LOCUS       NM_002741               3120 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens protein kinase N1 (PKN1), transcript variant 2, mRNA.
ACCESSION   NM_002741
VERSION     NM_002741.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Govorov,I., Attarha,S., Kovalevska,L., Andersson,E., Kashuba,E. and
            Mints,M.
  TITLE     Upregulation of PKN1 as a Prognosis Biomarker for Endometrial
            Cancer
  JOURNAL   Cancer Control 29, 10732748221094797 (2022)
   PUBMED   35533253
  REMARK    GeneRIF: Upregulation of PKN1 as a Prognosis Biomarker for
            Endometrial Cancer.
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 3120)
  AUTHORS   Zeng,R., Wang,Z., Li,X., Chen,Y., Yang,S. and Dong,J.
  TITLE     Cyclin-dependent kinase 1-mediated phosphorylation of protein
            kinase N1 promotes anchorage-independent growth and migration
  JOURNAL   Cell Signal 69, 109546 (2020)
   PUBMED   31981797
  REMARK    GeneRIF: Cyclin-dependent kinase 1-mediated phosphorylation of
            protein kinase N1 promotes anchorage-independent growth and
            migration.
REFERENCE   4  (bases 1 to 3120)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 3120)
  AUTHORS   Patel,H., Li,J., Herrero,A., Kroboth,J., Byron,A., Kriegsheim,A.V.,
            Brunton,V., Carragher,N., Hurd,T. and Frame,M.
  TITLE     Novel roles of PRK1 and PRK2 in cilia and cancer biology
  JOURNAL   Sci Rep 10 (1), 3902 (2020)
   PUBMED   32127582
  REMARK    GeneRIF: Novel roles of PRK1 and PRK2 in cilia and cancer biology.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3120)
  AUTHORS   Palmer,R.H., Schonwasser,D.C., Rahman,D., Pappin,D.J., Herget,T.
            and Parker,P.J.
  TITLE     PRK1 phosphorylates MARCKS at the PKC sites: serine 152, serine 156
            and serine 163
  JOURNAL   FEBS Lett 378 (3), 281-285 (1996)
   PUBMED   8557118
REFERENCE   7  (bases 1 to 3120)
  AUTHORS   Palmer,R.H., Ridden,J. and Parker,P.J.
  TITLE     Cloning and expression patterns of two members of a novel
            protein-kinase-C-related kinase family
  JOURNAL   Eur J Biochem 227 (1-2), 344-351 (1995)
   PUBMED   7851406
REFERENCE   8  (bases 1 to 3120)
  AUTHORS   Palmer,R.H., Ridden,J. and Parker,P.J.
  TITLE     Identification of multiple, novel, protein kinase C-related gene
            products
  JOURNAL   FEBS Lett 356 (1), 5-8 (1994)
   PUBMED   7988719
REFERENCE   9  (bases 1 to 3120)
  AUTHORS   Chu,W., Presky,D.H., Danho,W., Swerlick,R.A. and Burns,D.K.
  TITLE     Identification and characterization of DBK, a novel putative
            serine/threonine protein kinase from human endothelial cells
  JOURNAL   Eur J Biochem 225 (2), 695-702 (1994)
   PUBMED   7957185
REFERENCE   10 (bases 1 to 3120)
  AUTHORS   Mukai,H. and Ono,Y.
  TITLE     A novel protein kinase with leucine zipper-like sequences: its
            catalytic domain is highly homologous to that of protein kinase C
  JOURNAL   Biochem Biophys Res Commun 199 (2), 897-904 (1994)
   PUBMED   8135837
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC008569.7.
            
            On Jun 2, 2019 this sequence version replaced NM_002741.4.
            
            Summary: The protein encoded by this gene belongs to the protein
            kinase C superfamily. This kinase is activated by Rho family of
            small G proteins and may mediate the Rho-dependent signaling
            pathway. This kinase can be activated by phospholipids and by
            limited proteolysis. The 3-phosphoinositide dependent protein
            kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase,
            which may mediate insulin signals to the actin cytoskeleton. The
            proteolytic activation of this kinase by caspase-3 or related
            proteases during apoptosis suggests its role in signal transduction
            related to apoptosis. Alternatively spliced transcript variants
            encoding distinct isoforms have been observed. [provided by RefSeq,
            Jul 2008].
            
            Transcript Variant: This variant (2) uses an alternate exon at the
            5' end compared to variant 1, which includes a part of the coding
            region. The resulting isoform (2) has a distinct and shorter
            N-terminus, as compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC040061.1, S75546.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000242783.11/ ENSP00000242783.7
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-237               AC008569.7         167915-168151
            238-538             AC008569.7         175752-176052
            539-690             AC008569.7         178079-178230
            691-826             AC008569.7         181035-181170
            827-972             AC008569.7         184927-185072
            973-1186            AC008569.7         185505-185718
            1187-1381           AC008569.7         186438-186632
            1382-1491           AC008569.7         192640-192749
            1492-1635           AC008569.7         192841-192984
            1636-1711           AC008569.7         198276-198351
            1712-1853           AC008569.7         198437-198578
            1854-1947           AC008569.7         198669-198762
            1948-2024           AC008569.7         202174-202250
            2025-2100           AC008569.7         202327-202402
            2101-2192           AC008569.7         202485-202576
            2193-2369           AC008569.7         203950-204126
            2370-2432           AC008569.7         204361-204423
            2433-2509           AC008569.7         204521-204597
            2510-2652           AC008569.7         204772-204914
            2653-2760           AC008569.7         205184-205291
            2761-2841           AC008569.7         205380-205460
            2842-3120           AC008569.7         206190-206468
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.12"
     gene            1..3120
                     /gene="PKN1"
                     /gene_synonym="DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1;
                     PRKCL1"
                     /note="protein kinase N1"
                     /db_xref="GeneID:5585"
                     /db_xref="HGNC:HGNC:9405"
                     /db_xref="MIM:601032"
     exon            1..237
                     /gene="PKN1"
                     /gene_synonym="DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1;
                     PRKCL1"
                     /inference="alignment:Splign:2.1.0"
     CDS             217..3045
                     /gene="PKN1"
                     /gene_synonym="DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1;
                     PRKCL1"
                     /EC_number="2.7.11.13"
                     /note="isoform 2 is encoded by transcript variant 2;
                     protein kinase C-like 1; protein kinase C-related kinase
                     1; serine/threonine protein kinase N; protein kinase
                     C-like PKN; serine/threonine-protein kinase N1;
                     serine-threonine kinase N; protein kinase PKN-alpha;
                     protease-activated kinase 1"
                     /codon_start=1
                     /product="serine/threonine-protein kinase N1 isoform 2"
                     /protein_id="NP_002732.3"
                     /db_xref="CCDS:CCDS42513.1"
                     /db_xref="GeneID:5585"
                     /db_xref="HGNC:HGNC:9405"
                     /db_xref="MIM:601032"
                     /translation="MASDAVQSEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLRR
                     EIRKELKLKEGAENLRRATTDLGRSLGPVELLLRGSSRRLDLLHQQLQELHAHVVLPD
                     PAATHDGPQSPGAGGPTCSATNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSTKDR
                     KLLLTAQQMLQDSKTKIDIIRMQLRRALQAGQLENQAAPDDTQGSPDLGAVELRIEEL
                     RHHFRVEHAVAEGAKNVLRLLSAAKAPDRKAVSEAQEKLTESNQKLGLLREALERRLG
                     ELPADHPKGRLLREELAAASSAAFSTRLAGPFPATHYSTLCKPAPLTGTLEVRVVGCR
                     DLPETIPWNPTPSMGGPGTPDSRPPFLSRPARGLYSRSGSLSGRSSLKAEAENTSEVS
                     TVLKLDNTVVGQTSWKPCGPNAWDQSFTLELERARELELAVFWRDQRGLCALKFLKLE
                     DFLDNERHEVQLDMEPQGCLVAEVTFRNPVIERIPRLRRQKKIFSKQQGKAFQRARQM
                     NIDVATWVRLLRRLIPNATGTGTFSPGASPGSEARTTGDISVEKLNLGTDSDSSPQKS
                     SRDPPSSPSSLSSPIQESTAPELPSETQETPGPALCSPLRKSPLTLEDFKFLAVLGRG
                     HFGKVLLSEFRPSGELFAIKALKKGDIVARDEVESLMCEKRILAAVTSAGHPFLVNLF
                     GCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAIFYSACVVLGLQFLHEHKIVYRD
                     LKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTDTSYTRAVDW
                     WGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPER
                     RLGSSERDAEDVKKQPFFRTLGWEALLARRLPPPFVPTLSGRTDVSNFDEEFTGEAPT
                     LSPPRDARPLTAAEQAAFLDFDFVAGGC"
     exon            238..538
                     /gene="PKN1"
                     /gene_synonym="DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1;
                     PRKCL1"
                     /inference="alignment:Splign:2.1.0"
     exon            539..690
                     /gene="PKN1"
                     /gene_synonym="DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1;
                     PRKCL1"
                     /inference="alignment:Splign:2.1.0"
     exon            691..826
                     /gene="PKN1"
                     /gene_synonym="DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1;
                     PRKCL1"
                     /inference="alignment:Splign:2.1.0"
     exon            827..972
                     /gene="PKN1"
                     /gene_synonym="DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1;
                     PRKCL1"
                     /inference="alignment:Splign:2.1.0"
     exon            973..1186
                     /gene="PKN1"
                     /gene_synonym="DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1;
                     PRKCL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1187..1381
                     /gene="PKN1"
                     /gene_synonym="DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1;
                     PRKCL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..1491
                     /gene="PKN1"
                     /gene_synonym="DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1;
                     PRKCL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1492..1635
                     /gene="PKN1"
                     /gene_synonym="DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1;
                     PRKCL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1636..1711
                     /gene="PKN1"
                     /gene_synonym="DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1;
                     PRKCL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1712..1853
                     /gene="PKN1"
                     /gene_synonym="DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1;
                     PRKCL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1854..1947
                     /gene="PKN1"
                     /gene_synonym="DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1;
                     PRKCL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1948..2024
                     /gene="PKN1"
                     /gene_synonym="DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1;
                     PRKCL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2025..2100
                     /gene="PKN1"
                     /gene_synonym="DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1;
                     PRKCL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2101..2192
                     /gene="PKN1"
                     /gene_synonym="DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1;
                     PRKCL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2193..2369
                     /gene="PKN1"
                     /gene_synonym="DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1;
                     PRKCL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2370..2432
                     /gene="PKN1"
                     /gene_synonym="DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1;
                     PRKCL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2433..2509
                     /gene="PKN1"
                     /gene_synonym="DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1;
                     PRKCL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2510..2652
                     /gene="PKN1"
                     /gene_synonym="DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1;
                     PRKCL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2653..2760
                     /gene="PKN1"
                     /gene_synonym="DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1;
                     PRKCL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2761..2841
                     /gene="PKN1"
                     /gene_synonym="DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1;
                     PRKCL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2842..3120
                     /gene="PKN1"
                     /gene_synonym="DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1;
                     PRKCL1"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      3120
                     /gene="PKN1"
                     /gene_synonym="DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1;
                     PRKCL1"
                     /note="major polyA site"
ORIGIN      
        1 acgtgaccag gcccggcggc cgctggggcc ggagggcgcg gcgcggggac gcggcggcct
       61 ggccaggcga ggcgggagcc gagcaggccg gagcgggccg cgcgccgggg agggtcgcgc
      121 cgcgcccgcc ccctccccgc cacgcccggg gcgcacgcgc gctcctctgg ccgcccctcc
      181 ctccgcgcgg ggacccctgg cgggcggcag gaggacatgg ccagcgacgc cgtgcagagt
      241 gagcctcgca gctggtccct gctagagcag ctgggcctgg ccggggcaga cctggcggcc
      301 cccggggtac agcagcagct ggagctggag cgggagcggc tgcggcggga aatccgcaag
      361 gagctgaagc tgaaggaggg tgctgagaac ctgcggcggg ccaccactga cctgggccgc
      421 agcctgggcc ccgtagagct gctgctgcgg ggctcctcgc gccgcctcga cctgctgcac
      481 cagcagctgc aggagctgca cgcccacgtg gtgcttcccg acccggcggc cacccacgat
      541 ggcccccagt cccctggtgc gggtggcccc acctgctcgg ccaccaacct gagccgcgtg
      601 gcgggcctgg agaagcagtt ggccattgag ctgaaggtga agcagggggc ggagaacatg
      661 atccagacct acagcaatgg cagcaccaag gaccggaagc tgctgctgac agcccagcag
      721 atgttgcagg acagtaagac caagattgac atcatccgca tgcaactccg ccgggcgctg
      781 caggccggcc agctggagaa ccaggcagcc ccggatgaca cccaagggag tcctgacctg
      841 ggggctgtgg agctgcgcat cgaagagctg cggcaccact tccgagtgga gcacgcggtg
      901 gccgagggtg ccaagaacgt actgcgcctg ctcagcgctg ccaaggcccc ggaccgcaag
      961 gcagtcagcg aggcccagga gaaattaaca gaatccaacc agaagctggg gctgctgcgg
     1021 gaggctctgg agcggagact tggggagctg cccgccgacc accccaaggg gcggctgctg
     1081 cgagaagagc tcgctgcggc ctcctccgct gccttcagca cccgcctggc cgggcccttt
     1141 cccgccacgc actacagcac cctgtgcaag cccgcgccgc tcacagggac cctggaggta
     1201 cgagtggtgg gctgcagaga cctcccagag accatcccgt ggaaccctac cccctcaatg
     1261 gggggacctg ggaccccaga cagccgcccc cccttcctga gccgcccagc ccggggcctt
     1321 tacagccgaa gcggaagcct cagtggccgg agcagcctca aagcagaagc cgagaacacc
     1381 agtgaagtca gcactgtgct taagctggat aacacagtgg tggggcagac gtcttggaag
     1441 ccatgtggcc ccaatgcctg ggaccagagc ttcactctgg agctggaaag ggcacgggaa
     1501 ctggagttgg ctgtgttctg gcgggaccag cggggcctgt gtgccctcaa attcctgaag
     1561 ttggaggatt tcttggacaa tgagaggcat gaggtgcagc tggacatgga accccagggc
     1621 tgcctggtgg ctgaggtcac cttccgcaac cctgtcattg agaggattcc tcggctccga
     1681 cggcagaaga aaattttctc caagcagcaa gggaaggcgt tccagcgtgc taggcagatg
     1741 aacatcgatg tcgccacgtg ggtgcggctg ctccggaggc tcatccccaa tgccacgggc
     1801 acaggcacct ttagccctgg ggcttctcca ggatccgagg cccggaccac gggtgacata
     1861 tcggtggaga agctgaacct cggcactgac tcggacagct cacctcagaa gagctcgcgg
     1921 gatcctcctt ccagcccatc gagcctgagc tcccccatcc aggaatccac tgctcccgag
     1981 ctgccttcgg agacccagga gaccccaggc cccgccctgt gcagccctct gaggaagtca
     2041 cctctgaccc tcgaagattt caagttcctg gcggtgctgg gccggggtca ttttgggaag
     2101 gtgctcctct ccgaattccg gcccagtggg gagctgttcg ccatcaaggc tctgaagaaa
     2161 ggggacattg tggcccgaga cgaggtggag agcctgatgt gtgagaagcg gatattggcg
     2221 gcagtgacca gtgcgggaca ccccttcctg gtgaacctct tcggctgttt ccagacaccg
     2281 gagcacgtgt gcttcgtgat ggagtactcg gccggtgggg acctgatgct gcacatccac
     2341 agcgacgtgt tctctgagcc ccgtgccatc ttttattccg cctgcgtggt gctgggccta
     2401 cagtttcttc acgaacacaa gatcgtctac agggacctga agttggacaa tttgctcctg
     2461 gacaccgagg gctacgtcaa gatcgcagac tttggcctct gcaaggaggg gatgggctat
     2521 ggggaccgga ccagcacatt ctgtgggacc ccggagttcc tggcccctga ggtgctgacg
     2581 gacacgtcgt acacgcgagc tgtggactgg tggggactgg gtgtgctgct ctacgagatg
     2641 ctggttggcg agtccccatt cccaggggat gatgaggagg aggtcttcga cagcatcgtc
     2701 aacgacgagg ttcgctaccc ccgcttcctg tcggccgaag ccatcggcat catgagaagg
     2761 ctgcttcgga ggaacccaga gcggaggctg ggatctagcg agagagatgc agaagatgtg
     2821 aagaaacagc ccttcttcag gactctgggc tgggaagccc tgttggcccg gcgcctgcca
     2881 ccgccctttg tgcccacgct gtccggccgc accgacgtca gcaacttcga cgaggagttc
     2941 accggggagg cccccacact gagcccgccc cgcgacgcgc ggcccctcac agccgcggag
     3001 caggcagcct tcctggactt cgacttcgtg gccgggggct gctagccccc tcccctgccc
     3061 ctgcccctgc ccctgcccga gagctcttag tttttaaaaa ggcctttggg atttgccgga
//
