LOCUS       NM_201378              14683 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens plectin (PLEC), transcript variant 2, mRNA.
ACCESSION   NM_201378
VERSION     NM_201378.4
KEYWORDS    RefSeq; MANE Plus Clinical.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 14683)
  AUTHORS   Rezniczek,G.A., Walko,G. and Wiche,G.
  TITLE     Plectin gene defects lead to various forms of epidermolysis bullosa
            simplex
  JOURNAL   Dermatol Clin 28 (1), 33-41 (2010)
   PUBMED   19945614
  REMARK    Review article
            Erratum:[Dermatol Clin. 2010 Apr;28(2):439-41]
REFERENCE   2  (bases 1 to 14683)
  AUTHORS   Winter,L., Abrahamsberg,C. and Wiche,G.
  TITLE     Plectin isoform 1b mediates mitochondrion-intermediate filament
            network linkage and controls organelle shape
  JOURNAL   J Cell Biol 181 (6), 903-911 (2008)
   PUBMED   18541706
REFERENCE   3  (bases 1 to 14683)
  AUTHORS   Sonnenberg,A. and Liem,R.K.
  TITLE     Plakins in development and disease
  JOURNAL   Exp Cell Res 313 (10), 2189-2203 (2007)
   PUBMED   17499243
  REMARK    Review article
REFERENCE   4  (bases 1 to 14683)
  AUTHORS   Rezniczek,G.A., Konieczny,P., Nikolic,B., Reipert,S., Schneller,D.,
            Abrahamsberg,C., Davies,K.E., Winder,S.J. and Wiche,G.
  TITLE     Plectin 1f scaffolding at the sarcolemma of dystrophic (mdx) muscle
            fibers through multiple interactions with beta-dystroglycan
  JOURNAL   J Cell Biol 176 (7), 965-977 (2007)
   PUBMED   17389230
REFERENCE   5  (bases 1 to 14683)
  AUTHORS   Pfendner,E., Rouan,F. and Uitto,J.
  TITLE     Progress in epidermolysis bullosa: the phenotypic spectrum of
            plectin mutations
  JOURNAL   Exp Dermatol 14 (4), 241-249 (2005)
   PUBMED   15810881
  REMARK    GeneRIF: discussion of phenotypic spectrum of plectin mutations
            [review]
            Review article
REFERENCE   6  (bases 1 to 14683)
  AUTHORS   Zhang,T., Haws,P. and Wu,Q.
  TITLE     Multiple variable first exons: a mechanism for cell- and
            tissue-specific gene regulation
  JOURNAL   Genome Res 14 (1), 79-89 (2004)
   PUBMED   14672974
REFERENCE   7  (bases 1 to 14683)
  AUTHORS   Rezniczek,G.A., Abrahamsberg,C., Fuchs,P., Spazierer,D. and
            Wiche,G.
  TITLE     Plectin 5'-transcript diversity: short alternative sequences
            determine stability of gene products, initiation of translation and
            subcellular localization of isoforms
  JOURNAL   Hum Mol Genet 12 (23), 3181-3194 (2003)
   PUBMED   14559777
REFERENCE   8  (bases 1 to 14683)
  AUTHORS   Andra,K., Kornacker,I., Jorgl,A., Zorer,M., Spazierer,D., Fuchs,P.,
            Fischer,I. and Wiche,G.
  TITLE     Plectin-isoform-specific rescue of hemidesmosomal defects in
            plectin (-/-) keratinocytes
  JOURNAL   J Invest Dermatol 120 (2), 189-197 (2003)
   PUBMED   12542521
REFERENCE   9  (bases 1 to 14683)
  AUTHORS   Leung,C.L., Green,K.J. and Liem,R.K.
  TITLE     Plakins: a family of versatile cytolinker proteins
  JOURNAL   Trends Cell Biol 12 (1), 37-45 (2002)
   PUBMED   11854008
  REMARK    Review article
REFERENCE   10 (bases 1 to 14683)
  AUTHORS   Brown,M.J., Hallam,J.A., Liu,Y., Yamada,K.M. and Shaw,S.
  TITLE     Cutting edge: integration of human T lymphocyte cytoskeleton by the
            cytolinker plectin
  JOURNAL   J Immunol 167 (2), 641-645 (2001)
   PUBMED   11441066
  REMARK    GeneRIF: During polarization of peripheral blood T lymphocytes,
            plectin redistributes to the uropod associated with vimentin and
            fodrin. This vimentin-plectin-fodrin complex provides a continuous
            linkage from the nucleus (lamin B) to the cortical cytoskeleton.
REFERENCE   11 (bases 1 to 14683)
  AUTHORS   Schroder,R., Furst,D.O., Klasen,C., Reimann,J., Herrmann,H. and van
            der Ven,P.F.
  TITLE     Association of plectin with Z-discs is a prerequisite for the
            formation of the intermyofibrillar desmin cytoskeleton
  JOURNAL   Lab Invest 80 (4), 455-464 (2000)
   PUBMED   10780662
REFERENCE   12 (bases 1 to 14683)
  AUTHORS   Fuchs,P., Zorer,M., Rezniczek,G.A., Spazierer,D., Oehler,S.,
            Castanon,M.J., Hauptmann,R. and Wiche,G.
  TITLE     Unusual 5' transcript complexity of plectin isoforms: novel
            tissue-specific exons modulate actin binding activity
  JOURNAL   Hum Mol Genet 8 (13), 2461-2472 (1999)
   PUBMED   10556294
REFERENCE   13 (bases 1 to 14683)
  AUTHORS   Wiche,G.
  TITLE     Role of plectin in cytoskeleton organization and dynamics
  JOURNAL   J Cell Sci 111 (Pt 17), 2477-2486 (1998)
   PUBMED   9701547
  REMARK    Review article
REFERENCE   14 (bases 1 to 14683)
  AUTHORS   Elliott,C.E., Becker,B., Oehler,S., Castanon,M.J., Hauptmann,R. and
            Wiche,G.
  TITLE     Plectin transcript diversity: identification and tissue
            distribution of variants with distinct first coding exons and
            rodless isoforms
  JOURNAL   Genomics 42 (1), 115-125 (1997)
   PUBMED   9177781
REFERENCE   15 (bases 1 to 14683)
  AUTHORS   Chavanas,S., Pulkkinen,L., Gache,Y., Smith,F.J., McLean,W.H.,
            Uitto,J., Ortonne,J.P. and Meneguzzi,G.
  TITLE     A homozygous nonsense mutation in the PLEC1 gene in patients with
            epidermolysis bullosa simplex with muscular dystrophy
  JOURNAL   J Clin Invest 98 (10), 2196-2200 (1996)
   PUBMED   8941634
REFERENCE   16 (bases 1 to 14683)
  AUTHORS   Pulkkinen,L., Smith,F.J., Shimizu,H., Murata,S., Yaoita,H.,
            Hachisuka,H., Nishikawa,T., McLean,W.H. and Uitto,J.
  TITLE     Homozygous deletion mutations in the plectin gene (PLEC1) in
            patients with epidermolysis bullosa simplex associated with
            late-onset muscular dystrophy
  JOURNAL   Hum Mol Genet 5 (10), 1539-1546 (1996)
   PUBMED   8894687
REFERENCE   17 (bases 1 to 14683)
  AUTHORS   Smith,F.J., Eady,R.A., Leigh,I.M., McMillan,J.R., Rugg,E.L.,
            Kelsell,D.P., Bryant,S.P., Spurr,N.K., Geddes,J.F., Kirtschig,G.,
            Milana,G., de Bono,A.G., Owaribe,K., Wiche,G., Pulkkinen,L.,
            Uitto,J., McLean,W.H. and Lane,E.B.
  TITLE     Plectin deficiency results in muscular dystrophy with epidermolysis
            bullosa
  JOURNAL   Nat Genet 13 (4), 450-457 (1996)
   PUBMED   8696340
REFERENCE   18 (bases 1 to 14683)
  AUTHORS   McLean,W.H., Pulkkinen,L., Smith,F.J., Rugg,E.L., Lane,E.B.,
            Bullrich,F., Burgeson,R.E., Amano,S., Hudson,D.L., Owaribe,K.,
            McGrath,J.A., McMillan,J.R., Eady,R.A., Leigh,I.M., Christiano,A.M.
            and Uitto,J.
  TITLE     Loss of plectin causes epidermolysis bullosa with muscular
            dystrophy: cDNA cloning and genomic organization
  JOURNAL   Genes Dev 10 (14), 1724-1735 (1996)
   PUBMED   8698233
REFERENCE   19 (bases 1 to 14683)
  AUTHORS   Gache,Y., Chavanas,S., Lacour,J.P., Wiche,G., Owaribe,K.,
            Meneguzzi,G. and Ortonne,J.P.
  TITLE     Defective expression of plectin/HD1 in epidermolysis bullosa
            simplex with muscular dystrophy
  JOURNAL   J Clin Invest 97 (10), 2289-2298 (1996)
   PUBMED   8636409
REFERENCE   20 (bases 1 to 14683)
  AUTHORS   Liu,C.G., Maercker,C., Castanon,M.J., Hauptmann,R. and Wiche,G.
  TITLE     Human plectin: organization of the gene, sequence analysis, and
            chromosome localization (8q24)
  JOURNAL   Proc Natl Acad Sci U S A 93 (9), 4278-4283 (1996)
   PUBMED   8633055
REFERENCE   21 (bases 1 to 14683)
  AUTHORS   Pegoraro,E. and Hoffman,E.P.
  TITLE     Limb-Girdle Muscular Dystrophy Overview - RETIRED CHAPTER, FOR
            HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301582
REFERENCE   22 (bases 1 to 14683)
  AUTHORS   So,J.Y. and Teng,J.
  TITLE     Epidermolysis Bullosa Simplex
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301543
REFERENCE   23 (bases 1 to 14683)
  AUTHORS   Abicht,A., Muller,J.S. and Lochmuller,H.
  TITLE     Congenital Myasthenic Syndromes Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301347
REFERENCE   24 (bases 1 to 14683)
  AUTHORS   Lucky,A.W. and Gorell,E.
  TITLE     Epidermolysis Bullosa with Pyloric Atresia
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301336
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff in
            collaboration with Gunther Rezniczek, Gerhard Wiche. The reference
            sequence was derived from BP315878.1 and AY480045.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_201378.3.
            
            Summary: Plectin is a prominent member of an important family of
            structurally and in part functionally related proteins, termed
            plakins or cytolinkers, that are capable of interlinking different
            elements of the cytoskeleton. Plakins, with their multi-domain
            structure and enormous size, not only play crucial roles in
            maintaining cell and tissue integrity and orchestrating dynamic
            changes in cytoarchitecture and cell shape, but also serve as
            scaffolding platforms for the assembly, positioning, and regulation
            of signaling complexes (reviewed in PMID: 9701547, 11854008, and
            17499243). Plectin is expressed as several protein isoforms in a
            wide range of cell types and tissues from a single gene located on
            chromosome 8 in humans (PMID: 8633055, 8698233). Until 2010, this
            locus was named plectin 1 (symbol PLEC1 in human; Plec1 in mouse
            and rat) and the gene product had been referred to as
            'hemidesmosomal protein 1' or 'plectin 1, intermediate filament
            binding 500kDa'. These names were superseded by plectin. The
            plectin gene locus in mouse on chromosome 15 has been analyzed in
            detail (PMID: 10556294, 14559777), revealing a genomic exon-intron
            organization with well over 40 exons spanning over 62 kb and an
            unusual 5' transcript complexity of plectin isoforms. Eleven exons
            (1-1j) have been identified that alternatively splice directly into
            a common exon 2 which is the first exon to encode plectin's highly
            conserved actin binding domain (ABD). Three additional exons (-1,
            0a, and 0) splice into an alternative first coding exon (1c), and
            two additional exons (2alpha and 3alpha) are optionally spliced
            within the exons encoding the acting binding domain (exons 2-8).
            Analysis of the human locus has identified eight of the eleven
            alternative 5' exons found in mouse and rat (PMID: 14672974); exons
            1i, 1j and 1h have not been confirmed in human. Furthermore,
            isoforms lacking the central rod domain encoded by exon 31 have
            been detected in mouse (PMID:10556294), rat (PMID: 9177781), and
            human (PMID: 11441066, 10780662, 20052759). The short alternative
            amino-terminal sequences encoded by the different first exons
            direct the targeting of the various isoforms to distinct
            subcellular locations (PMID: 14559777). As the expression of
            specific plectin isoforms was found to be dependent on cell type
            (tissue) and stage of development (PMID: 10556294, 12542521,
            17389230) it appears that each cell type (tissue) contains a unique
            set (proportion and composition) of plectin isoforms, as if
            custom-made for specific requirements of the particular cells.
            Concordantly, individual isoforms were found to carry out distinct
            and specific functions (PMID: 14559777, 12542521, 18541706). In
            1996, a number of groups reported that patients suffering from
            epidermolysis bullosa simplex with muscular dystrophy (EBS-MD)
            lacked plectin expression in skin and muscle tissues due to defects
            in the plectin gene (PMID: 8698233, 8941634, 8636409, 8894687,
            8696340). Two other subtypes of plectin-related EBS have been
            described: EBS-pyloric atresia (PA) and EBS-Ogna. For reviews of
            plectin-related diseases see PMID: 15810881, 19945614. Mutations in
            the plectin gene related to human diseases should be named based on
            the position in NM_000445 (variant 1, isoform 1c), unless the
            mutation is located within one of the other alternative first
            exons, in which case the position in the respective Reference
            Sequence should be used. [provided by RefSeq, Aug 2011].
            
            Transcript Variant: This variant (2) differs in the 5' UTR and the
            5' coding region compared to variant 1. The resulting protein
            (isoform 1f) contains a distinct N-terminus compared to isoform 1c.
            Plectin isoform 1f has been found to be concentrated in
            vinculin-positive structures at actin stress fiber ends (PMID:
            14559777).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY480045.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: PMID: 18541706, 17389230
            MANE Ensembl match                 :: ENST00000356346.7/
                                                  ENSP00000348702.3
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-57                BP315878.1         2-58
            58-14683            AY480045.1         1-14626
FEATURES             Location/Qualifiers
     source          1..14683
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.3"
     gene            1..14683
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /note="plectin"
                     /db_xref="GeneID:5339"
                     /db_xref="HGNC:HGNC:9069"
                     /db_xref="MIM:601282"
     exon            1..127
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     CDS             58..13659
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /note="isoform 1f is encoded by transcript variant 2;
                     plectin 1, intermediate filament binding protein 500kDa;
                     hemidesmosomal protein 1"
                     /codon_start=1
                     /product="plectin isoform 1f"
                     /protein_id="NP_958780.1"
                     /db_xref="CCDS:CCDS43770.1"
                     /db_xref="GeneID:5339"
                     /db_xref="HGNC:HGNC:9069"
                     /db_xref="MIM:601282"
                     /translation="MAGPLPDEQDFIQAYEEVREKYKDERDRVQKKTFTKWVNKHLIK
                     AQRHISDLYEDLRDGHNLISLLEVLSGDSLPREKGRMRFHKLQNVQIALDYLRHRQVK
                     LVNIRNDDIADGNPKLTLGLIWTIILHFQISDIQVSGQSEDMTAKEKLLLWSQRMVEG
                     YQGLRCDNFTSSWRDGRLFNAIIHRHKPLLIDMNKVYRQTNLENLDQAFSVAERDLGV
                     TRLLDPEDVDVPQPDEKSIITYVSSLYDAMPRVPDVQDGVRANELQLRWQEYRELVLL
                     LLQWMRHHTAAFEERRFPSSFEEIEILWSQFLKFKEMELPAKEADKNRSKGIYQSLEG
                     AVQAGQLKVPPGYHPLDVEKEWGKLHVAILEREKQLRSEFERLECLQRIVTKLQMEAG
                     LCEEQLNQADALLQSDVRLLAAGKVPQRAGEVERDLDKADSMIRLLFNDVQTLKDGRH
                     PQGEQMYRRVYRLHERLVAIRTEYNLRLKAGVAAPATQVAQVTLQSVQRRPELEDSTL
                     RYLQDLLAWVEENQHRVDGAEWGVDLPSVEAQLGSHRGLHQSIEEFRAKIERARSDEG
                     QLSPATRGAYRDCLGRLDLQYAKLLNSSKARLRSLESLHSFVAAATKELMWLNEKEEE
                     EVGFDWSDRNTNMTAKKESYSALMRELELKEKKIKELQNAGDRLLREDHPARPTVESF
                     QAALQTQWSWMLQLCCCIEAHLKENAAYFQFFSDVREAEGQLQKLQEALRRKYSCDRS
                     ATVTRLEDLLQDAQDEKEQLNEYKGHLSGLAKRAKAVVQLKPRHPAHPMRGRLPLLAV
                     CDYKQVEVTVHKGDECQLVGPAQPSHWKVLSSSGSEAAVPSVCFLVPPPNQEAQEAVT
                     RLEAQHQALVTLWHQLHVDMKSLLAWQSLRRDVQLIRSWSLATFRTLKPEEQRQALHS
                     LELHYQAFLRDSQDAGGFGPEDRLMAEREYGSCSHHYQQLLQSLEQGAQEESRCQRCI
                     SELKDIRLQLEACETRTVHRLRLPLDKEPARECAQRIAEQQKAQAEVEGLGKGVARLS
                     AEAEKVLALPEPSPAAPTLRSELELTLGKLEQVRSLSAIYLEKLKTISLVIRGTQGAE
                     EVLRAHEEQLKEAQAVPATLPELEATKASLKKLRAQAEAQQPTFDALRDELRGAQEVG
                     ERLQQRHGERDVEVERWRERVAQLLERWQAVLAQTDVRQRELEQLGRQLRYYRESADP
                     LGAWLQDARRRQEQIQAMPLADSQAVREQLRQEQALLEEIERHGEKVEECQRFAKQYI
                     NAIKDYELQLVTYKAQLEPVASPAKKPKVQSGSESVIQEYVDLRTHYSELTTLTSQYI
                     KFISETLRRMEEEERLAEQQRAEERERLAEVEAALEKQRQLAEAHAQAKAQAEREAKE
                     LQQRMQEEVVRREEAAVDAQQQKRSIQEELQQLRQSSEAEIQAKARQAEAAERSRLRI
                     EEEIRVVRLQLEATERQRGGAEGELQALRARAEEAEAQKRQAQEEAERLRRQVQDESQ
                     RKRQAEVELASRVKAEAEAAREKQRALQALEELRLQAEEAERRLRQAEVERARQVQVA
                     LETAQRSAEAELQSKRASFAEKTAQLERSLQEEHVAVAQLREEAERRAQQQAEAERAR
                     EEAERELERWQLKANEALRLRLQAEEVAQQKSLAQAEAEKQKEEAEREARRRGKAEEQ
                     AVRQRELAEQELEKQRQLAEGTAQQRLAAEQELIRLRAETEQGEQQRQLLEEELARLQ
                     REAAAATQKRQELEAELAKVRAEMEVLLASKARAEEESRSTSEKSKQRLEAEAGRFRE
                     LAEEAARLRALAEEAKRQRQLAEEDAARQRAEAERVLAEKLAAIGEATRLKTEAEIAL
                     KEKEAENERLRRLAEDEAFQRRRLEEQAAQHKADIEERLAQLRKASDSELERQKGLVE
                     DTLRQRRQVEEEILALKASFEKAAAGKAELELELGRIRSNAEDTLRSKEQAELEAARQ
                     RQLAAEEERRRREAEERVQKSLAAEEEAARQRKAALEEVERLKAKVEEARRLRERAEQ
                     ESARQLQLAQEAAQKRLQAEEKAHAFAVQQKEQELQQTLQQEQSVLDQLRGEAEAARR
                     AAEEAEEARVQAEREAAQSRRQVEEAERLKQSAEEQAQARAQAQAAAEKLRKEAEQEA
                     ARRAQAEQAALRQKQAADAEMEKHKKFAEQTLRQKAQVEQELTTLRLQLEETDHQKNL
                     LDEELQRLKAEATEAARQRSQVEEELFSVRVQMEELSKLKARIEAENRALILRDKDNT
                     QRFLQEEAEKMKQVAEEAARLSVAAQEAARLRQLAEEDLAQQRALAEKMLKEKMQAVQ
                     EATRLKAEAELLQQQKELAQEQARRLQEDKEQMAQQLAEETQGFQRTLEAERQRQLEM
                     SAEAERLKLRVAEMSRAQARAEEDAQRFRKQAEEIGEKLHRTELATQEKVTLVQTLEI
                     QRQQSDHDAERLREAIAELEREKEKLQQEAKLLQLKSEEMQTVQQEQLLQETQALQQS
                     FLSEKDSLLQRERFIEQEKAKLEQLFQDEVAKAQQLREEQQRQQQQMEQERQRLVASM
                     EEARRRQHEAEEGVRRKQEELQQLEQQRRQQEELLAEENQRLREQLQLLEEQHRAALA
                     HSEEVTASQVAATKTLPNGRDALDGPAAEAEPEHSFDGLRRKVSAQRLQEAGILSAEE
                     LQRLAQGHTTVDELARREDVRHYLQGRSSIAGLLLKATNEKLSVYAALQRQLLSPGTA
                     LILLEAQAASGFLLDPVRNRRLTVNEAVKEGVVGPELHHKLLSAERAVTGYKDPYTGQ
                     QISLFQAMQKGLIVREHGIRLLEAQIATGGVIDPVHSHRVPVDVAYRRGYFDEEMNRV
                     LADPSDDTKGFFDPNTHENLTYLQLLERCVEDPETGLCLLPLTDKAAKGGELVYTDSE
                     ARDVFEKATVSAPFGKFQGKTVTIWEIINSEYFTAEQRRDLLRQFRTGRITVEKIIKI
                     IITVVEEQEQKGRLCFEGLRSLVPAAELLESRVIDRELYQQLQRGERSVRDVAEVDTV
                     RRALRGANVIAGVWLEEAGQKLSIYNALKKDLLPSDMAVALLEAQAGTGHIIDPATSA
                     RLTVDEAVRAGLVGPEFHEKLLSAEKAVTGYRDPYTGQSVSLFQALKKGLIPREQGLR
                     LLDAQLSTGGIVDPSKSHRVPLDVACARGCLDEETSRALSAPRADAKAYSDPSTGEPA
                     TYGELQQRCRPDQLTGLSLLPLSEKAARARQEELYSELQARETFEKTPVEVPVGGFKG
                     RTVTVWELISSEYFTAEQRQELLRQFRTGKVTVEKVIKILITIVEEVETLRQERLSFS
                     GLRAPVPASELLASGVLSRAQFEQLKDGKTTVKDLSELGSVRTLLQGSGCLAGIYLED
                     TKEKVSIYEAMRRGLLRATTAALLLEAQAATGFLVDPVRNQRLYVHEAVKAGVVGPEL
                     HEQLLSAEKAVTGYRDPYSGSTISLFQAMQKGLVLRQHGIRLLEAQIATGGIIDPVHS
                     HRVPVDVAYQRGYFSEEMNRVLADPSDDTKGFFDPNTHENLTYRQLLERCVEDPETGL
                     RLLPLKGAEKAEVVETTQVYTEEETRRAFEETQIDIPGGGSHGGSTMSLWEVMQSDLI
                     PEEQRAQLMADFQAGRVTKERMIIIIIEIIEKTEIIRQQGLASYDYVRRRLTAEDLFE
                     ARIISLETYNLLREGTRSLREALEAESAWCYLYGTGSVAGVYLPGSRQTLSIYQALKK
                     GLLSAEVARLLLEAQAATGFLLDPVKGERLTVDEAVRKGLVGPELHDRLLSAERAVTG
                     YRDPYTEQTISLFQAMKKELIPTEEALRLLDAQLATGGIVDPRLGFHLPLEVAYQRGY
                     LNKDTHDQLSEPSEVRSYVDPSTDERLSYTQLLRRCRRDDGTGQLLLPLSDARKLTFR
                     GLRKQITMEELVRSQVMDEATALQLREGLTSIEEVTKNLQKFLEGTSCIAGVFVDATK
                     ERLSVYQAMKKGIIRPGTAFELLEAQAATGYVIDPIKGLKLTVEEAVRMGIVGPEFKD
                     KLLSAERAVTGYKDPYSGKLISLFQAMKKGLILKDHGIRLLEAQIATGGIIDPEESHR
                     LPVEVAYKRGLFDEEMNEILTDPSDDTKGFFDPNTEENLTYLQLMERCITDPQTGLCL
                     LPLKEKKRERKTSSKSSVRKRRVVIVDPETGKEMSVYEAYRKGLIDHQTYLELSEQEC
                     EWEEITISSSDGVVKSMIIDRRSGRQYDIDDAIAKNLIDRSALDQYRAGTLSITEFAD
                     MLSGNAGGFRSRSSSVGSSSSYPISPAVSRTQLASWSDPTEETGPVAGILDTETLEKV
                     SITEAMHRNLVDNITGQRLLEAQACTGGIIDPSTGERFPVTDAVNKGLVDKIMVDRIN
                     LAQKAFCGFEDPRTKTKMSAAQALKKGWLYYEAGQRFLEVQYLTGGLIEPDTPGRVPL
                     DEALQRGTVDARTAQKLRDVGAYSKYLTCPKTKLKISYKDALDRSMVEEGTGLRLLEA
                     AAQSTKGYYSPYSVSGSGSTAGSRTGSRTGSRAGSRRGSFDATGSGFSMTFSSSSYSS
                     SGYGRRYASGSSASLGGPESAVA"
     exon            128..189
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            190..279
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            280..357
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            358..450
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            451..617
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            618..733
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            734..840
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            841..960
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            961..1056
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1057..1184
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1185..1278
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1279..1433
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1434..1752
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1753..1830
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1831..1992
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1993..2097
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2098..2193
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2194..2319
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2320..2472
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2473..2627
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2628..2754
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2755..2938
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2939..3096
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3097..3275
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3276..3414
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3415..3771
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3772..3855
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3856..3960
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3961..4059
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            4060..7440
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            7441..14683
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      14663..14668
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /note="hexamer: AATAAA"
     polyA_site      14683
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /note="major polyA site"
ORIGIN      
        1 agagtggccg ccgggtgctg gaggctccgc tgcaccccgc gccccgcgcc cgccggcatg
       61 gccggcccgc tgcccgacga gcaggacttc atccaggcct acgaggaggt gcgcgagaag
      121 tacaaagatg agcgggatcg tgtgcagaag aaaaccttca ccaagtgggt caacaagcac
      181 ctcatcaagg cccagaggca catcagtgac ctgtatgaag acctccgcga tggccacaac
      241 ctcatctccc tgctggaggt cctctcgggg gacagcctgc cccgggagaa ggggaggatg
      301 cgtttccaca agctgcagaa tgtccagatt gccctggact acctccggca ccgccaggtg
      361 aagctggtga acatcaggaa tgatgacatc gctgacggca accccaagct gacccttggc
      421 ctcatctgga caatcattct gcacttccag atctcagata tccaggtgag tgggcagtcg
      481 gaggacatga cggccaagga gaagctgctg ctgtggtcgc agcgaatggt ggaggggtac
      541 cagggcctgc gatgcgacaa cttcacctcc agctggagag acggccgcct cttcaatgcc
      601 atcatccacc ggcacaagcc cctgctcatc gacatgaaca aggtgtaccg gcagaccaac
      661 ctggagaacc tggaccaggc cttctctgtg gcggagcggg acctgggagt gacgcggctc
      721 ctggaccctg aggacgtgga tgtccctcag cccgacgaga agtccatcat cacctacgtc
      781 tcgtcgctgt atgacgccat gccccgcgtg ccggacgtgc aggatggggt gagggccaac
      841 gagctgcagc tgcgctggca ggagtaccgg gagctggtgc tgctgctgct tcagtggatg
      901 cgacaccaca cggccgcctt tgaggaacgc aggttcccct ccagcttcga ggagattgag
      961 atcctgtggt ctcagttcct gaagtttaag gagatggagc taccagccaa ggaggccgac
     1021 aagaacaggt ccaagggcat ctaccaatcc ctggagggag cggtgcaagc aggccagctc
     1081 aaggtgcccc ctggctacca cccgctggat gtggagaagg agtggggcaa gctgcacgtg
     1141 gccatcctgg agcgggagaa gcagctccgc agcgagtttg agaggctgga gtgtcttcag
     1201 cgcatcgtga ccaagctgca gatggaggcg gggctgtgtg aggagcagct gaaccaggcc
     1261 gacgccctgc tgcagtcgga tgtccggctg ctggctgcag gcaaagtgcc acagcgggcg
     1321 ggggaggtgg aacgggactt ggacaaggcg gatagcatga tccggctgct cttcaacgac
     1381 gtgcagaccc tcaaggatgg acggcacccg cagggcgagc agatgtaccg cagggtgtac
     1441 cgtctgcacg agcgcctggt agccatccgc accgagtaca acctacggct gaaggcaggc
     1501 gtggcggccc ctgcaaccca ggtggcccag gtgactctgc agagtgtgca gaggcgcccc
     1561 gagctggagg actccactct gcgctacctg caggacctgc tggcctgggt ggaggagaac
     1621 cagcaccgtg tggatggcgc tgagtggggt gtggacctgc ccagcgtgga ggcgcagctg
     1681 ggcagccacc gaggcctgca ccagtccatc gaagaattcc gggccaagat cgagcgggca
     1741 cggagtgacg agggccagct ctcccccgcc acccggggtg cctaccgtga ctgcctgggt
     1801 cggctggacc tgcagtacgc caagctgctg aactcctcca aggcccgcct caggtccctg
     1861 gagagcttgc acagctttgt ggcagccgcc actaaggagc taatgtggct gaatgagaag
     1921 gaggaggagg aggtgggctt cgactggagc gaccgcaaca ccaacatgac cgccaagaag
     1981 gagagctact cggcgctgat gcgggagctg gagctgaagg agaagaagat caaggagctc
     2041 caaaatgctg gggaccggct gctgcgggag gaccacccgg cccggcccac ggtggagtcc
     2101 ttccaggcgg ccctgcagac gcagtggagc tggatgctac agctgtgctg ctgtatcgag
     2161 gcacacctga aggagaacgc tgcctacttt cagttcttct cagatgtgcg ggaggccgag
     2221 gggcagttgc agaagctgca ggaggcactg cgtaggaaat acagttgtga tcgctccgcc
     2281 accgtcaccc ggctggagga cctgctgcag gatgcccagg acgagaagga acagctgaac
     2341 gagtacaagg gccacctctc aggcctggcc aagcgggcca aggccgtcgt gcagctgaag
     2401 ccccgccacc cagcccaccc catgcggggc cgcctgcccc tgctggccgt gtgcgactat
     2461 aagcaggtgg aggtgactgt gcacaagggt gacgagtgcc agctggtggg ccctgcacag
     2521 ccgtcccact ggaaggtgct cagcagctcc ggcagcgagg ccgccgtgcc ctccgtgtgc
     2581 ttcctggtgc ccccgcccaa ccaggaggcc caggaggccg tcaccaggct ggaggcccag
     2641 caccaggccc tggtcacgct gtggcaccag ttgcacgtgg acatgaagag ccttctggcc
     2701 tggcagagcc ttcgccgcga cgtgcagctc atccgctcct ggtccctggc cacgttccgc
     2761 accctgaagc cagaggagca gcgccaagcc ctgcacagcc tggagctgca ctaccaggcc
     2821 ttcctgcggg acagccagga cgcgggcggc ttcggacccg aggaccggct gatggctgag
     2881 cgcgagtacg gctcctgcag ccaccactac cagcagctgc tgcagagcct ggaacagggt
     2941 gcacaggaag agtctcgctg ccagcgctgc atctccgagc tcaaagacat ccggctgcag
     3001 ctggaggcct gtgagacgcg caccgtgcac cgcctgcggc tgccgctgga caaagagccg
     3061 gcacgggagt gtgcccagcg catcgccgag cagcagaagg cacaggcaga ggtggagggg
     3121 ctgggcaagg gggtcgcccg gctctctgcc gaggccgaga aggtcttggc cctaccagag
     3181 ccatcgcctg cggcccccac gctgcgctcg gagctggagc tgacgctggg caagctggag
     3241 caggtccgca gcctgtctgc catctacctg gagaagctca agaccatcag cctggtgatc
     3301 cgcggcacgc agggggccga ggaggtgctc agggcccacg aggagcagct caaggaggcc
     3361 caggccgtgc cggccaccct cccggagctc gaggccacca aggcctctct gaagaagctg
     3421 cgggcccagg ccgaggcaca gcagcccacg ttcgacgccc tgcgggatga gctgcggggg
     3481 gcacaggagg tgggggagcg actgcagcag cggcacgggg agcgggacgt ggaggtggag
     3541 cgctggcggg agcgggtcgc ccagttgctt gagcgctggc aggctgtgct ggcccagacc
     3601 gacgtgcggc agcgcgagct cgagcaactg ggccgccagc tgcgttacta ccgcgagagt
     3661 gcagacccct tgggcgcctg gctgcaggac gccaggcggc ggcaggagca gatccaggcc
     3721 atgccgctgg ccgacagcca ggctgtgcgg gagcagctgc ggcaggagca ggccctgctg
     3781 gaggagatcg agcgccacgg cgagaaggtc gaggagtgcc agaggtttgc gaaacagtac
     3841 atcaacgcca tcaaggacta tgaactccag ctggtgacgt acaaggcgca gcttgagccg
     3901 gtggcctccc cggccaagaa gcccaaggtc cagtcgggat cagagagtgt catccaggag
     3961 tacgtggacc tgcgtacgca ctacagcgag ctgaccacac tgacgagcca gtacatcaag
     4021 ttcatcagcg agactctgcg gcgcatggag gaggaggaga ggctggctga gcagcagcgg
     4081 gcagaggagc gcgagcggct ggccgaggtg gaggccgcgc tggagaagca gcggcagctg
     4141 gccgaggcgc acgcccaggc aaaggcacag gcggagcggg aggcgaagga gctgcagcag
     4201 cgcatgcagg aggaggtggt gcggcgggag gaggcggcgg tggacgcgca gcagcagaag
     4261 cgcagcattc aggaggagct gcagcagctg cggcagagct cggaggcgga gatccaggcc
     4321 aaggcccggc aggcagaggc ggctgagcgc agccggctgc gcatcgagga ggagatccgc
     4381 gtggtgcgcc tgcagttgga ggccaccgag cgccagcgtg gcggggctga gggggagctg
     4441 caggcactgc gtgcacgggc ggaggaggct gaggcacaaa agcgacaggc gcaggaggag
     4501 gccgagcgct tgcggaggca ggtgcaggac gagagccagc gtaagcggca ggcggaggtg
     4561 gagctggcct cgcgcgtgaa ggccgaggcc gaggcggcgc gcgagaagca gcgggccctg
     4621 caggccctgg aggagctgcg gctgcaggcg gaggaggcgg agcggcgcct gcggcaggcc
     4681 gaggtggagc gagcgcggca ggtacaggtg gccctggaga cggcgcagcg cagtgcagag
     4741 gcggagctgc agagcaaacg cgcctccttc gccgagaaga cggcacagct ggagcgctcc
     4801 ctgcaggagg aacacgtggc tgtggcacag ctgcgggagg aggctgagcg gcgggcacag
     4861 cagcaggccg aggccgagcg ggcgcgcgag gaggcagagc gggagctgga gcgctggcag
     4921 ctcaaggcca acgaggcgct acggctgcgg ctgcaggcgg aggaggtggc gcagcagaag
     4981 agcctggcgc aggccgaggc tgagaagcag aaggaggagg cggagcgcga ggcgcggcgg
     5041 cgcggcaagg cggaggagca ggccgtccgg cagcgggagc tggctgaaca agagctggag
     5101 aagcagcggc agctggcgga aggcaccgcg cagcagcgcc tggccgcgga gcaggagttg
     5161 atccggctgc gggccgagac ggagcagggg gagcagcagc ggcagctgct ggaggaggag
     5221 ctggcccggc tgcagcgtga ggcggctgca gccacgcaga aacggcagga gctggaagcc
     5281 gagctggcca aggtgcgggc cgagatggag gtgctgctgg ccagcaaggc gagggctgag
     5341 gaggagtcgc gctccaccag cgagaagtcc aagcagaggc tggaggccga ggccggccgg
     5401 ttccgcgagc tggccgagga ggccgcccgc ctgcgtgccc tggcggaaga ggccaagcgg
     5461 cagcggcagc tggccgagga agacgcggcg cggcagcggg ccgaggcgga gcgggtgctt
     5521 gcggagaagc tggccgccat cggcgaggcc acgcggctca agacggaggc ggagatcgcg
     5581 ctcaaggaga aggaggcgga gaacgagcgc ctgcggcggc tggcggagga cgaggccttc
     5641 cagcggcggc ggctggagga gcaggccgcg caacacaagg ctgacatcga ggagcgcctg
     5701 gcccagctgc gcaaggcatc ggacagcgag ctggagcggc agaaggggct ggtggaggac
     5761 acgctgaggc agcggcggca ggtggaggag gagatcctgg cgctgaaggc gagcttcgag
     5821 aaggcggccg ctggcaaggc ggagctggag ctggagctgg gacgcatccg cagcaacgcg
     5881 gaggacacgc tgcgcagcaa ggagcaggcc gagctggagg ctgcgaggca gcggcagctg
     5941 gcggcggagg aggagcggcg gcgccgtgag gctgaggagc gcgtgcagaa gagcctggcg
     6001 gccgaggagg aggccgcacg gcagcggaag gcggcgctgg aggaagtcga gcggctgaaa
     6061 gccaaggtgg aggaggcgcg gcgcctgcgg gagcgagcgg agcaggagtc ggcgcggcag
     6121 ctgcagctgg cccaggaggc cgcccagaag cggctgcagg cggaagagaa ggcacacgcc
     6181 ttcgcggtgc agcagaagga gcaggagcta cagcagacgc tgcagcagga gcagagcgtg
     6241 ctggaccagc tgcgcggcga ggcggaggcg gcccggcggg cggctgagga ggcggaggag
     6301 gcccgggtgc aggcggagcg tgaggcggcg cagtcccggc ggcaggtgga agaggccgag
     6361 cggctgaagc agtcggcaga ggagcaggca caggcccggg ctcaggcaca ggcggctgca
     6421 gagaagctgc gcaaggaggc cgagcaagag gcggcgcggc gggcacaggc ggagcaggcg
     6481 gccctgcggc agaagcaggc agctgacgcg gagatggaga agcataagaa attcgccgag
     6541 cagacgctgc ggcagaaggc gcaggtggag caggagctga caacactgcg gctgcagctg
     6601 gaggagaccg accaccagaa gaacctgctg gacgaggagc tgcagcggct gaaggcggag
     6661 gccacggagg ccgcacgcca gcgcagccag gtggaggagg agctcttctc ggtgcgcgtg
     6721 cagatggagg agctgagcaa gctcaaggca cgcatcgagg ctgagaaccg cgcactcatc
     6781 ttgcgtgaca aggacaatac gcagcgcttc ctgcaggagg aggctgagaa gatgaagcag
     6841 gtggcggagg aggccgcgcg gctgagtgtg gcggcccaag aggctgcgcg actgcggcag
     6901 ctggcagagg aggacctggc acagcagcgg gccttggcag agaagatgct caaggagaag
     6961 atgcaggcgg tgcaggaggc cacgcgactc aaggctgagg cggaactgct gcagcagcag
     7021 aaggagcttg cgcaggagca ggcgcggcgg ctgcaggagg acaaggagca gatggcgcag
     7081 cagctggcgg aggagacgca gggcttccag cggacgctgg aggccgagcg gcagcggcag
     7141 ctggagatga gcgctgaggc tgagcgcctc aagctgcgtg tggccgagat gagccgagcc
     7201 caggcccgcg ctgaggagga cgcccagcgc ttccggaagc aggcggagga gatcggtgag
     7261 aagctgcacc gcacggagct cgccacccag gagaaggtga ccctggtgca gacactggag
     7321 atccagcgac agcagagtga ccatgatgcc gagcgcctgc gggaggccat cgctgagctg
     7381 gagcgtgaga aggagaagct ccaacaggag gccaaactgc tgcagctcaa gtctgaggag
     7441 atgcagacgg tgcagcagga gcagctgctg caggagacgc aggccctgca gcaaagcttc
     7501 ctctctgaaa aggacagcct gctacagcgg gagcgcttca tcgagcagga gaaggccaag
     7561 ctggagcagc tcttccagga cgaggtggcc aaggcacagc agctgcgtga ggagcagcag
     7621 cggcagcagc agcagatgga gcaggaacgg cagcggctgg tggccagcat ggaggaggcg
     7681 cggcggcggc agcatgaggc cgaggagggc gtgcggcgca agcaggagga gctgcagcag
     7741 ctggagcagc agcggcggca gcaggaggag ctgctggctg aggagaacca gaggctgcgt
     7801 gagcagctgc agctcctgga ggagcagcac cgggccgcgc tggcgcactc agaggaggtc
     7861 actgcctcgc aggtggctgc cacaaagacc ctgcccaatg gccgggatgc acttgatggc
     7921 cccgcggcag aggcagagcc ggagcacagc ttcgatggcc tgcggcggaa ggtgtcagct
     7981 cagaggctgc aggaggccgg catcctgagt gcggaggagc tgcagcggtt ggcgcagggc
     8041 cacaccacgg tggacgagct cgcacggcgg gaagacgtgc gccactacct gcagggccgc
     8101 agcagtatcg cagggctgtt gctgaaggcc accaatgaga agctgagtgt ttacgccgcc
     8161 ctgcagaggc agctgctgag tcccggcacg gccctcatcc tgctggaggc gcaggcggcc
     8221 tcaggcttcc tgctggaccc tgtgcggaac cggcggctga ccgtcaacga ggctgtgaag
     8281 gagggtgtgg tgggccccga gctgcaccac aagctgctgt cggccgagcg cgccgtcact
     8341 ggctacaagg acccctacac tggccagcag atctctctct tccaagccat gcagaagggc
     8401 ctcatcgtcc gggagcacgg catccgcctg ctggaggccc agatcgccac gggcggcgtt
     8461 atcgaccccg tgcacagcca ccgcgtgccc gtggacgtgg cctaccggcg cggctacttc
     8521 gacgaggaga tgaaccgcgt cctggcggac cccagcgacg acaccaaggg cttctttgac
     8581 cccaacacgc acgagaacct cacgtacctg cagctactgg agcgctgcgt ggaggacccc
     8641 gagacgggcc tgtgccttct gccactcacg gataaggctg ccaagggcgg ggagctggtc
     8701 tacactgact ccgaggcccg ggacgtcttt gagaaggcca ccgtgtctgc gccgttcggc
     8761 aagttccagg gcaagacggt gaccatttgg gagatcatca actcggaata cttcacggca
     8821 gagcagcggc gggacctgct gcggcagttc cgcacgggcc ggatcacagt ggagaagatc
     8881 atcaagatca tcatcacggt ggtggaggag caggagcaga agggccggct ttgctttgag
     8941 ggcctgcgca gcctggtgcc agccgccgag ctgctggaga gcagggtcat cgaccgcgag
     9001 ctctaccagc agctgcagcg aggtgagcgc tctgtgcgag acgtagccga ggtggacact
     9061 gtgcggcggg ctctccgggg tgccaacgtc atcgcgggtg tatggctgga ggaggcgggg
     9121 cagaagctga gtatctacaa tgccctgaag aaagacctgc tgccatccga catggccgtg
     9181 gccctgttgg aagcccaggc cggcaccggg cacatcatcg accccgccac cagcgcccgg
     9241 ctgaccgtgg acgaggcagt gcgtgctggc ctggtgggcc ccgagtttca tgagaagctg
     9301 ctatcagccg agaaggctgt gacagggtac agggacccct acacagggca gagcgtctcc
     9361 ctgttccagg ccctgaagaa gggcctcatt ccccgggagc agggcctgcg cctgttggac
     9421 gcccagctgt ccacgggcgg catcgtggac cccagcaaga gccaccgcgt gcccctggat
     9481 gtcgcctgcg cccgaggctg cctggatgag gagaccagca gggccctgtc ggcaccaagg
     9541 gccgacgcca aggcctacag tgaccccagc acaggggagc cggccaccta cggcgagctc
     9601 cagcagcggt gccggcccga ccagctgacc gggctgagcc tgctgccgct ctcagaaaag
     9661 gctgctcggg cccggcagga ggagctctac tcagagctgc aggcccgtga gacctttgaa
     9721 aagaccccgg ttgaggtccc cgtgggtggc ttcaagggca ggacggtgac ggtgtgggag
     9781 ctcatcagct ctgagtactt cactgcggag cagcggcagg agctgttgcg tcagttccgc
     9841 acgggcaagg tcaccgtgga gaaggtcatc aagattctca ttaccatcgt ggaggaggtg
     9901 gagaccctgc ggcaggagag gctgtccttc agcggcctcc gtgcccctgt gccagccagc
     9961 gagctcctgg cttccggggt cctcagcaga gcccagtttg agcagctcaa ggacggcaag
    10021 acgacggtca aggacctttc ggagctgggc tccgtgcgga cgctgctgca gggcagtggc
    10081 tgcctcgccg gcatctacct ggaggacacc aaggagaagg tgtccatcta cgaggccatg
    10141 cgccggggcc tgctgagagc cacaacggct gcgctcctgc tggaggcgca ggcggccact
    10201 ggcttcctgg tggaccccgt gcggaaccag cgcctgtatg tccacgaggc cgtgaaggcg
    10261 ggcgtggtgg gccccgagct tcacgagcag ctgctgtctg ccgagaaggc cgtcaccggc
    10321 tacagagacc cctactcggg cagcaccatc tccctcttcc aggccatgca gaagggcctg
    10381 gttctccggc agcacggcat ccgcctgctg gaggcccaga tcgccacggg cggcatcatc
    10441 gaccccgtgc acagccaccg cgtgcctgtg gacgtggcct accagcgcgg ctacttcagt
    10501 gaggagatga accgcgtcct ggcggacccc agcgacgaca ccaagggctt ctttgacccc
    10561 aacacgcatg agaacctcac gtacaggcag ctgctggagc ggtgcgtgga ggaccccgag
    10621 acgggcttgc gccttctgcc actgaaaggg gcggagaagg ctgaggtggt ggagaccacg
    10681 caggtgtaca ctgaggagga gacaagaagg gcatttgaag agacacagat cgacattccc
    10741 ggcggcggca gccacggcgg ctccaccatg tccctgtggg aggtgatgca gtcggacctg
    10801 atccccgagg agcagcgggc ccagctgatg gctgacttcc aggccggccg ggtgaccaag
    10861 gaacgcatga tcatcatcat catcgagatc attgagaaga cagagatcat ccgccagcag
    10921 ggtctggcct cctacgacta cgtgcgccgc cgcctcacgg ctgaggacct gttcgaggct
    10981 cggatcatct ctctcgagac ctacaacctg ctccgggagg gcaccaggag cctccgtgag
    11041 gctctcgagg cggagtccgc ctggtgctac ctctatggca cgggctccgt ggctggtgtc
    11101 tacctgcccg gttccaggca gacactgagc atctaccagg ctctcaagaa agggctgctg
    11161 agtgccgagg tggcccgcct gctgctggag gcacaggcag ccacaggctt cctgctggac
    11221 ccggtgaagg gggagcggct gactgtggat gaggctgtgc ggaagggcct cgtggggccc
    11281 gagctgcacg accgcctgct ctcggctgag cgggcggtca ccggctaccg tgacccctac
    11341 accgagcaga ccatctcgct cttccaggcc atgaagaagg agctgatccc tactgaggag
    11401 gccctgcggc tgctggatgc ccagctggcc accggcggca tcgtggaccc ccgcctgggc
    11461 ttccaccttc ccctggaggt ggcttaccag cgtggctacc tcaacaagga cacgcacgac
    11521 cagctgtcag agcccagcga ggtgcgcagc tacgtggacc cgtccaccga cgagcgcctc
    11581 agctacacgc agctgctcag gcggtgccgt cgtgacgacg gcaccggcca gctgctcctg
    11641 ccactgtcgg acgcccgcaa gctgaccttc cgtggcctgc ggaagcagat caccatggag
    11701 gagctggtgc gctcgcaggt catggacgag gccacggcgc tgcagctgcg ggagggcctg
    11761 acctccatcg aggaggtcac caagaacttg cagaagttcc tggaaggcac cagctgcatc
    11821 gctggtgtct tcgtggacgc caccaaggaa cggctctcgg tgtaccaggc catgaagaag
    11881 ggcatcatcc gccccggcac agcctttgag ctcctggagg cgcaggcggc caccggttac
    11941 gtcatcgacc ccatcaaggg actgaagctg acggtggagg aggctgtgcg tatgggcatt
    12001 gtgggccccg agttcaagga caagctgctg tcggccgagc gcgccgtcac tgggtacaag
    12061 gacccctact ctgggaagct catctccctc ttccaggcca tgaagaaggg cctgatcctg
    12121 aaggaccatg gcatccgcct gctggaggcc cagatcgcca cgggcggcat catcgaccct
    12181 gaggagagcc accggctgcc cgtggaggtg gcctacaagc gcggcctctt cgatgaggag
    12241 atgaacgaga tcctgaccga cccctcggac gacaccaagg gcttctttga ccctaacacg
    12301 gaggagaacc tcacctacct gcagctgatg gagcgttgta tcactgaccc ccagacgggc
    12361 ctgtgtctct tgccgctgaa ggagaagaag cgggagcgga agacgtcctc caagtcctcc
    12421 gtgcgcaagc gccgagtggt catcgtggac cccgagacgg gcaaggagat gtcagtgtac
    12481 gaggcctacc gcaagggcct gattgaccac cagacgtacc tggagctgtc cgagcaggag
    12541 tgcgagtggg aggagatcac catctcctcc tcggacggcg tggtcaagtc catgatcatc
    12601 gaccgccgct ccgggcgcca gtacgacatc gatgatgcca tcgccaagaa cctcatcgac
    12661 cgctcggcac tggaccagta ccgcgccggc acgctctcca tcaccgagtt cgccgacatg
    12721 ctctcgggca acgccggtgg tttccgctcc cgttcctcct cggtgggatc ctcctcctcc
    12781 taccccatca gccccgccgt ctccaggacc cagctggcct cctggtcaga ccccactgag
    12841 gagacgggcc ccgtggctgg catcctggac acggagacgc tggagaaggt gtccatcacc
    12901 gaggccatgc accggaacct ggtggataac atcacggggc agcggctgct ggaggcgcag
    12961 gcctgcaccg ggggcatcat cgaccccagc accggtgagc gcttccctgt caccgacgcc
    13021 gtcaacaagg gcctggtgga caagatcatg gtggaccgca tcaacctggc ccagaaggcc
    13081 ttctgcggct tcgaggaccc acgcaccaag accaagatgt cggccgccca ggccctgaag
    13141 aagggctggc tctactacga ggccggccag cgcttcctgg aggtgcagta cctgaccggc
    13201 ggcttgatcg agcccgacac gccgggccgc gtgcccctgg acgaggccct gcagcgcggc
    13261 acggtggacg cccgcaccgc acagaagctg cgtgacgtgg gcgcctactc caagtacctc
    13321 acctgcccta agaccaagct caagatctcc tataaggacg cgctggaccg cagcatggtg
    13381 gaggagggca cggggctgcg gctgctggag gctgccgcgc agtccaccaa gggctactac
    13441 agcccctaca gcgtcagcgg ctccggctct accgctggct cccgcaccgg ctcgcgcacc
    13501 ggctcccggg ccggctcccg ccgcggcagc tttgacgcca ccggctccgg cttctccatg
    13561 accttctctt catcctccta ctcctcctcg ggctacggcc gccgctacgc ctcggggtcc
    13621 tcggcctccc tggggggccc tgagtctgcc gtggcctgag gctgcctgcg cccaccccgc
    13681 tctgcatgcg gcccagcccg gctcccaccg aggcgcgggg gccgttttca acgcttaaag
    13741 gtgtcttcct cccaagtggt gcctaaagtt taaccaaaaa gaccagacta atatattaat
    13801 atatatctgc tgtccagaca gcctgtatct tgggggacag ggctggccca gccctgctgg
    13861 ccgcctcacc ccctcgggtc tcctcactcc cttctacctg ccactcacac agccaggtgc
    13921 cttggagggt cccaagctgg gccccagccc accctcctgt cttcccaggg tagcccgcct
    13981 gccagtccta gctgcacagg gcagctgggc ccaaccctgt ctgtagaggg ccctggtgtt
    14041 tctagcactg gcctgcacgg tgggccttgc tggggacggg gggccccagt cagcctctct
    14101 cccagtctac ccagagaagc cccttcccca tgggaagacg aggccctcgg gcccagcccc
    14161 cacagtgctg tctgatctgt gctttccagc tcacccccca cactcactcc tgagacccct
    14221 ggcctccggc gtcagcctcc agcctctgtt cccctagtaa gtgccttcca tgtcggcctc
    14281 taaccccagg ccccgaggac ccagacccag tggggaggcg gacgttccag ccggcatggc
    14341 tgggaactgc agacctgtcc tcctggtggg tccaggggcc cctccagctt gtggagcccc
    14401 acactggggt gccgcctgcc cgtctctctc ccatggagcc ccagccccct ttgggcccag
    14461 ggacaccagc caggctctgt gctgaccctc ctgttgcacc cagccctggt ctcagcagcg
    14521 accacccctg cctccaccct ctgagctttg catgttccac taaccccggg cgggtggcag
    14581 gtggaggtgt caggctgctg gcgcctctgc aagggcagaa cactaacctg accgtgggcg
    14641 gggccttgcg gtatccgccc ccaataaaag caattccaac ctt
//
