LOCUS       NM_012320               2694 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens phospholipase A2 group XV (PLA2G15), transcript
            variant 1, mRNA.
ACCESSION   NM_012320
VERSION     NM_012320.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Navarrete,M., Wilkins,J.A., Chan,F.V., Ye,B., Nickerson,P. and
            Ho,J.
  TITLE     Phospholipase A2 group XV activity during cardiopulmonary bypass
            surgery
  JOURNAL   Clin Biochem 88, 49-55 (2021)
   PUBMED   33307060
  REMARK    GeneRIF: Phospholipase A2 group XV activity during cardiopulmonary
            bypass surgery.
REFERENCE   3  (bases 1 to 2694)
  AUTHORS   Bouley,R.A., Hinkovska-Galcheva,V., Shayman,J.A. and Tesmer,J.J.G.
  TITLE     Structural Basis of Lysosomal Phospholipase A2 Inhibition by Zn2
  JOURNAL   Biochemistry 58 (13), 1709-1717 (2019)
   PUBMED   30830753
  REMARK    GeneRIF: Zn(2+) was found to inhibit the esterase activity of LPLA2
            in a noncompetitive manner exclusively at a neutral pH (between 6.5
            and 8.0). Because lysosomes are reservoirs of Zn(2+) in cells, the
            pH optimum of LPLA2 might allow it to catalyze acyl transfer
            unimpeded within the organelle.
REFERENCE   4  (bases 1 to 2694)
  AUTHORS   Hinkovska-Galcheva,V., Kelly,R., Manthei,K.A., Bouley,R., Yuan,W.,
            Schwendeman,A., Tesmer,J.J.G. and Shayman,J.A.
  TITLE     Determinants of pH profile and acyl chain selectivity in lysosomal
            phospholipase A2
  JOURNAL   J Lipid Res 59 (7), 1205-1218 (2018)
   PUBMED   29724779
REFERENCE   5  (bases 1 to 2694)
  AUTHORS   Bond,J., Tran Quang,C., Hypolite,G., Belhocine,M., Bergon,A.,
            Cordonnier,G., Ghysdael,J., Macintyre,E., Boissel,N., Spicuglia,S.
            and Asnafi,V.
  TITLE     Novel Intergenically Spliced Chimera, NFATC3-PLA2G15, Is Associated
            with Aggressive T-ALL Biology and Outcome
  JOURNAL   Mol Cancer Res 16 (3), 470-475 (2018)
   PUBMED   29330284
  REMARK    GeneRIF: Expression of the NFATC3-PLA2G15 chimera correlated with
            aggressive disease biology in murine patient-derived T-ALL
            xenografts, and poor prognosis in human T-ALL patients.
REFERENCE   6  (bases 1 to 2694)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   7  (bases 1 to 2694)
  AUTHORS   Hiraoka,M., Abe,A. and Shayman,J.A.
  TITLE     Cloning and characterization of a lysosomal phospholipase A2,
            1-O-acylceramide synthase
  JOURNAL   J Biol Chem 277 (12), 10090-10099 (2002)
   PUBMED   11790796
REFERENCE   8  (bases 1 to 2694)
  AUTHORS   Wang,A. and Dennis,E.A.
  TITLE     Mammalian lysophospholipases
  JOURNAL   Biochim Biophys Acta 1439 (1), 1-16 (1999)
   PUBMED   10395961
  REMARK    Review article
REFERENCE   9  (bases 1 to 2694)
  AUTHORS   Taniyama,Y., Shibata,S., Kita,S., Horikoshi,K., Fuse,H.,
            Shirafuji,H., Sumino,Y. and Fujino,M.
  TITLE     Cloning and expression of a novel lysophospholipase which
            structurally resembles lecithin cholesterol acyltransferase
  JOURNAL   Biochem Biophys Res Commun 257 (1), 50-56 (1999)
   PUBMED   10092508
REFERENCE   10 (bases 1 to 2694)
  AUTHORS   Ohta,T., Michel,J.J., Schottelius,A.J. and Xiong,Y.
  TITLE     ROC1, a homolog of APC11, represents a family of cullin partners
            with an associated ubiquitin ligase activity
  JOURNAL   Mol Cell 3 (4), 535-541 (1999)
   PUBMED   10230407
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK074828.1 and BC062605.1.
            
            On Nov 23, 2018 this sequence version replaced NM_012320.3.
            
            Summary: Lysophospholipases are enzymes that act on biological
            membranes to regulate the multifunctional lysophospholipids. The
            protein encoded by this gene hydrolyzes lysophosphatidylcholine to
            glycerophosphorylcholine and a free fatty acid. This enzyme is
            present in the plasma and thought to be associated with
            high-density lipoprotein. A later paper contradicts the function of
            this gene. It demonstrates that this gene encodes a lysosomal
            enzyme instead of a lysophospholipase and has both
            calcium-independent phospholipase A2 and transacylase activities.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant represents the longer transcript
            and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.7879.1, AK074828.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000219345.10/ ENSP00000219345.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1160              AK074828.1         30-1189
            1161-2530           BC062605.1         1154-2523
            2531-2691           AK074828.1         2560-2720
            2692-2694           BC062605.1         2685-2687
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q22.1"
     gene            1..2694
                     /gene="PLA2G15"
                     /gene_synonym="ACS; GXVPLA2; LLPL; LPLA2; LYPLA3"
                     /note="phospholipase A2 group XV"
                     /db_xref="GeneID:23659"
                     /db_xref="HGNC:HGNC:17163"
                     /db_xref="MIM:609362"
     exon            1..181
                     /gene="PLA2G15"
                     /gene_synonym="ACS; GXVPLA2; LLPL; LPLA2; LYPLA3"
                     /inference="alignment:Splign:2.1.0"
     CDS             55..1293
                     /gene="PLA2G15"
                     /gene_synonym="ACS; GXVPLA2; LLPL; LPLA2; LYPLA3"
                     /EC_number="3.1.1.4"
                     /EC_number="3.1.1.32"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; LCAT-like lysophospholipase; lysophospholipase
                     3 (lysosomal phospholipase A2); 1-O-acylceramide synthase;
                     group XV phospholipase A2; lysosomal phospholipase A and
                     acyltransferase"
                     /codon_start=1
                     /product="lysosomal phospholipase A and acyltransferase
                     isoform 1 precursor"
                     /protein_id="NP_036452.1"
                     /db_xref="CCDS:CCDS10864.1"
                     /db_xref="GeneID:23659"
                     /db_xref="HGNC:HGNC:17163"
                     /db_xref="MIM:609362"
                     /translation="MGLHLRPYRVGLLPDGLLFLLLLLMLLADPALPAGRHPPVVLVP
                     GDLGNQLEAKLDKPTVVHYLCSKKTESYFTIWLNLELLLPVIIDCWIDNIRLVYNKTS
                     RATQFPDGVDVRVPGFGKTFSLEFLDPSKSSVGSYFHTMVESLVGWGYTRGEDVRGAP
                     YDWRRAPNENGPYFLALREMIEEMYQLYGGPVVLVAHSMGNMYTLYFLQRQPQAWKDK
                     YIRAFVSLGAPWGGVAKTLRVLASGDNNRIPVIGPLKIREQQRSAVSTSWLLPYNYTW
                     SPEKVFVQTPTINYTLRDYRKFFQDIGFEDGWLMRQDTEGLVEATMPPGVQLHCLYGT
                     GVPTPDSFYYESFPDRDPKICFGDGDGTVNLKSALQCQAWQSRQEHQVLLQELPGSEH
                     IEMLANATTLAYLKRVLLGP"
     exon            182..338
                     /gene="PLA2G15"
                     /gene_synonym="ACS; GXVPLA2; LLPL; LPLA2; LYPLA3"
                     /inference="alignment:Splign:2.1.0"
     exon            339..457
                     /gene="PLA2G15"
                     /gene_synonym="ACS; GXVPLA2; LLPL; LPLA2; LYPLA3"
                     /inference="alignment:Splign:2.1.0"
     exon            458..556
                     /gene="PLA2G15"
                     /gene_synonym="ACS; GXVPLA2; LLPL; LPLA2; LYPLA3"
                     /inference="alignment:Splign:2.1.0"
     exon            557..781
                     /gene="PLA2G15"
                     /gene_synonym="ACS; GXVPLA2; LLPL; LPLA2; LYPLA3"
                     /inference="alignment:Splign:2.1.0"
     exon            782..2694
                     /gene="PLA2G15"
                     /gene_synonym="ACS; GXVPLA2; LLPL; LPLA2; LYPLA3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2670..2675
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PLA2G15"
                     /gene_synonym="ACS; GXVPLA2; LLPL; LPLA2; LYPLA3"
                     /note="hexamer: AATAAA"
     polyA_site      2694
                     /gene="PLA2G15"
                     /gene_synonym="ACS; GXVPLA2; LLPL; LPLA2; LYPLA3"
                     /note="major polyA site"
ORIGIN      
        1 gagagcccag agagctgaac ctgcatcccg gacctgcggc gaccgtcgta caccatgggc
       61 ctccacctcc gcccctaccg tgtggggctg ctcccggatg gcctcctgtt cctcttgctg
      121 ctgctaatgc tgctcgcgga cccagcgctc ccggccggac gtcacccccc agtggtgctg
      181 gtccctggtg atttgggtaa ccaactggaa gccaagctgg acaagccgac agtggtgcac
      241 tacctctgct ccaagaagac cgaaagctac ttcacaatct ggctgaacct ggaactgctg
      301 ctgcctgtca tcattgactg ctggattgac aatatcaggc tggtttacaa caaaacatcc
      361 agggccaccc agtttcctga tggtgtggat gtacgtgtcc ctggctttgg gaagaccttc
      421 tcactggagt tcctggaccc cagcaaaagc agcgtgggtt cctatttcca caccatggtg
      481 gagagccttg tgggctgggg ctacacacgg ggtgaggatg tccgaggggc tccctatgac
      541 tggcgccgag ccccaaatga aaacgggccc tacttcctgg ccctccgcga gatgatcgag
      601 gagatgtacc agctgtatgg gggccccgtg gtgctggttg cccacagtat gggcaacatg
      661 tacacgctct actttctgca gcggcagccg caggcctgga aggacaagta tatccgggcc
      721 ttcgtgtcac tgggtgcgcc ctgggggggc gtggccaaga ccctgcgcgt cctggcttca
      781 ggagacaaca accggatccc agtcatcggg cccctgaaga tccgggagca gcagcggtca
      841 gctgtctcca ccagctggct gctgccctac aactacacat ggtcacctga gaaggtgttc
      901 gtgcagacac ccacaatcaa ctacacactg cgggactacc gcaagttctt ccaggacatc
      961 ggctttgaag atggctggct catgcggcag gacacagaag ggctggtgga agccacgatg
     1021 ccacctggcg tgcagctgca ctgcctctat ggtactggcg tccccacacc agactccttc
     1081 tactatgaga gcttccctga ccgtgaccct aaaatctgct ttggtgacgg cgatggtact
     1141 gtgaacttga agagtgccct gcagtgccag gcctggcaga gccgccagga gcaccaagtg
     1201 ttgctgcagg agctgccagg cagcgagcac atcgagatgc tggccaacgc caccaccctg
     1261 gcctatctga aacgtgtgct ccttgggccc tgactcctgt gccacaggac tcctgtggct
     1321 cggccgtgga cctgctgttg gcctctgggg ctgtcatggc ccacgcgttt tgcaaagttt
     1381 gtgactcacc attcaaggcc ccgagtcttg gactgtgaag catctgccat ggggaagtgc
     1441 tgtttgttat cctttctctg tggcagtgaa gaaggaagaa atgagagtct agactcaagg
     1501 gacactggat ggcaagaatg ctgctgatgg tggaactgct gtgaccttag gactggctcc
     1561 acagggtgga ctggctgggc cctggtccca gtccctgcct ggggccatgt gtccccccta
     1621 ttcctgtggg cttttcatac ttgcctactg ggccctggcc ccgcagcctt cctatgaggg
     1681 atgttactgg gctgtggtcc tgtacccaga ggtcccaggg atcggctcct ggcccctcgg
     1741 gtgacccttc ccacacacca gccacagata ggcctgccac tggtcatggg tagctagagc
     1801 tgctggcttc cctgtggctt agctggtggc cagcctgact ggcttcctgg gcgagcctag
     1861 tagctcctgc aggcaggggc agtttgttgc gttcttcgtg gttcccaggc cctgggacat
     1921 ctcactccac tcctacctcc cttaccacca ggagcattca agctctggat tgggcagcag
     1981 atgtgccccc agtcccgcag gctgtgttcc aggggccctg atttcctcgg atgtgctatt
     2041 ggccccagga ctgaagctgc ctcccttcac cctgggactg tggttccaag gatgagagca
     2101 ggggttggag ccatggcctt ctgggaacct atggagaaag ggaatccaag gaagcagcca
     2161 aggctgctcg cagcttccct gagctgcacc tcttgctaac cccaccatca cactgccacc
     2221 ctgccctagg gtctcactag taccaagtgg gtcagcacag ggctgaggat ggggctccta
     2281 tccaccctgg ccagcaccca gcttagtgct gggactagcc cagaaacttg aatgggaccc
     2341 tgagagagcc aggggtcccc tgaggccccc ctaggggctt tctgtctgcc ccagggtgct
     2401 ccatggatct ccctgtggca gcaggcatgg agagtcaggg ctgccttcat ggcagtaggc
     2461 tctaagtggg tgactggcca caggccgaga aaagggtaca gcctctaggt ggggttccca
     2521 aagacgcctt cacgctggac tgagctgctc tcccacaggg tttctgtgca gctggatttt
     2581 ctctgttgca tacatgcctg gcatctgtct ccccttgttc ctgagtggcc ccacatgggg
     2641 ctctgagcag gctgtatctg gattctggca ataaaagtac tctggatgct gtaa
//
