LOCUS       NM_001146729            3042 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens phospholipase A and acyltransferase 5 (PLAAT5),
            transcript variant 2, mRNA.
ACCESSION   NM_001146729
VERSION     NM_001146729.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 3042)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   4  (bases 1 to 3042)
  AUTHORS   Uyama,T., Ikematsu,N., Inoue,M., Shinohara,N., Jin,X.H., Tsuboi,K.,
            Tonai,T., Tokumura,A. and Ueda,N.
  TITLE     Generation of N-acylphosphatidylethanolamine by members of the
            phospholipase A/acyltransferase (PLA/AT) family
  JOURNAL   J Biol Chem 287 (38), 31905-31919 (2012)
   PUBMED   22825852
REFERENCE   5  (bases 1 to 3042)
  AUTHORS   Jin,X.H., Uyama,T., Wang,J., Okamoto,Y., Tonai,T. and Ueda,N.
  TITLE     cDNA cloning and characterization of human and mouse
            Ca(2+)-independent phosphatidylethanolamine N-acyltransferases
  JOURNAL   Biochim Biophys Acta 1791 (1), 32-38 (2009)
   PUBMED   19000777
REFERENCE   6  (bases 1 to 3042)
  AUTHORS   Jin,X.H., Okamoto,Y., Morishita,J., Tsuboi,K., Tonai,T. and Ueda,N.
  TITLE     Discovery and characterization of a Ca2+-independent
            phosphatidylethanolamine N-acyltransferase generating the
            anandamide precursor and its congeners
  JOURNAL   J Biol Chem 282 (6), 3614-3623 (2007)
   PUBMED   17158102
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AP001591.5 and AP000484.5.
            
            On Jun 2, 2019 this sequence version replaced NM_001146729.1.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 5' coding region compared to variant 1. This
            results in a shorter protein (isoform 2) compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC034222.1, SRR1803613.231794.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000540857.6/ ENSP00000444809.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-281               AP001591.5         12978-13258         c
            282-372             AP000484.5         104970-105060       c
            373-478             AP000484.5         103598-103703       c
            479-587             AP000484.5         83084-83192         c
            588-850             AP000484.5         80837-81099         c
            851-3042            AP000484.5         76131-78322         c
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q12.3"
     gene            1..3042
                     /gene="PLAAT5"
                     /gene_synonym="HRASLS5; HRLP5; HRSL5; iNAT; PLAAT-5; RLP1"
                     /note="phospholipase A and acyltransferase 5"
                     /db_xref="GeneID:117245"
                     /db_xref="HGNC:HGNC:24978"
                     /db_xref="MIM:611474"
     exon            1..281
                     /gene="PLAAT5"
                     /gene_synonym="HRASLS5; HRLP5; HRSL5; iNAT; PLAAT-5; RLP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             134..943
                     /gene="PLAAT5"
                     /gene_synonym="HRASLS5; HRLP5; HRSL5; iNAT; PLAAT-5; RLP1"
                     /EC_number="3.1.1.4"
                     /EC_number="3.1.1.32"
                     /note="isoform 2 is encoded by transcript variant 2;
                     lecithin-retinol acyltransferase (LRAT)-like protein-1;
                     HRAS-like suppressor 5; H-rev107-like protein 5;
                     calcium-independent phosphatidylethanolamine
                     N-acyltransferase; Ca(2+)-independent N-acyltransferase;
                     testicular tissue protein Li 90; phospholipase
                     A/acyltransferase-5; HRAS like suppressor family member 5"
                     /codon_start=1
                     /product="phospholipase A and acyltransferase 5 isoform 2"
                     /protein_id="NP_001140201.2"
                     /db_xref="CCDS:CCDS53646.1"
                     /db_xref="GeneID:117245"
                     /db_xref="HGNC:HGNC:24978"
                     /db_xref="MIM:611474"
                     /translation="MGLSPGAEGEYALRLPRIPPPLPKPASRTASTGPKDQPPALRRS
                     AVPHSEESVGFAALVQLPAKQPPPGTLEQGRSIQQGEKAVVSLETTPSQKADWSSIPK
                     PENEGKLIKQAAEGKPRPRPGDLIEIFRIGYEHWAIYVEDDCVVHLAPPSEEFEVGSI
                     TSIFSNRAVVKYSRLEDVLHGCSWKVNNKLDGTYLPLPVDKIIQRTKKMVNKIVQYSL
                     IEGNCEHFVNGLRYGVPRSQQVEHALMEGAKAAGAVISAVVDSIKPKPITA"
     exon            282..372
                     /gene="PLAAT5"
                     /gene_synonym="HRASLS5; HRLP5; HRSL5; iNAT; PLAAT-5; RLP1"
                     /inference="alignment:Splign:2.1.0"
     exon            373..478
                     /gene="PLAAT5"
                     /gene_synonym="HRASLS5; HRLP5; HRSL5; iNAT; PLAAT-5; RLP1"
                     /inference="alignment:Splign:2.1.0"
     exon            479..587
                     /gene="PLAAT5"
                     /gene_synonym="HRASLS5; HRLP5; HRSL5; iNAT; PLAAT-5; RLP1"
                     /inference="alignment:Splign:2.1.0"
     exon            588..850
                     /gene="PLAAT5"
                     /gene_synonym="HRASLS5; HRLP5; HRSL5; iNAT; PLAAT-5; RLP1"
                     /inference="alignment:Splign:2.1.0"
     exon            851..3042
                     /gene="PLAAT5"
                     /gene_synonym="HRASLS5; HRLP5; HRSL5; iNAT; PLAAT-5; RLP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3022..3027
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLAAT5"
                     /gene_synonym="HRASLS5; HRLP5; HRSL5; iNAT; PLAAT-5; RLP1"
                     /note="hexamer: ATTAAA"
     polyA_site      3042
                     /gene="PLAAT5"
                     /gene_synonym="HRASLS5; HRLP5; HRSL5; iNAT; PLAAT-5; RLP1"
                     /note="major polyA site"
ORIGIN      
        1 gcggttcctc cccgagccgc ccccagtgcc caagcttccg cggctccgcc gaccaggggc
       61 cgcgccgact gggaactcgc caggggcgta gtcccctgca aggcctgggg gccggcgagg
      121 ccgcagaggc gggatgggcc tgagcccggg cgccgagggg gagtacgcgc tccgcctccc
      181 taggattccc ccacccctcc ccaaacccgc ctcgcgaacc gccagtaccg ggcccaagga
      241 ccagccgcct gcgctcagac gttcagctgt gccccactca gaagaatccg tgggattcgc
      301 agcgttggtc cagctcccag ccaagcagcc tccgccgggc acattagaac agggcagaag
      361 catccagcaa ggggagaagg ctgtagttag cttggagacc acacccagcc agaaagcaga
      421 ctggagttca attccaaagc ctgagaatga aggcaagtta ataaagcaag cagctgaggg
      481 aaaaccaaga cccagacctg gagacctgat tgagattttt cgaattggct atgagcactg
      541 ggccatctat gtagaagatg attgcgtggt ccatctggct cccccaagtg aggagtttga
      601 ggtgggcagc attacttcca tctttagcaa tcgggccgtg gtgaaataca gtcgtctgga
      661 ggatgtgctg catggctgct cctggaaggt caataacaag ctagatggga cgtacctgcc
      721 cttgccggtg gacaagatca tccagcgtac aaaaaagatg gtcaacaaga tcgtgcagta
      781 cagcctgatt gaagggaact gtgagcactt tgtcaatggc ctcagatatg gcgtaccccg
      841 gagccagcag gtagagcacg ccctgatgga aggagcgaag gctgctggag cagttatttc
      901 agctgtagtg gatagcataa agcccaaacc aataactgcc tgaaggtgat gaaaactcca
      961 gctagaggaa gagttactga cacaagcaaa aagaacatgc ttccttccct tgccttctct
     1021 caatggctcc gattaagaaa attgtgaact tctggaagaa tctacaatgt atccaattac
     1081 ccttccagaa atacatccaa tatatacgag catagaccca cagactctca ggatgggaag
     1141 gatctggtat ctagtgtgta tctcttatgc gccaggcact gtgctaggca tttcatatac
     1201 ggatctaatt tcactctctt tgtaggatgg gaccttgctt gtgtcttagg ctgaccttgg
     1261 tctccgatgc ttgagtgcca tcagaaacat cccagaaacc tctgcttgaa tatttccagt
     1321 aacgaagatg tctttttcca gatgttcttt ttatttaact aaaatgtctc tagcttcttt
     1381 ccatttatga taatcctgcc cttttcgacc cacagtgtag ctcagaggag gaggacctta
     1441 agaaaaactg agattcgtta ttcagggccc atggtacctt aatctagaaa cacatatccc
     1501 aagaaatatt ttctttttaa aattatacat caactttatg ccttattgtt taaaaatctt
     1561 ctatcaaaag tatcatcgat aagacaattt atcttgcttt tgtacctcat aattacccta
     1621 aaatgtgaag tttctagtct cactgatttc agatgaatat ttaaaataat ttgataagtt
     1681 taatcaattg aactctactg tgtgagtgag ggagagaaag gagttatttt tgtgttttga
     1741 agaaagttga caggatggaa gatctgaaat tatggctggg gattgtagtg atagctggta
     1801 gctgaaacca cccaaaaaag ctagaaaagt gttctcttat gcctccagag atacctagaa
     1861 aatatgaaga atgtggaaaa gagcaagatg gaaccctcag agtgtttcag ataaggtctg
     1921 ttggtatctc cataagcttt tctcctattt ggagaaaata gactctcact gaaactcttc
     1981 aatgttttta ccctccatcc ttttcttagc aatctaaact caatggcctc ctgttcccca
     2041 acactgcagt ctgggcatct gactttgtct tccctctgca ctcatttctg tgaatcatga
     2101 ccaggatgaa actgatatcc ctagttcctc ttccccattt cttctcacac tcccctgcct
     2161 gagaatcagt tgagttccca atacctcttc cttcagtctc tattcattat atggtctagg
     2221 ttgagacttc cttggaagca gaacctgagt accaggaact catattttat aaagtatatt
     2281 tattcctttt agctgcaaac taacccagcc atgttttagt gaatgcttta atctgaagat
     2341 gactttgagc tcattgacca tattaatctt tcatcaaaca aactggatgt tgtttcatta
     2401 acaaaaaact cctccacacc aaggagtgtt tagtaacttc tgcacagagg actcatccat
     2461 ctcttgctta tgtttgggac agaaagacac ttctctggct ttttactggc ttgaacccaa
     2521 tgggacagag actctctaga aagtttcagg gctcacattc acttgtttgt ccaaaaaata
     2581 tttttgagtg tctcccaagt gccaaaccct ggctactccc acctttgcct gagcccatga
     2641 gcggtagtct gggggctgga tgtgaggagt tctgagttgg ctaggaagac acaaggcaca
     2701 atggacatgg ggcaaagtcc acttgctgga cattggtccc ttccacttcc aagctgtcca
     2761 tcaggctgag agatctggaa tgcatacccc acactttgaa gatgaggaag ggcgagtttc
     2821 tcaaagtgtg tgtgtgtgtt tctcttttaa tctgtaatgg tttctgcatt ttgaggtaga
     2881 aacagaaatc tcttttttga gtgggaccac tcgggtgcgg cttcttagtc ttgttgatat
     2941 aataaagcac atgcccactc ctcatggagt ggagaggaga aaattctaag ggaacgtact
     3001 ctgaatgcaa atgtttctga gattaaacac tagtcacttg tt
//
