LOCUS       NM_004571               5300 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens PBX/knotted 1 homeobox 1 (PKNOX1), transcript variant
            1, mRNA.
ACCESSION   NM_004571
VERSION     NM_004571.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Liu,B., Zhao,S. and Liu,L.
  TITLE     PKNOX1 acts as a transcription factor of DHH and promotes the
            progression of stomach adenocarcinoma by regulating the Hedgehog
            signalling pathway
  JOURNAL   Int J Immunopathol Pharmacol 37, 3946320231208833 (2023)
   PUBMED   37864517
  REMARK    GeneRIF: PKNOX1 acts as a transcription factor of DHH and promotes
            the progression of stomach adenocarcinoma by regulating the
            Hedgehog signalling pathway.
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   Liu,Q., Tang,J., Chen,Z., Wei,L., Chen,J. and Xie,Z.
  TITLE     Polyunsaturated fatty acids ameliorate renal stone-induced renal
            tubular damage via miR-93-5p/Pknox1 axis
  JOURNAL   Nutrition 105, 111863 (2023)
   PUBMED   36356379
  REMARK    GeneRIF: Polyunsaturated fatty acids ameliorate renal stone-induced
            renal tubular damage via miR-93-5p/Pknox1 axis.
REFERENCE   3  (bases 1 to 5300)
  AUTHORS   Viard,J., Loe-Mie,Y., Daudin,R., Khelfaoui,M., Plancon,C.,
            Boland,A., Tejedor,F., Huganir,R.L., Kim,E., Kinoshita,M., Liu,G.,
            Haucke,V., Moncion,T., Yu,E., Hindie,V., Blehaut,H., Mircher,C.,
            Herault,Y., Deleuze,J.F., Rain,J.C., Simonneau,M. and
            Lepagnol-Bestel,A.M.
  TITLE     Chr21 protein-protein interactions: enrichment in proteins involved
            in intellectual disability, autism, and late-onset Alzheimer's
            disease
  JOURNAL   Life Sci Alliance 5 (12), e202101205 (2022)
   PUBMED   35914814
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5300)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 5300)
  AUTHORS   Bruckmann,C., Tamburri,S., De Lorenzi,V., Doti,N., Monti,A.,
            Mathiasen,L., Cattaneo,A., Ruvo,M., Bachi,A. and Blasi,F.
  TITLE     Mapping the native interaction surfaces of PREP1 with PBX1 by
            cross-linking mass-spectrometry and mutagenesis
  JOURNAL   Sci Rep 10 (1), 16809 (2020)
   PUBMED   33033354
  REMARK    GeneRIF: Mapping the native interaction surfaces of PREP1 with PBX1
            by cross-linking mass-spectrometry and mutagenesis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5300)
  AUTHORS   Berthelsen,J., Viggiano,L., Schulz,H., Ferretti,E., Consalez,G.G.,
            Rocchi,M. and Blasi,F.
  TITLE     PKNOX1, a gene encoding PREP1, a new regulator of Pbx activity,
            maps on human chromosome 21q22.3 and murine chromosome 17B/C
  JOURNAL   Genomics 47 (2), 323-324 (1998)
   PUBMED   9479508
REFERENCE   7  (bases 1 to 5300)
  AUTHORS   LeBrun,D.P., Matthews,B.P., Feldman,B.J. and Cleary,M.L.
  TITLE     The chimeric oncoproteins E2A-PBX1 and E2A-HLF are concentrated
            within spherical nuclear domains
  JOURNAL   Oncogene 15 (17), 2059-2067 (1997)
   PUBMED   9366523
REFERENCE   8  (bases 1 to 5300)
  AUTHORS   Chen,H., Rossier,C., Nakamura,Y., Lynn,A., Chakravarti,A. and
            Antonarakis,S.E.
  TITLE     Cloning of a novel homeobox-containing gene, PKNOX1, and mapping to
            human chromosome 21q22.3
  JOURNAL   Genomics 41 (2), 193-200 (1997)
   PUBMED   9143494
REFERENCE   9  (bases 1 to 5300)
  AUTHORS   Phelan,M.L. and Featherstone,M.S.
  TITLE     Distinct HOX N-terminal arm residues are responsible for
            specificity of DNA recognition by HOX monomers and HOX.PBX
            heterodimers
  JOURNAL   J Biol Chem 272 (13), 8635-8643 (1997)
   PUBMED   9079695
REFERENCE   10 (bases 1 to 5300)
  AUTHORS   Chang,C.P., Shen,W.F., Rozenfeld,S., Lawrence,H.J., Largman,C. and
            Cleary,M.L.
  TITLE     Pbx proteins display hexapeptide-dependent cooperative DNA binding
            with a subset of Hox proteins
  JOURNAL   Genes Dev 9 (6), 663-674 (1995)
   PUBMED   7729685
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BP369805.1, BC000735.2, AY196965.1 and CN294014.1.
            
            On May 17, 2019 this sequence version replaced NM_004571.4.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.54847.1,
                                           SRR1803616.89569.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000291547.10/ ENSP00000291547.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 BP369805.1         53-54
            3-500               BC000735.2         1-498
            501-4921            AY196965.1         531-4951
            4922-5300           CN294014.1         187-565
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="21"
                     /map="21q22.3"
     gene            1..5300
                     /gene="PKNOX1"
                     /gene_synonym="pkonx1c; PREP1"
                     /note="PBX/knotted 1 homeobox 1"
                     /db_xref="GeneID:5316"
                     /db_xref="HGNC:HGNC:9022"
                     /db_xref="MIM:602100"
     exon            1..103
                     /gene="PKNOX1"
                     /gene_synonym="pkonx1c; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            104..210
                     /gene="PKNOX1"
                     /gene_synonym="pkonx1c; PREP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             160..1470
                     /gene="PKNOX1"
                     /gene_synonym="pkonx1c; PREP1"
                     /note="isoform 1 is encoded by transcript variant 1; Pbx
                     regulating protein-1; human homeobox-containing protein;
                     homeobox protein PKNOX1; PBX/knotted homeobox 1; homeobox
                     protein PREP-1"
                     /codon_start=1
                     /product="homeobox protein PKNOX1 isoform 1"
                     /protein_id="NP_004562.2"
                     /db_xref="CCDS:CCDS13692.1"
                     /db_xref="GeneID:5316"
                     /db_xref="HGNC:HGNC:9022"
                     /db_xref="MIM:602100"
                     /translation="MMATQTLSIDSYQDGQQMQVVTELKTEQDPNCSEPDAEGVSPPP
                     VESQTPMDVDKQAIYRHPLFPLLALLFEKCEQSTQGSEGTTSASFDVDIENFVRKQEK
                     EGKPFFCEDPETDNLMVKAIQVLRIHLLELEKVNELCKDFCSRYIACLKTKMNSETLL
                     SGEPGSPYSPVQSQQIQSAITGTISPQGIVVPASALQQGNVAMATVAGGTVYQPVTVV
                     TPQGQVVTQTLSPGTIRIQNSQLQLQLNQDLSILHQDDGSSKNKRGVLPKHATNVMRS
                     WLFQHIGHPYPTEDEKKQIAAQTNLTLLQVNNWFINARRRILQPMLDSSCSETPKTKK
                     KTAQNRPVQRFWPDSIASGVAQPPPSELTMSEGAVVTITTPVNMNVDSLQSLSSDGAT
                     LAVQQVMMAGQSEDESVDSTEEDAGALAPAHISGLVLENSDSLQ"
     exon            211..338
                     /gene="PKNOX1"
                     /gene_synonym="pkonx1c; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            339..510
                     /gene="PKNOX1"
                     /gene_synonym="pkonx1c; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            511..681
                     /gene="PKNOX1"
                     /gene_synonym="pkonx1c; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            682..781
                     /gene="PKNOX1"
                     /gene_synonym="pkonx1c; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            782..879
                     /gene="PKNOX1"
                     /gene_synonym="pkonx1c; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            880..1008
                     /gene="PKNOX1"
                     /gene_synonym="pkonx1c; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..1085
                     /gene="PKNOX1"
                     /gene_synonym="pkonx1c; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1086..1258
                     /gene="PKNOX1"
                     /gene_synonym="pkonx1c; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1259..5300
                     /gene="PKNOX1"
                     /gene_synonym="pkonx1c; PREP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2273..2278
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PKNOX1"
                     /gene_synonym="pkonx1c; PREP1"
                     /note="hexamer: AAGAAA"
     polyA_site      2294
                     /gene="PKNOX1"
                     /gene_synonym="pkonx1c; PREP1"
                     /note="major polyA site"
     regulatory      4929..4934
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PKNOX1"
                     /gene_synonym="pkonx1c; PREP1"
                     /note="hexamer: ATTAAA"
     polyA_site      4947
                     /gene="PKNOX1"
                     /gene_synonym="pkonx1c; PREP1"
                     /note="major polyA site"
     regulatory      4966..4971
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PKNOX1"
                     /gene_synonym="pkonx1c; PREP1"
                     /note="hexamer: TATAAA"
     polyA_site      4987
                     /gene="PKNOX1"
                     /gene_synonym="pkonx1c; PREP1"
ORIGIN      
        1 gtgtcgcgtg gcccagcgtc ggcgtgacgg ttggacgcgg gcgcggcact gcgggtcccg
       61 attgctgcag ccgcttgtca gtgtgatgaa gattggcacc cagacaccat tcgcttttca
      121 cccaagatga tttgatgtct tataaaactc tgatgaacca tgatggctac acagacatta
      181 agtatagaca gctatcaaga tgggcaacag atgcaagtag taacagagtt aaagacagaa
      241 caagatccaa actgctctga acccgatgca gaaggagtga gccctccccc tgtggagtct
      301 cagaccccga tggatgtgga caagcaggcc atttataggc atccactatt tccattatta
      361 gctttgttgt ttgaaaaatg tgaacaatct acacagggct ctgaaggcac aacttctgcc
      421 agttttgatg tagacatcga aaattttgta agaaagcaag agaaggaagg gaaacctttc
      481 ttttgtgaag atccagaaac tgataattta atggtaaaag caatccaggt tttgcgcatt
      541 catcttcttg agctggaaaa ggttaacgaa ctctgcaaag atttctgcag tcgatacatt
      601 gcttgtctga aaacaaaaat gaacagtgaa actctgttga gtggagagcc tggaagcccg
      661 tactcaccag tgcagtccca gcagattcaa agtgccatca caggcaccat cagccctcag
      721 ggaattgtgg tgccggcgtc cgcgctgcag cagggaaacg tagccatggc gacggtggca
      781 ggtggcacag tgtatcagcc tgtcacggtc gtcactcccc aaggccaagt ggtcacacag
      841 acattgtcgc ctgggacaat taggatccag aactcccagc ttcagttaca gttaaaccaa
      901 gatctcagca tcttgcatca agatgatggt tcatctaaga acaagagggg cgtcctgcca
      961 aagcatgcca cgaacgtgat gcggtcctgg ctcttccagc acatcgggca tccctaccca
     1021 acagaggatg agaaaaaaca gattgctgct cagacaaatt tgacactact ccaagtcaac
     1081 aactggttca tcaatgccag aagacgaatt cttcagccaa tgttggattc aagttgttca
     1141 gagaccccca aaacaaagaa aaaaactgct cagaaccggc cagttcagag gttttggcct
     1201 gattctattg catcaggagt cgcacagcca ccgccgagcg agctcaccat gtcggaagga
     1261 gctgttgtca ccatcaccac gcccgtgaac atgaacgtgg acagccttca gtctctgtcc
     1321 tcggacgggg ccaccctggc ggtgcagcag gtcatgatgg cagggcagag cgaggacgag
     1381 tctgtggaca gcacagagga ggatgcgggt gccctggccc ctgcccacat cagcgggctg
     1441 gtcttggaga acagtgactc cctgcagtag gggcaggagc agacgcacct gactttttgg
     1501 agtttgcaca gcaaacattt tacacagttt tatttctaat atgttttata tgtagatata
     1561 gaagagtgca cttttgtatt tcatagtaag cttaaagcgc gtctttgccg gtgcagcgac
     1621 ttctttcaag tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgcgtgtgtg cgtgtgtgtg
     1681 gatttttaaa gaaattcttt aaaggtttaa cgctagattg tgaggaatga cacaccactc
     1741 cctccccacc ttgaatccct aattagatta aggaatagcg ctgccatttt ctaaaccgtg
     1801 atgcggttgt cacttagttc tgtggttcca gcagatctca gtgggctggt tgatttgtgt
     1861 ggcccatgga tttgaaagaa gctgctgcac ccgaaactgc cagtgtgcgg tgacaacggc
     1921 acacgcctag actgagtgtg gtttcgtcgt gagtggatgg acggcaagct tagcaagcct
     1981 aagtcccctc atgttcagtg agcctgtttc atttgctata tagaaaaaga aactcctatt
     2041 tttaccttgc tggaattatt ggataaaaaa gctattttta taaattcgtt atgaattgga
     2101 tgatgactat attgaggata aaatttctag agaagaaaca atacatgctt gctattaata
     2161 tttcaatttg gaatgttctg aattgaccaa atttaatgaa cctgcccaaa gttagctacc
     2221 gttccatggt tctttgctct ccccgggtag tgatgaacat ttactactat aaaagaaaca
     2281 gctatttaat gaaattttga tatctgcaaa tttttgttga tatgtaatgc tcagattgca
     2341 ttttacactt gatctaaaca tatatcgaaa gatatctgct aaacaggact tcaggtaagt
     2401 gaggtgaaat ggtagccagt gacccgttag gagctctcac cgtacatact ccagtctaat
     2461 ttaaatctga ccacagttgc atggtcgctt taccatgtta gctgtgtatt gttttaaaag
     2521 ttttaacttc aaaatatgtt atgcacagaa tgtttattat aaactaatat aaaatgtgct
     2581 ctaccccatt ggctcagagc tagggcaaac agcagatatt cagactttat tacttaacta
     2641 gcggacatcc ctggagtccc agcagcgagc tggtctggcg agggcacctc ggcagccccc
     2701 acgggttggc tcctacgttt gcgtttgtgg ctggtctcct ggtgtcagtg ttctcttgta
     2761 cgttgttgct ttcgactttt cagagccctc ctgctcactt gaccacgtga gatttggaat
     2821 aactgtagga cttctgtttc ctggtaacaa gatgaaccga gagagtgggc tgggttctgt
     2881 tttctttggt tttgatttgt tttcattgtt tacttaggag tggtgctttt tctcagaaaa
     2941 caggccacgg tgtttcatac agaatgtctt catatcatct gaaatggtat ggctgaagtt
     3001 catttgttta cagggtcggg aatgtcttca gttcttgaga gtcaacagta atgattggtt
     3061 gtaagccaag ggacatttta agctagtgaa gagttttttc tggaattgat ttttcccaaa
     3121 agaatatatt aattgaggtt aagaagtcag tgggaaacac acagaaattt gttttaaaat
     3181 ctttcaggag ctttactgaa agacttggtt atcaagtctt ttggggagag aatgacattt
     3241 tttttttgag acagagtttt gctcttgttg cccaggctgg agggcaatgg tgcaatctca
     3301 gctcactgca acctccacct cttatgttca agcaattctc cttccttagc ctcctgagta
     3361 gctgggatta caggcatgcg ccaccacacc cggctaattt tgtattttta gtagagatgg
     3421 gtttctccat gttggtcggg ctggtctcga actcccgacc tcaggtgatc tgcctgcctc
     3481 agcctcccaa agtgctggga ttacaggtgt gagccaccgc gcccggccga gaatgacatc
     3541 ttaaagccac cattgcgttc ctcatttgtg aactgtcttc tcatatttta agtcaagtct
     3601 ataagatcat ttttaaccta agttccaact ttgttggact ccttaaaata agcacccatg
     3661 aaagccagcc agcccttcct ccttccctca ccaccctccg tctcttcggc tgcttgctct
     3721 ttagtgtaga ttagtggaag ccattcacag aatgtagact catgtatagg tcaccgtttc
     3781 tttcccccac tagaaatcac attcactaag cagtggattg aatttaagag tgctgcccct
     3841 gcccggcgca gtagggcgtg cctctagttc cagctactcg ggagtctgag gcagaggatg
     3901 gcatgtgcca ggagtttgag gctgcagtgc actatgcttg cacctgtgaa tagccactgc
     3961 actccatctt gggcaacaca gtgagacccc catctataaa aagagagcac tgcctgaacc
     4021 tcggtggcac tgggaagctc tggcctgaga gttaaacacc catgctgaat gatacagtgt
     4081 gcattctctc cagctgcaaa ctttcttcaa ctttcctaaa ttcttactaa attcagagga
     4141 ataggataaa gatcacttag agaaagggtg cttatggaca tagcctgagt ttcctttaac
     4201 ctctctgcaa tgggtgcttt taactagctt ctacatggca agctgtttca gtttgcaaaa
     4261 tcactgccca gcatgtttga ggtcagttgg caccttaaaa cacctgttct ccagcccttg
     4321 gacagtggag agaatctgta aaagtgtgac cccctctaag atttcgtttt aaatgatcag
     4381 attcggttcc agttttattc ttgttgagtt tttccctatg aaggctcctt ttgaatgtgt
     4441 cttgagaccc aaattgagca tgttgctgtt attcctgccc tgcacctgtg gagcaggagt
     4501 ggcagggctg gctgtgctgg gttccagctc gctcccaccg agggactagc ttggcctttg
     4561 cgctttgatt ccagatagta agatgagtgg aagtgtttat cgagcatgca aaagaaaccc
     4621 tcacggctaa gggctctggg agttgtgtct gtgtgacctg agagtgatcc atctcctgcc
     4681 tgtgtgaggt agcagtgggc acttttcatt gagacaaact ccagggtgtc cagagggggt
     4741 tctgcccctt gtgaaggccc attcctggca cttagagaca gaaagaactc agcaatcttt
     4801 cgttctagtt atattcggtc tttgaaactg acaatctttg aaatgtgaat actgtaacaa
     4861 tatgttttct tggattgttg tctttaaaaa ggatttttgt gaagcaattg atttatcaaa
     4921 gcaaaaaaat taaaaataga aacttgcttt atggatgttt tattttataa agctcatgtc
     4981 accaaaagct gcttctccat tttatgtaaa acattgagaa tgttttcatg ttttctggtg
     5041 acatgtaggg tctgtagtca cccctgcata ttgagaaact aggaccacac tgcatcggac
     5101 tagtcaggtc catttacatg tccaagaatc ttttcttaaa ttccttactt tgttcattca
     5161 ttggctgttc aggcagaaaa tggaaacagg tagcttttat gttttttatt atagttttcc
     5221 acaaatctaa ttattttgat agttacaaga agataatgat tcatcagtaa gctaacataa
     5281 tccctttcat agttcatatt
//
