LOCUS       NM_002662               6015 bp    mRNA    linear   PRI 24-OCT-2024
DEFINITION  Homo sapiens phospholipase D1 (PLD1), transcript variant 1, mRNA.
ACCESSION   NM_002662
VERSION     NM_002662.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Zhou,Y.Q., Cheng,X.X., He,S., Liu,S.Q., Li,Y.Q., Wei,P.P., Luo,C.L.
            and Bei,J.X.
  TITLE     A positive feedback loop between PLD1 and NF-kappaB signaling
            promotes tumorigenesis of nasopharyngeal carcinoma
  JOURNAL   J Genet Genomics 51 (10), 997-1006 (2024)
   PUBMED   38885836
  REMARK    GeneRIF: A positive feedback loop between PLD1 and NF-kappaB
            signaling promotes tumorigenesis of nasopharyngeal carcinoma.
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   Mahmud,S., Hamza,A., Lee,Y.B., Min,J.K., Islam,R., Dogsom,O. and
            Park,J.B.
  TITLE     Lipopolysaccharide Stimulates A549 Cell Migration through p-Tyr 42
            RhoA and Phospholipase D1 Activity
  JOURNAL   Biomolecules 14 (1), 6 (2023)
   PUBMED   38275747
  REMARK    GeneRIF: Lipopolysaccharide Stimulates A549 Cell Migration through
            p-Tyr 42 RhoA and Phospholipase D1 Activity.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6015)
  AUTHORS   Fan,W.T., Liu,X.F. and Liang,R.C.
  TITLE     Raf/MEK/ERK Signaling Pathway Is Involved in the Inhibition of
            Glioma Cell Proliferation and Invasion in the Ketogenic
            Microenvironment
  JOURNAL   Curr Med Sci 43 (4), 759-767 (2023)
   PUBMED   37498407
  REMARK    GeneRIF: Raf/MEK/ERK Signaling Pathway Is Involved in the
            Inhibition of Glioma Cell Proliferation and Invasion in the
            Ketogenic Microenvironment.
REFERENCE   4  (bases 1 to 6015)
  AUTHORS   Fu,D., Yan,J., Zhang,Z., Liu,Y., Ma,X., Ding,J., Yang,S., Zhao,R.,
            Chang,A., Gao,C., Liu,J., Zhao,T., Wang,X., Huang,C., Gao,S.,
            Ma,Y., Tang,B., Feng,Y., Wang,H. and Hao,J.
  TITLE     Nuclear PLD1 combined with NPM1 induces gemcitabine resistance
            through tumorigenic IL7R in pancreatic adenocarcinoma
  JOURNAL   Cancer Biol Med 20 (8), 599-626 (2023)
   PUBMED   37381714
  REMARK    GeneRIF: Nuclear PLD1 combined with NPM1 induces gemcitabine
            resistance through tumorigenic IL7R in pancreatic adenocarcinoma.
REFERENCE   5  (bases 1 to 6015)
  AUTHORS   Onallah,H., Mannully,S.T., Davidson,B. and Reich,R.
  TITLE     Exosome Secretion and Epithelial-Mesenchymal Transition in Ovarian
            Cancer Are Regulated by Phospholipase D
  JOURNAL   Int J Mol Sci 23 (21), 13286 (2022)
   PUBMED   36362078
  REMARK    GeneRIF: Exosome Secretion and Epithelial-Mesenchymal Transition in
            Ovarian Cancer Are Regulated by Phospholipase D.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6015)
  AUTHORS   Bae,C.D., Min,D.S., Fleming,I.N. and Exton,J.H.
  TITLE     Determination of interaction sites on the small G protein RhoA for
            phospholipase D
  JOURNAL   J Biol Chem 273 (19), 11596-11604 (1998)
   PUBMED   9565577
REFERENCE   7  (bases 1 to 6015)
  AUTHORS   Luo,J.Q., Liu,X., Hammond,S.M., Colley,W.C., Feig,L.A.,
            Frohman,M.A., Morris,A.J. and Foster,D.A.
  TITLE     RalA interacts directly with the Arf-responsive, PIP2-dependent
            phospholipase D1
  JOURNAL   Biochem Biophys Res Commun 235 (3), 854-859 (1997)
   PUBMED   9207251
REFERENCE   8  (bases 1 to 6015)
  AUTHORS   Colley,W.C., Sung,T.C., Roll,R., Jenco,J., Hammond,S.M.,
            Altshuller,Y., Bar-Sagi,D., Morris,A.J. and Frohman,M.A.
  TITLE     Phospholipase D2, a distinct phospholipase D isoform with novel
            regulatory properties that provokes cytoskeletal reorganization
  JOURNAL   Curr Biol 7 (3), 191-201 (1997)
   PUBMED   9395408
REFERENCE   9  (bases 1 to 6015)
  AUTHORS   Hammond,S.M., Jenco,J.M., Nakashima,S., Cadwallader,K., Gu,Q.,
            Cook,S., Nozawa,Y., Prestwich,G.D., Frohman,M.A. and Morris,A.J.
  TITLE     Characterization of two alternately spliced forms of phospholipase
            D1. Activation of the purified enzymes by phosphatidylinositol
            4,5-bisphosphate, ADP-ribosylation factor, and Rho family monomeric
            GTP-binding proteins and protein kinase C-alpha
  JOURNAL   J Biol Chem 272 (6), 3860-3868 (1997)
   PUBMED   9013646
REFERENCE   10 (bases 1 to 6015)
  AUTHORS   Hammond,S.M., Altshuller,Y.M., Sung,T.C., Rudge,S.A., Rose,K.,
            Engebrecht,J., Morris,A.J. and Frohman,M.A.
  TITLE     Human ADP-ribosylation factor-activated
            phosphatidylcholine-specific phospholipase D defines a new and
            highly conserved gene family
  JOURNAL   J Biol Chem 270 (50), 29640-29643 (1995)
   PUBMED   8530346
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA464847.1, BC068976.1,
            AB209907.1 and AC008134.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_002662.4.
            
            Summary: This gene encodes a phosphatidylcholine-specific
            phospholipase which catalyzes the hydrolysis of phosphatidylcholine
            in order to yield phosphatidic acid and choline. The enzyme may
            play a role in signal transduction and subcellular trafficking.
            Alternative splicing results in multiple transcript variants with
            both catalytic and regulatory properties. [provided by RefSeq, Sep
            2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC068976.1, SRR11853561.21542.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000351298.9/ ENSP00000342793.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-38                DA464847.1         12-49
            39-5593             BC068976.1         1-5555
            5594-6014           AB209907.1         5435-5855
            6015-6015           AC008134.3         115979-115979       c
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q26.31"
     gene            1..6015
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /note="phospholipase D1"
                     /db_xref="GeneID:5337"
                     /db_xref="HGNC:HGNC:9067"
                     /db_xref="MIM:602382"
     exon            1..85
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     exon            86..276
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             117..3341
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /EC_number="3.1.4.4"
                     /note="isoform a is encoded by transcript variant 1;
                     choline phosphatase 1; phospholipase D1,
                     phosphatidylcholine-specific;
                     phosphatidylcholine-hydrolyzing phospholipase D1"
                     /codon_start=1
                     /product="phospholipase D1 isoform a"
                     /protein_id="NP_002653.1"
                     /db_xref="CCDS:CCDS3216.1"
                     /db_xref="GeneID:5337"
                     /db_xref="HGNC:HGNC:9067"
                     /db_xref="MIM:602382"
                     /translation="MSLKNEPRVNTSALQKIAADMSNIIENLDTRELHFEGEEVDYDV
                     SPSDPKIQEVYIPFSAIYNTQGFKEPNIQTYLSGCPIKAQVLEVERFTSTTRVPSINL
                     YTIELTHGEFKWQVKRKFKHFQEFHRELLKYKAFIRIPIPTRRHTFRRQNVREEPREM
                     PSLPRSSENMIREEQFLGRRKQLEDYLTKILKMPMYRNYHATTEFLDISQLSFIHDLG
                     PKGIEGMIMKRSGGHRIPGLNCCGQGRACYRWSKRWLIVKDSFLLYMKPDSGAIAFVL
                     LVDKEFKIKVGKKETETKYGIRIDNLSRTLILKCNSYRHARWWGGAIEEFIQKHGTNF
                     LKDHRFGSYAAIQENALAKWYVNAKGYFEDVANAMEEANEEIFITDWWLSPEIFLKRP
                     VVEGNRWRLDCILKRKAQQGVRIFIMLYKEVELALGINSEYTKRTLMRLHPNIKVMRH
                     PDHVSSTVYLWAHHEKLVIIDQSVAFVGGIDLAYGRWDDNEHRLTDVGSVKRVTSGPS
                     LGSLPPAAMESMESLRLKDKNEPVQNLPIQKSIDDVDSKLKGIGKPRKFSKFSLYKQL
                     HRHHLHDADSISSIDSTSSYFNHYRSHHNLIHGLKPHFKLFHPSSESEQGLTRPHADT
                     GSIRSLQTGVGELHGETRFWHGKDYCNFVFKDWVQLDKPFADFIDRYSTPRMPWHDIA
                     SAVHGKAARDVARHFIQRWNFTKIMKSKYRSLSYPFLLPKSQTTAHELRYQVPGSVHA
                     NVQLLRSAADWSAGIKYHEESIHAAYVHVIENSRHYIYIENQFFISCADDKVVFNKIG
                     DAIAQRILKAHRENQKYRVYVVIPLLPGFEGDISTGGGNALQAIMHFNYRTMCRGENS
                     ILGQLKAELGNQWINYISFCGLRTHAELEGNLVTELIYVHSKLLIADDNTVIIGSANI
                     NDRSMLGKRDSEMAVIVQDTETVPSVMDGKEYQAGRFARGLRLQCFRVVLGYLDDPSE
                     DIQDPVSDKFFKEVWVSTAARNATIYDKVFRCLPNDEVHNLIQLRDFINKPVLAKEDP
                     IRAEEELKKIRGFLVQFPFYFLSEESLLPSVGTKEAIVPMEVWT"
     exon            277..404
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     exon            405..550
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     exon            551..656
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     exon            657..722
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     exon            723..781
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     exon            782..874
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     exon            875..1027
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1028..1177
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..1261
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1262..1343
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1344..1454
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1455..1655
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1656..1869
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1870..1983
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1984..2112
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2113..2231
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2232..2345
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2346..2456
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2457..2545
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2546..2659
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2660..2709
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2710..2844
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2845..2998
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2999..3116
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3117..6015
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3593..3598
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /note="hexamer: AGTAAA"
     polyA_site      3618
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /note="major polyA site"
     regulatory      5556..5561
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /note="hexamer: AATAAA"
     regulatory      5560..5565
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /note="hexamer: AACAAG"
     polyA_site      5577
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /note="major polyA site"
     polyA_site      5593
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /note="major polyA site"
     regulatory      5996..6001
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
                     /note="hexamer: TATAAA"
     polyA_site      6015
                     /gene="PLD1"
                     /gene_synonym="CVDD; CVDP1"
ORIGIN      
        1 gctgagctcc agctgtgcca gaggcacccg agccctgaga gtccgccgcc aacgcgcagg
       61 tgctagcggc cccttcgccc tgcagcccct ttgcttttac tctgtccaaa gttaacatgt
      121 cactgaaaaa cgagccacgg gtaaatacct ctgcactgca gaaaattgct gctgacatga
      181 gtaatatcat agaaaatctg gacacgcggg aactccactt tgagggagag gaggtagact
      241 acgacgtgtc tcccagcgat cccaagatac aagaagtgta tatccctttc tctgctattt
      301 ataacactca aggatttaag gagcctaata tacagacgta tctctccggc tgtccaataa
      361 aagcacaagt tctggaagtg gaacgcttca catctacaac aagggtacca agtattaatc
      421 tttacactat tgaattaaca catggggaat ttaaatggca agttaagagg aaattcaagc
      481 attttcaaga atttcacaga gagctgctca agtacaaagc ctttatccgc atccccattc
      541 ccactagaag acacacgttt aggaggcaaa acgtcagaga ggagcctcga gagatgccca
      601 gtttgccccg ttcatctgaa aacatgataa gagaagaaca attccttggt agaagaaaac
      661 aactggaaga ttacttgaca aagatactaa aaatgcccat gtatagaaac tatcatgcca
      721 caacagagtt tcttgatata agccagctgt ctttcatcca tgatttggga ccaaagggca
      781 tagaaggtat gataatgaaa agatctggag gacacagaat accaggcttg aattgctgtg
      841 gtcagggaag agcctgctac agatggtcaa aaagatggtt aatagtgaaa gattcctttt
      901 tattgtatat gaaaccagac agcggtgcca ttgccttcgt cctgctggta gacaaagaat
      961 tcaaaattaa ggtggggaag aaggagacag aaacgaaata tggaatccga attgataatc
     1021 tttcaaggac acttatttta aaatgcaaca gctatagaca tgctcggtgg tggggagggg
     1081 ctatagaaga attcatccag aaacatggca ccaactttct caaagatcat cgatttgggt
     1141 catatgctgc tatccaagag aatgctttag ctaaatggta tgttaatgcc aaaggatatt
     1201 ttgaagatgt ggcaaatgca atggaagagg caaatgaaga gatttttatc acagactggt
     1261 ggctgagtcc agaaatcttc ctgaaacgcc cagtggttga gggaaatcgt tggaggttgg
     1321 actgcattct taaacgaaaa gcacaacaag gagtgaggat cttcataatg ctctacaaag
     1381 aggtggaact cgctcttggc atcaatagtg aatacaccaa gaggactttg atgcgtctac
     1441 atcccaacat aaaggtgatg agacacccgg atcatgtgtc atccaccgtc tatttgtggg
     1501 ctcaccatga gaagcttgtc atcattgacc aatcggtggc ctttgtggga gggattgacc
     1561 tggcctatgg aaggtgggac gacaatgagc acagactcac agacgtgggc agtgtgaagc
     1621 gggtcacttc aggaccgtct ctgggttccc tcccacctgc cgcaatggag tctatggaat
     1681 ccttaagact caaagataaa aatgagcctg ttcaaaacct acccatccag aagagtattg
     1741 atgatgtgga ttcaaaactg aaaggaatag gaaagccaag aaagttctcc aaatttagtc
     1801 tctacaagca gctccacagg caccacctgc acgacgcaga tagcatcagc agcattgaca
     1861 gcacctccag ttattttaat cactatagaa gtcatcacaa tttaatccat ggtttaaaac
     1921 cccacttcaa actctttcac ccgtccagtg agtctgagca aggactcact agacctcatg
     1981 ctgataccgg gtccatccgt agtttacaga caggtgtggg agagctgcat ggggaaacca
     2041 gattctggca tggaaaggac tactgcaatt tcgtcttcaa agactgggtt caacttgata
     2101 aaccttttgc tgatttcatt gacaggtact ccacgccccg gatgccctgg catgacattg
     2161 cctctgcagt ccacgggaag gcggctcgtg atgtggcacg tcacttcatc cagcgctgga
     2221 acttcacaaa aattatgaaa tcaaaatatc ggtccctttc ttatcctttt ctgcttccaa
     2281 agtctcaaac aacagcccat gagttgagat atcaagtgcc tgggtctgtc catgctaacg
     2341 tacagttgct ccgctctgct gctgattggt ctgctggtat aaagtaccat gaagagtcca
     2401 tccacgccgc ttacgtccat gtgatagaga acagcaggca ctatatctat atcgaaaacc
     2461 agtttttcat aagctgtgct gatgacaaag ttgtgttcaa caagataggc gatgccattg
     2521 cccagaggat cctgaaagct cacagggaaa accagaaata ccgggtatat gtcgtgatac
     2581 cacttctgcc agggttcgaa ggagacattt caaccggcgg aggaaatgct ctacaggcaa
     2641 tcatgcactt caactacaga accatgtgca gaggagaaaa ttccatcctt ggacagttaa
     2701 aagcagagct tggtaatcag tggataaatt acatatcatt ctgtggtctt agaacacatg
     2761 cagagctcga aggaaaccta gtaactgagc ttatctatgt ccacagcaag ttgttaattg
     2821 ctgatgataa cactgttatt attggctctg ccaacataaa tgaccgcagc atgctgggaa
     2881 agcgtgacag tgaaatggct gtcattgtgc aagatacaga gactgttcct tcagtaatgg
     2941 atggaaaaga gtaccaagct ggccggtttg cccgaggact tcggctacag tgctttaggg
     3001 ttgtccttgg ctatcttgat gacccaagtg aggacattca ggatccagtg agtgacaaat
     3061 tcttcaagga ggtgtgggtt tcaacagcag ctcgaaatgc tacaatttat gacaaggttt
     3121 tccggtgcct tcccaatgat gaagtacaca atttaattca gctgagagac tttataaaca
     3181 agcccgtatt agctaaggaa gatcccattc gagctgagga ggaactgaag aagatccgtg
     3241 gatttttggt gcaattcccc ttttatttct tgtctgaaga aagcctactg ccttctgttg
     3301 ggaccaaaga ggccatagtg cccatggagg tttggactta agagatattc attggcagct
     3361 caaagacttc caccctggag accacactgc acacagtgac ttcctgggga tgtcatagcc
     3421 aaagccaggc ctgacgcatt ctcgtatcca acccaaggac cttttggaat gactggggag
     3481 ggctgcagtc acattgatgt aaggactgta aacatcagca agactttata attccttctg
     3541 cctaacttgt aaaaaggggg ctgcattctt gttggtagca tgtactctgt tgagtaaaac
     3601 acatattcaa attccgtata ccaaaatcca tttcctttgt aacaagaatt taccagtaac
     3661 tgtgatctag gttgccaaaa gttgtctgaa tctccttatt cttctctgat cttcatttat
     3721 gcagccaatg tctagctgga cctgccctca tcttgcagtt catacaggca ctgtttgaga
     3781 gattgtttat tattagatgt tgtaatgctg cttcaagatt cttcatggtt acatgggatg
     3841 cccctgctca tctggtcctg aagtagtaac attcacccaa atgaggatat agtcattatt
     3901 ccttttcagt cactgtacga acacggcaga ctttagccta cacagtagac tgtgttaggc
     3961 acttctggta acattgacac tgtatcttga caccactaag caacaggaag gaataaattc
     4021 caatattgag acagagattt atttcatttt gctcccaaag gcactgacaa catgagtcct
     4081 tgtgattagg cccacctgat caaatgtaaa aacatgcatg ggatatttga ttacgtaaca
     4141 accagctaaa actgagagca cacagccttc ggaaaccccc aaggtgtcga ggaggatcag
     4201 atccagataa gagaagatga ttccttctag tcctgtaaaa tttcctgtca tccttagctg
     4261 ctgggttgat tgaaatttga tctctacatt tcaaaatcaa actagtgaat acagtttgtc
     4321 tttcaagaga atcagaggca tcgtaactcc taccaagtta gtaagtatga gtctaacctt
     4381 gtttgacatt caggtcttct gctacattat cttctttgag aaaaattaat tgattataaa
     4441 atatgtgcta cttcacttac attattaaat atgtattaac gcctctcacc agggcttcca
     4501 gtgaagttac aatgccctag tctgtgaatt agtctggaaa cgtgtttttc cttttcggat
     4561 gttagagtac cctttgataa actaaatttt actaagctga acaactctga cagtctaaag
     4621 agctaatgtg ggttaccaaa aggcctgtac ctgtaaaaca aaatgcaggt gtaatgatta
     4681 tacatgtcta tggattacct ggacatactc tcatttgggt tgttcttcaa agaagcaagc
     4741 agccgatccc tgttttcata aagctaatac ttcagttgga aaaattaaac aggagcacaa
     4801 agtcagggat aggggttagc agaagagaga aatagtgtca catcaagggc aggatctcat
     4861 agctagggaa catttcacaa ataaggtgag attttgtaac caataataaa aatgaatgtt
     4921 tttataagta aataacttat ttttcatatg gctaaagatg gtaaaatgac ttcattctat
     4981 agccattgta aataagaatt tgctattgat gaaagaagtt cagattggca tttgaagtat
     5041 tgagtgtatg ggatctctaa ggatttctta gattttatat ttaaatattt tttaaacctt
     5101 agaggagtca acaaactggc tcttgatttt cagcacccta ctctcatgaa aaaagcctga
     5161 aaggaccctt tcccttataa gtaatttaat ccaatttctc cccattttat agatgaggaa
     5221 actgaggctc agatcagatg agaactcact taaatccact caatgtgtag atggtagagc
     5281 tgggactagc aacattgctg cagcccattg ttggcctctc tcttcacttt atcattgccc
     5341 aagaatgagg atatgcagta aacagaattc aggcaagata cctctaagct gttttgaacc
     5401 ctctgatatt ttgtatttat gtgtttgtct gtctccccct actagaatgt aagctccatg
     5461 gggcagggac ttcactgtat tttgttcata gtgtatcccc agagcctgga ccagtgcttg
     5521 gcacatagga gatggcaata aatgcttgta gaattaataa acaaggtgaa ggagagagct
     5581 aatgaaatga aaatcttagt ctcatataag aagtcttact actgctttct atggcaagtt
     5641 ccttttctat tgcatcttcc caaagacaag atgctcatct gcaaaggggt gataagattt
     5701 acttggaggg attttatgga aaattcagtg caataattgc tttttttttt ttttttgcag
     5761 tagcagttta catagtacag cattctgcaa aaattgcatg aaatgtggac acaagatgta
     5821 ctgtatgact taattacctg tcattctctg acaccattac tacctactag aagttcaaat
     5881 aatttatctt taacattgca atatctgata aactttttaa aatgtaaata attcaaagcc
     5941 ttgaaaatct atcatgaatc tgctcaacat ttcaaagtga ctattttgaa atgcctataa
     6001 atgctatata cctga
//
