LOCUS       NM_181804               1248 bp    mRNA    linear   PRI 11-JUN-2024
DEFINITION  Homo sapiens cAMP-dependent protein kinase inhibitor gamma (PKIG),
            transcript variant 3, mRNA.
ACCESSION   NM_181804
VERSION     NM_181804.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Chen,X., Hausman,B.S., Luo,G., Zhou,G., Murakami,S., Rubin,J. and
            Greenfield,E.M.
  TITLE     Protein kinase inhibitor gamma reciprocally regulates osteoblast
            and adipocyte differentiation by downregulating leukemia inhibitory
            factor
  JOURNAL   Stem Cells 31 (12), 2789-2799 (2013)
   PUBMED   23963683
  REMARK    GeneRIF: Results show that endogenous levels of Pkig reciprocally
            regulate osteoblast and adipocyte differentiation and that this
            reciprocal regulation is mediated in part by LIF.
REFERENCE   3  (bases 1 to 1248)
  AUTHORS   Zhao,L., Yang,S., Zhou,G.Q., Yang,J., Ji,D., Sabatakos,G. and
            Zhu,T.
  TITLE     Downregulation of cAMP-dependent protein kinase inhibitor gamma is
            required for BMP-2-induced osteoblastic differentiation
  JOURNAL   Int J Biochem Cell Biol 38 (12), 2064-2073 (2006)
   PUBMED   16870489
  REMARK    GeneRIF: These results indicate that the downregulation of PKIgamma
            may be prerequisite for the PKA activation during the osteoblastic
            differentiation of precursor cells.
REFERENCE   4  (bases 1 to 1248)
  AUTHORS   Yu,J., Yu,L., Chen,Z., Zheng,L., Chen,X., Wang,X., Ren,D. and
            Zhao,S.
  TITLE     Protein inhibitor of neuronal nitric oxide synthase interacts with
            protein kinase A inhibitors
  JOURNAL   Brain Res Mol Brain Res 99 (2), 145-149 (2002)
   PUBMED   11978406
REFERENCE   5  (bases 1 to 1248)
  AUTHORS   Lum,H., Hao,Z., Gayle,D., Kumar,P., Patterson,C.E. and Uhler,M.D.
  TITLE     Vascular endothelial cells express isoforms of protein kinase A
            inhibitor
  JOURNAL   Am J Physiol Cell Physiol 282 (1), C59-C66 (2002)
   PUBMED   11742798
  REMARK    GeneRIF: Functional studies of the mouse homolog
REFERENCE   6  (bases 1 to 1248)
  AUTHORS   Zheng,L., Yu,L., Tu,Q., Zhang,M., He,H., Chen,W., Gao,J., Yu,J.,
            Wu,Q. and Zhao,S.
  TITLE     Cloning and mapping of human PKIB and PKIG, and comparison of
            tissue expression patterns of three members of the protein kinase
            inhibitor family, including PKIA
  JOURNAL   Biochem J 349 (Pt 2), 403-407 (2000)
   PUBMED   10880337
REFERENCE   7  (bases 1 to 1248)
  AUTHORS   Collins,S.P. and Uhler,M.D.
  TITLE     Characterization of PKIgamma, a novel isoform of the protein kinase
            inhibitor of cAMP-dependent protein kinase
  JOURNAL   J Biol Chem 272 (29), 18169-18178 (1997)
   PUBMED   9218452
  REMARK    GeneRIF: Functional studies of the mouse homolog
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CN354542.1, CD244921.1 and
            AI140360.1.
            
            On Aug 13, 2020 this sequence version replaced NM_181804.2.
            
            Summary: This gene encodes a member of the protein kinase inhibitor
            family. Studies of a similar protein in mice suggest that this
            protein acts as a potent competitive cAMP-dependent protein kinase
            inhibitor, and is a predominant form of inhibitor in various
            tissues. The encoded protein may be involved in osteogenesis.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Jul 2013].
            
            Transcript Variant: This variant (3) differs in the 5' UTR,
            compared to variant 4. Variants 1-5 encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.988223.1,
                                           SRR14038191.430575.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-34                CN354542.1         107-140
            35-762              CD244921.1         1-728
            763-1248            AI140360.1         7-492               c
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.12"
     gene            1..1248
                     /gene="PKIG"
                     /gene_synonym="PKI-gamma"
                     /note="cAMP-dependent protein kinase inhibitor gamma"
                     /db_xref="GeneID:11142"
                     /db_xref="HGNC:HGNC:9019"
                     /db_xref="MIM:604932"
     exon            1..104
                     /gene="PKIG"
                     /gene_synonym="PKI-gamma"
                     /inference="alignment:Splign:2.1.0"
     exon            105..251
                     /gene="PKIG"
                     /gene_synonym="PKI-gamma"
                     /inference="alignment:Splign:2.1.0"
     exon            252..321
                     /gene="PKIG"
                     /gene_synonym="PKI-gamma"
                     /inference="alignment:Splign:2.1.0"
     exon            322..495
                     /gene="PKIG"
                     /gene_synonym="PKI-gamma"
                     /inference="alignment:Splign:2.1.0"
     CDS             345..575
                     /gene="PKIG"
                     /gene_synonym="PKI-gamma"
                     /note="protein kinase (cAMP-dependent, catalytic)
                     inhibitor gamma"
                     /codon_start=1
                     /product="cAMP-dependent protein kinase inhibitor gamma"
                     /protein_id="NP_861520.1"
                     /db_xref="CCDS:CCDS13334.1"
                     /db_xref="GeneID:11142"
                     /db_xref="HGNC:HGNC:9019"
                     /db_xref="MIM:604932"
                     /translation="MMEVESSYSDFISCDRTGRRNAVPDIQGDSEAVSVRKLAGDMGE
                     LALEGAEGQVEGSAPDKEAGNQPQSSDGTTSS"
     exon            496..1248
                     /gene="PKIG"
                     /gene_synonym="PKI-gamma"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1225..1230
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PKIG"
                     /gene_synonym="PKI-gamma"
                     /note="hexamer: AATAAA"
     polyA_site      1248
                     /gene="PKIG"
                     /gene_synonym="PKI-gamma"
                     /note="major polyA site"
ORIGIN      
        1 aaggcaacgg gctccgctgc gggttgcggg ctgcgggcgc taggctgccc cggggaggcg
       61 ctgcggaccg gcggcccagg cacggagagg cggcgagacc gcaggagaca cggggaagag
      121 acagagagga gggtaaagtg agaatcctgc ccgcaccaca ggtctggact gctaaccttg
      181 aatcctggtt tcttaaattc tgcctccgct tctgactgag gaccactgga ttttgaggaa
      241 actttggtct taattcccgt actgattagt caacagtgga aaatctgaag agatgcaagc
      301 aggaaaaaga aattaaacca ggcctgagga gcgatgcgac aggcatgatg gaggtcgagt
      361 cctcctactc ggacttcatc tcctgtgacc ggacaggccg tcggaatgcg gtccctgaca
      421 tccagggaga ctcagaggct gtgagcgtga ggaagctggc tggagacatg ggcgagctgg
      481 cactcgaggg ggcagaagga caggtggagg gaagcgcccc agacaaggaa gctggcaacc
      541 agccccagag cagcgatggg accacctcgt cttgaatctg accttgtcca agaaggctgg
      601 acgagagacc ttctgtcccc tcccagaggg ggaaccctgg cactggccca gcagcctctt
      661 ctctgagctc catgtcccag ataaaccagg ccagactgag aaggctcccc agaggcctct
      721 gtggcctcca ctccgggaaa gccctctgcc cacacccaca ggcttcacat tcccaccacc
      781 ttcgcaccgt gcccaggtac actttcaaga cactgtaacc acaagatgtt atttattgag
      841 ctggcgccgg gacttgggcg gggcctgccc tacagtgagc agcccacaca ggaacgctcc
      901 tctcgcgagc ggcccgggca gggaccctgt cccaacacca acacctcctc tccagcccaa
      961 tcttctgggt ccagacctgc ttgtcccttt tttagaaaac acttttaaac tttttaaaaa
     1021 ttttaaacct tttttcagca gatatggaga gagctgacaa tcaattcaca ttttttaagc
     1081 cattttagct aaactgtcat tgtgcatctc tgaggttccc tcatggagct ccacagatcc
     1141 atttttaggg aagggatttt ggctcaaaac gatctgacca cctctgccct gtccaccagg
     1201 ataagtgaca cctaggaccc aggaaataaa tgccgatgat ttgtgtga
//
