LOCUS       NM_006256               6070 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens protein kinase N2 (PKN2), transcript variant 1, mRNA.
ACCESSION   NM_006256
VERSION     NM_006256.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   He,C., Lin,Y., Qiu,F. and Zeng,Q.
  TITLE     Increased PKN2 and M2-Polarized Macrophages Promote HCT116 Cell
            Invasion
  JOURNAL   Crit Rev Immunol 44 (4), 13-21 (2024)
   PUBMED   38505918
  REMARK    GeneRIF: Increased PKN2 and M2-Polarized Macrophages Promote HCT116
            Cell Invasion.
REFERENCE   2  (bases 1 to 6070)
  AUTHORS   Fang,C., Wang,P., Yu,D., Zhang,X., Gou,D., Liang,L., Bai,X.,
            Xie,W., Li,H., Pu,J., Yao,Y., Wang,B., Ren,X., Ke,T., Tu,X., Xu,C.
            and Wang,Q.K.
  TITLE     Genome-Wide Association Study for Idiopathic Ventricular
            Tachyarrhythmias Identifies Key Role of CCR7 and PKN2 in Calcium
            Homeostasis and Cardiac Rhythm Maintenance
  JOURNAL   Circ Genom Precis Med 15 (5), e003603 (2022)
   PUBMED   35895078
  REMARK    GeneRIF: Genome-Wide Association Study for Idiopathic Ventricular
            Tachyarrhythmias Identifies Key Role of CCR7 and PKN2 in Calcium
            Homeostasis and Cardiac Rhythm Maintenance.
REFERENCE   3  (bases 1 to 6070)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 6070)
  AUTHORS   Jin,Y.J., Chennupati,R., Li,R., Liang,G., Wang,S., Iring,A.,
            Graumann,J., Wettschureck,N. and Offermanns,S.
  TITLE     Protein kinase N2 mediates flow-induced endothelial NOS activation
            and vascular tone regulation
  JOURNAL   J Clin Invest 131 (21) (2021)
   PUBMED   34499618
  REMARK    GeneRIF: Protein kinase N2 mediates flow-induced endothelial NOS
            activation and vascular tone regulation.
REFERENCE   5  (bases 1 to 6070)
  AUTHORS   Liu,Z., Le,Y., Chen,H., Zhu,J. and Lu,D.
  TITLE     Role of PKM2-Mediated Immunometabolic Reprogramming on Development
            of Cytokine Storm
  JOURNAL   Front Immunol 12, 748573 (2021)
   PUBMED   34759927
  REMARK    GeneRIF: Role of PKM2-Mediated Immunometabolic Reprogramming on
            Development of Cytokine Storm.
            Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6070)
  AUTHORS   Yu,W., Liu,J., Morrice,N.A. and Wettenhall,R.E.
  TITLE     Isolation and characterization of a structural homologue of human
            PRK2 from rat liver. Distinguishing substrate and lipid activator
            specificities
  JOURNAL   J Biol Chem 272 (15), 10030-10034 (1997)
   PUBMED   9092545
REFERENCE   7  (bases 1 to 6070)
  AUTHORS   Vincent,S. and Settleman,J.
  TITLE     The PRK2 kinase is a potential effector target of both Rho and Rac
            GTPases and regulates actin cytoskeletal organization
  JOURNAL   Mol Cell Biol 17 (4), 2247-2256 (1997)
   PUBMED   9121475
REFERENCE   8  (bases 1 to 6070)
  AUTHORS   Quilliam,L.A., Lambert,Q.T., Mickelson-Young,L.A., Westwick,J.K.,
            Sparks,A.B., Kay,B.K., Jenkins,N.A., Gilbert,D.J., Copeland,N.G.
            and Der,C.J.
  TITLE     Isolation of a NCK-associated kinase, PRK2, an SH3-binding protein
            and potential effector of Rho protein signaling
  JOURNAL   J Biol Chem 271 (46), 28772-28776 (1996)
   PUBMED   8910519
REFERENCE   9  (bases 1 to 6070)
  AUTHORS   Palmer,R.H., Ridden,J. and Parker,P.J.
  TITLE     Cloning and expression patterns of two members of a novel
            protein-kinase-C-related kinase family
  JOURNAL   Eur J Biochem 227 (1-2), 344-351 (1995)
   PUBMED   7851406
REFERENCE   10 (bases 1 to 6070)
  AUTHORS   Palmer,R.H., Ridden,J. and Parker,P.J.
  TITLE     Identification of multiple, novel, protein kinase C-related gene
            products
  JOURNAL   FEBS Lett 356 (1), 5-8 (1994)
   PUBMED   7988719
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC119426.2, U33052.1 and AL136381.12.
            
            On Nov 22, 2018 this sequence version replaced NM_006256.3.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.38853.1,
                                           SRR1660807.26047.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000370521.8/ ENSP00000359552.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-309               AC119426.2         23632-23940         c
            310-3554            U33052.1           11-3255
            3555-6070           AL136381.12        896-3411            c
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p22.2"
     gene            1..6070
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /note="protein kinase N2"
                     /db_xref="GeneID:5586"
                     /db_xref="HGNC:HGNC:9406"
                     /db_xref="MIM:602549"
     exon            1..356
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /inference="alignment:Splign:2.1.0"
     CDS             309..3263
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /EC_number="2.7.11.13"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cardiolipin-activated protein kinase Pak2; protein kinase
                     C-like 2; serine/threonine-protein kinase N2;
                     protein-kinase C-related kinase 2; PKN gamma"
                     /codon_start=1
                     /product="serine/threonine-protein kinase N2 isoform 1"
                     /protein_id="NP_006247.1"
                     /db_xref="CCDS:CCDS714.1"
                     /db_xref="GeneID:5586"
                     /db_xref="HGNC:HGNC:9406"
                     /db_xref="MIM:602549"
                     /translation="MASNPERGEILLTELQGDSRSLPFSENVSAVQKLDFSDTMVQQK
                     LDDIKDRIKREIRKELKIKEGAENLRKVTTDKKSLAYVDNILKKSNKKLEELHHKLQE
                     LNAHIVVSDPEDITDCPRTPDTPNNDPRCSTSNNRLKALQKQLDIELKVKQGAENMIQ
                     MYSNGSSKDRKLHGTAQQLLQDSKTKIEVIRMQILQAVQTNELAFDNAKPVISPLELR
                     MEELRHHFRIEFAVAEGAKNVMKLLGSGKVTDRKALSEAQARFNESSQKLDLLKYSLE
                     QRLNEVPKNHPKSRIIIEELSLVAASPTLSPRQSMISTQNQYSTLSKPAALTGTLEVR
                     LMGCQDILENVPGRSKATSVALPGWSPSETRSSFMSRTSKSKSGSSRNLLKTDDLSND
                     VCAVLKLDNTVVGQTSWKPISNQSWDQKFTLELDRSRELEISVYWRDWRSLCAVKFLR
                     LEDFLDNQRHGMCLYLEPQGTLFAEVTFFNPVIERRPKLQRQKKIFSKQQGKTFLRAP
                     QMNINIATWGRLVRRAIPTVNHSGTFSPQAPVPTTVPVVDVRIPQLAPPASDSTVTKL
                     DFDLEPEPPPAPPRASSLGEIDESSELRVLDIPGQDSETVFDIQNDRNSILPKSQSEY
                     KPDTPQSGLEYSGIQELEDRRSQQRFQFNLQDFRCCAVLGRGHFGKVLLAEYKNTNEM
                     FAIKALKKGDIVARDEVDSLMCEKRIFETVNSVRHPFLVNLFACFQTKEHVCFVMEYA
                     AGGDLMMHIHTDVFSEPRAVFYAACVVLGLQYLHEHKIVYRDLKLDNLLLDTEGFVKI
                     ADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTETSYTRAVDWWGLGVLIYEMLVGESP
                     FPGDDEEEVFDSIVNDEVRYPRFLSTEAISIMRRLLRRNPERRLGASEKDAEDVKKHP
                     FFRLIDWSALMDKKVKPPFIPTIRGREDVSNFDDEFTSEAPILTPPREPRILSEEEQE
                     MFRDFDYIADWC"
     exon            357..657
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /inference="alignment:Splign:2.1.0"
     exon            658..812
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /inference="alignment:Splign:2.1.0"
     exon            813..930
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /inference="alignment:Splign:2.1.0"
     exon            931..1076
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1293
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /inference="alignment:Splign:2.1.0"
     exon            1294..1479
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /inference="alignment:Splign:2.1.0"
     exon            1480..1589
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /inference="alignment:Splign:2.1.0"
     exon            1590..1733
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /inference="alignment:Splign:2.1.0"
     exon            1734..1809
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /inference="alignment:Splign:2.1.0"
     exon            1810..1984
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /inference="alignment:Splign:2.1.0"
     exon            1985..2111
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /inference="alignment:Splign:2.1.0"
     exon            2112..2242
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /inference="alignment:Splign:2.1.0"
     exon            2243..2318
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /inference="alignment:Splign:2.1.0"
     exon            2319..2410
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /inference="alignment:Splign:2.1.0"
     exon            2411..2587
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /inference="alignment:Splign:2.1.0"
     exon            2588..2650
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /inference="alignment:Splign:2.1.0"
     exon            2651..2727
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /inference="alignment:Splign:2.1.0"
     exon            2728..2870
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /inference="alignment:Splign:2.1.0"
     exon            2871..2978
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /inference="alignment:Splign:2.1.0"
     exon            2979..3059
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /inference="alignment:Splign:2.1.0"
     exon            3060..6070
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5591..5596
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /note="hexamer: ATTAAA"
     polyA_site      5620
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
     regulatory      5718..5723
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /note="hexamer: AATAAA"
     polyA_site      5737
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /note="major polyA site"
     regulatory      6051..6056
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
                     /note="hexamer: AATAAA"
     polyA_site      6070
                     /gene="PKN2"
                     /gene_synonym="Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7"
ORIGIN      
        1 gcggccgcag cgcccgcacg gagaggcttg aggcggctcc tggcgtcgcc cagagggagc
       61 gactagacga acagtccggt gagggcggcg agaggaagcc cgctacgagt gccctagctc
      121 cccgccgctc tcgatgaacc ggacggaata agccgcgcct ccagcagggg ctgcgcctcc
      181 atgaatccct agttgttttt ttttttttct ttctctcccc tctcctcacc cccaccccga
      241 gccccgtccc gccttctccc ttcgccagag gcggccgcgt ccaggtgcgg agtccatacc
      301 ggagcgcaat ggcgtccaac cccgaacggg gggagattct gctcacggaa ctgcaggggg
      361 attcccgaag tcttccgttt tctgagaatg tgagtgctgt tcaaaaatta gacttttcag
      421 atacaatggt gcagcagaaa ttggatgata tcaaggatcg aattaagaga gaaataagga
      481 aagaactgaa aatcaaagaa ggagctgaaa atctgaggaa agtcacaaca gataaaaaaa
      541 gtttggctta tgtagacaac attttgaaaa aatcaaataa aaaattagaa gaactacatc
      601 acaagctgca ggaattaaat gcacatattg ttgtatcaga tccagaagat attacagatt
      661 gcccaaggac tccagatact ccaaataatg accctcgttg ttctactagc aacaatagat
      721 tgaaggcctt acaaaaacaa ttggatatag aacttaaagt aaaacaaggt gcagagaata
      781 tgatacagat gtattcaaat ggatcttcaa aggatcggaa actccatggt acagctcagc
      841 aactgctcca ggacagcaag acaaaaatag aagtcatacg aatgcagatt cttcaggcag
      901 tccagactaa tgaattggct tttgataatg caaaacctgt gataagtcct cttgaacttc
      961 ggatggaaga attaaggcat cattttagga tagagtttgc agtagcagaa ggtgcaaaga
     1021 atgtaatgaa attacttggc tcaggaaaag taacagacag aaaagcactt tcagaagctc
     1081 aagcaagatt taatgaatca agtcagaagt tggacctttt aaagtattca ttagagcaaa
     1141 gattaaacga agtccccaag aatcatccca aaagcaggat tattattgaa gaactttcac
     1201 ttgttgctgc atcaccaaca ctaagtccac gtcaaagtat gatatctacg caaaatcaat
     1261 atagtacact atccaaacca gcagcactaa caggtacttt ggaagttcgt cttatgggct
     1321 gccaagatat cctagagaat gtccctggac ggtcaaaagc aacatcagtt gcactgcctg
     1381 gttggagtcc aagtgaaacc agatcatctt tcatgagcag aacgagtaaa agtaaaagcg
     1441 gaagtagtcg aaatcttcta aaaaccgatg acttgtccaa tgatgtctgt gctgttttga
     1501 agctcgataa tactgtggtt ggccaaacta gctggaaacc catttccaat cagtcatggg
     1561 accagaagtt tacactggaa ctggacaggt cacgtgaact ggaaatttca gtttattggc
     1621 gtgattggcg gtctctgtgt gctgtaaaat ttctgaggtt agaagatttt ttagacaacc
     1681 aacggcatgg catgtgtctc tatttggaac cacagggtac tttatttgca gaggttacct
     1741 tttttaatcc agttattgaa agaagaccaa aacttcaaag acaaaagaaa attttttcaa
     1801 agcaacaagg caaaacattt ctcagagctc ctcaaatgaa tattaatatt gccacttggg
     1861 gaaggctagt aagaagagct attcctacag taaatcattc tggcaccttc agccctcaag
     1921 ctcctgtgcc tactacagtg ccagtggttg atgtacgcat ccctcaacta gcacctccag
     1981 ctagtgattc tacagtaacc aaattggact ttgatcttga gcctgaacct cctccagccc
     2041 caccacgagc ttcttctctt ggagaaatag atgaatcttc tgaattaaga gttttggata
     2101 taccaggaca ggattcagag actgtttttg atattcagaa tgacagaaat agtatacttc
     2161 caaaatctca atctgaatac aagcctgata ctcctcagtc aggcctagaa tatagtggta
     2221 ttcaagaact tgaggacaga agatctcagc aaaggtttca gtttaatcta caagatttca
     2281 ggtgttgtgc tgtcttggga agaggacatt ttggaaaggt gcttttagct gaatataaaa
     2341 acacaaatga gatgtttgct ataaaagcct taaagaaagg agatattgtg gctcgagatg
     2401 aagtagacag cctgatgtgt gaaaaaagaa tttttgaaac tgtgaatagt gtaaggcatc
     2461 cctttttggt gaaccttttt gcatgtttcc aaaccaaaga gcatgtttgc tttgtaatgg
     2521 aatatgctgc cggtggggac ctaatgatgc acattcatac tgatgtcttt tctgaaccaa
     2581 gagctgtatt ttatgctgct tgtgtagttc ttgggttgca gtatttacat gaacacaaaa
     2641 ttgtttatag agatttgaaa ttggataact tattgctaga tacagagggc tttgtgaaaa
     2701 ttgctgattt tggtctttgc aaagaaggaa tgggatatgg agatagaaca agcacatttt
     2761 gtggcactcc tgaatttctt gccccagaag tattaacaga aacttcttat acaagggctg
     2821 tagattggtg gggccttggc gtgcttatat atgaaatgct tgttggtgag tctccctttc
     2881 ctggtgatga tgaagaggaa gtttttgaca gtattgtaaa tgatgaagta aggtatccaa
     2941 ggttcttatc tacagaagcc atttctataa tgagaaggct gttaagaaga aatcctgaac
     3001 ggcgccttgg ggctagcgag aaagatgcag aggatgtaaa aaagcaccca tttttccggc
     3061 taattgattg gagcgctctg atggacaaaa aagtaaagcc accatttata cctaccataa
     3121 gaggacgaga agatgttagt aattttgatg atgaatttac ctcagaagca cctattctga
     3181 ctccacctcg agaaccaagg atactttcgg aagaggagca ggaaatgttc agagattttg
     3241 actacattgc tgattggtgt taagttgcta gacactgcga aaccaagctg actcacaaga
     3301 agacctctta aaaatagcaa cccttcattt gctctctgtg ccaccaatag cttctgagtt
     3361 ttttgttgtt gttgttttta ttgaaacacg tgaagatttg tttaaaagta ccattctaat
     3421 acttcttcaa aagtggctcc tcattgtact tcagcgtaaa tatgagcact ggaaacagtt
     3481 tcatggagtt taagttgagt gaacatcggc catgaaaatc catcacgaat acttttggat
     3541 caatagtcta tttttaaaaa gaaagaaaaa aaccactttt ttatagtccc tagctttgcc
     3601 atatgcccgc cttaagtgga aggaaagtta atcacttaac tatgttttat aaaaagaaaa
     3661 aagggcttgg aatgctatta ctgttcacac aaagtatgat tctgtttgaa taaggcaaat
     3721 gctccttttt ttaaaaaaaa agacattact gtaatatcaa aaaccgtggc agtttgtata
     3781 caactcgggg cttgattttt tttaaaaaaa cagaatgaat tgatgtctta ttttataaat
     3841 gttctatatt tattaggaga aaactttata ttgccttttt tatcaatcat gtaacaggct
     3901 tatagctttc caacagagct gcttgccaaa caattttttt tgtttattaa acagtgctga
     3961 aacaaacagg atcagcattt acttaagatg ttaagaatga ggacttttaa tcagccgaac
     4021 caagatattg ttacctgtat gcattcccaa agtctagatg ctcagtatgt tcagtcatat
     4081 ctttcagaat cagtgaaccg attacccttt ttttggtatt cactctacat ctgccaacct
     4141 agttcacctt ggttttgtgt ctgctgtaga agggaaccat aacttggtta aaccgtaggg
     4201 attatcattg tatacatgct gtgaacatgt atatgtgcaa gttttaatgt attctatgtt
     4261 gtaggcttat tatttaattt taccacttca tcacttttgt acagtggcca agcagacacc
     4321 tcaaactcca gcatttacat taagtgcatt tgtacatttt aggccactgg atccagattt
     4381 tatattggca gttattgagg gcaaaagcaa tatattgtaa cagaatgtat aaatattttt
     4441 gataaaacag tctatatttt attaaaaaat gaattataac actaaagctg tacctcaaaa
     4501 gtctcaaaaa taatttgatg aaatatttta tacaactctt caaaggatcc gtctgtggct
     4561 ttttatactc ttttagggtt gtctttttac aaaccatgac tttccacttg cctgtagttt
     4621 tttgtttgtt ttggtttggt ttgattttat atttttttct cctaatctat gactttattg
     4681 ttttttctta gtttagtaat agcatctttg atcctgtgct tagcatgtta gggtcattat
     4741 acctcaggaa tagcaagctg ttaagtaacc atactgaatt aactatttaa ttacagtgag
     4801 ctcatctctt aaaaattgtt caggtgtaaa tcttatgaga aacatgaaaa agcacactga
     4861 tttatggaga gttgagctaa aaacatttat aaatatttgc tgggatgttt tagcatcttt
     4921 tcattgtact ctgagaacaa ttaaaattgt ccagagatca tttatattac cttccaaatt
     4981 gtttattacc caagatcctt tgggagaaaa tcttatagaa tgggggaaca atatgtggtt
     5041 taaagttatt ttaaaatgcc agttaatttc ctcggcaaag gacaatagag gaacttagct
     5101 taattgtctg gctttaattt aaactgtgtt aatacaacat taaaataaaa cactaaatac
     5161 ttttgaggta cagtctgctc acttttttgg ttctcaaata gcaaagaaag taatgtctaa
     5221 aataggctct tagcattaaa aactgacagc atttcgtaac tacacagtgt acagaatttt
     5281 tttttaattg aagtcaatca ctaaaaaaat tagaaggcag ggtctattta cacaattaag
     5341 ctgaagaaag cactaatttt ttgtagttta aaatatatat atattttagt cagatttaaa
     5401 attttaagct ttatagagtg taaatatatt ttgctattac tgtatgtgta tgtgactgct
     5461 caagcacttt gtaaagaaat taggatattt tagaatggta cactatgcat tcaaatgaaa
     5521 tgtgcctttc actatgtcat tctaagaaaa caagtgtttt ttgcagtcat aaattatcac
     5581 aaatgcacaa attaaagtct atatagattg aaatttgtaa ctttggtttt aagtattctt
     5641 tccttttaga aacttcctaa tgattaaaat atactttaac ttttctgtgt tcaaaatctc
     5701 aaagactaat taactttaat aaacattctt gcaagtactt tttgtcctag tgaaggctta
     5761 aaaccatgaa cattcagagc aaactgtcca cttcattggg aataatggga aaagtttgta
     5821 ttctgctcct gtaaggtcag tagcatcttt tatttgaagc atatttatgg actgcttact
     5881 gtgtatagat actgagaata ggaagttttt ttttttaaag caacaaaatg ttgatgaatg
     5941 aagaacagcc tgttttaatt aagatgcaag caccagtatt gtatgtactt ttctcttgtt
     6001 tgaggttagc ttattttaat ttaggacctg gcttctcaaa aaggcaaatg aataaaacag
     6061 aatactagta
//
