LOCUS       NM_000445              14798 bp    mRNA    linear   PRI 11-OCT-2024
DEFINITION  Homo sapiens plectin (PLEC), transcript variant 1, mRNA.
ACCESSION   NM_000445
VERSION     NM_000445.5
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 14798)
  AUTHORS   Rezniczek,G.A., Walko,G. and Wiche,G.
  TITLE     Plectin gene defects lead to various forms of epidermolysis bullosa
            simplex
  JOURNAL   Dermatol Clin 28 (1), 33-41 (2010)
   PUBMED   19945614
  REMARK    Review article
            Erratum:[Dermatol Clin. 2010 Apr;28(2):439-41]
REFERENCE   2  (bases 1 to 14798)
  AUTHORS   Winter,L., Abrahamsberg,C. and Wiche,G.
  TITLE     Plectin isoform 1b mediates mitochondrion-intermediate filament
            network linkage and controls organelle shape
  JOURNAL   J Cell Biol 181 (6), 903-911 (2008)
   PUBMED   18541706
REFERENCE   3  (bases 1 to 14798)
  AUTHORS   Sonnenberg,A. and Liem,R.K.
  TITLE     Plakins in development and disease
  JOURNAL   Exp Cell Res 313 (10), 2189-2203 (2007)
   PUBMED   17499243
  REMARK    Review article
REFERENCE   4  (bases 1 to 14798)
  AUTHORS   Rezniczek,G.A., Konieczny,P., Nikolic,B., Reipert,S., Schneller,D.,
            Abrahamsberg,C., Davies,K.E., Winder,S.J. and Wiche,G.
  TITLE     Plectin 1f scaffolding at the sarcolemma of dystrophic (mdx) muscle
            fibers through multiple interactions with beta-dystroglycan
  JOURNAL   J Cell Biol 176 (7), 965-977 (2007)
   PUBMED   17389230
REFERENCE   5  (bases 1 to 14798)
  AUTHORS   Pfendner,E., Rouan,F. and Uitto,J.
  TITLE     Progress in epidermolysis bullosa: the phenotypic spectrum of
            plectin mutations
  JOURNAL   Exp Dermatol 14 (4), 241-249 (2005)
   PUBMED   15810881
  REMARK    GeneRIF: discussion of phenotypic spectrum of plectin mutations
            [review]
            Review article
REFERENCE   6  (bases 1 to 14798)
  AUTHORS   Zhang,T., Haws,P. and Wu,Q.
  TITLE     Multiple variable first exons: a mechanism for cell- and
            tissue-specific gene regulation
  JOURNAL   Genome Res 14 (1), 79-89 (2004)
   PUBMED   14672974
REFERENCE   7  (bases 1 to 14798)
  AUTHORS   Rezniczek,G.A., Abrahamsberg,C., Fuchs,P., Spazierer,D. and
            Wiche,G.
  TITLE     Plectin 5'-transcript diversity: short alternative sequences
            determine stability of gene products, initiation of translation and
            subcellular localization of isoforms
  JOURNAL   Hum Mol Genet 12 (23), 3181-3194 (2003)
   PUBMED   14559777
REFERENCE   8  (bases 1 to 14798)
  AUTHORS   Andra,K., Kornacker,I., Jorgl,A., Zorer,M., Spazierer,D., Fuchs,P.,
            Fischer,I. and Wiche,G.
  TITLE     Plectin-isoform-specific rescue of hemidesmosomal defects in
            plectin (-/-) keratinocytes
  JOURNAL   J Invest Dermatol 120 (2), 189-197 (2003)
   PUBMED   12542521
REFERENCE   9  (bases 1 to 14798)
  AUTHORS   Leung,C.L., Green,K.J. and Liem,R.K.
  TITLE     Plakins: a family of versatile cytolinker proteins
  JOURNAL   Trends Cell Biol 12 (1), 37-45 (2002)
   PUBMED   11854008
  REMARK    Review article
REFERENCE   10 (bases 1 to 14798)
  AUTHORS   Brown,M.J., Hallam,J.A., Liu,Y., Yamada,K.M. and Shaw,S.
  TITLE     Cutting edge: integration of human T lymphocyte cytoskeleton by the
            cytolinker plectin
  JOURNAL   J Immunol 167 (2), 641-645 (2001)
   PUBMED   11441066
  REMARK    GeneRIF: During polarization of peripheral blood T lymphocytes,
            plectin redistributes to the uropod associated with vimentin and
            fodrin. This vimentin-plectin-fodrin complex provides a continuous
            linkage from the nucleus (lamin B) to the cortical cytoskeleton.
REFERENCE   11 (bases 1 to 14798)
  AUTHORS   Schroder,R., Furst,D.O., Klasen,C., Reimann,J., Herrmann,H. and van
            der Ven,P.F.
  TITLE     Association of plectin with Z-discs is a prerequisite for the
            formation of the intermyofibrillar desmin cytoskeleton
  JOURNAL   Lab Invest 80 (4), 455-464 (2000)
   PUBMED   10780662
REFERENCE   12 (bases 1 to 14798)
  AUTHORS   Fuchs,P., Zorer,M., Rezniczek,G.A., Spazierer,D., Oehler,S.,
            Castanon,M.J., Hauptmann,R. and Wiche,G.
  TITLE     Unusual 5' transcript complexity of plectin isoforms: novel
            tissue-specific exons modulate actin binding activity
  JOURNAL   Hum Mol Genet 8 (13), 2461-2472 (1999)
   PUBMED   10556294
REFERENCE   13 (bases 1 to 14798)
  AUTHORS   Wiche,G.
  TITLE     Role of plectin in cytoskeleton organization and dynamics
  JOURNAL   J Cell Sci 111 (Pt 17), 2477-2486 (1998)
   PUBMED   9701547
  REMARK    Review article
REFERENCE   14 (bases 1 to 14798)
  AUTHORS   Elliott,C.E., Becker,B., Oehler,S., Castanon,M.J., Hauptmann,R. and
            Wiche,G.
  TITLE     Plectin transcript diversity: identification and tissue
            distribution of variants with distinct first coding exons and
            rodless isoforms
  JOURNAL   Genomics 42 (1), 115-125 (1997)
   PUBMED   9177781
REFERENCE   15 (bases 1 to 14798)
  AUTHORS   Chavanas,S., Pulkkinen,L., Gache,Y., Smith,F.J., McLean,W.H.,
            Uitto,J., Ortonne,J.P. and Meneguzzi,G.
  TITLE     A homozygous nonsense mutation in the PLEC1 gene in patients with
            epidermolysis bullosa simplex with muscular dystrophy
  JOURNAL   J Clin Invest 98 (10), 2196-2200 (1996)
   PUBMED   8941634
REFERENCE   16 (bases 1 to 14798)
  AUTHORS   Pulkkinen,L., Smith,F.J., Shimizu,H., Murata,S., Yaoita,H.,
            Hachisuka,H., Nishikawa,T., McLean,W.H. and Uitto,J.
  TITLE     Homozygous deletion mutations in the plectin gene (PLEC1) in
            patients with epidermolysis bullosa simplex associated with
            late-onset muscular dystrophy
  JOURNAL   Hum Mol Genet 5 (10), 1539-1546 (1996)
   PUBMED   8894687
REFERENCE   17 (bases 1 to 14798)
  AUTHORS   Smith,F.J., Eady,R.A., Leigh,I.M., McMillan,J.R., Rugg,E.L.,
            Kelsell,D.P., Bryant,S.P., Spurr,N.K., Geddes,J.F., Kirtschig,G.,
            Milana,G., de Bono,A.G., Owaribe,K., Wiche,G., Pulkkinen,L.,
            Uitto,J., McLean,W.H. and Lane,E.B.
  TITLE     Plectin deficiency results in muscular dystrophy with epidermolysis
            bullosa
  JOURNAL   Nat Genet 13 (4), 450-457 (1996)
   PUBMED   8696340
REFERENCE   18 (bases 1 to 14798)
  AUTHORS   McLean,W.H., Pulkkinen,L., Smith,F.J., Rugg,E.L., Lane,E.B.,
            Bullrich,F., Burgeson,R.E., Amano,S., Hudson,D.L., Owaribe,K.,
            McGrath,J.A., McMillan,J.R., Eady,R.A., Leigh,I.M., Christiano,A.M.
            and Uitto,J.
  TITLE     Loss of plectin causes epidermolysis bullosa with muscular
            dystrophy: cDNA cloning and genomic organization
  JOURNAL   Genes Dev 10 (14), 1724-1735 (1996)
   PUBMED   8698233
REFERENCE   19 (bases 1 to 14798)
  AUTHORS   Gache,Y., Chavanas,S., Lacour,J.P., Wiche,G., Owaribe,K.,
            Meneguzzi,G. and Ortonne,J.P.
  TITLE     Defective expression of plectin/HD1 in epidermolysis bullosa
            simplex with muscular dystrophy
  JOURNAL   J Clin Invest 97 (10), 2289-2298 (1996)
   PUBMED   8636409
REFERENCE   20 (bases 1 to 14798)
  AUTHORS   Liu,C.G., Maercker,C., Castanon,M.J., Hauptmann,R. and Wiche,G.
  TITLE     Human plectin: organization of the gene, sequence analysis, and
            chromosome localization (8q24)
  JOURNAL   Proc Natl Acad Sci U S A 93 (9), 4278-4283 (1996)
   PUBMED   8633055
REFERENCE   21 (bases 1 to 14798)
  AUTHORS   Pegoraro,E. and Hoffman,E.P.
  TITLE     Limb-Girdle Muscular Dystrophy Overview - RETIRED CHAPTER, FOR
            HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301582
REFERENCE   22 (bases 1 to 14798)
  AUTHORS   So,J.Y. and Teng,J.
  TITLE     Epidermolysis Bullosa Simplex
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301543
REFERENCE   23 (bases 1 to 14798)
  AUTHORS   Abicht,A., Muller,J.S. and Lochmuller,H.
  TITLE     Congenital Myasthenic Syndromes Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301347
REFERENCE   24 (bases 1 to 14798)
  AUTHORS   Lucky,A.W. and Gorell,E.
  TITLE     Epidermolysis Bullosa with Pyloric Atresia
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301336
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff in
            collaboration with Gunther Rezniczek, Gerhard Wiche. The reference
            sequence was derived from BP312608.1 and AY480044.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_000445.4.
            
            Summary: Plectin is a prominent member of an important family of
            structurally and in part functionally related proteins, termed
            plakins or cytolinkers, that are capable of interlinking different
            elements of the cytoskeleton. Plakins, with their multi-domain
            structure and enormous size, not only play crucial roles in
            maintaining cell and tissue integrity and orchestrating dynamic
            changes in cytoarchitecture and cell shape, but also serve as
            scaffolding platforms for the assembly, positioning, and regulation
            of signaling complexes (reviewed in PMID: 9701547, 11854008, and
            17499243). Plectin is expressed as several protein isoforms in a
            wide range of cell types and tissues from a single gene located on
            chromosome 8 in humans (PMID: 8633055, 8698233). Until 2010, this
            locus was named plectin 1 (symbol PLEC1 in human; Plec1 in mouse
            and rat) and the gene product had been referred to as
            'hemidesmosomal protein 1' or 'plectin 1, intermediate filament
            binding 500kDa'. These names were superseded by plectin. The
            plectin gene locus in mouse on chromosome 15 has been analyzed in
            detail (PMID: 10556294, 14559777), revealing a genomic exon-intron
            organization with well over 40 exons spanning over 62 kb and an
            unusual 5' transcript complexity of plectin isoforms. Eleven exons
            (1-1j) have been identified that alternatively splice directly into
            a common exon 2 which is the first exon to encode plectin's highly
            conserved actin binding domain (ABD). Three additional exons (-1,
            0a, and 0) splice into an alternative first coding exon (1c), and
            two additional exons (2alpha and 3alpha) are optionally spliced
            within the exons encoding the acting binding domain (exons 2-8).
            Analysis of the human locus has identified eight of the eleven
            alternative 5' exons found in mouse and rat (PMID: 14672974); exons
            1i, 1j and 1h have not been confirmed in human. Furthermore,
            isoforms lacking the central rod domain encoded by exon 31 have
            been detected in mouse (PMID:10556294), rat (PMID: 9177781), and
            human (PMID: 11441066, 10780662, 20052759). The short alternative
            amino-terminal sequences encoded by the different first exons
            direct the targeting of the various isoforms to distinct
            subcellular locations (PMID: 14559777). As the expression of
            specific plectin isoforms was found to be dependent on cell type
            (tissue) and stage of development (PMID: 10556294, 12542521,
            17389230) it appears that each cell type (tissue) contains a unique
            set (proportion and composition) of plectin isoforms, as if
            custom-made for specific requirements of the particular cells.
            Concordantly, individual isoforms were found to carry out distinct
            and specific functions (PMID: 14559777, 12542521, 18541706). In
            1996, a number of groups reported that patients suffering from
            epidermolysis bullosa simplex with muscular dystrophy (EBS-MD)
            lacked plectin expression in skin and muscle tissues due to defects
            in the plectin gene (PMID: 8698233, 8941634, 8636409, 8894687,
            8696340). Two other subtypes of plectin-related EBS have been
            described: EBS-pyloric atresia (PA) and EBS-Ogna. For reviews of
            plectin-related diseases see PMID: 15810881, 19945614. Mutations in
            the plectin gene related to human diseases should be named based on
            the position in NM_000445 (variant 1, isoform 1c), unless the
            mutation is located within one of the other alternative first
            exons, in which case the position in the respective Reference
            Sequence should be used. [provided by RefSeq, Aug 2011].
            
            Transcript Variant: This variant (1) contains the 5'-most coding
            exon (exon 1c) and encodes isoform 1c.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U53204.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: PMID: 18541706, 17389230
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-43                BP312608.1         1-43
            44-14798            AY480044.1         1-14755
FEATURES             Location/Qualifiers
     source          1..14798
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.3"
     gene            1..14798
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /note="plectin"
                     /db_xref="GeneID:5339"
                     /db_xref="HGNC:HGNC:9069"
                     /db_xref="MIM:601282"
     exon            1..43
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            44..242
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     CDS             50..13774
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /note="isoform 1c is encoded by transcript variant 1;
                     plectin 1, intermediate filament binding protein 500kDa;
                     hemidesmosomal protein 1"
                     /codon_start=1
                     /product="plectin isoform 1c"
                     /protein_id="NP_000436.2"
                     /db_xref="CCDS:CCDS43769.1"
                     /db_xref="GeneID:5339"
                     /db_xref="HGNC:HGNC:9069"
                     /db_xref="MIM:601282"
                     /translation="MSGEDAEVRAVSEDVSNGSSGSPSPGDTLPWNLGKTQRSRRSGG
                     GAGSNGSVLDPAERAVIRIADERDRVQKKTFTKWVNKHLIKAQRHISDLYEDLRDGHN
                     LISLLEVLSGDSLPREKGRMRFHKLQNVQIALDYLRHRQVKLVNIRNDDIADGNPKLT
                     LGLIWTIILHFQISDIQVSGQSEDMTAKEKLLLWSQRMVEGYQGLRCDNFTSSWRDGR
                     LFNAIIHRHKPLLIDMNKVYRQTNLENLDQAFSVAERDLGVTRLLDPEDVDVPQPDEK
                     SIITYVSSLYDAMPRVPDVQDGVRANELQLRWQEYRELVLLLLQWMRHHTAAFEERRF
                     PSSFEEIEILWSQFLKFKEMELPAKEADKNRSKGIYQSLEGAVQAGQLKVPPGYHPLD
                     VEKEWGKLHVAILEREKQLRSEFERLECLQRIVTKLQMEAGLCEEQLNQADALLQSDV
                     RLLAAGKVPQRAGEVERDLDKADSMIRLLFNDVQTLKDGRHPQGEQMYRRVYRLHERL
                     VAIRTEYNLRLKAGVAAPATQVAQVTLQSVQRRPELEDSTLRYLQDLLAWVEENQHRV
                     DGAEWGVDLPSVEAQLGSHRGLHQSIEEFRAKIERARSDEGQLSPATRGAYRDCLGRL
                     DLQYAKLLNSSKARLRSLESLHSFVAAATKELMWLNEKEEEEVGFDWSDRNTNMTAKK
                     ESYSALMRELELKEKKIKELQNAGDRLLREDHPARPTVESFQAALQTQWSWMLQLCCC
                     IEAHLKENAAYFQFFSDVREAEGQLQKLQEALRRKYSCDRSATVTRLEDLLQDAQDEK
                     EQLNEYKGHLSGLAKRAKAVVQLKPRHPAHPMRGRLPLLAVCDYKQVEVTVHKGDECQ
                     LVGPAQPSHWKVLSSSGSEAAVPSVCFLVPPPNQEAQEAVTRLEAQHQALVTLWHQLH
                     VDMKSLLAWQSLRRDVQLIRSWSLATFRTLKPEEQRQALHSLELHYQAFLRDSQDAGG
                     FGPEDRLMAEREYGSCSHHYQQLLQSLEQGAQEESRCQRCISELKDIRLQLEACETRT
                     VHRLRLPLDKEPARECAQRIAEQQKAQAEVEGLGKGVARLSAEAEKVLALPEPSPAAP
                     TLRSELELTLGKLEQVRSLSAIYLEKLKTISLVIRGTQGAEEVLRAHEEQLKEAQAVP
                     ATLPELEATKASLKKLRAQAEAQQPTFDALRDELRGAQEVGERLQQRHGERDVEVERW
                     RERVAQLLERWQAVLAQTDVRQRELEQLGRQLRYYRESADPLGAWLQDARRRQEQIQA
                     MPLADSQAVREQLRQEQALLEEIERHGEKVEECQRFAKQYINAIKDYELQLVTYKAQL
                     EPVASPAKKPKVQSGSESVIQEYVDLRTHYSELTTLTSQYIKFISETLRRMEEEERLA
                     EQQRAEERERLAEVEAALEKQRQLAEAHAQAKAQAEREAKELQQRMQEEVVRREEAAV
                     DAQQQKRSIQEELQQLRQSSEAEIQAKARQAEAAERSRLRIEEEIRVVRLQLEATERQ
                     RGGAEGELQALRARAEEAEAQKRQAQEEAERLRRQVQDESQRKRQAEVELASRVKAEA
                     EAAREKQRALQALEELRLQAEEAERRLRQAEVERARQVQVALETAQRSAEAELQSKRA
                     SFAEKTAQLERSLQEEHVAVAQLREEAERRAQQQAEAERAREEAERELERWQLKANEA
                     LRLRLQAEEVAQQKSLAQAEAEKQKEEAEREARRRGKAEEQAVRQRELAEQELEKQRQ
                     LAEGTAQQRLAAEQELIRLRAETEQGEQQRQLLEEELARLQREAAAATQKRQELEAEL
                     AKVRAEMEVLLASKARAEEESRSTSEKSKQRLEAEAGRFRELAEEAARLRALAEEAKR
                     QRQLAEEDAARQRAEAERVLAEKLAAIGEATRLKTEAEIALKEKEAENERLRRLAEDE
                     AFQRRRLEEQAAQHKADIEERLAQLRKASDSELERQKGLVEDTLRQRRQVEEEILALK
                     ASFEKAAAGKAELELELGRIRSNAEDTLRSKEQAELEAARQRQLAAEEERRRREAEER
                     VQKSLAAEEEAARQRKAALEEVERLKAKVEEARRLRERAEQESARQLQLAQEAAQKRL
                     QAEEKAHAFAVQQKEQELQQTLQQEQSVLDQLRGEAEAARRAAEEAEEARVQAEREAA
                     QSRRQVEEAERLKQSAEEQAQARAQAQAAAEKLRKEAEQEAARRAQAEQAALRQKQAA
                     DAEMEKHKKFAEQTLRQKAQVEQELTTLRLQLEETDHQKNLLDEELQRLKAEATEAAR
                     QRSQVEEELFSVRVQMEELSKLKARIEAENRALILRDKDNTQRFLQEEAEKMKQVAEE
                     AARLSVAAQEAARLRQLAEEDLAQQRALAEKMLKEKMQAVQEATRLKAEAELLQQQKE
                     LAQEQARRLQEDKEQMAQQLAEETQGFQRTLEAERQRQLEMSAEAERLKLRVAEMSRA
                     QARAEEDAQRFRKQAEEIGEKLHRTELATQEKVTLVQTLEIQRQQSDHDAERLREAIA
                     ELEREKEKLQQEAKLLQLKSEEMQTVQQEQLLQETQALQQSFLSEKDSLLQRERFIEQ
                     EKAKLEQLFQDEVAKAQQLREEQQRQQQQMEQERQRLVASMEEARRRQHEAEEGVRRK
                     QEELQQLEQQRRQQEELLAEENQRLREQLQLLEEQHRAALAHSEEVTASQVAATKTLP
                     NGRDALDGPAAEAEPEHSFDGLRRKVSAQRLQEAGILSAEELQRLAQGHTTVDELARR
                     EDVRHYLQGRSSIAGLLLKATNEKLSVYAALQRQLLSPGTALILLEAQAASGFLLDPV
                     RNRRLTVNEAVKEGVVGPELHHKLLSAERAVTGYKDPYTGQQISLFQAMQKGLIVREH
                     GIRLLEAQIATGGVIDPVHSHRVPVDVAYRRGYFDEEMNRVLADPSDDTKGFFDPNTH
                     ENLTYLQLLERCVEDPETGLCLLPLTDKAAKGGELVYTDSEARDVFEKATVSAPFGKF
                     QGKTVTIWEIINSEYFTAEQRRDLLRQFRTGRITVEKIIKIIITVVEEQEQKGRLCFE
                     GLRSLVPAAELLESRVIDRELYQQLQRGERSVRDVAEVDTVRRALRGANVIAGVWLEE
                     AGQKLSIYNALKKDLLPSDMAVALLEAQAGTGHIIDPATSARLTVDEAVRAGLVGPEF
                     HEKLLSAEKAVTGYRDPYTGQSVSLFQALKKGLIPREQGLRLLDAQLSTGGIVDPSKS
                     HRVPLDVACARGCLDEETSRALSAPRADAKAYSDPSTGEPATYGELQQRCRPDQLTGL
                     SLLPLSEKAARARQEELYSELQARETFEKTPVEVPVGGFKGRTVTVWELISSEYFTAE
                     QRQELLRQFRTGKVTVEKVIKILITIVEEVETLRQERLSFSGLRAPVPASELLASGVL
                     SRAQFEQLKDGKTTVKDLSELGSVRTLLQGSGCLAGIYLEDTKEKVSIYEAMRRGLLR
                     ATTAALLLEAQAATGFLVDPVRNQRLYVHEAVKAGVVGPELHEQLLSAEKAVTGYRDP
                     YSGSTISLFQAMQKGLVLRQHGIRLLEAQIATGGIIDPVHSHRVPVDVAYQRGYFSEE
                     MNRVLADPSDDTKGFFDPNTHENLTYRQLLERCVEDPETGLRLLPLKGAEKAEVVETT
                     QVYTEEETRRAFEETQIDIPGGGSHGGSTMSLWEVMQSDLIPEEQRAQLMADFQAGRV
                     TKERMIIIIIEIIEKTEIIRQQGLASYDYVRRRLTAEDLFEARIISLETYNLLREGTR
                     SLREALEAESAWCYLYGTGSVAGVYLPGSRQTLSIYQALKKGLLSAEVARLLLEAQAA
                     TGFLLDPVKGERLTVDEAVRKGLVGPELHDRLLSAERAVTGYRDPYTEQTISLFQAMK
                     KELIPTEEALRLLDAQLATGGIVDPRLGFHLPLEVAYQRGYLNKDTHDQLSEPSEVRS
                     YVDPSTDERLSYTQLLRRCRRDDGTGQLLLPLSDARKLTFRGLRKQITMEELVRSQVM
                     DEATALQLREGLTSIEEVTKNLQKFLEGTSCIAGVFVDATKERLSVYQAMKKGIIRPG
                     TAFELLEAQAATGYVIDPIKGLKLTVEEAVRMGIVGPEFKDKLLSAERAVTGYKDPYS
                     GKLISLFQAMKKGLILKDHGIRLLEAQIATGGIIDPEESHRLPVEVAYKRGLFDEEMN
                     EILTDPSDDTKGFFDPNTEENLTYLQLMERCITDPQTGLCLLPLKEKKRERKTSSKSS
                     VRKRRVVIVDPETGKEMSVYEAYRKGLIDHQTYLELSEQECEWEEITISSSDGVVKSM
                     IIDRRSGRQYDIDDAIAKNLIDRSALDQYRAGTLSITEFADMLSGNAGGFRSRSSSVG
                     SSSSYPISPAVSRTQLASWSDPTEETGPVAGILDTETLEKVSITEAMHRNLVDNITGQ
                     RLLEAQACTGGIIDPSTGERFPVTDAVNKGLVDKIMVDRINLAQKAFCGFEDPRTKTK
                     MSAAQALKKGWLYYEAGQRFLEVQYLTGGLIEPDTPGRVPLDEALQRGTVDARTAQKL
                     RDVGAYSKYLTCPKTKLKISYKDALDRSMVEEGTGLRLLEAAAQSTKGYYSPYSVSGS
                     GSTAGSRTGSRTGSRAGSRRGSFDATGSGFSMTFSSSSYSSSGYGRRYASGSSASLGG
                     PESAVA"
     exon            243..304
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            305..394
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            395..472
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            473..565
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            566..732
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            733..848
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            849..955
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            956..1075
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1076..1171
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1172..1299
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1300..1393
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1394..1548
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1549..1867
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1868..1945
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1946..2107
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2108..2212
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2213..2308
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2309..2434
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2435..2587
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2588..2742
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2743..2869
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2870..3053
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3054..3211
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3212..3390
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3391..3529
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3530..3886
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3887..3970
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3971..4075
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            4076..4174
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            4175..7555
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            7556..14798
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      14778..14783
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /note="hexamer: AATAAA"
     polyA_site      14798
                     /gene="PLEC"
                     /gene_synonym="EBS1; EBS5A; EBS5B; EBS5C; EBS5D; EBSMD;
                     EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; LGMDR17; PCN;
                     PLEC1; PLEC1b; PLTN"
                     /note="major polyA site"
ORIGIN      
        1 agtctggctg cggcggcaga gctgaagtga gcggagccac caggaggcca tgtcgggtga
       61 ggacgctgag gtccgggcag tctctgaaga tgtctccaat ggaagcagtg gctcgcccag
      121 ccctggggac acactgccct ggaaccttgg gaaaacgcag cggagccggc gcagcggggg
      181 tggcgctggg agcaacggga gtgtcctgga cccagctgag cgggcggtca ttcgcatcgc
      241 agatgagcgg gatcgtgtgc agaagaaaac cttcaccaag tgggtcaaca agcacctcat
      301 caaggcccag aggcacatca gtgacctgta tgaagacctc cgcgatggcc acaacctcat
      361 ctccctgctg gaggtcctct cgggggacag cctgccccgg gagaagggga ggatgcgttt
      421 ccacaagctg cagaatgtcc agattgccct ggactacctc cggcaccgcc aggtgaagct
      481 ggtgaacatc aggaatgatg acatcgctga cggcaacccc aagctgaccc ttggcctcat
      541 ctggacaatc attctgcact tccagatctc agatatccag gtgagtgggc agtcggagga
      601 catgacggcc aaggagaagc tgctgctgtg gtcgcagcga atggtggagg ggtaccaggg
      661 cctgcgatgc gacaacttca cctccagctg gagagacggc cgcctcttca atgccatcat
      721 ccaccggcac aagcccctgc tcatcgacat gaacaaggtg taccggcaga ccaacctgga
      781 gaacctggac caggccttct ctgtggcgga gcgggacctg ggagtgacgc ggctcctgga
      841 ccctgaggac gtggatgtcc ctcagcccga cgagaagtcc atcatcacct acgtctcgtc
      901 gctgtatgac gccatgcccc gcgtgccgga cgtgcaggat ggggtgaggg ccaacgagct
      961 gcagctgcgc tggcaggagt accgggagct ggtgctgctg ctgcttcagt ggatgcgaca
     1021 ccacacggcc gcctttgagg aacgcaggtt cccctccagc ttcgaggaga ttgagatcct
     1081 gtggtctcag ttcctgaagt ttaaggagat ggagctacca gccaaggagg ccgacaagaa
     1141 caggtccaag ggcatctacc aatccctgga gggagcggtg caagcaggcc agctcaaggt
     1201 gccccctggc taccacccgc tggatgtgga gaaggagtgg ggcaagctgc acgtggccat
     1261 cctggagcgg gagaagcagc tccgcagcga gtttgagagg ctggagtgtc ttcagcgcat
     1321 cgtgaccaag ctgcagatgg aggcggggct gtgtgaggag cagctgaacc aggccgacgc
     1381 cctgctgcag tcggatgtcc ggctgctggc tgcaggcaaa gtgccacagc gggcggggga
     1441 ggtggaacgg gacttggaca aggcggatag catgatccgg ctgctcttca acgacgtgca
     1501 gaccctcaag gatggacggc acccgcaggg cgagcagatg taccgcaggg tgtaccgtct
     1561 gcacgagcgc ctggtagcca tccgcaccga gtacaaccta cggctgaagg caggcgtggc
     1621 ggcccctgca acccaggtgg cccaggtgac tctgcagagt gtgcagaggc gccccgagct
     1681 ggaggactcc actctgcgct acctgcagga cctgctggcc tgggtggagg agaaccagca
     1741 ccgtgtggat ggcgctgagt ggggtgtgga cctgcccagc gtggaggcgc agctgggcag
     1801 ccaccgaggc ctgcaccagt ccatcgaaga attccgggcc aagatcgagc gggcacggag
     1861 tgacgagggc cagctctccc ccgccacccg gggtgcctac cgtgactgcc tgggtcggct
     1921 ggacctgcag tacgccaagc tgctgaactc ctccaaggcc cgcctcaggt ccctggagag
     1981 cttgcacagc tttgtggcag ccgccactaa ggagctaatg tggctgaatg agaaggagga
     2041 ggaggaggtg ggcttcgact ggagcgaccg caacaccaac atgaccgcca agaaggagag
     2101 ctactcggcg ctgatgcggg agctggagct gaaggagaag aagatcaagg agctccaaaa
     2161 tgctggggac cggctgctgc gggaggacca cccggcccgg cccacggtgg agtccttcca
     2221 ggcggccctg cagacgcagt ggagctggat gctacagctg tgctgctgta tcgaggcaca
     2281 cctgaaggag aacgctgcct actttcagtt cttctcagat gtgcgggagg ccgaggggca
     2341 gttgcagaag ctgcaggagg cactgcgtag gaaatacagt tgtgatcgct ccgccaccgt
     2401 cacccggctg gaggacctgc tgcaggatgc ccaggacgag aaggaacagc tgaacgagta
     2461 caagggccac ctctcaggcc tggccaagcg ggccaaggcc gtcgtgcagc tgaagccccg
     2521 ccacccagcc caccccatgc ggggccgcct gcccctgctg gccgtgtgcg actataagca
     2581 ggtggaggtg actgtgcaca agggtgacga gtgccagctg gtgggccctg cacagccgtc
     2641 ccactggaag gtgctcagca gctccggcag cgaggccgcc gtgccctccg tgtgcttcct
     2701 ggtgcccccg cccaaccagg aggcccagga ggccgtcacc aggctggagg cccagcacca
     2761 ggccctggtc acgctgtggc accagttgca cgtggacatg aagagccttc tggcctggca
     2821 gagccttcgc cgcgacgtgc agctcatccg ctcctggtcc ctggccacgt tccgcaccct
     2881 gaagccagag gagcagcgcc aagccctgca cagcctggag ctgcactacc aggccttcct
     2941 gcgggacagc caggacgcgg gcggcttcgg acccgaggac cggctgatgg ctgagcgcga
     3001 gtacggctcc tgcagccacc actaccagca gctgctgcag agcctggaac agggtgcaca
     3061 ggaagagtct cgctgccagc gctgcatctc cgagctcaaa gacatccggc tgcagctgga
     3121 ggcctgtgag acgcgcaccg tgcaccgcct gcggctgccg ctggacaaag agccggcacg
     3181 ggagtgtgcc cagcgcatcg ccgagcagca gaaggcacag gcagaggtgg aggggctggg
     3241 caagggggtc gcccggctct ctgccgaggc cgagaaggtc ttggccctac cagagccatc
     3301 gcctgcggcc cccacgctgc gctcggagct ggagctgacg ctgggcaagc tggagcaggt
     3361 ccgcagcctg tctgccatct acctggagaa gctcaagacc atcagcctgg tgatccgcgg
     3421 cacgcagggg gccgaggagg tgctcagggc ccacgaggag cagctcaagg aggcccaggc
     3481 cgtgccggcc accctcccgg agctcgaggc caccaaggcc tctctgaaga agctgcgggc
     3541 ccaggccgag gcacagcagc ccacgttcga cgccctgcgg gatgagctgc ggggggcaca
     3601 ggaggtgggg gagcgactgc agcagcggca cggggagcgg gacgtggagg tggagcgctg
     3661 gcgggagcgg gtcgcccagt tgcttgagcg ctggcaggct gtgctggccc agaccgacgt
     3721 gcggcagcgc gagctcgagc aactgggccg ccagctgcgt tactaccgcg agagtgcaga
     3781 ccccttgggc gcctggctgc aggacgccag gcggcggcag gagcagatcc aggccatgcc
     3841 gctggccgac agccaggctg tgcgggagca gctgcggcag gagcaggccc tgctggagga
     3901 gatcgagcgc cacggcgaga aggtcgagga gtgccagagg tttgcgaaac agtacatcaa
     3961 cgccatcaag gactatgaac tccagctggt gacgtacaag gcgcagcttg agccggtggc
     4021 ctccccggcc aagaagccca aggtccagtc gggatcagag agtgtcatcc aggagtacgt
     4081 ggacctgcgt acgcactaca gcgagctgac cacactgacg agccagtaca tcaagttcat
     4141 cagcgagact ctgcggcgca tggaggagga ggagaggctg gctgagcagc agcgggcaga
     4201 ggagcgcgag cggctggccg aggtggaggc cgcgctggag aagcagcggc agctggccga
     4261 ggcgcacgcc caggcaaagg cacaggcgga gcgggaggcg aaggagctgc agcagcgcat
     4321 gcaggaggag gtggtgcggc gggaggaggc ggcggtggac gcgcagcagc agaagcgcag
     4381 cattcaggag gagctgcagc agctgcggca gagctcggag gcggagatcc aggccaaggc
     4441 ccggcaggca gaggcggctg agcgcagccg gctgcgcatc gaggaggaga tccgcgtggt
     4501 gcgcctgcag ttggaggcca ccgagcgcca gcgtggcggg gctgaggggg agctgcaggc
     4561 actgcgtgca cgggcggagg aggctgaggc acaaaagcga caggcgcagg aggaggccga
     4621 gcgcttgcgg aggcaggtgc aggacgagag ccagcgtaag cggcaggcgg aggtggagct
     4681 ggcctcgcgc gtgaaggccg aggccgaggc ggcgcgcgag aagcagcggg ccctgcaggc
     4741 cctggaggag ctgcggctgc aggcggagga ggcggagcgg cgcctgcggc aggccgaggt
     4801 ggagcgagcg cggcaggtac aggtggccct ggagacggcg cagcgcagtg cagaggcgga
     4861 gctgcagagc aaacgcgcct ccttcgccga gaagacggca cagctggagc gctccctgca
     4921 ggaggaacac gtggctgtgg cacagctgcg ggaggaggct gagcggcggg cacagcagca
     4981 ggccgaggcc gagcgggcgc gcgaggaggc agagcgggag ctggagcgct ggcagctcaa
     5041 ggccaacgag gcgctacggc tgcggctgca ggcggaggag gtggcgcagc agaagagcct
     5101 ggcgcaggcc gaggctgaga agcagaagga ggaggcggag cgcgaggcgc ggcggcgcgg
     5161 caaggcggag gagcaggccg tccggcagcg ggagctggct gaacaagagc tggagaagca
     5221 gcggcagctg gcggaaggca ccgcgcagca gcgcctggcc gcggagcagg agttgatccg
     5281 gctgcgggcc gagacggagc agggggagca gcagcggcag ctgctggagg aggagctggc
     5341 ccggctgcag cgtgaggcgg ctgcagccac gcagaaacgg caggagctgg aagccgagct
     5401 ggccaaggtg cgggccgaga tggaggtgct gctggccagc aaggcgaggg ctgaggagga
     5461 gtcgcgctcc accagcgaga agtccaagca gaggctggag gccgaggccg gccggttccg
     5521 cgagctggcc gaggaggccg cccgcctgcg tgccctggcg gaagaggcca agcggcagcg
     5581 gcagctggcc gaggaagacg cggcgcggca gcgggccgag gcggagcggg tgcttgcgga
     5641 gaagctggcc gccatcggcg aggccacgcg gctcaagacg gaggcggaga tcgcgctcaa
     5701 ggagaaggag gcggagaacg agcgcctgcg gcggctggcg gaggacgagg ccttccagcg
     5761 gcggcggctg gaggagcagg ccgcgcaaca caaggctgac atcgaggagc gcctggccca
     5821 gctgcgcaag gcatcggaca gcgagctgga gcggcagaag gggctggtgg aggacacgct
     5881 gaggcagcgg cggcaggtgg aggaggagat cctggcgctg aaggcgagct tcgagaaggc
     5941 ggccgctggc aaggcggagc tggagctgga gctgggacgc atccgcagca acgcggagga
     6001 cacgctgcgc agcaaggagc aggccgagct ggaggctgcg aggcagcggc agctggcggc
     6061 ggaggaggag cggcggcgcc gtgaggctga ggagcgcgtg cagaagagcc tggcggccga
     6121 ggaggaggcc gcacggcagc ggaaggcggc gctggaggaa gtcgagcggc tgaaagccaa
     6181 ggtggaggag gcgcggcgcc tgcgggagcg agcggagcag gagtcggcgc ggcagctgca
     6241 gctggcccag gaggccgccc agaagcggct gcaggcggaa gagaaggcac acgccttcgc
     6301 ggtgcagcag aaggagcagg agctacagca gacgctgcag caggagcaga gcgtgctgga
     6361 ccagctgcgc ggcgaggcgg aggcggcccg gcgggcggct gaggaggcgg aggaggcccg
     6421 ggtgcaggcg gagcgtgagg cggcgcagtc ccggcggcag gtggaagagg ccgagcggct
     6481 gaagcagtcg gcagaggagc aggcacaggc ccgggctcag gcacaggcgg ctgcagagaa
     6541 gctgcgcaag gaggccgagc aagaggcggc gcggcgggca caggcggagc aggcggccct
     6601 gcggcagaag caggcagctg acgcggagat ggagaagcat aagaaattcg ccgagcagac
     6661 gctgcggcag aaggcgcagg tggagcagga gctgacaaca ctgcggctgc agctggagga
     6721 gaccgaccac cagaagaacc tgctggacga ggagctgcag cggctgaagg cggaggccac
     6781 ggaggccgca cgccagcgca gccaggtgga ggaggagctc ttctcggtgc gcgtgcagat
     6841 ggaggagctg agcaagctca aggcacgcat cgaggctgag aaccgcgcac tcatcttgcg
     6901 tgacaaggac aatacgcagc gcttcctgca ggaggaggct gagaagatga agcaggtggc
     6961 ggaggaggcc gcgcggctga gtgtggcggc ccaagaggct gcgcgactgc ggcagctggc
     7021 agaggaggac ctggcacagc agcgggcctt ggcagagaag atgctcaagg agaagatgca
     7081 ggcggtgcag gaggccacgc gactcaaggc tgaggcggaa ctgctgcagc agcagaagga
     7141 gcttgcgcag gagcaggcgc ggcggctgca ggaggacaag gagcagatgg cgcagcagct
     7201 ggcggaggag acgcagggct tccagcggac gctggaggcc gagcggcagc ggcagctgga
     7261 gatgagcgct gaggctgagc gcctcaagct gcgtgtggcc gagatgagcc gagcccaggc
     7321 ccgcgctgag gaggacgccc agcgcttccg gaagcaggcg gaggagatcg gtgagaagct
     7381 gcaccgcacg gagctcgcca cccaggagaa ggtgaccctg gtgcagacac tggagatcca
     7441 gcgacagcag agtgaccatg atgccgagcg cctgcgggag gccatcgctg agctggagcg
     7501 tgagaaggag aagctccaac aggaggccaa actgctgcag ctcaagtctg aggagatgca
     7561 gacggtgcag caggagcagc tgctgcagga gacgcaggcc ctgcagcaaa gcttcctctc
     7621 tgaaaaggac agcctgctac agcgggagcg cttcatcgag caggagaagg ccaagctgga
     7681 gcagctcttc caggacgagg tggccaaggc acagcagctg cgtgaggagc agcagcggca
     7741 gcagcagcag atggagcagg aacggcagcg gctggtggcc agcatggagg aggcgcggcg
     7801 gcggcagcat gaggccgagg agggcgtgcg gcgcaagcag gaggagctgc agcagctgga
     7861 gcagcagcgg cggcagcagg aggagctgct ggctgaggag aaccagaggc tgcgtgagca
     7921 gctgcagctc ctggaggagc agcaccgggc cgcgctggcg cactcagagg aggtcactgc
     7981 ctcgcaggtg gctgccacaa agaccctgcc caatggccgg gatgcacttg atggccccgc
     8041 ggcagaggca gagccggagc acagcttcga tggcctgcgg cggaaggtgt cagctcagag
     8101 gctgcaggag gccggcatcc tgagtgcgga ggagctgcag cggttggcgc agggccacac
     8161 cacggtggac gagctcgcac ggcgggaaga cgtgcgccac tacctgcagg gccgcagcag
     8221 tatcgcaggg ctgttgctga aggccaccaa tgagaagctg agtgtttacg ccgccctgca
     8281 gaggcagctg ctgagtcccg gcacggccct catcctgctg gaggcgcagg cggcctcagg
     8341 cttcctgctg gaccctgtgc ggaaccggcg gctgaccgtc aacgaggctg tgaaggaggg
     8401 tgtggtgggc cccgagctgc accacaagct gctgtcggcc gagcgcgccg tcactggcta
     8461 caaggacccc tacactggcc agcagatctc tctcttccaa gccatgcaga agggcctcat
     8521 cgtccgggag cacggcatcc gcctgctgga ggcccagatc gccacgggcg gcgttatcga
     8581 ccccgtgcac agccaccgcg tgcccgtgga cgtggcctac cggcgcggct acttcgacga
     8641 ggagatgaac cgcgtcctgg cggaccccag cgacgacacc aagggcttct ttgaccccaa
     8701 cacgcacgag aacctcacgt acctgcagct actggagcgc tgcgtggagg accccgagac
     8761 gggcctgtgc cttctgccac tcacggataa ggctgccaag ggcggggagc tggtctacac
     8821 tgactccgag gcccgggacg tctttgagaa ggccaccgtg tctgcgccgt tcggcaagtt
     8881 ccagggcaag acggtgacca tttgggagat catcaactcg gaatacttca cggcagagca
     8941 gcggcgggac ctgctgcggc agttccgcac gggccggatc acagtggaga agatcatcaa
     9001 gatcatcatc acggtggtgg aggagcagga gcagaagggc cggctttgct ttgagggcct
     9061 gcgcagcctg gtgccagccg ccgagctgct ggagagcagg gtcatcgacc gcgagctcta
     9121 ccagcagctg cagcgaggtg agcgctctgt gcgagacgta gccgaggtgg acactgtgcg
     9181 gcgggctctc cggggtgcca acgtcatcgc gggtgtatgg ctggaggagg cggggcagaa
     9241 gctgagtatc tacaatgccc tgaagaaaga cctgctgcca tccgacatgg ccgtggccct
     9301 gttggaagcc caggccggca ccgggcacat catcgacccc gccaccagcg cccggctgac
     9361 cgtggacgag gcagtgcgtg ctggcctggt gggccccgag tttcatgaga agctgctatc
     9421 agccgagaag gctgtgacag ggtacaggga cccctacaca gggcagagcg tctccctgtt
     9481 ccaggccctg aagaagggcc tcattccccg ggagcagggc ctgcgcctgt tggacgccca
     9541 gctgtccacg ggcggcatcg tggaccccag caagagccac cgcgtgcccc tggatgtcgc
     9601 ctgcgcccga ggctgcctgg atgaggagac cagcagggcc ctgtcggcac caagggccga
     9661 cgccaaggcc tacagtgacc ccagcacagg ggagccggcc acctacggcg agctccagca
     9721 gcggtgccgg cccgaccagc tgaccgggct gagcctgctg ccgctctcag aaaaggctgc
     9781 tcgggcccgg caggaggagc tctactcaga gctgcaggcc cgtgagacct ttgaaaagac
     9841 cccggttgag gtccccgtgg gtggcttcaa gggcaggacg gtgacggtgt gggagctcat
     9901 cagctctgag tacttcactg cggagcagcg gcaggagctg ttgcgtcagt tccgcacggg
     9961 caaggtcacc gtggagaagg tcatcaagat tctcattacc atcgtggagg aggtggagac
    10021 cctgcggcag gagaggctgt ccttcagcgg cctccgtgcc cctgtgccag ccagcgagct
    10081 cctggcttcc ggggtcctca gcagagccca gtttgagcag ctcaaggacg gcaagacgac
    10141 ggtcaaggac ctttcggagc tgggctccgt gcggacgctg ctgcagggca gtggctgcct
    10201 cgccggcatc tacctggagg acaccaagga gaaggtgtcc atctacgagg ccatgcgccg
    10261 gggcctgctg agagccacaa cggctgcgct cctgctggag gcgcaggcgg ccactggctt
    10321 cctggtggac cccgtgcgga accagcgcct gtatgtccac gaggccgtga aggcgggcgt
    10381 ggtgggcccc gagcttcacg agcagctgct gtctgccgag aaggccgtca ccggctacag
    10441 agacccctac tcgggcagca ccatctccct cttccaggcc atgcagaagg gcctggttct
    10501 ccggcagcac ggcatccgcc tgctggaggc ccagatcgcc acgggcggca tcatcgaccc
    10561 cgtgcacagc caccgcgtgc ctgtggacgt ggcctaccag cgcggctact tcagtgagga
    10621 gatgaaccgc gtcctggcgg accccagcga cgacaccaag ggcttctttg accccaacac
    10681 gcatgagaac ctcacgtaca ggcagctgct ggagcggtgc gtggaggacc ccgagacggg
    10741 cttgcgcctt ctgccactga aaggggcgga gaaggctgag gtggtggaga ccacgcaggt
    10801 gtacactgag gaggagacaa gaagggcatt tgaagagaca cagatcgaca ttcccggcgg
    10861 cggcagccac ggcggctcca ccatgtccct gtgggaggtg atgcagtcgg acctgatccc
    10921 cgaggagcag cgggcccagc tgatggctga cttccaggcc ggccgggtga ccaaggaacg
    10981 catgatcatc atcatcatcg agatcattga gaagacagag atcatccgcc agcagggtct
    11041 ggcctcctac gactacgtgc gccgccgcct cacggctgag gacctgttcg aggctcggat
    11101 catctctctc gagacctaca acctgctccg ggagggcacc aggagcctcc gtgaggctct
    11161 cgaggcggag tccgcctggt gctacctcta tggcacgggc tccgtggctg gtgtctacct
    11221 gcccggttcc aggcagacac tgagcatcta ccaggctctc aagaaagggc tgctgagtgc
    11281 cgaggtggcc cgcctgctgc tggaggcaca ggcagccaca ggcttcctgc tggacccggt
    11341 gaagggggag cggctgactg tggatgaggc tgtgcggaag ggcctcgtgg ggcccgagct
    11401 gcacgaccgc ctgctctcgg ctgagcgggc ggtcaccggc taccgtgacc cctacaccga
    11461 gcagaccatc tcgctcttcc aggccatgaa gaaggagctg atccctactg aggaggccct
    11521 gcggctgctg gatgcccagc tggccaccgg cggcatcgtg gacccccgcc tgggcttcca
    11581 ccttcccctg gaggtggctt accagcgtgg ctacctcaac aaggacacgc acgaccagct
    11641 gtcagagccc agcgaggtgc gcagctacgt ggacccgtcc accgacgagc gcctcagcta
    11701 cacgcagctg ctcaggcggt gccgtcgtga cgacggcacc ggccagctgc tcctgccact
    11761 gtcggacgcc cgcaagctga ccttccgtgg cctgcggaag cagatcacca tggaggagct
    11821 ggtgcgctcg caggtcatgg acgaggccac ggcgctgcag ctgcgggagg gcctgacctc
    11881 catcgaggag gtcaccaaga acttgcagaa gttcctggaa ggcaccagct gcatcgctgg
    11941 tgtcttcgtg gacgccacca aggaacggct ctcggtgtac caggccatga agaagggcat
    12001 catccgcccc ggcacagcct ttgagctcct ggaggcgcag gcggccaccg gttacgtcat
    12061 cgaccccatc aagggactga agctgacggt ggaggaggct gtgcgtatgg gcattgtggg
    12121 ccccgagttc aaggacaagc tgctgtcggc cgagcgcgcc gtcactgggt acaaggaccc
    12181 ctactctggg aagctcatct ccctcttcca ggccatgaag aagggcctga tcctgaagga
    12241 ccatggcatc cgcctgctgg aggcccagat cgccacgggc ggcatcatcg accctgagga
    12301 gagccaccgg ctgcccgtgg aggtggccta caagcgcggc ctcttcgatg aggagatgaa
    12361 cgagatcctg accgacccct cggacgacac caagggcttc tttgacccta acacggagga
    12421 gaacctcacc tacctgcagc tgatggagcg ttgtatcact gacccccaga cgggcctgtg
    12481 tctcttgccg ctgaaggaga agaagcggga gcggaagacg tcctccaagt cctccgtgcg
    12541 caagcgccga gtggtcatcg tggaccccga gacgggcaag gagatgtcag tgtacgaggc
    12601 ctaccgcaag ggcctgattg accaccagac gtacctggag ctgtccgagc aggagtgcga
    12661 gtgggaggag atcaccatct cctcctcgga cggcgtggtc aagtccatga tcatcgaccg
    12721 ccgctccggg cgccagtacg acatcgatga tgccatcgcc aagaacctca tcgaccgctc
    12781 ggcactggac cagtaccgcg ccggcacgct ctccatcacc gagttcgccg acatgctctc
    12841 gggcaacgcc ggtggtttcc gctcccgttc ctcctcggtg ggatcctcct cctcctaccc
    12901 catcagcccc gccgtctcca ggacccagct ggcctcctgg tcagacccca ctgaggagac
    12961 gggccccgtg gctggcatcc tggacacgga gacgctggag aaggtgtcca tcaccgaggc
    13021 catgcaccgg aacctggtgg ataacatcac ggggcagcgg ctgctggagg cgcaggcctg
    13081 caccgggggc atcatcgacc ccagcaccgg tgagcgcttc cctgtcaccg acgccgtcaa
    13141 caagggcctg gtggacaaga tcatggtgga ccgcatcaac ctggcccaga aggccttctg
    13201 cggcttcgag gacccacgca ccaagaccaa gatgtcggcc gcccaggccc tgaagaaggg
    13261 ctggctctac tacgaggccg gccagcgctt cctggaggtg cagtacctga ccggcggctt
    13321 gatcgagccc gacacgccgg gccgcgtgcc cctggacgag gccctgcagc gcggcacggt
    13381 ggacgcccgc accgcacaga agctgcgtga cgtgggcgcc tactccaagt acctcacctg
    13441 ccctaagacc aagctcaaga tctcctataa ggacgcgctg gaccgcagca tggtggagga
    13501 gggcacgggg ctgcggctgc tggaggctgc cgcgcagtcc accaagggct actacagccc
    13561 ctacagcgtc agcggctccg gctctaccgc tggctcccgc accggctcgc gcaccggctc
    13621 ccgggccggc tcccgccgcg gcagctttga cgccaccggc tccggcttct ccatgacctt
    13681 ctcttcatcc tcctactcct cctcgggcta cggccgccgc tacgcctcgg ggtcctcggc
    13741 ctccctgggg ggccctgagt ctgccgtggc ctgaggctgc ctgcgcccac cccgctctgc
    13801 atgcggccca gcccggctcc caccgaggcg cgggggccgt tttcaacgct taaaggtgtc
    13861 ttcctcccaa gtggtgccta aagtttaacc aaaaagacca gactaatata ttaatatata
    13921 tctgctgtcc agacagcctg tatcttgggg gacagggctg gcccagccct gctggccgcc
    13981 tcaccccctc gggtctcctc actcccttct acctgccact cacacagcca ggtgccttgg
    14041 agggtcccaa gctgggcccc agcccaccct cctgtcttcc cagggtagcc cgcctgccag
    14101 tcctagctgc acagggcagc tgggcccaac cctgtctgta gagggccctg gtgtttctag
    14161 cactggcctg cacggtgggc cttgctgggg acggggggcc ccagtcagcc tctctcccag
    14221 tctacccaga gaagcccctt ccccatggga agacgaggcc ctcgggccca gcccccacag
    14281 tgctgtctga tctgtgcttt ccagctcacc ccccacactc actcctgaga cccctggcct
    14341 ccggcgtcag cctccagcct ctgttcccct agtaagtgcc ttccatgtcg gcctctaacc
    14401 ccaggccccg aggacccaga cccagtgggg aggcggacgt tccagccggc atggctggga
    14461 actgcagacc tgtcctcctg gtgggtccag gggcccctcc agcttgtgga gccccacact
    14521 ggggtgccgc ctgcccgtct ctctcccatg gagccccagc cccctttggg cccagggaca
    14581 ccagccaggc tctgtgctga ccctcctgtt gcacccagcc ctggtctcag cagcgaccac
    14641 ccctgcctcc accctctgag ctttgcatgt tccactaacc ccgggcgggt ggcaggtgga
    14701 ggtgtcaggc tgctggcgcc tctgcaaggg cagaacacta acctgaccgt gggcggggcc
    14761 ttgcggtatc cgcccccaat aaaagcaatt ccaacctt
//
