LOCUS       NM_002663               3443 bp    mRNA    linear   PRI 23-APR-2024
DEFINITION  Homo sapiens phospholipase D2 (PLD2), transcript variant 1, mRNA.
ACCESSION   NM_002663
VERSION     NM_002663.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Munoz-Galvan,S., Verdugo-Sivianes,E.M., Santos-Pereira,J.M.,
            Estevez-Garcia,P. and Carnero,A.
  TITLE     Essential role of PLD2 in hypoxia-induced stemness and therapy
            resistance in ovarian tumors
  JOURNAL   J Exp Clin Cancer Res 43 (1), 57 (2024)
   PUBMED   38403587
  REMARK    GeneRIF: Essential role of PLD2 in hypoxia-induced stemness and
            therapy resistance in ovarian tumors.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Qian,T., Qi,B., Fei,Y., Li,J., Luo,L., Lv,B., Song,Y., Sheng,S.,
            Xiao,W., Huang,X. and Wang,X.
  TITLE     PLD2 deletion alleviates disruption of tight junctions in
            sepsis-induced ALI by regulating PA/STAT3 phosphorylation pathway
  JOURNAL   Int Immunopharmacol 114, 109561 (2023)
   PUBMED   36700766
  REMARK    GeneRIF: PLD2 deletion alleviates disruption of tight junctions in
            sepsis-induced ALI by regulating PA/STAT3 phosphorylation pathway.
REFERENCE   3  (bases 1 to 3443)
  AUTHORS   Wolf,A., Tanguy,E., Wang,Q., Gasman,S. and Vitale,N.
  TITLE     Phospholipase D and cancer metastasis: A focus on exosomes
  JOURNAL   Adv Biol Regul 87, 100924 (2023)
   PUBMED   36272918
  REMARK    GeneRIF: Phospholipase D and cancer metastasis: A focus on
            exosomes.
            Review article
REFERENCE   4  (bases 1 to 3443)
  AUTHORS   Onallah,H., Mannully,S.T., Davidson,B. and Reich,R.
  TITLE     Exosome Secretion and Epithelial-Mesenchymal Transition in Ovarian
            Cancer Are Regulated by Phospholipase D
  JOURNAL   Int J Mol Sci 23 (21), 13286 (2022)
   PUBMED   36362078
  REMARK    GeneRIF: Exosome Secretion and Epithelial-Mesenchymal Transition in
            Ovarian Cancer Are Regulated by Phospholipase D.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3443)
  AUTHORS   Lee,H., Jung,T.Y., Lim,S.H., Choi,E.J., Lee,J. and Min,D.S.
  TITLE     Phospholipase D2 is a positive regulator of sirtuin 1 and modulates
            p53-mediated apoptosis via sirtuin 1
  JOURNAL   Exp Mol Med 53 (9), 1287-1297 (2021)
   PUBMED   34471223
  REMARK    GeneRIF: Phospholipase D2 is a positive regulator of sirtuin 1 and
            modulates p53-mediated apoptosis via sirtuin 1.
REFERENCE   6  (bases 1 to 3443)
  AUTHORS   Slaaby,R., Jensen,T., Hansen,H.S., Frohman,M.A. and Seedorf,K.
  TITLE     PLD2 complexes with the EGF receptor and undergoes tyrosine
            phosphorylation at a single site upon agonist stimulation
  JOURNAL   J Biol Chem 273 (50), 33722-33727 (1998)
   PUBMED   9837959
REFERENCE   7  (bases 1 to 3443)
  AUTHORS   Steed,P.M., Clark,K.L., Boyar,W.C. and Lasala,D.J.
  TITLE     Characterization of human PLD2 and the analysis of PLD isoform
            splice variants
  JOURNAL   FASEB J 12 (13), 1309-1317 (1998)
   PUBMED   9761774
REFERENCE   8  (bases 1 to 3443)
  AUTHORS   Lopez,I., Arnold,R.S. and Lambeth,J.D.
  TITLE     Cloning and initial characterization of a human phospholipase D2
            (hPLD2). ADP-ribosylation factor regulates hPLD2
  JOURNAL   J Biol Chem 273 (21), 12846-12852 (1998)
   PUBMED   9582313
REFERENCE   9  (bases 1 to 3443)
  AUTHORS   Park,S.H., Ryu,S.H., Suh,P.G. and Kim,H.
  TITLE     Assignment of human PLD2 to chromosome band 17p13.1 by fluorescence
            in situ hybridization
  JOURNAL   Cytogenet Cell Genet 82 (3-4), 225 (1998)
   PUBMED   9858823
REFERENCE   10 (bases 1 to 3443)
  AUTHORS   Colley,W.C., Sung,T.C., Roll,R., Jenco,J., Hammond,S.M.,
            Altshuller,Y., Bar-Sagi,D., Morris,A.J. and Frohman,M.A.
  TITLE     Phospholipase D2, a distinct phospholipase D isoform with novel
            regulatory properties that provokes cytoskeletal reorganization
  JOURNAL   Curr Biol 7 (3), 191-201 (1997)
   PUBMED   9395408
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC407587.1, AF033850.2,
            AF038440.1 and BC015033.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_002663.4.
            
            Summary: The protein encoded by this gene catalyzes the hydrolysis
            of phosphatidylcholine to phosphatidic acid and choline. The
            activity of the encoded enzyme is enhanced by phosphatidylinositol
            4,5-bisphosphate and ADP-ribosylation factor-1. This protein
            localizes to the peripheral membrane and may be involved in
            cytoskeletal organization, cell cycle control, transcriptional
            regulation, and/or regulated secretion. Two transcript variants
            encoding different isoforms have been found for this gene.[provided
            by RefSeq, Jul 2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (PLD2A).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF038440.1, BC015033.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000263088.11/ ENSP00000263088.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-458               DC407587.1         28-485
            459-588             AF033850.2         496-625
            589-877             AF038440.1         598-886
            878-1253            AF033850.2         915-1290
            1254-1771           AF038440.1         1263-1780
            1772-3201           AF033850.2         1809-3238
            3202-3443           BC015033.1         3214-3455
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p13.2"
     gene            1..3443
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /note="phospholipase D2"
                     /db_xref="GeneID:5338"
                     /db_xref="HGNC:HGNC:9068"
                     /db_xref="MIM:602384"
     exon            1..74
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            75..184
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     CDS             76..2877
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /EC_number="3.1.4.4"
                     /note="isoform PLD2A is encoded by transcript variant 1;
                     choline phosphatase 2; phosphatidylcholine-hydrolyzing
                     phospholipase D2"
                     /codon_start=1
                     /product="phospholipase D2 isoform PLD2A"
                     /protein_id="NP_002654.3"
                     /db_xref="CCDS:CCDS11057.1"
                     /db_xref="GeneID:5338"
                     /db_xref="HGNC:HGNC:9068"
                     /db_xref="MIM:602384"
                     /translation="MTATPESLFPTGDELDSSQLQMESDEVDTLKEGEDPADRMHPFL
                     AIYELQSLKVHPLVFAPGVPVTAQVVGTERYTSGSKVGTCTLYSVRLTHGDFSWTTKK
                     KYRHFQELHRDLLRHKVLMSLLPLARFAVAYSPARDAGNREMPSLPRAGPEGSTRHAA
                     SKQKYLENYLNRLLTMSFYRNYHAMTEFLEVSQLSFIPDLGRKGLEGMIRKRSGGHRV
                     PGLTCCGRDQVCYRWSKRWLVVKDSFLLYMCLETGAISFVQLFDPGFEVQVGKRSTEA
                     RHGVRIDTSHRSLILKCSSYRQARWWAQEITELAQGPGRDFLQLHRHDSYAPPRPGTL
                     ARWFVNGAGYFAAVADAILRAQEEIFITDWWLSPEVYLKRPAHSDDWRLDIMLKRKAE
                     EGVRVSILLFKEVELALGINSGYSKRALMLLHPNIKVMRHPDQVTLWAHHEKLLVVDQ
                     VVAFLGGLDLAYGRWDDLHYRLTDLGDSSESAASQPPTPRPDSPATPDLSHNQFFWLG
                     KDYSNLITKDWVQLDRPFEDFIDRETTPRMPWRDVGVVVHGLPARDLARHFIQRWNFT
                     KTTKAKYKTPTYPYLLPKSTSTANQLPFTLPGGQCTTVQVLRSVDRWSAGTLENSILN
                     AYLHTIRESQHFLYIENQFFISCSDGRTVLNKVGDEIVDRILKAHKQGWCYRVYVLLP
                     LLPGFEGDISTGGGNSIQAILHFTYRTLCRGEYSILHRLKAAMGTAWRDYISICGLRT
                     HGELGGHPVSELIYIHSKVLIADDRTVIIGSANINDRSLLGKRDSELAVLIEDTETEP
                     SLMNGAEYQAGRFALSLRKHCFGVILGANTRPDLDLRDPICDDFFQLWQDMAESNANI
                     YEQIFRCLPSNATRSLRTLREYVAVEPLATVSPPLARSELTQVQGHLVHFPLKFLEDE
                     SLLPPLGSKEGMIPLEVWT"
     exon            185..315
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            316..458
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            459..564
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            565..630
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            631..689
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            690..782
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            783..935
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            936..1085
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1086..1169
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1170..1248
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1249..1359
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1360..1530
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1531..1657
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1658..1776
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1777..1890
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1891..1995
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1996..2084
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            2085..2198
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            2199..2248
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            2249..2383
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            2384..2537
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            2538..2652
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            2653..3443
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3425..3430
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /note="hexamer: AATAAA"
     polyA_site      3443
                     /gene="PLD2"
                     /gene_synonym="PLD1C"
                     /note="major polyA site"
ORIGIN      
        1 gtgggctccg gctgcagctc cggtctgctc tcttggctcc ggaacccccg cgggcgctgg
       61 ctccgtctgc cagggatgac ggcgacccct gagagcctct tccccactgg ggacgaactg
      121 gactccagcc agctccagat ggagtccgat gaggtggaca ccctgaagga gggagaggac
      181 ccagccgacc ggatgcaccc gtttctggcc atctatgagc ttcagtctct gaaagtgcac
      241 cccttggtgt tcgcacctgg ggtccctgtc acagcccagg tggtgggcac cgaaagatat
      301 accagcggat ccaaggtggg aacctgcact ctgtattctg tccgcttgac tcacggcgac
      361 ttttcctgga caaccaagaa gaaataccgt cattttcagg agctgcatcg ggacctcctg
      421 agacacaaag tcttgatgag tctgctccct ctggctcgat ttgccgttgc ctattctcca
      481 gcccgagatg caggcaacag agagatgccc tctctacccc gggcaggtcc tgagggctcc
      541 accagacatg cagccagcaa acagaaatac ctggagaatt acctcaaccg tctcttgacc
      601 atgtctttct atcgcaacta ccatgccatg acagagttcc tggaagtcag tcagctgtcc
      661 tttatcccgg acttgggccg caaaggactg gaggggatga tccggaagcg ctcaggtggc
      721 caccgtgttc ctggcctcac ctgctgtggc cgagaccaag tttgttatcg ctggtccaag
      781 aggtggctgg tggtgaagga ctccttcctg ctgtacatgt gcctcgagac aggtgccatc
      841 tcatttgttc agctctttga ccctggcttt gaggtgcaag tggggaaaag gagcacggag
      901 gcacggcacg gcgtgcggat cgatacctcc cacaggtcct tgattctcaa gtgcagcagc
      961 taccggcagg cacggtggtg ggcccaagag atcactgagc tggcacaggg cccaggcaga
     1021 gacttcctac agctgcaccg gcatgacagc tacgccccac cccggcctgg gaccttggcc
     1081 cggtggtttg tgaatggggc aggttacttt gctgctgtgg cagatgccat ccttcgagct
     1141 caagaggaga ttttcatcac agactggtgg ttgagtcctg aggtttacct gaagcgtccg
     1201 gcccattcag atgactggag actggacatt atgctcaaga ggaaggcgga ggagggtgtc
     1261 cgtgtgtcta ttctgctgtt taaagaagtg gaattggcct tgggcatcaa cagtggctat
     1321 agcaagaggg cgctgatgct gctgcacccc aacataaagg tgatgcgtca cccagaccaa
     1381 gtgacgttgt gggcccatca tgagaagctc ctggtggtgg accaagtggt agcattcctg
     1441 gggggactgg accttgccta tggccgctgg gatgacctgc actaccgact gactgacctt
     1501 ggagactcct ctgaatcagc tgcctcccag cctcccaccc cgcgcccaga ctcaccagcc
     1561 accccagacc tctctcacaa ccaattcttc tggctgggca aggactacag caatcttatc
     1621 accaaggact gggtgcagct ggaccggcct ttcgaagatt tcattgacag ggagacgacc
     1681 cctcggatgc catggcggga cgttggggtg gtcgtccatg gcctaccggc ccgggacctt
     1741 gcccggcact tcatccagcg ctggaacttc accaagacca ccaaggccaa gtacaagact
     1801 cccacatacc cctacctgct tcccaagtct accagcacgg ccaatcagct ccccttcaca
     1861 cttccaggag ggcagtgcac caccgtacag gtcttgcgat cagtggaccg ctggtcagca
     1921 gggactctgg agaactccat cctcaatgcc tacctgcaca ccatcaggga gagccagcac
     1981 ttcctctaca ttgagaatca gttcttcatt agctgctcag atgggcggac ggttctgaac
     2041 aaggtgggcg atgagattgt ggacagaatc ctgaaggccc acaaacaggg gtggtgttac
     2101 cgagtctacg tgcttttgcc cttactccct ggcttcgagg gtgacatctc cacgggcggt
     2161 ggcaactcca tccaggccat tctgcacttt acttacagga ccctgtgtcg tggggagtat
     2221 tcaatcctgc atcgccttaa agcagccatg gggacagcat ggcgggacta tatttccatc
     2281 tgcgggcttc gtacacacgg agagctgggc gggcaccccg tctcggagct catctacatc
     2341 cacagcaagg tgctcatcgc agatgaccgg acagtcatca ttggttctgc aaacatcaat
     2401 gaccggagct tgctggggaa gcgggacagt gagctggccg tgctgatcga ggacacagag
     2461 acggaaccat ccctcatgaa tggggcagag tatcaggcgg gcaggtttgc cttgagtctg
     2521 cggaagcact gcttcggtgt gattcttgga gcaaataccc ggccagactt ggatctccga
     2581 gaccccatct gtgatgactt cttccagttg tggcaagaca tggctgagag caacgccaat
     2641 atctatgagc agatcttccg ctgcctgcca tccaatgcca cgcgttccct gcggactctc
     2701 cgggagtacg tggccgtgga gcccttggcc acggtcagtc cccccttggc tcggtctgag
     2761 ctcacccagg tccagggcca cctggtccac ttccccctca agttcctaga ggatgagtct
     2821 ttgctgcccc cgctgggtag caaggagggc atgatccccc tagaagtgtg gacatagttg
     2881 aggcccccgt cagggagagg tcaccagctg ctgtgcccca ccacgtctgg ctccctgccc
     2941 cttaacccca aggactgagg gcagtgccct ttgagatctg gggaggcagg cattcctgaa
     3001 gggaactaga ggtgttacag aggaccctta cgtgagaaat agctgaaaag ggcactccca
     3061 accctgggct ggggaggagg agagagtccc agagctcatc ccccctgctg cccagtgcaa
     3121 accacttctc catgctgcaa aggagaagca cagctcctgc cagggtgagc agggtcaagc
     3181 ctcttattcc aggagaaggg ggctctgccc caggccctac tacccattgt tcccttcctc
     3241 ttcctgccct tgaaccccct ccctgtccca gggccctccc agcccattgc tgccaaggtg
     3301 gagggaagga taaagccact tctggcttca gcccccacca ggggaaggaa ggagggcaca
     3361 ttaactccct ccaccagcct gctgacagac actaactttg tatccgttca ataagcattt
     3421 cataaataaa ggtgtagaaa agg
//
