LOCUS       NM_001382323            3642 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens PBX/knotted 1 homeobox 2 (PKNOX2), transcript variant
            1, mRNA.
ACCESSION   NM_001382323
VERSION     NM_001382323.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Chen,L., Li,H., Liu,X., Zhang,N., Wang,K., Shi,A., Gao,H.,
            Akdis,D., Saguner,A.M., Xu,X., Osto,E., Van de Veen,W., Li,G.,
            Bayes-Genis,A., Duru,F., Song,J., Li,X. and Hu,S.
  TITLE     PBX/Knotted 1 homeobox-2 (PKNOX2) is a novel regulator of
            myocardial fibrosis
  JOURNAL   Signal Transduct Target Ther 9 (1), 94 (2024)
   PUBMED   38644381
  REMARK    GeneRIF: PBX/Knotted 1 homeobox-2 (PKNOX2) is a novel regulator of
            myocardial fibrosis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Benz,C., Ali,M., Krystkowiak,I., Simonetti,L., Sayadi,A.,
            Mihalic,F., Kliche,J., Andersson,E., Jemth,P., Davey,N.E. and
            Ivarsson,Y.
  TITLE     Proteome-scale mapping of binding sites in the unstructured regions
            of the human proteome
  JOURNAL   Mol Syst Biol 18 (1), e10584 (2022)
   PUBMED   35044719
REFERENCE   3  (bases 1 to 3642)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 3642)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 3642)
  AUTHORS   Zhang,L., Li,W., Cao,L., Xu,J., Qian,Y., Chen,H., Zhang,Y.,
            Kang,W., Gou,H., Wong,C.C. and Yu,J.
  TITLE     PKNOX2 suppresses gastric cancer through the transcriptional
            activation of IGFBP5 and p53
  JOURNAL   Oncogene 38 (23), 4590-4604 (2019)
   PUBMED   30745575
  REMARK    GeneRIF: PKNOX2 is a tumor suppressor in gastric cancer by
            activation of IGFBP5 and p53 signaling pathways
REFERENCE   6  (bases 1 to 3642)
  AUTHORS   Vaquerizas,J.M., Kummerfeld,S.K., Teichmann,S.A. and Luscombe,N.M.
  TITLE     A census of human transcription factors: function, expression and
            evolution
  JOURNAL   Nat Rev Genet 10 (4), 252-263 (2009)
   PUBMED   19274049
  REMARK    Review article
REFERENCE   7  (bases 1 to 3642)
  AUTHORS   Haller,K., Rambaldi,I., Daniels,E. and Featherstone,M.
  TITLE     Subcellular localization of multiple PREP2 isoforms is regulated by
            actin, tubulin, and nuclear export
  JOURNAL   J Biol Chem 279 (47), 49384-49394 (2004)
   PUBMED   15339927
REFERENCE   8  (bases 1 to 3642)
  AUTHORS   Haller,K., Rambaldi,I., Kovacs,E.N., Daniels,E. and Featherstone,M.
  TITLE     Prep2: cloning and expression of a new prep family member
  JOURNAL   Dev Dyn 225 (3), 358-364 (2002)
   PUBMED   12412021
REFERENCE   9  (bases 1 to 3642)
  AUTHORS   Fognani,C., Kilstrup-Nielsen,C., Berthelsen,J., Ferretti,E.,
            Zappavigna,V. and Blasi,F.
  TITLE     Characterization of PREP2, a paralog of PREP1, which defines a
            novel sub-family of the MEINOX TALE homeodomain transcription
            factors
  JOURNAL   Nucleic Acids Res 30 (9), 2043-2051 (2002)
   PUBMED   11972344
REFERENCE   10 (bases 1 to 3642)
  AUTHORS   Imoto,I., Sonoda,I., Yuki,Y. and Inazawa,J.
  TITLE     Identification and characterization of human PKNOX2, a novel
            homeobox-containing gene
  JOURNAL   Biochem Biophys Res Commun 287 (1), 270-276 (2001)
   PUBMED   11549286
  REMARK    GeneRIF: This novel PKNOX-related protein may interact with PBX
            proteins and play a role in tissue-specific regulation of
            transcription.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AP001007.5, AP003061.3, AP003069.2 and AP000708.5.
            
            On Apr 30, 2020 this sequence version replaced NM_001382323.1.
            
            Summary: Homeodomain proteins are sequence-specific transcription
            factors that share a highly conserved DNA-binding domain and play
            fundamental roles in cell proliferation, differentiation, and
            death. PKNOX2 belongs to the TALE (3-amino acid loop extension)
            class of homeodomain proteins characterized by a 3-amino acid
            extension between alpha helices 1 and 2 within the homeodomain
            (Imoto et al., 2001 [PubMed 11549286]).[supplied by OMIM, Oct
            2009].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a). Variants 1-6 all encode the same isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038195.113667.1,
                                           SRR1803615.60715.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000298282.14/ ENSP00000298282.8
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                AP001007.5         82310-82335
            27-97               AP001007.5         152604-152674
            98-204              AP003061.3         121517-121623
            205-313             AP003061.3         140982-141090
            314-453             AP003061.3         157544-157683
            454-625             AP003061.3         175249-175420
            626-814             AP003069.2         87776-87964
            815-944             AP003069.2         100098-100227
            945-1042            AP003069.2         100681-100778
            1043-1162           AP003069.2         101648-101767
            1163-1239           AP000708.5         10451-10527
            1240-1418           AP000708.5         11402-11580
            1419-3642           AP000708.5         12605-14828
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q24.2"
     gene            1..3642
                     /gene="PKNOX2"
                     /gene_synonym="PREP2"
                     /note="PBX/knotted 1 homeobox 2"
                     /db_xref="GeneID:63876"
                     /db_xref="HGNC:HGNC:16714"
                     /db_xref="MIM:613066"
     exon            1..26
                     /gene="PKNOX2"
                     /gene_synonym="PREP2"
                     /inference="alignment:Splign:2.1.0"
     exon            27..97
                     /gene="PKNOX2"
                     /gene_synonym="PREP2"
                     /inference="alignment:Splign:2.1.0"
     exon            98..204
                     /gene="PKNOX2"
                     /gene_synonym="PREP2"
                     /inference="alignment:Splign:2.1.0"
     exon            205..313
                     /gene="PKNOX2"
                     /gene_synonym="PREP2"
                     /inference="alignment:Splign:2.1.0"
     CDS             227..1645
                     /gene="PKNOX2"
                     /gene_synonym="PREP2"
                     /note="isoform a is encoded by transcript variant 1;
                     homeobox protein PKNOX2; PBX/knotted homeobox 2; homeobox
                     protein PREP-2"
                     /codon_start=1
                     /product="homeobox protein PKNOX2 isoform a"
                     /protein_id="NP_001369252.1"
                     /db_xref="CCDS:CCDS41730.1"
                     /db_xref="GeneID:63876"
                     /db_xref="HGNC:HGNC:16714"
                     /db_xref="MIM:613066"
                     /translation="MMQHASPAPALTMMATQNVPPPPYQDSPQMTATAQPPSKAQAVH
                     ISAPSAAASTPVPSAPIDPQAQLEADKRAVYRHPLFPLLTLLFEKCEQATQGSECITS
                     ASFDVDIENFVHQQEQEHKPFFSDDPELDNLMVKAIQVLRIHLLELEKVNELCKDFCN
                     RYITCLKTKMHSDNLLRNDLGGPYSPNQPSINLHSQDLLQNSPNSMSGVSNNPQGIVV
                     PASALQQGNIAMTTVNSQVVSGGALYQPVTMVTSQGQVVTQAIPQGAIQIQNTQVNLD
                     LTSLLDNEDKKSKNKRGVLPKHATNIMRSWLFQHLMHPYPTEDEKRQIAAQTNLTLLQ
                     VNNWFINARRRILQPMLDASNPDPAPKAKKIKSQHRPTQRFWPNSIAAGVLQQQGGAP
                     GTNPDGSINLDNLQSLSSDSATMAMQQAMMAAHDDSLDGTEEEDEDEMEEEEEEELEE
                     EVDELQTTNVSDLGLEHSDSLE"
     exon            314..453
                     /gene="PKNOX2"
                     /gene_synonym="PREP2"
                     /inference="alignment:Splign:2.1.0"
     exon            454..625
                     /gene="PKNOX2"
                     /gene_synonym="PREP2"
                     /inference="alignment:Splign:2.1.0"
     exon            626..814
                     /gene="PKNOX2"
                     /gene_synonym="PREP2"
                     /inference="alignment:Splign:2.1.0"
     exon            815..944
                     /gene="PKNOX2"
                     /gene_synonym="PREP2"
                     /inference="alignment:Splign:2.1.0"
     exon            945..1042
                     /gene="PKNOX2"
                     /gene_synonym="PREP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..1162
                     /gene="PKNOX2"
                     /gene_synonym="PREP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1163..1239
                     /gene="PKNOX2"
                     /gene_synonym="PREP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1240..1418
                     /gene="PKNOX2"
                     /gene_synonym="PREP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1419..3642
                     /gene="PKNOX2"
                     /gene_synonym="PREP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3619..3624
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PKNOX2"
                     /gene_synonym="PREP2"
                     /note="hexamer: AGTAAA"
     polyA_site      3642
                     /gene="PKNOX2"
                     /gene_synonym="PREP2"
                     /note="major polyA site"
ORIGIN      
        1 gtcagtctga tgaagattgg catcaggtga agtctggagc aggacttctg aggctttcta
       61 tcctccatgc tgctcactag aaaaggggct gtgaactgtg ctttggctct agcagacagg
      121 aagaaattct ggcccagctg gaagtagaaa gaggggagtg agtctcctga ggaccatctc
      181 agaggccccg ggatcacccg aacagtcctc catgtgaatc aatcccatga tgcaacatgc
      241 ctccccagcc cccgctctga cgatgatggc cacgcagaat gtcccgcccc caccctacca
      301 ggacagccca cagatgacgg caaccgccca gccaccctcc aaggcccagg ctgtccacat
      361 ctctgccccc tcagctgctg ccagcacacc tgtgcccagt gcccccatcg acccccaggc
      421 ccagctggag gctgacaagc gagctgtata caggcaccct cttttcccgc tcctgacgct
      481 gctgtttgag aaatgtgaac aggccaccca gggctctgag tgcatcacct ccgccagctt
      541 tgatgtggac atcgagaact ttgtccacca gcaggaacag gagcacaaac ccttcttcag
      601 cgatgaccca gaactggaca atctgatggt gaaggcaatc caggtcctga gaatccacct
      661 gctggagctg gagaaagtca atgaactctg caaggacttt tgtaaccgtt acatcacctg
      721 cctcaaaacc aagatgcaca gcgacaacct gctcaggaat gatctagggg ggccctactc
      781 ccccaaccag ccctccatca accttcactc acaggacctc ctgcagaatt cccccaattc
      841 catgtccgga gtctccaata acccccaggg gattgtggtc ccagcctcag cgctccagca
      901 gggcaacatc gccatgacaa ccgtcaactc acaagttgtg tcaggtggag ccttatacca
      961 accggttacc atggtaacct cccagggtca ggtggtcacc caagcaatcc cccagggagc
     1021 catccagatc cagaacacac aggttaacct tgacctcacc tccctcctgg acaatgagga
     1081 taagaagtcc aagaacaaac gaggagtctt gcccaagcat gccaccaata taatgcgttc
     1141 ttggctcttc cagcatctca tgcaccccta ccccacggag gatgagaaga ggcagatcgc
     1201 agcccagacc aacctcaccc tcctgcaagt aaacaactgg ttcatcaatg cccggaggcg
     1261 catcctgcag cccatgcttg atgccagcaa cccagatcct gcccccaaag ccaagaagat
     1321 caagtctcag caccggccca cccaaagatt ctggcccaac tccatcgctg cgggggtgct
     1381 gcagcagcag ggcggtgccc cagggacaaa ccccgatggt tccatcaact tggacaacct
     1441 gcagtccctg tcctcagaca gtgccaccat ggccatgcag caggctatga tggctgcaca
     1501 cgatgactca ttggatggga cagaagaaga ggatgaggat gagatggaag aggaggagga
     1561 ggaggagctg gaggaggagg tcgacgagct gcagacgaca aatgtcagcg acctgggctt
     1621 ggaacacagt gactccctgg agtagtcggg cagcccagat ggcactgatc actgagcagg
     1681 agaggagtgt cgccgggagg ccttcagggt gggggggaag gggacatggg caggaagcac
     1741 cgagggagtt gggccctagc ttccccaaat cagtagcttg aagaaaggca aaggagacac
     1801 ctgttccttc ccaaccaccg agcttcaatg aggaccccag ccccacttcc ctggaactgc
     1861 cgaggactct gtttggcggg gccagtcgag cagcctgtgt ggaaagacag gagtgagatc
     1921 tggactcacc aaatccctga ggatagatgg cacccatggc ccccacccac ggaaggactt
     1981 gagttgttta caagccctgc actgaggcag attggtgctg ttcgcagagt aggcctttgc
     2041 ccgggggcag acttagaagg aaggggagag acaaaggggg actgagtttc atccccagaa
     2101 gtttctcagc tcctttgaca gacattcaag ggcaggaggg agccccaaag cataaccagt
     2161 ggccagagga gtgggagggc ctgaggcatc acatcttgca gatcagaatg ggatggaatc
     2221 caccaggctc cagctcatcc ctccaaggcc ctgtctctgc gcacagcaac catggacatg
     2281 ggagaaaggg atgggagcca cagtgccctt cactctctcc tggaaaccaa ctgtaagctg
     2341 gtgggctcaa cctgtgggag gttaagagga gtcccttctg ggttgactcc aagagccaag
     2401 gagatggcag accctgggct aggaaccata tggaggtgac tttgaggcca cagctgtccc
     2461 taggtgatca cagaacttag ctcctttaac aacaggacaa tggtttttta ccctagatgt
     2521 tcccaccttc agtgctccac gccctccata gaccttcaga gaaggtgaaa ccaggttatc
     2581 tgggaatctt tccagcccgc aggtcgccac ggccatccct ttgctcccag cctggctcca
     2641 tcagcctcca gcttcctttc ttcattctgt ccttcaggga aggcagaaga aacattggaa
     2701 agcatctagt ccagtgggaa gccaggggtt ggagaaggtg ctacatccct cttcccatca
     2761 atatcctaaa tgtgggggag ggcccagaga atggcaccca agagcctgcg gggatgccca
     2821 tcccacacac cccacccagc tgttctaacc ctgctatcca cagccctgga ggaactgggg
     2881 ctcctggaag gaggaggagg ctctccactg tccaccctaa cacataccct cccacccacc
     2941 ttccagaccc ccttggttgg caccctctcc tccggttccc tctcacccca tggctgtgaa
     3001 tgacaggaca ggtcacacgt gtgttttcca ttgggtttaa tttaatggac gtgcagtttc
     3061 atttgtaaat tgtgcattgg ccacctcctt cagtggcagg atgtgagtgg ctacctggct
     3121 caactggagg ggaccccttg ggccctctgg ggcttcccct cccccacctg gttggggtag
     3181 agcaaaagga tggtcactct tccgaggtct ccctgaaatg aatgtatttc tcccccaaaa
     3241 gagctgatat ttaatgtttt aataaggatt tttgagaaac aaataacctt atttataatc
     3301 tgggtgatcc aatcattttt tactcccttt tgatgccata catagaggaa agtctagctt
     3361 ttttggcgtg agacttttgc aatgtgcagt gggataaaat gcatttcctt ttctggttcg
     3421 tttttcttgt taacacgcgc acacagacac acacacacac cgttccactc accacctgga
     3481 caggcgtccc ccagcacgga cacactggca cacaggtgcc cacatctctt cctctcagcc
     3541 cctccacctg cctaatgtta tgcaacctcc ttctgatgta tccaccaaac cagtactgaa
     3601 tgtggccgag acgttttcag taaatcttat tacctaccgt aa
//
