgtgc acagccaccg cgtgcctgtg gacgtggcct
    10561 accagcgcgg ctacttcagt gaggagatga accgcgtcct ggcggacccc agcgacgaca
    10621 ccaagggctt ctttgacccc aacacgcatg agaacctcac gtacaggcag ctgctggagc
    10681 ggtgcgtgga ggaccccgag acgggcttgc gccttctgcc actgaaaggg gcggagaagg
    10741 ctgaggtggt ggagaccacg caggtgtaca ctgaggagga gacaagaagg gcatttgaag
    10801 agacacagat cgacattccc ggcggcggca gccacggcgg ctccaccatg tccctgtggg
    10861 aggtgatgca gtcggacctg atccccgagg agcagcgggc ccagctgatg gctgacttcc
    10921 aggccggccg ggtgaccaag gaacgcatga tcatcatcat catcgagatc attgagaaga
    10981 cagagatcat ccgccagcag ggtctggcct cctacgacta cgtgcgccgc cgcctcacgg
    11041 ctgaggacct gttcgaggct cggatcatct ctctcgagac ctacaacctg ctccgggagg
    11101 gcaccaggag cctccgtgag gctctcgagg cggagtccgc ctggtgctac ctctatggca
    11161 cgggctccgt ggctggtgtc tacctgcccg gttccaggca gacactgagc atctaccagg
    11221 ctctcaagaa agggctgctg agtgccgagg tggcccgcct gctgctggag gcacaggcag
    11281 ccacaggctt cctgctggac ccggtgaagg gggagcggct gactgtggat gaggctgtgc
    11341 ggaagggcct cgtggggccc gagctgcacg accgcctgct ctcggctgag cgggcggtca
    11401 ccggctaccg tgacccctac accgagcaga ccatctcgct cttccaggcc atgaagaagg
    11461 agctgatccc tactgaggag gccctgcggc tgctggatgc ccagctggcc accggcggca
    11521 tcgtggaccc ccgcctgggc ttccaccttc ccctggaggt ggcttaccag cgtggctacc
    11581 tcaacaagga cacgcacgac cagctgtcag agcccagcga ggtgcgcagc tacgtggacc
    11641 cgtccaccga cgagcgcctc agctacacgc agctgctcag gcggtgccgt cgtgacgacg
    11701 gcaccggcca gctgctcctg ccactgtcgg acgcccgcaa gctgaccttc cgtggcctgc
    11761 ggaagcagat caccatggag gagctggtgc gctcgcaggt catggacgag gccacggcgc
    11821 tgcagctgcg ggagggcctg acctccatcg aggaggtcac caagaacttg cagaagttcc
    11881 tggaaggcac cagctgcatc gctggtgtct tcgtggacgc caccaaggaa cggctctcgg
    11941 tgtaccaggc catgaagaag ggcatcatcc gccccggcac agcctttgag ctcctggagg
    12001 cgcaggcggc caccggttac gtcatcgacc ccatcaaggg actgaagctg acggtggagg
    12061 aggctgtgcg tatgggcatt gtgggccccg agttcaagga caagctgctg tcggccgagc
    12121 gcgccgtcac tgggtacaag gacccctact ctgggaagct catctccctc ttccaggcca
    12181 tgaagaaggg cctgatcctg aaggaccatg gcatccgcct gctggaggcc cagatcgcca
    12241 cgggcggcat catcgaccct gaggagagcc accggctgcc cgtggaggtg gcctacaagc
    12301 gcggcctctt cgatgaggag atgaacgaga tcctgaccga cccctcggac gacaccaagg
    12361 gcttctttga ccctaacacg gaggagaacc tcacctacct gcagctgatg gagcgttgta
    12421 tcactgaccc ccagacgggc ctgtgtctct tgccgctgaa ggagaagaag cgggagcgga
    12481 agacgtcctc caagtcctcc gtgcgcaagc gccgagtggt catcgtggac cccgagacgg
    12541 gcaaggagat gtcagtgtac gaggcctacc gcaagggcct gattgaccac cagacgtacc
    12601 tggagctgtc cgagcaggag tgcgagtggg aggagatcac catctcctcc tcggacggcg
    12661 tggtcaagtc catgatcatc gaccgccgct ccgggcgcca gtacgacatc gatgatgcca
    12721 tcgccaagaa cctcatcgac cgctcggcac tggaccagta ccgcgccggc acgctctcca
    12781 tcaccgagtt cgccgacatg ctctcgggca acgccggtgg tttccgctcc cgttcctcct
    12841 cggtgggatc ctcctcctcc taccccatca gccccgccgt ctccaggacc cagctggcct
    12901 cctggtcaga ccccactgag gagacgggcc ccgtggctgg catcctggac acggagacgc
    12961 tggagaaggt gtccatcacc gaggccatgc accggaacct ggtggataac atcacggggc
    13021 agcggctgct ggaggcgcag gcctgcaccg ggggcatcat cgaccccagc accggtgagc
    13081 gcttccctgt caccgacgcc gtcaacaagg gcctggtgga caagatcatg gtggaccgca
    13141 tcaacctggc ccagaaggcc ttctgcggct tcgaggaccc acgcaccaag accaagatgt
    13201 cggccgccca ggccctgaag aagggctggc tctactacga ggccggccag cgcttcctgg
    13261 aggtgcagta cctgaccggc ggcttgatcg agcccgacac gccgggccgc gtgcccctgg
    13321 acgaggccct gcagcgcggc acggtggacg cccgcaccgc acagaagctg cgtgacgtgg
    13381 gcgcctactc caagtacctc acctgcccta agaccaagct caagatctcc tataaggacg
    13441 cgctggaccg cagcatggtg gaggagggca cggggctgcg gctgctggag gctgccgcgc
    13501 agtccaccaa gggctactac agcccctaca gcgtcagcgg ctccggctct accgctggct
    13561 cccgcaccgg ctcgcgcacc ggctcccggg ccggctcccg ccgcggcagc tttgacgcca
    13621 ccggctccgg cttctccatg accttctctt catcctccta ctcctcctcg ggctacggcc
    13681 gccgctacgc ctcggggtcc tcggcctccc tggggggccc tgagtctgcc gtggcctgag
    13741 gctgcctgcg cccaccccgc tctgcatgcg gcccagcccg gctcccaccg aggcgcgggg
    13801 gccgttttca acgcttaaag gtgtcttcct cccaagtggt gcctaaagtt taaccaaaaa
    13861 gaccagacta atatattaat atatatctgc tgtccagaca gcctgtatct tgggggacag
    13921 ggctggccca gccctgctgg ccgcctcacc ccctcgggtc tcctcactcc cttctacctg
    13981 ccactcacac agccaggtgc cttggagggt cccaagctgg gccccagccc accctcctgt
    14041 cttcccaggg tagcccgcct gccagtccta gctgcacagg gcagctgggc ccaaccctgt
    14101 ctgtagaggg ccctggtgtt tctagcactg gcctgcacgg tgggccttgc tggggacggg
    14161 gggccccagt cagcctctct cccagtctac ccagagaagc cccttcccca tgggaagacg
    14221 aggccctcgg gcccagcccc cacagtgctg tctgatctgt gctttccagc tcacccccca
    14281 cactcactcc tgagacccct ggcctccggc gtcagcctcc agcctctgtt cccctagtaa
    14341 gtgccttcca tgtcggcctc taaccccagg ccccgaggac ccagacccag tggggaggcg
    14401 gacgttccag ccggcatggc tgggaactgc agacctgtcc tcctggtggg tccaggggcc
    14461 cctccagctt gtggagcccc acactggggt gccgcctgcc cgtctctctc ccatggagcc
    14521 ccagccccct ttgggcccag ggacaccagc caggctctgt gctgaccctc ctgttgcacc
    14581 cagccctggt ctcagcagcg accacccctg cctccaccct ctgagctttg catgttccac
    14641 taaccccggg cgggtggcag gtggaggtgt caggctgctg gcgcctctgc aagggcagaa
    14701 cactaacctg accgtgggcg gggccttgcg gtatccgccc ccaataaaag caattccaac
    14761 ctt
//
