LOCUS       NP_775813                589 aa            linear   PRI 28-APR-2025
DEFINITION  putative phospholipase B-like 2 isoform 1 precursor [Homo sapiens].
ACCESSION   NP_775813
VERSION     NP_775813.2
DBSOURCE    REFSEQ: accession NM_173542.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 589)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (residues 1 to 589)
  AUTHORS   Jensen,A.G., Chemali,M., Chapel,A., Kieffer-Jaquinod,S., Jadot,M.,
            Garin,J. and Journet,A.
  TITLE     Biochemical characterization and lysosomal localization of the
            mannose-6-phosphate protein p76 (hypothetical protein LOC196463)
  JOURNAL   Biochem J 402 (3), 449-458 (2007)
   PUBMED   17105447
  REMARK    GeneRIF: describe the identification of p76 by MS and we analyze
            several of its biochemical characteristics
REFERENCE   3  (residues 1 to 589)
  AUTHORS   Morgan,C.P., Insall,R., Haynes,L. and Cockcroft,S.
  TITLE     Identification of phospholipase B from Dictyostelium discoideum
            reveals a new lipase family present in mammals, flies and
            nematodes, but not yeast
  JOURNAL   Biochem J 382 (Pt 2), 441-449 (2004)
   PUBMED   15193148
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC030618.2, AC010178.40 and CN412955.1.
            
            On May 1, 2009 this sequence version replaced NP_775813.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.117570.1, BC030618.2
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000280800.5/ ENSP00000280800.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.13"
     Protein         1..589
                     /product="putative phospholipase B-like 2 isoform 1
                     precursor"
                     /note="PLB homolog 2; mannose-6-phosphate protein
                     associated protein p76; lamina ancestor homolog 2;
                     LAMA-like protein 2; phospholipase B-like 2 32 kDa form;
                     phospholipase B-like 2 45 kDa form; putative phospholipase
                     B-like 2; 76 kDa protein; phospholipase B
                     domain-containing protein 2"
                     /calculated_mol_wt=61303
     sig_peptide     1..41
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=4187
     mat_peptide     42..589
                     /product="putative phospholipase B-like 2 isoform 1"
                     /calculated_mol_wt=61303
     mat_peptide     42..243
                     /product="Putative phospholipase B-like 2 32 kDa form.
                     /id=PRO_0000314074"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8NHP8.2)"
                     /calculated_mol_wt=22383
     Region          85..584
                     /region_name="Phospholip_B"
                     /note="Phospholipase B; pfam04916"
                     /db_xref="CDD:461479"
     Site            88
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000269|PubMed:17105447; propagated from
                     UniProtKB/Swiss-Prot (Q8NHP8.2)"
     Site            110
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000269|PubMed:17105447; propagated from
                     UniProtKB/Swiss-Prot (Q8NHP8.2)"
     Site            231
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000269|PubMed:17105447; propagated from
                     UniProtKB/Swiss-Prot (Q8NHP8.2)"
     mat_peptide     244..589
                     /product="Putative phospholipase B-like 2 45 kDa form.
                     /id=PRO_0000314075"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8NHP8.2)"
                     /calculated_mol_wt=38938
     Site            436
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000269|PubMed:17105447; propagated from
                     UniProtKB/Swiss-Prot (Q8NHP8.2)"
     Site            465
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000269|PubMed:17105447,
                     ECO:0000269|PubMed:19159218; propagated from
                     UniProtKB/Swiss-Prot (Q8NHP8.2)"
     Site            515
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000269|PubMed:17105447; propagated from
                     UniProtKB/Swiss-Prot (Q8NHP8.2)"
     CDS             1..589
                     /gene="PLBD2"
                     /gene_synonym="P76"
                     /coded_by="NM_173542.4:15..1784"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1"
                     /db_xref="CCDS:CCDS9168.1"
                     /db_xref="GeneID:196463"
                     /db_xref="HGNC:HGNC:27283"
                     /db_xref="MIM:620097"
ORIGIN      
        1 mvgqmycypg shlaraltra lalalvlall vgpflsglag aipapggrwa rdgqvppasr
       61 srsvlldvsa gqllmvdgrh pdavawanlt nairetgwaf lelgtsgqyn dslqayaagv
      121 veaavseeli ymhwmntvvn ycgpfeyevg ycerlksfle anlewmqeem esnpdspywh
      181 qvrltllqlk gledsyegrv sfpagkftik plgflllqls gdledlelal nktkikpslg
      241 sgscsalikl lpgqsdllva hntwnnyqhm lrvikkywlq fregpwgdyp lvpgnklvfs
      301 sypgtifscd dfyilgsglv tlettignkn palwkyvrpr gcvlewvrni vanrlasdga
      361 twadifkrfn sgtynnqwmi vdykafipgg pspgsrvlti leqipgmvvv adktselyqk
      421 tywasynips fetvfnasgl qalvaqygdw fsydgspraq ifrrnqslvq dmdsmvrlmr
      481 yndflhdpls lckacnpqpn genaisarsd lnpangsypf qalrqrshgg idvkvtsmsl
      541 arilsllaas gptwdqvppf qwstspfsgl lhmgqpdlwk fapvkvswd
//
