LOCUS       NM_004203               2061 bp    mRNA    linear   PRI 27-JUL-2024
DEFINITION  Homo sapiens protein kinase, membrane associated tyrosine/threonine
            1 (PKMYT1), transcript variant 1, mRNA.
ACCESSION   NM_004203
VERSION     NM_004203.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Li,B., Huang,L. and Ruan,J.
  TITLE     PKMYT1 Promotes Epithelial-Mesenchymal Transition Process in
            Triple-Negative Breast Cancer by Activating Notch Signaling
  JOURNAL   Rev Invest Clin 76 (1), 45-59 (2024)
   PUBMED   38442372
  REMARK    GeneRIF: PKMYT1 Promotes Epithelial-Mesenchymal Transition Process
            in Triple-Negative Breast Cancer by Activating Notch Signaling.
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Fang,Y., Zhang,X., Guo,Y., Dong,Y., Liu,W., Hu,X., Li,X. and Gao,D.
  TITLE     PKMYT1: A Potential Target for CCNE1 Amplificated Colorectal Tumors
  JOURNAL   Cell Biochem Biophys 81 (3), 569-576 (2023)
   PUBMED   37572218
  REMARK    GeneRIF: PKMYT1: A Potential Target for CCNE1 Amplificated
            Colorectal Tumors.
REFERENCE   3  (bases 1 to 2061)
  AUTHORS   Yang,L., Wang,H., Guo,M., He,M., Zhang,W., Zhan,M. and Liu,Y.
  TITLE     ELF3 promotes gemcitabine resistance through PKMYT1/CDK1 signaling
            pathway in gallbladder cancer
  JOURNAL   Cell Oncol (Dordr) 46 (4), 1085-1095 (2023)
   PUBMED   36988891
  REMARK    GeneRIF: ELF3 promotes gemcitabine resistance through PKMYT1/CDK1
            signaling pathway in gallbladder cancer.
REFERENCE   4  (bases 1 to 2061)
  AUTHORS   Wang,S., Liu,X., Zhou,T., Li,J., Lin,Y., Zhou,A., Huang,J.,
            Zhao,J., Cai,J., Cai,X., Huang,Y. and Li,X.
  TITLE     PKMYT1 inhibits lung adenocarcinoma progression by abrogating AKT1
            activity
  JOURNAL   Cell Oncol (Dordr) 46 (1), 195-209 (2023)
   PUBMED   36350496
  REMARK    GeneRIF: PKMYT1 inhibits lung adenocarcinoma progression by
            abrogating AKT1 activity.
REFERENCE   5  (bases 1 to 2061)
  AUTHORS   Wu,F., Tu,C., Zhang,K., Che,H., Lin,Q., Li,Z., Zhou,Q., Tang,B.,
            Yang,Y., Chen,M. and Shao,C.
  TITLE     Knockdown of PKMYT1 is associated with autophagy inhibition and
            apoptosis induction and suppresses tumor progression in
            hepatocellular carcinoma
  JOURNAL   Biochem Biophys Res Commun 640, 173-182 (2023)
   PUBMED   36512849
  REMARK    GeneRIF: Knockdown of PKMYT1 is associated with autophagy
            inhibition and apoptosis induction and suppresses tumor progression
            in hepatocellular carcinoma.
REFERENCE   6  (bases 1 to 2061)
  AUTHORS   Wells,N.J., Watanabe,N., Tokusumi,T., Jiang,W., Verdecia,M.A. and
            Hunter,T.
  TITLE     The C-terminal domain of the Cdc2 inhibitory kinase Myt1 interacts
            with Cdc2 complexes and is required for inhibition of G(2)/M
            progression
  JOURNAL   J Cell Sci 112 (Pt 19), 3361-3371 (1999)
   PUBMED   10504341
REFERENCE   7  (bases 1 to 2061)
  AUTHORS   Liu,F., Rothblum-Oviatt,C., Ryan,C.E. and Piwnica-Worms,H.
  TITLE     Overproduction of human Myt1 kinase induces a G2 cell cycle delay
            by interfering with the intracellular trafficking of Cdc2-cyclin B1
            complexes
  JOURNAL   Mol Cell Biol 19 (7), 5113-5123 (1999)
   PUBMED   10373560
REFERENCE   8  (bases 1 to 2061)
  AUTHORS   Shen,M., Stukenberg,P.T., Kirschner,M.W. and Lu,K.P.
  TITLE     The essential mitotic peptidyl-prolyl isomerase Pin1 binds and
            regulates mitosis-specific phosphoproteins
  JOURNAL   Genes Dev 12 (5), 706-720 (1998)
   PUBMED   9499405
REFERENCE   9  (bases 1 to 2061)
  AUTHORS   Booher,R.N., Holman,P.S. and Fattaey,A.
  TITLE     Human Myt1 is a cell cycle-regulated kinase that inhibits Cdc2 but
            not Cdk2 activity
  JOURNAL   J Biol Chem 272 (35), 22300-22306 (1997)
   PUBMED   9268380
REFERENCE   10 (bases 1 to 2061)
  AUTHORS   Liu,F., Stanton,J.J., Wu,Z. and Piwnica-Worms,H.
  TITLE     The human Myt1 kinase preferentially phosphorylates Cdc2 on
            threonine 14 and localizes to the endoplasmic reticulum and Golgi
            complex
  JOURNAL   Mol Cell Biol 17 (2), 571-583 (1997)
   PUBMED   9001210
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB009060.1, BC121161.1 and
            BQ017689.1.
            
            On Nov 23, 2018 this sequence version replaced NM_004203.4.
            
            Summary: This gene encodes a member of the serine/threonine protein
            kinase family. The encoded protein is a membrane-associated kinase
            that negatively regulates the G2/M transition of the cell cycle by
            phosphorylating and inactivating cyclin-dependent kinase 1. The
            activity of the encoded protein is regulated by polo-like kinase 1.
            Alternatively spliced transcript variants encoding multiple
            isoforms have been observed for this gene. [provided by RefSeq, May
            2012].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163657.286100.1,
                                           SRR1163655.440743.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2151119, SAMEA2153307
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000262300.13/ ENSP00000262300.8
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-219               DB009060.1         76-294
            220-1870            BC121161.1         1-1651
            1871-2061           BQ017689.1         34-224              c
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..2061
                     /gene="PKMYT1"
                     /gene_synonym="MYT1; PPP1R126"
                     /note="protein kinase, membrane associated
                     tyrosine/threonine 1"
                     /db_xref="GeneID:9088"
                     /db_xref="HGNC:HGNC:29650"
                     /db_xref="MIM:602474"
     exon            1..161
                     /gene="PKMYT1"
                     /gene_synonym="MYT1; PPP1R126"
                     /inference="alignment:Splign:2.1.0"
     exon            162..426
                     /gene="PKMYT1"
                     /gene_synonym="MYT1; PPP1R126"
                     /inference="alignment:Splign:2.1.0"
     CDS             417..1916
                     /gene="PKMYT1"
                     /gene_synonym="MYT1; PPP1R126"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     membrane-associated tyrosine- and threonine-specific
                     cdc2-inhibitory kinase; myt1 kinase; protein phosphatase
                     1, regulatory subunit 126"
                     /codon_start=1
                     /product="membrane-associated tyrosine- and
                     threonine-specific cdc2-inhibitory kinase isoform 1"
                     /protein_id="NP_004194.3"
                     /db_xref="CCDS:CCDS10486.1"
                     /db_xref="GeneID:9088"
                     /db_xref="HGNC:HGNC:29650"
                     /db_xref="MIM:602474"
                     /translation="MLERPPALAMPMPTEGTPPPLSGTPIPVPAYFRHAEPGFSLKRP
                     RGLSRSLPPPPPAKGSIPISRLFPPRTPGWHQLQPRRVSFRGEASETLQSPGYDPSRP
                     ESFFQQSFQRLSRLGHGSYGEVFKVRSKEDGRLYAVKRSMSPFRGPKDRARKLAEVGS
                     HEKVGQHPCCVRLEQAWEEGGILYLQTELCGPSLQQHCEAWGASLPEAQVWGYLRDTL
                     LALAHLHSQGLVHLDVKPANIFLGPRGRCKLGDFGLLVELGTAGAGEVQEGDPRYMAP
                     ELLQGSYGTAADVFSLGLTILEVACNMELPHGGEGWQQLRQGYLPPEFTAGLSSELRS
                     VLVMMLEPDPKLRATAEALLALPVLRQPRAWGVLWCMAAEALSRGWALWQALLALLCW
                     LWHGLAHPASWLQPLGPPATPPGSPPCSLLLDSSLSSNWDDDSLGPSLSPEAVLARTV
                     GSTSTPRSRCTPRDALDLSDINSEPPRGSFPSFEPRNLLSLFEDTLDPT"
     exon            427..794
                     /gene="PKMYT1"
                     /gene_synonym="MYT1; PPP1R126"
                     /inference="alignment:Splign:2.1.0"
     exon            795..1288
                     /gene="PKMYT1"
                     /gene_synonym="MYT1; PPP1R126"
                     /inference="alignment:Splign:2.1.0"
     exon            1289..1395
                     /gene="PKMYT1"
                     /gene_synonym="MYT1; PPP1R126"
                     /inference="alignment:Splign:2.1.0"
     exon            1396..1568
                     /gene="PKMYT1"
                     /gene_synonym="MYT1; PPP1R126"
                     /inference="alignment:Splign:2.1.0"
     exon            1569..1726
                     /gene="PKMYT1"
                     /gene_synonym="MYT1; PPP1R126"
                     /inference="alignment:Splign:2.1.0"
     exon            1727..1804
                     /gene="PKMYT1"
                     /gene_synonym="MYT1; PPP1R126"
                     /inference="alignment:Splign:2.1.0"
     exon            1805..2061
                     /gene="PKMYT1"
                     /gene_synonym="MYT1; PPP1R126"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2029..2034
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PKMYT1"
                     /gene_synonym="MYT1; PPP1R126"
                     /note="hexamer: AATAAA"
     polyA_site      2061
                     /gene="PKMYT1"
                     /gene_synonym="MYT1; PPP1R126"
ORIGIN      
        1 gcgcgttcac gggccgttcc ccctcacggg agtcctccgc ccgggcgtcc ggaacagtcg
       61 acggcagact ccggcccgct gagccacccg aggggtcccg tggcctccgc ggacccggaa
      121 tctgggccct cgcggacccg cgccccgccc agtcgcccca gggcttcccc acacccacgg
      181 agtgaagtca gccgcggccc tgcctgggag gaacttaccg tctaccggga aaggtggcca
      241 gcagatgtgt cgggcctggt gagagggtga ggcgagacgg cccgatcgcc cagggccccg
      301 gaagctgcgg aggtcacccc cgcctggcct tagctcaggg acaccctgga ttcacgtggg
      361 agcccctgct cctgcctccc ccgtcccacc actgaggctg ttgggccagg ccagtcatgc
      421 tagaacggcc tcctgcactg gccatgccca tgcccacgga gggcaccccg ccacctctga
      481 gtggcacccc catcccagtc ccagcctact tccgccacgc agaacctgga ttctccctca
      541 agaggcccag ggggctcagc cggagcctcc cacctccgcc ccctgccaag ggcagcattc
      601 ccatcagccg cctcttccct cctcggaccc caggctggca ccagctgcag ccccggcggg
      661 tgtcattccg gggcgaggcc tcagagactc tgcagagccc tgggtatgac ccaagccggc
      721 cagagtcctt cttccagcag agcttccaga ggctcagccg cctgggccat ggctcctacg
      781 gagaggtctt caaggtgcgc tccaaggagg acggccggct ctatgcggta aagcgttcca
      841 tgtcaccatt ccggggcccc aaggaccggg cccgcaagtt ggccgaggtg ggcagccacg
      901 agaaggtggg gcagcaccca tgctgcgtgc ggctggagca ggcctgggag gagggcggca
      961 tcctgtacct gcagacggag ctgtgcgggc ccagcctgca gcaacactgt gaggcctggg
     1021 gtgccagcct gcctgaggcc caggtctggg gctacctgcg ggacacgctg cttgccctgg
     1081 cccatctgca cagccagggc ctggtgcacc ttgatgtcaa gcctgccaac atcttcctgg
     1141 ggccccgggg ccgctgcaag ctgggtgact tcggactgct ggtggagctg ggtacagcag
     1201 gagctggtga ggtccaggag ggagaccccc gctacatggc ccccgagctg ctgcagggct
     1261 cctatgggac agcagcggat gtgttcagtc tgggcctcac catcctggaa gtggcatgca
     1321 acatggagct gccccacggt ggggagggct ggcagcagct gcgccagggc tacctgcccc
     1381 ctgagttcac tgccggtctg tcttccgagc tgcgttctgt ccttgtcatg atgctggagc
     1441 cagaccccaa gctgcgggcc acggccgagg ccctgctggc actgcctgtg ttgaggcagc
     1501 cgcgggcctg gggtgtgctg tggtgcatgg cagcggaggc cctgagccga gggtgggccc
     1561 tgtggcaggc cctgcttgcc ctgctctgct ggctctggca tgggctggct caccctgcca
     1621 gctggctaca gcccctgggc ccgccagcca ccccgcctgg ctcaccaccc tgcagtttgc
     1681 tcctggacag cagcctctcc agcaactggg atgacgacag cctagggcct tcactctccc
     1741 ctgaggctgt cctggcccgg actgtgggga gcacctccac cccccggagc aggtgcacac
     1801 ccagggatgc cctggaccta agtgacatca actcagagcc tcctcggggc tccttcccct
     1861 cctttgagcc tcggaacctc ctcagcctgt ttgaggacac cctagaccca acctgagccc
     1921 cagactctgc ctctgcactt ttaacctttt atcctgtgtc tctcccgtcg cccttgaaag
     1981 ctggggcccc tcgggaactc ccatggtctt ctctgcctgg ccgtgtctaa taaaaagtat
     2041 ttgaaccttg ggagcaccca a
//
