LOCUS       NM_002658               2343 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens plasminogen activator, urokinase (PLAU), transcript
            variant 1, mRNA.
ACCESSION   NM_002658
VERSION     NM_002658.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Li,D., Ding,C., Huang,F., Chang,M., Lu,Z., Li,G., Li,Y., Gao,H. and
            Sun,F.
  TITLE     Bioinformatics Analysis of the Expression and Prognostic Value of
            PLAU Gene in Wilms' Tumor
  JOURNAL   Anticancer Res 44 (9), 3829-3842 (2024)
   PUBMED   39197889
  REMARK    GeneRIF: Bioinformatics Analysis of the Expression and Prognostic
            Value of PLAU Gene in Wilms' Tumor.
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Guo,J., Wang,H., Huang,C., Lai,C., Shang,W., Luo,S. and Chen,L.
  TITLE     PLAU, transcriptionally negatively regulated by GATA6, promotes
            lung squamous carcinoma cell proliferation and migration
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1871 (5), 119744 (2024)
   PUBMED   38702016
  REMARK    GeneRIF: PLAU, transcriptionally negatively regulated by GATA6,
            promotes lung squamous carcinoma cell proliferation and migration.
REFERENCE   3  (bases 1 to 2343)
  AUTHORS   Cui,X., Sun,H., Liu,X., Bai,Y., Bai,Y., Cui,Y., Wang,B., Zhang,S.
            and Li,X.
  TITLE     PLAU promotes cell proliferation and migration of head and neck
            cancer via STAT3 signaling pathway
  JOURNAL   Exp Cell Res 438 (2), 114056 (2024)
   PUBMED   38663475
  REMARK    GeneRIF: PLAU promotes cell proliferation and migration of head and
            neck cancer via STAT3 signaling pathway.
REFERENCE   4  (bases 1 to 2343)
  AUTHORS   Chen,X., Zhang,S., Wu,X., Lei,Y., Lei,B. and Zhao,Z.
  TITLE     Inflammatory cytokines and oral lichen planus: a Mendelian
            randomization study
  JOURNAL   Front Immunol 15, 1332317 (2024)
   PUBMED   38390325
  REMARK    GeneRIF: Inflammatory cytokines and oral lichen planus: a Mendelian
            randomization study.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2343)
  AUTHORS   Cetinsoy,O., Anyanwu,I., Krishnanand,H., Natarajan,G.,
            Ramachandran,N., Thomas,A. and Brookes,K.J.
  TITLE     Gene Association Study of the Urokinase Plasminogen Activator and
            Its Receptor Gene in Alzheimer's Disease
  JOURNAL   J Alzheimers Dis 99 (1), 241-250 (2024)
   PUBMED   38669542
  REMARK    GeneRIF: Gene Association Study of the Urokinase Plasminogen
            Activator and Its Receptor Gene in Alzheimer's Disease.
REFERENCE   6  (bases 1 to 2343)
  AUTHORS   Mekkawy,A.H., Pourgholami,M.H. and Morris,D.L.
  TITLE     Involvement of urokinase-type plasminogen activator system in
            cancer: an overview
  JOURNAL   Med Res Rev 34 (5), 918-956 (2014)
   PUBMED   24549574
  REMARK    Review article
REFERENCE   7  (bases 1 to 2343)
  AUTHORS   Naldini,L., Tamagnone,L., Vigna,E., Sachs,M., Hartmann,G.,
            Birchmeier,W., Daikuhara,Y., Tsubouchi,H., Blasi,F. and
            Comoglio,P.M.
  TITLE     Extracellular proteolytic cleavage by urokinase is required for
            activation of hepatocyte growth factor/scatter factor
  JOURNAL   EMBO J 11 (13), 4825-4833 (1992)
   PUBMED   1334458
REFERENCE   8  (bases 1 to 2343)
  AUTHORS   Li,X., Smith,R.A. and Dobson,C.M.
  TITLE     Sequential 1H NMR assignments and secondary structure of the
            kringle domain from urokinase
  JOURNAL   Biochemistry 31 (40), 9562-9571 (1992)
   PUBMED   1327118
REFERENCE   9  (bases 1 to 2343)
  AUTHORS   Stephens,R.W., Aumailley,M., Timpl,R., Reisberg,T., Tapiovaara,H.,
            Myohanen,H., Murphy-Ullrich,J. and Vaheri,A.
  TITLE     Urokinase binding to laminin-nidogen. Structural requirements and
            interactions with heparin
  JOURNAL   Eur J Biochem 207 (3), 937-942 (1992)
   PUBMED   1499567
REFERENCE   10 (bases 1 to 2343)
  AUTHORS   Moroi,M. and Aoki,N.
  TITLE     Isolation and characterization of alpha2-plasmin inhibitor from
            human plasma. A novel proteinase inhibitor which inhibits
            activator-induced clot lysis
  JOURNAL   J Biol Chem 251 (19), 5956-5965 (1976)
   PUBMED   134998
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC349055.1, BC013575.2,
            AL596247.22 and AB208961.1.
            
            On Jan 1, 2020 this sequence version replaced NM_002658.5.
            
            Summary: This gene encodes a secreted serine protease that converts
            plasminogen to plasmin. The encoded preproprotein is
            proteolytically processed to generate A and B polypeptide chains.
            These chains associate via a single disulfide bond to form the
            catalytically inactive high molecular weight urokinase-type
            plasminogen activator (HMW-uPA). HMW-uPA can be further processed
            into the catalytically active low molecular weight urokinase-type
            plasminogen activator (LMW-uPA). This low molecular weight form
            does not bind to the urokinase-type plasminogen activator receptor.
            Mutations in this gene may be associated with Quebec platelet
            disorder and late-onset Alzheimer's disease. Alternative splicing
            results in multiple transcript variants, at least one of which
            encodes an isoform that is proteolytically processed. [provided by
            RefSeq, Jan 2016].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M15476.1, AK312960.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000372764.4/ ENSP00000361850.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-531               DC349055.1         32-562
            532-539             BC013575.2         506-513
            540-540             AL596247.22        62243-62243
            541-1554            BC013575.2         515-1528
            1555-2343           AB208961.1         898-1686
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q22.2"
     gene            1..2343
                     /gene="PLAU"
                     /gene_synonym="ATF; BDPLT5; QPD; u-PA; UPA; URK"
                     /note="plasminogen activator, urokinase"
                     /db_xref="GeneID:5328"
                     /db_xref="HGNC:HGNC:9052"
                     /db_xref="MIM:191840"
     exon            1..87
                     /gene="PLAU"
                     /gene_synonym="ATF; BDPLT5; QPD; u-PA; UPA; URK"
                     /inference="alignment:Splign:2.1.0"
     exon            88..175
                     /gene="PLAU"
                     /gene_synonym="ATF; BDPLT5; QPD; u-PA; UPA; URK"
                     /inference="alignment:Splign:2.1.0"
     CDS             119..1414
                     /gene="PLAU"
                     /gene_synonym="ATF; BDPLT5; QPD; u-PA; UPA; URK"
                     /EC_number="3.4.21.73"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; plasminogen activator, urinary; U-plasminogen
                     activator; urokinase-type plasminogen activator"
                     /codon_start=1
                     /product="urokinase-type plasminogen activator isoform 1
                     preproprotein"
                     /protein_id="NP_002649.2"
                     /db_xref="CCDS:CCDS7339.1"
                     /db_xref="GeneID:5328"
                     /db_xref="HGNC:HGNC:9052"
                     /db_xref="MIM:191840"
                     /translation="MRALLARLLLCVLVVSDSKGSNELHQVPSNCDCLNGGTCVSNKY
                     FSNIHWCNCPKKFGGQHCEIDKSKTCYEGNGHFYRGKASTDTMGRPCLPWNSATVLQQ
                     TYHAHRSDALQLGLGKHNYCRNPDNRRRPWCYVQVGLKLLVQECMVHDCADGKKPSSP
                     PEELKFQCGQKTLRPRFKIIGGEFTTIENQPWFAAIYRRHRGGSVTYVCGGSLISPCW
                     VISATHCFIDYPKKEDYIVYLGRSRLNSNTQGEMKFEVENLILHKDYSADTLAHHNDI
                     ALLKIRSKEGRCAQPSRTIQTICLPSMYNDPQFGTSCEITGFGKENSTDYLYPEQLKM
                     TVVKLISHRECQQPHYYGSEVTTKMLCAADPQWKTDSCQGDSGGPLVCSLQGRMTLTG
                     IVSWGRGCALKDKPGVYTRVSHFLPWIRSHTKEENGLAL"
     exon            176..203
                     /gene="PLAU"
                     /gene_synonym="ATF; BDPLT5; QPD; u-PA; UPA; URK"
                     /inference="alignment:Splign:2.1.0"
     exon            204..311
                     /gene="PLAU"
                     /gene_synonym="ATF; BDPLT5; QPD; u-PA; UPA; URK"
                     /inference="alignment:Splign:2.1.0"
     exon            312..486
                     /gene="PLAU"
                     /gene_synonym="ATF; BDPLT5; QPD; u-PA; UPA; URK"
                     /inference="alignment:Splign:2.1.0"
     exon            487..578
                     /gene="PLAU"
                     /gene_synonym="ATF; BDPLT5; QPD; u-PA; UPA; URK"
                     /inference="alignment:Splign:2.1.0"
     exon            579..798
                     /gene="PLAU"
                     /gene_synonym="ATF; BDPLT5; QPD; u-PA; UPA; URK"
                     /inference="alignment:Splign:2.1.0"
     exon            799..947
                     /gene="PLAU"
                     /gene_synonym="ATF; BDPLT5; QPD; u-PA; UPA; URK"
                     /inference="alignment:Splign:2.1.0"
     exon            948..1088
                     /gene="PLAU"
                     /gene_synonym="ATF; BDPLT5; QPD; u-PA; UPA; URK"
                     /inference="alignment:Splign:2.1.0"
     exon            1089..1237
                     /gene="PLAU"
                     /gene_synonym="ATF; BDPLT5; QPD; u-PA; UPA; URK"
                     /inference="alignment:Splign:2.1.0"
     exon            1238..2343
                     /gene="PLAU"
                     /gene_synonym="ATF; BDPLT5; QPD; u-PA; UPA; URK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2310..2315
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLAU"
                     /gene_synonym="ATF; BDPLT5; QPD; u-PA; UPA; URK"
                     /note="hexamer: AATAAA"
     regulatory      2323..2328
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLAU"
                     /gene_synonym="ATF; BDPLT5; QPD; u-PA; UPA; URK"
                     /note="hexamer: AATAAA"
     polyA_site      2334
                     /gene="PLAU"
                     /gene_synonym="ATF; BDPLT5; QPD; u-PA; UPA; URK"
     polyA_site      2343
                     /gene="PLAU"
                     /gene_synonym="ATF; BDPLT5; QPD; u-PA; UPA; URK"
                     /note="major polyA site"
ORIGIN      
        1 acagtgcgga gaccgcagcc ccggagcccg ggccagggtc cacctgtccc cgcagcgccg
       61 gctcgcgccc tcctgccgca gccaccgagc cgccgtctag cgccccgacc tcgccaccat
      121 gagagccctg ctggcgcgcc tgcttctctg cgtcctggtc gtgagcgact ccaaaggcag
      181 caatgaactt catcaagttc catcgaactg tgactgtcta aatggaggaa catgtgtgtc
      241 caacaagtac ttctccaaca ttcactggtg caactgccca aagaaattcg gagggcagca
      301 ctgtgaaata gataagtcaa aaacctgcta tgaggggaat ggtcactttt accgaggaaa
      361 ggccagcact gacaccatgg gccggccctg cctgccctgg aactctgcca ctgtccttca
      421 gcaaacgtac catgcccaca gatctgatgc tcttcagctg ggcctgggga aacataatta
      481 ctgcaggaac ccagacaacc ggaggcgacc ctggtgctat gtgcaggtgg gcctaaagct
      541 gcttgtccaa gagtgcatgg tgcatgactg cgcagatgga aaaaagccct cctctcctcc
      601 agaagaatta aaatttcagt gtggccaaaa gactctgagg ccccgcttta agattattgg
      661 gggagaattc accaccatcg agaaccagcc ctggtttgcg gccatctaca ggaggcaccg
      721 ggggggctct gtcacctacg tgtgtggagg cagcctcatc agcccttgct gggtgatcag
      781 cgccacacac tgcttcattg attacccaaa gaaggaggac tacatcgtct acctgggtcg
      841 ctcaaggctt aactccaaca cgcaagggga gatgaagttt gaggtggaaa acctcatcct
      901 acacaaggac tacagcgctg acacgcttgc tcaccacaac gacattgcct tgctgaagat
      961 ccgttccaag gagggcaggt gtgcgcagcc atcccggact atacagacca tctgcctgcc
     1021 ctcgatgtat aacgatcccc agtttggcac aagctgtgag atcactggct ttggaaaaga
     1081 gaattctacc gactatctct atccggagca gctgaaaatg actgttgtga agctgatttc
     1141 ccaccgggag tgtcagcagc cccactacta cggctctgaa gtcaccacca aaatgctgtg
     1201 tgctgctgac ccacagtgga aaacagattc ctgccaggga gactcagggg gacccctcgt
     1261 ctgttccctc caaggccgca tgactttgac tggaattgtg agctggggcc gtggatgtgc
     1321 cctgaaggac aagccaggcg tctacacgag agtctcacac ttcttaccct ggatccgcag
     1381 tcacaccaag gaagagaatg gcctggccct ctgagggtcc ccagggagga aacgggcacc
     1441 acccgctttc ttgctggttg tcatttttgc agtagagtca tctccatcag ctgtaagaag
     1501 agactgggaa gataggctct gcacagatgg atttgcctgt gccacccacc agggcgaacg
     1561 acaatagctt taccctcagg cataggcctg ggtgctggct gcccagaccc ctctggccag
     1621 gatggagggg tggtcctgac tcaacatgtt actgaccagc aacttgtctt tttctggact
     1681 gaagcctgca ggagttaaaa agggcagggc atctcctgtg catgggtgaa gggagagcca
     1741 gctcccccga cggtgggcat ttgtgaggcc catggttgag aaatgaataa tttcccaatt
     1801 aggaagtgta acagctgagg tctcttgagg gagcttagcc aatgtgggag cagcggtttg
     1861 gggagcagag acactaacga cttcagggca gggctctgat attccatgaa tgtatcagga
     1921 aatatatatg tgtgtgtatg tttgcacact tgtgtgtggg ctgtgagtgt aagtgtgagt
     1981 aagagctggt gtctgattgt taagtctaaa tatttcctta aactgtgtgg actgtgatgc
     2041 cacacagagt ggtctttctg gagaggttat aggtcactcc tggggcctct tgggtccccc
     2101 acgtgacagt gcctgggaat gtattattct gcagcatgac ctgtgaccag cactgtctca
     2161 gtttcacttt cacatagatg tccctttctt ggccagttat cccttccttt tagcctagtt
     2221 catccaatcc tcactgggtg gggtgaggac cactcctgta cactgaatat ttatatttca
     2281 ctatttttat ttatattttt gtaattttaa ataaaagtga tcaataaaat gtgatttttc
     2341 tga
//
