LOCUS       NM_015192               7088 bp    mRNA    linear   PRI 01-AUG-2024
DEFINITION  Homo sapiens phospholipase C beta 1 (PLCB1), transcript variant 1,
            mRNA.
ACCESSION   NM_015192
VERSION     NM_015192.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Dai,S., Wang,Q., Lyu,Y., Chen,Z., Liu,X., Zhao,G. and Zhang,H.
  TITLE     LncRNA AC100826.1 regulated PLCB1 to promote progression in
            non-small cell lung cancer
  JOURNAL   Thorac Cancer 15 (19), 1477-1489 (2024)
   PUBMED   38778543
  REMARK    GeneRIF: LncRNA AC100826.1 regulated PLCB1 to promote progression
            in non-small cell lung cancer.
REFERENCE   2  (bases 1 to 7088)
  AUTHORS   Chang,M., Cui,X., Sun,Q., Wang,Y., Liu,J., Sun,Z., Ren,J., Sun,Y.,
            Han,L. and Li,W.
  TITLE     Lnc-PLCB1 is stabilized by METTL14 induced m6A modification and
            inhibits Helicobacter pylori mediated gastric cancer by
            destabilizing DDX21
  JOURNAL   Cancer Lett 588, 216746 (2024)
   PUBMED   38387756
  REMARK    GeneRIF: Lnc-PLCB1 is stabilized by METTL14 induced m6A
            modification and inhibits Helicobacter pylori mediated gastric
            cancer by destabilizing DDX21.
REFERENCE   3  (bases 1 to 7088)
  AUTHORS   Wang,Y., Tu,Z., Zhao,W., Wang,L., Jiang,J., Gu,L., Li,M., Jiang,L.,
            Wang,Y. and Bi,Y.
  TITLE     PLCB1 Enhances Cell Migration and Invasion in Gastric Cancer Via
            Regulating Actin Cytoskeletal Remodeling and Epithelial-Mesenchymal
            Transition
  JOURNAL   Biochem Genet 61 (6), 2618-2632 (2023)
   PUBMED   37208557
  REMARK    GeneRIF: PLCB1 Enhances Cell Migration and Invasion in Gastric
            Cancer Via Regulating Actin Cytoskeletal Remodeling and
            Epithelial-Mesenchymal Transition.
REFERENCE   4  (bases 1 to 7088)
  AUTHORS   Wang,S.H., Cheng,J.Y., Tsai,H.H., Lo,T.C., Hung,J.T., Lin,C.C.,
            Lee,C.W., Ho,Y.H., Kuo,H.H., Yu,A.L. and Yu,J.
  TITLE     Conformational alteration in glycan induces phospholipase Cbeta1
            activation and angiogenesis
  JOURNAL   J Biomed Sci 29 (1), 105 (2022)
   PUBMED   36517806
  REMARK    GeneRIF: Conformational alteration in glycan induces phospholipase
            Cbeta1 activation and angiogenesis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7088)
  AUTHORS   Bao,Y., Guo,H., Yang,B., Chen,F., Zhang,Z. and Gao,J.
  TITLE     MicroRNA-1297 participates in the repair of intestinal barrier
            injury in patients with HIV/AIDS via negative regulation of
            PLCbeta1
  JOURNAL   Mol Cell Biochem 477 (8), 2133-2147 (2022)
   PUBMED   35608718
  REMARK    GeneRIF: MicroRNA-1297 participates in the repair of intestinal
            barrier injury in patients with HIV/AIDS via negative regulation of
            PLCbeta1.
REFERENCE   6  (bases 1 to 7088)
  AUTHORS   Hwang,S.C., Park,K.H., Ha,M.J., Noh,I.S., Park,T.B. and Lee,Y.H.
  TITLE     Distribution of phospholipase C isozymes in normal human lung
            tissue and their immunohistochemical localization
  JOURNAL   J Korean Med Sci 11 (4), 305-313 (1996)
   PUBMED   8878798
REFERENCE   7  (bases 1 to 7088)
  AUTHORS   Zini,N., Sabatelli,P., Faenza,I., Ognibene,A. and Maraldi,N.M.
  TITLE     Interleukin-1 alpha induces variations of the intranuclear amount
            of phosphatidylinositol 4,5-bisphosphate and phospholipase C beta 1
            in human osteosarcoma Saos-2 cells
  JOURNAL   Histochem J 28 (7), 495-504 (1996)
   PUBMED   8872139
REFERENCE   8  (bases 1 to 7088)
  AUTHORS   Zauli,G., Previati,M., Caramelli,E., Bassini,A., Falcieri,E.,
            Gibellini,D., Bertolaso,L., Bosco,D., Robuffo,I. and Capitani,S.
  TITLE     Exogenous human immunodeficiency virus type-1 Tat protein
            selectively stimulates a phosphatidylinositol-specific
            phospholipase C nuclear pathway in the Jurkat T cell line
  JOURNAL   Eur J Immunol 25 (9), 2695-2700 (1995)
   PUBMED   7589147
REFERENCE   9  (bases 1 to 7088)
  AUTHORS   Berstein,G., Blank,J.L., Jhon,D.Y., Exton,J.H., Rhee,S.G. and
            Ross,E.M.
  TITLE     Phospholipase C-beta 1 is a GTPase-activating protein for Gq/11,
            its physiologic regulator
  JOURNAL   Cell 70 (3), 411-418 (1992)
   PUBMED   1322796
REFERENCE   10 (bases 1 to 7088)
  AUTHORS   Banno,Y., Yada,Y. and Nozawa,Y.
  TITLE     Purification and characterization of membrane-bound phospholipase C
            specific for phosphoinositides from human platelets
  JOURNAL   J Biol Chem 263 (23), 11459-11465 (1988)
   PUBMED   2841328
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB011153.3, AY004175.1,
            AL049593.10 and BQ184058.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_015192.3.
            
            Summary: The protein encoded by this gene catalyzes the formation
            of inositol 1,4,5-trisphosphate and diacylglycerol from
            phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium
            as a cofactor and plays an important role in the intracellular
            transduction of many extracellular signals. This gene is activated
            by two G-protein alpha subunits, alpha-q and alpha-11. Two
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB011153.3, SRR1660807.77968.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000338037.11/ ENSP00000338185.6
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3373              AB011153.3         2-3374
            3374-4037           AY004175.1         2973-3636
            4038-5717           AB011153.3         4039-5718
            5718-5718           AL049593.10        36391-36391
            5719-6547           AB011153.3         5720-6548
            6548-7088           BQ184058.1         15-555              c
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p12.3"
     gene            1..7088
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /note="phospholipase C beta 1"
                     /db_xref="GeneID:23236"
                     /db_xref="HGNC:HGNC:15917"
                     /db_xref="MIM:607120"
     exon            1..485
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     CDS             387..4037
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /EC_number="3.1.4.11"
                     /note="isoform a is encoded by transcript variant 1;
                     triphosphoinositide phosphodiesterase; phosphoinositidase
                     C; monophosphatidylinositol phosphodiesterase;
                     1-phosphatidyl-D-myo-inositol-4,5-bisphosphate;
                     inositoltrisphosphohydrolase; phospholipase C-I;
                     1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
                     beta-1; phospholipase C, beta 1
                     (phosphoinositide-specific); phosphoinositide
                     phospholipase C"
                     /codon_start=1
                     /product="1-phosphatidylinositol 4,5-bisphosphate
                     phosphodiesterase beta-1 isoform a"
                     /protein_id="NP_056007.1"
                     /db_xref="CCDS:CCDS13102.1"
                     /db_xref="GeneID:23236"
                     /db_xref="HGNC:HGNC:15917"
                     /db_xref="MIM:607120"
                     /translation="MAGAQPGVHALQLKPVCVSDSLKKGTKFVKWDDDSTIVTPIILR
                     TDPQGFFFYWTDQNKETELLDLSLVKDARCGRHAKAPKDPKLRELLDVGNIGRLEQRM
                     ITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATNLLAQNMSRDAFLEKAYTKLK
                     LQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPEVYRVFLNN
                     LCPRPEIDNIFSEFGAKSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIE
                     KYEPNNSLARKGQISVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTY
                     LTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVI
                     EAIAECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLEKYPLESGV
                     PLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTYSDSSSMFEPSSPGAGEAD
                     TESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKKRNKSFE
                     MSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVAL
                     NFQTMDLAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKI
                     ISGQFLSDKKVGTYVEVDMFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLP
                     TLACLRIAVYEEGGKFIGHRILPVQAIRPGYHYICLRNERNQPLTLPAVFVYIEVKDY
                     VPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKKEADPGETPSEAPSEAR
                     TTPAENGVNHTTTLTPKPPSQALHSQPAPGSVKAPAKTEDLIQSVLTEVEAQTIEELK
                     QQKSFVKLQKKHYKEMKDLVKRHHKKTTDLIKEHTTKYNEIQNDYLRRRAALEKSAKK
                     DSKKKSEPSSPDHGSSTIEQDLAALDAEMTQKLIDLKDKQQQQLLNLRQEQYYSEKYQ
                     KREHIKLLIQKLTDVAEECQNNQLKKLKEICEKEKKELKKKMDKKRQEKITEAKSKDK
                     SQMEEEKTEMIRSYIQEVVQYIKRLEEAQSKRQEKLVEKHKEIRQQILDEKPKLQVEL
                     EQEYQDKFKRLPLEILEFVQEAMKGKISEDSNHGSAPLSLSSDPGKVNHKTPSSEELG
                     GDIPGKEFDTPL"
     exon            486..563
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            564..632
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            633..770
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            771..850
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            851..904
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            905..980
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1081
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1082..1248
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1249..1395
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1396..1553
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1554..1636
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1637..1721
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1722..1899
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1900..1967
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1968..2064
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            2065..2149
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            2150..2274
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            2275..2429
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            2430..2594
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            2595..2694
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            2695..2799
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            2800..2909
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            2910..3042
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            3043..3096
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            3097..3316
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            3317..3497
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            3498..3574
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            3575..3664
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            3665..3722
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            3723..3809
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     exon            3810..7088
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7068..7073
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /note="hexamer: AATAAA"
     polyA_site      7088
                     /gene="PLCB1"
                     /gene_synonym="DEE12; EIEE12; PI-PLC; PLC-154; PLC-beta-1;
                     PLC-I; PLC154; PLCB1A; PLCB1B"
                     /note="major polyA site"
ORIGIN      
        1 actcgctctc tccctctgtg tatagaggat gtgctgaatg gtgcgctttg aggcggcggc
       61 ggcggaggag cagaatccgc cgcgactggc agcctcggct gaccggctcg gcttctcttc
      121 gccttccgag gctcctcatc caccgcgggc tccagacctc gcgtcccgcc cggggcatgg
      181 ccgggcgctg cgcccccgcg cgctctgcct gctgagcggc gccggaggga ggtgcggagg
      241 ccgggaggcc ggggaggccg gcggggagca gagtcgagcg cctccggagc agagaaagga
      301 gcccgcgccc cgcgccccgc gccccgcgca cggtccccag tccctgccgc gctcgcccgg
      361 gccgcccgga gcccagatga gcccagatgg ccggggctca acccggagtg cacgccttgc
      421 aactcaagcc cgtgtgcgtg tccgacagcc tcaagaaggg caccaaattc gtcaagtggg
      481 atgatgactc aactattgtt actccaatta ttttgaggac tgaccctcag ggatttttct
      541 tttactggac agatcaaaac aaggagacag agctactgga tctcagcctt gtcaaagatg
      601 ccagatgtgg gagacacgcc aaagctccca aggaccccaa attacgtgaa cttttggatg
      661 tggggaacat cgggcgcctg gagcagcgca tgatcacagt ggtgtatggg cctgacctcg
      721 tgaacatctc ccatttgaat ctcgtggctt tccaagaaga agtggccaag gaatggacaa
      781 atgaggtttt cagtttggca acaaacctgc tggcccaaaa catgtccagg gatgcatttc
      841 tggaaaaagc ctatactaaa cttaagctgc aagtcactcc agaagggcgt attcctctca
      901 aaaacatata tcgcttgttt tcagcagatc ggaagcgagt tgaaactgct ttagaggctt
      961 gtagtcttcc atcttcaagg aatgattcaa tacctcaaga agatttcact ccagaagtgt
     1021 acagagtttt cctcaacaac ctttgccctc gacctgaaat tgataacatc ttttcagaat
     1081 ttggtgcaaa aagcaaacca tatcttaccg ttgatcagat gatggatttt atcaacctta
     1141 agcagcgaga tcctcggctt aatgaaatac tttatccacc tctaaaacaa gagcaagtcc
     1201 aagtattgat tgagaagtat gaacccaaca acagcctcgc cagaaaagga caaatatcag
     1261 tggatgggtt catgcgctat ctgagtggag aagaaaacgg agtcgtttca cctgagaaac
     1321 tggatttgaa tgaagacatg tctcagcccc tttctcacta tttcattaat tcctcgcaca
     1381 acacctacct cacagctggc caactggctg gaaactcctc tgttgagatg tatcgccaag
     1441 tgctcctgtc tggttgtcgc tgtgtggagc tggactgctg gaagggacgg actgcagaag
     1501 aggaacctgt catcacccat ggcttcacca tgacaactga aatatctttc aaggaagtga
     1561 tagaagcaat tgcggagtgt gcatttaaga cttcaccttt tccaattctc ctttcgtttg
     1621 agaaccatgt ggattcccca aagcagcaag ccaagatggc ggagtactgc cgactgatct
     1681 ttggggatgc ccttctcatg gagcccctgg aaaaatatcc actggaatct ggagttcctc
     1741 ttccaagccc tatggattta atgtataaaa ttttggtgaa aaataagaag aaatcacaca
     1801 agtcatcaga aggaagcggc aaaaagaagc tctcagaaca agcctccaac acctacagtg
     1861 actcctccag catgttcgag ccctcatccc caggagccgg agaagctgat acggaaagtg
     1921 acgacgacga tgatgatgat gactgtaaaa aatcttcaat ggatgagggg actgctggaa
     1981 gtgaggctat ggccacagaa gaaatgtcta atctggtgaa ctatattcag ccagtcaagt
     2041 ttgagtcatt tgaaatttca aaaaaaagaa ataaaagttt tgaaatgtct tccttcgtgg
     2101 aaaccaaagg acttgaacaa ctcaccaagt ctccagtgga atttgtagaa tataacaaaa
     2161 tgcagcttag caggatatat ccaaaaggaa cacgtgtgga ttcatccaac tatatgcctc
     2221 agctcttctg gaatgcaggt tgtcagatgg tggcacttaa tttccagaca atggacctgg
     2281 ctatgcaaat aaatatgggg atgtatgaat acaacgggaa gagtggctac agattgaagc
     2341 cagagttcat gaggaggcct gacaagcatt ttgatccatt tactgaaggc atcgtagatg
     2401 ggatagtggc aaacactttg tctgttaaga ttatttcagg tcagtttctt tctgataaga
     2461 aagttgggac ttacgtggaa gtagatatgt ttggtttgcc tgtggataca aggaggaagg
     2521 catttaagac caaaacatcc caaggaaatg ctgtgaatcc tgtctgggaa gaagaaccta
     2581 ttgtgttcaa aaaggtggtt cttcctactc tggcctgttt gagaatagca gtttatgaag
     2641 aaggaggtaa attcattggc caccgtatct tgccagtgca agccattcgg ccaggctatc
     2701 actatatctg tctaaggaat gaaaggaacc agcctctgac gctgcctgct gtctttgtct
     2761 acatagaagt gaaagactat gtgccagaca catatgcaga tgtcatcgaa gctttatcaa
     2821 acccaatccg atatgtgaac ctgatggaac agagagctaa gcaattggct gctttgacac
     2881 tggaagatga agaagaagta aagaaagagg ctgatcctgg agaaacacca tcagaggctc
     2941 caagtgaagc gagaacgact ccagcagaaa atggggtgaa tcacactaca accctgacac
     3001 ccaagccacc ctcccaggct ctccacagcc agccagctcc aggttctgta aaggcacctg
     3061 ccaaaacaga agatcttatt cagagtgtct taacagaagt ggaagcacag accatcgaag
     3121 aactaaagca acagaaatcg tttgtgaaac ttcaaaagaa acactacaaa gaaatgaaag
     3181 acctggttaa gagacaccac aagaaaacca ctgaccttat caaagaacac actaccaagt
     3241 ataatgaaat tcagaatgac tacttgagaa ggagagccgc tttggaaaag tccgccaaaa
     3301 aggacagtaa gaaaaaatcg gaacccagca gccctgatca tggttcatca acgattgagc
     3361 aagacctcgc tgctctggat gctgaaatga cccaaaagtt aatagacttg aaggacaaac
     3421 aacagcagca gctgcttaat cttcggcaag aacagtatta tagtgaaaaa taccagaagc
     3481 gagaacatat taaactgctt attcaaaagt tgacggatgt cgcagaagag tgtcagaaca
     3541 atcagttaaa gaagctcaaa gaaatctgtg agaaagaaaa gaaagaatta aagaagaaaa
     3601 tggataaaaa gaggcaggag aagataacag aagctaaatc caaagacaaa agtcagatgg
     3661 aagaggagaa gacagagatg atccggtcat atatccagga agtggtgcag tatatcaaga
     3721 ggctagaaga agcgcaaagt aaacggcaag aaaaactcgt agagaaacac aaggaaatac
     3781 gtcagcagat cctggatgaa aagcccaagc tgcaggtgga gctggagcaa gaataccaag
     3841 acaaattcaa aagactgccc ctcgagattt tggaattcgt gcaggaagcc atgaaaggaa
     3901 agatcagtga agacagcaat cacggttctg cccctctctc cctgtcctca gaccctggaa
     3961 aagtgaacca caagactccc tccagtgagg agctgggagg agacatccca ggaaaagaat
     4021 ttgatactcc tctgtgaatg ctcctgccag gccttcagaa attgcatggc cactccagcg
     4081 tcatcggact ctctcttatt acaaagatca ctgcccagga ccatcttccc gagaagcatc
     4141 ccttagccta aaatccacac caaagggaga gttccagagg aatccatgaa gaattcccat
     4201 gcccaggctc catgtgtcat gtggaaacct ccacaggtct gctagtgaag aatgcatgta
     4261 tgtgagattt ttgttttctt tccaatagca aattcaaagc aagcaacttg caggctccat
     4321 ggaactttta atgaaggaca gtgtcttctt tgaagaaaat caagctcgtg tttttattcg
     4381 aagctctggt gtaaaatatt tcaaagtcat agaaatagtt tgagaaatgc atagcattat
     4441 ttaacactat tgaacagccg actttgagca ttgtttcttc taactgcccc tcaactacca
     4501 ttatcttcaa gtcaacatgc atattacatt ttcatccttt gctttgcaag cactggtggc
     4561 ttgcagtttg ctaatttatt tatcatagag tcatcaatgt atttgttgct gacatggttt
     4621 tattagatac tgtagtgatt caaataagtt ttctatttga aaaaaaaaat cacttgattg
     4681 tatccttgcc cagtgaagcc atcctaagac ttagcaatat ggattgtaca tttggctgca
     4741 tgagcaagtc ggccgcacac ttccagacag tgtgctgttt gaattgacta tttgcactca
     4801 aagtctgggt attcattggt tattggcctg aaatgatcaa ataactacaa atgatctgtt
     4861 gaataaaaat agttgagctg atatatgtta agcagatatt caatcagaat gaacaggttc
     4921 cggtggttat tttgccgttt gacatttttt atggttcatt tatttttaat atagagagga
     4981 agattgaata tttatctaga gaatacaaag acccacatgt aaatgatagg tattatctcc
     5041 atgtatatat gtacccactt agtcatgtaa gtgcatatac acatacacac acatgagtgt
     5101 agacatgtgt ttattaattg acaatgaccc aaatctcttc cacaagactt aaaaccaaat
     5161 tcagggacaa atggatagag aagaaaaggg tcaaacatcg agattacatg gatgttaaat
     5221 tatatggaga cgctaagaaa taattgatgg agccattgat gcaaaccgaa gtagatttag
     5281 aacttatatg aatttgattt atattttgca agatcaaaaa ttagatgtta agtatcagat
     5341 tttaagcttg ttttaatggt caaaaaatta ggacagaaat aatatggaca tttattagta
     5401 tcttccataa tttttaagtc tgacacattt ctattttatt ctaacataaa aaaacttcca
     5461 ttatatattt actaagtata tttaattcac ttaactctgt ctttataagt tcctatttta
     5521 gatggaataa gagaaacaaa ttatatcaag gtaaaactga tcaaaagcat aattgaaagt
     5581 tctgaaaaaa gaaaaataat aatatgtaga aaaatgtaac ttagagagta acacatgaac
     5641 attgaagtta aaacccagaa gccagatgct cacagtttta ttttacttta aaataaacct
     5701 gtctgactgt agctttgtga aatatcttaa aacgcaaaaa ccaattgtgt cctgaaaatt
     5761 gtttcaagaa tttaatattt ttatgaaaat tattttattt aactttaagc aataactaga
     5821 gattacaatt aaattttaat caaaatgaag gcttagttca aacataagga aacagtgttt
     5881 gattaaaaaa aacacatcta gtaagacgta aggggaaaat cacatcctct ttggagatga
     5941 ttatattttg atctgaaggg tttggggtgt tcattagaca cttaataaaa cttaacttcc
     6001 aatgaaaaag aaatcttttg taaatcattc tacttttgca ctttgaaaga aaggcgttaa
     6061 tcataaagaa gcaagaatgg tcaaaatcga atgctgcatt tttataaaca aaattacaga
     6121 ctgtctgaaa ttgaagaaga atgaactaat aatagcattc ataaccaaac acaatgatga
     6181 ttattgcaga acattgtatc acattttagt ccagagatag aataaagttg aataaccttg
     6241 acttacacaa aactgttttg gtagttggat ttcattatct tagtgaattt agtcatttta
     6301 caatatgttt gtatttggcc atttactgta atcacatttt tatatctgta caatgacact
     6361 ttttgcagtt gtggggtagt gtgtaacact gtccatcttg catcattgaa actactacaa
     6421 tgatactatc atttaataat attaatatta cttgaaatag actaagataa agaaaagggg
     6481 tctgtatgat gtgcagtttt gtgcctttat gtatttgcct tgttctttgt cgaatgtgtg
     6541 aaattccgta ctgtggtttt tcctataata gaaagtagag ctgtgtatta aattagactg
     6601 tgtctctctg atacctttac actactgaga atagcatggt tttggccatg taaaccaatt
     6661 ttcaaagttc taatgacata gccatgtgtt tttggttttt tatatttcat tttaaaattt
     6721 gagtatcacc atacattaat taatactcct gtagtagata agctgtcatt aagtaattcc
     6781 caaaaaaagg gccatttgct tgcattactt tgaatttaat gttgcgcttg tgcactgtgt
     6841 taatattgtt tgtgatggat tggacgttgt gactcttgcc ttttaagaag aaaaaaaaga
     6901 taggacaaag tatttgaagc tcttaaaatg tacatatttt ggttcttcta tctcaaatta
     6961 tttaaaatgc ataattcaca tttttgtaat aattctatgc aattttgtgg catgatgttt
     7021 cttccacttg taattttatg tgctttcatc acaaatccaa aggaaagaat aaaaatttct
     7081 taacacaa
//
