LOCUS       NM_007366              14376 bp    mRNA    linear   PRI 15-OCT-2024
DEFINITION  Homo sapiens phospholipase A2 receptor 1 (PLA2R1), transcript
            variant 1, mRNA.
ACCESSION   NM_007366
VERSION     NM_007366.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 14376)
  AUTHORS   Dolla,G., Nicolas,S., Dos Santos,L.R., Bourgeois,A.,
            Pardossi-Piquard,R., Bihl,F., Zaghrini,C., Justino,J., Payre,C.,
            Mansuelle,P., Garbers,C., Ronco,P., Checler,F., Lambeau,G. and
            Petit-Paitel,A.
  TITLE     Ectodomain shedding of PLA2R1 is mediated by the metalloproteases
            ADAM10 and ADAM17
  JOURNAL   J Biol Chem 300 (7), 107480 (2024)
   PUBMED   38897568
  REMARK    GeneRIF: Ectodomain shedding of PLA2R1 is mediated by the
            metalloproteases ADAM10 and ADAM17.
REFERENCE   2  (bases 1 to 14376)
  AUTHORS   Shao,C., Ye,J., Dong,Z., Liao,B., Feng,S., Hu,S. and Zhong,B.
  TITLE     Phospholipid metabolism-related genotypes of PLA2R1 and CERS4
            contribute to nonobese MASLD
  JOURNAL   Hepatol Commun 8 (6), e0388 (2024)
   PUBMED   38836837
  REMARK    GeneRIF: Phospholipid metabolism-related genotypes of PLA2R1 and
            CERS4 contribute to nonobese MASLD.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 14376)
  AUTHORS   Khan,S., Zuccato,J.A., Ignatchenko,V., Singh,O., Govindarajan,M.,
            Waas,M., Mejia-Guerrero,S., Gao,A., Zadeh,G. and Kislinger,T.
  TITLE     Organelle resolved proteomics uncovers PLA2R1 as a novel cell
            surface marker required for chordoma growth
  JOURNAL   Acta Neuropathol Commun 12 (1), 39 (2024)
   PUBMED   38454495
  REMARK    GeneRIF: Organelle resolved proteomics uncovers PLA2R1 as a novel
            cell surface marker required for chordoma growth.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 14376)
  AUTHORS   McDonnell,T., Wu,H.H.L., Sinha,S. and Chinnadurai,R.
  TITLE     The Role of PLA2R in Primary Membranous Nephropathy: Do We Still
            Need a Kidney Biopsy?
  JOURNAL   Genes (Basel) 14 (7), 1343 (2023)
   PUBMED   37510247
  REMARK    GeneRIF: The Role of PLA2R in Primary Membranous Nephropathy: Do We
            Still Need a Kidney Biopsy?
            Review article
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 14376)
  AUTHORS   Kim,S., Chin,H.J., Na,K.Y., Kim,S., Oh,J., Chung,W., Noh,J.W.,
            Lee,Y.K., Cho,J.T., Lee,E.K. and Chae,D.W.
  CONSRTM   Progressive Renal Disease and Medical Informatics and Genomics
            Research (PREMIER) members
  TITLE     Single nucleotide polymorphisms in the phospholipase A2 receptor
            gene are associated with genetic susceptibility to idiopathic
            membranous nephropathy
  JOURNAL   Nephron Clin Pract 117 (3), c253-c258 (2011)
   PUBMED   20805699
  REMARK    GeneRIF: Single nucleotide polymorphisms in subjects' PLA2R gene
            are associated with the risk of developing idiopathic membranous
            nephropathy.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   6  (bases 1 to 14376)
  AUTHORS   Wistow,G., Bernstein,S.L., Wyatt,M.K., Ray,S., Behal,A.,
            Touchman,J.W., Bouffard,G., Smith,D. and Peterson,K.
  TITLE     Expressed sequence tag analysis of human retina for the NEIBank
            Project: retbindin, an abundant, novel retinal cDNA and alternative
            splicing of other retina-preferred gene transcripts
  JOURNAL   Mol Vis 8, 196-204 (2002)
   PUBMED   12107411
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 14376)
  AUTHORS   Yokota,Y., Higashino,K., Nakano,K., Arita,H. and Hanasaki,K.
  TITLE     Identification of group X secretory phospholipase A(2) as a natural
            ligand for mouse phospholipase A(2) receptor
  JOURNAL   FEBS Lett 478 (1-2), 187-191 (2000)
   PUBMED   10922494
REFERENCE   8  (bases 1 to 14376)
  AUTHORS   Yokota,Y., Ikeda,M., Higashino,K., Nakano,K., Fujii,N., Arita,H.
            and Hanasaki,K.
  TITLE     Enhanced tissue expression and elevated circulating level of
            phospholipase A(2) receptor during murine endotoxic shock
  JOURNAL   Arch Biochem Biophys 379 (1), 7-17 (2000)
   PUBMED   10864436
REFERENCE   9  (bases 1 to 14376)
  AUTHORS   Ancian,P., Lambeau,G., Mattei,M.G. and Lazdunski,M.
  TITLE     The human 180-kDa receptor for secretory phospholipases A2.
            Molecular cloning, identification of a secreted soluble form,
            expression, and chromosomal localization
  JOURNAL   J Biol Chem 270 (15), 8963-8970 (1995)
   PUBMED   7721806
REFERENCE   10 (bases 1 to 14376)
  AUTHORS   Higashino,K., Ishizaki,J., Kishino,J., Ohara,O. and Arita,H.
  TITLE     Structural comparison of phospholipase-A2-binding regions in
            phospholipase-A2 receptors from various mammals
  JOURNAL   Eur J Biochem 225 (1), 375-382 (1994)
   PUBMED   7925459
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP351340.1, AC080166.6,
            BC140823.1, U17033.1 and AC093873.2.
            
            On Nov 22, 2018 this sequence version replaced NM_007366.4.
            
            Summary: This gene represents a phospholipase A2 receptor. The
            encoded protein likely exists as both a transmembrane form and a
            soluble form. The transmembrane receptor may play a role in
            clearance of phospholipase A2, thereby inhibiting its action.
            Polymorphisms at this locus have been associated with
            susceptibility to idiopathic membranous nephropathy. Alternatively
            spliced transcript variants encoding different isoforms have been
            identified.[provided by RefSeq, Sep 2010].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U17033.1, BC140823.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000283243.13/ ENSP00000283243.7
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-85                BP351340.1         1-85
            86-86               AC080166.6         96006-96006         c
            87-473              BP351340.1         86-472
            474-4001            BC140823.1         284-3811
            4002-5018           U17033.1           4002-5018
            5019-5584           AC093873.2         86278-86843         c
            5585-5633           U17033.1           5584-5632
            5634-14376          AC093873.2         77486-86228         c
FEATURES             Location/Qualifiers
     source          1..14376
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q24.2"
     gene            1..14376
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /note="phospholipase A2 receptor 1"
                     /db_xref="GeneID:22925"
                     /db_xref="HGNC:HGNC:9042"
                     /db_xref="MIM:604939"
     exon            1..321
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     CDS             213..4604
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; secretory phospholipase A2 receptor; M-type
                     receptor; C-type lectin domain family 13 member C; 180 kDa
                     secretory phospholipase A2 receptor; phospholipase A2
                     receptor 1, 180kD; phospholipase A2 receptor 1, 180kDa"
                     /codon_start=1
                     /product="secretory phospholipase A2 receptor isoform 1
                     precursor"
                     /protein_id="NP_031392.3"
                     /db_xref="CCDS:CCDS33309.1"
                     /db_xref="GeneID:22925"
                     /db_xref="HGNC:HGNC:9042"
                     /db_xref="MIM:604939"
                     /translation="MLLSPSLLLLLLLGAPRGCAEGVAAALTPERLLEWQDKGIFVIQ
                     SESLKKCIQAGKSVLTLENCKQANKHMLWKWVSNHGLFNIGGSGCLGLNFSAPEQPLS
                     LYECDSTLVSLRWRCNRKMITGPLQYSVQVAHDNTVVASRKYIHKWISYGSGGGDICE
                     YLHKDLHTIKGNTHGMPCMFPFQYNHQWHHECTREGREDDLLWCATTSRYERDEKWGF
                     CPDPTSAEVGCDTIWEKDLNSHICYQFNLLSSLSWSEAHSSCQMQGGTLLSITDETEE
                     NFIREHMSSKTVEVWMGLNQLDEHAGWQWSDGTPLNYLNWSPEVNFEPFVEDHCGTFS
                     SFMPSAWRSRDCESTLPYICKKYLNHIDHEIVEKDAWKYYATHCEPGWNPYNRNCYKL
                     QKEEKTWHEALRSCQADNSALIDITSLAEVEFLVTLLGDENASETWIGLSSNKIPVSF
                     EWSNDSSVIFTNWHTLEPHIFPNRSQLCVSAEQSEGHWKVKNCEERLFYICKKAGHVL
                     SDAESGCQEGWERHGGFCYKIDTVLRSFDQASSGYYCPPALVTITNRFEQAFITSLIS
                     SVVKMKDSYFWIALQDQNDTGEYTWKPVGQKPEPVQYTHWNTHQPRYSGGCVAMRGRH
                     PLGRWEVKHCRHFKAMSLCKQPVENQEKAEYEERWPFHPCYLDWESEPGLASCFKVFH
                     SEKVLMKRTWREAEAFCEEFGAHLASFAHIEEENFVNELLHSKFNWTEERQFWIGFNK
                     RNPLNAGSWEWSDRTPVVSSFLDNTYFGEDARNCAVYKANKTLLPLHCGSKREWICKI
                     PRDVKPKIPFWYQYDVPWLFYQDAEYLFHTFASEWLNFEFVCSWLHSDLLTIHSAHEQ
                     EFIHSKIKALSKYGASWWIGLQEERANDEFRWRDGTPVIYQNWDTGRERTVNNQSQRC
                     GFISSITGLWGSEECSVSMPSICKRKKVWLIEKKKDTPKQHGTCPKGWLYFNYKCLLL
                     NIPKDPSSWKNWTHAQHFCAEEGGTLVAIESEVEQAFITMNLFGQTTSVWIGLQNDDY
                     ETWLNGKPVVYSNWSPFDIINIPSHNTTEVQKHIPLCALLSSNPNFHFTGKWYFEDCG
                     KEGYGFVCEKMQDTSGHGVNTSDMYPMPNTLEYGNRTYKIINANMTWYAAIKTCLMHK
                     AQLVSITDQYHQSFLTVVLNRLGYAHWIGLFTTDNGLNFDWSDGTKSSFTFWKDEESS
                     LLGDCVFADSNGRWHSTACESFLQGAICHVPPETRQSEHPELCSETSIPWIKFKSNCY
                     SFSTVLDSMSFEAAHEFCKKEGSNLLTIKDEAENAFLLEELFAFGSSVQMVWLNAQFD
                     GNNETIKWFDGTPTDQSNWGIRKPDTDYFKPHHCVALRIPEGLWQLSPCQEKKGFICK
                     MEADIHTAEALPEKGPSHSIIPLAVVLTLIVIVAICTLSFCIYKHNGGFFRRLAGFRN
                     PYYPATNFSTVYLEENILISDLEKSDQ"
     exon            322..705
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            706..879
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            880..1053
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1167
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            1168..1311
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            1312..1506
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            1507..1664
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            1665..1763
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            1764..1876
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            1877..2046
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            2047..2249
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            2250..2395
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            2396..2480
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            2481..2613
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            2614..2649
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            2650..2807
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            2808..2872
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            2873..2976
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            2977..3116
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            3117..3234
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            3235..3365
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            3366..3513
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            3514..3752
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            3753..3921
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            3922..4062
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            4063..4179
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            4180..4356
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            4357..4389
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     exon            4390..14376
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8511..8516
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /note="hexamer: AATAAA"
     polyA_site      8531
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /note="major polyA site"
     regulatory      13539..13544
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /note="hexamer: AATATA"
     polyA_site      13556
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /note="major polyA site"
     regulatory      14347..14352
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /note="hexamer: AGTAAA"
     polyA_site      14376
                     /gene="PLA2R1"
                     /gene_synonym="CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R"
                     /note="major polyA site"
ORIGIN      
        1 agagccccga gtgtcggttc actgtggaga cagcggtggc ggagtgggtc tccagggctc
       61 tgggctggca aggcccccgg agggggtggg gcgcggagga ggctacggat ccgcttccgc
      121 gcggcggggc cgggtgcttg ggacgcggct ctgggctccc gggataaggg gctcccggga
      181 caaggggctc ccggagagcc cagtggttag cgatgctgct gtcgccgtcg ctgctgctgc
      241 tgctgctgct gggggcgccg cggggctgcg ccgagggtgt ggcggcggcg cttacccccg
      301 agcggctcct ggagtggcag gataaaggaa tatttgttat ccaaagtgag agtctcaaga
      361 aatgcattca agcaggtaaa tcggttctga ccctggagaa ctgcaagcaa gcaaacaagc
      421 acatgctgtg gaaatgggtt tcaaaccatg gcctctttaa cataggaggc agtggttgcc
      481 tgggcctgaa tttctccgcc ccagagcagc cattaagctt atatgaatgt gactccaccc
      541 tcgtttcctt acggtggcgc tgtaacagga agatgatcac aggcccgctg cagtactctg
      601 tccaggtggc gcatgacaac acagtggtgg cctcacggaa gtatattcat aagtggattt
      661 cttatgggtc aggtggtgga gacatttgtg aatatctaca caaagatttg catacaatca
      721 aagggaacac ccacgggatg ccgtgtatgt ttcccttcca gtataaccat cagtggcatc
      781 atgaatgtac ccgtgaaggt cgggaagatg acttactgtg gtgtgccacg acaagccgtt
      841 atgaaagaga tgaaaagtgg ggattttgcc ctgatcccac ctctgcagaa gtaggttgtg
      901 atactatttg ggagaaggac ctcaattcac acatttgcta ccagttcaac ctgctttcat
      961 ctctctcttg gagtgaggca cattcttcat gccagatgca aggaggtacg ctgttaagta
     1021 ttacagatga aactgaagaa aatttcataa gggagcacat gagcagtaaa acagtggagg
     1081 tgtggatggg cctcaatcag ctggatgaac acgctggctg gcagtggtct gatggaacgc
     1141 cgctcaacta tctgaattgg agcccagagg taaattttga gccatttgtt gaagatcact
     1201 gtggaacatt tagttcattt atgccaagtg cctggaggag tcgggattgt gagtccacct
     1261 tgccatatat atgtaaaaaa tatctaaacc acattgatca tgaaatagtt gaaaaagatg
     1321 cgtggaaata ttatgctacc cactgtgagc ctggctggaa tccctacaat cgtaattgct
     1381 acaaacttca gaaagaagaa aagacctggc atgaggctct gcgttcttgt caggctgata
     1441 acagtgcatt aatagacata acctcattag cagaggtgga gtttcttgta accctccttg
     1501 gagatgaaaa tgcatcagaa acatggattg gtttgagcag caataaaatt ccagtttcct
     1561 ttgaatggtc taatgactct tcagtcatct ttactaattg gcacacactt gagccccaca
     1621 tttttccaaa tagaagccag ctgtgtgtct cagcagagca gtctgaggga cactggaaag
     1681 tcaaaaattg tgaagaaaga cttttttaca tttgtaaaaa agcaggccat gtcctctctg
     1741 atgctgaatc aggatgtcaa gagggatggg agagacatgg tggattctgt tacaaaattg
     1801 acacagtcct tcgaagcttt gaccaagctt ccagcggtta ttactgtcct cctgcacttg
     1861 taaccattac aaacaggttt gaacaggctt ttattaccag tttgatcagt agtgtggtaa
     1921 aaatgaagga cagttatttt tggatagctc ttcaggacca aaatgatacg ggagaataca
     1981 cttggaagcc agtagggcag aaacccgagc cggtgcagta cacacactgg aacacacacc
     2041 agccgcgcta cagtggtggc tgtgttgcca tgcgaggaag gcatccactt ggtcgctggg
     2101 aagtgaagca ctgtcggcac tttaaggcaa tgtccttgtg caagcagcca gttgaaaatc
     2161 aggaaaaagc agagtatgaa gagagatggc cctttcaccc ctgctatttg gactgggagt
     2221 cagagcctgg tctggccagt tgcttcaagg tatttcatag tgaaaaagtt ctgatgaaaa
     2281 gaacatggag agaagctgaa gcattttgcg aagaatttgg agctcatctt gcaagctttg
     2341 cccatattga ggaagagaat tttgtgaatg agctcttaca ttcaaaattt aattggacag
     2401 aagaaaggca gttctggatt ggatttaata aaagaaaccc actgaatgcc ggctcatggg
     2461 agtggtctga tagaactcct gttgtctctt cgtttttaga caacacttat tttggagaag
     2521 atgcaagaaa ctgtgctgtt tataaggcaa acaaaacatt gctgccctta cactgtggtt
     2581 ccaaacgtga atggatatgc aaaatcccaa gagatgtgaa acccaagatt ccgttctggt
     2641 accagtacga tgtaccctgg ctcttttatc aggatgcaga ataccttttt catacctttg
     2701 cctcagaatg gttgaacttt gagtttgtct gtagctggct gcacagtgat cttctcacaa
     2761 ttcattctgc acatgagcaa gaattcatcc acagcaaaat aaaagcgcta tcaaagtatg
     2821 gtgcaagttg gtggattgga cttcaagaag aaagagccaa tgatgaattt cgctggagag
     2881 atggaacacc agtgatatac cagaactggg acacaggaag agaaagaact gtgaataatc
     2941 agagccagag atgtggcttt atttcttcta taacaggact ctggggtagt gaagagtgtt
     3001 cagtttctat gcctagtatc tgtaagcgaa aaaaggtttg gctcatagag aaaaagaaag
     3061 atacaccaaa acaacatgga acgtgtccca aaggatggct atattttaac tataagtgcc
     3121 ttctgctgaa tatccccaaa gacccaagca gttggaagaa ctggacgcat gctcaacatt
     3181 tctgtgctga agaagggggg accctggtcg ccattgaaag tgaggtggag caagctttca
     3241 ttactatgaa tctttttggc cagaccacca gtgtgtggat aggtttacaa aatgatgatt
     3301 atgaaacatg gctaaatgga aagcctgtgg tatattctaa ctggtctcca tttgatataa
     3361 taaatattcc aagtcacaat accactgaag ttcagaaaca cattcctctc tgtgccttac
     3421 tctcaagtaa tcctaatttt catttcactg gaaaatggta ttttgaagac tgtggaaagg
     3481 aaggctatgg gtttgtttgt gaaaaaatgc aagatacttc tggacacggt gtaaatacat
     3541 ctgatatgta tccaatgccc aataccttag aatatggaaa cagaacttac aaaataatta
     3601 atgcaaatat gacttggtat gcagcaataa aaacctgcct gatgcacaaa gcacaactgg
     3661 tcagcatcac agaccagtat caccagtcct tcctcactgt tgtcctcaac cggctaggat
     3721 atgcccactg gattggactg ttcaccacag ataatggtct taattttgac tggtctgatg
     3781 gcaccaaatc ttctttcact ttttggaaag atgaggagtc ctccctcctt ggtgactgcg
     3841 tttttgccga cagcaacgga cgctggcata gcacagcctg cgagtcattt ctgcaaggtg
     3901 ccatttgtca tgtgccacct gaaacaagac aatctgaaca cccagagttg tgctcagaaa
     3961 catctattcc ctggataaaa tttaaaagta attgctacag tttttctaca gtcctagaca
     4021 gtatgagttt tgaggctgct catgaatttt gcaaaaagga aggttctaat cttttaacaa
     4081 tcaaggatga ggctgaaaat gcatttctcc tagaagagct gtttgctttt ggttcttctg
     4141 tccagatggt ttggttgaat gctcaatttg atggtaacaa tgaaaccata aagtggtttg
     4201 atggaactcc cacagaccag tcaaactggg gcattcggaa gccagacaca gactacttca
     4261 agccccatca ttgtgttgcc ttgaggatcc ctgaaggatt atggcagcta tccccgtgtc
     4321 aagaaaaaaa aggctttata tgtaaaatgg aggcagatat tcacactgca gaggcgctgc
     4381 cagaaaaagg accaagtcac agcatcattc ctcttgcggt tgtactgaca ctgatagtca
     4441 ttgtggccat ttgcacactt tccttctgca tatacaagca taacggtggc ttcttcagga
     4501 gacttgcagg gtttcggaat ccttactatc ctgcaaccaa ctttagtaca gtatatttag
     4561 aagaaaatat tctcatttct gatcttgaga agagtgacca ataataatga ggtcagagaa
     4621 tgccacagac accagggtaa gtaaagaaga ctaaacagga gtctcatctg tctttccctt
     4681 tacagcacag atgccattag aatgtgaatt gggtcactat tttaattatt cttgaagtga
     4741 ttactggttt tgaatcttaa ccaaatcaga tgggttttga tttattcatt tccctaaact
     4801 gtgatccatt cttaaaaggg gtaaattatg cattggttat ttttcagaaa gacaagaact
     4861 attaaaagaa actccctatt gaaaactctg aaatcaatgc gaataatagt ttttgcatta
     4921 atgtatctct actaaaattt gggggaattt taaaactaat ctggtatcta ttcagacatt
     4981 tacctgcact cgtaccatta agaaagacag aaagaagcaa aaaaaaatta atcttgtata
     5041 tgagaggaaa aggaaagggc ttctgagagg attcttagtt gtttcttttg aattcacttt
     5101 taatagcagg atttggaaaa tactaatttc tgtgcttaag ggtcacaggt tctgggctct
     5161 caactgatat ttaaggtgac attcattttt attaggtcta acatctcaag ctaaaggaga
     5221 aagaaaaata ccttctttta aatggcaaag accttcatta gcagcacact tttataaaca
     5281 accatatagt taaaatgtgg ccttaaactt tcaattacta aatgatgatt aagttggata
     5341 ttttaaaatg tcttatacat aagcttaaag taatatattg aaactttaac agttgtactg
     5401 ttaaaactca tggactttct gaaaaactaa atatataaaa taatatgtaa cactcttaat
     5461 aactggaaga tataaaatgt aacttggatt taaaggagaa gagctaaaga tctgaattat
     5521 agtctcatta gaaccagaca gatgttgttg agaagtacta aataaattag taatctagat
     5581 atctttatta tgtaaatgag tttaggtgtt ctatttaata aactattttc tgggaacgat
     5641 ctccttttat attagtctag ttagtgtaga tggtaaataa tgattatgat actgtctgta
     5701 aataaaaaat tacagatttt attttccttt attagttcct tataaagaaa acagcttcat
     5761 aatcaatatc taaaaattta ctcattacat aactgtttat tgaattttat caagaaaaaa
     5821 gtgtactgag catggtggca cgcacctgta gtcccagcta cttaggaggc tgagggaaga
     5881 ggatcaactg agcccagaag ttgaaggctg cagtgagcta tggttgcacc actgcactcc
     5941 tgcctgggca actgaatgag accccacctc aaaaaagtgc tgttctagaa acagaggtac
     6001 taaggtgagc aacttggggt aagggctatc ttttcatgcc acttattttc taatgaggga
     6061 aaattgacag taactcagta aagaaatgca tatgcataaa atagcaagtg gttatgaaaa
     6121 tgaagtaagg ttccatgata gagagaaact gggggactct cagggcctcc ctgagagtgt
     6181 gcattttaat tattacaacc aactgcctca aaaccagtgg ccactaatca caagggggag
     6241 ctctgagaga aagtatgaat gaatttctaa gaggcaaacc atcatcatta gaaatacaat
     6301 ttgaagagca taccactaag aatggaatcc ttgtggcctg caacagttaa tccagcttcc
     6361 aaaagcagct ctttgctact gttataataa tttacacact gggacagtct acctccataa
     6421 tttttttaag tattgggact tcatttttta aaagatatta tattatgtgg cagttgcata
     6481 tttatgactc aaaaaataat tggcttgaac tcaccagatg gtataaaagc ccaatttaac
     6541 catcttttga gtggaattaa agttctgttc ttttcaatgg acttggtaaa aactgctaat
     6601 caaccagcct tccagtcaag gtatatagtc acacataata atttgacaaa atacaccatt
     6661 aggaaagtga ttttgcagaa agaagctgta ttgcttctca gtgctccata ctttgatgtt
     6721 tagtattgaa caacatctcc agataatctg gacccatgga cagatcacac acttcttgcc
     6781 attactgaat ttgcagaaat agtactgaag tattctgcta catttttata tttgcatttt
     6841 ttttgaaact ttttttcatt tttttttttt ttttttggag atggagtctt gctctgttgc
     6901 caggcaggag tacagtggca tgatctcagc tcactgcaac cttcaactgc ctggttcgag
     6961 caagtttcct gcctcagcct cccgagtagc tgtgattaca ggcacatgcc accatgccca
     7021 gctaattttt gtatttttag tagagacagg gtttcaccat gttgtccagg atggtctcga
     7081 tctcctgatc tcgtgatcca cccgccccag cctcccaaag tgctaagatt acaggcatga
     7141 gccaccgcac ctggcctcat ctgtattttt aatgtgaact agattttata gctataattc
     7201 gaaaagaaaa aaaagtagca ggtaatgggt atattataca ctatttgcgt gatggttaca
     7261 ccagacttta ccactacata ctgtatttat gtaacaaaat tacacttgta ctccttacat
     7321 ttatgccaaa aaagcggcag gatcacaaat tcacacatag ttaatgtgcc cctccaacag
     7381 tcaattatgt tctatgacta acctgaggat ctttatagaa tctgagtaat ggaaatgcct
     7441 gggtttttcc atttaacaat atattgggct cttacagtta caacagtctt ctttcgggtt
     7501 aaatatttct tctcttgaag ggtccactcc ttattaaaga taagattgaa ctgaatgttt
     7561 acttaaacgt tcagtctttt ggagtttctt tactcttctc aaaatggtgg ttggatatat
     7621 aagaacttgc tgtgtcctgg tagtgttgag gggagaaggt gacaacttac tgtggatgta
     7681 tgttagctgg tgtccactga gaatagctgt ctggcctcac tcctgggaat tctgctggca
     7741 tctgctgtga agtctgcagt tgctttaatc tgtagtctgt tctctttgat ttggcctcat
     7801 tctggctgtc cctggtgctg tcttatctct tgagaactgg ctgtgtctcc tgttgaattc
     7861 cctatccgaa cactgcagtt cttggctaca aggcagtcac ttcacccctg cagtggtgag
     7921 atcccattag ctaccattag ctactctttt cgtccagaaa ggcgaaatgc catagcaaac
     7981 aatcccacag tcttggtgga ttaacacagc aaaggtttat ttctcaccca cacctgcatg
     8041 ggctggcaag aggactttgc tctgttcagt cattcatggg tccatgcttc tcctatatag
     8101 ccagccctct gtctcctaag gcctggagtc ctccatgggc ctccaatctg tggctgacag
     8161 aggatgttga cttgagaccc atctttgatt cattatggag cttccacagg ctgttgaaat
     8221 cctctaagtc aaaagtcctc aaactgggga cacatgccta aggaatacag gagcatgggt
     8281 atttaagaaa cttaatttcc agaatcttca tgtatatttt ttcttaaaac tgatttacct
     8341 gagagcaccc ctggttcttc tttcccacct ctctttacac aaatgccttt ctctaccttt
     8401 aaaaagaaaa agaaaaagaa atggctttca tttcagatct tactatggga aaatcctcca
     8461 tggctccaaa gaaaaatatt cgaagtattg atgtcagtga agcttctttt aataaagaaa
     8521 ttgtaaactc attctgtaga tcctcacgtc tttctctgtg ttaggacatt tttctgttaa
     8581 agttgaagcg atgacttaga aatgagttat tttgccccat gttcatattc tgaattaata
     8641 tgtattatac agtggaacag ctggagtgct actttcattt gaaaatttat ctcatccact
     8701 gcctgactat tgtcagagaa caagctgtag aaaaagtctg gtgaaagcaa agtgaagcat
     8761 gtgttgccaa gaacctaaca tcttatgtta tccagaagaa agtcccaagg cttgatttat
     8821 cttagatttc tatgtgagtt gtgaggatga gtattaacaa gcttatttga actgaaaaat
     8881 gaattcagac cttttctgaa gtttttttta tttggctcat tggtttgaca acaaggattg
     8941 actttaccca ttaagtttta tgatgaacat tttgtattaa ttgaatgaac taaatctgct
     9001 gctcttgtgt tctgattaaa gcttgtaata gggtaaaggc attttaatag aaaaatagta
     9061 ttgactgaac tataatgaag tgatatttta cttttcccag ccttcatttg tatatcaggt
     9121 aaagcaagat gcctgtaagt gaaagagtaa caggcataat gaacagttat ttgaagagtc
     9181 ttagtaaagc cttttaattt tacttgtagg aaattgacat gaatcattct aatgactggg
     9241 taacaaatca ctttgcaagt agggtgtttc cacttctatg ctttaaaaaa aactgaagga
     9301 gaacataatc aagcagttgg ttgatataga aaataatttt taatgtagaa tcactgattt
     9361 tttggcacct atgagaaagg aattcaagta atagagggat attgttataa caaacctcct
     9421 tttattccca tctacttatt tatatgaaca aagtttctat gttaagataa gttagagagt
     9481 gctgtggaaa caaaccctgt aatactagtg gttaaaaaca aaactcagaa gttaatttat
     9541 tgatcatgcg aagcctgcta tggtcaggac acttgcctcc aagcagtaac tcacagatcc
     9601 aggctgtgtc ctacctccag aacatggggc ttccagtgct gctatggcac tagagaactg
     9661 ttaagttccc aggtttagga agaaacacgt ggcacttcca ctcgcgtgtc attggctgga
     9721 actagtcaca tgacctaacc taactgaaag gaaatccagg aagtcttctg ctttgtgtgc
     9781 tcaggaaaga gaggataagg gactacagac agcaccagca attgctacca gagtttctca
     9841 acatgtatag ctacaaaaaa agaagagaga gaacagaatt gatgctgaac cttgatttat
     9901 actagcagtt tttaatacta attaattttt atctatgata tatccatttc attaacagat
     9961 ccatttgcta tcattttagt ttgacatata ctatcttaat ctttgtaaca tatttatttt
    10021 gttctgatca attgtatact aacaataatt gaaatgacaa ctcaatatag aagaaaatgt
    10081 gtaatactta gagcctatgt tcacagggac ttcttaaaaa ataattttag gctgggcgcg
    10141 gtggctcacg cctgtaatcc cagcaacttt gggaggccga ggcgggtgga tcacgaggtg
    10201 aggagatcga gaccatcctg gctaacaagg tgaaaccccg tctctactaa aaatacaaaa
    10261 aaattagctg tgtgttgcgg cgggtgcctg tattcccagc tactcgggag gctgaggcag
    10321 gagaatggca tgaacctgga agtcggagct tacagtgagc cgagatggcg ccactgcact
    10381 ccagcctggg cgacagagcg agactctctc tcaaaaaaaa aaaaaaaaaa aaaaaattaa
    10441 tttatataca tatttttact gcaggaaaat atgacaaggt aatgaataaa atatttcaca
    10501 aattaaagta tatctcatta ggataaaatt ctgtggggga gttggaatgg aaatacaaga
    10561 ttgaaagaaa gtaatatctc tgactgctga agaggttatt tctattttta aatagattat
    10621 gatagaattc aaattgttgt agtatctcca gtcaaaggaa cttaggcttc ttgagaaagt
    10681 ggttgattgc aggtctaagg caggaaaata caagatgaaa ctggaatatc ttgtgatggc
    10741 agaaaataag gaggtgctca caaaaagatg gcacatgtct gaaggctaca ggaaccaacc
    10801 tgaaggagct ctccagtgga aatgctggaa caatttgagc aaacaataaa aaattataat
    10861 attggattat aacccacaca ataaatatct gtgtgtccat actgatgtaa acaattgaat
    10921 gagtgaatga atgaatgaaa agaagaaaca gttcttctcc ccagtaaatt ccaattgata
    10981 aatgtagaag aaatgggaga aacaaaaatc actatcagga aaacacaaca gtaataattg
    11041 ttggcaagac ctaccagttg gtgcaaaagt tagtgggtga atgtttgagg aaaaacagaa
    11101 tatttgcata accttagagt attttcccca aaataatcac taattacaaa gagaaagtaa
    11161 taactttaca gtagagaaac ttctttaatc aagggatcaa ggttaacaga agcagtaata
    11221 agccacatca gcatcatgta ccccctggta tggcattgag aagggcacat gacttctttg
    11281 gtatcctccc caaaatgcat aatctcaatc atgaaaaaac attagacaaa tccaagttga
    11341 gggacattcc acaagataaa tgatcacttc aaaagtgtcc tggtcatgaa agataaggaa
    11401 agattgagga aatgtcaagg attggaggac actaaagaga catgacaact acgtgcaagt
    11461 tgaaatcctc aaatggatca tggaacagaa aatggtcatt atgggaaaat gggaaagtaa
    11521 aataaagtct atggtttaat taatagtatt gtaccagtat tagtttcttt gttttgatga
    11581 ttataccatg gttttatgag atgtaccatt agaagaagct gaaggaggct gtacaggaac
    11641 tctctggact aagttggcaa tttttctgta aacctaaaat catttcaata taaaaagtta
    11701 acattttgct atgatattag acaccactgg atatattaaa agggtgataa ggcactttta
    11761 tttttacatg ttgttattta taatattctg aaaattgcat cctaattata aaaaattttg
    11821 gctgtcaact tagaaattcg tgaagcaata tgccttcttt catgattctt ttaacaattt
    11881 tgtatgctag ccaaaaagtt tgaggaccac tgctatatga cattttttct ctcatgtctc
    11941 agatgagaag cagggcaaaa ccctgtgtgt ctccaactta cctaacacac catccgctgt
    12001 aggattagga gccagacacg tcttgctgca ggccatgctc acttccatct gctctctttt
    12061 ccctgcattc ttcttttgtg tcacttccct gacttgtcag tacttctaat tctcatagtc
    12121 ctgctatccc ctacaacctc gtgccttagt attaataggg tacagcaggg aaaaactcac
    12181 ttagtttcca ttttgttctt ggagaagaaa aatttggaga catttccaaa atggcatgta
    12241 catcataatt cttattgcca acatttataa gaaactttga ctgtagtgat tatcaagata
    12301 catgcttgga atatttattc cctctttccc cacctttcaa aataaggatt tagagcagga
    12361 gttggcaggg ccaaatccag cctgcagcct attttggaaa taaagttatt gggatgctgc
    12421 catccccatt cactcacata ctgtgcatga ctgctttcat gcttccatga cagagctgag
    12481 tggttgcaac agagaccgaa tggcctgcaa agcctaaaat atttactttc tggaccctta
    12541 caggaagttt gccaacccct gatttagaaa gtgaaaacct aaatggggaa gtcttacata
    12601 ctttaacgta tcttagcaag cactgttatg ttaatgtata tggacagcac ttaaaatata
    12661 tcaataaaca attatgatca gtaaacattt atttagagcc tattgtggcc cagagagtgt
    12721 cctgtgctca gaggctataa ataccaataa caccccatct ctcccctcat ggaactcagt
    12781 ctcaggggag aggaatatgt aaatgataag tgcaatgcaa tgtgacataa ataatatcaa
    12841 gaagaagaga atcagaaaaa aaaggagagg ggactgatta attctgtcag aagtagagaa
    12901 aggcttaaca agataagtga tatgtattaa attgtaggaa atgttagata aacagctttc
    12961 tttagtattt tttgagttag aaagcaagtt tttagcttgg ggtcagaata tttttagaga
    13021 aaaagtttaa aatgtgccta aatctgaaat tctggattta aaaaagtgaa aatctgcatg
    13081 ttcctctaat acctaatgac gaattctaat tgcagaagga tgccaataac taaaaccaca
    13141 aaatgaaccc tatgtatacc ggtgaccttc tttactcaga attaattaac acaagcactt
    13201 gtttcttgca gagcactttg ctgtaattta gaacaaatta agggaaaaaa tcattcaagt
    13261 gctgttttct tcacaccatg aagtaagcat gatggaccaa agttaactaa tttaaaatat
    13321 tatcatccat ttcttgtaat tatgaatagc aataccatct tttttcttca aaaaaaaaaa
    13381 aagacatcat gatctatgct attttcaact tgtttcctgt gcttctatta atggaaattg
    13441 atttttcaag attaatgtaa aatgtgtttc tgtttctccc attaagtctc ttatttatat
    13501 tagatccaat gtatatgttc ctggatattt ttcaaataaa tatattttta atataataca
    13561 gcaaattgta aattgttaaa ggaccattta taatgttttc tccttgccct ccctagcact
    13621 gaaactgtgg ttttattatt gtcttcacac tgttgtttgt gaattaactt gagctccttg
    13681 agttcctact tctaagagat tatttaaact ttacttttta ccttcttagt atacagagct
    13741 tggtaggagc tggaaacccg actcacagat ttaggaaaca gtgggttctt gtccctgtgg
    13801 acatcagaac ttgggggacg gagagataga gaaccagaag gcagaacaag gaaaaactca
    13861 aagaaaggaa tggggaggaa tggaaacgta acaggacatg ttgcctcagt gagtcaccaa
    13921 gggctttgga gttcttagcc caaaatgtgg ttctgtggag gctgaggaca aacttcagga
    13981 cagagcatgc cttaatcgat gtgctaagcc actggctcgg gcagtcctta agcaagggct
    14041 cctctgatca gacaaggaat tgtttccttc ccttttactc cagatgacac ctaaaggtgc
    14101 aaaagaaaac catctctgca cactaccaaa cagacagtgc gtgaacgaag cagaccctcc
    14161 tgaccacagc caaggactgg agagcttggt gaaactggtg ctcaaggtag aaattccctg
    14221 cgattgctac tgcaacttat actaaacgtc ccaagtgaag tgaagggaga aaaaagagat
    14281 attaagcaag aaggcccact tgtttatgca ttgctttaga agataatctg taattgcata
    14341 ttaataagta aagttaacgt tacacttaaa taataa
//
