LOCUS       NM_153021               5513 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens phospholipase B1 (PLB1), transcript variant 1, mRNA.
ACCESSION   NM_153021 XM_371487
VERSION     NM_153021.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 5513)
  AUTHORS   Zhu,M., Geng,L., Shen,W., Wang,Y., Liu,J., Cheng,Y., Wang,C.,
            Dai,J., Jin,G., Hu,Z., Ma,H. and Shen,H.
  TITLE     Exome-Wide Association Study Identifies Low-Frequency Coding
            Variants in 2p23.2 and 7p11.2 Associated with Survival of Non-Small
            Cell Lung Cancer Patients
  JOURNAL   J Thorac Oncol 12 (4), 644-656 (2017)
   PUBMED   28104536
  REMARK    GeneRIF: rs117512489 in gene phospholipase B1 gene is associated
            with Non-Small Cell Lung Cancer.
REFERENCE   4  (bases 1 to 5513)
  AUTHORS   Okada,Y., Diogo,D., Greenberg,J.D., Mouassess,F., Achkar,W.A.,
            Fulton,R.S., Denny,J.C., Gupta,N., Mirel,D., Gabriel,S., Li,G.,
            Kremer,J.M., Pappas,D.A., Carroll,R.J., Eyler,A.E., Trynka,G.,
            Stahl,E.A., Cui,J., Saxena,R., Coenen,M.J., Guchelaar,H.J.,
            Huizinga,T.W., Dieude,P., Mariette,X., Barton,A., Canhao,H.,
            Fonseca,J.E., de Vries,N., Tak,P.P., Moreland,L.W., Bridges,S.L.
            Jr., Miceli-Richard,C., Choi,H.K., Kamatani,Y., Galan,P.,
            Lathrop,M., Raj,T., De Jager,P.L., Raychaudhuri,S., Worthington,J.,
            Padyukov,L., Klareskog,L., Siminovitch,K.A., Gregersen,P.K.,
            Mardis,E.R., Arayssi,T., Kazkaz,L.A. and Plenge,R.M.
  TITLE     Integration of sequence data from a Consanguineous family with
            genetic data from an outbred population identifies PLB1 as a
            candidate rheumatoid arthritis risk gene
  JOURNAL   PLoS One 9 (2), e87645 (2014)
   PUBMED   24520335
  REMARK    GeneRIF: PLB1 is a candidate risk gene for rheumatoid arthritis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5513)
  AUTHORS   Payne,A.H. and Hales,D.B.
  TITLE     Overview of steroidogenic enzymes in the pathway from cholesterol
            to active steroid hormones
  JOURNAL   Endocr Rev 25 (6), 947-970 (2004)
   PUBMED   15583024
  REMARK    Review article
REFERENCE   6  (bases 1 to 5513)
  AUTHORS   Maury,E., Prevost,M.C., Nauze,M., Redoules,D., Tarroux,R.,
            Charveron,M., Salles,J.P., Perret,B., Chap,H. and Gassama-Diagne,A.
  TITLE     Human epidermis is a novel site of phospholipase B expression
  JOURNAL   Biochem Biophys Res Commun 295 (2), 362-369 (2002)
   PUBMED   12150957
  REMARK    GeneRIF: identification of epidermis as a site of expression
REFERENCE   7  (bases 1 to 5513)
  AUTHORS   Rigtrup,K.M., McEwen,L.R., Said,H.M. and Ong,D.E.
  TITLE     Retinyl ester hydrolytic activity associated with human intestinal
            brush border membranes
  JOURNAL   Am J Clin Nutr 60 (1), 111-116 (1994)
   PUBMED   8017323
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC093164.6, BC150633.1,
            BC153864.1, AC074011.5 and BX109719.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_153021.4.
            
            Summary: This gene encodes a membrane-associated phospholipase that
            displays lysophospholipase and phospholipase A2 activities through
            removal of sn-1 and sn-2 fatty acids of glycerophospholipids. In
            addition, it displays lipase and retinyl ester hydrolase
            activities. The encoded protein is highly conserved and is composed
            of a large, glycosylated extracellular domain composed of four
            tandem homologous domains, followed by a hydrophobic segment that
            anchors the enzyme to the membrane and a short C-terminal
            cytoplasmic tail. This gene has been identified as a candidate
            rheumatoid arthritis risk gene. [provided by RefSeq, Jul 2016].
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC150633.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000327757.10/ ENSP00000330442.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                AC093164.6         57429-57439
            12-1388             BC150633.1         1-1377
            1389-1389           BC153864.1         1363-1363
            1390-2118           BC150633.1         1379-2107
            2119-2177           BC153864.1         2027-2085
            2178-4264           BC150633.1         2167-4253
            4265-5118           AC074011.5         102336-103189
            5119-5159           BX109719.1         621-661
            5160-5513           AC074011.5         103231-103584
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p23.2"
     gene            1..5513
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /note="phospholipase B1"
                     /db_xref="GeneID:151056"
                     /db_xref="HGNC:HGNC:30041"
                     /db_xref="MIM:610179"
     exon            1..110
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     CDS             56..4432
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /EC_number="3.1.1.3"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.1.4"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; phospholipase B1, membrane-associated;
                     lysophospholipase; phospholipase A2; phospholipase
                     B/lipase; triacylglycerol lipase"
                     /codon_start=1
                     /product="phospholipase B1, membrane-associated isoform 1
                     precursor"
                     /protein_id="NP_694566.4"
                     /db_xref="CCDS:CCDS33168.1"
                     /db_xref="GeneID:151056"
                     /db_xref="HGNC:HGNC:30041"
                     /db_xref="MIM:610179"
                     /translation="MGLRPGIFLLELLLLLGQGTPQIHTSPRKSTLEGQLWPETLKNS
                     PFPCNPNKLGVNMPSKSVHSLKPSDIKFVAAIGNLEIPPDPGTGDLEKQDWTERPQQV
                     CMGVMTVLSDIIRYFSPSVPMPVCHTGKRVIPHDGAEDLWIQAQELVRNMKENLQLDF
                     QFDWKLINVFFSNASQCYLCPSAQQNGLAAGGVDELMGVLDYLQQEVPRAFVNLVDLS
                     EVAEVSRQYHGTWLSPAPEPCNCSEETTRLAKVVMQWSYQEAWNSLLASSRYSEQESF
                     TVVFQPFFYETTPSLHSEDPRLQDSTTLAWHLWNRMMEPAGEKDEPLSVKHGRPMKCP
                     SQESPYLFSYRNSNYLTRLQKPQDKLEVREGAEIRCPDKDPSDTVPTSVHRLKPADIN
                     VIGALGDSLTAGNGAGSTPGNVLDVLTQYRGLSWSVGGDENIGTVTTLANILREFNPS
                     LKGFSVGTGKETSPNAFLNQAVAGGRAEDLPVQARRLVDLMKNDTRIHFQEDWKIITL
                     FIGGNDLCDFCNDLVHYSPQNFTDNIGKALDILHAEVPRAFVNLVTVLEIVNLRELYQ
                     EKKVYCPRMILRSLCPCVLKFDDNSTELATLIEFNKKFQEKTHQLIESGRYDTREDFT
                     VVVQPFFENVDMPKTSEGLPDNSFFAPDCFHFSSKSHSRAASALWNNMLEPVGQKTTR
                     HKFENKINITCPNQVQPFLRTYKNSMQGHGTWLPCRDRAPSALHPTSVHALRPADIQV
                     VAALGDSLTAGNGIGSKPDDLPDVTTQYRGLSYSAGGDGSLENVTTLPNILREFNRNL
                     TGYAVGTGDANDTNAFLNQAVPGAKAEDLMSQVQTLMQKMKDDHRVNFHEDWKVITVL
                     IGGSDLCDYCTDSNLYSAANFVHHLRNALDVLHREVPRVLVNLVDFLNPTIMRQVFLG
                     NPDKCPVQQASVLCNCVLTLRENSQELARLEAFSRAYRSSMRELVGSGRYDTQEDFSV
                     VLQPFFQNIQLPVLADGLPDTSFFAPDCIHPNQKFHSQLARALWTNMLEPLGSKTETL
                     DLRAEMPITCPTQNEPFLRTPRNSNYTYPIKPAIENWGSDFLCTEWKASNSVPTSVHQ
                     LRPADIKVVAALGDSLTTAVGARPNNSSDLPTSWRGLSWSIGGDGNLETHTTLPNILK
                     KFNPYLLGFSTSTWEGTAGLNVAAEGARARDMPAQAWDLVERMKNSPDINLEKDWKLV
                     TLFIGVNDLCHYCENPEAHLATEYVQHIQQALDILSEELPRAFVNVVEVMELASLYQG
                     QGGKCAMLAAQNNCTCLRHSQSSLEKQELKKVNWNLQHGISSFSYWHQYTQREDFAVV
                     VQPFFQNTLTPLNERGDTDLTFFSEDCFHFSDRGHAEMAIALWNNMLEPVGRKTTSNN
                     FTHSRAKLKCPSPESPYLYTLRNSRLLPDQAEEAPEVLYWAVPVAAGVGLVVGIIGTV
                     VWRCRRGGRREDPPMSLRTVAL"
     exon            111..172
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            173..239
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            240..298
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            299..339
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            340..380
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            381..471
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            472..523
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            524..610
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            611..673
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            674..753
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            754..829
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            830..934
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            935..991
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            992..1063
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1064..1138
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1139..1202
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1203..1261
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1262..1335
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1336..1379
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1380..1488
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1489..1540
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..1621
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1622..1687
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1688..1788
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1789..1870
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1871..1975
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1976..2071
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            2072..2143
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            2144..2182
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            2183..2243
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            2244..2302
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            2303..2376
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            2377..2420
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            2421..2529
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            2530..2581
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            2582..2662
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            2663..2728
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            2729..2829
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            2830..2911
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            2912..3016
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            3017..3112
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            3113..3184
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            3185..3250
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            3251..3311
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            3312..3370
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            3371..3438
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            3439..3482
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            3483..3582
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            3583..3634
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            3635..3715
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            3716..3781
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            3782..3873
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            3874..3952
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            3953..4057
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            4058..4153
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            4154..4228
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     exon            4229..5513
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4617..4622
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /note="hexamer: AATAAA"
     polyA_site      4638
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /note="major polyA site"
     regulatory      5136..5141
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /note="hexamer: AATAAA"
     polyA_site      5159
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /note="major polyA site"
     regulatory      5483..5488
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
                     /note="hexamer: AATAAA"
     polyA_site      5513
                     /gene="PLB1"
                     /gene_synonym="PLB; PLB/LIP"
ORIGIN      
        1 atccatctgc tggagcagct cttccagagg cccgagtcac ctggagcatt ctggcatggg
       61 gctgcggcca ggcattttcc tcctggagct gctgctgctt ctggggcaag ggacccctca
      121 gatccatacc tctcctagaa agagtacatt ggaagggcag ctatggccag agaccctgaa
      181 gaattctcca ttcccatgca acccaaataa attaggagtg aatatgcctt ctaaatcagt
      241 tcactctctg aagccttctg atattaaatt tgtggcagcc attggcaatc tggaaattcc
      301 tccagaccca gggacgggcg atctggagaa gcaagactgg actgaaaggc cacagcaggt
      361 gtgcatggga gtgatgacag tcctttcaga catcatcaga tatttcagtc cttctgttcc
      421 aatgcctgtg tgccacactg gaaagagagt cataccccac gatggtgctg aagacttgtg
      481 gattcaggct caagaactgg tgagaaacat gaaagagaac ctgcaacttg actttcaatt
      541 tgactggaag ctcatcaatg tgttcttcag taatgcaagc cagtgttacc tgtgcccctc
      601 tgctcaacag aatgggcttg cggcgggcgg cgtggatgag ctgatggggg tgctggacta
      661 cctgcagcag gaggtcccca gagcatttgt aaacctggtg gacctctctg aggttgcaga
      721 ggtctctcgt cagtatcacg gcacttggct cagccctgca ccagagccct gtaattgctc
      781 agaggagacc acccggctgg ccaaggtggt gatgcagtgg tcttatcagg aagcctggaa
      841 cagcctcctg gcctccagca ggtacagtga gcaggagtcc ttcaccgtgg ttttccagcc
      901 tttcttctat gagaccaccc catctctaca ctcggaggac ccccgactcc aggattctac
      961 cacgctggcc tggcatctct ggaataggat gatggagcca gcaggagaga aagatgagcc
     1021 attgagtgta aaacacggga ggccaatgaa gtgtccctct caggagagcc cctatctgtt
     1081 cagctacaga aacagcaact acctgaccag actgcagaaa ccccaagaca agcttgaggt
     1141 aagagaagga gcggaaatca gatgtcctga caaagacccc tccgatacgg ttcccacctc
     1201 agttcatagg ctgaagccgg ctgacatcaa cgtaattgga gccctgggtg actctctcac
     1261 ggcaggcaat ggggccgggt ccacacctgg gaacgtcttg gacgtcttga ctcagtaccg
     1321 aggcctgtcc tggagcgtcg gcggagatga gaacatcggc accgttacca ccctggcgaa
     1381 catcctccgg gaattcaacc cttccctgaa gggcttctct gttggcactg ggaaagaaac
     1441 cagtcctaat gccttcttaa accaggctgt ggcaggaggc cgagctgagg atctacctgt
     1501 ccaggccagg aggctggtgg acctgatgaa gaatgacacg aggatacact ttcaggaaga
     1561 ctggaagata ataaccctgt ttataggcgg caatgacctc tgtgatttct gcaatgatct
     1621 ggtccactat tctccccaga acttcacaga caacattgga aaggccctgg acatcctcca
     1681 tgctgaggtt cctcgggcat ttgtgaacct ggtgacggtg cttgagatcg tcaacctgag
     1741 ggagctgtac caggagaaaa aagtctactg cccaaggatg atcctcaggt ctctgtgtcc
     1801 ctgtgtcctg aagtttgatg ataactcaac agaacttgct accctcatcg aattcaacaa
     1861 gaagtttcag gagaagaccc accaactgat tgagagtggg cgatatgaca caagggaaga
     1921 ttttactgtg gttgtgcagc cgttctttga aaacgtggac atgccaaaga cctcggaagg
     1981 attgcctgac aactctttct tcgctcctga ctgtttccac ttcagcagca agtctcactc
     2041 ccgagcagcc agtgctctct ggaacaatat gctggagcct gttggccaga agacgactcg
     2101 tcataagttt gaaaacaaga tcaatatcac atgtccgaac caggtccagc cgtttctgag
     2161 gacctacaag aacagcatgc agggtcatgg gacctggctg ccatgcaggg acagagcccc
     2221 ttctgccttg caccctacct cagtgcatgc cctgagacct gcagacatcc aagttgtggc
     2281 tgctctgggg gattctctga ccgctggcaa tggaattggc tccaaaccag acgacctccc
     2341 cgatgtcacc acacagtatc ggggactgtc atacagtgca ggaggggacg gctccctgga
     2401 gaatgtgacc accttaccta atatccttcg ggagtttaac agaaacctca caggctacgc
     2461 cgtgggcacg ggtgatgcca atgacacgaa tgcattcctc aatcaagctg ttcccggagc
     2521 aaaggctgag gatcttatga gccaagtcca aactctgatg cagaagatga aagatgatca
     2581 tagagtaaat ttccatgaag actggaaggt catcacagtg ctgatcggag gcagcgattt
     2641 atgtgactac tgcacagatt cgaatctgta ttctgcagcc aactttgttc accatctccg
     2701 caatgccttg gacgtcctgc atagagaggt gcccagagtc ctggtcaacc tcgtggactt
     2761 cctgaacccc actatcatgc ggcaggtgtt cctgggaaac ccagacaagt gcccagtgca
     2821 gcaggccagc gttttgtgta actgcgttct gaccctgcgg gagaactccc aagagctagc
     2881 caggctggag gccttcagcc gagcctaccg gagcagcatg cgcgagctgg tggggtcagg
     2941 ccgctatgac acgcaggagg acttctctgt ggtgctgcag cccttcttcc agaacatcca
     3001 gctccctgtc ctggcggatg ggctcccaga tacgtccttc tttgccccag actgcatcca
     3061 cccaaatcag aaattccact cccagctggc cagagccctt tggaccaata tgcttgaacc
     3121 acttggaagc aaaacagaga ccctggacct gagagcagag atgcccatca cctgtcccac
     3181 tcagaatgag cccttcctga gaacccctcg gaatagtaac tacacgtacc ccatcaagcc
     3241 agccattgag aactggggca gtgacttcct gtgtacagag tggaaggctt ccaatagtgt
     3301 tccaacctct gtccaccagc tccgaccagc agacatcaaa gtggtggccg ccctgggtga
     3361 ctctctgact acagcagtgg gagctcgacc aaacaactcc agtgacctac ccacatcttg
     3421 gaggggactc tcttggagca ttggagggga tgggaacttg gagactcaca ccacactgcc
     3481 caacattctg aagaagttca acccttacct ccttggcttc tctaccagca cctgggaggg
     3541 gacagcagga ctaaatgtgg cagcggaagg ggccagagct agggacatgc cagcccaggc
     3601 ctgggacctg gtagagcgaa tgaaaaacag ccccgacatc aacctggaga aagactggaa
     3661 gctggtcaca ctcttcattg gggtcaacga cttgtgtcat tactgtgaga atccggaggc
     3721 ccacttggcc acggaatatg ttcagcacat ccaacaggcc ctggacatcc tctctgagga
     3781 gctcccaagg gctttcgtca acgtggtgga ggtcatggag ctggctagcc tgtaccaggg
     3841 ccaaggcggg aaatgtgcca tgctggcagc tcagaacaac tgcacttgcc tcagacactc
     3901 gcaaagctcc ctggagaagc aagaactgaa gaaagtgaac tggaacctcc agcatggcat
     3961 ctccagtttc tcctactggc accaatacac acagcgtgag gactttgcgg ttgtggtgca
     4021 gcctttcttc caaaacacac tcaccccact gaacgagaga ggggacactg acctcacctt
     4081 cttctccgag gactgttttc acttctcaga ccgcgggcat gccgagatgg ccatcgcact
     4141 ctggaacaac atgctggaac cagtgggccg caagactacc tccaacaact tcacccacag
     4201 ccgagccaaa ctcaagtgcc cctctcctga gagcccttac ctctacaccc tgcggaacag
     4261 ccgattgctc ccagaccagg ctgaagaagc ccccgaggtg ctctactggg ctgtcccagt
     4321 ggcagcggga gtcggccttg tggtgggcat catcgggaca gtggtctgga ggtgcaggag
     4381 aggtggccgg agggaagatc ctccaatgag cctgcgcact gtggccctct aggcccgggg
     4441 gtgggtcctc accctaaact ccctatagcc actctcttca ccgccctctg ccccagccac
     4501 tcccggccac caggacatgc ttcaatgcct ggtgccatag gaagcccagg ggacagtcac
     4561 aacttcttgg ggcctgggct tcttccaggc ctatgctcct ggaatggata catttaaata
     4621 aagtccaaag ctattttatt cctgggtttg cctgcgtgaa gcactcacct tccatctctt
     4681 gtgcagccca ggtgtgggag ctgccacttt ttgtggcctg cctccagcag ggctgcccaa
     4741 gccacgacca accagagccc aaactgcctg ccaccacgag catatcctca agtcaccaaa
     4801 cccactattt caaaggcaga aaaaatgctg gtcaccaggt ggtggctgga attttggagc
     4861 tggctggttg ccattcagtc caatccaaca catacctatt aagcaactgt tttgtatcca
     4921 ggacaatgcg aagcactgag gtgcctccta ggctgtgcat gtcgcagcct ggcagagagg
     4981 tcaaactcct tcaataacca agaagccacg tgatgatgtg taactactag ggcatcagta
     5041 ggtaaatgtg tctgattgtt ttaaagaata gaaagggttc ttcggggaaa gtttcttggg
     5101 ggagagcaac cttcacatgt cattttggga aaaggaataa aaaatgattg ggacacaaat
     5161 acctcctata ttctcaacct gattttctca aggtgctaaa tttaggaaaa aattcctatt
     5221 tctatatgcc caggtttctg agggaaaact agagagagtc tgaaaatatg ggctgcattc
     5281 actgagcccc tgctaggggc gaggccccgt gctggaggcc ttccacagat ggtctctttt
     5341 atgctgcaca aaagcccagg gagggggtaa agggaaaatc tttgaaaata gaagtgatgc
     5401 ttgcgcaaca ccgtgaatgt actaaacgcc gcaaattgtt ccatttaaaa tgattaattg
     5461 tgtatcatgt gaatttcact tcaataaaaa agaatccagg gaggtagaca tca
//
