LOCUS       NM_002661               8666 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens phospholipase C gamma 2 (PLCG2), transcript variant 1,
            mRNA.
ACCESSION   NM_002661 XM_005255985
VERSION     NM_002661.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8666)
  AUTHORS   Bull,D., Matte,J.C., Navarron,C.M., McIntyre,R., Whiting,P.,
            Katan,M., Ducotterd,F. and Magno,L.
  TITLE     The hypermorphic PLCgamma2 S707Y variant dysregulates microglial
            cell function - Insight into PLCgamma2 activation in brain health
            and disease, and opportunities for therapeutic modulation
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1870 (2), 166978 (2024)
   PUBMED   38061598
  REMARK    GeneRIF: The hypermorphic PLCgamma2 S707Y variant dysregulates
            microglial cell function - Insight into PLCgamma2 activation in
            brain health and disease, and opportunities for therapeutic
            modulation.
REFERENCE   2  (bases 1 to 8666)
  AUTHORS   Baysac,K., Sun,G., Nakano,H., Schmitz,E.G., Cruz,A.C., Fisher,C.,
            Bailey,A.C., Mace,E., Milner,J.D. and Ombrello,M.J.
  CONSRTM   PLCG2-Immune Dysregulation Working Group
  TITLE     PLCG2-associated immune dysregulation (PLAID) comprises broad and
            distinct clinical presentations related to functional classes of
            genetic variants
  JOURNAL   J Allergy Clin Immunol 153 (1), 230-242 (2024)
   PUBMED   37769878
  REMARK    GeneRIF: PLCG2-associated immune dysregulation (PLAID) comprises
            broad and distinct clinical presentations related to functional
            classes of genetic variants.
REFERENCE   3  (bases 1 to 8666)
  AUTHORS   Alinger,J.B., Mace,E.M., Porter,J.R., Mah-Som,A.Y., Daugherty,A.L.,
            Li,S., Throm,A.A., Pingel,J.T., Saucier,N., Yao,A., Chinn,I.K.,
            Lupski,J.R., Ehlayel,M., Keller,M., Bowman,G.R., Cooper,M.A.,
            Orange,J.S. and French,A.R.
  TITLE     Human PLCG2 haploinsufficiency results in a novel natural killer
            cell immunodeficiency
  JOURNAL   J Allergy Clin Immunol 153 (1), 216-229 (2024)
   PUBMED   37714437
  REMARK    GeneRIF: Human PLCG2 haploinsufficiency results in a novel natural
            killer cell immunodeficiency.
REFERENCE   4  (bases 1 to 8666)
  AUTHORS   Obata,Y., Kurokawa,K., Tojima,T., Natsume,M., Shiina,I.,
            Takahashi,T., Abe,R., Nakano,A. and Nishida,T.
  TITLE     Golgi retention and oncogenic KIT signaling via
            PLCgamma2-PKD2-PI4KIIIbeta activation in gastrointestinal stromal
            tumor cells
  JOURNAL   Cell Rep 42 (9), 113035 (2023)
   PUBMED   37616163
  REMARK    GeneRIF: Golgi retention and oncogenic KIT signaling via
            PLCgamma2-PKD2-PI4KIIIbeta activation in gastrointestinal stromal
            tumor cells.
REFERENCE   5  (bases 1 to 8666)
  AUTHORS   Kang,J.S., Kohlhuber,F., Hug,H., Marme,D., Eick,D. and Ueffing,M.
  TITLE     Cloning and functional analysis of the hematopoietic cell-specific
            phospholipase C(gamma)2 promoter
  JOURNAL   FEBS Lett 399 (1-2), 14-20 (1996)
   PUBMED   8980110
REFERENCE   6  (bases 1 to 8666)
  AUTHORS   Zauli,G., Previati,M., Caramelli,E., Bassini,A., Falcieri,E.,
            Gibellini,D., Bertolaso,L., Bosco,D., Robuffo,I. and Capitani,S.
  TITLE     Exogenous human immunodeficiency virus type-1 Tat protein
            selectively stimulates a phosphatidylinositol-specific
            phospholipase C nuclear pathway in the Jurkat T cell line
  JOURNAL   Eur J Immunol 25 (9), 2695-2700 (1995)
   PUBMED   7589147
REFERENCE   7  (bases 1 to 8666)
  AUTHORS   Argeson,A.C., Druck,T., Veronese,M.L., Knopf,J.L., Buchberg,A.M.,
            Huebner,K. and Siracusa,L.D.
  TITLE     Phospholipase C gamma-2 (Plcg2) and phospholipase C gamma-1 (Plcg1)
            map to distinct regions in the human and mouse genomes
  JOURNAL   Genomics 25 (1), 29-35 (1995)
   PUBMED   7774933
REFERENCE   8  (bases 1 to 8666)
  AUTHORS   Liao,F., Shin,H.S. and Rhee,S.G.
  TITLE     In vitro tyrosine phosphorylation of PLC-gamma 1 and PLC-gamma 2 by
            src-family protein tyrosine kinases
  JOURNAL   Biochem Biophys Res Commun 191 (3), 1028-1033 (1993)
   PUBMED   7682059
REFERENCE   9  (bases 1 to 8666)
  AUTHORS   Ohta,S., Matsui,A., Nazawa,Y. and Kagawa,Y.
  TITLE     Complete cDNA encoding a putative phospholipase C from transformed
            human lymphocytes
  JOURNAL   FEBS Lett 242 (1), 31-35 (1988)
   PUBMED   2849563
REFERENCE   10 (bases 1 to 8666)
  AUTHORS   Banno,Y., Yada,Y. and Nozawa,Y.
  TITLE     Purification and characterization of membrane-bound phospholipase C
            specific for phosphoinositides from human platelets
  JOURNAL   J Biol Chem 263 (23), 11459-11465 (1988)
   PUBMED   2841328
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB456860.1, BP351485.1,
            BX504887.1, AB208914.1, BC007565.1 and AC092142.5.
            
            On Nov 22, 2018 this sequence version replaced NM_002661.4.
            
            Summary: The protein encoded by this gene is a transmembrane
            signaling enzyme that catalyzes the conversion of
            1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol
            1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as
            a cofactor. IP3 and DAG are second messenger molecules important
            for transmitting signals from growth factor receptors and immune
            system receptors across the cell membrane. Mutations in this gene
            have been found in autoinflammation, antibody deficiency, and
            immune dysregulation syndrome and familial cold autoinflammatory
            syndrome 3. [provided by RefSeq, Mar 2014].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB208914.1, SRR1803616.11578.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000564138.6/ ENSP00000482457.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 DB456860.1         34-36
            4-354               BP351485.1         1-351
            355-355             BX504887.1         155-155
            356-532             BP351485.1         353-529
            533-1329            AB208914.1         522-1318
            1330-1368           BC007565.1         1296-1334
            1369-1677           AB208914.1         1358-1666
            1678-2827           BC007565.1         1644-2793
            2828-3837           AB208914.1         2817-3826
            3838-4275           BC007565.1         3804-4241
            4276-8666           AC092142.5         136076-140466       c
FEATURES             Location/Qualifiers
     source          1..8666
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q23.3"
     gene            1..8666
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /note="phospholipase C gamma 2"
                     /db_xref="GeneID:5336"
                     /db_xref="HGNC:HGNC:9066"
                     /db_xref="MIM:600220"
     exon            1..134
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            135..374
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     CDS             182..3979
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /EC_number="3.1.4.11"
                     /note="phospholipase C-IV; phosphoinositide phospholipase
                     C-gamma-2; phospholipase C, gamma 2
                     (phosphatidylinositol-specific)"
                     /codon_start=1
                     /product="1-phosphatidylinositol 4,5-bisphosphate
                     phosphodiesterase gamma-2"
                     /protein_id="NP_002652.2"
                     /db_xref="CCDS:CCDS42204.1"
                     /db_xref="GeneID:5336"
                     /db_xref="HGNC:HGNC:9066"
                     /db_xref="MIM:600220"
                     /translation="MSTTVNVDSLAEYEKSQIKRALELGTVMTVFSFRKSTPERRTVQ
                     VIMETRQVAWSKTADKIEGFLDIMEIKEIRPGKNSKDFERAKAVRQKEDCCFTILYGT
                     QFVLSTLSLAADSKEDAVNWLSGLKILHQEAMNASTPTIIESWLRKQIYSVDQTRRNS
                     ISLRELKTILPLINFKVSSAKFLKDKFVEIGAHKDELSFEQFHLFYKKLMFEQQKSIL
                     DEFKKDSSVFILGNTDRPDASAVYLHDFQRFLIHEQQEHWAQDLNKVRERMTKFIDDT
                     MRETAEPFLFVDEFLTYLFSRENSIWDEKYDAVDMQDMNNPLSHYWISSSHNTYLTGD
                     QLRSESSPEAYIRCLRMGCRCIELDCWDGPDGKPVIYHGWTRTTKIKFDDVVQAIKDH
                     AFVTSSFPVILSIEEHCSVEQQRHMAKAFKEVFGDLLLTKPTEASADQLPSPSQLREK
                     IIIKHKKLGPRGDVDVNMEDKKDEHKQQGELYMWDSIDQKWTRHYCAIADAKLSFSDD
                     IEQTMEEEVPQDIPPTELHFGEKWFHKKVEKRTSAEKLLQEYCMETGGKDGTFLVRES
                     ETFPNDYTLSFWRSGRVQHCRIRSTMEGGTLKYYLTDNLTFSSIYALIQHYRETHLRC
                     AEFELRLTDPVPNPNPHESKPWYYDSLSRGEAEDMLMRIPRDGAFLIRKREGSDSYAI
                     TFRARGKVKHCRINRDGRHFVLGTSAYFESLVELVSYYEKHSLYRKMRLRYPVTPELL
                     ERYNMERDINSLYDVSRMYVDPSEINPSMPQRTVKALYDYKAKRSDELSFCRGALIHN
                     VSKEPGGWWKGDYGTRIQQYFPSNYVEDISTADFEELEKQIIEDNPLGSLCRGILDLN
                     TYNVVKAPQGKNQKSFVFILEPKQQGDPPVEFATDRVEELFEWFQSIREITWKIDTKE
                     NNMKYWEKNQSIAIELSDLVVYCKPTSKTKDNLENPDFREIRSFVETKADSIIRQKPV
                     DLLKYNQKGLTRVYPKGQRVDSSNYDPFRLWLCGSQMVALNFQTADKYMQMNHALFSL
                     NGRTGYVLQPESMRTEKYDPMPPESQRKILMTLTVKVLGARHLPKLGRSIACPFVEVE
                     ICGAEYDNNKFKTTVVNDNGLSPIWAPTQEKVTFEIYDPNLAFLRFVVYEEDMFSDPN
                     FLAHATYPIKAVKSGFRSVPLKNGYSEDIELASLLVFCEMRPVLESEEELYSSCRQLR
                     RRQEELNNQLFLYDTHQNLRNANRDALVKEFSVNENQLQLYQEKCNKRLREKRVSNSK
                     FYS"
     exon            375..518
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            519..612
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            613..660
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            661..745
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            746..829
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            830..873
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            874..946
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1048
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            1049..1167
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            1168..1253
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            1254..1374
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            1375..1543
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            1544..1648
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            1649..1738
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            1739..1914
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            1915..2115
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            2116..2235
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            2236..2416
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            2417..2488
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            2489..2598
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            2599..2695
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            2696..2762
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            2763..2920
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            2921..3023
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            3024..3233
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            3234..3379
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            3380..3494
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            3495..3662
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            3663..3751
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            3752..3936
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     exon            3937..8666
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4253..4258
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /note="hexamer: AATAAA"
     polyA_site      4275
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /note="major polyA site"
     regulatory      8644..8649
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
                     /note="hexamer: AATAAA"
     polyA_site      8666
                     /gene="PLCG2"
                     /gene_synonym="APLAID; FCAS3; PLC-gamma-2; PLC-IV"
ORIGIN      
        1 agaagtagcg agcgccggcg gcggagggcg tgagcggcgc tgagtgaccc gagtcgggac
       61 gcgggctgcg cgcgcgggac cccggagccc aaacccgggg caggcgggca gctgtgcccg
      121 ggcggcacgg ccagcttcct gatttctccc gattccttcc ttctccctgg agcggccgac
      181 aatgtccacc acggtcaatg tagattccct tgcggaatat gagaagagcc agatcaagag
      241 agccctggag ctggggacgg tgatgactgt gttcagcttc cgcaagtcca cccccgagcg
      301 gagaaccgtc caggtgatca tggagacgcg gcaggtggcc tggagcaaga ccgctgacaa
      361 gatcgagggc ttcttggata tcatggaaat aaaagaaatc cgcccaggga agaactccaa
      421 agatttcgag cgagcaaaag cagttcgcca gaaagaagac tgctgcttca ccatcctata
      481 tggcactcag ttcgtcctca gcacgctcag cttggcagct gactctaaag aggatgcagt
      541 taactggctc tctggcttga aaatcttaca ccaggaagcg atgaatgcgt ccacgcccac
      601 cattatcgag agttggctga gaaagcagat atattctgtg gatcaaacca gaagaaacag
      661 catcagtctc cgagagttga agaccatctt gcccctgatc aactttaaag tgagcagtgc
      721 caagttcctt aaagataagt ttgtggaaat aggagcacac aaagatgagc tcagctttga
      781 acagttccat ctcttctata aaaaacttat gtttgaacag caaaaatcga ttctcgatga
      841 attcaaaaag gattcgtccg tgttcatcct ggggaacact gacaggccgg atgcctctgc
      901 tgtttacctg catgacttcc agaggtttct catacatgaa cagcaggagc attgggctca
      961 ggatctgaac aaagtccgtg agcggatgac aaagttcatt gatgacacca tgcgtgaaac
     1021 tgctgagcct ttcttgtttg tggatgagtt cctcacgtac ctgttttcac gagaaaacag
     1081 catctgggat gagaagtatg acgcggtgga catgcaggac atgaacaacc ccctgtctca
     1141 ttactggatc tcctcgtcac ataacacgta ccttacaggt gaccagctgc ggagcgagtc
     1201 gtccccagaa gcttacatcc gctgcctgcg catgggctgt cgctgcattg aactggactg
     1261 ctgggacggg cccgatggga agccggtcat ctaccatggc tggacgcgga ctaccaagat
     1321 caagtttgac gacgtcgtgc aggccatcaa agaccacgcc tttgttacct cgagcttccc
     1381 agtgatcctg tccatcgagg agcactgcag cgtggagcaa cagcgtcaca tggccaaggc
     1441 cttcaaggaa gtatttggcg acctgctgtt gacgaagccc acggaggcca gtgctgacca
     1501 gctgccctcg cccagccagc tgcgggagaa gatcatcatc aagcataaga agctgggccc
     1561 ccgaggcgat gtggatgtca acatggagga caagaaggac gaacacaagc aacaggggga
     1621 gctgtacatg tgggattcca ttgaccagaa atggactcgg cactactgcg ccattgccga
     1681 tgccaagctg tccttcagtg atgacattga acagactatg gaggaggaag tgccccagga
     1741 tataccccct acagaactac attttgggga gaaatggttc cacaagaagg tggagaagag
     1801 gacgagtgcc gagaagttgc tgcaggaata ctgcatggag acggggggca aggatggcac
     1861 cttcctggtt cgggagagcg agaccttccc caatgactac accctgtcct tctggcggtc
     1921 aggccgggtc cagcactgcc ggatccgctc caccatggag ggcgggaccc tgaaatacta
     1981 cttgactgac aacctcacct tcagcagcat ctatgccctc atccagcact accgcgagac
     2041 gcacctgcgc tgcgccgagt tcgagctgcg gctcacggac cctgtgccca accccaaccc
     2101 ccacgagtcc aagccgtggt actatgacag cctgagccgc ggagaggcag aggacatgct
     2161 gatgaggatt ccccgggacg gggccttcct gatccggaag cgagagggga gcgactccta
     2221 tgccatcacc ttcagggcta ggggcaaggt aaagcattgt cgcatcaacc gggacggccg
     2281 gcactttgtg ctggggacct ccgcctattt tgagagtctg gtggagctcg tcagttacta
     2341 cgagaagcat tcactctacc gaaagatgag actgcgctac cccgtgaccc ccgagctcct
     2401 ggagcgctac aatatggaaa gagatataaa ctccctctac gacgtcagca gaatgtatgt
     2461 ggatcccagt gaaatcaatc cgtccatgcc tcagagaacc gtgaaagctc tgtatgacta
     2521 caaagccaag cgaagcgatg agctgagctt ctgccgtggt gccctcatcc acaatgtctc
     2581 caaggagccc gggggctggt ggaaaggaga ctatggaacc aggatccagc agtacttccc
     2641 atccaactac gtcgaggaca tctcaactgc agacttcgag gagctagaaa agcagattat
     2701 tgaagacaat cccttagggt ctctttgcag aggaatattg gacctcaata cctataacgt
     2761 cgtgaaagcc cctcagggaa aaaaccagaa gtcctttgtc ttcatcctgg agcccaagca
     2821 gcagggcgat cctccggtgg agtttgccac agacagggtg gaggagctct ttgagtggtt
     2881 tcagagcatc cgagagatca cctggaagat tgacaccaag gagaacaaca tgaagtactg
     2941 ggagaagaac cagtccatcg ccatcgagct ctctgacctg gttgtctact gcaaaccaac
     3001 cagcaaaacc aaggacaact tagaaaatcc tgacttccga gaaatccgct cctttgtgga
     3061 gacgaaggct gacagcatca tcagacagaa gcccgtcgac ctcctgaagt acaatcaaaa
     3121 gggcctgacc cgcgtctacc caaagggaca aagagttgac tcttcaaact acgacccctt
     3181 ccgcctctgg ctgtgcggtt ctcagatggt ggcactcaat ttccagacgg cagataagta
     3241 catgcagatg aatcacgcat tgttttctct caatgggcgc acgggctacg ttctgcagcc
     3301 tgagagcatg aggacagaga aatatgaccc gatgccaccc gagtcccaga ggaagatcct
     3361 gatgacgctg acagtcaagg ttctcggtgc tcgccatctc cccaaacttg gacgaagtat
     3421 tgcctgtccc tttgtagaag tggagatctg tggagccgag tatgacaaca acaagttcaa
     3481 gacgacggtt gtgaatgata atggcctcag ccctatctgg gctccaacac aggagaaggt
     3541 gacatttgaa atttatgacc caaacctggc atttctgcgc tttgtggttt atgaagaaga
     3601 tatgttcagc gatcccaact ttcttgctca tgccacttac cccattaaag cagtcaaatc
     3661 aggattcagg tccgttcctc tgaagaatgg gtacagcgag gacatagagc tggcttccct
     3721 cctggttttc tgtgagatgc ggccagtcct ggagagcgaa gaggaacttt actcctcctg
     3781 tcgccagctg aggaggcggc aagaagaact gaacaaccag ctctttctgt atgacacaca
     3841 ccagaacttg cgcaatgcca accgggatgc cctggttaaa gagttcagtg ttaatgagaa
     3901 ccagctccag ctgtaccagg agaaatgcaa caagaggtta agagagaaga gagtcagcaa
     3961 cagcaagttt tactcataga agctggggta tgtgtgtaag ggtattgtgt gtgtgcgcat
     4021 gtgtgtttgc atgtaggaga acgtgcccta ttcacactct gggaagacgc taatctgtga
     4081 catcttttct tcaagcctgc catcaaggac atttcttaag acccaactgg catgagttgg
     4141 ggtaatttcc tattattttc atcttggaca actttcttaa cttatattct ttatagagga
     4201 ttccccaaaa tgtgctcctc atttttggcc tctcatgttc caaacctcat tgaataaaag
     4261 caatgaaaac cttgatcaat taagccttct gttgcacgac ctgtgcagtg aacaggattt
     4321 cttttctggc caagaagatt ctacctctaa tgatccaggt aactgatgtc catggaggat
     4381 gagctggaaa tgtaagaaac tattcatgag attctgaaaa ggattttaac tcaaaggcaa
     4441 atgattccat aagggcccaa agagaagccc tacccacagg cagcctgctc agttcaatgt
     4501 actttaacta ccaccggctg cctgctgcag tccacaagaa aatggctgag tgatgggatc
     4561 tgttcattaa gacaatttct aattaatggt gacagcttgt tttgtgacta gagttactgg
     4621 gatggagggt aggaatcttg gggcctcttt gttttaaaaa gcccatcaga gagaccagag
     4681 ccgtgctgca ggggcaggtt ctcacttgcc cctggctctg ccagctgctg ggaggctctg
     4741 gccccactag tccctcatgg ccctactgaa ctggctggga ggctgctgga atggcccttg
     4801 gtccacagct ctccacaggc aagaggtcaa ctgctgcttg aaagaggtag acaaaagtta
     4861 ggttgatggc gaaatgtctc tgggttaccc agtcttctgg agcagcaagc tgagctttaa
     4921 tgggctaagc attagggtgt tacagaaaat ttcaaatgca gccatctccc ttggggcaga
     4981 tctacctagt tcatgacagt atgtgcggct ggccagggct ttacacctct gcatcttaag
     5041 ttgttaatac ataccaataa tgtaatatgg ctttttaaag gagaggagag tgctgggttg
     5101 ggaagggagg tggttggtag agtcacaact tctcaatgag tgaatttaca gctgatggga
     5161 aaaggagtgt aactgtgaaa aacgatggct gtggtgggga agaacaaacc agcagtaagc
     5221 ctgatgtttg atgtggatgg aactggcccc tagaaaccca tctgaccctc ctcttgttac
     5281 ccgaaatgct gggcttagta tgcatgtact gctgaaaagc agggcagaac aaatcaggct
     5341 ctgaccagaa gatccttctg gtcccttcac tctacaaaaa cttactgatc acctccacat
     5401 gccaaataca gtgccaagat ttgggggtgt ggatgtttaa acaaaaagct gtgggtctca
     5461 tcaatcatct ccatccacaa gctcctaaaa gaaagccatt tacctcgctt gaagccagga
     5521 acacagggaa cagcagtctg gccaaggaag ggctgttatc tggtgctatc actccagtta
     5581 ctcctccaac tgggagctgc tattttattt ggcagtcagc aactgaagaa agaacattcc
     5641 tcttagtggc agatgttcaa agcaactttc aagaaaggct aggtgagaaa ggcactggga
     5701 tgagtgctgc aggcactctg tagccagggc cccattagcc tttggccagg tagccaccag
     5761 aacctattta ttgcacctgg catctccccc aacccctctc agctctgtta ggacttccac
     5821 acagcagagc tcaggtgttg ctgtcattac ctcctttcag ctcctcactt cattctactt
     5881 taaagccaca gtgctaaggc ctgcatcccc tttctgccca aatgggtttt ttgctaccat
     5941 atcaaagaac ctgacatatg gcggcatagg aagcagaagc taagcctctc tccagctgct
     6001 gctgtgtaaa atccatgcgt ggccaaagag aagtcagggg attatgacat aaatggtgct
     6061 gggaagaacc ctctgcctaa aactgtctcc ttctcctggt gctacaaccg gaatccacca
     6121 tgagagagta ctttcttcgg ttctttcctc ctgtccttga cagagtaaca cgttaatctg
     6181 gttcttggtg gtgttaggga ctgattctct caggaaaggc acacatggta tgatggctct
     6241 tcccagagtc tatgtgatgc tacataactt cagtatctag ctgagacatg cttcctacat
     6301 gactgttaaa gcacagccaa tccaggccaa gaagactagt aacaggcaca ttctgaaaga
     6361 tggaagcagc actgatagat caaaaccacc actgcatatg tattacactg tttttgttca
     6421 ccattttcct aagtgtgtta tttagaatat tggttattac aaggaaaaat aaagtgggga
     6481 ggctggttag gccttgtgag tttgggaaac ttaggttata aaaactaaat aaagtttttc
     6541 tactgtgaga ctagatgtgc aggagtgaaa ggtgtagagg gtcttgtttt ccaaattcga
     6601 tctcagaatc tttttgccag aagtgtctca tgggacttat ctatagtgga acacatttga
     6661 agacctactg ctctattaag aaggcagccg gacaacatgt tctaatactt cgtatgcttt
     6721 gtgacctagt taaaatctaa acttaagtcg ccatggccag tggcctttag attaagctag
     6781 ccttacccct gggagtatac cagagctttc caaggaatac acagactcca gtactctcag
     6841 gggagcagtg ttcagagcct catcttcctg ttatattctt ctctaagatt catctgcctg
     6901 agaaaatgcc cttttctcac cttacaaaag aaaatatggc tgtctccacc tctagtctta
     6961 ctgtagagca tgtcccaagg tgtaaaaatt caaaatgtgg atatttggaa agtgaaagac
     7021 ttatcaacag ggcacaaatc tttttgcaaa tggattttcc aagtttttct ggtggttcca
     7081 aattttttgc tttcaacaaa gtgggaggaa cagcctgtag atttctgagt ctcttagcat
     7141 gtaactacaa aggggttgga agaattcagt gattctgcta tcataaagct tccgttccca
     7201 ttgatgtatc tgtgtgaaca aggatcaaca tctccataaa tgaaattgaa aacggaaaat
     7261 agaattgatg atgaactttg gctcaatctt aagatgttat caatctacat agatgaaata
     7321 attgtggaga aaagccctct ttatctcatt aagtgataca tttccaaaga agttttacta
     7381 tgtttaataa tttagtgaaa tttgggctat gtgtttattg attcagctca atccagagga
     7441 aaattttaaa ggcttacagc cttaggatta taggatacta tataatactt ttggtacaga
     7501 gatagaatta aataacataa aaatcaaaaa tttattaggc taaaattttg agggagaagt
     7561 ggtatgaaaa tacaaattca aggagtaaaa ggaaaagtgg ggcattcctt gctactaaaa
     7621 attgccttgt tccaggtaag actgatcata aaaaaatggc cctgttcata aaatttttaa
     7681 aaagatcata gtatctatca aataacttat attaagaacc tcctgggcta aatttaaaaa
     7741 gtaatacaac agttttattt aaacatgtag tgtctacggt atgccagcac tttgcagcta
     7801 tttataatga gaaattttag atgtcaatat agcaatgtgc aagaagatag agattttcaa
     7861 aattcactta agagtatctg agcataaaat gttaagattg ctgatcggat gtgagggcga
     7921 tctggctgcg acatctgtca ccccattgat cgccagggtt gattcggctg atctggctgg
     7981 ctaggtgggt gtccccttcc tacctcaccg ctccatgtgc gtccctcccg aagctgcgcg
     8041 ctccgtcgaa gaggacgacc aaccccgata gaggaggacc ggtcttcggt caagggtata
     8101 cgagtagctg cgctcccctg ctggaacctc caaacaagct ctcaagattg ctgatctagg
     8161 gccactaagt gatgaattgt atttggaagc aaaaaggatg gctaaaaagg acctcaaccc
     8221 ttttgacttt aaaaggaaaa tagcttaacc ttcaacctgt gtgacattta actttttgaa
     8281 cccaaccgta aaagctatct tctaaccaac aaaaagttaa taattagatt tggaattata
     8341 cagaattaga aaattggcat ttaaaaatac tcaataattt gtccctggtt tttaattttc
     8401 aaaatatttt ctttttgaag agccagattc cagtgatcct gcctctcaga aatttccaca
     8461 tttcttattt ttcattaggc cttaagaagc tgcatttgta aacttgtgtt tcattattaa
     8521 agcttaattt attttttata taaatagtat gtgctttgtg tacatagaga attaagtgaa
     8581 tgagtcacac agatgttggc tgttgttaat gtgaaaatta aacagctgta tcacattttg
     8641 aaaaataaaa gtttcatctg aatgaa
//
