LOCUS       NM_000930               3062 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens plasminogen activator, tissue type (PLAT), transcript
            variant 1, mRNA.
ACCESSION   NM_000930
VERSION     NM_000930.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Pala,Z.R., Alves E Silva,T.L., Minai,M., Crews,B.,
            Patino-Martinez,E., Carmona-Rivera,C., Valenzuela Leon,P.C.,
            Martin-Martin,I., Flores-Garcia,Y., Cachau,R.E., Muslinkina,L.,
            Gittis,A.G., Srivastava,N., Garboczi,D.N., Alves,D.A., Kaplan,M.J.,
            Fischer,E., Calvo,E. and Vega-Rodriguez,J.
  TITLE     Mosquito salivary apyrase regulates blood meal hemostasis and
            facilitates malaria parasite transmission
  JOURNAL   Nat Commun 15 (1), 8194 (2024)
   PUBMED   39294191
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Yepes,M.
  TITLE     Fibrinolytic and Non-fibrinolytic Roles of Tissue-type Plasminogen
            Activator in the Ischemic Brain
  JOURNAL   Neuroscience 542, 69-80 (2024)
   PUBMED   37574107
  REMARK    GeneRIF: Fibrinolytic and Non-fibrinolytic Roles of Tissue-type
            Plasminogen Activator in the Ischemic Brain.
            Review article
REFERENCE   3  (bases 1 to 3062)
  AUTHORS   Ruikar,K., Khode,V., Shetty,S.S., Sarathkumar,E., Patil,P.,
            Patil,S., Bargale,A., Sadashiv,R. and Shetty,P.
  TITLE     Association of pro-fibrinolytic receptor AnnexinA2 with tissue
            plasminogen activator/Inhibitor-1 in pre-eclampsia
  JOURNAL   Afr Health Sci 23 (1), 309-319 (2023)
   PUBMED   37545924
  REMARK    GeneRIF: Association of pro-fibrinolytic receptor AnnexinA2 with
            tissue plasminogen activator/Inhibitor-1 in pre-eclampsia.
REFERENCE   4  (bases 1 to 3062)
  AUTHORS   Zhang,C., Salamon,J. and Zhang,R.
  TITLE     Correlations between the Changing Levels of Tissue Plasminogen
            Activator and Adiposity Following Exercise-Induced Weight Loss
  JOURNAL   Nutrients 14 (23), 5159 (2022)
   PUBMED   36501190
  REMARK    GeneRIF: Correlations between the Changing Levels of Tissue
            Plasminogen Activator and Adiposity Following Exercise-Induced
            Weight Loss.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3062)
  AUTHORS   Chevilley,A., Lesept,F., Lenoir,S., Ali,C., Parcq,J. and Vivien,D.
  TITLE     Impacts of tissue-type plasminogen activator (tPA) on neuronal
            survival
  JOURNAL   Front Cell Neurosci 9, 415 (2015)
   PUBMED   26528141
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3062)
  AUTHORS   Orth,K., Madison,E.L., Gething,M.J., Sambrook,J.F. and Herz,J.
  TITLE     Complexes of tissue-type plasminogen activator and its serpin
            inhibitor plasminogen-activator inhibitor type 1 are internalized
            by means of the low density lipoprotein receptor-related
            protein/alpha 2-macroglobulin receptor
  JOURNAL   Proc Natl Acad Sci U S A 89 (16), 7422-7426 (1992)
   PUBMED   1502153
REFERENCE   7  (bases 1 to 3062)
  AUTHORS   Downing,A.K., Driscoll,P.C., Harvey,T.S., Dudgeon,T.J., Smith,B.O.,
            Baron,M. and Campbell,I.D.
  TITLE     Solution structure of the fibrin binding finger domain of
            tissue-type plasminogen activator determined by 1H nuclear magnetic
            resonance
  JOURNAL   J Mol Biol 225 (3), 821-833 (1992)
   PUBMED   1602484
REFERENCE   8  (bases 1 to 3062)
  AUTHORS   de Vos,A.M., Ultsch,M.H., Kelley,R.F., Padmanabhan,K., Tulinsky,A.,
            Westbrook,M.L. and Kossiakoff,A.A.
  TITLE     Crystal structure of the kringle 2 domain of tissue plasminogen
            activator at 2.4-A resolution
  JOURNAL   Biochemistry 31 (1), 270-279 (1992)
   PUBMED   1310033
REFERENCE   9  (bases 1 to 3062)
  AUTHORS   Itagaki,Y., Yasuda,H., Morinaga,T., Mitsuda,S. and Higashio,K.
  TITLE     Purification and characterization of tissue plasminogen activator
            secreted by human embryonic lung diploid fibroblasts, IMR-90 cells
  JOURNAL   Agric Biol Chem 55 (5), 1225-1232 (1991)
   PUBMED   1368681
  REMARK    Erratum:[Agric Biol Chem 1991 Jun;55(6):A10]
REFERENCE   10 (bases 1 to 3062)
  AUTHORS   Edlund,T., Ny,T., Ranby,M., Heden,L.O., Palm,G., Holmgren,E. and
            Josephson,S.
  TITLE     Isolation of cDNA sequences coding for a part of human tissue
            plasminogen activator
  JOURNAL   Proc Natl Acad Sci U S A 80 (2), 349-352 (1983)
   PUBMED   6572897
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC424502.1, BC013968.2 and
            DA031108.1.
            
            On May 17, 2019 this sequence version replaced NM_000930.4.
            
            Summary: This gene encodes tissue-type plasminogen activator, a
            secreted serine protease that converts the proenzyme plasminogen to
            plasmin, a fibrinolytic enzyme. The encoded preproprotein is
            proteolytically processed by plasmin or trypsin to generate heavy
            and light chains. These chains associate via disulfide linkages to
            form the heterodimeric enzyme. This enzyme plays a role in cell
            migration and tissue remodeling. Increased enzymatic activity
            causes hyperfibrinolysis, which manifests as excessive bleeding,
            while decreased activity leads to hypofibrinolysis, which can
            result in thrombosis or embolism. Alternative splicing of this gene
            results in multiple transcript variants, at least one of which
            encodes an isoform that is proteolytically processed. [provided by
            RefSeq, Jan 2016].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.164230.1, BC095403.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000220809.9/ ENSP00000220809.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                DC424502.1         160-174
            16-2547             BC013968.2         1-2532
            2548-3062           DA031108.1         1-515               c
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p11.21"
     gene            1..3062
                     /gene="PLAT"
                     /gene_synonym="T-PA; TPA"
                     /note="plasminogen activator, tissue type"
                     /db_xref="GeneID:5327"
                     /db_xref="HGNC:HGNC:9051"
                     /db_xref="MIM:173370"
     exon            1..72
                     /gene="PLAT"
                     /gene_synonym="T-PA; TPA"
                     /inference="alignment:Splign:2.1.0"
     exon            73..170
                     /gene="PLAT"
                     /gene_synonym="T-PA; TPA"
                     /inference="alignment:Splign:2.1.0"
     CDS             99..1787
                     /gene="PLAT"
                     /gene_synonym="T-PA; TPA"
                     /EC_number="3.4.21.68"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; t-plasminogen activator; alteplase; reteplase;
                     plasminogen/activator kringle"
                     /codon_start=1
                     /product="tissue-type plasminogen activator isoform 1
                     preproprotein"
                     /protein_id="NP_000921.1"
                     /db_xref="CCDS:CCDS6126.1"
                     /db_xref="GeneID:5327"
                     /db_xref="HGNC:HGNC:9051"
                     /db_xref="MIM:173370"
                     /translation="MDAMKRGLCCVLLLCGAVFVSPSQEIHARFRRGARSYQVICRDE
                     KTQMIYQQHQSWLRPVLRSNRVEYCWCNSGRAQCHSVPVKSCSEPRCFNGGTCQQALY
                     FSDFVCQCPEGFAGKCCEIDTRATCYEDQGISYRGTWSTAESGAECTNWNSSALAQKP
                     YSGRRPDAIRLGLGNHNYCRNPDRDSKPWCYVFKAGKYSSEFCSTPACSEGNSDCYFG
                     NGSAYRGTHSLTESGASCLPWNSMILIGKVYTAQNPSAQALGLGKHNYCRNPDGDAKP
                     WCHVLKNRRLTWEYCDVPSCSTCGLRQYSQPQFRIKGGLFADIASHPWQAAIFAKHRR
                     SPGERFLCGGILISSCWILSAAHCFQERFPPHHLTVILGRTYRVVPGEEEQKFEVEKY
                     IVHKEFDDDTYDNDIALLQLKSDSSRCAQESSVVRTVCLPPADLQLPDWTECELSGYG
                     KHEALSPFYSERLKEAHVRLYPSSRCTSQHLLNRTVTDNMLCAGDTRSGGPQANLHDA
                     CQGDSGGPLVCLNDGRMTLVGIISWGLGCGQKDVPGVYTKVTNYLDWIRDNMRP"
     exon            171..213
                     /gene="PLAT"
                     /gene_synonym="T-PA; TPA"
                     /inference="alignment:Splign:2.1.0"
     exon            214..351
                     /gene="PLAT"
                     /gene_synonym="T-PA; TPA"
                     /inference="alignment:Splign:2.1.0"
     exon            352..462
                     /gene="PLAT"
                     /gene_synonym="T-PA; TPA"
                     /inference="alignment:Splign:2.1.0"
     exon            463..637
                     /gene="PLAT"
                     /gene_synonym="T-PA; TPA"
                     /inference="alignment:Splign:2.1.0"
     exon            638..729
                     /gene="PLAT"
                     /gene_synonym="T-PA; TPA"
                     /inference="alignment:Splign:2.1.0"
     exon            730..901
                     /gene="PLAT"
                     /gene_synonym="T-PA; TPA"
                     /inference="alignment:Splign:2.1.0"
     exon            902..987
                     /gene="PLAT"
                     /gene_synonym="T-PA; TPA"
                     /inference="alignment:Splign:2.1.0"
     exon            988..1183
                     /gene="PLAT"
                     /gene_synonym="T-PA; TPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1320
                     /gene="PLAT"
                     /gene_synonym="T-PA; TPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1321..1461
                     /gene="PLAT"
                     /gene_synonym="T-PA; TPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1462..1628
                     /gene="PLAT"
                     /gene_synonym="T-PA; TPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1629..3062
                     /gene="PLAT"
                     /gene_synonym="T-PA; TPA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2511..2516
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLAT"
                     /gene_synonym="T-PA; TPA"
                     /note="hexamer: AATAAA"
     polyA_site      2547
                     /gene="PLAT"
                     /gene_synonym="T-PA; TPA"
                     /note="major polyA site"
ORIGIN      
        1 agagctgaga tcctacagga gtccagggct ggagagaaaa cctctgcgag gaaagggaag
       61 gagcaagccg tgaatttaag ggacgctgtg aagcaatcat ggatgcaatg aagagagggc
      121 tctgctgtgt gctgctgctg tgtggagcag tcttcgtttc gcccagccag gaaatccatg
      181 cccgattcag aagaggagcc agatcttacc aagtgatctg cagagatgaa aaaacgcaga
      241 tgatatacca gcaacatcag tcatggctgc gccctgtgct cagaagcaac cgggtggaat
      301 attgctggtg caacagtggc agggcacagt gccactcagt gcctgtcaaa agttgcagcg
      361 agccaaggtg tttcaacggg ggcacctgcc agcaggccct gtacttctca gatttcgtgt
      421 gccagtgccc cgaaggattt gctgggaagt gctgtgaaat agataccagg gccacgtgct
      481 acgaggacca gggcatcagc tacaggggca cgtggagcac agcggagagt ggcgccgagt
      541 gcaccaactg gaacagcagc gcgttggccc agaagcccta cagcgggcgg aggccagacg
      601 ccatcaggct gggcctgggg aaccacaact actgcagaaa cccagatcga gactcaaagc
      661 cctggtgcta cgtctttaag gcggggaagt acagctcaga gttctgcagc acccctgcct
      721 gctctgaggg aaacagtgac tgctactttg ggaatgggtc agcctaccgt ggcacgcaca
      781 gcctcaccga gtcgggtgcc tcctgcctcc cgtggaattc catgatcctg ataggcaagg
      841 tttacacagc acagaacccc agtgcccagg cactgggcct gggcaaacat aattactgcc
      901 ggaatcctga tggggatgcc aagccctggt gccacgtgct gaagaaccgc aggctgacgt
      961 gggagtactg tgatgtgccc tcctgctcca cctgcggcct gagacagtac agccagcctc
     1021 agtttcgcat caaaggaggg ctcttcgccg acatcgcctc ccacccctgg caggctgcca
     1081 tctttgccaa gcacaggagg tcgcccggag agcggttcct gtgcgggggc atactcatca
     1141 gctcctgctg gattctctct gccgcccact gcttccagga gaggtttccg ccccaccacc
     1201 tgacggtgat cttgggcaga acataccggg tggtccctgg cgaggaggag cagaaatttg
     1261 aagtcgaaaa atacattgtc cataaggaat tcgatgatga cacttacgac aatgacattg
     1321 cgctgctgca gctgaaatcg gattcgtccc gctgtgccca ggagagcagc gtggtccgca
     1381 ctgtgtgcct tcccccggcg gacctgcagc tgccggactg gacggagtgt gagctctccg
     1441 gctacggcaa gcatgaggcc ttgtctcctt tctattcgga gcggctgaag gaggctcatg
     1501 tcagactgta cccatccagc cgctgcacat cacaacattt acttaacaga acagtcaccg
     1561 acaacatgct gtgtgctgga gacactcgga gcggcgggcc ccaggcaaac ttgcacgacg
     1621 cctgccaggg cgattcggga ggccccctgg tgtgtctgaa cgatggccgc atgactttgg
     1681 tgggcatcat cagctggggc ctgggctgtg gacagaagga tgtcccgggt gtgtacacca
     1741 aggttaccaa ctacctagac tggattcgtg acaacatgcg accgtgacca ggaacacccg
     1801 actcctcaaa agcaaatgag atcccgcctc ttcttcttca gaagacactg caaaggcgca
     1861 gtgcttctct acagacttct ccagacccac cacaccgcag aagcgggacg agaccctaca
     1921 ggagagggaa gagtgcattt tcccagatac ttcccatttt ggaagttttc aggacttggt
     1981 ctgatttcag gatactctgt cagatgggaa gacatgaatg cacactagcc tctccaggaa
     2041 tgcctcctcc ctgggcagaa agtggccatg ccaccctgtt ttcagctaaa gcccaacctc
     2101 ctgacctgtc accgtgagca gctttggaaa caggaccaca aaaatgaaag catgtctcaa
     2161 tagtaaaaga taacaagatc tttcaggaaa gacggattgc attagaaata gacagtatat
     2221 ttatagtcac aagagcccag cagggcctca aagttggggc aggctggctg gcccgtcatg
     2281 ttcctcaaaa gcacccttga cgtcaagtct ccttcccctt tccccactcc ctggctctca
     2341 gaaggtattc cttttgtgta cagtgtgtaa agtgtaaatc ctttttcttt ataaacttta
     2401 gagtagcatg agagaattgt atcatttgaa caactaggct tcagcatatt tatagcaatc
     2461 catgttagtt tttactttct gttgccacaa ccctgtttta tactgtactt aataaattca
     2521 gatatatttt tcacagtttt tccaaaatca gagtggaatg gttttgttat agatgctgta
     2581 tcccactctt tattcatgtt cacattttaa aatcatttgg aattctgctt cactcgctta
     2641 acatatacac aacacctgta acatacaagg caatgggcta ggtgctccag accgggaaaa
     2701 ggagggacag gaatgcttgg tctgatgggc taatatggca tttagagaag taccaaggta
     2761 cagtggagcc ggtcacaaaa gggcagactt gtagtagaat tcagttgcaa gagggattgg
     2821 ggaatcttaa ggaaaaaata gaatcttaag gaaaaaataa ctgggtgaga cgtggactgt
     2881 ggacaggtgt ggaaaaggca ctctccatgg aggtatgaat atgtagaggg ccaagagagg
     2941 ggagtacagg gagaaatgag ttgagcttgt ctgaagtgaa cttcaggaag aggaacatag
     3001 gctggaattt agattatggg ggctctgaac accaaactga gtttggactt aattgacttc
     3061 tg
//
