LOCUS       NM_001377142            5674 bp    mRNA    linear   PRI 11-JUN-2024
DEFINITION  Homo sapiens phospholipase C beta 4 (PLCB4), transcript variant 7,
            mRNA.
ACCESSION   NM_001377142 XM_006723568
VERSION     NM_001377142.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Aparicio-Bautista,D.I., Jimenez-Ortega,R.F., Becerra-Cervera,A.,
            Aquino-Galvez,A., de Leon-Suarez,V.P., Casas-Avila,L., Salmeron,J.,
            Hidalgo-Bravo,A., Rivera-Paredez,B. and Velazquez-Cruz,R.
  TITLE     Interaction between MARK3 (rs11623869), PLCB4 (rs6086746) and
            GEMIN2 (rs2277458) variants with bone mineral density and serum
            25-hidroxivitamin D levels in Mexican Mestizo women
  JOURNAL   Front Endocrinol (Lausanne) 15, 1392063 (2024)
   PUBMED   38715801
  REMARK    GeneRIF: Interaction between MARK3 (rs11623869), PLCB4 (rs6086746)
            and GEMIN2 (rs2277458) variants with bone mineral density and serum
            25-hidroxivitamin D levels in Mexican Mestizo women.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   Kanai,S.M., Heffner,C., Cox,T.C., Cunningham,M.L., Perez,F.A.,
            Bauer,A.M., Reigan,P., Carter,C., Murray,S.A. and Clouthier,D.E.
  TITLE     Auriculocondylar syndrome 2 results from the dominant-negative
            action of PLCB4 variants
  JOURNAL   Dis Model Mech 15 (4) (2022)
   PUBMED   35284927
  REMARK    GeneRIF: Auriculocondylar syndrome 2 results from the
            dominant-negative action of PLCB4 variants.
REFERENCE   3  (bases 1 to 5674)
  AUTHORS   Phan,H.T.N., Kim,N.H., Wei,W., Tall,G.G. and Smrcka,A.V.
  TITLE     Uveal melanoma-associated mutations in PLCbeta4 are constitutively
            activating and promote melanocyte proliferation and tumorigenesis
  JOURNAL   Sci Signal 14 (713), eabj4243 (2021)
   PUBMED   34905385
  REMARK    GeneRIF: Uveal melanoma-associated mutations in PLCbeta4 are
            constitutively activating and promote melanocyte proliferation and
            tumorigenesis.
REFERENCE   4  (bases 1 to 5674)
  AUTHORS   Tsai,D.J., Fang,W.H., Wu,L.W., Tai,M.C., Kao,C.C., Huang,S.M.,
            Chen,W.T., Hsiao,P.J., Chiu,C.C., Su,W., Wu,C.C. and Su,S.L.
  TITLE     The Polymorphism at PLCB4 Promoter (rs6086746) Changes the Binding
            Affinity of RUNX2 and Affects Osteoporosis Susceptibility: An
            Analysis of Bioinformatics-Based Case-Control Study and Functional
            Validation
  JOURNAL   Front Endocrinol (Lausanne) 12, 730686 (2021)
   PUBMED   34899595
  REMARK    GeneRIF: The Polymorphism at PLCB4 Promoter (rs6086746) Changes the
            Binding Affinity of RUNX2 and Affects Osteoporosis Susceptibility:
            An Analysis of Bioinformatics-Based Case-Control Study and
            Functional Validation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5674)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 5674)
  AUTHORS   Lyu,M.S., Park,D.J., Rhee,S.G. and Kozak,C.A.
  TITLE     Genetic mapping of the human and mouse phospholipase C genes
  JOURNAL   Mamm Genome 7 (7), 501-504 (1996)
   PUBMED   8672127
REFERENCE   7  (bases 1 to 5674)
  AUTHORS   Alvarez,R.A., Ghalayini,A.J., Xu,P., Hardcastle,A.,
            Bhattacharya,S., Rao,P.N., Pettenati,M.J., Anderson,R.E. and
            Baehr,W.
  TITLE     cDNA sequence and gene locus of the human retinal
            phosphoinositide-specific phospholipase-C beta 4 (PLCB4)
  JOURNAL   Genomics 29 (1), 53-61 (1995)
   PUBMED   8530101
REFERENCE   8  (bases 1 to 5674)
  AUTHORS   Zauli,G., Previati,M., Caramelli,E., Bassini,A., Falcieri,E.,
            Gibellini,D., Bertolaso,L., Bosco,D., Robuffo,I. and Capitani,S.
  TITLE     Exogenous human immunodeficiency virus type-1 Tat protein
            selectively stimulates a phosphatidylinositol-specific
            phospholipase C nuclear pathway in the Jurkat T cell line
  JOURNAL   Eur J Immunol 25 (9), 2695-2700 (1995)
   PUBMED   7589147
REFERENCE   9  (bases 1 to 5674)
  AUTHORS   Berstein,G., Blank,J.L., Jhon,D.Y., Exton,J.H., Rhee,S.G. and
            Ross,E.M.
  TITLE     Phospholipase C-beta 1 is a GTPase-activating protein for Gq/11,
            its physiologic regulator
  JOURNAL   Cell 70 (3), 411-418 (1992)
   PUBMED   1322796
REFERENCE   10 (bases 1 to 5674)
  AUTHORS   Banno,Y., Yada,Y. and Nozawa,Y.
  TITLE     Purification and characterization of membrane-bound phospholipase C
            specific for phosphoinositides from human platelets
  JOURNAL   J Biol Chem 263 (23), 11459-11465 (1988)
   PUBMED   2841328
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL121898.23, AL121909.10,
            AL023805.2 and AL031652.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Dec 17, 2019 this sequence version replaced XM_006723568.3.
            
            Summary: The protein encoded by this gene catalyzes the formation
            of inositol 1,4,5-trisphosphate and diacylglycerol from
            phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium
            as a cofactor and plays an important role in the intracellular
            transduction of many extracellular signals in the retina. Multiple
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Feb 2010].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074967.2274295.1,
                                           SRR14038192.3542719.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142348, SAMEA2142586
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000378473.9/ ENSP00000367734.5
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-120               AL121898.23        99444-99563
            121-176             AL121898.23        126644-126699
            177-239             AL121909.10        37488-37550
            240-338             AL023805.2         38747-38845
            339-419             AL023805.2         68073-68153
            420-479             AL023805.2         68955-69014
            480-623             AL023805.2         69841-69984
            624-703             AL023805.2         93843-93922
            704-757             AL023805.2         96408-96461
            758-839             AL023805.2         102161-102242
            840-940             AL023805.2         103250-103350
            941-998             AL023805.2         103994-104051
            999-1107            AL023805.2         111001-111109
            1108-1318           AL023805.2         115148-115358
            1319-1412           AL023805.2         118410-118503
            1413-1492           AL023805.2         120826-120905
            1493-1577           AL023805.2         121478-121562
            1578-1668           AL023805.2         124535-124625
            1669-1764           AL023805.2         126470-126565
            1765-1865           AL023805.2         132437-132537
            1866-1901           AL023805.2         136260-136295
            1902-2043           AL031652.4         1185-1326
            2044-2128           AL031652.4         1901-1985
            2129-2253           AL031652.4         2325-2449
            2254-2305           AL031652.4         4305-4356
            2306-2408           AL031652.4         13075-13177
            2409-2573           AL031652.4         14565-14729
            2574-2778           AL031652.4         17016-17220
            2779-2867           AL031652.4         28828-28916
            2868-3018           AL031652.4         30270-30420
            3019-3068           AL031652.4         37249-37298
            3069-3134           AL031652.4         37446-37511
            3135-3250           AL031652.4         46615-46730
            3251-3326           AL031652.4         50682-50757
            3327-3502           AL031652.4         52903-53078
            3503-3604           AL031652.4         61839-61940
            3605-3662           AL031652.4         66058-66115
            3663-3749           AL031652.4         66547-66633
            3750-3786           AL031652.4         69985-70021
            3787-5674           AL031652.4         72189-74076
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p12.3-p12.2"
     gene            1..5674
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /note="phospholipase C beta 4"
                     /db_xref="GeneID:5332"
                     /db_xref="HGNC:HGNC:9059"
                     /db_xref="MIM:600810"
     exon            1..120
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            121..176
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            177..239
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            240..338
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     CDS             255..3875
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /EC_number="3.1.4.11"
                     /note="isoform d is encoded by transcript variant 7;
                     PLC-beta-4; triphosphoinositide phosphodiesterase;
                     phosphoinositidase C; monophosphatidylinositol
                     phosphodiesterase;
                     1-phosphatidyl-D-myo-inositol-4,5-bisphosphate;
                     inositoltrisphosphohydrolase; dJ1119D9.2 (Phopholipase C,
                     beta 4 (1-Phosphatidylinositol-4,5-Bisphosphate
                     Phosphodiesterase Beta 4)); phosphoinositide phospholipase
                     C-beta-4"
                     /codon_start=1
                     /product="1-phosphatidylinositol 4,5-bisphosphate
                     phosphodiesterase beta-4 isoform d"
                     /protein_id="NP_001364071.1"
                     /db_xref="CCDS:CCDS93005.1"
                     /db_xref="GeneID:5332"
                     /db_xref="HGNC:HGNC:9059"
                     /db_xref="MIM:600810"
                     /translation="MAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEF
                     GFFLTWRSEGKEGQVLECSLINSIRSGAIPKDPKILAALEAVGKSENDLEGRIVCVCS
                     GTDLVNISFTYMVAENPEVTKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNT
                     NGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPTAFSYEKFYELTQKICP
                     RTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYE
                     PDEDLKKKGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTG
                     RQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAI
                     KETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRALPS
                     PNDLKRKILIKNKRLKPEVEKKQLEALRSMMEAGESASPANILEDDNEEEIESADQEE
                     EAHPEFKFGNELSADDLGHKEAVANSVKKASDDLEHENNKKGLVTVEDEQAWMASYKY
                     VGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFV
                     NYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGS
                     CGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYG
                     LPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRI
                     LPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSI
                     TEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALA
                     SGVEAKKGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQ
                     VDKIVAQYDKEKSTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVA
                     QHTKEWSEMINTHSAEEQEIRDLHLSQQCELLKKLLINAHEQQTQQLKLSHDRESKEM
                     RAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMD
                     QLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSSMKLQN
                     AN"
     exon            339..419
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            420..479
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            480..623
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            624..703
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            704..757
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            758..839
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            840..940
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            941..998
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            999..1107
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            1108..1318
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            1319..1412
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            1413..1492
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            1493..1577
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            1578..1668
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            1669..1764
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            1765..1865
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            1866..1901
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            1902..2043
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            2044..2128
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            2129..2253
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            2254..2305
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            2306..2408
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            2409..2573
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            2574..2778
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            2779..2867
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            2868..3018
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            3019..3068
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            3069..3134
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            3135..3250
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            3251..3326
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            3327..3502
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            3503..3604
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            3605..3662
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            3663..3749
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            3750..3786
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     exon            3787..5674
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5320..5325
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /note="hexamer: AATAAA"
     polyA_site      5340
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /note="major polyA site"
     regulatory      5645..5650
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
                     /note="hexamer: AATAAA"
     polyA_site      5674
                     /gene="PLCB4"
                     /gene_synonym="ARCND2; ARCND2A; ARCND2B; PI-PLC"
ORIGIN      
        1 acacacagac acacacacgc acacgcacac acgggcgcgc acacacacgc gcgtacacac
       61 tgggtctcca ggcagcggcc gtcgccgcat ccccggcagc agctccaggc aaagtgacag
      121 gaaaagacaa tttctctctg attcagaatc ctgaaaatgt gatctccctt aaaaagagga
      181 cagtgctgct gtgagtttga cgaagtggac atcacctgca gtcagtccag agctgcccag
      241 tcttgaatat aatcatggcc aaaccttatg aatttaactg gcagaaggaa gttccctcct
      301 ttttgcaaga aggagcagtt tttgacagat acgaggagga atcctttgtg tttgaaccca
      361 actgcctctt caaagtggat gagtttggct tctttctgac atggagaagt gaaggcaagg
      421 aaggacaggt gctagaatgc tccctcatca acagtattcg gtcgggagcc ataccaaagg
      481 atcccaaaat cttggctgct cttgaagctg ttggaaaatc agaaaatgat ctggaagggc
      541 ggatagtttg tgtctgcagt ggcacagatc tagtgaacat tagttttacc tacatggtgg
      601 ctgaaaatcc agaagtaact aagcaatggg tagaaggcct gagatcaatc atacacaact
      661 tcagggccaa caacgtcagt ccaatgacat gcctcaagaa acactggatg aaattggcat
      721 ttatgaccaa cacaaatggt aaaattccag ttaggagtat tactagaaca tttgcatcgg
      781 gaaaaacaga aaaggtgatc tttcaagcac tcaaggagtt aggtcttccc agtggaaaga
      841 atgatgaaat tgagcccaca gcattttctt atgaaaagtt ctatgaactg acacaaaaga
      901 tttgtcctcg gacagatata gaagatcttt tcaaaaaaat caatggagac aaaactgatt
      961 atttaacggt agaccaatta gtgagctttc taaatgaaca tcaacgagat cctcgattga
     1021 atgaaatttt atttccattt tatgatgcca aaagggcaat gcagatcatt gagatgtatg
     1081 aacctgatga agatttgaag aaaaaaggcc ttatatcaag tgatgggttt tgcagatatc
     1141 tgatgtcaga tgaaaacgcc ccagtcttcc tagatcgttt agaactttac caagaaatgg
     1201 accatcctct ggctcactac ttcatcagtt cttcccataa cacttatctc actggcagac
     1261 agttcggcgg gaagtcttcg gtagaaatgt acagacaggt tctcctggct ggttgcagat
     1321 gtgttgaact tgactgctgg gatggaaaag gtgaagacca agaaccaata ataactcatg
     1381 gaaaagcaat gtgtacagat atccttttta aggatgtaat tcaagccatc aaggaaactg
     1441 catttgtcac atcagaatat cctgtaattc tctcctttga aaatcactgc agcaaatatc
     1501 aacagtacaa gatgtccaaa tattgcgaag atctatttgg ggatctcctg ttgaaacaag
     1561 cacttgaatc acatccactt gaaccaggca gggctttgcc atcccccaat gacctcaaaa
     1621 gaaaaatact cataaaaaac aagcggctga aacctgaagt tgaaaaaaaa cagctggaag
     1681 ctttgagaag catgatggaa gctggagaat ctgcctcccc agcaaacatc ttagaggacg
     1741 ataatgaaga ggagatcgaa agtgctgacc aagaggagga agctcacccc gaattcaaat
     1801 ttggaaatga actttctgct gatgacttgg gtcacaagga agctgttgca aatagcgtca
     1861 agaaggcttc agatgacctt gaacatgaaa acaacaaaaa gggcctggtc actgtagaag
     1921 atgagcaggc gtggatggca tcttataaat atgtaggtgc taccactaat atccatccat
     1981 atttgtccac aatgatcaac tacgcccagc ctgtaaagtt tcaaggtttc catgtggcag
     2041 aagaacgcaa tattcattat aacatgtctt cttttaatga atcagtcggt cttggctact
     2101 tgaagacaca tgcaattgaa tttgtcaatt ataacaaacg gcaaatgagt cgcatttacc
     2161 ccaagggagg ccgagtcgat tccagtaatt acatgcctca gattttctgg aacgctggct
     2221 gccagatggt ttcactgaac tatcaaaccc cagatttagc gatgcaattg aatcagggaa
     2281 aatttgagta taatggatcg tgcgggtacc ttctcaaacc agatttcatg aggcggcctg
     2341 atcgaacatt tgaccccttc tctgaaactc ctgttgatgg tgttattgca gccacttgct
     2401 cagtgcaggt tatatcaggt caattcttat cagataagaa aattggcacc tacgtagagg
     2461 tggatatgta tgggttgccc actgacacca tacgtaagga attccgaact cgcatggtta
     2521 tgaataatgg actcaatcca gtttacaatg aagagtcatt tgtatttcgg aaggtgatcc
     2581 tgccggacct ggctgtcttg agaatagctg tgtatgatga taacaacaag ctgattggcc
     2641 agaggatcct cccgcttgat ggcctccaag ccggatatcg acacatttcc cttcgaaatg
     2701 agggaaataa accattatca ctaccaacaa ttttctgcaa tattgttctt aaaacatatg
     2761 tgcctgatgg atttggagat atcgtggatg ctttatcaga tccaaagaaa tttctctcaa
     2821 ttacagaaaa gagagcagac caaatgagag ctatgggcat tgaaactagt gacatagccg
     2881 acgtgcccag tgacacttcc aaaaatgaca agaaaggaaa ggccaacacc gccaaagcaa
     2941 atgtgacccc tcagagtagc tctgagctca gaccaaccac cacggctgcc ctggcctctg
     3001 gtgtggaagc caagaaaggt attgaactta tccctcaagt aaggatagaa gacttaaagc
     3061 agatgaaggc ttacttgaag catttaaaga aacagcagaa ggagctaaat tctttaaaga
     3121 agaaacatgc aaaggaacac agtaccatgc agaagttaca ctgcacgcaa gttgacaaaa
     3181 ttgtggcaca gtatgacaaa gagaagtcga ctcatgagaa aatcctagag aaggcaatga
     3241 agaagaaggg gggaagtaat tgtctcgaaa tgaaaaaaga aacagaaatc aaaattcaga
     3301 cgctgacatc agatcacaaa tctaaggtca aagagattgt agcacagcac acaaaggaat
     3361 ggtcagaaat gatcaatacc cacagtgctg aggagcaaga aatccgagac ctgcacctca
     3421 gccagcagtg tgagctgctg aaaaagctac tcatcaatgc ccacgagcag caaacccagc
     3481 agctgaaact gtcccatgac agggaaagca aggaaatgcg agcacaccag gctaagattt
     3541 ctatggaaaa tagcaaagcc atcagccaag ataaatctat caagaataaa gcagaacggg
     3601 aaaggcgagt cagggagtta aacagcagca acactaaaaa gtttctggaa gaaagaaaga
     3661 gacttgccat gaagcagtcc aaagaaatgg atcagttgaa aaaagtccag cttgaacatc
     3721 tagaattcct agagaaacag aatgagcagc ttttgaaatc ctgtcatgca gtgtcccaaa
     3781 cgcaaggcga aggagatgca gcagatggtg aaattggaag ccgagatgga ccgcagacca
     3841 gcaacagtag tatgaaactc caaaatgcaa actgaagcag caaacccaca aagcatcaaa
     3901 agactcactc acaaacttct gaacacaaac tccatggatg aaagctgttt attttgtttc
     3961 ctttatgtgt aaacaagatg atatctgaaa ccagagagac ttggaatgtc tgactgactt
     4021 ctatttaaca gcttgagtat tgcatttcct tggccaaaca aaaatagcta caaatccaca
     4081 aaaatttact attccagtaa ggcagagtcc aaccattgat aatacaactt aaacatgttt
     4141 gctataaaat accatcacaa gtaaatgagc ttggtgtgaa caactctcct ttgtgatgcc
     4201 ttaggacatg tttgaactgc agcaaaaaac aaaaacaaaa aacagtgcat tagcaatttc
     4261 atagcaagtg catgcactag gaaaagaaaa ctctgtctac aagtttatta gcagaagtgg
     4321 tggtctgcta gacaaataat tttgcaaaat ttttctacat ctaagttacc tcatcagtaa
     4381 gtgccatgtc tctaccatgc catcagaggc taatttcctg taaaagttgt ggaaattgtt
     4441 agaacaatag aaaaatagag cagtgtatgt gtgccaaaac tcatcattac tcaaaggaga
     4501 actgtgttag gcacatttaa gaaagtttac atctgacatt gctttatagg aattgtttct
     4561 gcagattccg gatattataa ttcacaccat aaagattgtg aagtggttat tggcaaacgt
     4621 ttgtaaatgt gaccatgtat aaagtattta tactctttaa ttcacactgt tagagagcaa
     4681 aatcatctaa gtattgccac atgacaagat tagtaaacag gaatactaga actatgtttg
     4741 catgatacac aagcaccaat aaagactaat ccatacacag ttaacctaat gccaaataaa
     4801 tactggttaa ataaatgtat ggcacagaat ataatttgac tatcaagact tttagcataa
     4861 tgaaaaaccc tctctctata tatatatgtg tatatgaatt atgtgggcat tcttgatact
     4921 tcaagttcta gtttgaaaaa aatacataac taatttaatt ttacacaaaa atatttatgc
     4981 agattttcag aatttcatat caggaaatga cctttttatg tctgttaaat atcaaaacaa
     5041 tttgctacag tgttaatctg catggtcttt aagcctgctg tagttgagtt gcagacagtg
     5101 catgaaaaag tattccgctg ggaattgagc catgccacca aagccaagag gagcgcatgg
     5161 aaacccggta gtctagaact aatcagatta ctgattttag ggcacagcac cagatgaatt
     5221 gttgtatatg cttgtaaaaa ttgattctgt gtgttcctct gaacaaagcg gagaaaatga
     5281 tgataccatc aatattgaaa ttaaacttcc aacttctcta ataaaaaatt aaaacacgca
     5341 taacactcgt caagagtatt tgctcccaag acacattcta gcaaatgttt tgcctttttc
     5401 atatacatga tatcatcgtt attttcaaag ggggcttatt aataccctca gcatgttttt
     5461 cacccaaatg atgcaaaaca tgcagattct agttgacttc agttgtaata gacttgtttt
     5521 tctcctattt atgatttgaa gtggattctg taaaatatct cttgttctta gtttccttat
     5581 ctgtaaaaca gtggagttag actacatatc ttttggcact aacatctcat gaaaaattat
     5641 ggttaataaa atatcaccac atttggattg ccaa
//
