LOCUS       NM_002660               7092 bp    mRNA    linear   PRI 12-DEC-2024
DEFINITION  Homo sapiens phospholipase C gamma 1 (PLCG1), transcript variant 1,
            mRNA.
ACCESSION   NM_002660
VERSION     NM_002660.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Tao,P., Han,X., Wang,Q., Wang,S., Zhang,J., Liu,L., Fan,X., Liu,C.,
            Liu,M., Guo,L., Lee,P.Y., Aksentijevich,I. and Zhou,Q.
  TITLE     A gain-of-function variation in PLCG1 causes a new immune
            dysregulation disease
  JOURNAL   J Allergy Clin Immunol 152 (5), 1292-1302 (2023)
   PUBMED   37422272
  REMARK    GeneRIF: A gain-of-function variation in PLCG1 causes a new immune
            dysregulation disease.
REFERENCE   2  (bases 1 to 7092)
  AUTHORS   Sjoberg,E., Melssen,M., Richards,M., Ding,Y., Chanoca,C., Chen,D.,
            Nwadozi,E., Pal,S., Love,D.T., Ninchoji,T., Shibuya,M., Simons,M.,
            Dimberg,A. and Claesson-Welsh,L.
  TITLE     Endothelial VEGFR2-PLCgamma signaling regulates vascular
            permeability and antitumor immunity through eNOS/Src
  JOURNAL   J Clin Invest 133 (20), e161366 (2023)
   PUBMED   37651195
  REMARK    GeneRIF: Endothelial VEGFR2-PLCgamma signaling regulates vascular
            permeability and antitumor immunity through eNOS/Src.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7092)
  AUTHORS   Prawiro,C., Bunney,T.D., Kampyli,C., Yaguchi,H., Katan,M. and
            Bangham,C.R.M.
  TITLE     A frequent PLCgamma1 mutation in adult T-cell leukemia/lymphoma
            determines functional properties of the malignant cells
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1869 (2), 166601 (2023)
   PUBMED   36442790
  REMARK    GeneRIF: A frequent PLCgamma1 mutation in adult T-cell
            leukemia/lymphoma determines functional properties of the malignant
            cells.
REFERENCE   4  (bases 1 to 7092)
  AUTHORS   Seo,E.B., Jang,H.J., Kwon,S.H., Kwon,Y.J., Kim,S.K., Lee,S.H.,
            Jeong,A.J., Shin,H.M., Kim,Y.N., Ma,S., Kim,H., Lee,Y.H., Suh,P.G.
            and Ye,S.K.
  TITLE     Loss of phospholipase Cgamma1 suppresses hepatocellular
            carcinogenesis through blockade of STAT3-mediated cancer
            development
  JOURNAL   Hepatol Commun 6 (11), 3234-3246 (2022)
   PUBMED   36153805
  REMARK    GeneRIF: Loss of phospholipase Cgamma1 suppresses hepatocellular
            carcinogenesis through blockade of STAT3-mediated cancer
            development.
REFERENCE   5  (bases 1 to 7092)
  AUTHORS   Upshaw,J.L., Schoon,R.A., Dick,C.J., Billadeau,D.D. and
            Leibson,P.J.
  TITLE     The isoforms of phospholipase C-gamma are differentially used by
            distinct human NK activating receptors
  JOURNAL   J Immunol 175 (1), 213-218 (2005)
   PUBMED   15972651
  REMARK    GeneRIF: PLC-gamma1 and PLC-gamma2 both regulate the functions of
            ITAM-containing receptors, whereas only PLC-gamma2 regulates the
            function of DAP10-coupled receptors.
REFERENCE   6  (bases 1 to 7092)
  AUTHORS   Ronnstrand,L., Mori,S., Arridsson,A.K., Eriksson,A., Wernstedt,C.,
            Hellman,U., Claesson-Welsh,L. and Heldin,C.H.
  TITLE     Identification of two C-terminal autophosphorylation sites in the
            PDGF beta-receptor: involvement in the interaction with
            phospholipase C-gamma
  JOURNAL   EMBO J 11 (11), 3911-3919 (1992)
   PUBMED   1396585
REFERENCE   7  (bases 1 to 7092)
  AUTHORS   Muller,A.J., Pendergast,A.M., Havlik,M.H., Puil,L., Pawson,T. and
            Witte,O.N.
  TITLE     A limited set of SH2 domains binds BCR through a high-affinity
            phosphotyrosine-independent interaction
  JOURNAL   Mol Cell Biol 12 (11), 5087-5093 (1992)
   PUBMED   1383690
REFERENCE   8  (bases 1 to 7092)
  AUTHORS   Weber,J.R., Bell,G.M., Han,M.Y., Pawson,T. and Imboden,J.B.
  TITLE     Association of the tyrosine kinase LCK with phospholipase C-gamma 1
            after stimulation of the T cell antigen receptor
  JOURNAL   J Exp Med 176 (2), 373-379 (1992)
   PUBMED   1500851
REFERENCE   9  (bases 1 to 7092)
  AUTHORS   Hernandez-Sotomayor,S.M. and Carpenter,G.
  TITLE     Epidermal growth factor receptor: elements of intracellular
            communication
  JOURNAL   J Membr Biol 128 (2), 81-89 (1992)
   PUBMED   1501243
  REMARK    Review article
REFERENCE   10 (bases 1 to 7092)
  AUTHORS   Burgess,W.H., Dionne,C.A., Kaplow,J., Mudd,R., Friesel,R.,
            Zilberstein,A., Schlessinger,J. and Jaye,M.
  TITLE     Characterization and cDNA cloning of phospholipase C-gamma, a major
            substrate for heparin-binding growth factor 1 (acidic fibroblast
            growth factor)-activated tyrosine kinase
  JOURNAL   Mol Cell Biol 10 (9), 4770-4777 (1990)
   PUBMED   2167438
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB210028.1, M34667.1,
            AL110247.1 and AL022394.3.
            
            On Jun 2, 2019 this sequence version replaced NM_002660.2.
            
            Summary: The protein encoded by this gene catalyzes the formation
            of inositol 1,4,5-trisphosphate and diacylglycerol from
            phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium
            as a cofactor and plays an important role in the intracellular
            transduction of receptor-mediated tyrosine kinase activators. For
            example, when activated by SRC, the encoded protein causes the Ras
            guanine nucleotide exchange factor RasGRP1 to translocate to the
            Golgi, where it activates Ras. Also, this protein has been shown to
            be a major substrate for heparin-binding growth factor 1 (acidic
            fibroblast growth factor)-activated tyrosine kinase. Two transcript
            variants encoding different isoforms have been found for this gene.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.56084.1,
                                           SRR1803611.150658.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000685551.1/ ENSP00000508698.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2536              AB210028.1         307-2842
            2537-3744           M34667.1           2514-3721
            3745-3794           AL110247.1         1501-1550
            3795-5183           AB210028.1         4098-5486
            5184-7092           AL022394.3         70894-72802
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q12"
     gene            1..7092
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /note="phospholipase C gamma 1"
                     /db_xref="GeneID:5335"
                     /db_xref="HGNC:HGNC:9065"
                     /db_xref="MIM:172420"
     exon            1..316
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     CDS             100..3975
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /EC_number="3.1.4.11"
                     /note="isoform a is encoded by transcript variant 1;
                     phospholipase C, gamma 1 (formerly subtype 148);
                     triphosphoinositide phosphodiesterase; phosphoinositidase
                     C; monophosphatidylinositol phosphodiesterase;
                     phosphatidylinositol phospholipase C;
                     1-phosphatidyl-D-myo-inositol-4,5-bisphosphate;
                     inositoltrisphosphohydrolase; phosphoinositide
                     phospholipase C; PLC-gamma-1; phospholipase C-148;
                     PLC-148; phospholipase C-II; phosphoinositide
                     phospholipase C-gamma-1; 1-phosphatidylinositol
                     4,5-bisphosphate phosphodiesterase gamma-1"
                     /codon_start=1
                     /product="1-phosphatidylinositol 4,5-bisphosphate
                     phosphodiesterase gamma-1 isoform a"
                     /protein_id="NP_002651.2"
                     /db_xref="CCDS:CCDS13313.1"
                     /db_xref="GeneID:5335"
                     /db_xref="HGNC:HGNC:9065"
                     /db_xref="MIM:172420"
                     /translation="MAGAASPCANGCGPGAPSDAEVLHLCRSLEVGTVMTLFYSKKSQ
                     RPERKTFQVKLETRQITWSRGADKIEGAIDIREIKEIRPGKTSRDFDRYQEDPAFRPD
                     QSHCFVILYGMEFRLKTLSLQATSEDEVNMWIKGLTWLMEDTLQAPTPLQIERWLRKQ
                     FYSVDRNREDRISAKDLKNMLSQVNYRVPNMRFLRERLTDLEQRSGDITYGQFAQLYR
                     SLMYSAQKTMDLPFLEASTLRAGERPELCRVSLPEFQQFLLDYQGELWAVDRLQVQEF
                     MLSFLRDPLREIEEPYFFLDEFVTFLFSKENSVWNSQLDAVCPDTMNNPLSHYWISSS
                     HNTYLTGDQFSSESSLEAYARCLRMGCRCIELDCWDGPDGMPVIYHGHTLTTKIKFSD
                     VLHTIKEHAFVASEYPVILSIEDHCSIAQQRNMAQYFKKVLGDTLLTKPVEISADGLP
                     SPNQLKRKILIKHKKLAEGSAYEEVPTSMMYSENDISNSIKNGILYLEDPVNHEWYPH
                     YFVLTSSKIYYSEETSSDQGNEDEEEPKEVSSSTELHSNEKWFHGKLGAGRDGRHIAE
                     RLLTEYCIETGAPDGSFLVRESETFVGDYTLSFWRNGKVQHCRIHSRQDAGTPKFFLT
                     DNLVFDSLYDLITHYQQVPLRCNEFEMRLSEPVPQTNAHESKEWYHASLTRAQAEHML
                     MRVPRDGAFLVRKRNEPNSYAISFRAEGKIKHCRVQQEGQTVMLGNSEFDSLVDLISY
                     YEKHPLYRKMKLRYPINEEALEKIGTAEPDYGALYEGRNPGFYVEANPMPTFKCAVKA
                     LFDYKAQREDELTFIKSAIIQNVEKQEGGWWRGDYGGKKQLWFPSNYVEEMVNPVALE
                     PEREHLDENSPLGDLLRGVLDVPACQIAIRPEGKNNRLFVFSISMASVAHWSLDVAAD
                     SQEELQDWVKKIREVAQTADARLTEGKIMERRKKIALELSELVVYCRPVPFDEEKIGT
                     ERACYRDMSSFPETKAEKYVNKAKGKKFLQYNRLQLSRIYPKGQRLDSSNYDPLPMWI
                     CGSQLVALNFQTPDKPMQMNQALFMTGRHCGYVLQPSTMRDEAFDPFDKSSLRGLEPC
                     AISIEVLGARHLPKNGRGIVCPFVEIEVAGAEYDSTKQKTEFVVDNGLNPVWPAKPFH
                     FQISNPEFAFLRFVVYEEDMFSDQNFLAQATFPVKGLKTGYRAVPLKNNYSEDLELAS
                     LLIKIDIFPAKQENGDLSPFSGTSLRERGSDASGQLFHGRAREGSFESRYQQPFEDFR
                     ISQEHLADHFDSRERRAPRRTRVNGDNRL"
     exon            317..469
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            470..563
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            564..611
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            612..696
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            697..780
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            781..815
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            816..888
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            889..990
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            991..1109
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1195
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..1316
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            1317..1485
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            1486..1608
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            1609..1710
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            1711..1898
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            1899..2099
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            2100..2219
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            2220..2400
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            2401..2478
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            2479..2582
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            2583..2679
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            2680..2749
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            2750..2907
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            2908..3004
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            3005..3229
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            3230..3378
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            3379..3493
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            3494..3655
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            3656..3744
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            3745..3932
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     exon            3933..7092
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5162..5167
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /note="hexamer: AATAAA"
     polyA_site      5187
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /note="major polyA site"
     regulatory      7074..7079
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
                     /note="hexamer: ATTAAA"
     polyA_site      7092
                     /gene="PLCG1"
                     /gene_synonym="IDAA; NCKAP3; PLC-II; PLC1; PLC148;
                     PLCgamma1"
ORIGIN      
        1 gtctgccgcc tcagcctcag ccccaacctc agccgccgcc gttgcgcttg ctcccgggcg
       61 gtcctggcct gtgccgccgc cgcccccagc gtcggagcca tggcgggcgc cgcgtcccct
      121 tgcgccaacg gctgcgggcc cggcgcgccc tcggacgccg aggtgctgca cctctgccgc
      181 agcctcgagg tgggcaccgt catgactttg ttctactcca agaagtcgca gcgacccgag
      241 cggaagacct tccaggtcaa gctggagacg cgccagatca cgtggagccg gggcgccgac
      301 aagatcgagg gggccattga cattcgtgaa attaaggaga tccgcccagg gaagacctca
      361 cgggactttg atcgctatca agaggaccca gctttccggc cggaccagtc acattgcttt
      421 gtcattctct atggaatgga atttcgcctg aaaacgctga gcctgcaagc cacatctgag
      481 gatgaagtga acatgtggat caagggctta acttggctga tggaggatac attgcaggca
      541 cccacacccc tgcagattga gaggtggctc cggaagcagt tttactcagt ggatcggaat
      601 cgtgaggatc gtatatcagc caaggacctg aagaacatgc tgtcccaggt caactaccgg
      661 gtccccaaca tgcgcttcct ccgagagcgg ctgacggacc tggagcagcg cagcggggac
      721 atcacctacg ggcagtttgc tcagctgtac cgcagcctca tgtacagcgc ccagaagacg
      781 atggacctcc ccttcttgga agccagtact ctgagggctg gggagcggcc ggagctttgc
      841 cgagtgtccc ttcctgagtt ccagcagttc cttcttgact accaggggga gctgtgggct
      901 gttgatcgcc tccaggtgca ggagttcatg ctcagcttcc tccgagaccc cttacgagag
      961 atcgaggagc catacttctt cctggatgag tttgtcacct tcctgttctc caaagagaac
     1021 agtgtgtgga actcgcagct ggatgcagta tgcccggaca ccatgaacaa ccctctttcc
     1081 cactactgga tctcctcctc gcacaacacg tacctgaccg gggaccagtt ctccagtgag
     1141 tcctccttgg aagcctatgc tcgctgcctg cggatgggct gtcgctgcat tgagttggac
     1201 tgctgggacg gcccggatgg gatgccagtt atttaccatg ggcacaccct taccaccaag
     1261 atcaagttct cagatgtcct gcacaccatc aaggagcatg cctttgtggc ctcagagtac
     1321 ccagtcatcc tgtccattga ggaccactgc agcattgccc agcagagaaa catggcccaa
     1381 tacttcaaga aggtgctggg ggacacactc ctcaccaagc ccgtggagat ctctgccgac
     1441 gggctcccct cacccaacca gcttaagagg aagatcctca tcaagcacaa gaagctggct
     1501 gagggcagtg cctacgagga ggtgcctaca tccatgatgt actctgagaa cgacatcagc
     1561 aactctatca agaatggcat cctctacctg gaggaccctg tgaaccacga atggtatccc
     1621 cactactttg ttctgaccag cagcaagatc tactactctg aggagaccag cagtgaccag
     1681 ggcaacgagg atgaggagga gcccaaggag gtcagcagca gcacagagct gcactccaat
     1741 gagaagtggt tccatgggaa gctaggggca gggcgtgacg ggcgtcacat cgctgagcgc
     1801 ctgcttactg agtactgcat cgagaccgga gcccctgacg gctccttcct cgtgcgagag
     1861 agtgagacct tcgtgggcga ctacacgctc tctttctggc ggaacgggaa agtccagcac
     1921 tgccgtatcc actcccggca agatgctggg acccccaagt tcttcttgac agacaacctc
     1981 gtctttgact ccctctatga cctcatcacg cactaccagc aggtgcccct gcgctgtaat
     2041 gagtttgaga tgcgactttc agagcctgtc ccacagacca acgcccacga gagcaaagag
     2101 tggtaccacg cgagcctgac cagagcacag gctgagcaca tgctaatgcg cgtccctcgt
     2161 gatggggcct tcctggtgcg gaagcggaat gaacccaact catatgccat ctctttccgg
     2221 gctgagggca agatcaagca ttgccgtgtc cagcaagagg gccagacagt gatgctaggg
     2281 aactcggagt tcgacagcct tgttgacctc atcagctact atgagaaaca cccgctatac
     2341 cgcaagatga agctgcgcta tcccatcaac gaggaggcac tggagaagat tggcacagct
     2401 gagcctgact acggggccct gtatgaggga cgcaaccctg gcttctatgt agaggcaaac
     2461 cctatgccaa ctttcaagtg tgcagtcaaa gccctctttg actacaaggc ccagagggag
     2521 gacgagctga ccttcatcaa gagcgccatc atccagaatg tggagaagca agagggaggc
     2581 tggtggcgag gggactacgg agggaagaag cagctgtggt tcccatcaaa ctacgtggaa
     2641 gagatggtca accccgtggc cctggagccg gagagggagc acttggacga gaacagcccc
     2701 ctaggggact tgctgcgggg ggtcttggat gtgccggctt gtcagattgc catccgtcct
     2761 gagggcaaga acaaccggct cttcgtcttc tccatcagca tggcgtcggt ggcccactgg
     2821 tccctggatg ttgctgccga ctcacaggag gagctgcagg actgggtgaa aaagatccgt
     2881 gaagtggccc agacagcaga cgccaggctc actgaaggga agataatgga acggaggaag
     2941 aagattgccc tggagctctc tgaacttgtc gtctactgcc ggcctgttcc ctttgatgaa
     3001 gagaagattg gcacagaacg tgcttgctac cgggacatgt catccttccc ggaaaccaag
     3061 gctgagaaat acgtgaacaa ggccaaaggc aagaagttcc ttcagtacaa tcgactgcag
     3121 ctctcccgca tctaccccaa gggccagcga ctggattcct ccaactacga tcctttgccc
     3181 atgtggatct gtggcagtca gcttgtggcc ctcaacttcc agacccctga caagcctatg
     3241 cagatgaacc aggccctctt catgacgggc aggcactgtg gctacgtgct gcagccaagc
     3301 accatgcggg atgaggcctt cgaccccttt gacaagagca gcctccgcgg gctggagcca
     3361 tgtgccatct ctattgaggt gctgggggcc cgacatctgc caaagaatgg ccgaggcatt
     3421 gtgtgtcctt ttgtggagat tgaggtggct ggagctgagt atgacagcac caagcagaag
     3481 acagagtttg tggtggacaa tggactcaac cctgtatggc cagccaagcc cttccacttc
     3541 cagatcagta accctgaatt tgcctttctg cgcttcgtgg tgtatgagga agacatgttt
     3601 agtgaccaga atttcctggc tcaggctact ttcccagtaa aaggcctgaa gacaggatac
     3661 agagcagtgc ctttgaagaa caactacagt gaggacctgg agttggcctc cctgctgatc
     3721 aagattgaca ttttccctgc caagcaggag aatggtgacc tcagtccctt cagtggtacg
     3781 tccctgcggg agcggggctc agatgcctca ggccagctgt ttcatggccg agcccgggaa
     3841 ggctcctttg aatcccgcta ccagcagccg tttgaggact tccgcatctc ccaggagcat
     3901 ctcgcagacc attttgacag tcgagaacga agggccccaa gaaggactcg ggtcaatgga
     3961 gacaaccgcc tctagttgta ccccagcctc gttggagagc agcaggtgct gtgcgccttg
     4021 tagaatgccg cgaactgggt tctttggaag cagccccctg tggcggcctt ccgggtctcg
     4081 cagcctgaag cctggattcc agcagtgaat gctagacaga aaccaagcca ttaatgagat
     4141 gttattactg ttttgggcct ccatgcccca gctctggatg aaggcaaaaa ctgtactgtg
     4201 tttcgcatta agcacacaca tctggccctg acttctggag atggatcctt ccatcttgtg
     4261 gggccaggac catggccgaa gccccttgga gagagaggct gcctcagcca gtggcacagg
     4321 agactccaag gagctactga cattcctaag agtggaggag gaggaggagc cttgctgggc
     4381 cagggaaaca aagtttacat tgtcctgtag ctttaaaacc acagctgggc agggtgagaa
     4441 gctagatgcc cctgcagttt ggccctggag ccagggcaga ggaatgtagg gcctgcatgg
     4501 agaagggttc tgccctgcct gaggaggagg acacagcaca agggcacatt gcccatggct
     4561 gggaacatga cccagcctga aagatacagg ggatcatgtt aaaaatagca gtattatttt
     4621 tcgtctcaat ggtattgtaa ctaagttatt tactcctcct gctcctcacc cctgtaggga
     4681 aaccttggag aggagagtgg caggtgggct gcctgctgtg ttaagaggac ttagtttgtg
     4741 atgtaaggca ctgtcaggaa tggggggcgg gccagggtgg gaagagaaga aatagcagag
     4801 cctattttgg tgaggttttt tgtttttaag tcaaagaaga ctcagtatgc tttccctgag
     4861 gaatgaaaaa gggattgagg agttgcctga ctcctgggtg ggtggggtac aggcagttag
     4921 gtgctgaatg aagctgccat ccttgctgca gcttctaact ggtaaaaaga tccagggatg
     4981 gagatgggaa ggttagaaag gcagccctca cctctgagga cagaggccgg ggtccaggcc
     5041 cgtgggcgca aaggtgcctc atagcatagc cagcattcag cacacacaaa cctactgccc
     5101 acatttgggc tcagggttgg ccatttgcta gttctgctgc cctcttaaga tctgactgcc
     5161 aaataaatca tcctcatgtc ctttttcctt tgacttgtat gctctttcgg gggctcagga
     5221 aagcctgttg catgggacat gctcactaga aacagtcgcc agatgattat tctgcagtag
     5281 aagcaggtag gaaatttctg gaaatttctc aggttaagca gcaagagctg taacccctcc
     5341 tctgggctaa caggagttgt gggtccactc tctcctgccc accctctgag ggtgtgtctg
     5401 agcagagtac atcctgcctg ggttctttgt ggccagccag ttttggtggt gagctggaaa
     5461 caaccagagc ccctttccag ttccacagaa acctctcctt tcaaaaatgt tgcattcagt
     5521 tcgttaacac tgccaggtgc cattctgatt gcctctagga cttggcagct gaaatctctg
     5581 ggccctttca acacagttga aaggcccttc tcttcctgaa gctctgtttc catagttggc
     5641 tgtgctggga tggaacaaaa atgacgccac acaaaaaatt taagatagat ccggttccgt
     5701 ggatgacatg aactgatgat agccagtatc aaacagcgat agtgctcagg ttcttgtgtg
     5761 actttctttt acagcttcac agtcccagtt catagagagg agggtggctt tttcccatac
     5821 acaagaaggt ggtgggtggg aattcacctg ggccctcatg atccatgttt cctctctagg
     5881 tttttatggc ctggagagaa aggtttccta tcagagaagg aagaggactg tgtaggccct
     5941 tctgttaggg cccatccacg ttggttagga cgtccttggc gtgtttgtta gtgttgaccc
     6001 ttttagtttt catcaatacg tatctctatt tgctgaacaa gtgtctttct ggaacacaac
     6061 ctggggaaca gacagctctg cctttcttaa ggcagctttg tagacctgag gctcacctct
     6121 cttgggctct gtaagacatt gcctttgcct cactgcaaat gttttgattg ttcttcctag
     6181 tggaaaacgt gccaactctc aagcaaattt aaagatcatt ttcacttcaa gattcctcca
     6241 gacctgacaa atgttgattg acccagaagg cagagtgttt gttggtgggg aagaccctta
     6301 cttggggccg aatgctttgg catcaggagt tgtttgcccc atcccatgca tgcaggccgt
     6361 gtccctacag tgcgacagct cagggttatg acctgtggag tctcaaccct aacagcacat
     6421 tagcaccact tggggagctt ctgaaaaata ctggtacccg gggccttagc ataggtattg
     6481 ttcaaaacct ctcaaggtga tttaaatgag taacaagggt tgaaaaaccc tgattttggc
     6541 agcacaaacc aaaagagcag gcagggccgg gagagggaag tcagtggtac cagaaggtag
     6601 atgggctccc ttgcaggctc cttgttctcc tgccatcacc agtagaacct tctggctgac
     6661 agaccaggga caagtagact gggttcaaag tgacagacct ttcactttca acagctttgg
     6721 ctcagcagac atgtacacat acaaagtaga gcctacaagg tcaggggcat tctgcccccg
     6781 ccacaggact aaagactgcc ctgcgggaag atggcaggag cagtttctga cctcagttga
     6841 gtatctgtgg ccatgagcag aaaaggcagg ggtctgcctc ctgaccaagc acatcttgaa
     6901 catcacctga gagcttgaac atcactaagg acctagacac tcacttgtct ttcaacttga
     6961 gcccatcact caccatgtga gttctgttga gggtgggtag aaagcaaata ggttcaggtt
     7021 atcccaccag actaactcgg tgaatgaaag gatcatgcct tcttcacatt ttaattaaat
     7081 ggatcaagca ca
//
