LOCUS       NM_201381              14679 bp    mRNA    linear   PRI 11-OCT-2024
DEFINITION  Homo sapiens plectin (PLEC), transcript variant 7, mRNA.
ACCESSION   NM_201381
VERSION     NM_201381.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 14679)
  AUTHORS   Rezniczek,G.A., Walko,G. and Wiche,G.
  TITLE     Plectin gene defects lead to various forms of epidermolysis bullosa
            simplex
  JOURNAL   Dermatol Clin 28 (1), 33-41 (2010)
   PUBMED   19945614
  REMARK    Review article
            Erratum:[Dermatol Clin. 2010 Apr;28(2):439-41]
REFERENCE   2  (bases 1 to 14679)
  AUTHORS   Winter,L., Abrahamsberg,C. and Wiche,G.
  TITLE     Plectin isoform 1b mediates mitochondrion-intermediate filament
            network linkage and controls organelle shape
  JOURNAL   J Cell Biol 181 (6), 903-911 (2008)
   PUBMED   18541706
REFERENCE   3  (bases 1 to 14679)
  AUTHORS   Sonnenberg,A. and Liem,R.K.
  TITLE     Plakins in development and disease
  JOURNAL   Exp Cell Res 313 (10), 2189-2203 (2007)
   PUBMED   17499243
  REMARK    Review article
REFERENCE   4  (bases 1 to 14679)
  AUTHORS   Rezniczek,G.A., Konieczny,P., Nikolic,B., Reipert,S., Schneller,D.,
            Abrahamsberg,C., Davies,K.E., Winder,S.J. and Wiche,G.
  TITLE     Plectin 1f scaffolding at the sarcolemma of dystrophic (mdx) muscle
            fibers through multiple interactions with beta-dystroglycan
  JOURNAL   J Cell Biol 176 (7), 965-977 (2007)
   PUBMED   17389230
REFERENCE   5  (bases 1 to 14679)
  AUTHORS   Pfendner,E., Rouan,F. and Uitto,J.
  TITLE     Progress in epidermolysis bullosa: the phenotypic spectrum of
            plectin mutations
  JOURNAL   Exp Dermatol 14 (4), 241-249 (2005)
   PUBMED   15810881
  REMARK    GeneRIF: discussion of phenotypic spectrum of plectin mutations
            [review]
            Review article
REFERENCE   6  (bases 1 to 14679)
  AUTHORS   Zhang,T., Haws,P. and Wu,Q.
  TITLE     Multiple variable first exons: a mechanism for cell- and
            tissue-specific gene regulation
  JOURNAL   Genome Res 14 (1), 79-89 (2004)
   PUBMED   14672974
REFERENCE   7  (bases 1 to 14679)
  AUTHORS   Rezniczek,G.A., Abrahamsberg,C., Fuchs,P., Spazierer,D. and
            Wiche,G.
  TITLE     Plectin 5'-transcript diversity: short alternative sequences
            determine stability of gene products, initiation of translation and
            subcellular localization of isoforms
  JOURNAL   Hum Mol Genet 12 (23), 3181-3194 (2003)
   PUBMED   14559777
REFERENCE   8  (bases 1 to 14679)
  AUTHORS   Andra,K., Kornacker,I., Jorgl,A., Zorer,M., Spazierer,D., Fuchs,P.,
            Fischer,I. and Wiche,G.
  TITLE     Plectin-isoform-specific rescue of hemidesmosomal defects in
            plectin (-/-) keratinocytes
  JOURNAL   J Invest Dermatol 120 (2), 189-197 (2003)
   PUBMED   12542521
REFERENCE   9  (bases 1 to 14679)
  AUTHORS   Leung,C.L., Green,K.J. and Liem,R.K.
  TITLE     Plakins: a family of versatile cytolinker proteins
  JOURNAL   Trends Cell Biol 12 (1), 37-45 (2002)
   PUBMED   11854008
  REMARK    Review article
REFERENCE   10 (bases 1 to 14679)
  AUTHORS   Brown,M.J., Hallam,J.A., Liu,Y., Yamada,K.M. and Shaw,S.
  TITLE     Cutting edge: integration of human T lymphocyte cytoskeleton by the
            cytolinker plectin
  JOURNAL   J Immunol 167 (2), 641-645 (2001)
   PUBMED   11441066
  REMARK    GeneRIF: During polarization of peripheral blood T lymphocytes,
            plectin redistributes to the uropod associated with vimentin and
            fodrin. This vimentin-plectin-fodrin complex provides a continuous
            linkage from the nucleus (lamin B) to the cortical cytoskeleton.
REFERENCE   11 (bases 1 to 14679)
  AUTHORS   Schroder,R., Furst,D.O., Klasen,C., Reimann,J., Herrmann,H. and van
            der Ven,P.F.
  TITLE     Association of plectin with Z-discs is a prerequisite for the
            formation of the intermyofibrillar desmin cytoskeleton
  JOURNAL   Lab Invest 80 (4), 455-464 (2000)
   PUBMED   10780662
REFERENCE   12 (bases 1 to 14679)
  AUTHORS   Fuchs,P., Zorer,M., Rezniczek,G.A., Spazierer,D., Oehler,S.,
            Castanon,M.J., Hauptmann,R. and Wiche,G.
  TITLE     Unusual 5' transcript complexity of plectin isoforms: novel
            tissue-specific exons modulate actin binding activity
  JOURNAL   Hum Mol Genet 8 (13), 2461-2472 (1999)
   PUBMED   10556294
REFERENCE   13 (bases 1 to 14679)
  AUTHORS   Wiche,G.
  TITLE     Role of plectin in cytoskeleton organization and dynamics
  JOURNAL   J Cell Sci 111 (Pt 17), 2477-2486 (1998)
   PUBMED   9701547
  REMARK    Review article
REFERENCE   14 (bases 1 to 14679)
  AUTHORS   Elliott,C.E., Becker,B., Oehler,S., Castanon,M.J., Hauptmann,R. and
            Wiche,G.
  TITLE     Plectin transcript diversity: identification and tissue
            distribution of variants with distinct first coding exons and
            rodless isoforms
  JOURNAL   Genomics 42 (1), 115-125 (1997)
   PUBMED   9177781
REFERENCE   15 (bases 1 to 14679)
  AUTHORS   Chavanas,S., Pulkkinen,L., Gache,Y., Smith,F.J., McLean,W.H.,
            Uitto,J., Ortonne,J.P. and Meneguzzi,G.
  TITLE     A homozygous nonsense mutation in the PLEC1 gene in patients with
            epidermolysis bullosa simplex with muscular dystrophy
  JOURNAL   J Clin Invest 98 (10), 2196-2200 (1996)
   PUBMED   8941634
REFERENCE   16 (bases 1 to 14679)
  AUTHORS   Pulkkinen,L., Smith,F.J., Shimizu,H., Murata,S., Yaoita,H.,
            Hachisuka,H., Nishikawa,T., McLean,W.H. and Uitto,J.
  TITLE     Homozygous deletion mutations in the plectin gene (PLEC1) in
            patients with epidermolysis bullosa simplex associated with
            late-onset muscular dystrophy
  JOURNAL   Hum Mol Genet 5 (10), 1539-1546 (1996)
   PUBMED   8894687
REFERENCE   17 (bases 1 to 14679)
  AUTHORS   Smith,F.J., Eady,R.A., Leigh,I.M., McMillan,J.R., Rugg,E.L.,
            Kelsell,D.P., Bryant,S.P., Spurr,N.K., Geddes,J.F., Kirtschig,G.,
            Milana,G., de Bono,A.G., Owaribe,K., Wiche,G., Pulkkinen,L.,
            Uitto,J., McLean,W.H. and Lane,E.B.
  TITLE     Plectin deficiency results in muscular dystrophy with epidermolysis
            bullosa
  JOURNAL   Nat Genet 13 (4), 450-457 (1996)
   PUBMED   8696340
REFERENCE   18 (bases 1 to 14679)
  AUTHORS   McLean,W.H., Pulkkinen,L., Smith,F.J., Rugg,E.L., Lane,E.B.,
            Bullrich,F., Burgeson,R.E., Amano,S., Hudson,D.L., Owaribe,K.,
            McGrath,J.A., McMillan,J.R., Eady,R.A., Leigh,I.M., Christiano,A.M.
            and Uitto,J.
  TITLE     Loss of plectin causes epidermolysis bullosa with muscular
            dystrophy: cDNA cloning and genomic organization
  JOURNAL   Genes Dev 10 (14), 1724-1735 (1996)
   PUBMED   8698233
REFERENCE   19 (bases 1 to 14679)
  AUTHORS   Gache,Y., Chavanas,S., Lacour,J.P., Wiche,G., Owaribe,K.,
            Meneguzzi,G. and Ortonne,J.P.
  TITLE     Defective expression of plectin/HD1 in epidermolysis bullosa
            simplex with muscular dystrophy
  JOURNAL   J Clin Invest 97 (10), 2289-2298 (1996)
   PUBMED   8636409
REFERENCE   20 (bases 1 to 14679)
  AUTHORS   Liu,C.G., Maercker,C., Castanon,M.J., Hauptmann,R. and Wiche,G.
  TITLE     Human plectin: organization of the gene, sequence analysis, and
            chromosome localization (8q24)
  JOURNAL   Proc Natl Acad Sci U S A 93 (9), 4278-4283 (1996)
   PUBMED   8633055
REFERENCE   21 (bases 1 to 14679)
  AUTHORS   Pegoraro,E. and Hoffman,E.P.
  TITLE     Limb-Girdle Muscular Dystrophy Overview - RETIRED CHAPTER, FOR
            HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301582
REFERENCE   22 (bases 1 to 14679)
  AUTHORS   So,J.Y. and Teng,J.
  TITLE     Epidermolysis Bullosa Simplex
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301543
REFERENCE   23 (bases 1 to 14679)
  AUTHORS   Abicht,A., Muller,J.S. and Lochmuller,H.
  TITLE     Congenital Myasthenic Syndromes Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301347
REFERENCE   24 (bases 1 to 14679)
  AUTHORS   Lucky,A.W. and Gorell,E.
  TITLE     Epidermolysis Bullosa with Pyloric Atresia
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301336
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff in
            collaboration with Gunther Rezniczek, Gerhard Wiche. The reference
            sequence was derived from AC109322.16 and AY480048.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_201381.2.
            
            Summary: Plectin is a prominent member of an important family of
            structurally and in part functionally related proteins, termed
            plakins or cytolinkers, that are capable of interlinking different
            elements of the cytoskeleton. Plakins, with their multi-domain
            structure and enormous size, not only play crucial roles in
            maintaining cell and tissue integrity and orchestrating dynamic
            changes in cytoarchitecture and cell shape, but also serve as
            scaffolding platforms for the assembly, positioning, and regulation
            of signaling complexes (reviewed in PMID: 9701547, 11854008, and
            17499243). Plectin is expressed as several protein isoforms in a
            wide range of cell types and tissues from a single gene located on
            chromosome 8 in humans (PMID: 8633055, 8698233). Until 2010, this
            locus was named plectin 1 (symbol PLEC1 in human; Plec1 in mouse
            and rat) and the gene product had been referred to as
            'hemidesmosomal protein 1' or 'plectin 1, intermediate filament
            binding 500kDa'. These names were superseded by plectin. The
            plectin gene locus in mouse on chromosome 15 has been analyzed in
            detail (PMID: 10556294, 14559777), revealing a genomic exon-intron
            organization with well over 40 exons spanning over 62 kb and an
            unusual 5' transcript complexity of plectin isoforms. Eleven exons
            (1-1j) have been identified that alternatively splice directly into
            a common exon 2 which is the first exon to encode plectin's highly
            conserved actin binding domain (ABD). Three additional exons (-1,
            0a, and 0) splice into an alternative first coding exon (1c), and
            two additional exons (2alpha and 3alpha) are optionally spliced
            within the exons encoding the acting binding domain (exons 2-8).
            Analysis of the human locus has identified eight of the eleven
            alternative 5' exons found in mouse and rat (PMID: 14672974); exons
            1i, 1j and 1h have not been confirmed in human. Furthermore,
            isoforms lacking the central rod domain encoded by exon 31 have
            been detected in mouse (PMID:10556294), rat (PMID: 9177781), and
            human (PMID: 11441066, 10780662, 20052759). The short alternative
            amino-terminal sequences encoded by the different first exons
            direct the targeting of the various isoforms to distinct
            subcellular locations (PMID: 14559777). As the expression of
            specific plectin isoforms was found to be dependent on cell type
            (tissue) and stage of development (PMID: 10556294, 12542521,
            17389230) it appears that each cell type (tissue) contains a unique
            set (proportion and composition) of plectin isoforms, as if
            custom-made for specific requirements of the particular cells.
            Concordantly, individual isoforms were found to carry out distinct
            and specific functions (PMID: 14559777, 12542521, 18541706). In
            1996, a number of groups reported that patients suffering from
            epidermolysis bullosa simplex with muscular dystrophy (EBS-MD)
            lacked plectin expression in skin and muscle tissues due to defects
            in the plectin gene (PMID: 8698233, 8941634, 8636409, 8894687,
            8696340). Two other subtypes of plectin-related EBS have been
            described: EBS-pyloric atresia (PA) and EBS-Ogna. For reviews of
            plectin-related diseases see PMID: 15810881, 19945614. Mutations in
            the plectin gene related to human diseases should be named based on
            the position in NM_000445 (variant 1, isoform 1c), unless the
            mutation is located within one of the other alternative first
            exons, in which case the position in the respective Reference
            Sequence should be used. [provided by RefSeq, Aug 2011].
            
            Transcript Variant: This variant (7) differs in the 5' UTR and the
            5' coding region compared to variant 1. The resulting protein
            (isoform 1d) contains a distinct N-terminus compared to isoform 1c.
            Plectin isoform 1d has been found associated with Z-disks in
            skeletal muscle (PMID: 17389230).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY480048.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: PMID: 18541706, 17389230
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-33                AC109322.16        127125-127157
            34-14679            AY480048.1         1-14646
FEATURES             Location/Qualifiers
     source          1..14679
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.3"
     gene            1..14679
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /note="plectin"
                     /db_xref="GeneID:5339"
                     /db_xref="HGNC:HGNC:9069"
                     /db_xref="MIM:601282"
     exon            1..123
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     CDS             108..13655
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /note="isoform 1d is encoded by transcript variant 7;
                     plectin 1, intermediate filament binding protein 500kDa;
                     hemidesmosomal protein 1"
                     /codon_start=1
                     /product="plectin isoform 1d"
                     /protein_id="NP_958783.1"
                     /db_xref="CCDS:CCDS47936.1"
                     /db_xref="GeneID:5339"
                     /db_xref="HGNC:HGNC:9069"
                     /db_xref="MIM:601282"
                     /translation="MKIVPDERDRVQKKTFTKWVNKHLIKAQRHISDLYEDLRDGHNL
                     ISLLEVLSGDSLPREKGRMRFHKLQNVQIALDYLRHRQVKLVNIRNDDIADGNPKLTL
                     GLIWTIILHFQISDIQVSGQSEDMTAKEKLLLWSQRMVEGYQGLRCDNFTSSWRDGRL
                     FNAIIHRHKPLLIDMNKVYRQTNLENLDQAFSVAERDLGVTRLLDPEDVDVPQPDEKS
                     IITYVSSLYDAMPRVPDVQDGVRANELQLRWQEYRELVLLLLQWMRHHTAAFEERRFP
                     SSFEEIEILWSQFLKFKEMELPAKEADKNRSKGIYQSLEGAVQAGQLKVPPGYHPLDV
                     EKEWGKLHVAILEREKQLRSEFERLECLQRIVTKLQMEAGLCEEQLNQADALLQSDVR
                     LLAAGKVPQRAGEVERDLDKADSMIRLLFNDVQTLKDGRHPQGEQMYRRVYRLHERLV
                     AIRTEYNLRLKAGVAAPATQVAQVTLQSVQRRPELEDSTLRYLQDLLAWVEENQHRVD
                     GAEWGVDLPSVEAQLGSHRGLHQSIEEFRAKIERARSDEGQLSPATRGAYRDCLGRLD
                     LQYAKLLNSSKARLRSLESLHSFVAAATKELMWLNEKEEEEVGFDWSDRNTNMTAKKE
                     SYSALMRELELKEKKIKELQNAGDRLLREDHPARPTVESFQAALQTQWSWMLQLCCCI
                     EAHLKENAAYFQFFSDVREAEGQLQKLQEALRRKYSCDRSATVTRLEDLLQDAQDEKE
                     QLNEYKGHLSGLAKRAKAVVQLKPRHPAHPMRGRLPLLAVCDYKQVEVTVHKGDECQL
                     VGPAQPSHWKVLSSSGSEAAVPSVCFLVPPPNQEAQEAVTRLEAQHQALVTLWHQLHV
                     DMKSLLAWQSLRRDVQLIRSWSLATFRTLKPEEQRQALHSLELHYQAFLRDSQDAGGF
                     GPEDRLMAEREYGSCSHHYQQLLQSLEQGAQEESRCQRCISELKDIRLQLEACETRTV
                     HRLRLPLDKEPARECAQRIAEQQKAQAEVEGLGKGVARLSAEAEKVLALPEPSPAAPT
                     LRSELELTLGKLEQVRSLSAIYLEKLKTISLVIRGTQGAEEVLRAHEEQLKEAQAVPA
                     TLPELEATKASLKKLRAQAEAQQPTFDALRDELRGAQEVGERLQQRHGERDVEVERWR
                     ERVAQLLERWQAVLAQTDVRQRELEQLGRQLRYYRESADPLGAWLQDARRRQEQIQAM
                     PLADSQAVREQLRQEQALLEEIERHGEKVEECQRFAKQYINAIKDYELQLVTYKAQLE
                     PVASPAKKPKVQSGSESVIQEYVDLRTHYSELTTLTSQYIKFISETLRRMEEEERLAE
                     QQRAEERERLAEVEAALEKQRQLAEAHAQAKAQAEREAKELQQRMQEEVVRREEAAVD
                     AQQQKRSIQEELQQLRQSSEAEIQAKARQAEAAERSRLRIEEEIRVVRLQLEATERQR
                     GGAEGELQALRARAEEAEAQKRQAQEEAERLRRQVQDESQRKRQAEVELASRVKAEAE
                     AAREKQRALQALEELRLQAEEAERRLRQAEVERARQVQVALETAQRSAEAELQSKRAS
                     FAEKTAQLERSLQEEHVAVAQLREEAERRAQQQAEAERAREEAERELERWQLKANEAL
                     RLRLQAEEVAQQKSLAQAEAEKQKEEAEREARRRGKAEEQAVRQRELAEQELEKQRQL
                     AEGTAQQRLAAEQELIRLRAETEQGEQQRQLLEEELARLQREAAAATQKRQELEAELA
                     KVRAEMEVLLASKARAEEESRSTSEKSKQRLEAEAGRFRELAEEAARLRALAEEAKRQ
                     RQLAEEDAARQRAEAERVLAEKLAAIGEATRLKTEAEIALKEKEAENERLRRLAEDEA
                     FQRRRLEEQAAQHKADIEERLAQLRKASDSELERQKGLVEDTLRQRRQVEEEILALKA
                     SFEKAAAGKAELELELGRIRSNAEDTLRSKEQAELEAARQRQLAAEEERRRREAEERV
                     QKSLAAEEEAARQRKAALEEVERLKAKVEEARRLRERAEQESARQLQLAQEAAQKRLQ
                     AEEKAHAFAVQQKEQELQQTLQQEQSVLDQLRGEAEAARRAAEEAEEARVQAEREAAQ
                     SRRQVEEAERLKQSAEEQAQARAQAQAAAEKLRKEAEQEAARRAQAEQAALRQKQAAD
                     AEMEKHKKFAEQTLRQKAQVEQELTTLRLQLEETDHQKNLLDEELQRLKAEATEAARQ
                     RSQVEEELFSVRVQMEELSKLKARIEAENRALILRDKDNTQRFLQEEAEKMKQVAEEA
                     ARLSVAAQEAARLRQLAEEDLAQQRALAEKMLKEKMQAVQEATRLKAEAELLQQQKEL
                     AQEQARRLQEDKEQMAQQLAEETQGFQRTLEAERQRQLEMSAEAERLKLRVAEMSRAQ
                     ARAEEDAQRFRKQAEEIGEKLHRTELATQEKVTLVQTLEIQRQQSDHDAERLREAIAE
                     LEREKEKLQQEAKLLQLKSEEMQTVQQEQLLQETQALQQSFLSEKDSLLQRERFIEQE
                     KAKLEQLFQDEVAKAQQLREEQQRQQQQMEQERQRLVASMEEARRRQHEAEEGVRRKQ
                     EELQQLEQQRRQQEELLAEENQRLREQLQLLEEQHRAALAHSEEVTASQVAATKTLPN
                     GRDALDGPAAEAEPEHSFDGLRRKVSAQRLQEAGILSAEELQRLAQGHTTVDELARRE
                     DVRHYLQGRSSIAGLLLKATNEKLSVYAALQRQLLSPGTALILLEAQAASGFLLDPVR
                     NRRLTVNEAVKEGVVGPELHHKLLSAERAVTGYKDPYTGQQISLFQAMQKGLIVREHG
                     IRLLEAQIATGGVIDPVHSHRVPVDVAYRRGYFDEEMNRVLADPSDDTKGFFDPNTHE
                     NLTYLQLLERCVEDPETGLCLLPLTDKAAKGGELVYTDSEARDVFEKATVSAPFGKFQ
                     GKTVTIWEIINSEYFTAEQRRDLLRQFRTGRITVEKIIKIIITVVEEQEQKGRLCFEG
                     LRSLVPAAELLESRVIDRELYQQLQRGERSVRDVAEVDTVRRALRGANVIAGVWLEEA
                     GQKLSIYNALKKDLLPSDMAVALLEAQAGTGHIIDPATSARLTVDEAVRAGLVGPEFH
                     EKLLSAEKAVTGYRDPYTGQSVSLFQALKKGLIPREQGLRLLDAQLSTGGIVDPSKSH
                     RVPLDVACARGCLDEETSRALSAPRADAKAYSDPSTGEPATYGELQQRCRPDQLTGLS
                     LLPLSEKAARARQEELYSELQARETFEKTPVEVPVGGFKGRTVTVWELISSEYFTAEQ
                     RQELLRQFRTGKVTVEKVIKILITIVEEVETLRQERLSFSGLRAPVPASELLASGVLS
                     RAQFEQLKDGKTTVKDLSELGSVRTLLQGSGCLAGIYLEDTKEKVSIYEAMRRGLLRA
                     TTAALLLEAQAATGFLVDPVRNQRLYVHEAVKAGVVGPELHEQLLSAEKAVTGYRDPY
                     SGSTISLFQAMQKGLVLRQHGIRLLEAQIATGGIIDPVHSHRVPVDVAYQRGYFSEEM
                     NRVLADPSDDTKGFFDPNTHENLTYRQLLERCVEDPETGLRLLPLKGAEKAEVVETTQ
                     VYTEEETRRAFEETQIDIPGGGSHGGSTMSLWEVMQSDLIPEEQRAQLMADFQAGRVT
                     KERMIIIIIEIIEKTEIIRQQGLASYDYVRRRLTAEDLFEARIISLETYNLLREGTRS
                     LREALEAESAWCYLYGTGSVAGVYLPGSRQTLSIYQALKKGLLSAEVARLLLEAQAAT
                     GFLLDPVKGERLTVDEAVRKGLVGPELHDRLLSAERAVTGYRDPYTEQTISLFQAMKK
                     ELIPTEEALRLLDAQLATGGIVDPRLGFHLPLEVAYQRGYLNKDTHDQLSEPSEVRSY
                     VDPSTDERLSYTQLLRRCRRDDGTGQLLLPLSDARKLTFRGLRKQITMEELVRSQVMD
                     EATALQLREGLTSIEEVTKNLQKFLEGTSCIAGVFVDATKERLSVYQAMKKGIIRPGT
                     AFELLEAQAATGYVIDPIKGLKLTVEEAVRMGIVGPEFKDKLLSAERAVTGYKDPYSG
                     KLISLFQAMKKGLILKDHGIRLLEAQIATGGIIDPEESHRLPVEVAYKRGLFDEEMNE
                     ILTDPSDDTKGFFDPNTEENLTYLQLMERCITDPQTGLCLLPLKEKKRERKTSSKSSV
                     RKRRVVIVDPETGKEMSVYEAYRKGLIDHQTYLELSEQECEWEEITISSSDGVVKSMI
                     IDRRSGRQYDIDDAIAKNLIDRSALDQYRAGTLSITEFADMLSGNAGGFRSRSSSVGS
                     SSSYPISPAVSRTQLASWSDPTEETGPVAGILDTETLEKVSITEAMHRNLVDNITGQR
                     LLEAQACTGGIIDPSTGERFPVTDAVNKGLVDKIMVDRINLAQKAFCGFEDPRTKTKM
                     SAAQALKKGWLYYEAGQRFLEVQYLTGGLIEPDTPGRVPLDEALQRGTVDARTAQKLR
                     DVGAYSKYLTCPKTKLKISYKDALDRSMVEEGTGLRLLEAAAQSTKGYYSPYSVSGSG
                     STAGSRTGSRTGSRAGSRRGSFDATGSGFSMTFSSSSYSSSGYGRRYASGSSASLGGP
                     ESAVA"
     exon            124..185
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            186..275
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            276..353
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            354..446
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            447..613
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            614..729
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            730..836
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            837..956
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            957..1052
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1053..1180
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..1274
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1275..1429
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1430..1748
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1749..1826
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1827..1988
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1989..2093
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2094..2189
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2190..2315
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2316..2468
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2469..2623
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2624..2750
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2751..2934
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2935..3092
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3093..3271
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3272..3410
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3411..3767
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3768..3851
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3852..3956
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3957..4055
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            4056..7436
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            7437..14679
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      14659..14664
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /note="hexamer: AATAAA"
     polyA_site      14679
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /note="major polyA site"
ORIGIN      
        1 cccacccgac gctgccgcca gtggcttgtg cctcctccga ggggcctgtg acctcccaca
       61 cccctggccc gctccgtctg ccccgtgggc tcctgccacc gtccccgatg aagatcgtgc
      121 ccgatgagcg ggatcgtgtg cagaagaaaa ccttcaccaa gtgggtcaac aagcacctca
      181 tcaaggccca gaggcacatc agtgacctgt atgaagacct ccgcgatggc cacaacctca
      241 tctccctgct ggaggtcctc tcgggggaca gcctgccccg ggagaagggg aggatgcgtt
      301 tccacaagct gcagaatgtc cagattgccc tggactacct ccggcaccgc caggtgaagc
      361 tggtgaacat caggaatgat gacatcgctg acggcaaccc caagctgacc cttggcctca
      421 tctggacaat cattctgcac ttccagatct cagatatcca ggtgagtggg cagtcggagg
      481 acatgacggc caaggagaag ctgctgctgt ggtcgcagcg aatggtggag gggtaccagg
      541 gcctgcgatg cgacaacttc acctccagct ggagagacgg ccgcctcttc aatgccatca
      601 tccaccggca caagcccctg ctcatcgaca tgaacaaggt gtaccggcag accaacctgg
      661 agaacctgga ccaggccttc tctgtggcgg agcgggacct gggagtgacg cggctcctgg
      721 accctgagga cgtggatgtc cctcagcccg acgagaagtc catcatcacc tacgtctcgt
      781 cgctgtatga cgccatgccc cgcgtgccgg acgtgcagga tggggtgagg gccaacgagc
      841 tgcagctgcg ctggcaggag taccgggagc tggtgctgct gctgcttcag tggatgcgac
      901 accacacggc cgcctttgag gaacgcaggt tcccctccag cttcgaggag attgagatcc
      961 tgtggtctca gttcctgaag tttaaggaga tggagctacc agccaaggag gccgacaaga
     1021 acaggtccaa gggcatctac caatccctgg agggagcggt gcaagcaggc cagctcaagg
     1081 tgccccctgg ctaccacccg ctggatgtgg agaaggagtg gggcaagctg cacgtggcca
     1141 tcctggagcg ggagaagcag ctccgcagcg agtttgagag gctggagtgt cttcagcgca
     1201 tcgtgaccaa gctgcagatg gaggcggggc tgtgtgagga gcagctgaac caggccgacg
     1261 ccctgctgca gtcggatgtc cggctgctgg ctgcaggcaa agtgccacag cgggcggggg
     1321 aggtggaacg ggacttggac aaggcggata gcatgatccg gctgctcttc aacgacgtgc
     1381 agaccctcaa ggatggacgg cacccgcagg gcgagcagat gtaccgcagg gtgtaccgtc
     1441 tgcacgagcg cctggtagcc atccgcaccg agtacaacct acggctgaag gcaggcgtgg
     1501 cggcccctgc aacccaggtg gcccaggtga ctctgcagag tgtgcagagg cgccccgagc
     1561 tggaggactc cactctgcgc tacctgcagg acctgctggc ctgggtggag gagaaccagc
     1621 accgtgtgga tggcgctgag tggggtgtgg acctgcccag cgtggaggcg cagctgggca
     1681 gccaccgagg cctgcaccag tccatcgaag aattccgggc caagatcgag cgggcacgga
     1741 gtgacgaggg ccagctctcc cccgccaccc ggggtgccta ccgtgactgc ctgggtcggc
     1801 tggacctgca gtacgccaag ctgctgaact cctccaaggc ccgcctcagg tccctggaga
     1861 gcttgcacag ctttgtggca gccgccacta aggagctaat gtggctgaat gagaaggagg
     1921 aggaggaggt gggcttcgac tggagcgacc gcaacaccaa catgaccgcc aagaaggaga
     1981 gctactcggc gctgatgcgg gagctggagc tgaaggagaa gaagatcaag gagctccaaa
     2041 atgctgggga ccggctgctg cgggaggacc acccggcccg gcccacggtg gagtccttcc
     2101 aggcggccct gcagacgcag tggagctgga tgctacagct gtgctgctgt atcgaggcac
     2161 acctgaagga gaacgctgcc tactttcagt tcttctcaga tgtgcgggag gccgaggggc
     2221 agttgcagaa gctgcaggag gcactgcgta ggaaatacag ttgtgatcgc tccgccaccg
     2281 tcacccggct ggaggacctg ctgcaggatg cccaggacga gaaggaacag ctgaacgagt
     2341 acaagggcca cctctcaggc ctggccaagc gggccaaggc cgtcgtgcag ctgaagcccc
     2401 gccacccagc ccaccccatg cggggccgcc tgcccctgct ggccgtgtgc gactataagc
     2461 aggtggaggt gactgtgcac aagggtgacg agtgccagct ggtgggccct gcacagccgt
     2521 cccactggaa ggtgctcagc agctccggca gcgaggccgc cgtgccctcc gtgtgcttcc
     2581 tggtgccccc gcccaaccag gaggcccagg aggccgtcac caggctggag gcccagcacc
     2641 aggccctggt cacgctgtgg caccagttgc acgtggacat gaagagcctt ctggcctggc
     2701 agagccttcg ccgcgacgtg cagctcatcc gctcctggtc cctggccacg ttccgcaccc
     2761 tgaagccaga ggagcagcgc caagccctgc acagcctgga gctgcactac caggccttcc
     2821 tgcgggacag ccaggacgcg ggcggcttcg gacccgagga ccggctgatg gctgagcgcg
     2881 agtacggctc ctgcagccac cactaccagc agctgctgca gagcctggaa cagggtgcac
     2941 aggaagagtc tcgctgccag cgctgcatct ccgagctcaa agacatccgg ctgcagctgg
     3001 aggcctgtga gacgcgcacc gtgcaccgcc tgcggctgcc gctggacaaa gagccggcac
     3061 gggagtgtgc ccagcgcatc gccgagcagc agaaggcaca ggcagaggtg gaggggctgg
     3121 gcaagggggt cgcccggctc tctgccgagg ccgagaaggt cttggcccta ccagagccat
     3181 cgcctgcggc ccccacgctg cgctcggagc tggagctgac gctgggcaag ctggagcagg
     3241 tccgcagcct gtctgccatc tacctggaga agctcaagac catcagcctg gtgatccgcg
     3301 gcacgcaggg ggccgaggag gtgctcaggg cccacgagga gcagctcaag gaggcccagg
     3361 ccgtgccggc caccctcccg gagctcgagg ccaccaaggc ctctctgaag aagctgcggg
     3421 cccaggccga ggcacagcag cccacgttcg acgccctgcg ggatgagctg cggggggcac
     3481 aggaggtggg ggagcgactg cagcagcggc acggggagcg ggacgtggag gtggagcgct
     3541 ggcgggagcg ggtcgcccag ttgcttgagc gctggcaggc tgtgctggcc cagaccgacg
     3601 tgcggcagcg cgagctcgag caactgggcc gccagctgcg ttactaccgc gagagtgcag
     3661 accccttggg cgcctggctg caggacgcca ggcggcggca ggagcagatc caggccatgc
     3721 cgctggccga cagccaggct gtgcgggagc agctgcggca ggagcaggcc ctgctggagg
     3781 agatcgagcg ccacggcgag aaggtcgagg agtgccagag gtttgcgaaa cagtacatca
     3841 acgccatcaa ggactatgaa ctccagctgg tgacgtacaa ggcgcagctt gagccggtgg
     3901 cctccccggc caagaagccc aaggtccagt cgggatcaga gagtgtcatc caggagtacg
     3961 tggacctgcg tacgcactac agcgagctga ccacactgac gagccagtac atcaagttca
     4021 tcagcgagac tctgcggcgc atggaggagg aggagaggct ggctgagcag cagcgggcag
     4081 aggagcgcga gcggctggcc gaggtggagg ccgcgctgga gaagcagcgg cagctggccg
     4141 aggcgcacgc ccaggcaaag gcacaggcgg agcgggaggc gaaggagctg cagcagcgca
     4201 tgcaggagga ggtggtgcgg cgggaggagg cggcggtgga cgcgcagcag cagaagcgca
     4261 gcattcagga ggagctgcag cagctgcggc agagctcgga ggcggagatc caggccaagg
     4321 cccggcaggc agaggcggct gagcgcagcc ggctgcgcat cgaggaggag atccgcgtgg
     4381 tgcgcctgca gttggaggcc accgagcgcc agcgtggcgg ggctgagggg gagctgcagg
     4441 cactgcgtgc acgggcggag gaggctgagg cacaaaagcg acaggcgcag gaggaggccg
     4501 agcgcttgcg gaggcaggtg caggacgaga gccagcgtaa gcggcaggcg gaggtggagc
     4561 tggcctcgcg cgtgaaggcc gaggccgagg cggcgcgcga gaagcagcgg gccctgcagg
     4621 ccctggagga gctgcggctg caggcggagg aggcggagcg gcgcctgcgg caggccgagg
     4681 tggagcgagc gcggcaggta caggtggccc tggagacggc gcagcgcagt gcagaggcgg
     4741 agctgcagag caaacgcgcc tccttcgccg agaagacggc acagctggag cgctccctgc
     4801 aggaggaaca cgtggctgtg gcacagctgc gggaggaggc tgagcggcgg gcacagcagc
     4861 aggccgaggc cgagcgggcg cgcgaggagg cagagcggga gctggagcgc tggcagctca
     4921 aggccaacga ggcgctacgg ctgcggctgc aggcggagga ggtggcgcag cagaagagcc
     4981 tggcgcaggc cgaggctgag aagcagaagg aggaggcgga gcgcgaggcg cggcggcgcg
     5041 gcaaggcgga ggagcaggcc gtccggcagc gggagctggc tgaacaagag ctggagaagc
     5101 agcggcagct ggcggaaggc accgcgcagc agcgcctggc cgcggagcag gagttgatcc
     5161 ggctgcgggc cgagacggag cagggggagc agcagcggca gctgctggag gaggagctgg
     5221 cccggctgca gcgtgaggcg gctgcagcca cgcagaaacg gcaggagctg gaagccgagc
     5281 tggccaaggt gcgggccgag atggaggtgc tgctggccag caaggcgagg gctgaggagg
     5341 agtcgcgctc caccagcgag aagtccaagc agaggctgga ggccgaggcc ggccggttcc
     5401 gcgagctggc cgaggaggcc gcccgcctgc gtgccctggc ggaagaggcc aagcggcagc
     5461 ggcagctggc cgaggaagac gcggcgcggc agcgggccga ggcggagcgg gtgcttgcgg
     5521 agaagctggc cgccatcggc gaggccacgc ggctcaagac ggaggcggag atcgcgctca
     5581 aggagaagga ggcggagaac gagcgcctgc ggcggctggc ggaggacgag gccttccagc
     5641 ggcggcggct ggaggagcag gccgcgcaac acaaggctga catcgaggag cgcctggccc
     5701 agctgcgcaa ggcatcggac agcgagctgg agcggcagaa ggggctggtg gaggacacgc
     5761 tgaggcagcg gcggcaggtg gaggaggaga tcctggcgct gaaggcgagc ttcgagaagg
     5821 cggccgctgg caaggcggag ctggagctgg agctgggacg catccgcagc aacgcggagg
     5881 acacgctgcg cagcaaggag caggccgagc tggaggctgc gaggcagcgg cagctggcgg
     5941 cggaggagga gcggcggcgc cgtgaggctg aggagcgcgt gcagaagagc ctggcggccg
     6001 aggaggaggc cgcacggcag cggaaggcgg cgctggagga agtcgagcgg ctgaaagcca
     6061 aggtggagga ggcgcggcgc ctgcgggagc gagcggagca ggagtcggcg cggcagctgc
     6121 agctggccca ggaggccgcc cagaagcggc tgcaggcgga agagaaggca cacgccttcg
     6181 cggtgcagca gaaggagcag gagctacagc agacgctgca gcaggagcag agcgtgctgg
     6241 accagctgcg cggcgaggcg gaggcggccc ggcgggcggc tgaggaggcg gaggaggccc
     6301 gggtgcaggc ggagcgtgag gcggcgcagt cccggcggca ggtggaagag gccgagcggc
     6361 tgaagcagtc ggcagaggag caggcacagg cccgggctca ggcacaggcg gctgcagaga
     6421 agctgcgcaa ggaggccgag caagaggcgg cgcggcgggc acaggcggag caggcggccc
     6481 tgcggcagaa gcaggcagct gacgcggaga tggagaagca taagaaattc gccgagcaga
     6541 cgctgcggca gaaggcgcag gtggagcagg agctgacaac actgcggctg cagctggagg
     6601 agaccgacca ccagaagaac ctgctggacg aggagctgca gcggctgaag gcggaggcca
     6661 cggaggccgc acgccagcgc agccaggtgg aggaggagct cttctcggtg cgcgtgcaga
     6721 tggaggagct gagcaagctc aaggcacgca tcgaggctga gaaccgcgca ctcatcttgc
     6781 gtgacaagga caatacgcag cgcttcctgc aggaggaggc tgagaagatg aagcaggtgg
     6841 cggaggaggc cgcgcggctg agtgtggcgg cccaagaggc tgcgcgactg cggcagctgg
     6901 cagaggagga cctggcacag cagcgggcct tggcagagaa gatgctcaag gagaagatgc
     6961 aggcggtgca ggaggccacg cgactcaagg ctgaggcgga actgctgcag cagcagaagg
     7021 agcttgcgca ggagcaggcg cggcggctgc aggaggacaa ggagcagatg gcgcagcagc
     7081 tggcggagga gacgcagggc ttccagcgga cgctggaggc cgagcggcag cggcagctgg
     7141 agatgagcgc tgaggctgag cgcctcaagc tgcgtgtggc cgagatgagc cgagcccagg
     7201 cccgcgctga ggaggacgcc cagcgcttcc ggaagcaggc ggaggagatc ggtgagaagc
     7261 tgcaccgcac ggagctcgcc acccaggaga aggtgaccct ggtgcagaca ctggagatcc
     7321 agcgacagca gagtgaccat gatgccgagc gcctgcggga ggccatcgct gagctggagc
     7381 gtgagaagga gaagctccaa caggaggcca aactgctgca gctcaagtct gaggagatgc
     7441 agacggtgca gcaggagcag ctgctgcagg agacgcaggc cctgcagcaa agcttcctct
     7501 ctgaaaagga cagcctgcta cagcgggagc gcttcatcga gcaggagaag gccaagctgg
     7561 agcagctctt ccaggacgag gtggccaagg cacagcagct gcgtgaggag cagcagcggc
     7621 agcagcagca gatggagcag gaacggcagc ggctggtggc cagcatggag gaggcgcggc
     7681 ggcggcagca tgaggccgag gagggcgtgc ggcgcaagca ggaggagctg cagcagctgg
     7741 agcagcagcg gcggcagcag gaggagctgc tggctgagga gaaccagagg ctgcgtgagc
     7801 agctgcagct cctggaggag cagcaccggg ccgcgctggc gcactcagag gaggtcactg
     7861 cctcgcaggt ggctgccaca aagaccctgc ccaatggccg ggatgcactt gatggccccg
     7921 cggcagaggc agagccggag cacagcttcg atggcctgcg gcggaaggtg tcagctcaga
     7981 ggctgcagga ggccggcatc ctgagtgcgg aggagctgca gcggttggcg cagggccaca
     8041 ccacggtgga cgagctcgca cggcgggaag acgtgcgcca ctacctgcag ggccgcagca
     8101 gtatcgcagg gctgttgctg aaggccacca atgagaagct gagtgtttac gccgccctgc
     8161 agaggcagct gctgagtccc ggcacggccc tcatcctgct ggaggcgcag gcggcctcag
     8221 gcttcctgct ggaccctgtg cggaaccggc ggctgaccgt caacgaggct gtgaaggagg
     8281 gtgtggtggg ccccgagctg caccacaagc tgctgtcggc cgagcgcgcc gtcactggct
     8341 acaaggaccc ctacactggc cagcagatct ctctcttcca agccatgcag aagggcctca
     8401 tcgtccggga gcacggcatc cgcctgctgg aggcccagat cgccacgggc ggcgttatcg
     8461 accccgtgca cagccaccgc gtgcccgtgg acgtggccta ccggcgcggc tacttcgacg
     8521 aggagatgaa ccgcgtcctg gcggacccca gcgacgacac caagggcttc tttgacccca
     8581 acacgcacga gaacctcacg tacctgcagc tactggagcg ctgcgtggag gaccccgaga
     8641 cgggcctgtg ccttctgcca ctcacggata aggctgccaa gggcggggag ctggtctaca
     8701 ctgactccga ggcccgggac gtctttgaga aggccaccgt gtctgcgccg ttcggcaagt
     8761 tccagggcaa gacggtgacc atttgggaga tcatcaactc ggaatacttc acggcagagc
     8821 agcggcggga cctgctgcgg cagttccgca cgggccggat cacagtggag aagatcatca
     8881 agatcatcat cacggtggtg gaggagcagg agcagaaggg ccggctttgc tttgagggcc
     8941 tgcgcagcct ggtgccagcc gccgagctgc tggagagcag ggtcatcgac cgcgagctct
     9001 accagcagct gcagcgaggt gagcgctctg tgcgagacgt agccgaggtg gacactgtgc
     9061 ggcgggctct ccggggtgcc aacgtcatcg cgggtgtatg gctggaggag gcggggcaga
     9121 agctgagtat ctacaatgcc ctgaagaaag acctgctgcc atccgacatg gccgtggccc
     9181 tgttggaagc ccaggccggc accgggcaca tcatcgaccc cgccaccagc gcccggctga
     9241 ccgtggacga ggcagtgcgt gctggcctgg tgggccccga gtttcatgag aagctgctat
     9301 cagccgagaa ggctgtgaca gggtacaggg acccctacac agggcagagc gtctccctgt
     9361 tccaggccct gaagaagggc ctcattcccc gggagcaggg cctgcgcctg ttggacgccc
     9421 agctgtccac gggcggcatc gtggacccca gcaagagcca ccgcgtgccc ctggatgtcg
     9481 cctgcgcccg aggctgcctg gatgaggaga ccagcagggc cctgtcggca ccaagggccg
     9541 acgccaaggc ctacagtgac cccagcacag gggagccggc cacctacggc gagctccagc
     9601 agcggtgccg gcccgaccag ctgaccgggc tgagcctgct gccgctctca gaaaaggctg
     9661 ctcgggcccg gcaggaggag ctctactcag agctgcaggc ccgtgagacc tttgaaaaga
     9721 ccccggttga ggtccccgtg ggtggcttca agggcaggac ggtgacggtg tgggagctca
     9781 tcagctctga gtacttcact gcggagcagc ggcaggagct gttgcgtcag ttccgcacgg
     9841 gcaaggtcac cgtggagaag gtcatcaaga ttctcattac catcgtggag gaggtggaga
     9901 ccctgcggca ggagaggctg tccttcagcg gcctccgtgc ccctgtgcca gccagcgagc
     9961 tcctggcttc cggggtcctc agcagagccc agtttgagca gctcaaggac ggcaagacga
    10021 cggtcaagga cctttcggag ctgggctccg tgcggacgct gctgcagggc agtggctgcc
    10081 tcgccggcat ctacctggag gacaccaagg agaaggtgtc catctacgag gccatgcgcc
    10141 ggggcctgct gagagccaca acggctgcgc tcctgctgga ggcgcaggcg gccactggct
    10201 tcctggtgga ccccgtgcgg aaccagcgcc tgtatgtcca cgaggccgtg aaggcgggcg
    10261 tggtgggccc cgagcttcac gagcagctgc tgtctgccga gaaggccgtc accggctaca
    10321 gagaccccta ctcgggcagc accatctccc tcttccaggc catgcagaag ggcctggttc
    10381 tccggcagca cggcatccgc ctgctggagg cccagatcgc cacgggcggc atcatcgacc
    10441 ccgtgcacag ccaccgcgtg cctgtggacg tggcctacca gcgcggctac ttcagtgagg
    10501 agatgaaccg cgtcctggcg gaccccagcg acgacaccaa gggcttcttt gaccccaaca
    10561 cgcatgagaa cctcacgtac aggcagctgc tggagcggtg cgtggaggac cccgagacgg
    10621 gcttgcgcct tctgccactg aaaggggcgg agaaggctga ggtggtggag accacgcagg
    10681 tgtacactga ggaggagaca agaagggcat ttgaagagac acagatcgac attcccggcg
    10741 gcggcagcca cggcggctcc accatgtccc tgtgggaggt gatgcagtcg gacctgatcc
    10801 ccgaggagca gcgggcccag ctgatggctg acttccaggc cggccgggtg accaaggaac
    10861 gcatgatcat catcatcatc gagatcattg agaagacaga gatcatccgc cagcagggtc
    10921 tggcctccta cgactacgtg cgccgccgcc tcacggctga ggacctgttc gaggctcgga
    10981 tcatctctct cgagacctac aacctgctcc gggagggcac caggagcctc cgtgaggctc
    11041 tcgaggcgga gtccgcctgg tgctacctct atggcacggg ctccgtggct ggtgtctacc
    11101 tgcccggttc caggcagaca ctgagcatct accaggctct caagaaaggg ctgctgagtg
    11161 ccgaggtggc ccgcctgctg ctggaggcac aggcagccac aggcttcctg ctggacccgg
    11221 tgaaggggga gcggctgact gtggatgagg ctgtgcggaa gggcctcgtg gggcccgagc
    11281 tgcacgaccg cctgctctcg gctgagcggg cggtcaccgg ctaccgtgac ccctacaccg
    11341 agcagaccat ctcgctcttc caggccatga agaaggagct gatccctact gaggaggccc
    11401 tgcggctgct ggatgcccag ctggccaccg gcggcatcgt ggacccccgc ctgggcttcc
    11461 accttcccct ggaggtggct taccagcgtg gctacctcaa caaggacacg cacgaccagc
    11521 tgtcagagcc cagcgaggtg cgcagctacg tggacccgtc caccgacgag cgcctcagct
    11581 acacgcagct gctcaggcgg tgccgtcgtg acgacggcac cggccagctg ctcctgccac
    11641 tgtcggacgc ccgcaagctg accttccgtg gcctgcggaa gcagatcacc atggaggagc
    11701 tggtgcgctc gcaggtcatg gacgaggcca cggcgctgca gctgcgggag ggcctgacct
    11761 ccatcgagga ggtcaccaag aacttgcaga agttcctgga aggcaccagc tgcatcgctg
    11821 gtgtcttcgt ggacgccacc aaggaacggc tctcggtgta ccaggccatg aagaagggca
    11881 tcatccgccc cggcacagcc tttgagctcc tggaggcgca ggcggccacc ggttacgtca
    11941 tcgaccccat caagggactg aagctgacgg tggaggaggc tgtgcgtatg ggcattgtgg
    12001 gccccgagtt caaggacaag ctgctgtcgg ccgagcgcgc cgtcactggg tacaaggacc
    12061 cctactctgg gaagctcatc tccctcttcc aggccatgaa gaagggcctg atcctgaagg
    12121 accatggcat ccgcctgctg gaggcccaga tcgccacggg cggcatcatc gaccctgagg
    12181 agagccaccg gctgcccgtg gaggtggcct acaagcgcgg cctcttcgat gaggagatga
    12241 acgagatcct gaccgacccc tcggacgaca ccaagggctt ctttgaccct aacacggagg
    12301 agaacctcac ctacctgcag ctgatggagc gttgtatcac tgacccccag acgggcctgt
    12361 gtctcttgcc gctgaaggag aagaagcggg agcggaagac gtcctccaag tcctccgtgc
    12421 gcaagcgccg agtggtcatc gtggaccccg agacgggcaa ggagatgtca gtgtacgagg
    12481 cctaccgcaa gggcctgatt gaccaccaga cgtacctgga gctgtccgag caggagtgcg
    12541 agtgggagga gatcaccatc tcctcctcgg acggcgtggt caagtccatg atcatcgacc
    12601 gccgctccgg gcgccagtac gacatcgatg atgccatcgc caagaacctc atcgaccgct
    12661 cggcactgga ccagtaccgc gccggcacgc tctccatcac cgagttcgcc gacatgctct
    12721 cgggcaacgc cggtggtttc cgctcccgtt cctcctcggt gggatcctcc tcctcctacc
    12781 ccatcagccc cgccgtctcc aggacccagc tggcctcctg gtcagacccc actgaggaga
    12841 cgggccccgt ggctggcatc ctggacacgg agacgctgga gaaggtgtcc atcaccgagg
    12901 ccatgcaccg gaacctggtg gataacatca cggggcagcg gctgctggag gcgcaggcct
    12961 gcaccggggg catcatcgac cccagcaccg gtgagcgctt ccctgtcacc gacgccgtca
    13021 acaagggcct ggtggacaag atcatggtgg accgcatcaa cctggcccag aaggccttct
    13081 gcggcttcga ggacccacgc accaagacca agatgtcggc cgcccaggcc ctgaagaagg
    13141 gctggctcta ctacgaggcc ggccagcgct tcctggaggt gcagtacctg accggcggct
    13201 tgatcgagcc cgacacgccg ggccgcgtgc ccctggacga ggccctgcag cgcggcacgg
    13261 tggacgcccg caccgcacag aagctgcgtg acgtgggcgc ctactccaag tacctcacct
    13321 gccctaagac caagctcaag atctcctata aggacgcgct ggaccgcagc atggtggagg
    13381 agggcacggg gctgcggctg ctggaggctg ccgcgcagtc caccaagggc tactacagcc
    13441 cctacagcgt cagcggctcc ggctctaccg ctggctcccg caccggctcg cgcaccggct
    13501 cccgggccgg ctcccgccgc ggcagctttg acgccaccgg ctccggcttc tccatgacct
    13561 tctcttcatc ctcctactcc tcctcgggct acggccgccg ctacgcctcg gggtcctcgg
    13621 cctccctggg gggccctgag tctgccgtgg cctgaggctg cctgcgccca ccccgctctg
    13681 catgcggccc agcccggctc ccaccgaggc gcgggggccg ttttcaacgc ttaaaggtgt
    13741 cttcctccca agtggtgcct aaagtttaac caaaaagacc agactaatat attaatatat
    13801 atctgctgtc cagacagcct gtatcttggg ggacagggct ggcccagccc tgctggccgc
    13861 ctcaccccct cgggtctcct cactcccttc tacctgccac tcacacagcc aggtgccttg
    13921 gagggtccca agctgggccc cagcccaccc tcctgtcttc ccagggtagc ccgcctgcca
    13981 gtcctagctg cacagggcag ctgggcccaa ccctgtctgt agagggccct ggtgtttcta
    14041 gcactggcct gcacggtggg ccttgctggg gacggggggc cccagtcagc ctctctccca
    14101 gtctacccag agaagcccct tccccatggg aagacgaggc cctcgggccc agcccccaca
    14161 gtgctgtctg atctgtgctt tccagctcac cccccacact cactcctgag acccctggcc
    14221 tccggcgtca gcctccagcc tctgttcccc tagtaagtgc cttccatgtc ggcctctaac
    14281 cccaggcccc gaggacccag acccagtggg gaggcggacg ttccagccgg catggctggg
    14341 aactgcagac ctgtcctcct ggtgggtcca ggggcccctc cagcttgtgg agccccacac
    14401 tggggtgccg cctgcccgtc tctctcccat ggagccccag ccccctttgg gcccagggac
    14461 accagccagg ctctgtgctg accctcctgt tgcacccagc cctggtctca gcagcgacca
    14521 cccctgcctc caccctctga gctttgcatg ttccactaac cccgggcggg tggcaggtgg
    14581 aggtgtcagg ctgctggcgc ctctgcaagg gcagaacact aacctgaccg tgggcggggc
    14641 cttgcggtat ccgcccccaa taaaagcaat tccaacctt
//
