LOCUS       NM_014996               6417 bp    mRNA    linear   PRI 09-JUN-2024
DEFINITION  Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 2,
            mRNA.
ACCESSION   NM_014996 XM_011512564 XM_042635
VERSION     NM_014996.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Drissi,I., Fletcher,E., Shaheen,R., Nahorski,M., Alhashem,A.M.,
            Lisgo,S., Fernandez-Jaen,A., Schon,K., Tlili-Graiess,K.,
            Smithson,S.F., Lindsay,S., J Sharpe,H., Alkuraya,F.S. and Woods,G.
  TITLE     Mutations in phospholipase C eta-1 (PLCH1) are associated with
            holoprosencephaly
  JOURNAL   J Med Genet 59 (4), 358-365 (2022)
   PUBMED   33820834
  REMARK    GeneRIF: Mutations in phospholipase C eta-1 (PLCH1) are associated
            with holoprosencephaly.
REFERENCE   2  (bases 1 to 6417)
  AUTHORS   Tavalaee,M. and Nasr-Esfahani,M.H.
  TITLE     Expression profile of PLCzeta, PAWP, and TR-KIT in association with
            fertilization potential, embryo development, and pregnancy outcomes
            in globozoospermic candidates for intra-cytoplasmic sperm injection
            and artificial oocyte activation
  JOURNAL   Andrology 4 (5), 850-856 (2016)
   PUBMED   27089467
  REMARK    GeneRIF: High fertilization (56.06%) and pregnancy (41.7%) rates
            accomplished in this study following ICSI-AOA indicated that
            expression profiles of PLCzeta, PAWP, and TR-KIT were low in
            globozoospermic individuals
REFERENCE   3  (bases 1 to 6417)
  AUTHORS   Popovics,P., Lu,J., Nadia Kamil,L., Morgan,K., Millar,R.P.,
            Schmid,R., Blindauer,C.A. and Stewart,A.J.
  TITLE     A canonical EF-loop directs Ca(2+) -sensitivity in phospholipase
            C-eta2
  JOURNAL   J Cell Biochem 115 (3), 557-565 (2014)
   PUBMED   24123053
  REMARK    GeneRIF: A molecular model of the PLCeta2 EF-hand domain,
            constructed based upon the structure of calmodulin, suggested both
            EF-loops may participate in Ca(2+) -binding.
REFERENCE   4  (bases 1 to 6417)
  AUTHORS   Zhang,Y., Hua,S., Zhang,A., Kong,X., Jiang,C., Deng,D. and
            Wenlong,B.
  TITLE     Association between polymorphisms in COMT, PLCH1, and CYP17A1, and
            non-small-cell lung cancer risk in Chinese nonsmokers
  JOURNAL   Clin Lung Cancer 14 (1), 45-49 (2013)
   PUBMED   22658813
  REMARK    GeneRIF: The PLCH1 rs181696 single-nucleotide polymorphism was
            strongly associated with an increased risk of squamous cell
            carcinoma.
REFERENCE   5  (bases 1 to 6417)
  AUTHORS   Lauc,G., Huffman,J.E., Pucic,M., Zgaga,L., Adamczyk,B., Muzinic,A.,
            Novokmet,M., Polasek,O., Gornik,O., Kristic,J., Keser,T.,
            Vitart,V., Scheijen,B., Uh,H.W., Molokhia,M., Patrick,A.L.,
            McKeigue,P., Kolcic,I., Lukic,I.K., Swann,O., van Leeuwen,F.N.,
            Ruhaak,L.R., Houwing-Duistermaat,J.J., Slagboom,P.E., Beekman,M.,
            de Craen,A.J., Deelder,A.M., Zeng,Q., Wang,W., Hastie,N.D.,
            Gyllensten,U., Wilson,J.F., Wuhrer,M., Wright,A.F., Rudd,P.M.,
            Hayward,C., Aulchenko,Y., Campbell,H. and Rudan,I.
  TITLE     Loci associated with N-glycosylation of human immunoglobulin G show
            pleiotropy with autoimmune diseases and haematological cancers
  JOURNAL   PLoS Genet 9 (1), e1003225 (2013)
   PUBMED   23382691
REFERENCE   6  (bases 1 to 6417)
  AUTHORS   Bailey,S.D., Xie,C., Do,R., Montpetit,A., Diaz,R., Mohan,V.,
            Keavney,B., Yusuf,S., Gerstein,H.C., Engert,J.C. and Anand,S.
  CONSRTM   DREAM investigators
  TITLE     Variation at the NFATC2 locus increases the risk of
            thiazolidinedione-induced edema in the Diabetes REduction
            Assessment with ramipril and rosiglitazone Medication (DREAM) study
  JOURNAL   Diabetes Care 33 (10), 2250-2253 (2010)
   PUBMED   20628086
  REMARK    GeneRIF: Observational study of gene-disease association,
            gene-environment interaction, and pharmacogenomic / toxicogenomic.
            (HuGE Navigator)
REFERENCE   7  (bases 1 to 6417)
  AUTHORS   Talmud,P.J., Drenos,F., Shah,S., Shah,T., Palmen,J., Verzilli,C.,
            Gaunt,T.R., Pallas,J., Lovering,R., Li,K., Casas,J.P., Sofat,R.,
            Kumari,M., Rodriguez,S., Johnson,T., Newhouse,S.J., Dominiczak,A.,
            Samani,N.J., Caulfield,M., Sever,P., Stanton,A., Shields,D.C.,
            Padmanabhan,S., Melander,O., Hastie,C., Delles,C., Ebrahim,S.,
            Marmot,M.G., Smith,G.D., Lawlor,D.A., Munroe,P.B., Day,I.N.,
            Kivimaki,M., Whittaker,J., Humphries,S.E. and Hingorani,A.D.
  CONSRTM   ASCOT investigators; NORDIL investigators; BRIGHT Consortium
  TITLE     Gene-centric association signals for lipids and apolipoproteins
            identified via the HumanCVD BeadChip
  JOURNAL   Am J Hum Genet 85 (5), 628-642 (2009)
   PUBMED   19913121
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   8  (bases 1 to 6417)
  AUTHORS   Hwang,J.I., Oh,Y.S., Shin,K.J., Kim,H., Ryu,S.H. and Suh,P.G.
  TITLE     Molecular cloning and characterization of a novel phospholipase C,
            PLC-eta
  JOURNAL   Biochem J 389 (Pt 1), 181-186 (2005)
   PUBMED   15702972
  REMARK    GeneRIF: The identification of a novel PLC isoform in the brains of
            human and mouse, named PLC-eta, which contains the conserved
            pleckstrin homology domain, X and Y domains for catalytic activity
            and the C2 domain.
REFERENCE   9  (bases 1 to 6417)
  AUTHORS   Stewart,A.J., Mukherjee,J., Roberts,S.J., Lester,D. and
            Farquharson,C.
  TITLE     Identification of a novel class of mammalian
            phosphoinositol-specific phospholipase C enzymes
  JOURNAL   Int J Mol Med 15 (1), 117-121 (2005)
   PUBMED   15583837
  REMARK    GeneRIF: molecular cloning of PLC-eta1 and PLC-eta2
REFERENCE   10 (bases 1 to 6417)
  AUTHORS   Ballif,B.A., Villen,J., Beausoleil,S.A., Schwartz,D. and Gygi,S.P.
  TITLE     Phosphoproteomic analysis of the developing mouse brain
  JOURNAL   Mol Cell Proteomics 3 (11), 1093-1101 (2004)
   PUBMED   15345747
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC104472.14, AC132006.2 and AC012022.13.
            
            On or before Oct 31, 2019 this sequence version replaced
            XM_011512564.2, NM_014996.3.
            
            Summary: PLCH1 is a member of the PLC-eta family of the
            phosphoinositide-specific phospholipase C (PLC) superfamily of
            enzymes that cleave phosphatidylinositol 4,5-bisphosphate
            (PtdIns(4,5)P2) to generate second messengers inositol
            1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) (Hwang et al.,
            2005 [PubMed 15702972]).[supplied by OMIM, Jun 2009].
            
            Transcript Variant: This variant (2) encodes isoform b.
            
            ##Evidence-Data-START##
            CDS exon combination :: BC113950.1 [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMEA1965299,
                                    SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000460012.7/ ENSP00000417502.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-232               AC104472.14        144429-144660
            233-351             AC132006.2         58852-58970         c
            352-498             AC012022.13        13195-13341
            499-742             AC012022.13        15389-15632
            743-872             AC012022.13        23379-23508
            873-1043            AC012022.13        25931-26101
            1044-1137           AC012022.13        41249-41342
            1138-1341           AC012022.13        44455-44658
            1342-1462           AC012022.13        55377-55497
            1463-1634           AC012022.13        59615-59786
            1635-1742           AC012022.13        85569-85676
            1743-1904           AC012022.13        94689-94850
            1905-1976           AC012022.13        104947-105018
            1977-2068           AC012022.13        108779-108870
            2069-2166           AC012022.13        112156-112253
            2167-2346           AC012022.13        115056-115235
            2347-2454           AC012022.13        115325-115432
            2455-2579           AC012022.13        116720-116844
            2580-2664           AC012022.13        118705-118789
            2665-2811           AC012022.13        120767-120913
            2812-2891           AC012022.13        121466-121545
            2892-3246           AC012022.13        123863-124217
            3247-6417           AC012022.13        126522-129692
FEATURES             Location/Qualifiers
     source          1..6417
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q25.31"
     gene            1..6417
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /note="phospholipase C eta 1"
                     /db_xref="GeneID:23007"
                     /db_xref="HGNC:HGNC:29185"
                     /db_xref="MIM:612835"
     exon            1..232
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /inference="alignment:Splign:2.1.0"
     exon            233..351
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /inference="alignment:Splign:2.1.0"
     CDS             273..5330
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /EC_number="3.1.4.11"
                     /note="isoform b is encoded by transcript variant 2;
                     phospholipase C-like 3; phospholipase C-eta1a;
                     phospholipase C-eta1b; phospholipase C-like protein 3;
                     phosphoinositide phospholipase C-eta-1;
                     1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
                     eta-1; PLC-eta-1"
                     /codon_start=1
                     /product="1-phosphatidylinositol 4,5-bisphosphate
                     phosphodiesterase eta-1 isoform b"
                     /protein_id="NP_055811.2"
                     /db_xref="CCDS:CCDS33881.2"
                     /db_xref="GeneID:23007"
                     /db_xref="HGNC:HGNC:29185"
                     /db_xref="MIM:612835"
                     /translation="MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLK
                     RGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFD
                     PSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVK
                     QTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCV
                     FYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVS
                     EENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQ
                     LLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHA
                     FVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGK
                     ILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLE
                     SLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGK
                     HKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVA
                     AQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPL
                     PYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPA
                     NPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNG
                     FNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGL
                     TEASIFVHITINEIYGKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTA
                     SAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGA
                     LSLPVSETAKDIEGKENSLAEDKDGRRKGKASIKDPHFLNFNKKLSSSSSALLHKDTS
                     QGDTIVSTAHMSVTGEQLGMSSPRGGRTTSNATSNCQENPCPSKSLSPKQHLAPDPVV
                     NPTQDLHGVKIKEKGNPEDFVEGKSILSGSVLSHSNLEIKNLEGNRGKGRAATSFSLS
                     DVSMLCSDIPDLHSTAILQESVISHLIDNVTLTNENEPGSSISALIGQFDETNNQALT
                     VVSHLHNTSVMSGHCPLPSLGLKMPIKHGFCKGKSKSSFLCSSPELIALSSSETTKHA
                     TNTVYETTCTPISKTKPDDDLSSKAKTAALESNLPGSPNTSRGWLPKSPTKGEDWETL
                     KSCSPASSPDLTLEDVIADPTLCFNSGESSLVEIDGESENLSLTTCEYRREGTSQLAS
                     PLKLKYNQGVVEHFQRGLRNGYCKETLRPSVPEIFNNIQDVKTQSISYLAYQGAGFVH
                     NHFSDSDAKMFQTCVPQQSSAQDMHVPVPKQLAHLPLPALKLPSPCKSKSLGDLTSED
                     IACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQEDNCQV
                     LYSKQDANQLPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKVPNPSNGAGVVLRNK
                     PSAPTPAVNRHSTGSYIAGYLKNTKGGGLEGRGIPEGACTALHYGHVDQFCSDNSVLQ
                     TEPSSDDKPEIYFLLRL"
     exon            352..498
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /inference="alignment:Splign:2.1.0"
     exon            499..742
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /inference="alignment:Splign:2.1.0"
     exon            743..872
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /inference="alignment:Splign:2.1.0"
     exon            873..1043
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /inference="alignment:Splign:2.1.0"
     exon            1044..1137
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1341
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /inference="alignment:Splign:2.1.0"
     exon            1342..1462
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /inference="alignment:Splign:2.1.0"
     exon            1463..1634
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /inference="alignment:Splign:2.1.0"
     exon            1635..1742
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /inference="alignment:Splign:2.1.0"
     exon            1743..1904
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /inference="alignment:Splign:2.1.0"
     exon            1905..1976
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /inference="alignment:Splign:2.1.0"
     exon            1977..2068
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2069..2166
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2167..2346
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2347..2454
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2455..2579
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2580..2664
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2665..2811
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2812..2891
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2892..3246
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /inference="alignment:Splign:2.1.0"
     exon            3247..6417
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6397..6402
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /note="hexamer: ATTAAA"
     polyA_site      6417
                     /gene="PLCH1"
                     /gene_synonym="HPE14; PLC eta 1; PLC-L3; PLCL3"
                     /note="major polyA site"
ORIGIN      
        1 gaatgagaaa gcaggttgtg gcggcggcgc cctgctcttc cccgctcgtc tggggcttct
       61 gctggctagt ggggagaagt gctcttctag gggggctttt ctggcccttg gcaggggttt
      121 ggagttggag tcacaggggc caataactca tcaccccagc acttcggagg tggctccttg
      181 tgccacggac tccgaggagt ccttcagcta ctgaacactc aaagctgagg agggcaattt
      241 ggttttgatg ctgtgccaga aatcttctga caatgagtta ctggaacttg gaaaaaagga
      301 actgtgtgca gtaccgcagg cattttctgg tggacaacag tgtgtttcat gttgaaagat
      361 gcatgagtgt gatgcagtcc gggacacaga tgatcaagct gaaacgtgga accaaagggc
      421 ttgtccgcct cttttacctg gatgagcacc ggacacgcct ccgatggcga ccctctagga
      481 agagtgagaa ggcaaaaata cttattgatt ccatttacaa agtgactgag ggccggcagt
      541 ctgaaatatt ccacagacaa gctgagggga acttcgaccc cagctgctgc ttcaccatct
      601 accatggcaa ccacatggag tccctggacc tcatcacctc caaccccgag gaggcccgca
      661 cctggatcac aggcctcaag tacctgatgg ctggcatcag tgatgaagac tcccttgcca
      721 aaaggcagag gacccatgac caatgggtga agcagacctt tgaggaagct gataagaatg
      781 gtgacggctt gctgaatatt gaagagatac atcagctgat gcataaactg aatgttaatc
      841 tgccccgaag aaaagtcaga caaatgtttc aggaagccga cacagatgag aatcagggaa
      901 ctttgacatt tgaagagttc tgtgtttttt acaaaatgat gtctttgaga cgagaccttt
      961 atttgttact tttgagctac agtgacaaga aagatcacct aactgtggaa gaactggctc
     1021 agtttttgaa ggtggagcaa aagatgaata atgtgacaac ggactattgt cttgacatca
     1081 taaagaagtt tgaagtttca gaagaaaata aggtgaaaaa tgttcttggc atagaaggct
     1141 tcacgaactt catgcgtagt cctgcctgtg acatatttaa cccattgcac catgaagtgt
     1201 accaagacat ggatcagccc ctctgcaact actacattgc ttcctctcac aatacatacc
     1261 tgactggaga ccagctcctt tctcagtcca aagtggatat gtatgcacgg gtgctgcaag
     1321 agggctgtcg ctgtgtggaa gttgactgtt gggatggccc agatggagag ccagtagtac
     1381 atcatggtta cactctcact tcaaaaattc tcttcagaga tgttgtggag accatcaaca
     1441 agcatgcctt tgtgaagaat gagtttcctg ttatattgtc tatcgagaat cactgcagta
     1501 tccagcagca aaggaagatt gctcagtacc tgaaaggaat attcggagac aaactggacc
     1561 tgtcatctgt tgatacaggg gagtgcaagc agcttccaag ccctcaaagt ttgaaaggca
     1621 aaattctagt gaagggtaag aagttgcctt atcaccttgg ggatgatgca gaggaagggg
     1681 aagtttctga tgaggacagt gcagatgaaa ttgaagacga gtgcaaattc aagctccatt
     1741 atagtaatgg gaccactgag catcaggtgg aatctttcat aaggaaaaaa ctggagtcac
     1801 tgttaaaaga atctcaaatt cgagataaag aagatcctga tagtttcaca gtgcgggcac
     1861 tactgaaggc cacgcatgaa ggcttaaatg cacacctgaa gcagagtcca gatgtaaagg
     1921 aaagtggaaa gaaatcacat ggacgatccc tcatgaccaa ctttggaaaa cataagaaaa
     1981 ctacaaaatc acggtctaaa tcttacagta ctgatgatga ggaagacaca cagcagagta
     2041 ctggcaagga gggtggccag ctgtacagat tgggtcgccg aaggaaaacc atgaagctct
     2101 gccgagaact ctctgatttg gttgtgtaca caaactccgt ggccgctcag gacattgtgg
     2161 atgacggaac cacaggaaat gtgttatcat tcagtgaaac aagagcacat caggttgttc
     2221 agcaaaaatc agagcagttc atgatttata atcaaaagca actcacgagg atttacccct
     2281 ctgcctaccg cattgattcc agtaacttca accctctccc ctactggaac gcaggctgcc
     2341 agctagtggc actgaattat caatctgaag gacgaatgat gcagttaaac cgagccaaat
     2401 tcaaggcaaa tggcaattgt ggctatgtcc tcaaacccca gcaaatgtgc aaaggtactt
     2461 tcaacccttt ctctggtgac cctcttcctg ccaaccccaa aaagcagctc atcctgaaag
     2521 ttatcagtgg acagcaactc cccaaacctc cagactccat gtttggagat cgaggcgaga
     2581 tcattgaccc ttttgttgaa gttgaaatta ttggattgcc agtagattgt tgtaaagatc
     2641 aaacccgtgt ggtagatgac aatggattta accctgtgtg ggaagaaaca ctgacattta
     2701 cagtacacat gccagaaata gctttggttc ggttccttgt gtgggatcac gatcccattg
     2761 gacgagactt tgttggacaa agaactgtga ccttcagcag cttagtgcct ggctaccggc
     2821 atgtctattt ggaaggactg acagaagcat ccatatttgt acacataacc atcaatgaaa
     2881 tctatggaaa gaacagacaa ctccagggtc tgaagggact gttcaataag aatcctaggc
     2941 acagttcttc agaaaacaat tcccattatg tacggaagcg atccattgga gatagaattc
     3001 tgcgacgcac agctagcgcc ccagccaaag gcaggaaaaa gagcaaaatg ggcttccaag
     3061 aaatggtgga gataaaggat tctgtgtccg aggccacaag agatcaagat ggcgtgctga
     3121 ggaggaccac acgcagtttg caagcacgcc ctgtctctat gcctgttgac agaaaccttc
     3181 tgggagcttt gtcgctgcct gtatctgaaa cagcaaaaga cattgaagga aaagaaaact
     3241 ctctagcaga agataaagat ggcagaagaa aagggaaagc aagtataaaa gatccacatt
     3301 ttctaaattt caacaaaaag ttatcatcct cctccagtgc tctgctccac aaagatacca
     3361 gccaagggga caccattgta tctactgccc acatgtcagt cacaggagaa cagctgggca
     3421 tgtcaagtcc taggggtggg agaaccacat caaatgccac aagcaattgc caggaaaacc
     3481 cctgtcccag caagtctctc tccccaaagc agcatttggc tcccgatcct gtagttaacc
     3541 ccacacaaga tctgcatggt gtgaaaatca aggaaaaggg taatcctgag gactttgtgg
     3601 aagggaaaag catcttgtca ggaagcgtcc tttctcatag caacctagaa attaagaacc
     3661 tggaaggtaa taggggtaag ggccgagctg caacatcctt ttctttgtca gacgtctcca
     3721 tgctctgttc tgacatacct gacctacatt caactgcaat tctgcaggag agtgtaattt
     3781 cccatcttat tgacaatgtc actttaacaa atgagaatga gccgggcagt tccatctcag
     3841 ccctgattgg ccagtttgat gagaccaaca atcaggctct cacagttgtt tctcatcttc
     3901 ataataccag tgtgatgtca ggccattgtc ccttgcctag cctgggccta aaaatgccca
     3961 tcaagcatgg tttttgcaag ggaaaatcca agtcttcctt cctgtgctca tctccggagc
     4021 tgatagcact ctcgagttct gagaccacca aacatgcaac gaacacagtt tatgaaacta
     4081 cctgcactcc catctctaaa accaaaccag atgatgacct ttctagtaag gccaagacag
     4141 cggccttaga aagcaacctg cctggatccc ctaatacttc tcgtggctgg ttaccaaaaa
     4201 gtcctaccaa gggagaagac tgggaaacac tgaagagctg cagccctgcc tcttcccctg
     4261 atttgaccct ggaggatgta atagctgatc ccactctctg tttcaattct ggggagagca
     4321 gccttgtgga aattgatgga gaatcagaaa atctttctct aacaacctgt gaatatagga
     4381 gagagggcac aagtcaactt gcttctcctt taaaactcaa gtacaatcag ggtgtggtag
     4441 aacactttca aagaggtttg agaaacggct actgtaaaga gaccctccgc ccttctgtcc
     4501 ctgaaatatt caacaatatt caagatgtca aaactcaaag tatttcttat ctagcctatc
     4561 agggtgctgg ctttgtgcat aatcatttct cagattcaga tgcaaaaatg ttccagacct
     4621 gtgtgcccca gcaatctagt gctcaagata tgcatgtccc tgtacccaag cagttggcac
     4681 atcttccttt gcctgctctg aaactgccta gtccttgcaa atccaaaagt ctgggggact
     4741 taacatcaga ggacattgcc tgcaattttg agagcaagta ccagtgtatt agtaagagtt
     4801 ttgttacaac tggcattaga gacaagaagg gcgtgactgt gaagacaaag tcgttagagc
     4861 ctatagatgc cctgaccgag cagcttcgga agcttgtgtc ctttgaccag gaagacaact
     4921 gccaagtgct atattcaaag caggatgcca atcagctccc ccgggcactg gtcaggaagt
     4981 tgtcatccag aagtcagagc agagtgcgca atattgctag tcgtgccaag gagaaacagg
     5041 aagccaacaa gcagaaagtt ccaaacccca gcaatggggc aggagtggtt cttagaaaca
     5101 aaccctcagc acccacccct gcagtgaatc gccactccac cggctcctac atcgcaggct
     5161 acctgaagaa cacgaaaggg ggtggccttg aaggccgggg catcccagag ggggcatgca
     5221 cggctcttca ctatggccac gttgaccagt tttgttcaga taattctgtt ttgcagactg
     5281 agccaagcag tgatgataaa ccagaaattt attttctttt gagactgtga attatttaaa
     5341 actgcattct taaggttttc aaggtattct gtagaatgtc agagttttca gtaaataagt
     5401 tccttggctt tggaatgatt ttcaaatgta tttttaaact tgtatttggg tctccctttg
     5461 acttcatgtg ttctccctgt taatgcgttt gtatatagat ttggtgacat ttcccatgta
     5521 cctattatga tctttctccc ttgagttgtt gtaaactgta tcagtatgaa atatgtgcat
     5581 gccctagatg tgaaactcct cagcagctcg ggttataatg tgtgtgtgtt tcaccaagat
     5641 gtcctttact gtgtcctcag attgcagtca caaatgtagt catttttact ctctaactgt
     5701 tgagttgatt aagagacttt gcaaacgaca aagggatgaa ttccttactt taatctgtta
     5761 tcatttttcc tatgtttccc tctttgttca gaagcccaga tgcattttta taactcagtt
     5821 ttaaaaactt taaaatagtt accttgcctt ttaggatgtt cttatcccac ccataatgag
     5881 agttgaaagg ggatggatag ctgctcccca tgcccttccc actttttgga ataggccgtg
     5941 agggtgtgag gaagaaggct gtcttttgta cataaggaca aaattgtttg ttttacataa
     6001 attttgttac atatttttgc taatggcttt gtatgtaaca agaagcgagt tgccaaacta
     6061 cctgttgtac ttttgaattt tctgattgaa ttacagactg cgaacaacgg ctttcagaat
     6121 gagggacttc catcagactc taatgataat agtagcacaa attgaaaact tccccaaagc
     6181 tttcacagaa tattttctca taataaaatc caagtgaaca gataattaga agaaaccctt
     6241 ttccttcagg gaaccaagca actctatttt agtactgaca tgcattattt tcactgtgaa
     6301 ttcacttttt tattgcatgt tcagatgtcc ctctttgttt tttttttttg taacattaac
     6361 tgcaatgatg ttcttcctgg aattcatgaa aatataatta aaacacattt ttaaaca
//
