LOCUS       NM_015715               2600 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens phospholipase A2 group III (PLA2G3), mRNA.
ACCESSION   NM_015715
VERSION     NM_015715.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ray,U., Roy,D., Jin,L., Thirusangu,P., Staub,J., Xiao,Y.,
            Kalogera,E., Wahner Hendrickson,A.E., Cullen,G.D., Goergen,K.,
            Oberg,A.L. and Shridhar,V.
  TITLE     Group III phospholipase A2 downregulation attenuated survival and
            metastasis in ovarian cancer and promotes chemo-sensitization
  JOURNAL   J Exp Clin Cancer Res 40 (1), 182 (2021)
   PUBMED   34082797
  REMARK    GeneRIF: Group III phospholipase A2 downregulation attenuated
            survival and metastasis in ovarian cancer and promotes
            chemo-sensitization.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Murase,R., Taketomi,Y., Miki,Y., Nishito,Y., Saito,M., Fukami,K.,
            Yamamoto,K. and Murakami,M.
  TITLE     Group III phospholipase A2 promotes colitis and colorectal cancer
  JOURNAL   Sci Rep 7 (1), 12261 (2017)
   PUBMED   28947740
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2600)
  AUTHORS   Ewing,H., Fernandez-Vega,V., Spicer,T.P., Chase,P., Brown,S.,
            Scampavia,L., Roush,W.R., Riley,S., Rosen,H., Hodder,P., Lambeau,G.
            and Gelb,M.H.
  TITLE     Fluorometric High-Throughput Screening Assay for Secreted
            Phospholipases A2 Using Phospholipid Vesicles
  JOURNAL   J Biomol Screen 21 (7), 713-721 (2016)
   PUBMED   27146384
  REMARK    GeneRIF: Several hits were discovered, and data have been uploaded
            to PubChem. This study describes the first high-throughput optical
            screening assay for secreted phospholipase A2 inhibitors based on a
            phospholipid vesicle substrate
REFERENCE   4  (bases 1 to 2600)
  AUTHORS   Yui,D., Nishida,Y., Nishina,T., Mogushi,K., Tajiri,M.,
            Ishibashi,S., Ajioka,I., Ishikawa,K., Mizusawa,H., Murayama,S. and
            Yokota,T.
  TITLE     Enhanced Phospholipase A2 Group 3 Expression by Oxidative Stress
            Decreases the Insulin-Degrading Enzyme
  JOURNAL   PLoS One 10 (12), e0143518 (2015)
   PUBMED   26637123
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2600)
  AUTHORS   Kazama,S., Kitayama,J., Hiyoshi,M., Taketomi,Y., Murakami,M.,
            Nishikawa,T., Tanaka,T., Tanaka,J., Kiyomatsu,T., Kawai,K.,
            Hata,K., Yamaguchi,H., Nozawa,H., Ishihara,S., Sunami,E. and
            Watanabe,T.
  TITLE     Phospholipase A2 Group III and Group X Have Opposing Associations
            with Prognosis in Colorectal Cancer
  JOURNAL   Anticancer Res 35 (5), 2983-2990 (2015)
   PUBMED   25964585
  REMARK    GeneRIF: sPLA2GIII expression may be used as a risk factor for
            lymph node metastasis and a prognostic marker in colorectal cancer.
            In addition, sPLA2GIII and sPLA2GX may play opposing roles in
            colorectal carcinogenesis
REFERENCE   6  (bases 1 to 2600)
  AUTHORS   Masuda,S., Yamamoto,K., Hirabayashi,T., Ishikawa,Y., Ishii,T.,
            Kudo,I. and Murakami,M.
  TITLE     Human group III secreted phospholipase A2 promotes neuronal
            outgrowth and survival
  JOURNAL   Biochem J 409 (2), 429-438 (2008)
   PUBMED   17868035
  REMARK    GeneRIF: potential contribution of sPLA2-III to neuronal
            differentiation and its function under certain conditions
REFERENCE   7  (bases 1 to 2600)
  AUTHORS   Murakami,M., Masuda,S., Shimbara,S., Ishikawa,Y., Ishii,T. and
            Kudo,I.
  TITLE     Cellular distribution, post-translational modification, and
            tumorigenic potential of human group III secreted phospholipase
            A(2)
  JOURNAL   J Biol Chem 280 (26), 24987-24998 (2005)
   PUBMED   15863501
  REMARK    GeneRIF: sPLA(2)-III may have a role in cancer development by
            stimulating tumor cell growth and angiogenesis
REFERENCE   8  (bases 1 to 2600)
  AUTHORS   Murakami,M., Masuda,S., Shimbara,S., Bezzine,S., Lazdunski,M.,
            Lambeau,G., Gelb,M.H., Matsukura,S., Kokubu,F., Adachi,M. and
            Kudo,I.
  TITLE     Cellular arachidonate-releasing function of novel classes of
            secretory phospholipase A2s (groups III and XII)
  JOURNAL   J Biol Chem 278 (12), 10657-10667 (2003)
   PUBMED   12522102
  REMARK    GeneRIF: cellular arachidonate (AA) release and prostaglandin (PG)
            production are regulated by novel classes of secretory
            phospholipase A(2)s (sPLA(2)s), groups III and XII
REFERENCE   9  (bases 1 to 2600)
  AUTHORS   Chowdhury,B., Mantile-Selvaggi,G., Miele,L., Cordella-Miele,E.,
            Zhang,Z. and Mukherjee,A.B.
  TITLE     Lys 43 and Asp 46 in alpha-helix 3 of uteroglobin are essential for
            its phospholipase A2 inhibitory activity
  JOURNAL   Biochem Biophys Res Commun 295 (4), 877-883 (2002)
   PUBMED   12127976
  REMARK    GeneRIF: Lys 43 and Asp 46 in rhUG are critical residues for the
            sPLA2-inhibitory activity of UG
REFERENCE   10 (bases 1 to 2600)
  AUTHORS   Valentin,E., Ghomashchi,F., Gelb,M.H., Lazdunski,M. and Lambeau,G.
  TITLE     Novel human secreted phospholipase A(2) with homology to the group
            III bee venom enzyme
  JOURNAL   J Biol Chem 275 (11), 7492-7496 (2000)
   PUBMED   10713052
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF220490.1, AC005005.1 and
            BC025316.1.
            
            On Nov 22, 2018 this sequence version replaced NM_015715.4.
            
            Summary: This gene encodes a protein that belongs to the secreted
            phospholipase A2 family, whose members include the bee venom
            enzyme. The encoded enzyme functions in lipid metabolism and
            catalyzes the calcium-dependent hydrolysis of the sn-2 acyl bond of
            phospholipids to release arachidonic acid and lysophospholipids.
            This enzyme acts as a negative regulator of ciliogenesis, and may
            play a role in cancer development by stimulating tumor cell growth
            and angiogenesis. This gene is associated with oxidative stress,
            and polymorphisms in this gene are linked to risk for Alzheimer's
            disease. [provided by RefSeq, Apr 2014].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC025316.1, AF220490.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2144333, SAMEA2145893
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000215885.4/ ENSP00000215885.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-361               AF220490.1         100-460
            362-362             AC005005.1         37962-37962
            363-2598            AF220490.1         462-2697
            2599-2600           BC025316.1         2574-2575
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q12.2"
     gene            1..2600
                     /gene="PLA2G3"
                     /gene_synonym="GIII-SPLA2; sPLA2-III; SPLA2III"
                     /note="phospholipase A2 group III"
                     /db_xref="GeneID:50487"
                     /db_xref="HGNC:HGNC:17934"
                     /db_xref="MIM:611651"
     exon            1..668
                     /gene="PLA2G3"
                     /gene_synonym="GIII-SPLA2; sPLA2-III; SPLA2III"
                     /inference="alignment:Splign:2.1.0"
     CDS             155..1684
                     /gene="PLA2G3"
                     /gene_synonym="GIII-SPLA2; sPLA2-III; SPLA2III"
                     /EC_number="3.1.1.4"
                     /note="group III secreted phospholipase A2;
                     phosphatidylcholine 2-acylhydrolase GIII; group 3
                     secretory phospholipase A2; phosphatidylcholine
                     2-acylhydrolase 3"
                     /codon_start=1
                     /product="group 3 secretory phospholipase A2 precursor"
                     /protein_id="NP_056530.2"
                     /db_xref="CCDS:CCDS13889.1"
                     /db_xref="GeneID:50487"
                     /db_xref="HGNC:HGNC:17934"
                     /db_xref="MIM:611651"
                     /translation="MGVQAGLFGMLGFLGVALGGSPALRWYRTSCHLTKAVPGNPLGY
                     LSFLAKDAQGLALIHARWDAHRRLQSCSWEDEPELTAAYGALCAHETAWGSFIHTPGP
                     ELQRALATLQSQWEACRALEESPAGARKKRAAGQSGVPGGGHQREKRGWTMPGTLWCG
                     VGDSAGNSSELGVFQGPDLCCREHDRCPQNISPLQYNYGIRNYRFHTISHCDCDTRFQ
                     QCLQNQHDSISDIVGVAFFNVLEIPCFVLEEQEACVAWYWWGGCRMYGTVPLARLQPR
                     TFYNASWSSRATSPTPSSRSPAPPKPRQKQHLRKGPPHQKGSKRPSKANTTALQDPMV
                     SPRLDVAPTGLQGPQGGLKPQGARWVCRSFRRHLDQCEHQIGPREIEFQLLNSAQEPL
                     FHCNCTRRLARFLRLHSPPEVTNMLWELLGTTCFKLAPPLDCVEGKNCSRDPRAIRVS
                     ARHLRRLQQRRHQLQDKGTDERQPWPSEPLRGPMSFYNQCLQLTQAARRPDRQQKSWS
                     Q"
     exon            669..801
                     /gene="PLA2G3"
                     /gene_synonym="GIII-SPLA2; sPLA2-III; SPLA2III"
                     /inference="alignment:Splign:2.1.0"
     exon            802..936
                     /gene="PLA2G3"
                     /gene_synonym="GIII-SPLA2; sPLA2-III; SPLA2III"
                     /inference="alignment:Splign:2.1.0"
     exon            937..1220
                     /gene="PLA2G3"
                     /gene_synonym="GIII-SPLA2; sPLA2-III; SPLA2III"
                     /inference="alignment:Splign:2.1.0"
     exon            1221..1353
                     /gene="PLA2G3"
                     /gene_synonym="GIII-SPLA2; sPLA2-III; SPLA2III"
                     /inference="alignment:Splign:2.1.0"
     exon            1354..1470
                     /gene="PLA2G3"
                     /gene_synonym="GIII-SPLA2; sPLA2-III; SPLA2III"
                     /inference="alignment:Splign:2.1.0"
     exon            1471..2600
                     /gene="PLA2G3"
                     /gene_synonym="GIII-SPLA2; sPLA2-III; SPLA2III"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2574..2579
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLA2G3"
                     /gene_synonym="GIII-SPLA2; sPLA2-III; SPLA2III"
                     /note="hexamer: ATTAAA"
     polyA_site      2600
                     /gene="PLA2G3"
                     /gene_synonym="GIII-SPLA2; sPLA2-III; SPLA2III"
                     /note="major polyA site"
ORIGIN      
        1 ctctttcctc ctcctcccgc agctccattc attggtcccg ccgcaccggg cctgctgggc
       61 tccgcttccg ttccactgct cagctgccgc ctggtggggc caccaagggc aggcatccca
      121 ggggctttgt ctgactggac tgggccagtg cagaatgggg gttcaggcag ggctgtttgg
      181 gatgctgggc ttcctggggg tggccctggg gggctcccct gccctccgct ggtacaggac
      241 ctcctgccac ttgaccaagg ccgtccctgg caacccactg gggtacctga gcttcctggc
      301 caaggatgct cagggactgg ccctgatcca tgcccgctgg gatgcgcata ggaggctgca
      361 gtcatgtagc tgggaggatg agccggagct caccgcagcc tacggtgctc tctgtgctca
      421 tgagactgcc tggggctcct tcatccacac ccccggaccc gagctgcaga gagcactggc
      481 cactcttcag agtcagtggg aggcatgccg agcgcttgag gagagtccag caggggccag
      541 gaagaagcga gcagcagggc agagtggagt ccctggtgga gggcaccagc gagagaagag
      601 aggatggacc atgcctggca cactgtggtg tggagttgga gattctgctg ggaactcctc
      661 ggagctgggg gtcttccagg gacctgatct ctgttgccgg gaacatgacc gctgcccaca
      721 gaacatctca cccttgcagt acaactatgg catccgaaac taccgattcc acaccatctc
      781 ccactgtgac tgtgacacca ggtttcagca atgcctacag aatcagcacg actccatctc
      841 ggacatcgtg ggcgtggcct tcttcaacgt gctggagatc ccctgctttg tgctggagga
      901 gcaggaggcg tgtgtggcgt ggtactggtg gggcgggtgt aggatgtacg gcacagtgcc
      961 cctcgctcgc ctgcagccca ggaccttcta caatgcctcc tggagctccc gggccacctc
     1021 cccaactccc agctcccgga gcccagcccc tcccaagcct cgacagaagc agcaccttcg
     1081 gaaggggcca ccacatcaga aagggtccaa gcgccccagc aaagccaaca ccacagccct
     1141 ccaggaccct atggtctctc ccaggcttga tgtggccccc acaggcctcc agggcccaca
     1201 gggtggccta aaacctcagg gtgcccgctg ggtctgccgc agcttccgcc gccacctgga
     1261 ccagtgtgag caccagattg ggccccggga aatcgagttc cagctgctca acagcgccca
     1321 agagcccctc ttccactgca actgcacgcg ccgtctggca cgcttcctga ggctccacag
     1381 cccacccgag gttaccaaca tgctttggga gctgctgggc acaacctgct tcaagctggc
     1441 ccctccactg gactgtgtgg aaggcaaaaa ctgttccaga gaccctaggg ccatcagggt
     1501 gtcagcccgg cacttgcgga ggcttcagca gaggcgacac cagctccagg ataaaggcac
     1561 agatgagagg cagccatggc cttcagagcc cctgagaggc cccatgtcat tctacaacca
     1621 gtgcctgcag ctaacccagg cagccaggag acccgacagg cagcagaagt cctggagcca
     1681 gtgacctcag tttcagcttt cctgggcacc agcctggacc ttgcccatgg ctatgccaag
     1741 ccttgggaat ctcagcctcc cctccgtagg ttagactgaa gcatggcaga ggctgttgtg
     1801 gacaatcaag aggatgaatg gggggatctc aaggcccaaa tgctggacca catctcctgc
     1861 tgttctgggt aaccttgagc tatgtatgac acaactcttc tatgcctgga tgtggtgttc
     1921 aggaagctca ttctgatgcc ctgggctttg gccttgccag ggaacttcac atacagatga
     1981 gaatggggaa agggtaactt attgcagcag ccccaggcag taccaggagg aggtacatgt
     2041 atgtccgtgt tgcaaaaata atacatgcct caaaaacctg cctaggggag ccctagtgcc
     2101 tgggtgctgt ggcctgaggt agcaggtggg aagttaggga tgtcacagaa atgtctgtgt
     2161 ctgaatccag gattggggtg ggtgttggag agggctttca gctcccctcc tcccaggggg
     2221 gcctcttttt ttaacggctg ccgtgccctt cctggcccag ccctaaacct aaattcaaat
     2281 ctcctccatg cctttgcgca aaggacctcc ctcttgcact ctaagcctta gtttcctcct
     2341 ctaaaaaaag ggggtctcta aacaggagct acctcatagg gttgttgagg attaagtgaa
     2401 ccaatacata tacagtgctt agcacttaat aagtattccc ccctgcgaca cctagctgaa
     2461 ctatggtttg gtgtctgatc ttgagaggtt gatgtaacct tttaaaggcc tcagttcgct
     2521 cacctgtgaa atgggtctaa gaatagcact gatctcacag ggttgtgatg cagattaaag
     2581 gagatggcat gtgtaatgta
//
